LOCUS       DATDSU010000001         7515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_336069, whole genome shotgun sequence.
ACCESSION   DATDSU010000001 DATDSU010000000
VERSION     DATDSU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336069"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1942)
                     /gene="argS"
                     /locus_tag="VI981_00005"
     CDS             complement(209..1942)
                     /gene="argS"
                     /locus_tag="VI981_00005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY4478735.1"
                     /translation="MPQISSKLNTLIKQALKDLSIQIDDIHLEHPAEIEHGDFSTNVA
                     LALAKTEKKNPRELAKDIAAKIASYQDETIERVETAGAGFVNFFLSRSFYTRVIESGL
                     QAPERIGHTEALRNKKVLIEYTDPNAFKVFHIGHLMANAIGESVARTLEFSGASLVRL
                     CYPSDIGLHIAKAIWGMKKNEHDSPKETDSLTAKTNFLGTAYVLGTKMYEEDPVVKEE
                     INGLNKKLFEKSDPAVNRLYEKGREWSLDHFEELYKKLGTKFDRYIFESEVADKGRLI
                     VEEYLGRGCFEKSEGAIVFKGEEHGLHTRVFITAKGLPTYESKEIGLNFKKFELYPCT
                     DSSIIITGNEQNEYFKVLKRVLSIIAPNIGEKTHHVSHGMMRFADGKMSSRTGNVVTA
                     EHLLSAVEALVHQKIADRELVDNEKNKIADIVAVGAIKFSILRQAIGGDIIYDFDKSI
                     SFEGDSGPYLQYSYTRARSVIEKAKGVGMKTHLETKSPSLNVEVYVLEKWLYRFPEVV
                     TRAGQEFAPHYLATYLLELSSAFNAFYAEHKIVDLDDSEAPYRLALTEVFAATMKNGL
                     YLLGIKAPERM"
     gene            complement(1997..2500)
                     /locus_tag="VI981_00010"
     CDS             complement(1997..2500)
                     /locus_tag="VI981_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478736.1"
                     /translation="MSTKATDVTLESFLHGTLTIEIAGIGKETCNASELPLFRRASLA
                     LGRLGQGLECDPRKVFSVESYDSAAKTIRLKLPPSAPVSPLEVIDLAFLSAKMESEKQ
                     HSGRARLRQTVAVAKEPVLCFSRLRYAGGGYCLTCYSRASLKEAETDQGFKFLRFCPD
                     CREYFRT"
     gene            complement(2578..3171)
                     /locus_tag="VI981_00015"
     CDS             complement(2578..3171)
                     /locus_tag="VI981_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4478737.1"
                     /translation="MKKRDIDFLFEIGTLRKVPRAWQQVLNGKVENIAEHIFRTALIA
                     WTIAKREKADVAKVLRIALVHDLAETRATDIAFLHRDYVTRHEELAQKDIFEDTSLGD
                     EALSLLKEYEERESLEARIVKDADNLDVDLELRELAQNGDTSAAGMRKQHRPHVREVK
                     LYTKTAKKMWDQIQKTGPNNWHQALTNKWIKNKRGAR"
     gene            complement(3190..3678)
                     /locus_tag="VI981_00020"
     CDS             complement(3190..3678)
                     /locus_tag="VI981_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478738.1"
                     /translation="MKPSKILFWVVITGIVVMVFVWISKSSDRYDGNKDQFKKNKEES
                     SESILYQSLYGAPVWCDKTKEKCYSKLTLFYDGNLIRDSGTKDAPPMSKIITASDIAR
                     VIDLINKTGILQKRCTRDYEKVIYETSELITISVGGETVHPSTDGCSNEVYSIESFVQ
                     SL"
     gene            complement(3700..4878)
                     /gene="glyA"
                     /locus_tag="VI981_00025"
     CDS             complement(3700..4878)
                     /gene="glyA"
                     /locus_tag="VI981_00025"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY4478739.1"
                     /translation="MKDTQIEKLIKSEAKRQKKVINLIASENYVSRDVLKALGSELTN
                     KYAEGYPGRRYYRGTKIVDEIENLARERALKLFGLEEIKWSVNVQPLSGSPANLAVYA
                     GVVPLGEKIMGMELSHGGHLTHGQTVSVTGKFWKRVPYGVDKKTERLDYEEIKKIAVS
                     EKPKIIVAGFTAYAHCIDFKKFREIADACGAILMVDMSHFGGLVAGRAYPSPFDFADV
                     VTTTTHKTLRGPRSAIIFVKKDERELDKHINKALFPGLQGGPHLNQIAGVAVALREAM
                     SPAFQKYARQVIKNAQALTDELSKLGWRVVEGGTETHLLRVDVWQDGKGLSGRAAADK
                     LEAAGIIVNENTIPFDTRKPLDPSGIRLGSAAETTRGKKEKDFKAIAKKIDKVLRAKN
                     "
     gene            complement(5146..5658)
                     /locus_tag="VI981_00030"
     CDS             complement(5146..5658)
                     /locus_tag="VI981_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478740.1"
                     /translation="MENLYLKAIRLGNPTSEEFASLQLALDEGESDDFFRDDVLEIVG
                     SFVQSSLRISFDPLAHLRLIFPLYEWGSYDVIEGKRGASSEVLREFRDLIQSADFFWP
                     AACTADDWVIARRKDRVGKGLSLCGSKELLASVTLMSESQESSVSNLARMLEDPAALF
                     NKVLKDQADI"
     gene            5854..6426
                     /gene="cas2"
                     /locus_tag="VI981_00035"
     CDS             5854..6426
                     /gene="cas2"
                     /locus_tag="VI981_00035"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEY4478741.1"
                     /translation="MGKIEIESRKRSRNRNIQKIILQSIAAAGLLSVALVAPNSLKLL
                     KDLGIVPKRRQREIIRASRDRLVKHGLLFRNEKGYLRLTAKGEAKLRQLELCDFKLKF
                     PKRWDKKWRVLIFDISTKKRGLRDKVRNTLKTIGFYKLQHSVWVFPHSCEDLITLLKA
                     DFKIGDDLIYMIVDSIENDKKVRDHFYLKQ"
     gene            complement(6490..6918)
                     /gene="tnpA"
                     /locus_tag="VI981_00040"
     CDS             complement(6490..6918)
                     /gene="tnpA"
                     /locus_tag="VI981_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY4478742.1"
                     /translation="MTKTHHNVYTTHYHIVFPVKYRKALLTDEIPLAIVAIAQEIALR
                     YDIEFEQIGCDETHIHLLVSFPPKHSGSEMVGMFKSITAKQLFLKFPALKKELWSGEF
                     WSDGFYMATVGERGNWHAVEKYVAEQGKTMDIPQQLRLLT"
     gene            7157..>7515
                     /locus_tag="VI981_00045"
     CDS             7157..>7515
                     /locus_tag="VI981_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY4478743.1"
                     /translation="MKPLIERTVDAQYQNLLRNILEKGVQTKSQQGVDALTLLGPSPL
                     HFKLDNGFPIINERSLNPKTSTTLPVTVWEQAIAEILAFINGARTQEEFVSFGCHWWR
                     WWVTKEKCEKRGLKEVDL"
BASE COUNT         1977 a         1868 c         1581 g         2089 t
ORIGIN      
        1 aaagcttatt gtcacagcat gtgattccct ttccgccggg aagccttatt tcgatttgtc
       61 catccttgcc gcagggacag cgccgctgcc acaaacgttt tctgcgaatt gaaacttttt
      121 gatacatagt ccctctcccg atacgatacc accatcggga acaacgctca atacagagac
      181 ttccaaaaaa cttcgagcca agttagagct acatcctctc cggcgccttg atgccgagaa
      241 gatacagtcc gtttttcatc gtggcggcga aaacttcggt tagcgcgaga cgatacggag
      301 cttcggagtc atccaaatca actattttgt gctccgcgta aaacgcattg aaggcagaag
      361 ataattccaa gagataggtc gcaaggtagt gaggcgcaaa ttcctgtccc gcccgcgtca
      421 ctacttcagg aaatctgtag agccacttct caagtacata cacctccaca tttagacttg
      481 gagacttagt ctccaagtga gttttcattc caaccccttt tgccttttca ataacagatc
      541 gagcacgtgt atatgaatac tgaagatacg gccccgaatc accctcaaat gaaatagatt
      601 tatcaaaatc ataaatgatg tccccgccaa tggcctgccg caaaatagaa aacttaatcg
      661 ctcccaccgc cacaatgtcc gcaatcttat ttttctcgtt gtccacgagc tctcgatcgg
      721 cgattttctg atgcacgaga gcctcgacgg cggaaagcaa gtgttcggcg gtcaccacat
      781 tgccggtgcg tgaagacatt tttccgtcgg caaaacgcat catgccgtgc gacacatggt
      841 gcgttttttc accgatattt ggagcgataa tggagagcac gcgcttcagc actttgaaat
      901 attcgttctg ttcgttccct gtaatgatga tcgaagagtc agtacatgga tagagttcga
      961 atttcttaaa attaaggcct atttccttcg actcatacgt cggtaggccc tttgccgtga
     1021 tgaaaactct cgtgtgaagt ccgtgctcct cgcccttaaa cacaatggct ccctcacttt
     1081 tttcgaaaca gcccctgccc agatactcct cgacaatcag tctgcctttg tccgccactt
     1141 cgctttcaaa aatatatcgg tcaaatttag tgccaagttt cttgtagagt tcttcaaagt
     1201 ggtcaagact ccactctctt cccttttcat agagtcggtt taccgcaggg tcgctttttt
     1261 caaaaagttt tttattcagc ccgtttattt cctcttttac taccggatct tcttcataca
     1321 tttttgtgcc gagcacatag gccgttccca aaaagtttgt tttggccgtc agagaatccg
     1381 tctccttcgg ggaatcgtgc tcgtttttct tcatgcccca gatcgccttg gcaatatgca
     1441 aaccaatgtc gctcggatag cacaaacgca cgagcgacgc gcctgaaaat tcgagtgtgc
     1501 gggctaccga ttcgccgatc gcgttcgcca tcaggtgacc aatatgaaaa accttgaacg
     1561 cgttcggatc ggtatattcg ataaggactt ttttgtttcg taatgcttcg gtatgcccga
     1621 tgcgttctgg cgcctgcagt cccgattcaa tgacacgcgt atagaaggaa cgggacagaa
     1681 agaaattgac aaacccggcc ccggccgttt ccacgcgttc aatggtttcg tcctggtacg
     1741 acgcgatttt tgctgcaatg tccttcgcca gttctcgagg atttttcttc tccgtcttgg
     1801 caagcgcaag tgctacgttg gtcgaaaaat cgccgtgttc aatttccgcc ggatgttcga
     1861 ggtgtatgtc atctatctga atggacaggt ctttaagcgc ctgtttaatt agagtattta
     1921 atttcgagga aatctgaggc atggatgaat catatcttaa aattacagca aagaaaaaag
     1981 cgccaccaag gtgacgctac gttcgaaagt actctctgca gtccggacaa aatcgcaaaa
     2041 acttaaaccc ctgatccgtc tccgcttcct tgagcgatgc cctgctgtaa catgtgagac
     2101 agtaaccgcc gcccgcataa cgcaagcggc tgaagcagag caccggctcc tttgccaccg
     2161 caaccgtctg cctcaaacgc gcacggccgc tgtgctgttt ctccgactcc attttggcgg
     2221 agagaaatgc taggtcgata acttcaagag gagagaccgg cgcggaaggc gggagcttga
     2281 gtcgtatcgt cttggccgca gagtcgtatg actcaacgga aaatactttt cgcggatcgc
     2341 attcaagtcc ttgtccgagc ctccccaaag caagggaagc tctccggaaa agcggcagct
     2401 cggaagcatt gcaggtttcc tttccaatgc cagcgatttc gatggtcagc gttccatgta
     2461 agaacgattc aagtgtgaca tccgtggctt tcgtcgacat gtgtgtcctt ggttgattgt
     2521 tgttccaaaa acaagggtag ctccagcttt accccaagtc aagcagttat aaaaatatca
     2581 tcttgccccc cttttgttct ttatccattt atttgtaagc gcctgatgcc agttgttggg
     2641 accggttttt tgaatttgat cccacatttt ctttgcagtt tttgtgtata gctttacttc
     2701 cctgacgtgg ggacgatgtt gttttcgcat tcctgcggca gaagtgtcac cattttgagc
     2761 gagctctcga agctcaagat caacgtccag attgtccgca tctttgacaa ttcttgcctc
     2821 aagactttct cgctcttcat attctttaag cagtgagagc gcctcgtcgc ccaaagaagt
     2881 gtcctcgaaa atatcttttt gcgccagctc ttcgtgccgt gtcacgtaat ctcggtgtaa
     2941 aaaagcgatg tcagtagccc gagtttctgc taaatcatga accagcgcaa tgcgcaagac
     3001 tttagcaacg tcagcttttt cccgtttcgc gatcgtccaa gcaatgagag ccgtgcgaaa
     3061 tatatgctcc gcaatatttt caactttccc attcaaaacc tgctgccagg cacgcggcac
     3121 tttgcgcaac gttccaatct caaacaaaaa atctatgtct cgttttttca tgtttttctg
     3181 gttaacttct catagcgact gtacaaaact ttcaatacta tagacttcgt tcgagcaacc
     3241 gtcagtactc ggatgtacag tttctccgcc aactgagatc gttatcagtt cgctagtttc
     3301 atatatgact ttttcataat cacgcgtgca acgtttttgt agtatgccgg tcttattaat
     3361 gagatctatt acacgagcaa tatcagatgc agtgattatt ttactcattg gcggcgcatc
     3421 cttcgtcccg gaatcacgaa ttaaattacc gtcataaaaa agggtcaatt tagagtagca
     3481 cttctctttg gttttatcac accatacagg tgccccatat aaactttggt aaagaataga
     3541 ttctgaagat tcctctttat tttttttgaa ttgatcttta tttccgtcat acctatcaga
     3601 actttttgaa atccaaacga aaaccatgac aacgattcct gttattacta cccagaatag
     3661 aatttttgac ggtttcatag aggatactcc tttcgcagat taattcttgg cccgcaatac
     3721 cttatctatc ttcttcgcaa tcgctttgaa atctttttcc tttttgcccc gtgttgtttc
     3781 agcggccgag ccgagacgaa tacctgaagg atcgagcggt ttacgcgtat cgaacggaat
     3841 tgtattttcg ttcacaataa ttcctgcggc ttccagttta tctgccgccg ctctaccgct
     3901 taggccttta ccgtcctgcc aaacgtcaac gcgaagcagg tgtgtttctg tcccgccctc
     3961 aacgacgcgc cagccaagtt ttgaaagttc gtccgtaaga gcttgagcat ttttgatgac
     4021 ttgtcttgca tatttctgaa acgccggaga catcgcttct ctaagagcaa ccgcaacccc
     4081 tgcgatttgg tttaaatgag ggccaccctg cagccccggg aagagcgctt tatttatatg
     4141 tttgtcgagt tctcgttcat ctttttttac aaagattata gcgctccgcg gccctcgaag
     4201 cgttttgtgg gttgttgtcg tcactacatc ggcaaagtca aaaggtgatg gatacgcgcg
     4261 acctgccaca agacctccaa aatgggacat gtcaaccatc aatatcgcac cgcacgcatc
     4321 cgcaatttct ctgaattttt tgaaatcaat acaatgagca tatgccgtaa aacccgccac
     4381 aattattttt ggcttttccg aaacagcaat tttttttatt tcttcatagt cgagacgctc
     4441 tgtttttttg tcgactccgt agggcacccg tttccaaaat ttccccgtca cggacaccgt
     4501 ttggccatgt gtcagatggc cgccatgcga aagttccatg cccattattt tttcaccaag
     4561 cggcacaacg cccgcgtata cggccaagtt tgcgggagaa cctgaaagag gctgaacatt
     4621 tacgctccat tttatttctt caagaccaaa aagtttaaga gcccgctctc gagcaagatt
     4681 ttcaatttca tcgactatct tggtcccccg ataatagcgg cgtcccggat aaccctcggc
     4741 atatttattg gtcagttccg aaccgagggc ctttaaaaca tcgcgagaga cgtagttctc
     4801 tgaagctatg aggtttatta ctttcttctg gcgtttcgcc tcactcttta tgagtttttc
     4861 gatttgtgtg tccttcatcc cgttagtgac aggtcgcgat ttatcgcatc ctgcaagtta
     4921 gagtttctaa agaaaaatta ctaattcgat cccgtactaa accccaatcg cgaccgcggt
     4981 cactaacggg attcatttga atataatacc aatatacaaa tttatactaa tgatactaat
     5041 aatacgaatc aaatgtgaga gcgtcagatc ataggtttgc ttagtgtccg cattgtagca
     5101 tttggataaa aataaaaaag cacccaaagc ttgggtgcta ctcaactaaa tatcggcctg
     5161 atccttgaga actttgttga agagagctgc aggatcctca agcatcctgg ctaagttcga
     5221 taccgaggac tcctgtgatt cagacattag agtcacagat gctaaaagct cctttgagcc
     5281 gcacaaagaa agccctttac cgacgcgatc ctttctcctt gcaataaccc agtcatccgc
     5341 ggtacaagcg gctggccaaa aaaaatcggc gctctgtata agatctcgaa attcacgtaa
     5401 aacttctgag ctcgctcctc tcttaccttc gatcacgtca taagatcccc actcataaag
     5461 cggaaagatg aggcgcaagt gcgccaaagg atcaaacgag atacgcaggg aactctgcac
     5521 aaaagatccc acgatctcta gtacatcgtc ccgaaaaaag tcatcgctct caccttcgtc
     5581 gagagcaagc tgaagactgg caaattcttc cgaagttgga tttccaagcc tgattgcttt
     5641 caagtacaga ttctccacag tctcgcggac tagctcactt tggtttctct cgctcgcccc
     5701 gcgctgcggg actcgcgaaa ttcaccttac gttaagcctt agtgactagc ttattgcaaa
     5761 gaaccattcc tcacaactgt tccgaaacta ccacatttct tgctatttcg tcaatataca
     5821 tttaattttc gcacgtgcgt ataattactg tatatgggta aaattgaaat tgaatctaga
     5881 aagagatcta gaaacagaaa cattcagaaa ataatcctcc aatcaatcgc tgctgcaggc
     5941 ctcttaagtg tagcccttgt cgctccaaat tctttaaaac tcctcaaaga tctcggtatt
     6001 gtccccaaac gtcggcaacg agaaataata cgcgcttcac gtgacaggtt agttaaacac
     6061 ggtctcctgt tcagaaacga gaaaggatat ttaaggctga ctgcaaaggg agaggcaaag
     6121 ttacgacaac ttgagctctg cgatttcaag ctaaagtttc ccaaaagatg ggacaaaaag
     6181 tggcgagttt tgatttttga catttcgaca aaaaaaagag gccttcgtga caaagtccga
     6241 aacaccttaa aaaccattgg tttttacaaa ttgcagcata gtgtctgggt gttcccccac
     6301 tcgtgcgaag atctaatcac gcttcttaag gcagacttca aaattggaga cgatctcatc
     6361 tatatgatcg tggactcaat cgaaaatgat aaaaaagtcc gcgatcactt ttatcttaaa
     6421 cagtaaagta actccatttc aatcaattac ctcggggcag agcccacgag gtatgactcg
     6481 aggtctggcc taagtgagta atctaagctg ttgcgggata tccatcgtct ttccctgctc
     6541 cgcaacatat ttctcgaccg catgccaatt tccccgctcg ccgacggttg ccatatagaa
     6601 gccatcactc cagaactcac cgctccagag ctcttttttg agcgcaggaa atttgaggaa
     6661 taactgtttg gcagtgatac ttttgaacat tcccaccatt tctgatccgc tgtgttttgg
     6721 aggaaaagag accaagagat gtatatgggt ttcgtcacag ccaatttgtt caaattcaat
     6781 gtcataacgg agggcaattt cctgcgcaat cgcaacgatt gcgagcggga tttcatctgt
     6841 aagcagggct ttacggtatt tgacgggaaa aacgatgtgg tagtgcgttg tatagacatt
     6901 gtggtgagtt tttgtcacgg ccattcctca attgtaagtt ggaaaccgaa ggtttccaaa
     6961 ccttcctaca aggatcccgc ggcagagccg cggggaaatc cgcttcgcgt attatactac
     7021 ttaaaaaatc tagaaatttt gagaagtttg gacgaaccga cctacattaa ctttacgcac
     7081 gtgcgtaaag ttaatgtagg tggaaaataa attgagaata ctggtattat tggaaggtta
     7141 ttattcgtca aaaattatga aaccactcat cgaacggacc gtcgacgctc aataccaaaa
     7201 cctccttaga aacattctcg aaaaaggagt gcaaacaaaa agccagcaag gtgttgatgc
     7261 actgacacta ttgggtccaa gtccccttca cttcaagctc gacaacggct tccccatcat
     7321 caacgagcga agcctgaacc caaagacctc cacaaccctg ccagtgacgg tgtgggagca
     7381 ggccatcgcc gagatccttg cctttataaa cggcgcccga acgcaagagg aattcgtatc
     7441 attcggctgt cattggtgga gatggtgggt cacgaaagaa aagtgcgaaa aacgcggtct
     7501 caaggaagta gatct
//
LOCUS       DATDSU010000002         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1005545, whole genome shotgun sequence.
ACCESSION   DATDSU010000002 DATDSU010000000
VERSION     DATDSU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005545"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..930)
                     /locus_tag="VI981_00050"
     CDS             complement(85..930)
                     /locus_tag="VI981_00050"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009633210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HEY4478744.1"
                     /translation="MTEKQKMKAAIQSVIKAMMDRVMHKVLVEDPFVKEKLQADKPLY
                     TALVPDEIFKGSHFERRFVTPFGGVWEKLAVVAATEGLGYGEQGFAITGKVNKERLRR
                     IQEVLNRLEHPENGKRVKPNWEEELKYVLEGKSDKLIPTTVICDVYAVDKKANKKYAF
                     ELKAPLPNSDQTKVSKEKILKLYAMEPLLVDGAYYALPYNPYGKREDYAWAFPARWFD
                     MKKDEVVLIGTEFWEKIGGTGTYQAFIDAVNEIGKEYKDRIYREFLGMEPPEEKLKEG
                     LLDGK"
     gene            complement(927..2120)
                     /locus_tag="VI981_00055"
     CDS             complement(927..2120)
                     /locus_tag="VI981_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY4478745.1"
                     /translation="MYDNVIAQKIATQYNEQPLTDFESVVPETALDSLNLNWRERDLP
                     EYQRTKHVHRLHPYLGKFIPQLVEIFLRKYRPTLVYDPFAGSGTTLVEAHALGIDSIG
                     TDISVFNVLLSRVKTADYNIPLLEKEIHDILKRLDTYKSKFATDEKVNKLFFSKNEYI
                     QKWFAPNTQKELLCYARLIKDYTYQDLLRVVLSRSARSARLVTHYDLDFPKEPQINPY
                     QCYKHNRTCQPVEEAHKFLSRYTIDTLDRIKEFSKIKTKASVVVNHADSREVVLPKAI
                     DMVFTSPPYIGLIDYHEQHKYAYELLGLKNNETKEIGPAKNGQSQQAKQDYIKGINDV
                     MIHTRDAMTKNGLALIVVNDKYGLYKPEAAGFKEVGRVERHVNRRTGRREGAFYESIL
                     IWQKA"
BASE COUNT          507 a          564 c          477 g          681 t
ORIGIN      
        1 gtcaacacgt gcgtggtgct acgtagcaat gtaaggagca tgctatgtag tatctgttgt
       61 ggtgccgcaa taggaattgc ggccttactt tccatcaagc aaaccctctt tcaatttttc
      121 ctccggtggc tccatcccca agaactcgcg gtagattcta tccttgtatt cttttccgat
      181 ttcgttgacg gcatcaataa acgcttgata tgttcctgtg ccgccaatct tttcccaaaa
      241 ttccgttcca atcaaaacca cctcgtcttt tttcatgtca aaccagcgtg caggaaatgc
      301 ccacgcataa tcttctcgct tgccgtacgg attatatggg agcgcgtaat atgcaccgtc
      361 aacaaggagc ggctccatcg cgtacagttt caagattttt tccttactca cttttgtctg
      421 gtcgctattc ggtagaggag ctttcaactc gaaggcgtac tttttgtttg ctttcttatc
      481 caccgcgtag acatcgcaaa tgaccgttgt cggaatcagt ttgtcgcttt ttccctccaa
      541 cacgtacttc aattcctcct cccaatttgg cttcactctc ttgccatttt cggggtgttc
      601 caaacgattc aatacttcct ggattctgcg tagccgttct ttgttcacct tgccagttat
      661 cgcaaaaccc tgctcgccat accccagccc ctccgttgcc gcgaccaccg cgagtttctc
      721 ccacacacca ccgaaaggtg tcacgaagcg tctttcaaag tgcgaacctt tgaatatctc
      781 atccggaaca agcgccgtat acaatggctt gtctgcttgc agtttttctt tcacgaacgg
      841 atcttccaca agaaccttgt gcatgaccct gtccatcatc gctttgatga cggattgaat
      901 ggccgctttc attttctgtt tctccgtcat gccttttgcc atattaggat actttcataa
      961 aaagcccctt cgcgccgtcc tgtgcgacga ttgacatgtc gctcgactct accaacttcc
     1021 ttaaaaccag ccgcttccgg tttatacaac ccgtacttat cgttgacaac aataagggca
     1081 agtccatttt ttgtcatggc gtctcgggtg tgaatcatta catcgtttat acccttgatg
     1141 tagtcctgct ttgcttgttg cgattgtcca ttttttgcgg gcccgatttc tttcgtttcg
     1201 ttgttcttca agccaagcaa ttcataagcg tatttgtgct gttcgtgata atcaattagg
     1261 ccgatatacg gaggcgaagt aaaaaccata tcaatcgctt tcggcaacac cacttcgcgt
     1321 gaatcggcgt gatttactac aacactcgct ttagttttta tctttgaaaa ttctttaatc
     1381 cggtcgagtg tgtcaatcgt ataacgcgat aagaatttat gtgcttcctc aaccggctga
     1441 caagtccggt tgtgtttata gcattgatac gggtttatct gaggctcttt tggaaaatcc
     1501 aaatcgtaat gcgtaacgag ccgcgccgaa cgcgccgaac gcgagagtac gactctcaac
     1561 aagtcttgat aggtgtagtc cttgattagt cgcgcatagc acaaaagttc tttctgcgtg
     1621 ttcggcgcaa accacttctg tatgtattcg tttttgctga aaaataattt atttaccttt
     1681 tcatccgttg caaatttgct tttgtaagta tcaagccgct ttaggatgtc atgaatctct
     1741 ttttcaagga gcgggatatt gtagtcggcc gttttgacgc gagagagcag gacgttgaac
     1801 acggatatat ccgtgccaat ggaatctatg ccgagcgcgt gtgcctccac cagagtcgtt
     1861 ccacttcctg caaatggatc gtaaacaagt gtcgggcggt actttctcaa aaaaatctca
     1921 acgagttgag gtatgaattt cccgagatat ggatgcaggc ggtggacgtg ttttgtccgc
     1981 tgatattcag gcaaatcacg ctcgcgccaa ttcaagttta aggagtcgag agctgtttca
     2041 ggcacgacac tttcaaaatc tgtcaacggt tgctcgttat actgggtggc aatcttttgt
     2101 gcgatgacat tgtcgtacat gtgtctagga ttatacctca tttcgacccc gccccgccgc
     2161 cattcaattt tgcattcccc ccgcgaaaaa tacgaaagaa tggaaagcgg cggggcgcgg
     2221 gttcgaggg
//
LOCUS       DATDSU010000003         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1507386, whole genome shotgun sequence.
ACCESSION   DATDSU010000003 DATDSU010000000
VERSION     DATDSU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507386"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VI981_00060"
     CDS             <1..183
                     /locus_tag="VI981_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478746.1"
                     /translation="SYYAMNISNISSILSNGVKDKIFPGAVLSISINGTSPYQMAVGN
                     FTYDQNSPSCVIINNF"
     gene            194..1060
                     /locus_tag="VI981_00065"
     CDS             194..1060
                     /locus_tag="VI981_00065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4478747.1"
                     /translation="MTKPIVATVALKLLELEKIKLDSTIGEYLPQLAGTNVGSGITVW
                     NLLTHTSSINLKMASLADEALGKEAFFNALITYNAKLDPSTQVNYANVNTFLLGEITT
                     VVSGIPLDVLVRKEVTEPLGMVDTTFVPSPDITERIVPTEIIDTVIIRGVVHDPSVRG
                     IGGIAGHAGLFSTISDLHNFLTMWTGGGVFKGKRVLAKNIVSQALVNQTPKFNKSSCL
                     GWHLDNPDYLGNLASRDTFFHPGFTGTIIVGNILRKERIVFLSNCTFPHRENHHIKNE
                     FIKKLFDAIFSS"
     gene            complement(1050..2048)
                     /locus_tag="VI981_00070"
     CDS             complement(1050..2048)
                     /locus_tag="VI981_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY4478748.1"
                     /translation="MRKITNTNEQTLLKSCNEIYGLDAARASVLRDGSDNIIWTLEIK
                     NGKKYVARMSKRELGGDIAFEAEWLKVLLADGVPVVPIIETKDKKPFAILPTGKTLTI
                     FEFVEGKHLPFGIDQPPPLSAVKSAAEALAKLHNVSRRHDISLSRKRTVFTELERVIA
                     KQKEVEEKVSSGKEFVAEVEKILSWGKNQKFTPVLIHNDYRTGNLLFDENDKVAAILD
                     FDWSCQGPAIKDVAHALVEWSFPDGAKQHWREVFDTFLDSYNRHAEEIIKHDDNLKNW
                     ILFSCLSDTATYIIDRLERGEIKPVAGAFMYQKFQYFKKTTTRGTSIMMFPRFFMN"
     gene            2214..3440
                     /locus_tag="VI981_00075"
     CDS             2214..3440
                     /locus_tag="VI981_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY4478749.1"
                     /translation="MKNLSITFVPRLGELEVEVVERKGIGHPDTIADALAESLSVAYA
                     RYTLGHFGAILHHNFDKLAVLGGTTEVDFGTGKLTSPIRVILNGRASMRFGDKAIPVR
                     DILEAETQRFFSSLFGDSLNAERDIEKHWFVSSASGPGKTRWSTGSRAHLFTPRSISE
                     VKGYDRLLNNDTSMGCGYAPLSPVETAVLALEGKLNSSDMKQKHLWLGTDIKVMAVKT
                     HGQCGVTICLPQIAQYVSSLESYQDNLAAMQSIIEEILGELLPGMRIALSTNMRDNIA
                     HGDVYLTAIGSSIESGDEGIVGRGNRINGLITPLRPINLEGACGKNPKYYTGKLYNVA
                     AHKIAHRLYGETRKACEVLLVGQTGQPVVEPWHTLIRTAETPLDEKRIKLIAEEELTR
                     IPQITEALLERKVRLY"
BASE COUNT         1011 a          788 c          796 g          969 t
ORIGIN      
        1 agttattatg ccatgaacat ttcaaacatt tcgtccattt tgagtaatgg agttaaggat
       61 aaaatttttc ccggcgctgt tctatcgatc tcaattaacg ggacttctcc atatcaaatg
      121 gcagtcggga attttaccta tgatcaaaac agtccaagtt gtgtcattat caacaatttt
      181 tgatatagcg tctgtgacta aaccaatcgt cgcgacagtt gccctaaaat tattagaact
      241 agaaaagata aaactggata gcactattgg ggaatactta cctcagcttg cagggacaaa
      301 tgtcggaagt ggcattactg tttggaattt gttaactcac acaagttcga taaatttaaa
      361 aatggcttct ttggccgacg aagccttagg taaggaagct tttttcaatg cccttattac
      421 atataatgca aagttggatc ccagcaccca ggttaattac gcaaatgtta atactttttt
      481 gctgggggaa ataacaactg ttgtctcggg tattcctctt gatgtacttg ttagaaaaga
      541 agtaaccgaa ccattgggga tggttgatac aacatttgtt ccctcaccag atatcactga
      601 aaggatcgtt ccgacggaaa ttattgatac tgtgatcatt cgcggtgtcg tacatgatcc
      661 aagcgtaaga ggaattggcg gaattgccgg acacgccgga ctattttcca ctatatcaga
      721 tttgcataat tttttaacca tgtggacagg agggggtgtt ttcaaaggaa agcgagtatt
      781 agctaaaaat atcgtcagtc aggcactcgt gaatcaaaca cctaagttta acaagtcatc
      841 ttgcttgggc tggcatcttg ataatcctga ttacttgggt aatctagcgt cgagggacac
      901 gtttttccat cctggtttta ctgggacaat tatcgttggt aacattttac gaaaggaaag
      961 aatagttttt ttatcaaact gcacttttcc acatagagag aatcatcaca ttaaaaatga
     1021 atttattaaa aagttatttg acgctatttt tagttcataa aaaagcgcgg gaacatcata
     1081 atagatgtcc cgcgcgtcgt tgttttttta aaatactgaa atttctgata cataaacgcc
     1141 ccggcaactg gttttatttc cccgcgctct aaacggtcaa ttatgtaagt ggcggtgtcg
     1201 gaaagacaac tgaatagaat ccagttttta agattgtcat catgtttgat aatttcttcg
     1261 gcgtggcgat tgtagctatc caaaaaagta tcaaagacct ctcgccaatg ctgtttagca
     1321 ccatcgggaa aagaccattc aacaagggca tgggcgacat ctttgattgc cggaccttgg
     1381 caactccaat caaaatctag gattgccgcg actttatcgt tttcatcaaa taaaaggttg
     1441 ccggtgcggt aatcattgtg aataaggact ggtgtgaatt tttgattttt tccccaagat
     1501 aaaatctttt ccacttcagc gacaaattct ttgccactgg atactttttc ttcaacttct
     1561 ttttgtttcg ctattactcg ttccaattct gtaaataccg tgcgtttccg agacaaagat
     1621 atgtcgtgtc gacgagaaac attgtgaagt ttggctagcg cttcagcggc tgacttaact
     1681 gccgaaagcg gtggcggttg gtcaatacca aatggaaggt gcttaccttc cacgaattca
     1741 aaaattgtca gcgtttttcc agtcggcaaa atcgcgaatg gctttttatc tttggtttct
     1801 atgatcggaa caactggcac accgtcagcc aaaagaactt ttagccactc cgcttcaaaa
     1861 gcgatatcac cacccaattc tcgcttgctc attcgagcga catatttctt gccattttta
     1921 atttccaacg tccatattat attatccgac ccatcgcgca agacactcgc tcgcgcggca
     1981 tccaatccgt aaatttcatt gcaggatttt agtagcgttt gttcgttcgt attcgtaatc
     2041 tttcgcatat ttaaatgcta ccatcaacca ccgaggttgg gaataaaaag tctagataaa
     2101 taaaaaagtt gacaagacag cctctaaatg attaaaatgc gattagtttt caacgaggcc
     2161 atactggtct tttatgaaac aagttcctta acaatagaaa ggagtgacaa aaaatgaaaa
     2221 acttgagcat tactttcgtt cctcgcttgg gcgagttgga agttgaggtg gttgaacgta
     2281 aaggtatcgg tcacccagac accattgcag acgcactggc ggaaagtttg tcagttgctt
     2341 acgcgcggta cactctcgga cacttcggcg ccatcttgca ccacaacttt gataagctgg
     2401 cggtgctcgg cggaactacc gaagtggatt ttggcacggg aaaacttaca agtcctatca
     2461 gggtaatctt gaacggacgt gcctcgatgc ggtttggtga caaagcgatt cctgttcgag
     2521 atatcctcga agcagaaact cagcggtttt tctcctcgct tttcggcgac agcctgaatg
     2581 cggagcgaga cattgagaaa cactggttcg taagtagcgc aagcggtcca ggtaaaacaa
     2641 gatggagcac cggctcacgc gctcatctct ttactccgcg aagcataagc gaagtaaagg
     2701 gatacgatcg cttgctcaac aatgacacct cgatgggttg cggctatgct ccgctttctc
     2761 cagttgaaac ggcggtactt gcgcttgaag ggaaactaaa ctcttcagac atgaagcaga
     2821 agcatctgtg gcttggcacg gacatcaaag tgatggccgt aaaaactcac gggcagtgcg
     2881 gcgtgacgat ttgtctaccg cagattgctc agtacgtgtc gtctctcgag tcctaccaag
     2941 ataaccttgc agcgatgcaa tcaattatcg aggagattct cggcgaactc ttgccaggaa
     3001 tgcgcatcgc gctttcaacg aacatgcgcg acaacattgc acacggcgat gtgtacttga
     3061 cggcgatcgg ctcttcaatc gaatcaggcg acgaaggaat tgtcggacgt ggtaatcgga
     3121 tcaacggtct tattacgcct ctacgaccga taaacctcga gggtgcgtgt ggcaaaaatc
     3181 cgaagtacta caccggcaag ctctacaatg tggcggcgca taaaatcgct catcgcctct
     3241 acggcgagac acggaaggcg tgcgaggtgc tgcttgtagg tcaaacgggc cagccagttg
     3301 tcgaaccatg gcacacgcta attcgcaccg ccgaaacacc tcttgacgaa aaacgcatca
     3361 agctaatcgc cgaagaggaa ttaacgcgca ttccgcaaat cacggaagcg ctccttgaac
     3421 gcaaagtcag actctactga aacggaggac cagatgaacg gatcaaaatc tacgattgaa
     3481 gcgacgcgcg acgaattttt cgcgcgtcgc agttggcaac ggaaacaatg tccgctttgc
     3541 caagagaact actacactaa acgc
//
LOCUS       DATDSU010000004         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1340034, whole genome shotgun sequence.
ACCESSION   DATDSU010000004 DATDSU010000000
VERSION     DATDSU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340034"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1318
                     /locus_tag="VI981_00080"
     CDS             329..1318
                     /locus_tag="VI981_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4478750.1"
                     /translation="MNKIIIAIVAVVVVGALAYFGFQRTNQSPTSGEPIKIGVVVYPS
                     GGIFYVAQEKGLFAKYGVNAQPIEVSPDAAIQLLASNQIQMLFLTSDFMPIVADAGVE
                     AKQIFSPSISYGADGMVVTKNVQSFSDLKGKKIYLGFGFPSDFLFHYVAEKNGLASSD
                     FDIVNLGPEDVGASFVSGKIDAGMTWEPWLSKTIERKDGKVLFTSKDYPGVITDIVMA
                     RTDLIQNRRTDVENVMRAFFEASDWWTKNTTAGNAIAAKAFSLTPEDFAPMRDTVKFN
                     DLQANLEKFDKSKPLNVYDIVEQASAVYRQEGVIKANVRGDSMTDSSLLNDIR"
     gene            1318..2112
                     /locus_tag="VI981_00085"
     CDS             1318..2112
                     /locus_tag="VI981_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4478751.1"
                     /translation="MKKGFYLKLDTSKGRSAILTFFGVASFLALWSLATYSGFVEPLF
                     LPSPTAVLLAIGNLFRDFNLLHDIWASVFRILIGFGLSVVIAIPLGVYLGMSKAFEAF
                     LEPIVSFIRYTPISALVPLFMLWLGIGETEKIFLIFAGVLPNLVMMIFDVVANARKEY
                     TEAAYSLGAGNRDILFSVILPQSLPGIWNAMRVNIGLAWTLIILAEIIAATSGLGHVI
                     ITSQRFLQTANVMGAMFIIGILGLVTDLLFKISYKFLFPWMAKSHA"
     gene            2105..3745
                     /locus_tag="VI981_00090"
     CDS             2105..3745
                     /locus_tag="VI981_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4478752.1"
                     /translation="MLELKNISKTYSLGARHIEAVRGLNLSIREGELVAIVGPSGCGK
                     TTMLKIIAGLLTPTDGEVLLDGKKVEGPGIEMGWVPQDYTLFPWLTVRENIAFGLRFR
                     ADKTSREKAEIVQHFINMTGLRQFAEPYPSELSGGMKQRVAIARTFANDPKLIALDEP
                     FGSLDSQTRSQLQDFLLGLWQEEKKTIILVTHDLEEAIYMADRIIILSTQPGTVKEIL
                     EINLPRPRTHVLRYEQEFGKLRKYLYYQVRSEVIKASWTSPQDSSAGYPRLGLHAWLG
                     DLPFYLAKEMRLLEKYRIPIEFLNLEKREDRTGLWLNGDLEFLNVTLDVAVLLREKRP
                     ELKILLPMSQSIGAEALIARNEIATLNDLRGKRIAIEKDWLSYFFLLYVLDSAGISPH
                     EVELVDMDVSKTGSALLAGEIDAGVLWEPWLAKTLELGTLHTLADSKKHPVIYDVLIA
                     TESVLKKYPTYVTGLRSAWHDSVEMFEKNKIDSIRKTSYYTGTSPEEVALQLGQIRFL
                     DTANMKEIVDMISTVQDAFAKAGVLHNKRYPSDFIFPL"
     gene            3925..>4246
                     /locus_tag="VI981_00095"
     CDS             3925..>4246
                     /locus_tag="VI981_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478753.1"
                     /translation="MTSNGVTADETVLLDADAAVALPIEDVPNEPRIYEIGYHIIPTV
                     KEEDIEKTVGEIRKGIESSAGKASFIAEGAPSLIKLAYPMSVREGEKYTEYDRGYFGW
                     IKFEA"
BASE COUNT         1140 a          955 c         1047 g         1104 t
ORIGIN      
        1 cacttggccc accctcccgt ccgcgtgcat aaacttccgc cgcggcagag tcaaagcgcg
       61 gcggactccc tcgccaaaca aaattctaaa tcgatgaatg ctctgccaaa aacttgacaa
      121 atggagcgat aagtgtacag tagcgaacaa gaggataggt aaagcctctc tctaaaaaac
      181 tttccatagc gatgcggcgg ctgccgtggg ctgtggaaat ttttgtaggg aacatactct
      241 gcaattaacc acaatccaaa gagccgcctt gtagcggctc tttgcgtgtt agcattatta
      301 gtaggtgtct ttagtaaaca tattctttat gaacaaaatc attatcgcca ttgtagcagt
      361 tgtcgttgtt ggggcgcttg catactttgg ttttcagaga acaaaccaat ctcccacaag
      421 tggagagccg atcaaaatcg gtgtagtcgt gtatccgagc ggcgggatat tctacgtcgc
      481 gcaagaaaaa ggacttttcg ctaagtatgg cgtgaacgcg caaccgatcg aagtttcgcc
      541 ggatgctgcg atacaacttc ttgcgagcaa tcagatacaa atgttgtttt tgacttccga
      601 cttcatgccg atagttgcgg acgcgggagt agaggcaaaa cagatattct ctccgagcat
      661 atcgtacgga gcggatggta tggtcgtaac aaagaatgtt cagagctttt ccgatttgaa
      721 aggcaagaaa atctatcttg ggttcggttt tccaagtgac tttctattcc actatgtcgc
      781 cgagaaaaac gggcttgctt cttctgactt cgatatcgta aatctcggtc ctgaagatgt
      841 cggcgcttcc tttgtatctg gcaagattga cgcgggtatg acatgggaac catggctctc
      901 aaaaacgatt gagaggaaag atggcaaagt gttattcacg agcaaggact acccgggggt
      961 cattacggac attgtcatgg ctcgaacgga cttgatacaa aaccgccgca cggacgttga
     1021 aaatgtcatg cgcgcgtttt tcgaggcgtc tgattggtgg acgaaaaata ccaccgccgg
     1081 aaatgcaatt gccgcaaaag cgttcagtct aacaccggaa gactttgcgc ccatgcgcga
     1141 taccgtcaaa ttcaacgact tgcaagcgaa tcttgagaaa ttcgacaagt caaagccact
     1201 caacgtgtat gacattgtcg aacaggcatc tgcggtctac cggcaagaag gcgtgataaa
     1261 ggcgaacgtg agaggggact ccatgacaga ctcctccctg cttaatgata ttcgctgatg
     1321 aaaaagggat tctacctgaa attagatacg tcaaaaggcc gatcagctat actgaccttt
     1381 tttggtgttg cgtctttcct cgcgctctgg tcgctggcaa cgtattctgg cttcgttgaa
     1441 ccacttttct tgcccagccc gacggctgta ttgctggcga ttgggaatct tttccgcgac
     1501 tttaatttgc tgcacgatat ttgggcaagt gtttttcgga ttctgattgg gttcgggttg
     1561 tctgtcgtta tcgctatccc actcggggtg tatttgggaa tgagcaaagc gtttgaggca
     1621 tttcttgagc caatagtcag ttttatccgg tacacgccca tttccgccct tgtcccgttg
     1681 ttcatgttgt ggcttgggat tggagagacg gaaaaaatat ttttgatttt tgcgggagtt
     1741 ctaccgaact tggtaatgat gatatttgac gtggttgcca atgctcgcaa ggaatatacc
     1801 gaggcggcat actccctcgg tgcaggaaat agggacatac ttttcagtgt cattttgccg
     1861 caatcgttgc cgggtatctg gaacgcaatg agggtcaata tcggactagc gtggacgctc
     1921 ataattttag cagaaatcat cgctgcaact tccgggcttg gccatgtcat tattacttcg
     1981 caaagatttt tacagactgc aaacgtcatg ggagccatgt ttataatcgg tattctaggc
     2041 ttagttaccg accttctctt taaaatcagc tataaatttc tgttcccgtg gatggcaaaa
     2101 tctcatgctt gaactcaaga acatctcaaa aacatattcg ctaggggctc ggcatatcga
     2161 ggcagttaga ggcctcaatc tttccatacg ggagggagaa cttgtagcaa tcgttggccc
     2221 tagcggctgt ggtaagacga caatgctaaa gataattgcg ggtctgctta cgccgactga
     2281 cggagaggtg cttttggatg ggaaaaaggt tgagggtccg ggaatagaaa tgggctgggt
     2341 tcctcaagac tacactctgt ttccgtggct caccgttcga gaaaatatcg ccttcggcct
     2401 ccgttttcgt gccgataaaa catcacgaga aaaagcagag attgtccaac attttatcaa
     2461 tatgacgggc ttacgacagt tcgcggagcc atatccgtct gagctttcgg gagggatgaa
     2521 gcaacgtgtc gctatcgcgc gcacctttgc caacgatcca aaactcatcg ccctggacga
     2581 accctttggc tcactcgact ctcagacccg ttcacaatta caggacttct tattgggact
     2641 ctggcaggaa gaaaaaaaga caataattct cgttacgcac gacctcgaag aggcgatata
     2701 catggcagat cggataatca tattatcaac acagccaggc acagtaaaag aaattttaga
     2761 aatcaattta ccgcggccgc gaactcatgt tttaagatac gagcaagaat tcggaaagtt
     2821 gcgtaagtat ctttactatc aggtacgttc ggaggtaata aaagcctctt ggacttcgcc
     2881 ccaggattct tcggctggat acccgcgatt ggggctccat gcatggctcg gtgatcttcc
     2941 tttttattta gccaaggaaa tgcgcctgtt ggaaaaatac agaatcccca tagaattttt
     3001 gaatcttgaa aagcgcgaag atcgcaccgg actatggctt aatggagatt tggaattttt
     3061 gaatgtgact ctcgatgtag ccgtactctt acgagaaaaa aggccggaac tcaaaatact
     3121 gctgcccatg tcccaatcca tcggagcaga agcgctgatt gcccggaatg aaattgcgac
     3181 tctaaacgac ctgcgaggta aaagaattgc tatagaaaaa gactggctta gttatttttt
     3241 tcttctgtat gttcttgatt cggccggaat ttcccctcac gaggttgagc ttgtcgatat
     3301 ggatgtgagt aaaaccggaa gcgcacttct cgcgggcgag atagacgcag gggttctttg
     3361 ggagccgtgg ctagcgaaaa cactcgaact tggcacactt catacccttg ccgactcgaa
     3421 gaagcatccc gttatttacg acgtattgat tgccacggag tccgtgctta aaaaatatcc
     3481 cacgtatgtt acaggattgc gttcggcatg gcatgattcc gtggagatgt tcgaaaaaaa
     3541 caagattgat tcaattcgaa aaacttctta ttacacagga acttcgccag aagaagtcgc
     3601 gctccaactt gggcagatac gttttttgga caccgcaaat atgaaggaaa tagtcgatat
     3661 gatatcaact gtgcaggatg cttttgcaaa agcgggcgtc ctgcataaca aaagatatcc
     3721 gagcgacttc attttcccac tgtaaagcat ccagtacaca atgcggtaac actgtctttt
     3781 tttatctcgc gcctattgcc tagtgcccac tgcctatgct acagtgcccg ctacacctgc
     3841 cccgttagaa gtccagctaa cgccgatttc aatttgacga ggatgtgcta aacaaaccga
     3901 acaaaaaaca tactatatat gacgatgact tctaacgggg tgactgcaga cgaaacagta
     3961 cttttggatg cggacgcggc cgtcgctttg cctattgaag atgttccgaa cgagccgcgc
     4021 atatacgaaa tcggatatca cattattccg acggtaaaag aggaggacat tgaaaagacg
     4081 gtaggtgaaa tccgcaaagg cattgaatct tcggccggaa aagcttcttt tatcgcggaa
     4141 ggggcgccgt cgttgataaa acttgcgtat ccgatgtcag tacgggaagg ggagaagtac
     4201 acagagtatg accgcgggta tttcggatgg ataaagtttg aggccc
//
LOCUS       DATDSU010000005         4658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1173531, whole genome shotgun sequence.
ACCESSION   DATDSU010000005 DATDSU010000000
VERSION     DATDSU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173531"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VI981_00100"
     CDS             <1..185
                     /locus_tag="VI981_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478754.1"
                     /translation="KVRGAYLSMERNLPYLFTYQKYPELNIPNTTNSLDGSFTHLKDK
                     VNRIFITDCAKTGDTK"
     gene            complement(252..803)
                     /locus_tag="VI981_00105"
     CDS             complement(252..803)
                     /locus_tag="VI981_00105"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478755.1"
                     /translation="MEKKSKKRKQRQNLQNIILSSVAAAGLLSVALVAPNVVKLLDGF
                     VGKNKTSIHRARRRLVEAGLLAWGGNGRLRVTPRGEAKLRQLEVANYKIKKPKRWDSR
                     WRMLVFDISEKRKTLRDKVRRTLQAIGFFKLQDSVWVYPYDCEDLIVLMKSDFKIGED
                     LLYVIADTIENDMKLRMFFSLKN"
     gene            complement(933..2684)
                     /locus_tag="VI981_00110"
     CDS             complement(933..2684)
                     /locus_tag="VI981_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY4478756.1"
                     /translation="MSITKRVGENIRNHREMKNFSQGELARRLRVSRSYISTLENGAR
                     NPSIKSLEKIARVLEVNVASLVGESKFGEANSHVMPYVAGLIKLKGQEWNGKKWYHQR
                     FDGAPYLIHMIAEAQILTRRNQKPGLNFNKHFCFFEDGKADWYMDTQEIEHITEHMTE
                     LGEKRPNFSKELMKEYEPFEKAFYSECEKIEKQNLYKLNNQNLIEIHDAFLESILKRN
                     SSSSIIDGFALGSDRILEDSIRKAYESNPKISKRMSFQEVFSTLTTASHLSFVREAEI
                     ELFKLILAVRECGSNRRKLISEYRDRYFWITNNYVDAHVLSIPYFEDEMASIEASGVD
                     VAAQIIALENSPSINAKKKEVLLKEMNLDKRTLFLLSVTEDFTAWQDERKKATLFTAH
                     YCTLLLDEISRRVGISSNLLKYLSPREVSEIFTDTPPASALEARKTGAVFYWDESGHE
                     ARVGEEVKKIREELFGNRKLDDVQDFRGMTASLGKVQGHVRILKSVKEINSIKKGEIL
                     VAVMTRPDYVPAMKKAAAIITDEGGITSHAAIVSRELKIPCIIGTKIATHVLKDGDLV
                     EVNANHGSVKIISRVAR"
     gene            complement(2804..3571)
                     /locus_tag="VI981_00115"
     CDS             complement(2804..3571)
                     /locus_tag="VI981_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478757.1"
                     /translation="MSEYKWELIQPSDDLWQDSIRCQALYECPKDVAGKRLGPLVPYA
                     GKYGSGKNFVGEVYFNFGWVEPHVRVVEAFAVNIAKQIAQNPAFAAASTIVGIPEGGR
                     TLAQEVARHSGKRFVYPLKEPKGEQKTGGKAEFNFVFTRNDLHPGEAVIVVDEVHNNF
                     QNTTDTIETIAETGATVVGFCSALNRSETVEGFFCPKTGAFASDNFPLISAIRRPLKE
                     FIQEHPDVAQDIGKGNIEWKVKQNWARLRASMEAHRK"
     gene            complement(3568..4389)
                     /gene="pyrF"
                     /locus_tag="VI981_00120"
     CDS             complement(3568..4389)
                     /gene="pyrF"
                     /locus_tag="VI981_00120"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY4478758.1"
                     /translation="MGEPRNRKEQRQKQKTRKLMNTQTTSRIILALDVDSVEKAVQLM
                     DATKDHIGMVKIGLELINAQLAGEVAKAAHARSVGIFWDGKWDDIPTTAGNAMKVTAQ
                     MPGICMVNVHVNADIEGMMAVVANKGNALVLAVTELTSLDTESVELKAGASRNASVLY
                     DARMAAYAGCDGVICSPKELSLLGRYANRKINPELPLTKLIKVVPGTRSLGGDAQDQK
                     NVDSQESAILNGADWLVIGREVTKNPNMSPAEAARALNVRMAIAFAEREKGAVAK"
BASE COUNT         1171 a         1143 c          982 g         1362 t
ORIGIN      
        1 agaaagtcag aggagcctat ctcagcatgg agagaaacct gccctacttg tttacctatc
       61 aaaaatatcc tgagcttaat attccaaaca ccaccaactc gcttgatggg tcttttaccc
      121 atttaaaaga caaggtgaat agaatattca tcacggattg cgccaagaca ggagatacaa
      181 aatgattgaa gagatactaa gaggaaagga agattagcaa cccaaaaggg gtattagacc
      241 actaattcta actaattctt gagactgaaa aacattctga gtttcatatc attttcaata
      301 gtgtcagcaa taacataaag taaatcctcg ccgattttga aatctgattt cattagaaca
      361 attagatctt cgcagtcata cggatacacc caaacgctat cctgaagttt aaaaaaaccg
      421 atcgcttgaa gtgttcgtct tactttatcg cgtaaggttt tgcgtttctc gctaatatca
      481 aaaacaagca ttcgccacct tgaatcccag cgttttggct ttttaatttt gtagtttgca
      541 acttccaatt gtctcaattt ggcttcccct ctcggagtaa cccttagacg gccattacca
      601 ccccaagcca atagtccagc ttcaacaagt cttcgtcgcg ctctgtgaat gctggttttg
      661 ttcttaccca caaacccgtc aagtaacttc acgacattag gcgcgacgag agcgacagaa
      721 agtaacccag ccgcggcgac gctcgataaa attatgttct gaagattttg cctttgcttc
      781 cttttttttg atttcttctc catttctccc atagtatact aagtatacgc tatagcgtat
      841 tgaatgtata ctacgtcatc cagatgctta gaatcaactc tattgcttaa ttatttaata
      901 taagagaaaa gcaagaaata tttttgtaat tgttatcgag caacacgact gataattttt
      961 actgaaccgt gatttgcatt aacctctaca agatcaccgt ctttaagcac atgagtggca
     1021 atcttcgtac caataatgca gggaatttta agttcacggg agacaatagc agcgtgagag
     1081 gtaatccccc cttcatcagt tattatggct gcagctttct tcattgccgg aacatagtcg
     1141 ggtcgcgtca taacagccac gagaatttcg ccttttttta tcgaattgat ttctttcaca
     1201 ctctttagta tacggacgtg tccctgaacc tttccgagtg aagctgtcat tccacggaaa
     1261 tcctgaacat catctagttt acgattgcca aatagttctt cgcgaatttt cttaacttct
     1321 tccccaactc tcgcctcatg tccgctttca tcccaataaa atactgcgcc cgttttccgc
     1381 gcttcaagcg ctgatgcagg gggagtatcg gtaaatattt ccgacacttc ccggggagaa
     1441 aggtatttta aaagattgct cgaaattcct acccgcctag aaatctcgtc gagcagcagt
     1501 gtgcaatagt gtgcagtgaa aagagtggct ttctttcgct cgtcctgcca tgcggtgaaa
     1561 tcttctgtga ccgacaagag aaacaaagtt cttttgtcta aattcatttc tttcaataac
     1621 acttccttct tttttgcgtt tatagatgga ctattttcca aggcgatgat ttgcgccgcg
     1681 acgtccacac ccgaagcttc aatactggcc atctcatcct caaaatatgg aatagagaga
     1741 acgtgggcat caacataatt atttgttatc caaaaataac ggtctcgata ctcacttatc
     1801 aattttctcc gattggatcc gcactcacgc actgccagga ttaatttaaa caactctatt
     1861 tcagcctctc tcacaaacga caggtgagac gccgtggtaa gtgttgaaaa aacttcctgg
     1921 aaactcatgc gtttactgat ttttggatta ctctcgtacg cttttcttat gctatcctcc
     1981 agaattcggt cactgccaag ggcaaatccg tcgataatag aacttgaaga attccttttt
     2041 agaattgact ctagaaaagc atcgtgtatc tcaatgagat tctggttatt caatttatat
     2101 agattttgtt tctctatctt ttcacattca ctgtaaaaag ctttctcaaa tggctcatat
     2161 tctttcataa gttccttact gaaattcggg cgtttctcac caagttccgt catgtgctcc
     2221 gtaatatgtt caatttcttg agtatccata taccagtcag ccttaccgtc ctcaaaaaaa
     2281 caaaaatgct tgttaaagtt gaggccgggt ttttgattgc ggcgggttaa tatttgagcc
     2341 tcggctatca tgtggatcaa atacggtgcg ccgtcaaaac gctggtgata ccattttttt
     2401 ccgttccatt cctgtccctt cagtttgata agccccgcga catacggcat cacatgagag
     2461 ttagcttctc caaatttaga ctcaccgaca agagatgcga catttacctc gagcactctc
     2521 gcaattttct ccaatgattt gatggatggg ttacgagcac cgttttcaag cgtgcttata
     2581 tatgagcgac tgactcgaag tctgcgcgcc aactcccctt gggagaaatt cttcatttcc
     2641 cgatgattac ggatattttc ccccactctc ttggtaatac tcatgggcgg tatggtaacc
     2701 ctaatcacat tttgagtcaa cggttactta tgagtcacta ctgtatgggt ataaaaaacg
     2761 gcgttgctgt gccgtttgga catgaactaa aagtatgatc aggtcatttt cgatgtgctt
     2821 ccatcgacgc gcgcagcctt gcccagttct gcttgacctt ccactcgatg tttcccttgc
     2881 cgatatcttg ggcaacatca ggatgttctt gtatgaactc cttaagtggc cgacgaatgg
     2941 ccgagatgag tggaaagttg tctgacgcaa aggctccagt tttggggcag aaaaaccctt
     3001 cgacagtctc ggaccgattg agcgccgagc agaagcctac caccgtcgca ccagtctcgg
     3061 cgatcgtttc gatcgtgtca gtggtgtttt ggaagttgtt gtgaacttca tcaacaacaa
     3121 tgacagcctc gcctggatga agatcgttgc gggtgaagac aaaattgaat tcagccttgc
     3181 cgcctgtctt ctgttcccct ttgggctcct taagcggata gacgaaacgc ttaccgctgt
     3241 gacgcgccac ttcctgtgcg agtgtccgtc caccttcagg aataccgacg atcgttgaag
     3301 ccgctgcgaa ggcaggattt tgtgcgattt gcttcgcgat gttcaccgcg aatgcctcca
     3361 caactcgtac atgaggctca acccaaccga agttgaaata cacctcgccc acaaagtttt
     3421 tgccgctgcc gtattttccg gcatagggca cgagggggcc gagtcgtttg ccagctacgt
     3481 ctttcggaca ttcgtagagg gcctggcatc gaatactgtc ctgccagagg tcatcacttg
     3541 gctgtattag ttcccatttg tactcgctca tttcgctacc gctcctttct cacgctccgc
     3601 aaaagcgatt gccatgcgca cattgagtgc ccgcgccgct tctgccggtg acatgtttgg
     3661 gtttttggtg acttcgcgac cgatgacgag ccagtcggcg ccgtttagaa ttgctgactc
     3721 ttgcgagtcc acgttttttt ggtcttgagc gtcaccaccg agcgaacgag taccgggcac
     3781 aaccttgatg agctttgtga gcggcaattc tggattgatt ttacgatttg catacctccc
     3841 cagaagcgaa agttctttgg gagaacaaat gacgccgtca cagcctgcat acgcagccat
     3901 gcgtgcgtcg taaagtacgc tcgcgtttcg agacgctccg gctttgagtt ccacactctc
     3961 cgtgtcgaga ctggtgagtt cagtgactgc gagcacgagc gcgttaccct tgttggccac
     4021 aaccgccatc atgccttcga tatctgcatt cacgtgtacg ttgaccatac aaatgcccgg
     4081 catttgtgcc gtaaccttca tggcgtttcc cgctgtggta gggatgtcgt cccatttacc
     4141 gtcccagaaa atgccgacac tgcgggcatg ggccgccttt gccacctccc cggccaattg
     4201 agcgttgatg agttcgagtc caatcttgac catcccaatg tgatcctttg tagcgtccat
     4261 gagttggact gctttctcaa cagaatcgac atcgagggcc agaattatcc gactggtcgt
     4321 ttgcgtgttc atgagtttcc gtgttttttg tttttgccgt tgttctttcc gatttctcgg
     4381 ttcacccatt cgggtttctg tttctgtgcc taatactata cgcaaaactg aaaaaaacaa
     4441 gtcgaaaaat gttatacact cggtgtttaa ccgtaaagct atacgaaaaa cagccttact
     4501 taaaggctat tttctccaag gtccgacctt ggataatact ggccgagaat agtgatccgc
     4561 cagcaggcgg atatgcgcgc attttacgtt agagcgagca taaccttctt aaaatgccgg
     4621 gggcgctaca gtgtccgtat caagcattca agcgaggg
//
LOCUS       DATDSU010000006         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1508582, whole genome shotgun sequence.
ACCESSION   DATDSU010000006 DATDSU010000000
VERSION     DATDSU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508582"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VI981_00125"
     CDS             <1..604
                     /locus_tag="VI981_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY4478759.1"
                     /translation="AQMSRAVGIHLMLSTQRPSVNVITGLIKANISSRIALQVTSQID
                     SRTILDTGGAEKLLGAGDMLFLSGEMSKPQRIQSAFISENELKKVVKFLADNYANELP
                     SEINLTSPDKNFIFESTVSDDASLEDDDILYEEAKQTVVEAGKASTSYIQRKLRVGYA
                     RAARLMDLLEERGVIGPADGAKPRSITGVETNTGEGAEQI"
     gene            609..980
                     /locus_tag="VI981_00130"
     CDS             609..980
                     /locus_tag="VI981_00130"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478760.1"
                     /translation="MNRDHKHLISLLLGVFLVTAIVALGIYETRDIIKGPGITVEYPT
                     RGELIVNPLIEVQGKVKNASGVYLNGRKIFITEQGRFKEKLLLLPGYNIITFTVEDRL
                     KRKKEARVEVVFDNSGAPDSL"
     gene            1089..2120
                     /gene="recA"
                     /locus_tag="VI981_00135"
     CDS             1089..2120
                     /gene="recA"
                     /locus_tag="VI981_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY4478761.1"
                     /translation="MAKNKLVKEAGTPIAEKARPSIEDTLNEIKSKFGEEAIMKLGDK
                     PRVHVNAISTGSIGLDAALGVGGIPRGRIIEIFGPESSGKTTLSLHVIAEAQKTGGIC
                     AFIDAEHAMDPEYSKKIGVKIDELLISQPDNGEQALEIVESLVRSGKMDVIVIDSVAA
                     LTPKDEIEGDMGAYHVGKQARLMSQALRKLTAIVAKSKTIVIFINQIRMQIGVMFGNP
                     ETTPGGKALKFYTSVRLDIRRIAQIKKGEEVMGGRVRVKVVKNKVAAPFKQTEFDLMY
                     NEGISKEGEIIALGEKMGLITKSGTSYSHGEEKLGRGYDATRQFLKDNPKISGDILKE
                     IRKKLAEAI"
     gene            2225..2845
                     /locus_tag="VI981_00140"
     CDS             2225..2845
                     /locus_tag="VI981_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478762.1"
                     /translation="MAIFKGSPLLAKKISHMAILVYNEIRERRYIIMDNEPYEVLTAH
                     VFRKQQRKPVNATKLRNLITGSVVEHSFAVSDKVEEAELESRKIKYLFSNKGEFWFCE
                     EKDPSKRFSMKEEIVGSKGKFLKQNTILDILTFQDRLVGLKLPVKVDLRVIEAVNAVK
                     GNTVSGGSKAVKLETGAEIYVPMFINEGDVLRINTETEEYTERVTK"
BASE COUNT         1037 a          651 c          724 g          796 t
ORIGIN      
        1 cgcccaaatg tcccgagcgg taggtattca tttgatgctt agcacgcaac gaccttcagt
       61 aaatgttatc accgggctca ttaaagctaa catttcttct cgaatcgccc ttcaagttac
      121 ctcccaaatc gattcgcgaa cgattcttga taccggcggc gctgaaaaat tacttggcgc
      181 cggcgatatg ctcttcctgt cgggagaaat gtcaaaacca cagcgtattc aatcagcctt
      241 tatttctgaa aacgaattaa aaaaggtcgt gaagttcctg gccgacaact acgcaaacga
      301 gctgccaagc gagattaacc ttacgtcccc tgacaaaaac ttcatatttg agtcaactgt
      361 tagcgacgac gcgtcgcttg aggacgatga catactttat gaagaagcga aacaaactgt
      421 ggtagaagcg ggaaaagcgt cgacatcata catccaaaga aagttgcggg ttggatacgc
      481 tcgagcggca cggcttatgg acttacttga agagcgaggc gttatcgggc cagcagacgg
      541 cgctaagcct cgttccatca caggagtaga gaccaacaca ggagagggcg ctgaacaaat
      601 atagcctgat gaatcgagac cacaaacatc tgataagcct gctactcggt gtttttttgg
      661 tgacggcgat agtagcgctc ggaatatacg agacgcgaga tatcattaaa ggaccgggaa
      721 tcactgttga atatccgacg cgtggggaat tgatcgttaa tccgcttatt gaggttcaag
      781 gtaaggtcaa aaacgcgtca ggtgtttact taaacggtag gaaaattttt atcacagagc
      841 agggacgatt caaggaaaaa ttgctcctcc tgccaggcta taatataata acctttactg
      901 ttgaggatcg tctcaaacga aaaaaagaag cgcgtgtcga agtagtattc gataatagcg
      961 gtgctccgga ttcattataa aaatactaat taacgaatga gtactaatat acaaataata
     1021 ctaatgcaac ttattcgtat tcattagtat aattggtata aattagtata ttagtattac
     1081 aaatttgtat ggcaaaaaat aaactcgtaa aggaagcggg aacaccgatc gcggaaaaag
     1141 cgcgccctag catcgaagat acactaaacg aaatcaagtc caaattcggt gaagaagcaa
     1201 tcatgaaact tggcgacaag ccgcgtgtac acgtaaatgc catttccacg ggttcgatcg
     1261 gtctagatgc ggcgctcggc gttggtggaa tacctcgagg aagaataatc gaaatcttcg
     1321 gaccggaatc gtctggaaaa acaactctat ctttacatgt aattgccgag gctcaaaaaa
     1381 cgggggggat atgtgcgttt atagacgcag aacatgccat ggatccagaa tactcaaaga
     1441 aaatcggtgt caaaattgac gaacttctaa tttctcaacc agacaatggt gaacaagctc
     1501 ttgagatcgt tgaatcactc gtgcgttcgg ggaaaatgga cgtcatcgtc atagactccg
     1561 tggctgcact tactccgaaa gacgagattg agggcgatat gggagcatac catgttggca
     1621 aacaggcacg gctcatgtca caggccttgc gaaaacttac agccatcgtc gccaagtcaa
     1681 aaaccattgt tattttcata aaccagattc gcatgcaaat cggcgtcatg tttggaaatc
     1741 ctgaaacaac gccgggcgga aaagccctga aattttacac gtcagttcgt ctcgatattc
     1801 gtcgcattgc ccaaattaaa aaaggagaag aagtcatggg aggccgcgta cgagttaaag
     1861 tagtaaagaa caaagttgct gcaccgttta aacagaccga atttgatctc atgtacaatg
     1921 aaggcatctc caaagaggga gagatcatcg cccttggaga gaaaatgggg cttattacaa
     1981 aatcaggcac atcctacagc cacggagagg aaaaacttgg ccgcggctac gacgccacta
     2041 gacaattctt gaaagacaat ccaaaaattt caggcgacat tctgaaggaa atccgaaaaa
     2101 aactagcaga ggcgatttag gttttaaggg ctacccagcc ccaatttgag aaattcagct
     2161 ttacttagtt ttttgagtct gatacattac atcagcaatt attttattaa tgcattgagt
     2221 cgggatggcg attttcaaag gctcgcccct gctcgctaag aaaatcagcc acatggcaat
     2281 ccttgtatac aacgaaataa gagaaaggcg ctacataatc atggacaatg agccctatga
     2341 agtgcttaca gcacatgtct ttcgcaaaca acagcgaaaa ccggtaaatg ccacaaagtt
     2401 gcgcaacctc atcacgggaa gtgttgttga gcactctttt gccgtatctg ataaagtcga
     2461 ggaagctgaa cttgagtcaa gaaaaatcaa ataccttttt agcaacaagg gtgagttttg
     2521 gttctgtgaa gagaaagatc cgagcaagcg tttttcgatg aaagaagaaa ttgtcggatc
     2581 gaagggcaaa tttctaaaac aaaacacgat cctggatatt cttacattcc aagatcgact
     2641 tgttggtctc aaacttccgg ttaaagttga ccttagggtt atcgaagctg taaacgcagt
     2701 aaagggcaat acagtttcag gcggatcaaa agccgtaaaa ctagaaacag gagcggaaat
     2761 atacgtgcca atgttcatta acgagggcga tgtacttcgc atcaatactg aaactgaaga
     2821 atacacagag cgagtcacga aataattata atattaattt tctagaattt cactaataac
     2881 ctcttctggc gcacattctg gtttttctgc gacaagagag attcagggta attgtctcag
     2941 ggagtcgtgt tacagtttaa aggttaaaat ttagtataca ttcaatacgc tatagcgtat
     3001 tgaatgtata ctgtataaac taatccgagc taaaggcctt tttgggttgc tacttttgat
     3061 atactgtggc tctactaatt gatacttttc ataaaatata caaacatgtt taaaaaaatg
     3121 gaagaatttg tcccaaatgc aggtcgtctc atgtaaagaa atatggtgtg cgaagaggtt
     3181 tgcagcgata tcgatgcgta tcatgcct
//
LOCUS       DATDSU010000007         7639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1007729, whole genome shotgun sequence.
ACCESSION   DATDSU010000007 DATDSU010000000
VERSION     DATDSU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007729"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VI981_00145"
     CDS             <1..183
                     /locus_tag="VI981_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020053284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY4478763.1"
                     /translation="GFAPGIDRIVMLLMGEPNIREVIAFPKTGEGKDLMMNAPSEVSA
                     AQLQELGLRLAKEDKK"
     gene            183..917
                     /locus_tag="VI981_00150"
     CDS             183..917
                     /locus_tag="VI981_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY4478764.1"
                     /translation="MVSDEQFKVKTAVFQGPLELLLDLIEKRKLHISDISLAKVTDDY
                     IAHIQQFEHYPMADSANFILVASTLLLIKSKSLLPSLALSEEEQHSIEDLEYRLKIYK
                     EIRELAVYVKERFGKELIFQKSETKMFDPVFSPHESMKADNLFSAIQDVIKNFPRPEV
                     LPKAIVKKVISLEEMIINLTQRIQTNLKMSFREFSKHGKDEKVNVIVGFLAMLELVKQ
                     GIISVTQDKHFEDIHMETENISVPKY"
     gene            993..1307
                     /locus_tag="VI981_00155"
     CDS             993..1307
                     /locus_tag="VI981_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478765.1"
                     /translation="MKNRKPRGDWTLPARALEALPHTANNEQPRRNDSTPVSAHREFA
                     ISIAADAQANTVLACILNEDLVSRVLVNPNAATLEENRELLTPVHDAPRLVSVVHGVS
                     LG"
     gene            1465..2034
                     /locus_tag="VI981_00160"
     CDS             1465..2034
                     /locus_tag="VI981_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY4478766.1"
                     /translation="MTLAARIESLLFWKGEPVTVAWLAKTLEKTVEEINTSLGELEVS
                     LSGRGVVLVRKEDEVTLGTTKDMSAVIEKITREELARDLGKAGLETLSIILYRGPLSR
                     KDIDYIRGVNSAFILRNLQIRGLVERIQSEKDSRVMLYKATMDLLTFMGLTKIADLPE
                     YGKVRELIAAFEIESKQDNEKEEAPIDNT"
     gene            2038..2973
                     /locus_tag="VI981_00165"
     CDS             2038..2973
                     /locus_tag="VI981_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478767.1"
                     /translation="MKFGFSELLVILFLVVIGTFHFSRAENLPLARTEEAIPVVDTFR
                     DLDLEARGIFVYDLLLKKPLYEKMADTPLPLASLTKLVTVITAADILPKNAIVTIARK
                     HLKEEGDQGLFADEKWNLKNIFDFSLVSSSNDGIAAIVDTARAVISSVDSNANGARSF
                     VDFMNEKSKKLGLTSMRFINETGLDVESFDQTGGYGSARDVAMLMTWIVENEPELLEA
                     TPYERLTLVSESQKVHNIQNTNEIIGHIPSLIGSKTGYTDRAGGNLVIVFDMGFAHPI
                     VVSVLGSSIEGRFSDAEKLIAATFLYFEKNPELTF"
     gene            3159..4415
                     /locus_tag="VI981_00170"
     CDS             3159..4415
                     /locus_tag="VI981_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEY4478768.1"
                     /translation="MKQSILFSKTRKESPKDEVSKNADLLIRAGYIHKEMAGVYTFLP
                     LGLRVLSNINEIVRQEMNKIGGQEMFLSALQERTIWEKTGRWSDQSVDIWFKTKLKND
                     TVLGLGFTHEEPLTNLMRDHIRSWRDLPRYVYQIQTKFRNEIRAKSGIIRGREFLMKD
                     LYSFCRDQKEHDAFYEIVKDAYMKIFARAGIADKTYITFASGGSFSKYSHEFQTLTSA
                     GEDTIHLCLNCKIAINDEIIKENPACPRCGNRKLKREKSIEVGNIFSLGTRFSEAFGL
                     VYVDEKGAKIPVIMGSYGIGPGRLMGVIAELLSDEKGLIWPEEVAPYEIHLIAINDKT
                     GAVVGLGEKYEAELEEKNISVLFDDRDARAGEKFADADLIGIGRQVIVSEKNSAAGIV
                     EIKSRMTGTAEKKKWETFVQEITHEK"
     gene            4405..5469
                     /locus_tag="VI981_00175"
     CDS             4405..5469
                     /locus_tag="VI981_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY4478769.1"
                     /translation="MKNDTIEKIKRYLSNDIGIDLGTANTLVYLRGRGIVINEPSVVA
                     VNQKTGRVVAVGMQAKQMLGRTPAHIRAVKPLVDGVISDFEVTEEMVAYLLNKAEKNS
                     RKFLGPRVVVGVPYGITNVETRAVRDATKNSGARDVYIVEEPMAAALGIRLPVKEPVG
                     SMIIDIGGGTCDIAVISLGGIVRAKTLRIAGDKLNDDIISYVRSEFKILIGEKTAETI
                     KMTIGSVMPLGSPLECPVKGRDLVTGLPREVILTDSDIREAIALSIDTIIESVKEVLE
                     TTPPEIVSDVMHRGVHVVGGGALLRGIGALLNEAVRIPIYIADDPLTAVARGAGIILE
                     DIESYRDILIQNEDDLPHQK"
     gene            5447..6232
                     /gene="mreC"
                     /locus_tag="VI981_00180"
     CDS             5447..6232
                     /gene="mreC"
                     /locus_tag="VI981_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY4478770.1"
                     /translation="MTFHIKNRQKRFSYLVAIIALLIVLLAWIRPSFLGGFTHQIGRP
                     IWASARGVRALTRTITIGFSAKEKLAAENLRLKRELQGIETIIIRSESLERENEALRA
                     LTGKKPANTFLFARTLTKVGFSPYDTFVLNVGERDGLMKKGGLYTLEGLLVGYVDQVY
                     DHTSVARLYSSPGEEFRVLVGTEELPGTAIGRGGGNFELKLPRDLKVKKGDHIMAEDD
                     KHGVAGVVEDIELDVTNSLQTILFRQPVNIYTITEVLVEKNIN"
     gene            6234..6497
                     /locus_tag="VI981_00185"
     CDS             6234..6497
                     /locus_tag="VI981_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478771.1"
                     /translation="MKLRFFCTILLLVLIFLSPWWLTFAVALALIFYFRRYYEAFFAA
                     FLFDLLYNTSRAHLFFFAGLATVAVSFVLIEWLKKRLSLYEAI"
     gene            6502..>7639
                     /locus_tag="VI981_00190"
     CDS             6502..>7639
                     /locus_tag="VI981_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY4478772.1"
                     /translation="MWFYKREKKSTREIEPDEIFLDSHNLPEFDVHQFEGRLEKPIAI
                     RTVFIVAVFFIAIGLAYASRLWMLQIAHGEAYAERSEKNRLRHKIFFSERGVVYDRND
                     ILLARNVPNDDTPDFASRVYSDEPGLAHVLGYVKYPALDKSGFYYSLEYSGVAGVEKL
                     YDDELRGKNGLKIIETDALGEIISENIIEPPEPGKNVTLSIDSEVQSELARIIKNLSN
                     EVGFEGGAGALMDLKTGELLALTSIPEFSSDVMSEGEDEVKIQEYLESSQKLFLNRIT
                     GGLYTPGSIVKLFIALGALKEKIITPEHEILSTGSISIPNPYDSKKESIFKDWRAHGL
                     VDMRRAIAVSSNVYFFEVGGGYKDQKGLGIEKIGEYMNLFGFGRP"
BASE COUNT         2173 a         1630 c         1873 g         1963 t
ORIGIN      
        1 ggcttcgcgc ccggcattga ccgcattgtc atgcttctca tgggagagcc aaatatccgc
       61 gaagtcatcg catttccaaa aacaggagag ggaaaagacc tcatgatgaa cgcaccatca
      121 gaagtttccg ccgcgcaact acaagagctc ggcttaaggc ttgctaaaga agataaaaaa
      181 taatggtcag tgatgaacaa ttcaaagtaa aaacagctgt ttttcagggg ccacttgagc
      241 ttttgctcga tcttattgaa aaacgcaagc ttcacattag cgacatttca ctcgccaaag
      301 ttacggacga ctatattgcc cacatacagc aattcgaaca ctatccaatg gccgacagcg
      361 ctaattttat tcttgttgcc tcaacactgc ttctcatcaa gtcgaagtca ctcttgccga
      421 gtctcgcact ctccgaagaa gagcaacatt cgatcgagga tctcgaatat cggctgaaaa
      481 tttacaaaga aatcagagag ttagccgtgt atgtcaaaga acgtttcgga aaggagctta
      541 ttttccaaaa atcagaaact aaaatgtttg accctgtgtt ttcgcctcac gaaagcatga
      601 aggccgacaa tctatttagt gccatacaag acgtaataaa aaatttcccg cggccggagg
      661 tattgccgaa ggctattgta aaaaaagtca ttagtctcga ggagatgatc atcaatctca
      721 ctcagagaat tcaaacgaat ctcaaaatga gttttaggga attttcaaaa cacggaaagg
      781 acgaaaaggt aaatgtcatc gtggggtttc tcgccatgct tgagctcgtg aagcagggaa
      841 taatcagtgt gacgcaagat aagcatttcg aagatattca catggagacg gagaatattt
      901 ccgtacctaa atactgaatg tcaagcatac ttggaagcct tacttggaag ccttacttgg
      961 aagccttgct tccaagtgga ggttaagctt ctgtgaaaaa cagaaagccg agaggggatt
     1021 ggaccctacc ggctcgtgcg cttgaagcgc taccgcatac cgctaacaac gaacagccac
     1081 ggcgaaacga ctcgacccct gtgagtgctc acagagagtt cgccatttcc attgctgccg
     1141 atgcgcaggc aaacaccgtt cttgcctgta ttctcaacga agatctcgtc agtcgggttc
     1201 ttgtaaaccc aaatgccgcc accctcgaag agaatcggga attgctcact cccgtgcatg
     1261 atgcgccgcg gttggtttcg gtggttcatg gtgtttccct tggttgaagg ttgttgttga
     1321 ttattcttag tctcaacctt tccaactttc tggaccccat gattcaccga gctgtcatgt
     1381 atatgacata tagacctact ccgtgtcaag atcacgctcc aaactctata tttagagcct
     1441 ttctctgtgt tatactctcg aaatatgact ctagcggcta gaatcgaatc gcttttattt
     1501 tggaaagggg aaccagttac cgtggcatgg cttgcaaaga cgcttgaaaa aaccgtcgag
     1561 gaaataaata cgagcctcgg tgaattagaa gtatcactct ctggtcgggg agttgtgctc
     1621 gtgcggaagg aagacgaagt aacactcggc acgacgaaag acatgagcgc agtgatcgaa
     1681 aaaattacga gagaggagct cgccagagac ttaggaaaag caggactcga aacgctttca
     1741 atcattctct accgcggtcc gctctcacga aaagacatag attacatccg cggcgttaat
     1801 tcggcattta ttttgcgaaa tttgcagata cgcggacttg tcgagcggat acagtcggag
     1861 aaggacagtc gtgtcatgct ctataaagcg accatggacc tcctcacatt catgggcctt
     1921 acaaaaatcg ccgacttacc ggaatatgga aaagtacgcg agctaattgc cgccttcgaa
     1981 attgaatcaa agcaagacaa tgaaaaagag gaagccccaa ttgataacac ataacctatg
     2041 aaattcggtt ttagcgaatt attagtaatt ttatttctcg ttgtcatagg gacatttcat
     2101 ttttctcgtg cggaaaattt gcctcttgcg cgcacagagg aagccatacc ggtagttgat
     2161 acgtttcggg acttagacct cgaagcccgt gggatttttg tgtatgatct tctcttaaag
     2221 aaaccgctct atgaaaaaat ggcagatacg cccttgccgc ttgcgtctct cactaaactg
     2281 gtaaccgtca ttactgcggc cgatattttg cctaaaaacg cgattgtcac gattgcacgc
     2341 aagcatctta aggaggaagg ggatcaaggt cttttcgcgg atgagaagtg gaatctcaaa
     2401 aatatttttg atttttctct cgtctcatcg tcaaacgatg gcattgctgc aatcgtggat
     2461 acggcgcgcg ccgtgatttc atcggtggac tcgaacgcca acggcgcgcg gagttttgtt
     2521 gattttatga atgagaagtc aaaaaagctc ggtctcacct ctatgcggtt tattaatgag
     2581 acgggacttg acgttgaaag ctttgaccaa actggcggct acggttctgc tcgagatgtt
     2641 gccatgctta tgacgtggat cgtcgaaaac gaaccggaac ttctcgaggc cacgccatac
     2701 gagcgtctca cgcttgtatc ggagagccaa aaggttcaca acatccaaaa tacaaatgaa
     2761 attatcgggc atattccgtc acttatagga tctaaaacgg gctatacaga tcgcgcggga
     2821 ggaaatttag tgatcgtatt tgatatgggg tttgcccatc cgattgtcgt ctcggtgctc
     2881 ggctcctcga ttgaggggcg cttctcggac gcagaaaaac tcattgccgc cacgtttcta
     2941 tacttcgaaa aaaatcctga acttactttt tgattgaaac aacagaaaat tgccgtacgc
     3001 tttaaattac tatagtatta gcgattatgc gtggggtagt agaaaatggc attagagggc
     3061 ccgaagtaga ctattccatt gaataattta atatagcatc agttttttgc atgtagtttg
     3121 gattctgctt gaaaagcgat gccatttttc actaccccat gaagcaaagt attctcttca
     3181 gcaaaacacg caaagaatct ccaaaagacg aggtttcaaa aaatgccgat cttttgatac
     3241 gtgcagggta tattcataaa gaaatggccg gtgtctacac ttttttaccc ctcggtttgc
     3301 gggtgctttc gaacattaac gaaattgtca ggcaagaaat gaacaaaatt ggcgggcaag
     3361 aaatgttcct ttctgccctt caggagagaa cgatttggga aaagactggc cgctggagcg
     3421 atcagtctgt agacatttgg tttaaaacga aactcaaaaa tgacacagta ctcgggctcg
     3481 gattcacgca cgaggaacca ttgaccaacc tgatgcgcga tcatattcgc tcatggcgcg
     3541 atctgcctcg atacgtctat cagattcaga cgaaattcag aaatgaaatt cgcgcaaaaa
     3601 gtggcatcat acggggcaga gaatttttaa tgaaagatct ttattctttt tgccgcgatc
     3661 aaaaagagca cgatgccttt tacgaaatag ttaaggatgc gtatatgaaa attttcgcgc
     3721 gcgctggaat tgcggacaaa acatacatca cgtttgcttc aggaggaagt ttttcaaaat
     3781 attcgcacga atttcaaaca ctcacgtccg ccggtgaaga cacaattcac ctctgtctaa
     3841 actgcaagat tgcgatcaat gacgaaatta tcaaggaaaa tcctgcatgt ccgagatgcg
     3901 gaaatagaaa gttaaagcgg gaaaaatcca ttgaagtggg aaatattttt agcctaggaa
     3961 cgcgattctc ggaggctttt gggctggtct atgtcgacga gaaaggtgca aaaattccgg
     4021 taattatggg aagttatggt atcggccccg gacggctcat gggagtgatc gcggaactgc
     4081 tttcggatga gaagggcctt atctggcccg aagaagtagc cccatatgaa attcatttga
     4141 ttgcaataaa tgacaaaaca ggcgctgtcg tcgggctcgg agagaaatat gaggcagaat
     4201 tggaggagaa gaatatttcc gtgctctttg acgaccgaga tgcgcgggca ggtgagaaat
     4261 ttgcagatgc ggatttaatc ggcattggaa gacaagtgat tgtaagcgaa aaaaatagtg
     4321 cggctggaat tgttgagatc aaaagtcgta tgaccggcac cgcagaaaaa aagaaatggg
     4381 agacattcgt gcaggaaatt acacatgaaa aatgacacaa tcgaaaaaat caaaagatat
     4441 ctatcgaacg acatcggcat agatctagga actgccaata cgctcgtgta tcttcgtggt
     4501 aggggaattg tgatcaacga accttccgtt gtggctgtga accaaaaaac ggggcgcgtg
     4561 gttgcagtgg gcatgcaggc gaaacagatg cttggacgga cgccagcgca tattcgcgcc
     4621 gtgaagcccc tcgttgacgg agtgatttcg gactttgagg tgacagaaga gatggtcgcc
     4681 tatcttctca ataaggccga gaaaaattcg agaaaatttt taggtccgcg ggtcgtcgtc
     4741 ggtgtgccgt atggcatcac caatgttgaa acgcgcgccg tacgggatgc cacgaaaaac
     4801 agcggtgcgc gcgatgtgta tattgttgaa gagccgatgg ccgcggcgct cgggatcaga
     4861 ctgccggtca aagaacctgt cggtagtatg attatagata ttggaggcgg aacctgcgat
     4921 attgccgtta tttctttggg tggaattgtc cgcgccaaaa cgctgcgtat cgccggagat
     4981 aaattgaacg atgatatcat ttcttacgtc cgaagcgaat ttaagatatt gatcggtgag
     5041 aaaactgcag agacgattaa aatgacgatc ggatccgtga tgccgctcgg atcgccgctt
     5101 gagtgtccgg tgaagggaag agatttggta actggattgc cccgggaagt tattctcacc
     5161 gattcggata tacgcgaggc gatcgctctc tccatcgata cgattatcga atctgtgaaa
     5221 gaagtgcttg agacgactcc gcctgaaata gtttcggatg taatgcatcg cggcgtccac
     5281 gttgtgggcg gtggcgcgct tctgcgcggt attggtgctc tcttgaatga ggcagtgcgc
     5341 atacccattt atattgccga cgacccgctt actgccgttg cgcgaggcgc aggaattatt
     5401 cttgaagata tagaaagtta tcgggatatt ttgatacaaa acgaagatga ccttccacat
     5461 caaaaataga caaaagcgtt tttcctatct cgtcgcaatt attgcgctac tcatcgtcct
     5521 tctcgcttgg attcgcccga gttttttggg tggttttaca catcaaatcg gccgaccgat
     5581 ttgggcaagt gcgcgtggcg tacgagcgct cacaagaact ataactatag gctttagcgc
     5641 aaaagagaag ctggcggcag agaatctacg gctcaagcga gaacttcaag gtatcgagac
     5701 tataattatc cgttcggaat cgctcgagcg ggaaaacgag gcactgcgcg cgcttaccgg
     5761 aaagaaaccg gcgaacacat ttctttttgc ccgaacgctg accaaagttg gattttcacc
     5821 gtatgacaca tttgtactca acgtgggtga gcgcgacgga cttatgaaaa aagggggact
     5881 atacacacta gaaggattgc tggtcggcta tgtcgatcag gtttatgatc acacgtcagt
     5941 ggctcgactc tattcgtcgc ctggcgagga atttcgagtg ctcgtcggaa cggaagaatt
     6001 acccggcacg gccataggac gaggcggcgg aaactttgag cttaaactac cgcgggatct
     6061 taaagtaaaa aagggcgatc acatcatggc agaagacgat aaacatggcg tggcaggcgt
     6121 ggtagaagac atagaactcg acgttacaaa ttcgcttcaa actattctct tcaggcaacc
     6181 cgtaaatatt tacacgatta cagaggtttt ggtggaaaaa aatatcaatt gatatgaaat
     6241 tgcgattttt ttgtaccatt ttgcttctcg ttctcatttt tttgtcacca tggtggctta
     6301 cctttgcggt cgcacttgca ttgattttct attttcgtag atattatgag gctttttttg
     6361 cggctttcct gttcgattta ctctataaca cgtctcgagc ccacctgttt ttctttgcgg
     6421 gcttggctac tgttgcggta agttttgtct taattgagtg gctcaaaaaa cgtcttagcc
     6481 tctacgaagc gatctaatgt gatgtggttt tataaaagag aaaaaaaaag cacgcgagaa
     6541 atcgaacccg atgaaatttt tcttgattct cacaatttgc ccgaatttga tgtgcatcaa
     6601 tttgagggcc gtctcgagaa accgattgcc atacgaacgg tttttattgt ggccgtcttt
     6661 tttatagcta tagggcttgc atatgcttca agactttgga tgttgcaaat tgcgcatggc
     6721 gaggcgtacg cggaacgcag tgaaaaaaat cgcctgcgtc ataagatatt tttttcagag
     6781 cggggcgttg tctatgatag gaatgatatt ttactcgctc gtaatgttcc gaacgatgac
     6841 acgccggatt ttgcgtcacg cgtatacagc gacgaaccgg ggcttgctca tgtgctcggt
     6901 tatgtaaaat atccagcact agataagtct ggcttttact acagtctcga gtacagcggt
     6961 gtggcgggtg tagagaaact ttacgacgac gaacttcgcg gaaaaaatgg cttgaaaatc
     7021 attgaaacag atgcgctcgg cgagataatt tccgaaaata tcattgagcc gccggagccg
     7081 ggcaaaaatg tcacactttc aattgatagc gaagttcaaa gtgaacttgc gcgaatcata
     7141 aaaaatttaa gcaacgaggt cggttttgaa ggcggcgcgg gtgcactcat ggacctcaaa
     7201 accggagaac tattagcact caccagcatc cccgaattta gctctgatgt catgtcggaa
     7261 ggggaggacg aggtaaaaat acaagaatac ctggaaagtt ctcaaaaact ttttctcaat
     7321 cgtataaccg gaggcctcta tacgcccggg tctatcgtca aactgtttat cgcactcggc
     7381 gcactaaagg aaaaaatcat cacccctgaa catgagatac tttcaacagg atcgatcagt
     7441 attccaaatc cctatgattc caaaaaagaa tctattttta aagattggag ggctcacggg
     7501 cttgtggaca tgcgccgagc gattgctgtg tcatcgaacg tgtacttttt tgaagttggt
     7561 ggcggctaca aggatcaaaa gggtctcggg atagaaaaga taggcgaata catgaatctc
     7621 ttcggtttcg gaagacctg
//
LOCUS       DATDSU010000008         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011 k141_6323,
            whole genome shotgun sequence.
ACCESSION   DATDSU010000008 DATDSU010000000
VERSION     DATDSU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6323"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VI981_00195"
     CDS             <1..923
                     /locus_tag="VI981_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4478773.1"
                     /translation="RRFLKYGPVLIVIAAVLWGLDGILRRSLFTLPPIIIVFYEHLIG
                     LIIIAPFLIKRWKQEKLTKNEWWAVGFVSLLSGVLGTLWFTTALAKVHFIPFSVVFLI
                     QKLQPIFAIAASAILLRERITRKYAVWAVVALVSAYFVTFKNGIVNFGEGGAVIIAAL
                     FALGAAFAWGTSTAFSGFTLVRHSHTFITGLRFLITVPLAFILLLFMGKASMLNDLTS
                     SNITRLIIIALTTGMVALWIYYRGLALTQAKIATILELVFPLTAVIIDIFLYKTFLAP
                     TQYIAALVLLFAVYKVSRLNSQEANVPLAG"
     gene            949..1266
                     /locus_tag="VI981_00200"
     CDS             949..1266
                     /locus_tag="VI981_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEY4478774.1"
                     /translation="MNFPTLNLEIPEQFVYEEGIYAGWVYLDGEKHPAAFHYGPIPAF
                     NDFAVSLEVFVIDKVISVQPTVVDFEMQKRIRDILNFDTKDLLAHQMALDVKQTKIIL
                     GVQ"
     gene            complement(1268..2242)
                     /locus_tag="VI981_00205"
     CDS             complement(1268..2242)
                     /locus_tag="VI981_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY4478775.1"
                     /translation="MIKKALITGAAGFLGSHLCDRFIREGFFVYGVDNFITGDRANIS
                     HLEKHPQFQFIEQDVCKPLSIKDGLNYILHFASPASPIDYLKIPIETLKVSSQGAYAI
                     LDLAKDKKTRVLMASTSEVYGDPLVHPQTEEYYGHVNPIGPRSCYDEAKRFMEALTMA
                     YHTVYGMETRIVRIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTMFGDGSQTRSFCYV
                     DDLIEGIYRLLMSDYYLPVNVGNPDEITIKEFGAEILKLTGSKQKLISLPLPKDDPKQ
                     RRPDISKARKILGWEPKVSRADGLKITYEYFKKMPKEALYKTEHRKFS"
     gene            complement(2245..3144)
                     /locus_tag="VI981_00210"
     CDS             complement(2245..3144)
                     /locus_tag="VI981_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY4478776.1"
                     /translation="MSLHFHLRTWFVGTFVIVTLTAIVYLAVKQRSSREELKAEQARN
                     ASSTAAFIKRVGDLEIGLADLTNQSLQLVELLKAEQSQSGAFQNEIKKIADTVGTLDK
                     LRKTDPRLLQKYSKVYFLNEHYAPTDLVSIPIGYASSADKLDLKIHSGVWPHLDQMLT
                     YASSSGNHIKIVSAYRSFGTQATLKSNYTVIYGSGANKFSADQGYSEHQLGTTLDFST
                     AVLGNNFSKIDMTNEYKWLENNAHFFGFTLSYPKGNGYYEYEPWHWRYVGVELASRLY
                     KDGKHFYDLEQAEIDKYLISIFD"
     gene            3264..3506
                     /locus_tag="VI981_00215"
     CDS             3264..3506
                     /locus_tag="VI981_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478777.1"
                     /translation="MLATTNETALLAEADALATSDFEQSLRAVQGDYESVRAAVAPLS
                     RLVERMQRSTIVILIGWCRSRSLEATADWLEAQVCL"
     gene            complement(3567..>3930)
                     /locus_tag="VI981_00220"
     CDS             complement(3567..>3930)
                     /locus_tag="VI981_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478778.1"
                     /translation="DYCIENVGACVSSYRLPWSCPEDEQSDMFETAVLHAVGHVMGLA
                     LPSSEDESEYLAHCRSSSCVMANAENNAQRLRAMTEVRMKSINPFCGTCTKYLREGDY
                     FLQERPAPSASPSPSQVF"
BASE COUNT         1022 a          894 c          898 g         1116 t
ORIGIN      
        1 tgagaagatt tttaaaatac ggaccggtac tgattgtgat cgccgctgtt ctttggggcc
       61 ttgacggtat tttgcgtcgc agtcttttca cgttaccgcc cataataatt gttttttacg
      121 aacatttaat cggactcata ataattgcgc cgtttttgat caagcgatgg aaacaggaga
      181 aactgacaaa aaatgaatgg tgggccgtcg gatttgtaag tctcttgagc ggtgtattgg
      241 gaaccttgtg gtttacaact gcgcttgcaa aggtacactt cattccgttt tcagttgtgt
      301 ttctaattca aaagcttcag ccgatatttg ccattgccgc ttcggcaatt ttattaaggg
      361 agagaattac acgtaaatat gccgtatggg cggtcgtggc gcttgtttct gcatactttg
      421 tcacgttcaa aaacggaata gtaaattttg gggaaggcgg tgcagtcatc attgccgctt
      481 tgtttgcgct gggcgcagca tttgcctggg gaacgtcaac cgcattttcg ggttttactt
      541 tagtccgcca ctctcacaca ttcattaccg gcctgcgttt cttaattaca gttccactcg
      601 cttttatact tctcttattt atgggaaaag cttcaatgtt gaatgatcta acttcgagta
      661 acatcacacg cctcatcatt atcgcgctaa caacgggaat ggtcgccctc tggatttact
      721 atcgaggcct tgcgctgact caagctaaaa ttgcgacgat tctcgaattg gtatttccgc
      781 taactgccgt tataattgac atttttctgt ataagacctt tcttgcgcca acccaatata
      841 tcgccgcgtt agttttactt ttcgccgtct ataaagtatc acggctaaat tcacaagaag
      901 ccaatgtacc gctcgcgggt taaaagtggg aagggcaggg gaaaggagat gaattttccg
      961 acactgaacc ttgagatacc cgagcagttt gtgtacgaag agggtattta cgccggctgg
     1021 gtatatttag acggcgaaaa acaccctgcg gcgtttcatt atggacctat cccggcattt
     1081 aacgactttg cagtttcgct cgaagtgttt gttatcgaca aagtaattag cgttcagccc
     1141 accgtagtcg atttcgagat gcagaaacgc attcgcgata ttttaaattt tgatacgaaa
     1201 gatttgcttg cacaccaaat ggcgttagat gtgaagcaga ctaagattat tctcggagtg
     1261 cagtgatcta ggaaaacttt cgatgctccg ttttgtagag agcctctttg ggcatttttt
     1321 tgaaatattc gtacgtgatc ttcaaaccat cggcacggct cactttcggt tcccagccta
     1381 aaattttacg agcttttgaa atatccgggc gtctctgttt aggatcgtct tttggcaggg
     1441 gaagggaaat gagcttttgc ttgctgccag tcagtttcaa tatttccgca ccgaattctt
     1501 tgatggtaat ctcgtcggga tttccgacgt tcaccggtaa gtaatagtca ctcatgagca
     1561 gacgataaat tccctcaata aggtcatcaa cgtagcagaa acttcgtgtt tgtgaaccgt
     1621 ctccgaacat tgtgagatct tcgccacgca gagcttgccc aataaaggca ggaaggacgc
     1681 gtccgtcgtt caggcgcatg cgcgggccgt acgtgttaaa aatccgtaca atccttgtct
     1741 ccattccgta tacggtgtga tatgccatcg taagcgcttc cataaaacgt tttgcttcat
     1801 cgtaacaact gcgcgggcca ataggattta cgtgtccata atactcttcg gtctgtggat
     1861 gcacgagcgg atccccgtac acttcacttg tagaggccat gagtacccgc gtctttttat
     1921 ccttggcgag atccaaaatc gcatacgctc cttgggacga cactttcagt gtttcgatcg
     1981 gaatttttaa ataatcgatc ggactcgctg gagacgcgaa atgaagaatg tagttgaggc
     2041 cgtcttttat tgaaagaggc ttacatacat cctgctcaat gaattgaaat tgcggatgct
     2101 tctccaaatg tgaaatatta gctctgtcac ccgtaatgaa attgtcaact ccgtatacaa
     2161 aaaaaccttc acggataaac cgatcacata aatgcgaacc caagaatcca gccgcacccg
     2221 ttatcagagc ctttttaatc atggctagtc aaaaatacta attaaatatt tatctatttc
     2281 cgcttgctca agatcataaa aatgcttccc gtccttgtaa agacgcgacg ccagttcgac
     2341 gcctacatat ctccaatgcc acggctcata ttcatagtaa ccgtttcctt ttgggtacga
     2401 gagagtaaaa ccaaaaaaat gggcgttatt ttcaagccac ttatattcat tggtcatgtc
     2461 tattttactg aaattatttc cgaggactgc cgttgaaaag tcgagtgtgg tgccgagttg
     2521 atgctcggaa tatccctgat ccgccgaaaa tttattggct ccagatccgt atattacagt
     2581 gtaatttgac tttaatgttg cctgcgttcc aaatgagcga tatgcagaga caattttaat
     2641 atggttcccg gaactcgaag cataggtaag catttgatca aggtgcggcc aaacacctga
     2701 atggattttg agatcaagct tgtcggcgct tgaagcgtat ccaatgggaa tcgacacgag
     2761 atcggtggga gcgtaatgtt cgtttagaaa ataaaccttg gagtatttct gcaagagacg
     2821 cggatcggtt ttacggagtt tgtcgagcgt cccgactgta tctgcaattt tttttatttc
     2881 gttttgaaat gcaccgcttt gcgattgctc cgccttcaaa agttcgacaa gttgaagact
     2941 ctgattggtg aggtcagcga gaccgatttc aaggtcacca actcgcttta tgaaggctgc
     3001 cgtactcgat gcgttccgtg cctgctcggc cttgagttcc tcacgagacg atctctgctt
     3061 cacggccaaa tatactatgg ccgttaaagt aacaataaca aaggtaccga caaaccacgt
     3121 tctcaaatga aaatgcaggg acataaaaaa cattctacca tcttcgtgtt gtactaaaaa
     3181 catgcgtttt cagactctcc gtgatgtggt actttatcga gcgggtagtc ataccggtat
     3241 ccaacattta aatatgcgtg attatgctcg caacaaccaa cgaaacagct cttctagcag
     3301 aagcggatgc gctcgcgacg tcagacttcg aacagagcct gcgtgcggta caaggtgact
     3361 atgagtcagt gagagccgct gtagctcctc tttcacggct cgtcgaacga atgcagagaa
     3421 gcaccatagt aatcttgatc ggctggtgcc gatcaagatc gctagaggca accgctgatt
     3481 ggctcgaagc ccaggtttgc ctttaactaa cactgtctcg ccgatagtgt tttttttaca
     3541 taaaaaaaca gggctgaagg cctgttttaa aaaacttggg aaggcgatgg ggaagcggaa
     3601 ggagcgggcc tctcctgcag aaagtagtcg ccctcgcgta aatacttcgt gcaggtgccg
     3661 cagaacggat taatgctctt cattctgacc tcggtcatcg cacgcaatcg ttgcgcatta
     3721 ttctcggcgt tcgccataac gcacgaactg cttctacagt gggcaagata ctcactttca
     3781 tcttcagaag atgggagcgc aagacccatg acatgtccga cagcatgaag tacggcagtt
     3841 tcaaacatgt ccgactgctc gtcttcggga cagctccacg gcagacgata gctcgacacg
     3901 caagctccga cattttcaat gcagtaatcg
//
LOCUS       DATDSU010000009         6987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2180601, whole genome shotgun sequence.
ACCESSION   DATDSU010000009 DATDSU010000000
VERSION     DATDSU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180601"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /gene="mraW"
                     /locus_tag="VI981_00225"
     CDS             <1..391
                     /gene="mraW"
                     /locus_tag="VI981_00225"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEY4478779.1"
                     /translation="VEARAKTAIKSTADLVRIIKEATPAAYQRGKIHFATRTFQALRI
                     VVNDEIASLKEGLQNSWKVLAPSGRIAVISFHSIEDRIVKRFGRGKKACGEAVILTKK
                     PITPGDDEISRNRRSRSAKLRIFEKIK"
     gene            430..765
                     /locus_tag="VI981_00230"
     CDS             430..765
                     /locus_tag="VI981_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478780.1"
                     /translation="MNRVLANTYTIERQQKLFWLIAIAVGLTLLFYGYLVNRTVFNVA
                     SWGRDNSELSKTRSTIGELELAYFDSKNNISPEMARKIGFSEVVTPIFISRTQIPSVI
                     SLNTREKIQ"
     gene            783..2483
                     /locus_tag="VI981_00235"
     CDS             783..2483
                     /locus_tag="VI981_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY4478781.1"
                     /translation="MSSVFRTRIWFLGACFIVLTLVLSAKLYTVQIVNGAAYKERAER
                     QYERPAETAFNRGSIYFTNKDGSLVSAATLKTGFILALMPKYIEDAEKTYEVLSQILE
                     IDKDDFLKKAVKKDDPYEEIARRVPSETADEIRRAKFPGVELYSEKWRFYPANKVASD
                     VLGIVAFKDKTLAGRYGLEKYYDDVLVRDESDLYSNFFAEIFSNVRELGKGKPLEGDL
                     VTTIEPNVSLFLERELGKVVDTWGAKQAGGIVMDPYTGEIVSLIEHPSFDPNNFKEET
                     TRVFGNALVEDVYEMGSIIKPLTIAAGLDSGAITAKTKYNDEGQMTIDNATISNFDGK
                     ARGTVNMQEVLNQSLNTGAVFAVNRMGKDKFRSYMKSFGLGEETGVDLPNETAGIIDN
                     LDSPRDLEYATASFGQGIAMSPLITIRALASLANGGYLPNPHVVSKIDYKVGISRTLS
                     YDEKRRVIKSETSEEISRMLVEVVDKALLNGTVKMTNFSIAAKTGTAQIPKVGGKGYY
                     DDRSLHSFFGYFPAYNPRFIIFLYAVEPKARYASETLTKPFINLVKFLISYYEVAPDR
                     "
     gene            2537..3844
                     /gene="murF"
                     /locus_tag="VI981_00240"
     CDS             2537..3844
                     /gene="murF"
                     /locus_tag="VI981_00240"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY4478782.1"
                     /translation="MKQFFKKIIVTIITLEARVVLWKYKPKIITVTGNVGKTTTKDAI
                     YAAFSTTHHVRRSEKSFNSEIGIPLTILGCENAWHNPFRWIENILKGLFVIVIQIRYP
                     EWLILEIGADRPGNIKNITSWVRPDVSVITRFAKVPVHVEYFSSREELIAEKAYAVKR
                     CKPRGVVVLNADDADSLAMKSITSCHVVTYSLNGAADIVGSHEQIYYEKDDRGIEVPA
                     GMTFKVDFQGKSVPVVRKAVLGLQHVYPTLAAMAVAVSQKINIVSASEALSHDTTPPG
                     RMKLLGGNKRTSIIDDTYNSSPVAVHEALETLRLIKTKGRKIAVLGDMLELGKHSIEE
                     HKHIGKLVAGITDILVTVGIRARYIAEGALNFEMDEAKIFQFDESREAGRFLDPLIKE
                     GDIILVKGSQSIRMERVVEEIMAEPLKKEVLLVRQDAEWQKKA"
     gene            5092..6084
                     /locus_tag="VI981_00245"
     CDS             5092..6084
                     /locus_tag="VI981_00245"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HEY4478783.1"
                     /translation="MRKILISLGMIVFVGAVVAGATGAFFSDTETSTGNTFAAGAIDL
                     GIDNESYYNGALNQGTSWSLNFDLDKGCRNPAFPGTNPDTQQPEPEFIPCLFFNFDDV
                     KPGDYGEDTISIHVNDNESWLCADVSLDSDDDVTCTEPENDSDAENGACQDSDTNPTD
                     GVGDNGNNADGDLADSLNFLWWADDGDNVLEDHEQTLPAGPIGALSVGATTTVTLADS
                     LYNIWTGQSGPFPAGDTRYIGKAWCFGPIQASPLSQSSLPQGRPTTTPAQDGSGNSVS
                     GEPADGGYLCDGESVGNEAQTDKAKISIAFRAIQSRNNGLFQCKPELFPLDVDY"
     gene            6285..>6987
                     /locus_tag="VI981_00250"
     CDS             6285..>6987
                     /locus_tag="VI981_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HEY4478784.1"
                     /translation="MRRIILGLLLIVGAGAVILTGATGAFFSDTETSTGNTFAAGAID
                     LQVDNESYYNGNKCAENPLAGEAHPNEPYFIWQGNAPYPVPGTPCTISWELDDLDNGR
                     LFFNFTDLKPDDESEDTISLHVGTNDAYACMDVTLTSNDDNSSTEPELDTPDQPEDIN
                     NTWDGELAQNLEFFWWADDGDNVYEEGENSISDGVQALYDLATTSPFSVALADSQNNV
                     WGDTGPIPGDKIVYIA"
BASE COUNT         2123 a         1418 c         1693 g         1753 t
ORIGIN      
        1 cgtagaggcg cgtgcaaaaa cagccataaa gagtacagcc gatcttgttc ggataatcaa
       61 agaagcaacg ccagcggcgt atcagagagg gaaaatccat tttgcgacga gaacatttca
      121 ggcacttcgg attgtggtca acgacgagat cgcgagtctc aaggaaggac ttcaaaattc
      181 ttggaaagtg ctcgcgccga gtggccgaat cgccgttatt tcttttcaca gcattgaaga
      241 cagaatcgtt aaacgatttg gcaggggaaa aaaggcttgc ggggaagcag tgatacttac
      301 aaaaaaaccg ataacacctg gggatgacga aatatcacgc aatagacgat cacgttcagc
      361 aaaacttcgc atcttcgaaa aaatcaaata actacaacag aggaaattgc caagcgggca
      421 aaaacaaaaa tgaacagagt acttgcaaat acatatacaa tcgaaaggca gcaaaagctg
      481 ttttggctta tcgcgatagc cgtcggtctt acgctgctct tttacggata tctcgtaaac
      541 cgtaccgttt tcaacgtagc ttcatggggg agagacaata gtgagctctc aaaaacaaga
      601 agtacaatcg gagaacttga actcgcctat tttgattcaa aaaacaatat tagccccgaa
      661 atggctagaa aaatcggctt tagcgaggtc gttacgccga tatttatcag cagaacacaa
      721 attccttcag tgatttcgtt aaatacccga gaaaagatac aataacctct taaaatatag
      781 agatgagttc ggtatttcgc actagaatat ggtttttagg agcatgcttc attgtgctca
      841 cgctcgtgct aagcgcaaaa ctctatacgg ttcagatcgt gaatggtgcg gcctacaaag
      901 aacgggcaga gcgccaatac gaacgtccgg cggaaactgc gttcaaccgc ggttcaattt
      961 attttacgaa caaggatggg tcgctcgtgt cggccgcgac attaaaaacg ggatttatcc
     1021 tcgcacttat gccgaaatac attgaagacg cagaaaaaac atatgaagta ctttcacaaa
     1081 tactcgaaat cgataaggac gattttttaa aaaaagcagt gaagaaagat gacccctacg
     1141 aggaaatagc gaggcgcgtg cccagtgaaa cggccgacga aatccgccgt gccaagtttc
     1201 cgggtgtcga actctatagc gaaaaatggc gtttttatcc ggctaataaa gttgcatcgg
     1261 acgtgctcgg gattgttgcc tttaaagaca aaacacttgc gggacgctac gggcttgaga
     1321 aatattacga cgacgtactc gtgcgtgacg agtcagatct ctatagtaat ttttttgcag
     1381 aaatattttc gaacgtgaga gaactcggaa aaggcaaacc gcttgaggga gatttagtca
     1441 cgacgattga accgaatgtt tcgctttttc ttgaacgaga gcttggaaaa gttgttgaca
     1501 catggggcgc aaagcaggca ggagggattg tcatggatcc atatacggga gagattgttt
     1561 cactgatcga gcatccatca ttcgacccga acaattttaa agaggaaact actcgcgtat
     1621 tcggaaatgc gcttgttgaa gatgtttacg aaatgggttc aataataaaa ccgctgacca
     1681 ttgcggctgg gctcgatagc ggtgccataa ccgccaagac aaaatacaat gacgagggac
     1741 aaatgaccat agataacgca actatttcta actttgacgg taaagcgcgt gggacagtga
     1801 atatgcagga agtgctcaat caatccttga atacgggcgc ggtttttgcg gtaaacagaa
     1861 tgggaaaaga caaattccgt tcatatatga agtcgtttgg cctcggtgaa gaaaccggtg
     1921 ttgaccttcc gaatgaaacg gcaggtatta tagataactt ggacagccct cgggacttgg
     1981 agtatgcaac cgcctcgttc gggcagggaa tagctatgtc acctctcatt actattcgag
     2041 cgcttgcctc tctcgctaac gggggctatc taccaaaccc gcacgttgtc agcaaaatcg
     2101 actacaaagt gggcatttcc cgaacgctaa gttatgacga gaagcgacgc gtcatcaaaa
     2161 gcgaaacatc agaagaaata tctcgaatgc tcgtcgaagt cgtcgataaa gcgcttttaa
     2221 atggaactgt taaaatgaca aatttcagta ttgccgcaaa gacaggaaca gcccaaattc
     2281 caaaagtcgg cggtaaaggg tattatgatg accgctctct ccactcattt ttcggatatt
     2341 ttcccgcata taatccaaga tttatcattt ttctctatgc cgtcgagccg aaagcccggt
     2401 atgcctcgga gacgctgacc aagccgttta taaatttagt taaatttttg attagttatt
     2461 atgaagttgc gccggatcga tagggaagcg tttagggtaa agcgtccagc gtttagtgaa
     2521 caaaaagaga actgacatga aacaattttt taaaaaaata atagttacga taatcacgct
     2581 tgaggctcgc gtggtgcttt ggaaatacaa gccaaagata attacggtta ccggaaatgt
     2641 gggtaaaacg acgacaaaag atgccattta cgccgccttt tcgacgacgc atcatgtccg
     2701 tcggagtgaa aaaagcttta acagcgagat cggaatcccg ctcactattc ttggctgcga
     2761 aaatgcgtgg cacaatccgt ttcgttggat cgagaatata ttaaaggggc tctttgtgat
     2821 tgtaatacaa atacgctacc cggaatggtt aattcttgaa atcggagccg acagaccggg
     2881 aaatataaag aatataacat cgtgggttcg tccagatgtc tctgttataa cgcgcttcgc
     2941 taaagttcct gttcatgttg agtatttcag ctctcgagaa gagctaattg ccgaaaaagc
     3001 ctacgcggtt aagcgttgca agccgcgagg cgtggttgtc ctaaatgccg atgatgctga
     3061 cagtctcgcc atgaaatcaa ttacgtcatg ccatgtggtt acgtacagtc tgaacggagc
     3121 ggcagatatt gtcgggtcac acgaacaaat ctattatgaa aaagacgatc gagggataga
     3181 agttccggca ggtatgactt ttaaggtgga ttttcaaggt aaatcagttc cggttgtgcg
     3241 aaaagcggtt ctcggacttc agcacgtata tccgacactt gcggccatgg cggtggcagt
     3301 ttcccaaaaa atcaatatag tttcggcttc ggaagcgctt tcccacgata caacgcctcc
     3361 tggaaggatg aagcttttag gcggcaacaa aaggacttcg ataattgatg atacttataa
     3421 ttcctcaccg gtcgctgtcc atgaggccct cgaaacctta agactaatta aaactaaggg
     3481 acggaaaatc gcggtcctgg gggacatgct cgaactcggg aaacactcga ttgaggaaca
     3541 caaacatatc ggtaaattgg tcgcgggcat tacggacatt ttagttaccg taggcattcg
     3601 tgcgagatat attgcggagg gcgccttgaa tttcgaaatg gatgaggcaa aaatatttca
     3661 atttgatgaa tcacgcgaag ccggacgttt tcttgatccg cttataaagg aaggcgatat
     3721 tattctcgtc aaaggctcac aatcgatccg aatggagcgg gtcgtcgaag aaatcatggc
     3781 tgaacctctg aaaaaagaag ttttgctggt gcggcaggat gcggaatggc agaaaaaagc
     3841 gtagacaaag cagtcgcgct gatccgaatt ccatgttcgg acgcatctag ttcctgaatt
     3901 ttacgtatag attataacta cattctatac ccggcgtttt tagttcccga tatctttcgg
     3961 tccatgactt ttatagatat tttatgtgca tattttaatc aactaattcc tcttattaac
     4021 taagtcttgt agctctactg cggggtagta gtgctgggta gttggtgagg ggtagttgat
     4081 tgcgggctaa ttgatcgtgt atcttataac tatactaacc tcatgcttat agcacgaagt
     4141 tagtatatgg ggttatttgc gaattttagg tttagatata agtttaggtt ttgggaatga
     4201 aaaagacaac aaatattgtt tgatgattgt gtggtattag aaaagattaa tggacaaaat
     4261 taatgggagt atcgaaaaga gcgggaaatt gaccccgtta gaagcagaac gccccgtggc
     4321 ttgccgcggg gatgactgca gtttggattt agcaaacaca gggtttaatg ccccgtgtga
     4381 gcttctaacg gggttgacga cgaggggact aaaatgaagc agacgatact tgataagtct
     4441 tgcttgtagg agagtcagat gttgataaag gcacacgtct cccttaatag cataatgcga
     4501 taaatgtgca gctcgtcaat atacattaaa attacgcggc cgcgtaattt taatgtatat
     4561 tacaaagaca tgctcgcgaa gttgctttta ggggcaacat tccgcgattg ctattgtgtg
     4621 gtgaggggag tagcgtggat aaaggtgtgc gtaaacctgt ggatatggtg ttaataggct
     4681 gttgataaag tgtcgaaaat ttgtgcataa atatactgtc ctaaagacaa aattcaaaag
     4741 tcaaaaatgg ttcaaagacc catcgcgtaa aagtcataaa tgaagccgtt attttgtcca
     4801 taaagaacac aaaaattgac agtcaaaaaa ataatattgt tgagtggggg aaaaggacac
     4861 gcaaaagttg tactcgggca aatttaggaa gagaatgaaa ctacaacctt agcgaaccaa
     4921 caatccgtta agtaattgca tggtttccct gcatcttaaa aaatcagatt actgactcgg
     4981 agtctttcgg gttttttaag ctatggaaat catgcgttgc gaagcaaacg caacaaacaa
     5041 atggaggtcg gtccattcat taaaaaataa ataaaaataa ataacttaac aatgcgaaaa
     5101 atacttataa gtttgggaat gattgttttc gttggagcag tagttgccgg agcaactggc
     5161 gccttctttt ctgacacaga aacttcaaca ggaaacacgt ttgcggctgg ggcgattgat
     5221 ctcggtatcg acaacgagtc gtactacaac ggtgcgctga atcagggtac gtcctggtcg
     5281 cttaatttcg acttagacaa gggatgtcga aatcctgcat ttccaggaac aaatccagac
     5341 acccaacaac cagaaccaga gtttatccca tgtctattct tcaatttcga tgatgtaaag
     5401 cccggagact atggtgagga cactatctca attcatgtca atgacaatga atcgtggttg
     5461 tgtgccgatg tctcacttga ttctgatgat gatgtgactt gtactgaacc ggaaaatgat
     5521 tcagatgcag aaaacggcgc atgccaagac agcgacacga acccgactga tggcgtagga
     5581 gacaatggca acaatgctga cggtgatctt gctgatagtc tcaacttcct ctggtgggca
     5641 gacgatggag ataacgtgct ggaagatcat gaacaaacgc tccccgcagg acctattgga
     5701 gcactttcgg tcggtgcaac gaccacggtt acacttgcag actctctcta caatatctgg
     5761 actggacaaa gcggtccatt cccagcggga gacacgcgct acataggaaa ggcgtggtgt
     5821 ttcggtccaa ttcaggcttc gcctttgtca caaagctcat taccgcaagg ccgaccaacg
     5881 acaacaccag cacaagatgg tagtggcaac agcgtaagcg gagaaccagc agatggagga
     5941 tatctctgtg acggtgagag tgttggaaat gaagcacaaa ctgacaaggc aaaaattagc
     6001 attgcgttcc gcgcgattca gtcacgaaac aacggactat tccagtgcaa acctgaattg
     6061 ttccctcttg atgtggacta ctaatcctct ctagccgttc cgagcaccag aaatggtgct
     6121 tggttggctg taggggatcc cgttagagct agcggaaatc aaaagatttc ctacctctaa
     6181 cgggacgagc tcccaattag agatggcgcc cgcctcaggc gaactacctc taacgggaca
     6241 agttcccata gcactaacta tcaattatta attattagaa atacatgcga cgaattatct
     6301 taggacttct ccttattgtg ggcgcgggtg cggtgatatt gacgggagcg actggtgcgt
     6361 ttttttcgga taccgaaacg tcaacgggaa acacgtttgc ggctggagcg attgatcttc
     6421 aggttgataa cgagagttat tacaacggca ataaatgtgc ggagaatcca cttgccggag
     6481 aggcacaccc aaatgagcca tattttattt ggcaaggtaa tgcgccctat ccagtgcccg
     6541 gaacaccatg tactataagt tgggaactcg atgacctcga caacgggcga ctcttcttta
     6601 atttcaccga cttgaaacct gatgatgaaa gtgaggacac tatctcgctt cacgttggaa
     6661 caaatgatgc gtacgcatgt atggatgtaa cgctgacttc aaatgatgac aattcctcta
     6721 cagagcccga actggatact ccagatcagc cagaagatat aaacaatacg tgggacggag
     6781 aacttgcaca gaatctagag tttttctggt gggcagacga tggagataat gtttatgagg
     6841 aaggagaaaa ttcaatttcg gatggggtac aagcgctcta tgatctagcg acgacaagtc
     6901 ctttttctgt tgcacttgcc gattctcaaa ataatgtatg gggtgacact ggaccaattc
     6961 caggagataa aatagtttat attgcta
//
LOCUS       DATDSU010000010         4143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_342881, whole genome shotgun sequence.
ACCESSION   DATDSU010000010 DATDSU010000000
VERSION     DATDSU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342881"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2053
                     /locus_tag="VI981_00255"
     CDS             <1..2053
                     /locus_tag="VI981_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478785.1"
                     /translation="IAHAQFFEPTLIETLHFTIEDVSASCVADCFSNVLFLPGLESSR
                     LYRPNVIGPEIRVWEPSFFGLDHEQLLMNPDGTPVKTWIYTKDVIDEAFTANIYKSFI
                     ERMDELVADGTINEWNDFPYDWRFDINDVVDNPTALATSTISLIAEVERLAAGSKSGK
                     VTIISHSNGGLVGKVLLSELESQGKADLVDKFIMVAVPQLGTPKTIATLLHGEAMPSQ
                     LFPFVAKAQEIREMAENMPSGYTLLPSTEYFSRVLDPVVEFVDNGSPLMQPLVDSYGL
                     AITNTTELNDFLLGADGRSKPAESDVMNPNIMNSDLITQGAANHIVLDGWDVPDGIEV
                     IQIVGWGVPTIRGIRYKEGRLTGAACSISNCSFIDHEPILTIDGDETVVVPSTSLLTI
                     DTYYVNLLEYNRFKNLKINREHADIMEVDQLLSFMTNLIRNDTTVPSFIETSKPTFDP
                     ESAPALRISIHSPVSLDLYDTPGRHTGIATSTLSGNSFIEEQIPNSYYIEMGEGKYAG
                     ADILSTTTVRLHGLALGTFTLDIEQTLGDTITASTTFGNIPVTTSSTALLDIGDSTNL
                     PVSKLSVDVDGDGVTDADISGGDGLTVPELLGLLRGLVQSLNLPLKKENKFIKAIDKL
                     QKQILKNQNGKKFQKYRIIDALKDIKDKIKTFEKKKLLAKDEVKELLDILSRVQNLVL
                     N"
     gene            2058..2501
                     /locus_tag="VI981_00260"
     CDS             2058..2501
                     /locus_tag="VI981_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478786.1"
                     /translation="MDYKRRLIFLGTLALAGLLIASVLLWPDRLGLCSHGDKACIYNY
                     INYEIISVPILLFSLLTLVCSVVLYFMRSGVFYSWVKFTLWWVPLSVILIAITPEYGG
                     SGLDGGIPSFGREVVIWFMAGGFFVLSLLIIAWKSFRLRKKEKSI"
     gene            2548..2826
                     /locus_tag="VI981_00265"
     CDS             2548..2826
                     /locus_tag="VI981_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478787.1"
                     /translation="MTAEKIIQLIFILAGFCLIFLIVSAFFITAETGSTLETLLLISI
                     IPVIMAGVIFVILARTRYNTVLPPFLRVIGLILFALWIASFFVDSPFR"
     gene            3365..3433
                     /locus_tag="VI981_00270"
     tRNA            3365..3433
                     /locus_tag="VI981_00270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3395..3397,aa:Thr,seq:tgt)
     gene            complement(3862..>4143)
                     /locus_tag="VI981_00275"
     CDS             complement(3862..>4143)
                     /locus_tag="VI981_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478788.1"
                     /translation="GFNFAKETLADSSGGIENVPQVLGATSSKSNAAQTNKGAVGENK
                     PSFFSKLAQLASPAILFSFGSRAIFYPLLIILALGLLYILGRIVNRPKY"
BASE COUNT         1171 a          872 c          958 g         1142 t
ORIGIN      
        1 cattgcgcat gcgcaattct ttgaaccgac tctcatcgaa actctgcact tcacgatcga
       61 agacgtatcc gcttcgtgtg tcgcagattg tttctcgaac gtgctctttt tgcccggcct
      121 tgaatcgagc aggctatatc ggccgaatgt catcggcccc gaaatacggg tatgggagcc
      181 gtcatttttc ggtctcgacc atgagcagtt gttgatgaat cctgacggga cacctgttaa
      241 aacatggatt tatacgaagg atgtaattga tgaagcattt actgcgaaca tatataaatc
      301 tttcatcgag aggatggacg aacttgttgc agatggaact atcaatgagt ggaatgactt
      361 tccgtatgat tggcgattcg acataaatga tgtagtggat aatccaacgg ctctcgccac
      421 gagcaccatt tcactgatcg cggaagtgga acggcttgct gctggatcga aatcagggaa
      481 agttactata atttctcatt caaacggcgg tttggtaggc aaggtgctct tgagcgaact
      541 cgagtctcaa gggaaggcag atctcgtgga taaattcatc atggttgcag tgccgcagct
      601 gggaacgcca aaaacaattg ccacattgct gcacggggaa gcgatgccct cacaattgtt
      661 cccatttgtc gcgaaggcgc aagaaattcg cgagatggcg gagaacatgc cctcgggcta
      721 tacattgctt ccgtccactg aatacttctc gcgcgtgctc gatcccgtcg ttgaatttgt
      781 tgataacggc tcaccactga tgcagccatt agttgatagc tatggtcttg ctattaccaa
      841 tacgactgaa ttgaatgatt ttttattggg cgctgacggt cgttctaaac ccgcggagag
      901 cgacgtaatg aatccaaaca ttatgaactc cgatctcata actcaaggcg cggcgaatca
      961 catagtgctc gacggctggg atgtgcccga cggaatagag gtcatccaaa ttgtcggctg
     1021 gggagttccc acaattcgcg gtattcgcta caaagaaggt cgactcacag gagcagcctg
     1081 tagcatctcg aattgcagtt ttatcgatca tgaaccaatt ctcacgattg atggagacga
     1141 gaccgtagtg gttcccagta catctttatt aacgatagat acctactatg taaatttgct
     1201 cgaatataat cgatttaaaa atctgaagat caatagagaa catgccgata tcatggaagt
     1261 ggatcaatta ttgtcattca tgacaaatct aatacggaat gacacaacag ttccatcatt
     1321 tatagagact tccaaaccaa catttgatcc cgaaagtgcc cccgccctga gaataagcat
     1381 tcattcacct gtctcgcttg atctttacga tacgcccggc cgtcataccg gcattgcaac
     1441 ttccacgctg tcaggcaatt cttttatcga ggaacaaatt ccaaacagct actacatcga
     1501 aatgggcgaa ggcaaatatg ctggggcaga catacttagc actaccactg tccggctaca
     1561 cggccttgcg ctcggcacat tcactctcga tattgaacaa acgttgggag atacaattac
     1621 tgcatcaacg acattcggaa acattccagt aacaacaagc tcgacagcct tgctcgacat
     1681 tggagactca actaatctac ctgtttctaa attgtcggtg gatgtagatg gcgatggtgt
     1741 gactgatgct gatatctccg gtggtgacgg actcacagtg ccggagttac tcggtctctt
     1801 gcgcggcttg gtacagtcac ttaatttgcc cttgaagaaa gagaataaat tcataaaagc
     1861 tatagacaaa ttgcagaaac aaatcttgaa gaatcagaac ggcaagaagt ttcagaaata
     1921 ccgcataatt gatgctctca aagatataaa agataagatt aaaacgtttg agaagaaaaa
     1981 attacttgca aaagatgagg tgaaggaatt attggatatt ttatcacggg ttcaaaattt
     2041 ggtgctaaac taactccatg gattataaga gaagacttat atttctgggc acattagccc
     2101 ttgcgggtct tcttatagcc tcagttcttt tgtggccaga tcggttggga ttgtgcagtc
     2161 atggcgataa ggcctgcatc tacaactaca taaactatga aattatttcc gtaccgattt
     2221 tattgttttc actattgaca ctagtctgct cagtcgttct atactttatg cgatcaggcg
     2281 tgttttattc gtgggtgaag ttcacgttgt ggtgggtgcc tctctccgta attcttattg
     2341 caattacgcc agaatatggg ggcagtggtc ttgacggggg tataccgagc tttgggcgag
     2401 aagtagttat ctggtttatg gccggaggat tctttgtact ctcacttctc atcatagcct
     2461 ggaagtcatt tcggttgcgg aaaaaagaga aaagtatata ggtcggttct cggggtacca
     2521 caaagacacg aaaattcaaa acaaaaaatg actgcagaga aaatcataca gctcatcttc
     2581 attctcgcag gattctgttt gattttctta atagtatctg cttttttcat tacagcagaa
     2641 acaggaagta cgctcgagac cttgctgttg atatcgataa ttcctgtaat aatggcagga
     2701 gtgatttttg tcatactcgc gcgaacaaga tacaacactg tacttccacc attcttgcga
     2761 gttatcggtc ttatcctttt tgccttgtgg attgcatcgt tttttgtcga cagcccattt
     2821 cgctagggat gactttgcat cgcactgtta tgaaaaatct ctataacatc acaagaggac
     2881 aacttattac gctgtgggtg tttggaatct ccgcgattat ttgggcaatg gacgcatcat
     2941 gcaattagta tgcagtgtgc atgcctcaac tgagaagaga tacgactgtt gaggatatcc
     3001 tcatatttct aatccccttt gcactgattt tctacacgat cggctggttc aatcatcgaa
     3061 aacaccgcga gtaagttccc atacatactt gcctttattc actagttgtt gcgcaataat
     3121 ctagggaaat agtcgcctga atacggggac acgaagttgt ccacaaagca aattaaaaaa
     3181 ttatcaagtc gcatatgtta gtattatttt agcttaaata aaggaaataa tgcgtctttt
     3241 tggatattag agcaagtttt acgtgcggaa acccagtcaa tagctgacaa agcgcaggac
     3301 tttgtcattt tttgactggg ctttttgttt atcaaaacaa aaagcacccc tgtggagttg
     3361 gtaacgggac gtctttggaa gaagaagcgg ttcttgtaaa accgtaacgc gagttcgaac
     3421 ctcgccgtcc cgatactgcc tctaaaggtg ctctttgcct aacccacacc acccgcaaag
     3481 cgatgagtgt tgagattaat gatactacct caatacgaaa gtctagcaag ctggtgtgaa
     3541 tagcgagtga atctaaggca gcaaaaggtc gttattcgga gaattaaaac gagaaaaaat
     3601 atgttgaaga atgaaaaaac ttctcgcggt gttggtacgt tggcatctaa aattttgcgc
     3661 ggcgataaga ggccgacaat aaaagatgca aaacgtttag ccgcgagtgt tctcacccag
     3721 cggccagata gaaggcgcta gggaaatagg taatgttttg tataggttgc gggattacac
     3781 aaaacgtgcc gatagtagag ccccaacgtt gagttggggc tctcgtcgtt ggtgccatat
     3841 gatcccaaat tcgagaacaa actaatattt tgggcggttg acgattctgc cgagtatata
     3901 tagaagtcct agagcgagaa ttattagtag gggatagaaa attgcgcggc tgccgaaact
     3961 gaaaagaatt gcaggcgacg ctagctgtgc gagttttgag aaaaatgaag gtttgttttc
     4021 gcccacagcg cctttgttag tttgcgcggc attcgattta gaacttgtgg cgcctaaaac
     4081 ttgcggcaca ttttctatac cgccggaaga atcggcaagc gtctccttcg caaagttaaa
     4141 tcc
//
LOCUS       DATDSU010000011         4483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_841407, whole genome shotgun sequence.
ACCESSION   DATDSU010000011 DATDSU010000000
VERSION     DATDSU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841407"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VI981_00280"
     CDS             complement(<1..975)
                     /locus_tag="VI981_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY4478789.1"
                     /translation="MNLRKSKTSKVMAGLIGFSMILSLFVGVGVQTASAALTQAQVDS
                     IISLLQSFGADASTINNVRTSLTGGTPTGGGGTPSTGGFVFNNNLSLGMTHSDVLQLQ
                     LVLNADSATQVSTSGAGSPGNETNYFGTRTRAAVINFQAKYGISPQSGFVGPLTRQEL
                     NSMGGVVVLPPPPPPGTPLPPPVAGTGVTISAAAQPANSLAVINAARVPFTRFTLTAG
                     SDGDVTVNSVTVERQGLATDGNFAGVVLLDEMGTQVGLAKTLNSNHQVILNEPLKIMR
                     GSSKTYTVAVNMQDTNAEVNGGEIAAFAVINVNSAATVTGTLPIVGASH"
     gene            complement(1854..2642)
                     /locus_tag="VI981_00285"
     CDS             complement(1854..2642)
                     /locus_tag="VI981_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478790.1"
                     /translation="MDSEPKKDTNFPKKTYQSLGEFYYGDSPALIHVFNKTEKITLAV
                     YLISDVFFEQEPLKWKLRNLGLNLISGLSSVSGSFSSHTLFNLYVELSALFRLALRSR
                     LVSEMNYNVLNKEIEGVMSLLVQYNSQQNINEEIFNRNDGIVLDEISQEFTKKDTLDT
                     VGKGQSKGQYRTSFIAPHGRKTEAKSTPSEDNGSSTSERREIILGVLKNKEWISINDV
                     SLLISGCSEKTIQRELLSMVEAGILVKEGERRWSKYKLAQNGHI"
     gene            2833..>4483
                     /locus_tag="VI981_00290"
     CDS             2833..>4483
                     /locus_tag="VI981_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY4478791.1"
                     /translation="MSKRNKDQLNLFSRLNVFRNTKQETLQTILAIIFLALALFFILA
                     PFEKSGVVGARVYIFLKYLFGIGYLLVPLFLIILSIAFFKNLKKEFHISKIGGALLSF
                     LSGLGLVDIAVDSEAGVVGHYISSPLLRFFDIYISIIFLIALLVATLLFLFDAHFSLQ
                     QLLLWKKWKKKDINKEVDVKITSDAADVSKIKNKDNEDASGQTEGDSLAEKIILAKTQ
                     TSGKAKKEGEESDLPFPAPQPYHSQTYIPIPISLLEGDRGKPGVGDIKANANIIKRTL
                     QNFGVNVEMDEVSIGPSVTRYALKPAEGVKLSRIVGLQNDLALALAAHPIRIEAPIPG
                     KSLVGIEIPNTTKTTVGLASLLSSSEFRESQKPLLLSLGRGVSGKSHYANLAKMPHLL
                     IAGATGAGKSVTIHSIITSLLYRNSPENLKFMMIDPKRVELTLYNKIPHLLTPVITDP
                     KKAILSLKWAAKEMDRRYDVLETEAVRDIESYHKNILEPELRKLGGKSTTEQQNMKVP
                     ELMPYIVIVIDELADIMATYPRELEAAIVRLAQMSRAVGIHL"
BASE COUNT         1271 a          986 c          895 g         1331 t
ORIGIN      
        1 atgagatgca ccaacaattg gaagtgtacc cgtaacagtt gctgctgaat taacgtttat
       61 tacagcaaag gcagcgattt caccaccatt tacctctgcg ttagtatcct gcatgttaac
      121 agctactgtg taggtctttg aacttcctcg cataatcttg agaggttcgt tcaaaataac
      181 ctgatgattt gaattgagtg tctttgcaag accaacttgc gtgcccatct cgtcgagaag
      241 gacaacccct gcgaagtttc cgtctgttgc aagtccttgt cgctctacag taacgctgtt
      301 tacggttaca tcaccgtcgc ttccggctgt aagagtaaat cgtgtgaatg gaactcgcgc
      361 cgcgtttatt acagcaagag aatttgctgg ctgtgccgct gccgagatcg ttactcccgt
      421 tccagctact gggggaggaa ggggtgttcc cggaggggga ggaggaggta gaactaccac
      481 tccacccatt gagttcagtt cttgtcgtgt gagtggacca acaaatcccg actgtgggct
      541 aattccatat ttcgcttgga aatttatcac tgcggcccga gttcgggtac cgaagtaatt
      601 tgtttcattt ccaggagaac ctgctcccga tgttgatact tgagttgcac tgtcagcatt
      661 taggacgagc tgaagttgca atacatcgga gtgtgtcatt cccaagctaa ggttattgtt
      721 gaaaacaaat cctcctgtgc ttggagttcc accaccaccg gttggcgtac cccctgtaag
      781 agaagttctg acgttattga tcgttgaagc gtcagcaccg aaagattgaa gcaatgaaat
      841 aattgaatca acctgtgcct gtgtcagagc cgcgctagca gtctgaactc ctactcctac
      901 aaagagtgag aggatcatgg aaaatccgat gagtcccgcc ataacctttg aagttttaga
      961 ttttcgtaaa ttcataaatg tttataatca atcgtctaat ttttttcgct aattactaat
     1021 aaaagcactt cgtcttgtat ttcgacttcg actcggacga tgtgtctccg cttttcggaa
     1081 acactcgctc ctcgctcgtc gaaataagaa ttatgctacg aaaaaaatta ttttacagaa
     1141 aacagtagtt tcgacttaaa acaactgccg tccgtgtgtg caagttcgta acttacacag
     1201 tgagtcttgt aagaagagac ttcctacaac actcctttac tattccatct aattaattat
     1261 tagctctgat tttcctttgc aggtagtcag agaaatcgac aaaaaatcaa atggattagt
     1321 gaacattgta attctgtaaa tcagccgtaa tcagtacatg ccattactca tggcaaatcg
     1381 ggacaaagta ttactgtcat tttattctct ctattctgtt gtcaatgagc acgaacttct
     1441 ctctctaaac aattctcaac tttaatgaga gagctgaata tttttagctc aaaatagagc
     1501 caaaaatatt cacaaggtaa ttatatgcaa gcagtcacat ataactagtt cactcggtcg
     1561 atgcgttcca ccttaggcgg accgctcgaa ccttgttgcc aattatatga ttggtgttac
     1621 atataattgg ttcactcgga cgatgtgcaa gacttcgtct ttcactcgat cctcgttgcc
     1681 aattataatc ttagttgtga aagctcgcaa aaatcctttg tggataactc catatgcaaa
     1741 caaaatccgc gttacgcgcg taatttatga aaaacatatg taatttaccc attgacatca
     1801 tgttcgcaca gtaacaatat ttagtcaact tgagttatag gccccaaaaa tgtctaaata
     1861 tggccatttt gagccagttt atacttactc caccgtcgtt caccttcctt taccagaatg
     1921 ccggcctcca ccatcgaaag aagctctctc tgtatggttt tctcactgca tcctgaaata
     1981 aggagagaga cgtcattgat agatatccat tctttatttt taaggactcc taatattatt
     2041 tctcgtcttt cacttgtgct cgatccgtta tcttcgctag gtgttgattt cgcttcggtt
     2101 ttcctgccat gtggggcaat aaaggacgtc ctatattgtc ctttagactg tcctttaccg
     2161 acagtgtcta acgtgtcttt tttggtaaat tcttgggata tctcgtccaa gacaattcca
     2221 tcattgcgat tgaaaatttc ttcattaatg ttttgttggc tgttatactg taccaacaaa
     2281 gacattactc cttcaatctc cttgttaaga acattgtagt tcatttcaga cacaagccta
     2341 gatcggagag ccaaacgaaa caacgcggag agttccacat atagattaaa taacgtgtga
     2401 ctggaaaaag acccggaaac tgatgacagc ccagaaatta ggttaagtcc tagatttctc
     2461 aatttccatt taagtggttc ttgttcaaag aaaacatcac ttataagata aacggcaaga
     2521 gttatttttt cagttttatt gaagacgtga atgagcgctg gtgaatcccc gtaatagaat
     2581 tcacccaagc tttgatatgt ctttttgggg aaattggtgt cttttttggg ctcactgtcc
     2641 atgagatatc ttcttaaaca ctcgcgtggt cattttgata ttatgtccat tatattaaag
     2701 acagctctag atgcaatggt ttaaatttct agagctgaaa aaaaagctcg tgtaaagctc
     2761 gcttaggcga gtcatgatat tattaaccat gtttagattc atattaaatg caatgattga
     2821 tatgtacgag atatgtcgaa gagaaacaaa gaccaattaa accttttttc aagactgaac
     2881 gtttttagaa atactaaaca ggaaacactt caaacgattc ttgcgattat atttctcgcc
     2941 ctagccctct tctttatact cgcccctttc gaaaaatcgg gtgttgtggg agcaagagtg
     3001 tacatattcc tgaagtatct ttttggtatt ggatatttgt tggtgccgct ttttttgatc
     3061 attctttcaa ttgctttttt caagaaccta aaaaaggagt ttcacatttc gaaaataggt
     3121 ggagcgttat tatcatttct ttctgggctc ggtcttgttg atattgcagt cgacagcgag
     3181 gccggggttg taggacatta catttcttct ccccttctac gcttctttga tatatatata
     3241 agtattattt ttcttatagc ccttcttgtt gcaacgcttt tgtttctttt tgacgcacat
     3301 ttttccctcc aacagttatt gctatggaaa aaatggaaaa agaaagacat taacaaagaa
     3361 gttgatgtta agatcacttc tgacgctgct gatgtttcaa aaataaaaaa caaggacaat
     3421 gaggacgcga gcggacagac cgagggagac tcgctagctg aaaagataat ccttgctaaa
     3481 acgcaaacat cgggcaaggc gaaaaaagag ggggaagagt ctgacttacc cttccccgcc
     3541 ccccaaccct atcattctca aacgtacata ccgattccga tttctctact tgaaggtgac
     3601 agaggtaaac ccggggttgg cgacatcaaa gcaaacgcga atattatcaa acgaacactc
     3661 caaaatttcg gagtaaatgt tgaaatggat gaggtttcaa tcggtccctc ggtgacacgg
     3721 tatgcactca agcccgccga aggagtcaag ctttcccgaa tagtcgggtt acaaaacgat
     3781 cttgcgctcg ctcttgcagc tcacccaatt cgtatcgaag caccgattcc cggaaagtct
     3841 ctggtcggca tagaaatacc aaacacgaca aaaaccactg tcggactggc ttcgcttctt
     3901 tcctcttctg aattcagaga atcacaaaaa ccgctccttt tgtcgctcgg ccgaggagta
     3961 tcaggaaaat cacattacgc gaatttagcc aaaatgcccc acctccttat tgcaggggcc
     4021 accggtgctg gaaaatcagt aactatacac tcaatcatta cttccctttt gtatcgaaac
     4081 tcaccggaaa atcttaaatt catgatgatc gatccgaaac gtgttgaact caccttgtat
     4141 aacaaaattc cacacctact tacccctgtt atcactgacc ctaaaaaagc gattctctcg
     4201 ctaaaatggg cagcaaaaga aatggatcga cgctacgacg tactcgagac agaagccgtt
     4261 cgggacattg agtcgtatca caaaaacatt cttgagcccg agcttcggaa gcttggtgga
     4321 aaatctacca cggaacagca aaacatgaag gtgccggagc tcatgccgta tattgttatt
     4381 gttattgatg agctcgccga tatcatggcg acctacccgc gtgagcttga agctgccatt
     4441 gttcgcctcg cccaaatgtc ccgagcggta ggtattcatt tga
//
LOCUS       DATDSU010000012         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2185191, whole genome shotgun sequence.
ACCESSION   DATDSU010000012 DATDSU010000000
VERSION     DATDSU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185191"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VI981_00295"
     CDS             complement(<1..1349)
                     /locus_tag="VI981_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478792.1"
                     /translation="MKITMHHFKKILGLVIFALIFAFPPSVVATVYTGPTGIIFSDFD
                     EVGLPSTGFKDILVDCSVNNGRDVNINSLKDGSILGGGHGCDDRYDNFWMQDNMVVNG
                     VSTDEQIADGHYCIFINGVGAPVCTHGEFDILDGLLVPQNPEPCTENCFSNVLFLPGI
                     KGSVLKTESDTLWPPTALSDDVGELALTVGGESVNEIFTDGILNEFYGTEIYGPFSQF
                     MDGLVADETIQEWQPLAYDWRFSPERVVEDGIKTSNGTIDVMERIETLAESSRTGKVT
                     IVAHSMGGLLGKAVIKKLEDEGKENLIDSFVMVGTPQLGTPQAVGAILHGDGESIAAG
                     FITSAVTIRSVAQNMPSAYVLLPSPKYFDEVEDPLIVFDEEASFTDGWRSMWGDFINA
                     YSGFASFATGGGGARDNPDESVLRMPEVLQSALVDDANQFHNEYDNYQFPEHIRVVQV
                     "
     gene            complement(1551..1659)
                     /gene="rrf"
                     /locus_tag="VI981_00300"
     rRNA            complement(1551..1659)
                     /gene="rrf"
                     /locus_tag="VI981_00300"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1754..1826)
                     /locus_tag="VI981_00305"
     tRNA            complement(1754..1826)
                     /locus_tag="VI981_00305"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1791..1793),aa:Trp,seq:cca)
     gene            complement(1851..1924)
                     /locus_tag="VI981_00310"
     tRNA            complement(1851..1924)
                     /locus_tag="VI981_00310"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(2061..>2252)
                     /locus_tag="VI981_00315"
     rRNA            complement(2061..>2252)
                     /locus_tag="VI981_00315"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          664 a          559 c          433 g          596 t
ORIGIN      
        1 acttgcacca cgcgaatgtg ttcgggaaat tggtaattgt catattcatt atgaaattga
       61 ttagcatcat caactaatgc agattgcaaa acttccggca ttctcaagac actctcatct
      121 ggattgtcgc gcgccccgcc accccccgta gcgaatgatg caaaaccaga ataagcattt
      181 ataaaatctc cccacataga gcgccagcca tccgtaaagg aagcctcttc gtcaaaaact
      241 attagcggat cttccacttc gtcgaaatat ttaggagaag gcagtaaaac atacgcactc
      301 ggcatatttt gcgctaccga gcgtatggtg acagcactcg tgataaagcc ggcagcgatg
      361 ctttcgccat cgccatgcaa aattgctccg acggcttgag gcgttccgag ctgtggagtc
      421 cccaccataa caaaagaatc aattaagttt tccttcccct catcttcaag tttttttatt
      481 acagccttgc ctaaaagtcc tcccatagag tgagcaacga tagttacttt ccctgtccga
      541 gaactttcgg caagcgtttc tattctttcc atcacatcta tagttccatt gcttgttttt
      601 atgccgtctt ctactaccct ctccggagaa aatctccagt cgtaagcaag cggttgccat
      661 tcctgtatcg tttcatccgc aaccaagccg tccataaatt gggagaacgg tccatatatt
      721 tccgtgccat aaaattcatt caaaattcca tcggtaaata tttcattgac gctctctcct
      781 ccgaccgtaa gcgcgagctc tcctacgtca tcgcttaatg ccgtcggagg ccaaagcgtg
      841 tcggactctg tcttcaaaac gctcccctta atgcctggca agaaaagaac attggaaaaa
      901 caattttccg tacagggctc ggggttctga ggtaccaaca aaccatctaa tatatcaaac
      961 tctccatgcg tgcacactgg agcacctacg ccatttatga aaatacagta atgcccatcc
     1021 gcaatctgct catctgtact tacaccattc acaaccatat tatcctgcat ccaaaaattg
     1081 tcatatctat catcacagcc gtgaccaccg cctaaaatag aaccatcttt tagagaatta
     1141 atattcacat cacgaccatt gttaacagaa caatcaacta gaatatcttt aaaaccggtg
     1201 gagggcaggc caacctcgtc aaaatcagag aaaataatgc ccgtcgggcc tgtgtaaact
     1261 gtagcgacaa cagagggagg gaatgcaaaa atcagcgcaa aaatcacgag tcccagtatc
     1321 tttttaaaat gatgcatagt gattttcata ctaccaatat ttcataaaac gtcaatacct
     1381 atgtgaaata gttgaaatta tgtaaaataa aacaaaaaaa ttgcaatcac accactttta
     1441 gacggccgtc tgaaagtggt gtggatttgt aatcaagatg ttttgaggaa ttagaaggaa
     1501 agaaaaaaaa ccagtaattg tttattggtt tttttgcgtt taaatacatt gttctggcgc
     1561 ctagctactt ttgccaaaaa gactatcatc gccactagcg ggcttaattg ctctgttcgg
     1621 aatgggaaga gatgtatctc cgccgtcaaa gcaccagaac attgtattca aagcaaatca
     1681 aaatctgtag agtgggcacg aatttctccg tacccactcc tggggaggaa aaattcgtgc
     1741 tgatgatact gcacttggga ggagggattc gaaccctcgg ccggcggttt tggagaccgc
     1801 tgctctgcca actgagctac tcccaaacaa tacaaggtac aaatacggca agcgccccac
     1861 cggactcgaa ccgatgacct cctctcgtgg aagcaggtgc tctaaccaac tgagctagag
     1921 gcgcaaagaa cgaaagtgaa ataacttacc cactttacag attttgattt ctacccgcac
     1981 acttatttgc ttgatgttta aaacatgcgc ttatagcgcc tcaagcacat aagtgtgcgg
     2041 gttaattgtg gtttccaaat ttcttaacag gaatcgaaac attagtactt ctcagctgaa
     2101 gcgattactc gccttacact taaagcctat caacctagtc atctcctagg gttcttaacg
     2161 attcctaatc ttgaagctgg cttccctctt agatgctttc agaggttatc cattcccgac
     2221 atagctaccg agcagtgccc ttggcaggac ag
//
LOCUS       DATDSU010000013        15980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_174132, whole genome shotgun sequence.
ACCESSION   DATDSU010000013 DATDSU010000000
VERSION     DATDSU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15980
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174132"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..499
                     /locus_tag="VI981_00320"
     CDS             236..499
                     /locus_tag="VI981_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478793.1"
                     /translation="MAEKKQSRKKDAYTIVLEHIHSDFKVFGEGLSLIRDEVKGNSRR
                     LDKVEERLANLEVEVALIRHNQVTRDEFKFLETRVARLERKLK"
     gene            590..907
                     /locus_tag="VI981_00325"
     CDS             590..907
                     /locus_tag="VI981_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478794.1"
                     /translation="MDQNTQIFTSKKSYFKGGLYLGIIIGIVTGVFSFLRLDNYAQVI
                     TLVPFLAFLLYILGFNNGKGITDKNWFYFVWGYIIPLAIVFLISFPIAMWCKSNASAS
                     ICF"
     gene            1381..1617
                     /locus_tag="VI981_00330"
     CDS             1381..1617
                     /locus_tag="VI981_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478795.1"
                     /translation="MRGKSYDALKLNQTETPLLIFMESYNKSVPASFPHASVKVLKQF
                     QILHPILFKNSDEWSIDKHRKKLMDWLPSHLDIA"
     gene            complement(1642..2712)
                     /locus_tag="VI981_00335"
     CDS             complement(1642..2712)
                     /locus_tag="VI981_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4478796.1"
                     /translation="MPQKISSPSFFIGKGDDSLISFGSGQPDLPPPKGAYSILKHYKA
                     FKYGLIQGEENLRSSLAREYPNAKPEHFVITNGASEALDLVLRVLSRPGGRILLPRPY
                     YYSYPHNVLYAGMRPAYYDLDKNGKIDFEIFKKAVQGCRAVIINSPSNPTGTVQEIAT
                     LKKIERLCGTLGVYVISDEVYKDLIYVRKNYLIQGEKVVTVNSFSKTYAMCGFRVGYL
                     YSKNIEVIEKVIEMKTHTSMNTSIVGQAMALEAMKSPRSYIAHHVKIWEERRDMIYGG
                     MHDLGLELWKPEGAFYVLPKLKNPNRVVNDLYYKYKMIAYDGTWFGAPDRVRFSYALD
                     ISKIKEGLRRLKTFFGGEYKKY"
     gene            2910..3761
                     /locus_tag="VI981_00340"
     CDS             2910..3761
                     /locus_tag="VI981_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4478797.1"
                     /translation="MNFLFPIVAALLQASSATLDKVLLSFKRVDYKVYTGVSYPLLFV
                     ITLVIFLVVRPPLSRELFRDDLFFLLLISVAFTTITNLIVYRALKHERLIEIEAVGLL
                     QTLPIIVFSSIVFTDERRYAVVVPAVIAAFAIIWSHWEHHHFQMNKDTYPLLIWLLVA
                     APVGAVLSKELLRVWDPISLELVRSFLVAILLYKYFERGFAKITLPIFGLFVLTNLFS
                     SVAWILVFYGYQRIGIIHTMLLFSLQPMLVYFASAFFLKERFSWKKSAAFIVVLLSIA
                     TAQLLGV"
     gene            3873..4124
                     /locus_tag="VI981_00345"
     CDS             3873..4124
                     /locus_tag="VI981_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4478798.1"
                     /translation="MDSKPIGTVTHYYDKAGVAVVKFASAVSVGDKIKIVKGDHEFEQ
                     EISSMQIEHEQITSAKKGSEVAIKVGEPTKEGAVIYKVE"
     gene            4183..4255
                     /locus_tag="VI981_00350"
     tRNA            4183..4255
                     /locus_tag="VI981_00350"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4216..4218,aa:His,seq:gtg)
     gene            4377..5795
                     /locus_tag="VI981_00355"
     CDS             4377..5795
                     /locus_tag="VI981_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4478799.1"
                     /translation="MLRYTLRVRDLVHGTILFTEQEENIINHPLFQRLRQIRQNDVAF
                     FVYPSLNTSRLEHVLGTCRVAGMMAEHLTQSPKWRTYLKELKTETWIVSKEQFIELCR
                     LYALLHDIGHLPLSHLFEMAVESWAGKPKGVIAEEWTGIGGFTKLHEAFGASIVKRMV
                     PSIKLSKPVGSALLKVMTEKDLPFKHPLSVVKKLVDFDIDADRTDATQRDGFSAGGEY
                     GHYDIRRLCDAVFIEQDDQEWIVSYSEKALTSMEALLLDRYRIHLWVHFHHRVVAMKM
                     LVGFLIEIALRRKLITKDHFDPKKADAFVLKDDVWLWGVLREMPTGGDRTLEMVKRAV
                     FFREKRNTFNLWKTRPGYHALRTRAADGAGVATFTITDLDAYCRYLSKRMKIRVLGFK
                     PPFEPISDKKVLLYSEVEKKLTGKEIREVSRLVAGLKTLWENDPQEFHLLVGKDARVR
                     RARLRDRWVEETADWIRSPRLL"
     gene            5841..6059
                     /locus_tag="VI981_00360"
     CDS             5841..6059
                     /locus_tag="VI981_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478800.1"
                     /translation="MKKFYYPDHDKRYAHIPKSQRRLYPDEIHIGSAVIKRTKLYKDI
                     TGKDIRKYGVDACLIAKDVPYTEGLYDG"
     gene            6349..7014
                     /locus_tag="VI981_00365"
     CDS             6349..7014
                     /locus_tag="VI981_00365"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HEY4478801.1"
                     /translation="MEVSSINSKFIFKVLSIGAMLFISYAASLFVQSVVNDRIAYQTE
                     ALKTQSGDTSRVLMHGKLPGFDEEGISTYRLVDRVLKYAILFISLTFIAFFLTEVTHQ
                     LKLHPIQYLLIGLGLAEFYLLLLAFMEHIGFLWAYIIAAAMTILLISLYSYFILKNKK
                     GGALIAILLTFIYSYLLAALNLETYALLSGALLLFVVLSGVMFITRNLNWYDAFSYVK
                     KAE"
     gene            7165..7374
                     /locus_tag="VI981_00370"
     CDS             7165..7374
                     /locus_tag="VI981_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEY4478802.1"
                     /translation="MTTLSVPVPSHLEEFINKQVRLGKASNKAAVIRRALQLLAEEEA
                     VEAVLKAETEPTLRGNLRALAKKLK"
     gene            7371..7634
                     /locus_tag="VI981_00375"
     CDS             7371..7634
                     /locus_tag="VI981_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478803.1"
                     /translation="MIHVSFKPTFIRQFNVLEGDLQGEVVEKIELFKDRKNHKQLKIH
                     KLKGRLAGRYSFSVNYQIRIVFIYGQKDEAILLAIGSHDVYRS"
     gene            8169..8381
                     /locus_tag="VI981_00380"
     CDS             8169..8381
                     /locus_tag="VI981_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478804.1"
                     /translation="MKITYLGPAGATFSAVAYNELSRLFRVPSTNDEGIELVLAKTND
                     EIDCPSLEPPEARPLKDCFGSFRIPN"
     gene            complement(8551..9651)
                     /locus_tag="VI981_00385"
     CDS             complement(8551..9651)
                     /locus_tag="VI981_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4478805.1"
                     /translation="MRKLRIATMVTGHFATPPPADVIYAPMDIAVAVSEGLAKRGHSV
                     TFFAPEGSRIKVSKIETVGLKPLRHNGELAILAGPNVGGAERAKIYGLWDQFLLSAIY
                     KEALKSKFDIIHVHPVDRALPLAYAIRNVPTVYTLHDPIYKWRAEVFRMFSSPNQYYV
                     SISDSQRLPAPDLNYLATIYNGINTKEIPFYEKPKGNYLLFVGRLHPEKGVAEAVEVA
                     QKTGDDLIILGPPVTGEYWDKKVKPYLGEKIRHIGVVSRKELYEYYGNAKATLVPIQW
                     EEPFGLVMIESMATGTPVIAFCRGSVPEIVADGKTGFIVNTMEEMVEATKKVNAINRN
                     NCRVHIEESFGIKRMIDNYEKAFMRLVENKSV"
     gene            complement(9648..10817)
                     /locus_tag="VI981_00390"
     CDS             complement(9648..10817)
                     /locus_tag="VI981_00390"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY4478806.1"
                     /translation="MRTKSVTTKKEIAARLEADMGKLEDREHGYIRAGLPRFPALFGR
                     DSCIVSWQLIDYDPNIARRTIKILARLQGKKFDNASEEEPGKIVHEWHPNPAEYKALA
                     WALPYYGSVDATPLFIYLCGLYYEKAVNTEWLAAYWPHIISALEWCEKYGDFDGDTLL
                     EYERKNSTGLMHQGWKDSRLNHLNINSPVELIEVQGYYYAALREASELALMLKDTKLA
                     RCLADRSEKVKENCIKKFWLADENFFASALAESNVADRRVMSNPGHILFTGILDGYDD
                     KIKMTVDRLFQDDMRTPYGIRTHAESNFDFNPMSYHLGSIWPHDNWIIAQGLKKYGYE
                     KEYQKIKRGLLDAYSELGEIPELYAVTDGKIERIPVACSPQAWASGALLNFVLEK"
     gene            complement(10799..11911)
                     /locus_tag="VI981_00395"
     CDS             complement(10799..11911)
                     /locus_tag="VI981_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4478807.1"
                     /translation="MKKSMKSLYKKFGKRKLRIAQVAPLWFTIPPKGYGGIERIVAML
                     TEELMRRGHEVTLFASPGSTTSARLISVFDKSLSEANIPWSNPIWNLRNLSRAFEMAE
                     DGKFDIVHCHLDLWGLFFQGIVQTPIIHTMHNPLYRTNADATKDDRLRLFGEEAHRTD
                     IVFISESARKNSMVEFSKGRVIYNGIDLEHFRFNKKGGEHFIWIARMNKHKGAENAIE
                     AAEKFGAKLLLAGRIDPTQEEYFEKIIKPHLNQNIRYVGELGDNKLSDFYGRAKALLY
                     PIEWEEPFGLVVAEAMACGTPVIAYRRGSMKELIKDGETGFVVDSSIELLTDAMKKIN
                     TIDRAAARKHIEEKFSKERMADAYEKTYYELCERKA"
     gene            complement(11883..12926)
                     /locus_tag="VI981_00400"
     CDS             complement(11883..12926)
                     /locus_tag="VI981_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4478808.1"
                     /translation="MHKKKPRIAQLAPLWIPIPPRTYGGIELMLSELTEELKKQGYDI
                     TLFASGDSKTSAKFVPVIEKAIWLDRELRNPHAPVMRMLKEVFERFYEFDVLHNHFNF
                     FMFPLSLRLDCPRFLTTVHQPVDKQYAETMKAYHKIKYVAISEDHKRSMEAFGVPVCD
                     VIYNGIDPARYEFNDSSGDYLLYLSRLNREKGVLTAIEVAKAAGQKLIIAGNSAGNAE
                     WSFFLHEIQPHLHDDNISFRGQVNFQEKVELLKNAKALLFPIDRQEPFGLVMIEAMAC
                     GTPVIGFRKGSVPEVIEHGKTGFVVSTNEEMINVIERISAIDRRACRKAVEEKFSLEK
                     MVDKYEKIYEKLV"
     gene            13007..13744
                     /locus_tag="VI981_00405"
     CDS             13007..13744
                     /locus_tag="VI981_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEY4478809.1"
                     /translation="MPLVLEHGGYGAIATETKAQGRVDPPVNSFIELLRGYDANCPLG
                     ALGATRMKLNFALIARPGVQISEITKIVAHPKALGACRGRIKALGVEVTEAQSNGKAA
                     EDIAREQELIGAAAIASRIAAEKYGLTILSEAFEDEEAITTFFLLGPTPKHSPLVLGE
                     RNRGLFVFRTKHVAGALVKVLTPFSEEGINLFHIHSLYVGDGEYDFAIETECSERETE
                     MHSRAVTKAREFVIRSILFGPFPVLGV"
     gene            13950..14609
                     /locus_tag="VI981_00410"
     CDS             13950..14609
                     /locus_tag="VI981_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY4478810.1"
                     /translation="MRERVDIFIDGGNFYHLVLKKLNANELDFDFDGFASFLADGRVV
                     AEMGKRFYVGTVRKKVGDLRSKQAMSRQTALFTILKKSQWEIKTSKLKTRIEEIMIDD
                     RVVDYQKIQKLGMQKITIERLREKGIDVKLATDLIVWAIDDKYDTAIVVSSDSDLIPA
                     IDWVRHRGKKKVEYIGFSLLDAKNQENNTTPLLSMISRTDVQRTLVATDLKRFIQQRL
                     I"
     gene            14742..14981
                     /locus_tag="VI981_00415"
     CDS             14742..14981
                     /locus_tag="VI981_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY4478811.1"
                     /translation="MTQKVLKVGSSAAVTIPKKSLKGLGLKIGDEVTVDIDRARKTVS
                     IQPAEKLSHEDVKIAKLTLNFIQRYRTDLERLAQK"
     gene            15418..>15980
                     /locus_tag="VI981_00420"
     CDS             15418..>15980
                     /locus_tag="VI981_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY4478812.1"
                     /translation="MTTEQKRQTLFIGVDVHKDTHTAVGVSSFGEKLFEITVGNYQKD
                     FRLLAEKVETVSAKEMLSPYFGLEDCSGFGERLSNFLLDSGYSVVHVPPILVDERRKN
                     ETHPEKSDSLDALGVAKVMLQRSDTLLPYTVTEESRKAKQIKEISVDREFLVSERTRL
                     KNQLHTLLHRIWNTEYRTKFKDPFSLKA"
BASE COUNT         4243 a         3521 c         3675 g         4541 t
ORIGIN      
        1 cctccgaaac aaccctgcgt tcccctttgc tcgtactcgt tgccgcaacc cttgcatttc
       61 acggttcttc tgaaacgtgt agtggtactt atgatgccgt ccataccccg tacctcctaa
      121 ggaagtttcc gcttatgagt acaggttata tgtaaaaaaa aggaaatggg taattgttta
      181 aagaatacaa agaggtctat ttgactgcga taagaaaggt attatactga ttcgtatggc
      241 agagaagaaa caatcaagaa aaaaagacgc ctacactatc gttttagaac atatccattc
      301 tgattttaag gtatttggtg agggcttgag tctcattcgt gacgaagtga aaggcaatag
      361 tcgtcgtctt gataaagtcg aggagcgact cgcaaacctg gaagttgagg tcgcgcttat
      421 tcgccacaac caagtaactc gcgatgagtt taaatttctc gaaacacgag tagcgcgcct
      481 tgaacggaaa ttaaaatgat aatactgctg gaagattagg gaaatagatg cgagtcctct
      541 cctaatgtaa tgaattgata ttcaaaaagg atgacatata attatagata tggatcaaaa
      601 tactcaaata tttacaagta agaagagtta ttttaaagga gggctttatc tcgggattat
      661 aatcgggatt gtaactggcg tgttttcttt tttgaggttg gataactacg ctcaagtaat
      721 cacgctcgtt ccctttttag cctttctctt atacatttta ggtttcaaca atggcaaggg
      781 cataactgat aaaaattggt tctattttgt atgggggtac ataatcccac ttgcgattgt
      841 ttttcttata agttttccaa ttgcgatgtg gtgtaaatca aacgcaagcg catcaatttg
      901 tttttaagaa gggcttgcaa taagttccaa cttcccgcca gaggcgggca aggctgttca
      961 ctcttttgca cgacaaataa aaaagcgggg gttagcccgc atggttttgt gtatctgcaa
     1021 atgatcttgc tagagcgcga agagtttttg caagggtagt tttggctttc ttttctgcat
     1081 ctctctagct cctttcaaag tgcacagttt atctcaagac tagcaagttg cttgagccta
     1141 tgtcaagatt ccattcaggc tcaaaagttt ccggattcca actacagttt gtcaaaaatt
     1201 tctcaaaata tctcgatatt cctctaaatt tttgatttcg ctccgctcaa aaccaaaacc
     1261 ttttgcctcg caagcaaatt ttgacatagg ctcttcattc tttgctagac tccgggatcc
     1321 cgcttcggct atatgagcag gttgattttt taattgatta gtggtaccat taaaaatgta
     1381 atgagaggga aaagttacga tgcactaaag ctgaatcaaa cggagacgcc ccttttgatt
     1441 tttatggaat catataataa gagtgttcct gccagcttcc ctcatgcgtc agtcaaagta
     1501 ttgaaacaat ttcagatact ccaccccata cttttcaaaa acagcgacga atggtcaata
     1561 gataagcatc gcaaaaagct tatggattgg ctcccttcac atctcgatat tgcataaaca
     1621 tttaaaaatt gcgcgcaatt ttcagtattt cttatactca cccccaaaaa atgttttgag
     1681 tcttcgcagg ccttctttta tttttgagat gtcgagcgcg tagctgaagc gaacacgatc
     1741 gggcgcgccg aaccacgtgc cgtcataggc aatcatttta tatttgtaat agagatcgtt
     1801 cacgacacga tttggatttt taagttttgg gagtacataa aacgcgcctt ctggtttcca
     1861 gagctcgagt cccagatcat gcatgccgcc ataaatcata tctcggcgct cttcccatat
     1921 ttttacgtgg tgcgcgatat atgatcttgg ggatttcatt gcctcgagcg ccattgcttg
     1981 ccccacgatg ctcgtattca tcgaggtatg cgttttcatc tcaataacct tctcgataac
     2041 ctcaatattt tttgaataaa ggtacccgac gcggaatccg cacatcgcgt atgtttttga
     2101 aaatgaattt accgtaacca ctttttctcc ttgaataaga tagtttttcc gcacatagat
     2161 aagatctttg tagacttcgt cggaaattac atagaccccg agcgtgccgc acagcctttc
     2221 gatttttttg agtgttgcga tttcttgcac ggtgccagtt gggtttgagg gggagttgat
     2281 gataaccgcg cggcatccct ggacggcttt tttgaaaatc tcaaaatcaa ttttgccgtt
     2341 tttgtcgagg tcgtagtacg cggggcgcat gccagcgtag agtacgttgt gcgggtacga
     2401 gtagtaatag gggcgcggga gcaggattcg tcctccgggg cgtgataaaa cgcggagcac
     2461 gagatcgagc gcttcggaag caccgtttgt gattacaaaa tgttcgggtt ttgcgtttgg
     2521 gtattcgcgc gcgagtgaag agcgtaagtt ttcctcgccc tggataaggc cgtatttaaa
     2581 ggctttgtag tgcttgagaa tcgaatatgc gcctttgggc ggagggaggt ccggttggcc
     2641 tgaaccaaat gagatcaggc tgtcatcgcc tttgccgata aaaaaagagg ggcttgagat
     2701 tttttggggc ataattgtgc cattatacca tcttgttttc cttttgtggg ggaggaaaaa
     2761 gcaatatgat atatgtatgg taaaaaagcg gatacgccga ggtaagcgct acttcagcgg
     2821 agacgatctc acaaaaaagg ggctcgacag gctccgccgg tcgaaatccg agccgtggga
     2881 gaagaaaaag cgaaaataaa taaacataag tgaattttct tttccctatt gttgccgctc
     2941 tattgcaggc gagttctgca acgctcgata aagttctttt gtcgttcaag cgcgttgact
     3001 acaaagttta taccggtgtg agttacccgc ttctttttgt tattactctc gttattttcc
     3061 tcgttgtccg cccgccgctc tcgcgcgagc ttttccgcga cgatcttttt ttcttgcttt
     3121 taatctcggt tgcctttact acaatcacaa atcttattgt gtaccgcgcg ctcaagcacg
     3181 agcgtcttat cgagattgaa gcggtcgggc ttttacagac tctccccatc atagtattct
     3241 cgagtatcgt atttacagat gagcggcggt atgccgttgt cgtgcctgca gttatcgcgg
     3301 cgtttgcgat tatctggtcg cactgggagc accatcattt tcaaatgaac aaagatacct
     3361 acccgcttct catttggctt ctcgttgccg ctccggtggg cgcagtgctg tcaaaagaac
     3421 tgcttcgcgt gtgggatcct atatcgcttg agcttgtccg ctcgtttctc gtcgcaattt
     3481 tgctttataa gtatttcgag cgagggtttg caaaaatcac gcttcctatt tttgggcttt
     3541 ttgtgcttac caatcttttc tcgtctgtcg cgtggattct cgttttctat ggctatcagc
     3601 gtatcggtat tattcatacg atgcttttgt tctcgctcca gccgatgctt gtctattttg
     3661 catctgcatt ttttcttaaa gaacgcttca gttggaagaa atccgcggct tttatcgttg
     3721 ttcttctctc gattgccaca gcccagcttt taggcgtgta aataggtggt gctggttaag
     3781 aacactttct ctacttgtat tttgccctct gttcctgcaa caataaagga aaaggccggc
     3841 gcatctatgt acaaaatgca aaaggaattt ttatggattc aaaaccaata ggaaccgtaa
     3901 cacactacta cgataaagca ggcgttgcgg tcgttaaatt tgctagtgct gtttcggttg
     3961 gtgataagat aaaaatcgtc aagggagacc atgagtttga acaagagatc tcttcaatgc
     4021 aaattgagca cgagcagatt acgtccgcca aaaagggcag tgaagttgca atcaaagtag
     4081 gcgagcctac aaaagaggga gccgtgatct ataaggttga atagatgctg gtaagctgat
     4141 agtatatatt cgctccgcgc gaatacggtt taagttttat acggtgccta tggtgtagcg
     4201 gtaacacggg agcttgtgga gctcttatca ccggttcgaa tccggttagg cacctgccaa
     4261 ttttactcaa aaagtcgttc cgcaaggtgc ggctttttgg tttggtcttg taagagttct
     4321 cgccttgatg tatgaatggt gtgaatattc tttgaaaaac gaaaaggggg cgatatatgc
     4381 ttcgctatac gttgcgagtc cgggacttgg tacatggcac gattctattt acagaacaag
     4441 aagaaaatat aatcaatcac ccactcttcc aacgtcttcg gcaaattagg caaaatgatg
     4501 tcgccttctt tgtgtacccc tcactcaata cttcacgcct tgagcatgta ttaggtacct
     4561 gtcgagtcgc tggcatgatg gcagaacatc ttactcaaag tcccaagtgg agaacctatc
     4621 tcaaggaact gaaaacagag acctggattg tgtcaaagga gcaattcatc gaactctgcc
     4681 ggctctatgc gcttctccac gacatcggcc atttgccctt aagtcatctt ttcgagatgg
     4741 cggtagagag ttgggcagga aaaccgaaag gagtaattgc ggaggaatgg actgggatcg
     4801 gcggctttac aaaactgcac gaagcattcg gggcctcgat tgtgaagagg atggttccaa
     4861 gtatcaaact ttcgaagccg gtggggagcg cacttctcaa ggtcatgacc gaaaaagact
     4921 taccattcaa acatccgctc tcagtagtga agaagctcgt tgattttgat attgacgccg
     4981 accgcactga tgcaacccag cgggacgggt tctcagctgg cggcgagtac ggtcactatg
     5041 acataaggcg actttgcgat gcggtcttca ttgaacaaga tgatcaagaa tggatcgttt
     5101 cctattctga aaaggccttg acttccatgg aagcactcct tctcgaccgt taccgcatcc
     5161 acctgtgggt tcacttccat catcgcgtag tggcgatgaa gatgcttgtt ggatttctga
     5221 tcgagatagc ccttagacgg aaactcatca ccaaagacca cttcgatcct aagaaagcag
     5281 acgcatttgt cctcaaagac gacgtatggc tctggggcgt gttgcgcgag atgcccacag
     5341 ggggcgaccg aactcttgag atggtcaagc gagctgtctt cttccgcgag aaacgcaata
     5401 ctttcaatct ctggaagaca agacccggtt accacgcact ccgcacgaga gctgcggatg
     5461 gagcaggagt ggctactttc acgattacag acctcgatgc gtattgccgc tatttgagta
     5521 agcgcatgaa aatacgcgtg ttggggttca agccgccctt tgagccgatc agtgataaga
     5581 aagttctgct ctactctgaa gtggagaaga agctgactgg taaagagatc cgcgaagtct
     5641 cacggctcgt tgccggcctc aagacacttt gggagaacga cccgcaggag ttccatctcc
     5701 ttgtgggcaa ggatgcgagg gttcgcagag cacggctaag ggatcgatgg gtggaagaga
     5761 ccgctgactg gatacgctcc ccgagattgt tgtagtatgg agagagatcg ccgaggcccg
     5821 aaaagccaag gaggcaaaag atgaaaaagt tttactaccc tgaccatgac aagcgatatg
     5881 cacatatccc caagagtcag cggcgtttgt accccgacga gatccatata ggctccgccg
     5941 tgatcaagcg aacaaagctt tacaaggaca ttactggcaa ggacatccgg aagtacggag
     6001 ttgacgcttg cctcatcgcc aaggatgtgc cctacacaga gggtctttac gacggctgag
     6061 gatagaaata gttctttcat cccatgggcg gttctaagta ataggccgcc tttttcttta
     6121 tgccagttac ttcttttttc atatcagtat agatatagcg agtagagagg tagggcagat
     6181 tagattttgc ataaatcagg aatataaata tactcatttt gtcgctgaat gatggttcta
     6241 acatcctcca cgatttgacc caagtgtcag aatattttga gaaattgtaa ttctcttgtt
     6301 gatggggact ttgttacttt cgctacgtga gccatgttat acgtgtgaat ggaagtatcg
     6361 tctataaact caaagtttat atttaaagtg ttgtctattg gggccatgct ttttataagc
     6421 tacgcggcta gtctttttgt tcagtctgta gtaaatgacc gaattgcata tcaaacagaa
     6481 gcattaaaaa cacagtcggg agacacttcg cgggttctta tgcacggaaa acttccaggg
     6541 tttgatgaag agggaatcag tacataccgc ctcgtagaca gagtactaaa atatgcaatt
     6601 ttatttattt cgctcacgtt cattgccttt tttcttacag aggttaccca tcaattaaaa
     6661 cttcatccaa tacaatactt gcttatagga ctggggcttg ctgagtttta tctcttactg
     6721 cttgcgttta tggaacatat tggatttctg tgggcgtata ttattgcggc agctatgact
     6781 attttactca tatcgctcta tagttatttt attttaaaga acaaaaaggg cggtgctttg
     6841 atagcaattt tgcttacgtt tatctactct tatcttctcg ccgcgcttaa ccttgaaacc
     6901 tatgctcttc tgtctggcgc acttctcctt tttgttgttc tttctggggt tatgtttatt
     6961 accagaaacc tcaattggta tgatgctttt agttatgtca aaaaagcgga atagatctga
     7021 tttctagatg gttcttgtac tcataaaata acaatagata cccacggttt tacagccgtg
     7081 ggtatctata tacaaataac atttgacaat cttgccagca agatatatga taatatcata
     7141 ttataattta atgctatttt tattatgact acattgtctg ttcccgtgcc ttctcatctg
     7201 gaggagttta tcaacaagca ggtaaggctt ggcaaggctt ccaacaaggc agctgttatt
     7261 cgccgcgcac tccaacttct tgccgaagag gaggccgtgg aggcggtact aaaagcagag
     7321 accgaaccaa cactaagagg taatttgcga gctcttgcaa aaaagctgaa atgattcatg
     7381 tcagctttaa gcctactttt atacgccagt tcaatgtgct tgagggagat ctccagggcg
     7441 aggtagtaga aaaaatagag ttattcaagg atagaaaaaa ccacaaacag ctcaagattc
     7501 acaaactaaa gggacgtctt gctgggcgat atagtttctc ggttaattat cagattagaa
     7561 ttgtatttat ttatgggcaa aaagacgagg ccattttgct agctatcggt agccacgatg
     7621 tgtatagaag ttaatgtttt tactagagct tgtctgaaaa cccctccgcg aaccttttga
     7681 tgggtaggta ggctatgaaa aatggcttaa ccacggcagc acggggctga aaataggctc
     7741 caatggggtt tcctaacagg gtttttgaac atgctctagg tggtgtttag cgttggggta
     7801 taaattgttg catcatccct cctctcaaaa acagttctgg ttcgactgcg ctcaccacaa
     7861 gtagcatctt tatcttgagg ttattgaagg actttgcgat ggcgcccagc taaaaaatca
     7921 ctattctgca acgatatttt ttgaccacca aaacacagtt ggaacttttt gttcttgact
     7981 atcaagctcg cagaccttac tatttatata tgacagtttc taatgcccgc ttcggttttt
     8041 tcttttttac ttcggtaaac cggagggtgg attagtactg taatctcgca acacagtcgc
     8101 cagcccttcg gagctggcga ttttgttctg tgagcgttct ttgacaacaa gaaaggaggg
     8161 gagcgatcat gaaaattacc tacttaggcc cggctggtgc gacgttctcg gcggttgcgt
     8221 ataatgagct ttctcgtctc tttcgcgttc cgtctacgaa tgatgaagga attgagctcg
     8281 tgcttgcaaa aacgaacgat gaaattgact gccccagcct ggaaccccca gaagcaagac
     8341 cgttaaagga ttgttttggc tctttcagga taccaaatta gggcacccta gtcaggcctc
     8401 ggtagacaaa gtagacacta taaaaagggg ctgttttgtg tctaccggcg cctatataaa
     8461 tgttacgcat gtggcacgga aactggagta tcggcaatgt aacaattgtg gcagatttag
     8521 ctctacttta tggactaatt tttcttgctt ttagactgat ttattttcca ccagccgcat
     8581 aaacgccttt tcgtaattat caatcatgcg ttttatccca aaactttctt caatgtgaac
     8641 acgacagttg tttctgttaa tcgcattaac tttttttgtc gcttcaacca tttcttccat
     8701 agtattgaca ataaaaccag ttttcccatc agccacaatt tccgggactg aacctctgca
     8761 aaatgctatc acaggtgtgc ctgttgccat tgattcaatc atgaccagcc caaacggttc
     8821 ttcccactgg atcggtacga gcgttgcttt cgcatttcca taatattcgt aaagttcttt
     8881 tcttgaaacg acgccgatgt gacgaatctt ctctccaaga tatggtttaa cttttttatc
     8941 ccaatactct cctgttaccg gcgggccaag aataatgaga tcgtctccgg ttttttgcgc
     9001 tacttctacc gcttccgcaa ctcctttttc cgggtgaagc cggcctacaa acaataaata
     9061 gtttcctttc ggtttttcat aaaagggaat ttctttggta ttgattccat tataaatcgt
     9121 cgcgagataa ttaaggtctg gggccggtag tcgctgcgaa tcactgattg atacataata
     9181 ttgattcggc gaggagaaca tacgaaaaac ttccgccctc cacttataga tgggatcgtg
     9241 gagcgtatag acggtcggaa cattgcggat tgcgtatgcc aaaggaagcg cgcgatctac
     9301 gggatgaaca tgtataatat caaatttgct cttcaacgct tctttgtaga tggcggagag
     9361 cagaaactga tcccaaaggc cgtagatttt tgccctttcc gctccgccga cattcggtcc
     9421 ggccaaaatg gcaagctccc cgttgtggcg caatggtttc aatccgacgg tttcaatttt
     9481 tgaaactttg attcttgaac cttcgggagc gaagaaggtc acactgtgtc ctcgttttgc
     9541 caatccctcg cttactgcaa ccgcaatatc cattggcgcg taaattacgt ccgctggcgg
     9601 cggagtggcg aagtgtcccg taaccattgt ggcgatgcga agttttctca tttttctaaa
     9661 acaaaattta ataacgcgcc cgaggcccac gcttgaggcg agcacgcgac gggaattcgc
     9721 tctattttgc catccgtcac ggcataaagc tccgggatct cgccaagttc ggaatatgca
     9781 tcaagaagtc cccgcttaat cttttggtat tccttctcgt agccgtactt tttcaatcct
     9841 tgcgcaatga tccagttgtc atgcggccag atcgaaccaa gatgataact cataggattg
     9901 aaatcaaaat ttgactccgc gtgggtgcga ataccgtagg gcgtccgcat atcatcttga
     9961 aaaagccggt ccaccgtcat ttttattttg tcatcgtagc catccaaaat gccggtaaaa
    10021 aggatgtgac cggggtttga cataacgcgt ctgtctgcaa catttgattc ggcaagcgcg
    10081 gatgcaaaaa agttctcatc ggcgagccag aattttttga tgcagttttc ttttactttt
    10141 tccgatcggt ccgcgagaca gcgagcgagt ttcgtatctt taagcataag tgcaagttcc
    10201 gaagcctcgc gcagtgcggc ataataatat ccttgaacct cgataagctc tacgggagaa
    10261 ttgatattca agtgattcaa acggctgtct ttccatcctt gatgcataag gccggtcgaa
    10321 tttttcctct catattcaag aagagtgtct ccgtcaaagt ccccatactt ctcgcaccat
    10381 tcgagtgcgg aaataatatg cggccaataa gccgcgagcc attcggtgtt cacggccttt
    10441 tcgtaataca gaccgcaaag gtatatgaaa agcggcgtcg cgtccactga accatagtaa
    10501 ggaagagccc aggcaagagc tttgtactcc gcggggttcg ggtgccactc atgaacaatc
    10561 ttaccgggtt cttcttcgct agcattgtcg aattttttcc cctgaagcct ggcaaggatt
    10621 ttaatggtgc ggcgagcaat attagggtcg tagtcaatca gttgccagga gacgatgcac
    10681 gaatctcttc cgaaaagggc tgggaatcgt ggaagccccg ctcgtatgta gccgtgttcg
    10741 cgatcttcaa gtttgcccat atccgcttca agcctcgcgg caatttcttt tttggttgtc
    10801 acgcttttcg ttcgcataat tcataataag ttttttcata agcatctgcc atccgttctt
    10861 tgctgaactt ttcctcgata tgtttgcgag cggcggctcg atcgatcgta ttgatttttt
    10921 tcatcgcatc cgtgagcaat tcgatgcttg aatctactac aaaaccggtt tctccatctt
    10981 tgataagctc tttcatcgaa cccctgcgat acgcaatgac tggcgtgccg caagccattg
    11041 cttcggcaac caccaagcca aatggctctt cccattcaat cgggtaaaga agagcttttg
    11101 ctcgaccgta aaaatcggaa agtttgttat cccccaattc cccaacatat ctgatatttt
    11161 ggttgagatg cggcttaata attttttcaa agtattcttc ttgagtcggg tcgattcttc
    11221 cagccagcaa taattttgct ccaaactttt ccgcggcttc aatcgcgttc tccgccccct
    11281 tatgcttatt catccgcgcg atccagataa aatgttcgcc gccttttttg ttgaaacgaa
    11341 aatgttcgag atcaatcccg ttatagatca cacgtccttt cgaaaattca accattgaat
    11401 ttttacgcgc cgactcgctg atgaatacga tatcagtgcg gtgcgcctct tcgccaaaga
    11461 gccttaacct gtcgtctttg gtcgcatccg cgttggtacg gtaaagagga ttgtgcatgg
    11521 tgtgtattat cggcgtttgg acaattcctt gaaaaaacaa tccccaaaga tcgaggtggc
    11581 agtggacaat atcaaatttt ccgtcctcgg ccatctcaaa agcgcgagaa agattccgca
    11641 gattccagat cgggtttgac catgggatat ttgcttccga gagcgatttg tcaaaaacag
    11701 aaataagccg ggcggatgtg gttgatccgg gagatgcaaa gagcgtgact tcgtggcctc
    11761 tccgcatcag ttcttcggtg agcattgcca cgatgcgctc gatcccgccg tatccttttg
    11821 gaggaatagt gaaccagagc ggcgcgactt gagcgatacg aagttttctt ttgccaaatt
    11881 ttttatacaa gcttttcata gattttttca tatttatcga ccatcttttc gagagagaat
    11941 ttttcttcga cggctttgcg gcacgctctt cgatcaatgg ctgaaatgcg ttctattaca
    12001 ttaatcattt cttcatttgt gctcacaaca aaaccagttt tcccgtgttc tataacttcg
    12061 ggtaccgagc cctttctaaa gccgattaca ggggtcccgc aagccattgc ttcaatcatg
    12121 accagaccaa atggctcttg acgatcaatc gggaaaagaa gcgctttggc gtttttcaaa
    12181 agttcaactt tttcctgaaa attcacttgt ccgcgaaagg agatattatc gtcatgaaga
    12241 tgcggctgaa tttcgtgcag gaaaaaagac cattcggcgt ttccggcgct gtttccggca
    12301 ataatcagtt tttgccccgc ggccttggcc acctcaatag cggtcagcac gcccttttct
    12361 cgattgaggc ggcttaaata taaaagatag tcgccggagg aatcattgaa ctcataacgc
    12421 gctggatcga tgccgttata gatcacatca catacgggaa cgccaaacgc ttccatgctc
    12481 cgtttgtgat cttctgagat cgctacatat ttgattttgt gataggcttt catcgtctct
    12541 gcatactgct tgtctactgg ttgatgaaca gtggttagaa atcgcggaca gtcaagtcga
    12601 agcgaaagcg gaaacataaa aaaattgaag tggttgtgga gaacgtcaaa ctcgtagaac
    12661 ctttcaaaaa cctctttcaa catccgcatc accggcgcat gcggatttct caactcgcga
    12721 tcaagccaaa tagctttttc aataacagga acgaattttg cggatgtttt tgagtcgccc
    12781 gaggcgaaaa gcgttatatc gtatccttgt ttcttcagct cttcggtgag ttcggaaagc
    12841 ataagctcaa tcccaccata tgttcgaggc ggtatcggaa tccacaaagg ggcgagttgt
    12901 gcaatgcgag gttttttctt atgcatgaac taattttacc agaaatgaaa caaaaaatac
    12961 cactgtcgtt tagcttaaaa ctattcggtg tgaatccgaa aaacgagtgc cgcttgttct
    13021 cgaacacggt gggtacggcg caatcgcgac ggaaaccaaa gcgcaaggac gagtagatcc
    13081 gcccgtaaac tcttttatcg aattgcttcg gggctatgat gctaattgtc cgttgggggc
    13141 gctaggagca acacgcatga agctcaattt tgcgttaatt gccagaccag gagttcagat
    13201 cagtgaaatc acaaaaattg ttgctcatcc taaagctctc ggagcatgca gaggccgcat
    13261 aaaggcactc ggggttgagg tgacggaagc tcaaagtaat ggtaaggcag cagaagatat
    13321 tgcgcgtgag caggagttaa ttggtgcggc agccattgct tcacgaatag cggcagagaa
    13381 gtacggcctt acgatactat cggaagcgtt cgaagacgaa gaagcaatta caacattttt
    13441 cttactgggg ccaacgccaa aacatagccc gttagtgttg ggggagagaa atagaggcct
    13501 cttcgtattt cggacaaaac atgttgccgg tgcgttagtg aaggtattga cgccattctc
    13561 cgaagaggga atcaatctct ttcatatcca ctctttgtat gtaggcgatg gcgagtacga
    13621 tttcgcgatc gagactgaat gtagcgagcg agagacagag atgcatagca gagcagttac
    13681 taaagcgagg gaatttgtga tacgctccat ccttttcggc ccattccccg tgctgggggt
    13741 gtaaggctca aacctcgagg tactgtatgt actctcgggg ttttttataa acgcattatt
    13801 ctgaaatatg tgccaacata acccattagc caaatggatt attaacagcc ccattgactc
    13861 aattaggttt tttgctagta tacaagtgaa agtcacccgc gcctcctagg agtgtgcggg
    13921 ttttcgtttt tgctctataa tccaaatcaa tgagggagcg agtcgacata tttattgacg
    13981 gtggcaattt ttaccatttg gtactaaaaa agctcaatgc gaacgaactc gattttgatt
    14041 ttgacggatt cgcctcgttt cttgctgacg gcagagttgt ggccgaaatg ggcaagcgtt
    14101 tttatgtcgg cactgttagg aaaaaggttg gagatcttcg ctcaaaacaa gcaatgtctc
    14161 gacaaactgc actttttact atattaaaaa aatctcaatg ggagattaaa acaagcaagc
    14221 tcaaaacgag aatcgaagaa attatgatag atgatcgagt cgttgattat caaaagatac
    14281 aaaaattagg tatgcagaaa attaccatcg aaaggttaag agaaaaagga attgatgtaa
    14341 aactagcaac cgatttaatt gtttgggcga ttgacgataa atatgatact gcaattgtag
    14401 tgagttcgga ttcggatctc atccccgcta ttgattgggt aaggcatcgt ggcaaaaaga
    14461 aagtcgaata tataggcttc tcattacttg atgcaaagaa ccaagaaaac aatacgacgc
    14521 cgctgttatc aatgatttca aggacagacg tacaacgtac tttggtagca acagacctaa
    14581 aacgttttat ccaacagcga ctcatttaat ttcctgcctt aaaatacatt ccaacatccg
    14641 ccttggctcg acgccaagtc gagacgggta atccaccagc cccgacggca ccgtcggggc
    14701 tggtgtttga cagggattat aataagatta taatgaacaa tatgacacaa aaagtattga
    14761 aagtaggttc atctgccgca gttactattc ctaagaaatc actcaagggg ctcgggctca
    14821 aaatcgggga tgaagtgacc gttgatattg accgtgcgcg gaaaaccgtt tctatacaac
    14881 ccgcagaaaa actttctcac gaagatgtaa aaatcgcgaa attaacactc aattttatcc
    14941 agcgttaccg tactgatctc gagcgcttgg cgcaaaaata aagtgaagta tttgagtaag
    15001 gagcagatac tccttatcca ttcaatggtt attgatgaaa cgggtgcaat tatacgacaa
    15061 agcccgaacc tattttgaac gaaactgacc gcctccgctt cgctccggcg gaacgctcgg
    15121 gcgcggcgga attcccccca acctccagta tgagagaatc tactgggatt tttcttggta
    15181 gagtgtagag agttgttgcg gcatttcatt cccacaccct gtcgcctgcc caagttttgc
    15241 ggagcaaaat ttaggcagga acgaccaacg agggattgca ccgcgacaat tctgtgcgcg
    15301 gcaatgaaca tatagcggat ggtcagcctg agcgtagtcg aaggcacatg actctcgcat
    15361 actggggatt cggccgattt cgcacaaaaa tattatcagc gtaatttttg aaaaaaaatg
    15421 acaacggaac aaaaaaggca aaccttgttc atcggcgttg atgtccacaa ggatactcat
    15481 accgccgtcg gcgtttcttc gtttggagaa aaactgtttg aaatcacggt cggcaattat
    15541 caaaaagatt ttcgtttgct tgccgagaag gtggagacgg tatcggcaaa agaaatgcta
    15601 tccccatact tcggtcttga agattgctcg ggtttcggtg aacggctctc caactttctt
    15661 cttgactctg gatactcggt tgtccatgtg cctccgattc tcgtggacga gcgaaggaaa
    15721 aacgaaacgc atccggagaa aagtgattcc ctggacgcgc tcggagtagc caaagtcatg
    15781 ctccagagga gcgacacact actcccttac actgtaaccg aagaatccag gaaagccaag
    15841 cagatcaagg agatatcggt tgaccgcgag tttctcgtct ccgagcggac acggctcaag
    15901 aatcagctcc ataccctgct tcaccgtatt tggaacaccg aatatcggac gaaattcaaa
    15961 gacccgtttt ccttgaaagc
//
LOCUS       DATDSU010000014         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2186157, whole genome shotgun sequence.
ACCESSION   DATDSU010000014 DATDSU010000000
VERSION     DATDSU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186157"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VI981_00425"
     CDS             <1..724
                     /locus_tag="VI981_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY4478813.1"
                     /translation="RHDPKKPDWPERDRLILSNGHICPVMYAAMAHAGYFPKEELKTL
                     RKLGSRLQGHPHRTALPGLETTSGPLGSGLSQAVGMAIGFKMDGSAGSPQVKNRQVYC
                     LMSDGEHQEGQVWEAAMLAGKLKLDNLTAIIDRNNIQIDGMTEDVMPLEPFREKYEAF
                     NWHVLEINGHDMREIVNAVETARAVYEKPVAIIAHTIPGKGVDFMEFKYQWHSKTFTP
                     EEAKESLRQLRTLGGKIRSEHE"
     gene            1034..1684
                     /locus_tag="VI981_00430"
     CDS             1034..1684
                     /locus_tag="VI981_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478814.1"
                     /translation="MESVFSRLNQRSVLTFLLLTVIAGAVSFFLLKKADTTINEIESI
                     QSAPLFVARKEYLLDDTGIIENVSGWKTYRNEKYGFEVKYPADFEEYKSSREFGASQS
                     SAYMEVFVNIIPPSVNFREYLDALTRVRAKGQRDGRIVIFKKVSKAVSGREGFEEELY
                     DSSINSFVIRTFADFTPLAIVSITVREGGKLEGKTGVSSGLRRVSDGITSTLRRVP"
     gene            1855..2421
                     /locus_tag="VI981_00435"
     CDS             1855..2421
                     /locus_tag="VI981_00435"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEY4478815.1"
                     /translation="MTEQEKLLGDVGRILDNLDIRYVVTGGIAVTVWGRPRFTADIDI
                     AIELLPKKVDQLASALLKIDKDVYVDREMMRHALIEKGEFNFINPASGLKVDFFVLKG
                     SRFDKEEIARCVRKKIAEVWVSLISPEDLVIRKLLWHRESGSDRQLEDVESILKRQKK
                     LDRGYLKKWAKYHSVFALLETLWKKTKR"
     gene            2400..3050
                     /locus_tag="VI981_00440"
     CDS             2400..3050
                     /locus_tag="VI981_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478816.1"
                     /translation="MEKDQKINGFGLLGIIIVVAIVAAFSIGGGMYWNETKKQQSLLQ
                     MGADAKKRAEELKQAIESRNEQIFGSPTSEDTDVNTFEWKTYRNEKYGFEVKIPPRWS
                     AADIEEIKDAYYGGINIFLRLPSKNSGLQPVCHFSVYQSSQWDRLQKDVETNKPIFLV
                     SKDDTLYGWGCGHDDYGYRGFEEYNQAVDDQQLKDLPMGPFQEFKKSILPTFRLIK"
BASE COUNT          991 a          621 c          843 g          842 t
ORIGIN      
        1 gcggcacgac cccaaaaagc cggattggcc agagcgtgat cgccttattc tttcaaacgg
       61 ccatatttgt cccgtgatgt atgcggccat ggcgcacgca ggatattttc caaaagaaga
      121 attgaaaact ttgagaaaat tgggctcgcg ccttcagggg catcctcacc gcaccgcgct
      181 tcccgggctt gaaacaacat ctgggccttt gggctccggt ctttcgcaag cggttggaat
      241 ggcgattggg ttcaaaatgg atggttcggc aggctcacca caagtaaaaa accgccaagt
      301 gtactgcctt atgtcagacg gtgaacacca agaagggcag gtgtgggagg cggcgatgct
      361 tgccgggaaa ttaaaacttg ataatctcac tgccatcata gatagaaata acatccagat
      421 tgacggaatg accgaagacg tgatgccttt agaaccgttt cgagaaaaat atgaagcgtt
      481 caattggcat gttcttgaaa taaacggtca tgatatgagg gaaatcgtaa acgcggttga
      541 gacagcgcgc gcggtctatg aaaagccggt tgcgataatc gcgcatacga ttccaggaaa
      601 gggagttgat tttatggaat tcaaatacca gtggcattca aaaacattta cgccagaaga
      661 agcgaaagag tcgcttcgcc agctccgaac cctcggaggg aaaattcgaa gcgagcatga
      721 gtaagggctc gctgatacgc tttctcgaca cgtaaatttt ttccagcgaa aaaattcact
      781 aaggggtcgc aaaatttttc cccgatgata cgtcggggaa accatgcaaa ttttgccgac
      841 cattgtcgag aaaacatatc agctcgcaag agcatgaaag caaaaagagg ttgtttcaaa
      901 agcatattgg ctcgctgaag catgaactga atgtcgttcc tccattgtct gaaaagaatt
      961 tatctcaacc gtctaccgac tcgccgaagc gtgagatata aatgtgtaaa ttgttagcaa
     1021 gtaaaatttt cttgtggaat cggtattttc acgattgaac cagcgatcag tattgacatt
     1081 tttgctcctc accgtcattg caggggcggt ttcgtttttt ctcttgaaaa aagccgatac
     1141 tacgataaat gaaatcgagt caatccagtc ggcgccattg tttgtcgcgc ggaaggaata
     1201 ccttttagat gataccggga ttatcgaaaa tgtatcaggt tggaagacat atcggaatga
     1261 aaagtacgga tttgaagtaa agtatcctgc agattttgag gaatataaat cgtcgaggga
     1321 gttcggagct tcgcagtcga gtgcatatat ggaagtattc gtaaacatta ttcctccgag
     1381 cgtaaatttt cgtgagtatc ttgacgcgct cacacgcgtt cgggcgaaag gacagcgcga
     1441 cggccgcata gtaattttta aaaaagtgtc gaaagcggtt tccggacgag agggatttga
     1501 agaggaattg tatgattctt cgattaattc ttttgtgata cggacgtttg cggacttcac
     1561 gccgctcgcg attgtatcga ttactgttcg tgaaggcgga aaactcgaag ggaaaaccgg
     1621 agtgtcgtcg ggcttgcggc gcgtaagcga cggaatcact tcgacgctgc ggcgtgtgcc
     1681 atgagagcat gaaaaagaaa ttcaggagaa atatgacggg caggggcttt tcgggaaaaa
     1741 cagccgagga tattcaggat aggatttttc agaagatgtc agccgaggag agtatacgtt
     1801 tttctggcaa actttatcaa tttggtcgta tgctgaataa actaggagat cactatgacg
     1861 gaacaagaaa aactgcttgg agacgtcgga agaatacttg ataacctcga tatccgctat
     1921 gtcgtaaccg ggggcatagc ggtaacggtc tgggggcggc cgcgtttcac cgcagatatt
     1981 gatattgcaa ttgagctttt accgaaaaaa gttgatcaat tagcaagcgc gctcttaaaa
     2041 atcgataagg atgtatatgt cgatagggaa atgatgaggc atgcgttgat cgagaagggc
     2101 gaattcaact ttatcaatcc tgcaagcggg ctcaaggtgg atttttttgt tttgaaaggg
     2161 agtcggtttg ataaggaaga gatcgcacgg tgcgtaagaa aaaaaattgc agaagtttgg
     2221 gtgtcactca tttctcccga ggatcttgtt attcgaaagc tactctggca tagagaaagc
     2281 ggttcagacc gacaactcga ggatgtcgag tcgatattga aaagacaaaa aaaactagat
     2341 cgtgggtatc ttaaaaaatg ggcaaaatat cattccgtat ttgccttatt ggagacgtta
     2401 tggaaaaaga ccaaaagata aatggattcg gacttctcgg aataataatt gtagttgcga
     2461 ttgttgccgc attcagtatc ggcggcggaa tgtattggaa tgagacgaaa aaacagcaat
     2521 cgcttttgca aatgggggcg gatgcaaaga aacgcgctga agaattgaaa caagcgatcg
     2581 aatcccgcaa cgaacaaatc ttcggaagtc cgacttccga agatacggat gttaacactt
     2641 tcgaatggaa aacatatcgg aatgagaagt atgggtttga ggtgaagata cctccccggt
     2701 ggtctgccgc tgatattgaa gaaataaaag acgcatatta cggcggaata aatatctttt
     2761 tgcgattacc atccaagaat tccggtcttc agcccgtatg ccacttttct gtttatcaat
     2821 ctagtcagtg ggatcgtctt caaaaagatg tagaaaccaa caagccgatt tttttggtga
     2881 gcaaagatga cactttgtat ggctggggat gcggtcatga tgactatggc tatcgcggat
     2941 ttgaagagta taatcaggcg gtagatgatc agcaacttaa agatcttccg atgggtccct
     3001 ttcaagaatt taaaaagtca attctgccca cttttcgact cattaaataa tttttcatgc
     3061 cgatatatcc cggggcaaaa attgttgaac attggtcgga tttaggaacg attgatcaaa
     3121 agccgactcg gaacgagtat ggagatggtt tggttgaatt ggggaagaaa aatccgaatg
     3181 tcgtggtttt gtgcgcggat ttgacatata tacatcgcga tgtataattg aattatatga
     3241 atcgaaccca aatttatttg cctcaaaagc aaattgaaat attgcgaaaa gaggcgc
//
LOCUS       DATDSU010000015         7240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_513803, whole genome shotgun sequence.
ACCESSION   DATDSU010000015 DATDSU010000000
VERSION     DATDSU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513803"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VI981_00445"
     CDS             <1..1273
                     /locus_tag="VI981_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY4478817.1"
                     /translation="SGPLYSKALIEGILDTGVNVIDIGMTLVGTFYWSQHYLKQRGGA
                     YISASHNPPEYNGIKFANDYSETLVSDGIQELKRMVEEEDYERASQKGTLQSQDINPV
                     YIEDVSKRFAINKKLRVVVDPSNSTAGTLAPKILRAVGCEVIEHNCTVDPSFPLGVAD
                     PTSSVIAKRLAREVTDAKADVGFSYDADGDRIGIVDEKGNIIWNDVLLAIFAIDVLRE
                     HPGSTIMYNILCSKVVEETIVEHGGKPFVWRVGHSFLKKKNQEVKAAFIGELSGHFFF
                     SKDFYNHDDGLYSTLRLIRFISGSGEPLSESVERLPHYISSPEIKIYCPDTAKVSTVA
                     KISPELKKRYPDAVILDGESAGDGIRLDFDDGMIVVRYSQNGPYLTIKYEAKTAERYD
                     KFRNIIKELLKKYPEIDWSSKINLNLEALEN"
     gene            1442..1738
                     /locus_tag="VI981_00450"
     CDS             1442..1738
                     /locus_tag="VI981_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478818.1"
                     /translation="MSHRNREATERDEFAKRSAAKQARMNHPSGKSKYARKAKNGGSA
                     IWEDHPDKEPPLPPQANQYDPFASTESELEYIRSLRERRRQSTGSDRHYFGFGY"
     gene            complement(1866..2414)
                     /locus_tag="VI981_00455"
     CDS             complement(1866..2414)
                     /locus_tag="VI981_00455"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY4478819.1"
                     /translation="MTISQKIKSGQIGVMPTDTIYGIVGSALMSHVVERIYRVRKRDP
                     KKPFIVLISKTEELSQFGVTVDLETSKLLNSVWPGPTSVILPCHNAEFEYLHRGTKTI
                     AFRLPNENDLIELILETGPLVAPSANLEGALPAETIDEAKRYFGNKIDFYVDGGILSG
                     APSKIAKIENSKLVYPVRSRDR"
     gene            complement(2411..3421)
                     /locus_tag="VI981_00460"
     CDS             complement(2411..3421)
                     /locus_tag="VI981_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478820.1"
                     /translation="MEEMYIQHKGRIVNADSQFTHVMAIAIDPRTQYREGVIRCITSR
                     EGDVAVKGYIDRSELHKITGNSLESFEIGEKLDIRNESEILNELKGQELDFIGLEDPD
                     IWIDEQNNLLHLYFTLPLINTSDHDRNKIHLGHAVGQNLSSLEMKTPVLFADAIGGAK
                     ELSVAPVNMRGVRLNLVESSKKEIDFTYSVVRIAIAEGMDGPWKFGDIVFHPKEQAIP
                     WIAGHASPGPLFSQNFMDIGKGKRVGLINGREANEKTESDTKYGIFSVGLFIYDYENG
                     KIEWVSPEPFIRDSEAKTITFASQFVETKLGEGILFAHVDDSFVRAYTLNADGIQRLL
                     PS"
     gene            complement(3524..4771)
                     /gene="eno"
                     /locus_tag="VI981_00465"
     CDS             complement(3524..4771)
                     /gene="eno"
                     /locus_tag="VI981_00465"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY4478821.1"
                     /translation="MSKIKQLKASEIIDSRGNPTVEVVCELRDGTISRASVPSGASTG
                     TNEAVELRDHDQKRYNGLGVLTAVNNVNTEINSHIKEREFNQQTLDQALIALDGTENK
                     SRLGANAILGVSLAFARAAAKEQGIELYTYLGNLVGNNNFRLPQPMFNIINGGKHADS
                     GLDVQEFMICPLGVNTFHEKIRVGAEIITSLRKILKGKGYVVSVGDEGGFAPKLPSSE
                     HAFEVIIQAIKEAGYSVDAVKIGIDVAASSFYRDGSYKLKISGQEKSMNSSDMINWYK
                     TLIDTYPIVSIEDGLAEEDWEGFRKMIDVLGDEIKVVGDDLTVTNIKRIVTAIELQAV
                     NSVIIKPNQIGTLAETVRAIEATKKQGWIPFVSHRSGETTDTFIADLAVGLSCDFIKS
                     GSITRGERVCKYNRLMEIENQIL"
     gene            complement(4788..5588)
                     /locus_tag="VI981_00470"
     CDS             complement(4788..5588)
                     /locus_tag="VI981_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY4478822.1"
                     /translation="MISKAQIVATIGPSCADVSVLSEMIAHQMDVARLNLFWGSFDEH
                     AKYIEAVREAEKKSGRKIVLMLDLPGPRLQTGGTHTYDPNTLSVLTDEDKECIKFGVD
                     RDVDYFAESFVGDEKDILHCREVIKGCGGSQRIVAKIERSKAIENLAEIIKVADAIMV
                     ARGDMGSEVPLERIPFVQDLIVRTSKAAAKPVIVATEMLFSMVESPRPTRADVTDVAH
                     AILQGADAVMLSEETAKGKYPIEVVTMMEKIVVEAERHMNGKETINPL"
     gene            complement(5625..7154)
                     /gene="gpmI"
                     /locus_tag="VI981_00475"
     CDS             complement(5625..7154)
                     /gene="gpmI"
                     /locus_tag="VI981_00475"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY4478823.1"
                     /translation="MDKKIIALIVLDGWGYREETANNAIASARKPFFDSMWNKYPHAL
                     LEASEESVGLPKSQMGDSETGHRVMGAGRVPKSDLVRISDASKSGVFDKNQSFQRLFE
                     HVNKNNSVLHVEGLLGPGGIHSHTDHLYAFLRTAKAAGVKKIAVHVFTDGHDTPPQSA
                     GRYIVELQKFLDELDVGFIATITGRYFAMDRDNNWDRVKQAEDAIFECKGNVCHLKEG
                     DLLIDQLYSGKDVDDNLISPIVCLDEKNQACGIKENDGVFFFNFRPDRARMLSAIISE
                     RAMNSNIYFVTMTQYDTTLKTGVAFPPEMIMHTLGEEISNHGSTQAHIAESEKFAHAT
                     YFLNGGREKPYPGEEDVLAPSIKEINGRPIHNYDEVPEMQSVGVADEALKQIEKGTDF
                     VFINFANADMVGHTGNVAAIVKAVEAVDMALARVVFALLAKGGIALITADHGNAEINI
                     DPVSGLKHTAHTTNPVPVILTAKNVRLKNGSLANVAPTILSLLGIEKPPEMTADSLIV
                     K"
BASE COUNT         1935 a         1760 c         1499 g         2046 t
ORIGIN      
        1 gtcaggcccg ctctattcga aagccctgat cgaaggcatc ctcgatacag gagtcaacgt
       61 cattgatatc ggaatgacgc tagttggcac gttttattgg agccaacatt atcttaagca
      121 acgcggaggg gcgtacattt cggcctcgca taacccgccg gaatataacg gtattaaatt
      181 cgccaatgat tattccgaga cactcgtgag tgacggtatc caagaactta agcggatggt
      241 tgaggaagag gattatgaac gcgcctcgca aaaaggcaca cttcaatcac aagacattaa
      301 cccagtatat atagaggatg tgtcgaaacg ctttgccata aataaaaaat tgcgggtagt
      361 cgtcgaccca agcaacagca ctgcagggac gctggcgcca aaaattttac gggctgtcgg
      421 ttgcgaagtg attgaacaca attgcacagt cgatccgtcc ttcccgctcg gcgttgcaga
      481 tccaacatct tcggtaatcg cgaagcgtct ggcacgggaa gttacagacg ccaaagccga
      541 tgtcggattc tcctacgatg ccgacggcga tcgcataggc atcgttgatg aaaaaggaaa
      601 tattatttgg aatgacgtcc tgctagctat ttttgcgatc gatgtgcttc gagaacatcc
      661 gggttcgaca atcatgtata acatcctctg ttcaaaagta gtggaagaaa ccatcgttga
      721 gcacggcggc aagccatttg tgtggcgagt cggacattct tttctcaaga aaaaaaatca
      781 ggaggtgaaa gcggccttta tcggtgaact ttcagggcac tttttctttt ccaaagattt
      841 ttacaaccac gatgacggac tgtattccac gcttcggctc attcgtttca tctctggttc
      901 cggtgagccg cttagtgaat cggtggagag actgccgcac tacatctcaa gtcctgaaat
      961 aaaaatttat tgcccggata ctgcaaaagt ttccacagtc gcgaagataa gtcccgaact
     1021 taaaaaaaga taccccgacg cagtcattct cgacggcgag agcgcaggcg atggaattag
     1081 acttgatttt gacgatggta tgattgtcgt tcggtattcg caaaacggtc cgtacctaac
     1141 tataaaatac gaagcaaaaa cagcagaacg gtatgacaaa ttcagaaata taatcaaaga
     1201 attgctgaaa aaatatcccg aaattgattg gtcatcaaaa ataaacctaa atcttgaggc
     1261 cttggaaaac tgacgactcg tcgcacattc taggcatgaa cgcaatcaca attttcgcgc
     1321 ttccttttct tgcataggca gagatttttg gtcgtaaaaa taagcctcaa atttcttgat
     1381 atttgaaagg ctttttgata tcgtggcgca agcaacggag aacaaccaac cgcacactcc
     1441 tatgagccat cgaaaccgtg aagcgacaga gagagacgaa tttgctaagc gctcagccgc
     1501 aaagcaagca cgaatgaacc atccgagcgg caaaagcaag tacgccagga aagccaaaaa
     1561 cggtggtagt gccatctggg aagatcatcc tgataaagaa ccgcctttgc cgcctcaggc
     1621 gaaccaatat gatccctttg cgagcacgga aagtgagctc gagtatatcc gtagtttgag
     1681 agagcggcga cggcaaagca ccggtagcga ccggcattac tttggcttcg gatattaaca
     1741 aggtgaaatc acatctcgcc tttttttatc gcaagtagta aaccccgtta gaagctcaca
     1801 cagggcatta aatcccgtgt tcgcaaaaat caaattgcat tcatcccgct agaagcaggt
     1861 cgcgatcagc gatcgcggct tctaacgggg taaactaatt ttgagttttc aatttttgcg
     1921 atttttgaag gcgcgccaga cagaattcct ccgtctacat aaaaatcaat tttattgcca
     1981 aaatatctct ttgcctcatc gattgtctct gcaggtagtg caccttcaag attcgccgag
     2041 ggcgcgacaa gcggccccgt ctcgagaatc aattcgatta aatcattttc gttgggcagt
     2101 cgaaaagcaa ttgtcttcgt gcctcgatgc aagtactcaa actccgcgtt atgacagggc
     2161 aagatgacgc ttgtcggacc cggccagacg gaatttaaaa gtttggacgt ttccaagtcc
     2221 acagtcacac caaactgact aagctcttca gttttagaaa ttaaaacgat aaaaggtttt
     2281 tttggatctc tttttcgaac tcgatatatt cgttcaacta catgagacat gagtgccgac
     2341 ccgacaatac cgtatatcgt gtcagtcggc ataactccaa tctgaccaga ctttattttt
     2401 tgcgaaattg tcatgaaggc aaaaggcgct gaattccgtc tgcgttaagc gtataagccc
     2461 taacaaaaga atcatccaca tgagcaaata atatcccttc accgagtttc gtctcaacga
     2521 attgactggc aaatgttatg gtttttgcct cactgtcgcg aataaagggt tccggtgaaa
     2581 cccattctat cttgccattt tcataatcat aaataaataa tccaactgaa aaaattccgt
     2641 attttgtgtc gctttcagtc ttctcgtttg cttctcgtcc atttatgagt ccgactcttt
     2701 ttccttttcc aatgtccata aaattttgag agaacagagg ccccggcgag gcatggccag
     2761 ctatccacgg aatcgcctgt tctttaggat gaaaaacaat atcgccgaac ttccatggcc
     2821 catccatacc ttcagcgatg gcaattctta caacggaata tgtaaaatca atttcttttt
     2881 tactcgactc taccaaattg agacgaaccc ctctcatgtt tacgggggca accgataact
     2941 ccttggctcc gcctatggca tccgcaaaaa gcaccggcgt tttcatctcc agagaactca
     3001 aattttgtcc cacggcatga ccgaggtgaa ttttatttct atcgtgatct gatgtgttga
     3061 ttaacggcag ggtaaaatac aaatgcagta aattattttg ctcatctatc caaatatcag
     3121 ggtcttccaa gcctataaaa tctaattctt gtcctttgag ttcgtttaat atttcagatt
     3181 cattcctgat atctaacttt tctccgattt caaaactttc caatgaattg ccggtaattt
     3241 tatgaagctc acttctatca atatagccct tcacagctac gtctccttcc cgacttgtaa
     3301 tacaccgaat cactccctct cgatattgag ttctcgggtc aatggcaata gccataacgt
     3361 gagtaaattg gctatcggcg tttactatcc ggcctttgtg ctgaatatac atctcttcca
     3421 tatgaaaaag tataccaggc tagaatgact ttcacagtct ctttaaaaac gggccagctc
     3481 gttttggggg ttggcacgta ttgctggcgt gtgtgaagtg gctctataag atctgattct
     3541 caatctccat taatctatta tatttacaaa ctctctcacc tcttgtaatt gaaccggact
     3601 taataaaatc gcatgaaagc cctacagcaa gatcggctat aaatgtatct gttgtctccc
     3661 cagatctgtg agagacaaat gggatccaac cctgtttctt ggtcgcctct attgctcgca
     3721 ccgtttcggc aagtgtaccg atttgattgg gcttgatgat aactgaattt accgcctgca
     3781 attcgatcgc cgtcacaatt cgtttgatat ttgtgaccgt taaatcatcg ccgacaactt
     3841 ttatttcatc ccctaatacg tctatcattt ttctgaaacc ctcccagtct tcctcggcaa
     3901 gaccgtcctc gatggacaca atagggtagg tatcaatcaa tgtcttatac caattaatca
     3961 tatcggaact attcatactt ttttcctgcc cagaaatttt gagtttatat gaaccgtcac
     4021 gataaaaact tgaagcggct acatctatgc ctattttcac cgcatccact gaatatcctg
     4081 cttctttaat agcttggatt atgacttcaa acgcgtgctc gctcgaagga agtttcggcg
     4141 cgaaaccgcc ttcatcacca acacttacta cgtacccttt gcctttaaga atttttctca
     4201 atgaggtgat aatttccgct cccactcgaa ttttttcgtg aaaagtattg acacctaacg
     4261 ggcaaatcat gaactcctga acgtccaaac cgctatcggc atgtttacct ccgtttatga
     4321 tattgaacat tggttgaggc aaacgaaaat tattgttgcc gactaaattg cctaaatagg
     4381 tataaagttc tattccttgt tcttttgccg cagcccgcgc gaacgccaaa gacacgccga
     4441 gaatagcgtt tgcgcccagc cgagatttgt tttccgtgcc gtcaagcgcg atcaaagctt
     4501 gatccagagt ctgttgattg aattcccttt ctttaatatg tgaattgatt tcggtattaa
     4561 cattgttaac cgcagtaagc acccctaatc cgttgtagcg tttttgatcg tggtctcgga
     4621 gttcaaccgc ttcattggtt ccggtactgg cgccagacgg caccgacgca cgagatatgg
     4681 tgccgtctcg aagttcgcag acaacttcca ctgtcggatt accgcgagaa tctataatct
     4741 cacttgcttt taactgtttt atttttgaca ttcctgacat agtgttctta tagaggattg
     4801 attgtctctt ttccgttcat atgacgctcg gcttcaacca caattttttc catcatagtg
     4861 acgacctcta ttggatactt ccctttggcc gtctcttcag aaagcatcac ggcgtccgcg
     4921 ccctgtagaa ttgcgtgggc aacgtcggta acatctgccc gcgtcggccg cggactttca
     4981 accattgaaa agagcatttc cgtggcgacg attacgggct tcgcggccgc tttggacgtt
     5041 cggacaatca gatcctgaac aaaaggaatc ctttcaagcg gaacttcgct tcccatatct
     5101 cctcgagcaa ccatgatcgc atctgccact tttataatct cggccagatt ttctatcgcc
     5161 ttactgcgct ctattttcgc gacaatgcgc tgactgccac cgcacccttt tatcacttcc
     5221 cgacaatgca gtatatcttt ttcgtctccg acaaatgatt cggcaaaata atcaacatcg
     5281 cggtcaactc cgaattttat gcactcttta tcttcatcgg taagaacaga aagcgtgttt
     5341 ggatcatagg tgtgagtacc gccagtttgc aggcgcggcc ccggcaaatc aagcatgagt
     5401 acgattttcc tgccggattt tttttcggct tccctcacgg cctcgatata tttcgcatgc
     5461 tcgtcaaaac tcccccaaaa taagttgaga cgagccacgt ccatttgatg agcaatcatt
     5521 tccgagagca ctgaaacatc ggcgcaagac ggtccgatgg ttgctactat ttgggctttt
     5581 gaaatcataa attttctgtc tcaaaattca taatgagttg tgctctattt tactatcaaa
     5641 ctgtcggctg tcatttctgg cggtttctct attccaagta gagataaaat agtcggcgcc
     5701 acattcgcca aactgccatt tttcagccgg acgttcttcg cagtcaagat tactggaaca
     5761 ggattcgttg tgtgcgctgt atgtttcaaa ccgctcacag ggtcaatatt tatttctgca
     5821 ttgccgtggt ctgcagtaat aagggcaatc ccaccttttg caagaagagc gaaaacaaca
     5881 cgtgcaagag ccatgtcaac cgcctcaacg gcctttacaa tcgccgccac attacccgtg
     5941 tgtccgacca tgtcggcgtt ggcgaaatta ataaaaacaa aatcagttcc cttttcgatt
     6001 tgtttcaggg cctcgtctgc cacgccgact gactgcatct ctggcacttc gtcataatta
     6061 tgtatcggcc tgccgtttat ttcttttata ctcggcgcca aaacatcctc ttcgcctgga
     6121 tacggtttct cgcgcccgcc atttaaaaaa taggtcgcgt gtgcaaactt ttccgattcg
     6181 gctatatgtg cttgagtcga accgtggttt gaaatctcct cacctagcgt gtgcataatc
     6241 atctcgggtg gaaacgccac accggtcttt agagtggtgt catactgcgt catcgtcacg
     6301 aaatatatat tgctgttcat ggcgcgctcg gaaatgatgg ccgaaagcat gcgtgctctg
     6361 tcgggacgaa agttgaagaa aaaaacaccg tcgttttctt ttatgccaca ggcttgattt
     6421 ttttcatcaa ggcacactat gggcgatatg agattatcgt caacgtcttt accactatat
     6481 aactggtcaa tgagtaaatc tccttcttta aggtggcaga catttccttt acactcaaaa
     6541 atggcgtctt cggcctgttt gactctatcc caattattgt cccgatccat ggcgaaatat
     6601 ctgcccgtta tggtggcgat gaaaccaaca tcgagttcat ctaaaaactt ctgcagctca
     6661 acaatatatc tgccggccga ttgcggaggc gtgtcgtgac cgtctgtaaa aacgtgaaca
     6721 gcaatttttt taacgccagc cgctttggca gtgcgaagaa aggcatacaa atgatcggtg
     6781 tgactatgga ttccgccagg gccaagcagt ccctcaacat gaagaacgga attgttctta
     6841 ttgacatgtt caaaaagcct ctgaaaactt tggtttttgt caaaaacacc gctttttgac
     6901 gcatcggaaa ttctgacaag atcgcttttt ggaactctgc cagcgcccat cactcgatgt
     6961 ccagtttcgc tgtcacccat ttgacttttt ggcaaaccaa cagactcctc gcttgcctca
     7021 agcagagcat ggggatattt attccacatt gaatcaaaaa atggctttcg ggccgatgcg
     7081 atggcgttgt ttgccgtctc ctcacggtac ccccacccat cgagcacaat gagagcaata
     7141 atttttttgt ccattggcta tatgatagca gttatccagt gcatctttaa agcaggtacc
     7201 aatctgggca cacaaggtta ctctttatct caagaaaacg
//
LOCUS       DATDSU010000016         4302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_846102, whole genome shotgun sequence.
ACCESSION   DATDSU010000016 DATDSU010000000
VERSION     DATDSU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846102"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VI981_00480"
     CDS             complement(<1..208)
                     /locus_tag="VI981_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478824.1"
                     /translation="MAYSEKIIPVLHSLRPLIMPGWGNAAIINKKDDLAVSVVTAIDI
                     EIEKRTRDALSKLFPKIGFVGEECG"
     gene            complement(526..1368)
                     /locus_tag="VI981_00485"
     CDS             complement(526..1368)
                     /locus_tag="VI981_00485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4478825.1"
                     /translation="MIDTHELREQQRQAWNKFSSGWKKWDEFFMAFFRPIGEKLIEVA
                     DLTDGYQVLDVSTGTGEPGLTAASHIPKEKVVGTDIAEDMIKLANLHAHERVIKNYEA
                     IALNDERQPFPDNSFGAVMCRFGIMYFPSMSEGIREMVRVLKPDHKIALSAWADAAKN
                     PWATTIRGVINSMLSIPVPPPDAPTPFRCAKFGTLSKLFEDAGLKGVQELEIKGQVAF
                     NSPEHYWDVMTAAAPPLVQALSKANEIKRSQIQQAVFEAARKYESRGKIVFNWSSWVA
                     VGVK"
     gene            complement(1624..1710)
                     /locus_tag="VI981_00490"
     tRNA            complement(1624..1710)
                     /locus_tag="VI981_00490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1675..1677),aa:Ser,seq:gct)
     gene            1794..2114
                     /locus_tag="VI981_00495"
     CDS             1794..2114
                     /locus_tag="VI981_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEY4478826.1"
                     /translation="MSQTYKIRTKVWLYPGMNSTGSPQSGRWHFASIDKKISVKIKEG
                     WGNRKRRGWGSVPVKVTLGKSSWKTSIFPDKNSTYLLPIKKEVRKKEAIFDGDSVTFR
                     IEIK"
     gene            2312..2584
                     /locus_tag="VI981_00500"
     CDS             2312..2584
                     /locus_tag="VI981_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY4478827.1"
                     /translation="MSVNFRKTERIIKGVANHRRLKILDLLRREPELSVDEIADKTKC
                     GYMNASDHIRKLALAGLVMKRSDSTSVRHKLTALGEHILVFCKKLE"
     gene            2632..3144
                     /locus_tag="VI981_00505"
     CDS             2632..3144
                     /locus_tag="VI981_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478828.1"
                     /translation="MEELELTYLAKYLPKDLEKFLSKEILDIYIPESSNHPTIRIRKS
                     GAKYEITKKEPINAHDLSRQLEQTIPLTEAEYKDFEKLCGKRLSKRRYFYKTHNTAHE
                     IDVFTGDLAGLVVVDVEFSTEAEKASFAMPDFCLVDVTQEKFIAGGMLCGKTYKDIEP
                     NLEKFGYKKI"
     gene            3258..>4302
                     /locus_tag="VI981_00510"
     CDS             3258..>4302
                     /locus_tag="VI981_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478829.1"
                     /translation="MTTIHELPFRKSKIRSTVEATLEATGGLLRLAPTWVPRSFLQPG
                     LRLKLHPKDTYAYGLNRGGIDERWFASTTPAANENRTRHEGLSFVVADRECFMLAQAV
                     EECGAMLIGKKIWGKYKKWPVYSKFFDNMGPIPHHMHQSRKQANLVGQEGKPESYYFP
                     PQHNNVGNNFPHTFMGLEPGTTREQVRRCLENWNSGDNGILDLSKAYRLKVGSGWLID
                     PCILHAPGSLCTYEPQWGSDVFGMYQNLVEGREVPWSLLVKDMPKSKHQDFDFIVEQL
                     DWDKNVDPKFKEHHYLEPVPVSGSTNEDHTDLWIVYGTIDGEQLFTAKELTVEPGRTV
                     TVWDRGAYGLICVQ"
BASE COUNT         1188 a         1021 c         1007 g         1086 t
ORIGIN      
        1 cgccgcactc ttcaccgacg aatcctattt tagggaagag tttcgagaga gcgtctcgcg
       61 ttcgtttttc gatttcaata tcgatagcgg tgacaacgct tacggctaaa tcgtcttttt
      121 tgttgataat ggccgcattg ccccagccgg gcattataag cggccgaagt gagtgcaaaa
      181 cggggatgat tttctcggaa taggccattg attatcagta tacaaaagtt agcgaaaaag
      241 taatcagtgt ggcaaagcgg tatcgcgcta aaggtggaga aggaaaatgg gcgtgcaggc
      301 ggtgaagaaa aaatcagtcg cgcgtaaaaa gagaatttcg gataacattt tcctgcgggg
      361 cacacgagcg gaacaagcgg cggctccttt cgacaagctc aggacgagac tgaaacgcga
      421 ccattaataa cctgccccac tggagtcatt cagacgggtt tcaagcctgt cccgtagtga
      481 gtctgtctct actaccgggg tttttccact gatagtccct ttttgctatt tgacgcctac
      541 tgccacccaa ctgctccagt taaagacaat tttacctcgg ctctcatatt ttctagccgc
      601 ctcaaatact gcctgttgta tctggctgcg ttttatctcg ttcgcttttg atagagcctg
      661 aacaagtggc ggcgctgcgg cagtcattac atcccagtaa tgttccggtg aattaaaagc
      721 aacttgccct ttaatctcca gctcctgcac accttttaaa ccggcatcct caaacagttt
      781 agagagcgtg ccaaatttag cgcatcgaaa gggcgtaggt gcatcgggtg ggggcacagg
      841 tatagataac atgctattaa tgacacctct aatcgtcgtc gcccacgggt ttttggcagc
      901 gtctgcccag gccgagagag caattttatg gtcaggctta agcaccctaa ccatttctcg
      961 gattccttcg ctcatactgg gaaagtacat gataccgaaa cggcacataa ccgcaccgaa
     1021 agagttatcg gggaatggct gtcgttcgtc atttaaggcg atagcctcat aatttttgat
     1081 gacacgctcg tgcgcatgca ggttggccag cttgatcatg tcctctgcaa tatctgtacc
     1141 gacgactttc tcttttggaa tatgactggc cgcagttagt cctggttctc ctgtgcccgt
     1201 tgagacgtcc aggacttgat atccgtctgt gagatcggca acttcaatta gtttttcacc
     1261 gataggtcgg aagaatgcca taaagaactc atcccacttt ttccagccag acgaaaattt
     1321 attccaagct tgacgttgtt gttcccgaag ttcgtgagta tcaatcatgt tgttagttta
     1381 gctcatttca aacgcaaagt aactcgttta actctttttc agttggctac acgaactgtc
     1441 tttccttaag accgcgtcaa gttcaatcct gacatacaag atataccacc tttctctcat
     1501 gcaaaacgat gttcatttta caaagttcga accttttttg aaacaaactc tgacgaggaa
     1561 tgaatcgccc cgcccttgcc acgccgcgag gcatcttctt tccacttgcg aaatcctgaa
     1621 ttgcggaagg ggagggattc gaaccctcgg gcggatttaa agccgccacg gcttagcaag
     1681 ccgctcgttt aaccgctcac gcacccttcc atttactgca tgctctatct ttacataaaa
     1741 aagtgctctc gcaaaatatg cttacgctgt ttatactttg gataactata ctcatgtctc
     1801 aaacctataa aatacgcaca aaagtgtggc tctatcccgg tatgaattcg acaggctcac
     1861 cacagagtgg ccggtggcat tttgcgagca ttgataaaaa aatatcggtg aaaataaagg
     1921 aagggtgggg gaatagaaaa cgtcgcggct ggggttcggt tccggtgaag gtaacactcg
     1981 gtaaaagctc ttggaaaact tccattttcc ctgacaaaaa ctcaacctat ctcttgccga
     2041 tcaaaaaaga ggtgcgcaaa aaagaagcta tcttcgacgg cgattcggta actttcagaa
     2101 ttgaaattaa gtagccggtg ttttgtttgt ttcattattc tctgcacttc ataacggaat
     2161 tttgcaaaat actagaatac ggtttaagat tatgggattg aatcggtgcc gttcttcaag
     2221 accgaagaag tccagacgat cggaaggtag aaacggatgg tagaaattaa aagttcacgc
     2281 acagctttgg gagaccttaa aataaataaa aatgtcagtt aattttagaa aaactgagcg
     2341 gattattaag ggggtagcga accatcggcg gcttaaaatt ttagatctgt tgagaaggga
     2401 accggaatta tctgtcgacg agattgccga caaaacgaaa tgcggataca tgaatgcatc
     2461 tgatcatatc cgcaagctcg ctctggccgg tcttgtaatg aaacgaagcg acagcacatc
     2521 tgttcgacat aaactgacgg cacttggcga gcatattcta gtattttgca aaaaactaga
     2581 ataggaggtt tactctcatg gccgcgttta aaatttaaca agctttaaac tatggaagaa
     2641 cttgaactga catacctcgc aaaatatttg cctaaggatt tggagaagtt tctgtcaaaa
     2701 gaaatactcg atatttatat tcccgagtcg tcaaaccatc caacaattcg tattcgtaaa
     2761 tctggcgcaa aatacgaaat aacgaaaaag gagcctatca atgcgcatga tctgtctcgt
     2821 caacttgagc aaacgattcc attaacggag gctgaataca aagactttga aaaactttgc
     2881 ggaaagcgtc tctcaaaacg ccgctatttt tacaagactc acaatacagc tcatgagatc
     2941 gatgtattta cgggtgatct tgcaggtctt gtggtggtag acgttgaatt tagtacggaa
     3001 gcagaaaagg cgagctttgc aatgccggat ttctgtttag tggatgtaac gcaagagaaa
     3061 ttcatcgccg gcggcatgct ttgcggcaaa acatacaaag acatagaacc gaatctcgaa
     3121 aaatttggtt acaaaaaaat ttgaaaatag gtttgacttt accctgtctc tctatagtat
     3181 ggtgaattag cgaaatgctc ttcgagaaat actgtttctg aggttaactt ctcccacgac
     3241 cgaaacgaac actgtatatg accaccatcc atgaacttcc attccgaaaa tcaaaaatcc
     3301 ggagcaccgt cgaagccaca ctcgaagcca cgggcggact cttgcgcctt gcgcccacct
     3361 gggtgccgcg ctcatttctt cagcccggac ttcgtcttaa gctccatcca aaggacacat
     3421 acgcctacgg actgaatcgc ggcggcattg acgaacgctg gtttgcgtca acgactccgg
     3481 cggcaaatga aaatcgcacg cgacacgaag gcctctcgtt tgtcgtcgcc gaccgcgagt
     3541 gcttcatgct cgctcaagca gtcgaagagt gcggcgcgat gctcatcggt aaaaagattt
     3601 ggggcaaata caaaaaatgg cccgtgtact cgaagttctt cgacaacatg ggaccgattc
     3661 cgcaccacat gcatcagtcg aggaaacagg ccaatcttgt cggccaggag ggaaagcccg
     3721 aaagctatta ctttccgccg cagcacaaca acgtcggcaa caactttccc cacaccttca
     3781 tgggcctcga gcccggcaca acacgtgaac aagtgcggcg ttgtctcgaa aactggaaca
     3841 gcggtgacaa cggcatactc gatctctcaa aggcgtatcg cctcaaggtt ggttccggat
     3901 ggctgatcga cccgtgtatc ctacacgcgc ctggttcgct ctgcacttac gagccgcaat
     3961 ggggcagcga cgtctttggc atgtatcaaa atcttgttga agggcgcgaa gtgccatgga
     4021 gcctgcttgt gaaagacatg cccaagagca agcatcaaga cttcgacttc atcgtggaac
     4081 agctcgactg ggacaagaac gtggacccga agttcaagga gcatcattac ctcgagcctg
     4141 ttcccgtcag tggcagcacc aacgaagatc acaccgactt gtggattgtg tacggtacga
     4201 tagatggcga gcagcttttc actgcaaaag agctcactgt cgagccgggc cgaactgtta
     4261 cggtgtggga ccgtggtgcg tacggtctca tttgtgttca ag
//
LOCUS       DATDSU010000017         4497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2022803, whole genome shotgun sequence.
ACCESSION   DATDSU010000017 DATDSU010000000
VERSION     DATDSU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022803"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..3233)
                     /locus_tag="VI981_00515"
     CDS             complement(240..3233)
                     /locus_tag="VI981_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478830.1"
                     /translation="MSNFPRGSEWRKWDLHTHTPLTKLNDKYLSAKGGDVWMDFCERV
                     ERSDVEVFGITDYFSVENYSLFKKKFQSKYPKSEKIFFPNIELRVDRSSNNHNEGYDL
                     HLIFDNELEDAKLEEFLRNLKLDNTDGNNKKLKASELKTENDFKTAFTTIPLIDEALK
                     DTFGDKKPYFKILMSHGHGGVQPEKNNSRKHAVAEESDKRVADIYFGCDDKDREYYLN
                     DRGNVKKKKPCVSGSDAHSFQDFDDKVGKKFLTGGTRYALPTWIKADKTFEGLKQTIH
                     EPRERVFFGEKPEKLLDVESNRSRFADSIKVRHTDSKETSGWFNDEIPLNSGLVAVIG
                     RKGQGKSALTDIIGLCGRTKIDPKDYSFLHKDKFRKKGLAKDYEATLTWLDGTPIPAS
                     LDSEVNATEVEKIKYLPQKYVETICNEDGVSSQFQKEIDKVIFSYIPEENRLNTTSLS
                     DLISVKTSTIDGKVVQIKSDLHSANESIVKLEGKRRPQYLESLKNKLEDKKQELKSLV
                     KPRAILAPKNKLKKSDEKKLTKLTNEIEAVSTQIETAKAASKEVNDKIHKLEKLKAAV
                     GRIAESAESITTNFSEDARLLSIDLSKLLSVKVDNKILDKTARQLSTRKENLEEMLDQ
                     SDTASPKSLYAKKSGLEKQKEEMINALGEDQKKYSAYQGKLKDYIARRKEIEGKPNDL
                     TLETIKSIEAEIKYLDKKIHADLKVALAGRASLVDKLYSELKQKADFYKEIYRPLQRF
                     TEAEKKTQEKSGSVLGFDAGIVFNKGSFPTRFLGYINQNRDGSFQYTDKGQKVLTKIT
                     EKADFSESAGVKIFTQELIDHLEYDKTKNPEKENIVYPQLKGGDQENIEFYDYVFGLE
                     YLDVKYKVLFNGKDLNSNEFSLGEKGALLLIFYLLIDKDRMPLIMDQPEENLDNESIF
                     ELLVPYIRRAKLKRQIIIVTHNPNLAIVCDAEQIVAASMKKQKNQIRYTFGSIENREM
                     NQKASDVLEGTLPAFDIRDEKYIRR"
     gene            complement(3271..3480)
                     /locus_tag="VI981_00520"
     CDS             complement(3271..3480)
                     /locus_tag="VI981_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4478831.1"
                     /translation="MMKSEQIKLGKNLKRIRTQKNVSQGDIARALKVGRSFITNIENG
                     KTNPTLATIARIAKAIGVSVGELMK"
     gene            3541..>4497
                     /locus_tag="VI981_00525"
     CDS             3541..>4497
                     /locus_tag="VI981_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY4478832.1"
                     /translation="MIYFLYARKSTDVEDKQILSIEAQLAELREIAKRDNLHIAEEFI
                     EKRSAKMPGRPVFNEMLKRIQQGEAQGIICWKIDRLARNPVDGGQIQWLLQEGVIRHI
                     QTHDRSHYPNDNVLMMSVELGMATEYIRQLSANTSRGLKHKAKMGIYPGLAPFGYLNN
                     PLTKTVAVHRKNAKLVRKTFEMYTTGKVRLENLAEFWEKSGVLSRGSRRIHISRLSFI
                     LQNPFYYGHFRYCGEIHEGKHEPIITKDLYDRANVILRGRGHRQDIKNDPSPYCGLLR
                     CGGCGMGITAEVRVKYQKNGNIHHYTYYRCTRKSRMHKCVEAP"
BASE COUNT         1077 a         1066 c          943 g         1411 t
ORIGIN      
        1 cgattttgcc gattttttcg cgggcggagc gaagctccgc ccaatgattc gccgcaattc
       61 cgcacgcttc gcgtgtcgtg aggttcaaaa atacaaatcc gaagattttt ggagggagga
      121 ttttttggag gggaggtcga atacagaata ccaacacttg ccccaccctc ccgtgcgcgt
      181 gttgaaagcc ttttgccgac ttgttagatc ggcaaactcc cacataagaa aactaaacct
      241 tatcgcctaa tatatttttc gtctctaata tcgaacgcgg gcaatgtgcc ttccaaaaca
      301 tcagacgcct tctggttcat ttcgcggttc tcaatactgc cgaacgtgta tctaatttgg
      361 tttttctgct ttttcattga agcggctaca atttgctccg cgtcgcagac aatcgctaag
      421 tttggattgt gggtaacgat gataatctgc ctttttagct ttgctctcct gatataggga
      481 acgagtagct caaagattga ttcgttatca aggttttctt cgggctgatc cataatcaag
      541 ggcattcggt ctttatctat gagtagatag aaaatgagca gaagtgcccc tttttcgccg
      601 agcgaaaatt cattgctgtt caagtccttt ccgttaaaca aaactttata tttaacgtct
      661 aggtattcca gcccaaagac gtaatcgtag aactcaatat tctcttgatc tccacctttt
      721 aactgcggat atacgatgtt ttctttttcg ggattttttg tcttgtcata ctcaaggtgg
      781 tcaattaact cttgggtgaa tatcttaact ccggctgatt cggaaaagtc agccttctcc
      841 gtaatcttgg taagcacctt ttgtccttta tcggtatatt gaaaactacc atcgcgattt
      901 tgatttatat aacctagaaa ccgagtagga aaagaacctt tattgaaaac gatgcccgca
      961 tcaaatccaa gtacgctacc tgatttttcc tgcgttttct tttccgcctc tgtaaatctt
     1021 tggaggggtc tgtatatttc tttatagaaa tcggcctttt gttttagctc cgagtagagc
     1081 ttatcaacta gagaagctct accggcaagt gccaccttga gatcggcatg gattttttta
     1141 tcaagatatt ttatttccgc ttcaattgac ttaatagtct caagcgttaa atcgttaggc
     1201 ttgccttcga tctcttttct tcgtgcgata tagtccttta actttccttg gtaggcagaa
     1261 tactttttct ggtcttcgcc taaagcgtta atcatttctt ctttctgctt ctcaagcccc
     1321 gactttttcg catacagact cttcggcgaa gcggtatcgc tttggtctaa catttcctcc
     1381 agattttctt ttctggtaga tagctggcgt gccgtcttgt ctaggatttt attatcaact
     1441 ttaacgctta atagttttga tagatcaatc gagagaagtc ttgcatcctc cgagaagttc
     1501 gtcgtgatac tttcagcgct ttccgcgata cggccaaccg ccgccttaag tttttctaac
     1561 ttgtggattt tgtcattaac ttccttggag gcagccttcg cggtttcaat ctgtgttgaa
     1621 acagcttcga tttcgtttgt cagttttgtg agctttttct cgtcgctttt tttgagtttg
     1681 ttcttcggtg caagaattgc tcgcggtttt accaaactct ttagctcctg ctttttatcc
     1741 tcaagtttat ttttcaggga ttccaaatac tgcgggcgac gtttgccctc caatttgaca
     1801 atgctttcat tcgccgaatg aaggtcgctt ttgatctgaa caaccttacc gtcaattgtg
     1861 gaggtcttga cgcttatcaa atcggaaaga ctggtggtgt ttaatctgtt ctcctctggt
     1921 atgtaggaaa agatgacttt atcaatttct ttttggaatt gagaacttac gccgtcctca
     1981 ttacagattg tctcgacgta tttttggggt agatatttta ttttctcaac ctcggttgcg
     2041 tttacctccg aatccaaact cgcagggatt ggcgttccgt ctaaccatgt cagggtggct
     2101 tcgtagtctt tcgccaatcc ttttttgcga aacttatcct tgtgtaagaa cgaataatct
     2161 ttcgggtcaa ttttagttct tccgcaaagt ccgatgatgt cggtcaaggc actcttgccc
     2221 tgccctttcc ttccgataac agcgaccaag ccggagttca agggaatctc gtcgttgaac
     2281 cagccagaag tttctttgct atccgtatgc ctaaccttta tcgaatctgc aaacctcgaa
     2341 cgattcgatt ctacgtctag gagtttttcg ggtttctccc cgaagaaaac tctttctcgc
     2401 ggctcgtgga tagtctgctt cagaccttca aaggtcttat ccgcctttat ccaagtgggt
     2461 aaagcatacc tcgtgccgcc agttagaaac ttctttccaa ccttatcatc aaagtcttga
     2521 aaggagtggg cgtcgctccc agatacacac ggctttttct tttttacgtt gccccgatca
     2581 ttcagatagt attctctgtc cttatcgtcg caaccgaaat aaatatccgc aactctctta
     2641 tcactttcct cggcaaccgc atgtttccga gagttatttt tctctggctg gacaccaccg
     2701 tgtccatgag acattaagat tttgaaatac ggttttttat ctccgaaggt atctttaaga
     2761 gcttcatcaa taagtggaat tgttgtgaag gcagtcttaa agtcgttttc agttttgagt
     2821 tcgctcgctt tgagtttttt attgttgccg tccgtgttgt ccagtttcag attcctcaaa
     2881 aactcttcga gcttcgcgtc ttcaagctcg ttgtcgaaaa ttaagtgcag gtcgtagccc
     2941 tcgttgtgat tattcgacga gcgatctact cgtaactcta tgttaggaaa aaatatcttt
     3001 tccgattttg gatatttgct ttggaacttc tttttgaaca gtgaataatt ctcaacggaa
     3061 aagtagtccg ttatgccgaa gacttctaca tctgaacgct cgacccgttc acagaaatcc
     3121 atccagacat cgccgccttt tgcgcttaag tatttatcat ttaacttcgt caaaggagtg
     3181 tgggtgtgta agtcccattt tctccattct gaacctctag ggaaattact cataaagttt
     3241 atttactcca atcgcgtatg aaacatagtt ttatttcatt aattccccaa ctgatacccc
     3301 gattgctttt gctattctcg cgattgtcgc aagtgtcgga tttgtcttgc cgttttcaat
     3361 gttggtaata aagctcctac ccactttaag agcgcgggca atatcaccct gcgatacgtt
     3421 cttttgcgtc cgtattcgct ttaagttctt accgagcttt atttgttcac tcttcatcat
     3481 tcgcagtata ttctccggta tattgcgata caatggtata tcataatata ctactaatat
     3541 atgatttatt tcctttacgc ccgcaaaagc accgatgtcg aggataagca gattctctcc
     3601 atcgaggcac aacttgccga attgcgggag attgccaaac gcgacaatct ccatatcgcg
     3661 gaggagttta tcgaaaaacg ctccgcgaaa atgcccggac gccctgtgtt caacgaaatg
     3721 ttgaagcgga ttcaacaggg cgaagcgcag ggcatcattt gttggaagat tgatcgcctc
     3781 gcgcgcaatc cggtcgatgg cggacaaatc caatggctgc ttcaagaagg agttatccgc
     3841 cacattcaga cgcacgacag aagccactac cccaacgaca acgtactgat gatgtccgtc
     3901 gagcttggta tggcgacgga gtacatccgg cagttgagcg ccaacacttc acgcggactg
     3961 aaacacaagg cgaagatggg aatctatccc ggcctcgctc cattcggata cctgaataat
     4021 ccgctcacca agaccgttgc agttcaccgc aagaacgcga agctggtcag aaaaacattc
     4081 gagatgtaca cgacgggaaa agtccgtctg gaaaatctcg ccgagttttg ggaaaagtcg
     4141 ggcgtgcttt cccgaggcag tcgccgcatc catatctccc gattgtcttt cattctgcaa
     4201 aatcctttct actacggaca ctttcgctat tgcggagaga tacacgaggg caagcacgag
     4261 ccgatcatca caaaagattt atacgacaga gccaacgtga ttttgcgagg ccgaggccat
     4321 cggcaagaca tcaagaacga cccaagtccc tactgcggcc ttttgcggtg cggcgggtgc
     4381 ggtatgggca ttacggcaga agtgcgggtg aagtaccaaa agaacggcaa catccaccac
     4441 tacacgtact atcgttgcac ccgcaaatcg cggatgcaca agtgcgttga ggcacct
//
LOCUS       DATDSU010000018         3652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_685519, whole genome shotgun sequence.
ACCESSION   DATDSU010000018 DATDSU010000000
VERSION     DATDSU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685519"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VI981_00530"
     CDS             <1..770
                     /locus_tag="VI981_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478833.1"
                     /translation="LSTSAQTISGGFPAQSIWLSKTNAVEGDRIEIFTVLHNGSEVEI
                     RGTLVFSVDGAKIATKEFELESGNNKFESTLWSSKAGKHKISASVAEAFDNSGKQIIL
                     SNQNTGEIEINIANPPPPTAIEKTVSVVGGVATNVASTAFPIISSVTKNIYEATESFR
                     MGGFNFAKEMIADSSGGIKNEPLVLGATTSKSNIPSTNTGIADENEPSFFSKLAQLAS
                     PAILFSFGSRAIFYPLLIILALALLYMLGRIVNRPKY"
     gene            complement(807..1238)
                     /locus_tag="VI981_00535"
     CDS             complement(807..1238)
                     /locus_tag="VI981_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478834.1"
                     /translation="MDHQKRLQFLGIIALICFFFALVVLWPERFGLCNEADRTCIYGY
                     LNYESTSVSVLLFSLSILLCSIVLYFLRQGVYRAWTKFMLWWIPFSVILIALTPSYGG
                     SGLDGGVPGLDREMIIWLAASAFFIISLAIIAWKSFQLRRK"
     gene            complement(1243..>3652)
                     /locus_tag="VI981_00540"
     CDS             complement(1243..>3652)
                     /locus_tag="VI981_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478835.1"
                     /translation="AGAPGGPEQITIQDEGTYTPPLNVEAPAYITAPDTGPNGEFLFD
                     VFANLYRVTGPGEREFISTLYQHTEYDDPDDPDDGDIKFAWPAAGAYELDILSFPPPQ
                     VSFEDRLRRWVANILFTNIAHAQFFEPTLIETLHFTIEDVSASCVADCFSNVLFLPGL
                     ESSRLYRPNVIGPDIHLWEPSFFGLDHEKLMMKPDGTPALPGIYTKDIIDEAFTANIY
                     KSFIEKMDELVADGTINEWNDFPYDWRFDINDIVNYPTALATTTISLIAEVERLAAGS
                     KSGKVTIISHSNGGLVGKVLLSELEYQGKANLVGKFIMVAVPQLGTPKTIATLLHGEA
                     MPSQLFPFVAKAREIREMAENMPSGYALLPSAEYFARVLDPVVEFVDNGSPLMQPFVD
                     SYGLAITNTTELNDFLLGTDGRSKPAENDVMNPNILNADLITQGEANHIVLDSWHAPD
                     GIEVIQIVGWGVPTIRGIRYKEARLTGSACAVSNCRFLDHEPILTIDGDETVVVPSAS
                     LLTINTYYLNLREYNRFINLKINREHADIMEMDQLLLFVANLIQDDSTIPLFVETSKP
                     TFDPDSAPALRISIHSPVSLDLYDESGRHTGVATSTIGLPVIEEQIPNSYYFDMGEGK
                     YAGADVLGTTTIRLHGLALGTFTLDIERTVGDMVTASTTFANIPVTASSTALLDIGDS
                     TNLPASKLSVDVDGDGVEDADISAGDGLTIPELLGLWRGLVNSLDLPIKKKQKLIKMI
                     DKLEKQILKDQKDKKFKKYLMGYVLKDIEAKIKLFEKRKFLTKNEVSELLAILANVGS
                     LVLH"
BASE COUNT         1023 a          865 c          784 g          980 t
ORIGIN      
        1 tcctttcgac ctcagcacag acgatttctg gaggttttcc ggcccaatca atctggctat
       61 cgaaaacaaa tgcggtcgag ggcgatcgaa tagaaatatt cactgtcctt cacaacggga
      121 gtgaagttga aatccgcgga acgctggtat tctccgttga tggcgcaaaa attgcgacaa
      181 aagaattcga gcttgaatcc ggaaataata aattcgaatc cactctgtgg tcctcaaaag
      241 ctggcaagca caaaatctcg gcgtcagttg ccgaggcttt cgataatagc ggtaagcaga
      301 taattctttc gaatcagaac acaggagaaa ttgaaataaa tatcgcgaac cctcctccgc
      361 ctactgcaat tgagaaaaca gttagcgttg tcggaggcgt tgccacgaac gtcgcttcaa
      421 ctgctttccc gatcatatcg agcgttacaa aaaatattta tgaggcaacg gagtctttta
      481 gaatgggcgg gtttaacttt gcgaaagaaa tgattgccga ttcttccggc ggtataaaaa
      541 atgagccgct agttttaggc gcaaccactt ctaaatctaa tatcccatca actaacacgg
      601 gcattgcgga cgaaaacgaa ccttcatttt tttcaaaact tgctcagctt gcatcgcctg
      661 caattctttt cagtttcggc agccgcgcaa ttttctatcc ccttcttata attcttgcct
      721 tagcccttct ctacatgctt ggtagaatcg tcaaccgccc aaaatactag tccgaaaacc
      781 ccagagaacc gacctctgtg tttttgctat tttctccgca attgaaaaga cttccaagca
      841 atgatagcga gtgagattat aaaaaatgca cttgccgcaa gccagataat catctcgcga
      901 tcaaggcccg gcacgccccc atccagtccg ctacctccat agctgggcgt aagtgcaata
      961 agaattacgg aaaaaggtat ccaccacaac atgaatttcg tccaggcacg gtagactccc
     1021 tgacgcagaa agtataaaac gattgagcac aagagtatcg atagcgaaaa tagtaaaaca
     1081 gataccgaag tagattcata atttaggtat ccgtatatac aggttctatc agcttcgttg
     1141 cataaaccaa accgctctgg ccacaaaaca actaatgcaa aaaagaaaca tatcaaggca
     1201 ataatgccaa gaaattgaag tcttttttgg tggtccataa ttttagtgta gcaccaaact
     1261 tcccacattc gccaaaatag ctagtaattc ggacacctcg ttctttgtta gaaactttct
     1321 cttctcaaat aatttaattt ttgcctctat atctttgaga acataaccca tcaagtattt
     1381 cttaaacttc ttatctttct gatcctttaa gatttgcttc tcaagcttgt cgatcatctt
     1441 tatcaatttc tgttttttct ttataggcaa gtccagggag tttaccaaac cgcgccacaa
     1501 accaagtaat tccggtatag tcagcccatc gcccgcggag atgtcggcat cctccacacc
     1561 atctccatct acatctaccg ataatttaga agcaggtaga ttagttgagt ctccaatgtc
     1621 gagcaaggct gtcgaacttg ccgttactgg aatgtttgcg aatgtagttg aggctgtgac
     1681 catatctccc acagttcgtt ctatatcgag tgtgaatgtg cctagagcaa ggccgtgtag
     1741 ccgtatagta gttgttccga gtacatctgc tcctgcatat ttaccttcgc ccatatcaaa
     1801 gtaataacta ttcggaattt gttcctcaat cacggggaga ccaatcgttg aagttgcaac
     1861 cccggtgtga cgtccagatt cgtcatagag atcaagcgat acgggcgaat gaatgctaat
     1921 tctcagggca ggagcactat caggatcaaa tgtcggtttg gaagtttcaa caaataatgg
     1981 aattgtcgag tcatcctgaa ttagatttgc tacgaataac agcaactggt ccatttccat
     2041 gatatcagca tgttctctgt tgattttcag atttataaat cgattatatt cccgcaaatt
     2101 taaataataa gtatttatcg tcaataaaga tgcactagga accaccactg tttcatcgcc
     2161 atctatagtg agaattggct catgatcgag aaatcgacaa tttgaaaccg cacatgctga
     2221 acctgtgagc ctagcttctt tgtagcgaat accgcgtatc gtaggaactc cccagccgac
     2281 aatttggatg acttctattc catcgggtgc atgccagctg tcgagcacga tatgatttgc
     2341 ttcaccttga gtgatgaggt cagcgttcaa gatattggga ttcattacgt cgttctccgc
     2401 gggtttagaa cgaccgtcag tgcccaataa aaaatcattc agttcagtcg tattggtaat
     2461 agcaagacca tagctatcaa caaatggctg catcagtggt gagccgttat caacaaattc
     2521 aacgacagga tcaagtaccc gcgcaaagta ttctgcagac ggaagaagag catagccaga
     2581 aggcatattc tcggccattt cgcgaatttc tcgagctttc gcgacaaacg ggaacaattg
     2641 cgagggcatc gcctctccgt gcaataatgt cgcaatagtt tttggagtgc cgagttgtgg
     2701 caccgccacc atgatgaatt tgcccacaag atttgccttg ccttgatatt caagctcgct
     2761 caagagcacc ttgccgacca acccgccgtt tgaatgagag atgatcgtca ctttccccga
     2821 tttcgagccg gcggcaagcc gctccacctc tgcgatgagc gaaatagtgg ttgtcgcaag
     2881 agcagtcggg tagttgacga tatcatttat gtcaaatcgc caatcgtatg gaaagtcgtt
     2941 ccactcattg atagtcccat ctgcaacgag ttcgtccatc ttttcaatga acgatttata
     3001 tatgttcgca gtaaatgcct catcgataat gtccttcgta taaatccctg gtagcgcagg
     3061 agttccgtcg ggcttcatca ttaatttttc gtgatcgaga ccgaaaaatg atggctccca
     3121 taaatgtatg tcagggccga tgacattcgg ccgatatagc ctgctcgatt caaggccggg
     3181 caaaaagagc acgttcgaga aacaatctgc gacacacgaa gcggatacgt cttcgatcgt
     3241 gaagtgcaga gtttcgatca gggtcggttc aaagaactgc gcatgcgcaa tattggtgaa
     3301 tagaatgttt gccacccagc gacgcaatcg atcttcaaaa cttacttgtg gcggtgggaa
     3361 cgaaagtatg tcgagctcat atgctccggc cgcgggccaa gcaaatttta tgtctccatc
     3421 atctggatcg tcggggtcat catattcggt gtgctgatac aatgtagaaa taaattcgcg
     3481 ctcgcctggg ccagtcactc gataaagatt tgcaaaaaca tcgaaaagaa attctccgtt
     3541 gggaccagtg tctggtgcgg tgatatacgc gggtgcttcc acatttagcg gcggcgtgta
     3601 cgtaccttca tcctgaatcg ttatctgctc cggcccgccc ggtgctccgg cc
//
LOCUS       DATDSU010000019         4928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_519642, whole genome shotgun sequence.
ACCESSION   DATDSU010000019 DATDSU010000000
VERSION     DATDSU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519642"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..250)
                     /locus_tag="VI981_00545"
     tRNA            complement(160..250)
                     /locus_tag="VI981_00545"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Ser,seq:gct)
     gene            530..2929
                     /locus_tag="VI981_00550"
     CDS             530..2929
                     /locus_tag="VI981_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY4478836.1"
                     /translation="MSRKISIGNVKVVTEEDVKLKIISPILVALGYSESIENEIEYER
                     RIPIGRGKYVFPDIVVNIVGATAFVLDAKGPEENLDFYKDQVMAYGLLLRATYSVLCN
                     GKELRVFDTKTGKTVWSKEVTEKPDFLSKKELSAKRHTSIDSLTAEQEEQAKKTLLVF
                     EGIKEFSTILYRCESIIRDRDGLTGADAFDELSMILFTKMYEERLAGQENRKSRFGID
                     EHTDARYIKKDLFERGRDANKDIFNGDESIKLKDESIKEITQLIQGYTLQETEVDVKG
                     RAFEIFLGRTFTGKLGQFFTPRTIVDFMVSFVNPQRLSSDKTSYTVIDPSCGSGGFLI
                     RVFMHVAKHIQETEKDNKKKQGLLNVLARDQIYGVDIDPRLVRIAKMNMVLHGDGHGG
                     IYRNSGLEAHRHEKLAAKKFDLVITNPPFGNKDEGVILKQFELGEEYARGTPPAQLRE
                     ILFVEECIKLLKDGGELAILLPDGVLNNSSTDYVRNYIKKHTIIQAVISLPDRAFKAA
                     GANSKTSILFLRKRKSDGDRQGAVFMAVAEEVGYDRSTKEAEPIAQNDLPLILRLYRE
                     FLTTQHHRTIQDGLDGVHIIAEEPACFMIDSDLLEKRFDASYFYAKYVFKLDVPSVKI
                     ADIAYISEDRINPNNSPERVFKYIQFSHVEKRLGNIVSWDELSGEEVPSRGRLLLREG
                     DVIAATVKDSEENVAIVPPALDGEIASTGFLVLRPKEGMTREALYVLLRLKTTFNQIR
                     WMVSGTIQPAIKDEDYIEIVVPKLDLATIKNITKKITEVEKARQQIKYELDSIGEILL
                     K"
     gene            complement(3389..3862)
                     /locus_tag="VI981_00555"
     CDS             complement(3389..3862)
                     /locus_tag="VI981_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478837.1"
                     /translation="MNIQWKWAFIHALFVYACMMIGSVIAVSLLKLGALNPANLIISA
                     ESAFGYLGAVFIVSAITILGGAIGFAVSVFRTELYRGRHIFAVAILVLIAQIAITLFS
                     TDFFNLQTFELSDFIAVFEIIPRISMPIVLSTLLGWVLGNLARKLFKRAKVSPAG"
     gene            3981..>4928
                     /locus_tag="VI981_00560"
     CDS             3981..>4928
                     /locus_tag="VI981_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY4478838.1"
                     /translation="MFTTWHNKELPAVLEALRTTENGLSADEAIRRLKEDGLNALPEA
                     KPDGLAAMFLRQFRSPLIYILLAASGIVFLMGEVIDGSIILAVLLFNAIVGTIQEGKA
                     QNTLLALKKFVETTATVLRDGQELTIPDREVVRGDVLVLQEGEKIPADARVIATNNLK
                     IDEASLTGESEPVSKNPEAIQKDALAVPEQRNMVFKGTNIVAGNGRAVVVATGQNTAI
                     GKIAVQISAIDSEIPLKANIRHLSRAIIIVVAVISAFLFVLGLIRGQDVVTIFTTVVS
                     LAVSIIPEGLPIVITLVLATGVWRMSKRNALIKKLQAVEA"
BASE COUNT         1433 a         1029 c         1191 g         1275 t
ORIGIN      
        1 gggaacaacc ggacaaaaaa tcagagaagc gccgcggttc gctgagagaa tcgttgcgcg
       61 cgaaaggaga gattttgcag gaactcaaag accgcgcacc ggagacggaa ctacaaatca
      121 gagactaaaa aaaccgccct atggcggttt ttttatttgc ggagagggag ggattcgaac
      181 cctcgataca ggtttgatcc tgtatagcgg attagcaatc caccgctttc agccgctcag
      241 ccacctctcc tgtctgctgt atcttacgtg agttgcggta tcttatcaaa tgctttatgt
      301 gatatgaatt cgaacgctgg agcagaccca ctgccggcaa gcgggggaat gaggtggtgc
      361 ctagaccaat cctgcgcgat ggcgtaggat tgggtcggag gagaaatccc ttcctctccg
      421 cagattacca agtttgaacc aaataaatct gagctaggca acctgttgat aaccaggctg
      481 ttgccggaat cgtatttgtg ctaattttat agcaaggatt caattttata tgtcacgaaa
      541 aatctcgata ggtaacgtca aagttgttac agaagaagat gttaagttga aaattatttc
      601 tcctatactt gttgcacttg gatatagcga gagcattgaa aatgaaatag agtatgaaag
      661 gcgaatccct attgggcgag ggaaatatgt atttcccgat attgttgtaa atattgttgg
      721 cgcaacggct tttgtattgg atgcaaaagg tccagaagaa aatctagatt tctataagga
      781 tcaagtgatg gcgtatgggc tacttttgcg cgcaacgtac tctgttctat gcaacggcaa
      841 agaactacgg gtttttgata caaaaacagg aaaaactgtt tggtcaaaag aggttactga
      901 aaaaccagac tttctatcaa agaaagaatt gtcggccaaa cgacacactt caatcgattc
      961 tttgacagcc gagcaggaag aacaggcaaa gaaaacactt ttggtgtttg aaggtattaa
     1021 ggaattttct accatcctct atagatgcga atcaataatt cgagatcggg acggactcac
     1081 tggggctgac gcctttgatg aattgagtat gattcttttt acaaaaatgt acgaggagag
     1141 attggcgggt caagaaaatc gcaagagtcg ttttggtatc gatgaacata cggacgcacg
     1201 atatataaaa aaagatttat ttgagcgggg acgagatgca aataaagata tttttaatgg
     1261 tgatgaatct ataaaattga aagatgaaag tattaaggaa atcactcagt taattcaggg
     1321 ctatacactt caggaaacag aagttgatgt aaagggacgc gcatttgaaa tcttcttagg
     1381 aagaacgttt acagggaaac tagggcaatt ctttactcct cgcaccatcg ttgattttat
     1441 ggttagtttc gttaatccgc aacgactttc ttccgataaa acctcatata ctgtcattga
     1501 cccgtcttgc ggaagcggag gcttcttaat cagggtgttt atgcacgttg ctaaacacat
     1561 tcaagagaca gaaaaagata acaagaaaaa acaagggcta cttaatgttc tcgcgcgtga
     1621 tcagatatat ggtgttgata ttgatccacg ccttgtccgt atagccaaaa tgaatatggt
     1681 tctccatgga gatggacatg ggggcatcta ccgtaacagt ggacttgagg cccataggca
     1741 cgaaaagtta gcggcgaaga aatttgactt agtaattacc aatcctccat tcggcaacaa
     1801 agatgagggg gtaattttaa agcagttcga attgggtgag gaatacgcaa gaggtactcc
     1861 tccagcacag ctgcgagaaa ttttgtttgt agaagaatgt ataaaactct taaaagacgg
     1921 gggcgaatta gctatcttac tccctgacgg ggttctcaat aattcctcga cagactacgt
     1981 aaggaactat attaaaaaac acacgataat tcaggctgtc ataagccttc cggaccgggc
     2041 atttaaagct gcgggcgcaa atagtaaaac gagcattctg tttctgcgta aaaggaaatc
     2101 tgacggggat aggcagggtg cagtatttat ggccgttgct gaagaggtgg gctatgaccg
     2161 ttctacaaaa gaggcagagc cgattgctca aaatgatctc cctcttatct tgcgtttgta
     2221 tcgagaattc ttaacgacgc aacaccaccg caccatccaa gatggtttgg atggcgttca
     2281 tataatcgcg gaggagcctg catgctttat gatcgattct gatctgcttg agaaacgctt
     2341 cgacgcaagt tatttttatg ctaaatatgt tttcaagctt gatgttccaa gcgtaaagat
     2401 agcggatatt gcttatattt cagaagatag aattaatcca aataactctc cggagcgtgt
     2461 ctttaagtac atccaattca gtcatgttga gaagcgactc ggaaatattg tttcttggga
     2521 cgaactttcg ggagaggagg tgccaagtcg cgggagactt ctattgcgtg aaggggatgt
     2581 cattgctgct acagtaaaag atagtgagga aaatgttgca atagtacccc ctgcgcttga
     2641 tggagaaatc gcctcaactg gcttcttggt tcttcgtccg aaggagggca tgacacgcga
     2701 agctttatac gttctccttc gactaaaaac aacatttaat cagattcgct ggatggtatc
     2761 tggcacgatt cagccagcaa tcaaagacga agattatata gagattgtag tcccgaagtt
     2821 ggacctagct acaattaaaa acataactaa gaagattact gaggttgaaa aagctcgaca
     2881 gcaaattaaa tatgaactcg attcaatcgg agaaattctt ctaaagtaac tttagggcgt
     2941 aattgaataa tcaacggcat ttataccttt atctgattta ggcctcggta cctcaaacca
     3001 ttgaggatgt tgcttgaaca tggctcgtaa ttcacttatg cttttttcaa gatccttgaa
     3061 tttgagccac gtttttggtt caaagttccg gcaatcgtct ccgccaaggt gttcgtgtta
     3121 gaattatagt gtttagtgga atttaattaa aaaagtcgct acatgaatct gaaatttaca
     3181 aacttagcgg tatttataat ttttttcgga attgctttaa ttgaggcgct tcaaaaacaa
     3241 aattggttgg aggcggcgct ctttttggct ttgggcgtat tgtcgctttg ggcggatatt
     3301 aaaaaaggtt agatttttaa tcgccggcaa ataattgtca gcgggggtct cgtcaccacc
     3361 gcggagagta gttcgcgctc agtagtcttt atcccgcagg gcttactttc gctcgcttaa
     3421 ataatttgcg cgcaagattc cccaataccc agccaagtag cgtactgagc acgataggca
     3481 tagatatacg tgggatgatt tcaaatactg caataaaatc tgatagctca aacgtttgca
     3541 gattgaaaaa atcagttgag aaaagagtta tggcaatctg tgctattaac acaagtatag
     3601 caactgcgaa aatgtgtctg ccacggtata gctctgttct gaacacacta actgcaaaac
     3661 caatagcgcc acccaaaata gttattgcag acactataaa gacggcgcct agatatccga
     3721 acgcagactc ggcactaatt atgagattag caggattgag tgctcccaat ttgagaaggc
     3781 taactgcgat aacggacccg atcatcatgc atgcgtagac aaaaagagcg tggataaatg
     3841 cccatttcca ttggatgttc atatgttcat tctaacaaaa tactcgtctt attcaacttt
     3901 tatatatgca caaaaattga ggctacccac ctcgttattt ttcgaaatgg tatagtggtt
     3961 ttgtgtagga aacactttac atgtttacca cctggcacaa taaagagctg ccggcggtcc
     4021 ttgaggcact tcgtaccacc gagaacggac tgagtgccga cgaggccatc cgacggctca
     4081 aagaagatgg actcaacgct ctccccgaag cgaagcccga cggccttgcg gcaatgttcc
     4141 tgcgccagtt ccgtagcccg ctcatctaca tattgcttgc cgcaagcggc atcgtattcc
     4201 ttatgggcga ggtcatcgac ggctcgatta tccttgccgt gctcctgttc aacgcgattg
     4261 taggcactat acaggaaggc aaggcgcaga atacgctctt ggcgctcaag aagttcgtgg
     4321 agactactgc caccgtgctg cgcgacgggc aggagctgac gataccggac cgcgaggtag
     4381 tgcgcggcga tgtgctggtc ttgcaggagg gcgagaagat ccccgccgac gcccgggtca
     4441 tagccacaaa caatctcaaa atcgacgaag cgtcccttac cggagagtca gagccggtga
     4501 gcaaaaaccc cgaagcaata caaaaagatg cgcttgccgt acccgagcag cgcaacatgg
     4561 tgtttaaggg cacaaatatc gtcgcaggaa acggcagagc cgtggttgtt gcaaccggac
     4621 agaatacggc catcggcaaa atcgcggtgc agatatcggc tatagactcg gagattccgc
     4681 tcaaggcgaa catccgccat ctctcccgcg ccatcatcat tgttgtggcg gtcatcagcg
     4741 cctttttgtt cgtactcggc ctcatcagag gccaggacgt tgttactatt tttaccacgg
     4801 tcgtatctct ggcggtgtcc atcatccccg agggtctgcc catcgtcatc acgctggtgt
     4861 tggcgaccgg cgtgtggcgc atgtccaagc gcaacgcgct gataaaaaaa ctgcaggcgg
     4921 tggaggca
//
LOCUS       DATDSU010000020         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2359342, whole genome shotgun sequence.
ACCESSION   DATDSU010000020 DATDSU010000000
VERSION     DATDSU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359342"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VI981_00565"
     CDS             complement(<1..313)
                     /locus_tag="VI981_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478839.1"
                     /translation="MKEIGLGVAGLGLLYLTRRKMTAELSRLVVRLGGGQREFVWLWS
                     AIFLPGTIIHEVSHFLAAALTGARTGRVEILPELPRKTLEGKIKTQTSHRLGFMETQR
                     LG"
     gene            446..519
                     /locus_tag="VI981_00570"
     tRNA            446..519
                     /locus_tag="VI981_00570"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:478..480,aa:Trp,seq:cca)
     gene            560..880
                     /locus_tag="VI981_00575"
     CDS             560..880
                     /locus_tag="VI981_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4478840.1"
                     /translation="MNGAYLHGVPLYYTYVLQSLRDTKLYIGSTSDLKKRFQLHQVGQ
                     VFATKGRLPVKLIFYEAFQMKDDAIRREKYFKTNPGKRSLRLMLKAYFSAGIRPALSA
                     AKSV"
     gene            891..1085
                     /locus_tag="VI981_00580"
     CDS             891..1085
                     /locus_tag="VI981_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478841.1"
                     /translation="MSRTIRRNIESELVGNPTLIIPEGMVPDHEKFIVNAFQALDADQ
                     GKISPTDHGHAAGLSLLSCL"
     gene            1316..1918
                     /locus_tag="VI981_00585"
     CDS             1316..1918
                     /locus_tag="VI981_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY4478842.1"
                     /translation="MAHCVLFIDGENFLHKIKEVLKKEGVKKNVADLAQIDLHKLLQE
                     PLKGFAVSRKIFYVSRLHLHPETKKKSNELIKFQRIIGNNLTRQGFEFVITGNVRAQK
                     IGNKVVFREKGVDVKIAVDLVSFSSRKILDCAILCSSDSDLQPAVAELRERKIEIIYL
                     GFEENPNKGLSYTTNRTILLRNSEILAASASLPALSIPAS"
BASE COUNT          568 a          486 c          413 g          541 t
ORIGIN      
        1 gccccaacct ttgtgtctcc ataaatccca gccgatggct cgtctgggtt tttatttttc
       61 cttccagcgt tttccgcggc aactccggta aaatctccac ccgtcccgtc cgcgccccgg
      121 ttaacgccgc cgccaggaaa tggctgactt catggataat cgttcccggc agaaaaatcg
      181 cgctccacag ccacacaaat tctctttgtc cgccccccag tctcacgact aaccgtgaca
      241 actccgccgt catcttccgc cgcgtcagat aaagcagccc cagccccgcc acccccaatc
      301 cgatttcttt cacttctaaa gaataaaaca aaggtctaac ctttgcaatc cgcaaaggtt
      361 agacctttgt gaattttccg gaagaatttt tgccgggaag ctgtataatt cctcttgatt
      421 gacaatcgct ttagttttat taattagggt cgtcgttcaa cggcaggaca gcggtctcca
      481 aaaccgccaa tgaaggttcg attccttccg accctgccat gaaggatgcg cgacttcact
      541 tcgtggaacg atccacgaag tgaacggcgc ttaccttcat ggcgtgccct tgtattacac
      601 ttacgttctc caatctcttc gggataccaa gctttatatt ggttctactt ctgacctgaa
      661 aaaacgtttt cagcttcatc aagttggtca agtatttgcc accaaaggcc gacttccggt
      721 aaagctaatt ttttacgagg catttcaaat gaaagatgac gccatcaggc gagaaaaata
      781 cttcaaaacc aaccccggaa aaagatcgct tagattaatg ttaaaagcgt acttttctgc
      841 cggcatccgc cccgccctct ccgccgccaa aagcgtataa tagccaagtt atgtcaagaa
      901 ctattcgtcg caacattgag agtgaattgg ttggtaatcc tactctgatt attccagaag
      961 gaatggttcc ggatcacgag aagtttatag taaatgcttt tcaggcgttg gacgcggacc
     1021 aaggaaaaat ctcaccgaca gatcatgggc acgctgccgg attaagtctc cttagctgtt
     1081 tgtagtcgat acgggtcgta gttttcattt atcctccttt ctacctgatc tcgatcaagg
     1141 tggagtaatt ctagaacccg agtgatgaaa aggaagtagc aggagtgtat agcatgtcct
     1201 ggacaccgca caaacagtga ttgacttcta aggagaaacg ggttatagta acatcattaa
     1261 cgcctcagcc agcgtcgcaa gacccgctga ggtttttttg atgtacaata tgtttatggc
     1321 tcattgtgtg ttattcattg acggcgagaa cttccttcac aaaattaaag aagttttgaa
     1381 aaaagaagga gtaaagaaaa acgttgcaga cctagcccag atcgatcttc acaagctctt
     1441 acaagaaccc cttaagggat ttgccgttag ccgaaaaata ttttacgttt cccgcttgca
     1501 tctacatcct gagaccaaga aaaaatcaaa tgaattaatt aaatttcaaa gaattatcgg
     1561 gaataatctt actcgtcagg gattcgaatt tgtgataacc ggtaatgtcc gggcccagaa
     1621 aataggaaat aaagttgtct ttagggaaaa gggagttgat gtcaaaattg ccgttgattt
     1681 agtttcgttt tcttccagaa aaattctgga ctgcgccatt ttgtgcagtt ctgattccga
     1741 cttacagccg gctgtagcgg aactgcgtga acgaaaaata gaaattatct atttaggttt
     1801 tgaagaaaat ccaaataagg gattaagtta tacaaccaac aggacaattc tcttgagaaa
     1861 ttcagaaatt ctcgccgcct cggcttccct ccccgccctc tccatccctg caagttgaca
     1921 aaaaattatt cagttttata atcatccgca tggaatggtt tagagctgaa ttagaaatag
     1981 atgaacccag ccgcataacc ggagagcc
//
LOCUS       DATDSU010000021         4162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_849641, whole genome shotgun sequence.
ACCESSION   DATDSU010000021 DATDSU010000000
VERSION     DATDSU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849641"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VI981_00590"
     CDS             <1..425
                     /locus_tag="VI981_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478843.1"
                     /translation="ADPKSFGSAVTVAMAQRLIRKLNPSKKKERPLTDEEKKMIAKVF
                     APLADKSLVPEKIETVWEPSPTSEDETGYKGRVGLYEAIFMDDELASFLRDNPPENEI
                     AKLATKQGYLTMAQDGIIKVLAGITSLSEVADTVDLPR"
     gene            complement(432..716)
                     /locus_tag="VI981_00595"
     CDS             complement(432..716)
                     /locus_tag="VI981_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEY4478844.1"
                     /translation="MRNLSTPSSSRCIHPKAPRPLTTREGARIQCFPDSYQFFGSRSD
                     RNLQIGNAVPTFLSNAIAGAILSNFKQEYAKEHRQVFKIKEKNATLSPVS"
     gene            complement(881..1624)
                     /locus_tag="VI981_00600"
     CDS             complement(881..1624)
                     /locus_tag="VI981_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEY4478845.1"
                     /translation="MKQELQKAMLIGMYLSKYDEQALSILGFESFVEAYNTIGLAINA
                     KPLSIRNYRDEFDPVFPNERKGWHKRPMFQTRVDMLNKYKDLNLEAFTDLVKNIIYKN
                     PAVNLIEEKIESEEDKSRTFAKRLITGQAAEQYFRDNYQSVPAFKGLAFEETTKFGCG
                     FDFRVLHNAGFYAVEVKGLENAKGGIGLTDKEYKTAFYLKESYFIFLVKNFIEVPFHI
                     LFRNPIFSHKLDFVKNEKTITQVSWNASV"
     gene            complement(1627..2427)
                     /locus_tag="VI981_00605"
     CDS             complement(1627..2427)
                     /locus_tag="VI981_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEY4478846.1"
                     /translation="MQRTNPITPEYFQQYRQKLGFTNQVDAKNFFGAKDIVPPVDFDY
                     LKLINKRLYEILGRINAVVSNEIKLADLNAFKKEYIERPFKVMKASGILPRLNNQGRR
                     PEDVYFNWMRGFVISNFFLKALGLIFEIDVADISLIGDDDFKSIETFKRTPKADISIN
                     LSKSEKIRIEMQSGFAGINDIKQHKILEAKRVFRDERAHSLAIHIDLYNGQAAFVKLD
                     EITDNDEHWMTRINLDEGLVYNIDQNFFFWDIKYVPPIYQHVTEKYYT"
     gene            2564..2848
                     /locus_tag="VI981_00610"
     CDS             2564..2848
                     /locus_tag="VI981_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEY4478847.1"
                     /translation="MKTAISFKIDRGVRNRARNVAKRIGVPLSMVVNQQLKQFADERC
                     IEFYEPLVPNAKTRKVLDEALRDIRTNRTDKFSPAFTNTRDMDRWLERNL"
     gene            2845..3108
                     /locus_tag="VI981_00615"
     CDS             2845..3108
                     /locus_tag="VI981_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HEY4478848.1"
                     /translation="MIVIYSKKFRRNYKKLPFKLQEQFKQRRRLFLANPFDLILNNHA
                     LHDPYVGCRSINITGDYRAIFYQENESVIHFLIIGTHHELFGT"
     gene            3311..>4162
                     /gene="ruvB"
                     /locus_tag="VI981_00620"
     CDS             3311..>4162
                     /gene="ruvB"
                     /locus_tag="VI981_00620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY4478849.1"
                     /translation="MSRSSSPIPPLPSSLDAALRPNNWDEYVGQKQVKANVRVGIDAA
                     KKRGGMPEHILFYGPPGVGKTTLAHLVAITLGSPLKSTSGVAIERAGDLAAILTNLEP
                     NTVLFIDEIHRLSRKIEELLYPALESGHLDLVLGKGPSARTVELALPPFTLVGATTRV
                     AELSGPLRSRFGGGVYQLDFYSDDDLRDIIRRSALLLNLKAPEEVVERIAARSRATPR
                     VANALLKRVRDFSEVHERKLSPALCDEACELFGIDTRGLTKDDRRYLETLLKSFRGGP
                     AGVRAIAA"
BASE COUNT         1108 a          979 c          895 g         1180 t
ORIGIN      
        1 gcgcggaccc gaagtccttc ggctcggccg ttaccgtcgc tatggcacag aggttgatac
       61 gtaagttgaa tccgagtaag aaaaaagaac gtcctctcac agacgaggag aaaaaaatga
      121 ttgcaaaagt ttttgcaccg ctcgccgaca agtctctcgt gccagaaaaa attgaaaccg
      181 tatgggagcc ctccccgacg agcgaggacg aaacgggata caaaggccgc gtcggcctct
      241 acgaagcgat ttttatggac gacgagctgg cgagcttctt gcgcgataat ccgccagaaa
      301 atgaaatagc gaaattagcg acgaaacaag gatatctcac tatggcgcag gacggcatca
      361 tcaaagtcct cgccggcatc acctccctct cagaagtcgc cgacacggtt gaccttccgc
      421 ggtaagtaca attaacttac tggacttagt gtggcattct tttcttttat tttgaagact
      481 tgtctatgct cttttgcata ctcttgtttg aaatttgaca agatggctcc tgctattgca
      541 ttcgacaaaa atgttggcac ggcattgcct atttgtaaat ttctatcgct tcttgaccca
      601 aaaaattgat acgaatcagg aaaacactga attcgcgctc cctcgcgcgt agtgagaggc
      661 cgcggggcct tcgggtgtat gcaccgagat gacgacggcg tgctcaagtt ccgcataacg
      721 gttgtcgatg gcctgtccca ccaaagacga caatatgtat ttttgaaacc ggatgtcggg
      781 cgcaactctt tcggcaaatc ttccggcgtt ccgccgtcag gcaaaagtta catcattttt
      841 accagttttg cgttattttt tggagcatta taatccatat ttatactgac gcattccaac
      901 tcacttgcgt aatcgttttt tcgtttttca caaaatccaa tttatgtgaa aaaattggat
      961 ttctgaataa aatatggaat ggtacctcaa taaaattttt taccaaaaag ataaaataac
     1021 tctctttcaa atagaaagct gttttgtatt ctttatcggt taatccgatg ccgcctttgg
     1081 cattttcaag acctttaact tctacggcgt aaaacccagc attgtgtaaa actctaaaat
     1141 caaatccgca tccaaatttt gtcgtttctt caaaagccaa acccttaaaa gccggtacgg
     1201 actgataatt atctctaaaa tactgttcgg cggcttgtcc cgtaattaat ctttttgcaa
     1261 aagttcttga tttatcttct tcagattcta ttttttcctc tatgagatta acggcaggat
     1321 ttttatatat gatattcttt accaaatccg taaacgcttc tagattcaaa tctttatact
     1381 tgttgagcat gtcaactctc gtctgaaaca tcggtctttt gtgccagccc tttctttcgt
     1441 taggaaaaac cggatcaaat tcgtctctat aattgcggat tgatagtggc tttgcgttta
     1501 ttgctaaacc gattgtattg tacgcttcca caaaactttc aaaaccaaga atgcttaatg
     1561 cctgttcgtc atattttgac aaatacattc ctatgagcat tgctttctga agttcttgtt
     1621 tcatatctat gtgtagtatt tttcggtaac atgttgatag attggaggca catattttat
     1681 atcccaaaag aaaaagtttt ggtctatgtt ataaaccaat ccctcgtcaa gatttatcct
     1741 tgtcatccaa tgctcgtcat tatctgtaat ttcgtccaac tttacaaaag cggcctgtcc
     1801 attgtaaaga tcaatatgaa ttgctaagga gtgcgccctt tcatcacgga agactcgttt
     1861 tgcttccaag attttatgct gtttaatatc gtttattccg gcaaaccccg actgcatttc
     1921 aattcgtatc ttttcgctct ttgacaaatt aatcgatatg tccgcttttg gtgttctttt
     1981 aaatgtttca atgctcttga aatcatcgtc acctattaaa gaaatatccg caacatcaat
     2041 ctcgaatata agtcccaatg cttttagaaa gaaattcgag ataacaaaac cgcgcatcca
     2101 gttaaaataa acatcttctg ggcggcgccc ttggttgttt agcctaggta gtatgccgct
     2161 ggccttcatc accttgaatg gtcgttctat gtattctttt ttgaacgcgt tgagatcggc
     2221 aagcttaatt tcatttgaaa cgactgcgtt tatacgaccg agtatttcat ataatctttt
     2281 gttgattagt tttaaataat cgaaatcgac tggtgggacg atatcttttg ctccaaaaaa
     2341 gttcttcgcg tctacctgat tagtgaagcc aagcttttgg cggtactgtt gaaaatattc
     2401 tggtgtgatg gggttcgttc tctgcatact cattcattat acacctctct ctagggaccc
     2461 tgtaaaaaca aaaattccgt ccatagtcct caaggcaaaa cccgcaatta agttgtgggg
     2521 ttgcttattg acatggcgta accgttgtat atacttcggt tatatgaaaa ctgccataag
     2581 ctttaagata gacaggggcg tacgaaaccg cgcgcggaat gtcgccaaga ggatcggtgt
     2641 gcctctatca atggtggtga accagcagtt aaagcagttc gccgatgaac gctgtattga
     2701 gttctacgaa ccactcgtcc cgaacgcgaa aacgcgcaaa gttcttgacg aggcattacg
     2761 cgatatccga acaaaccgca ctgacaaatt ctctcccgct tttacaaaca caagagatat
     2821 ggatcgatgg cttgagagga atctatgatc gtcatctact ctaaaaaatt tcggagaaat
     2881 tataagaaat tgccattcaa gttgcaggaa caatttaaac agcgtcggcg actatttctc
     2941 gcaaatccat ttgatctaat tcttaataac cacgcgcttc atgacccgta tgtcggttgc
     3001 agaagtatta atataacggg agactatcgt gcgatatttt atcaggaaaa tgagagcgtt
     3061 attcacttcc tcatcatcgg cacacaccac gaattattcg gtacataaaa caaaaatccc
     3121 gcccatagtc ctcaaggcaa aacccgcgat aaagcggggt ggacgaaaca cctgaggaga
     3181 ttcccagcga ccaataccga agtagtggac cagaaagtcc ttggggaaac gcccgaagcc
     3241 gtcaagcctt gccttgaaga gtatgaacag gaaagaaact atgatagaat ggtagcatta
     3301 tctaattttt atgtcaaggt cttcatcgcc tatccctccg ctcccttcct ctcttgacgc
     3361 ggcgcttcgt cccaacaatt gggacgagta tgtggggcag aaacaagtga aggcaaatgt
     3421 gcgtgttggt attgacgccg ccaagaagcg tggagggatg cccgaacaca ttctctttta
     3481 tggtccgccg ggggtcggta agacgactct cgcacacctt gttgctatca ctcttggctc
     3541 gccccttaag agcacttcgg gggtggcgat agagcgcgca ggcgacctcg cagccattct
     3601 cactaacctt gaaccaaata ctgttctttt catagacgag attcacagac tctcaagaaa
     3661 gatagaagaa cttctgtacc ccgcgcttga gtcggggcat cttgatttgg ttttggggaa
     3721 aggtccgtcg gcacgcacgg tggaactcgc cctccctccc ttcacgctcg tcggagcgac
     3781 gacacgcgtt gcagagctct ctgggccgct ccgttcgcgc ttcggcggtg gcgtgtacca
     3841 gctggacttt tacagcgacg acgacctgcg tgatattatt cgccgctcgg cgctcctgct
     3901 taatctgaaa gcccccgaag aagttgttga gcgcatagcg gcgagaagtc gcgcgacccc
     3961 gcgtgtcgcg aacgcgcttc tgaagcgcgt tcgcgatttc tccgaagtgc acgagcgcaa
     4021 actctcccca gcgctttgcg acgaagcgtg cgaacttttc ggcatagaca cgcgcgggtt
     4081 gactaaggac gaccgtagat atttggagac ccttctcaaa agcttcagag gtggccccgc
     4141 tggcgtccgc gccattgcgg cc
//
LOCUS       DATDSU010000022         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_355947, whole genome shotgun sequence.
ACCESSION   DATDSU010000022 DATDSU010000000
VERSION     DATDSU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355947"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VI981_00625"
     CDS             complement(<1..1017)
                     /locus_tag="VI981_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4478850.1"
                     /translation="MKIPAEVKNIVQKLQAAGYEAYIIGGSVRHLIVGESPNDWDLTT
                     NARPEEIQKIFSDSFYENNFGTVGVKTDSDDPTLQVIEVTTYRIESKYTDKRHPDDVM
                     YADRLEDDLRRRDFTMNALAMSEDGSVTDLFGGQGDIKNKVIRAVGDPNERFNEDALR
                     MMRAVRFVAQIGFTIESGTLEAIKKNNGLIAAVSKERIRDELLLLIDSPDADSGIEIL
                     RETGLMKFVMPELLEGVGVAQNKHHIYTVWEHNIKALRYACDKGYSAEVRLASLLHDV
                     GKPRSKRGDGHDSTFYGHEMIGANMTAQVVDRLRLPLKQAEKIIKLVRYHLFYYNVGE
                     VTESS"
     gene            1061..1135
                     /locus_tag="VI981_00630"
     tRNA            1061..1135
                     /locus_tag="VI981_00630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1094..1096,aa:Arg,seq:cct)
     gene            1148..1218
                     /locus_tag="VI981_00635"
     tRNA            1148..1218
                     /locus_tag="VI981_00635"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ala,seq:ggc)
     gene            1639..2100
                     /locus_tag="VI981_00640"
     CDS             1639..2100
                     /locus_tag="VI981_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478851.1"
                     /translation="MPNTDFTYEGFEGLLASFYLSLLPLHDSGDVLDKQDFETALGIL
                     NGFAKRHKVQKPQNAVAHTRPTSEEWGQPKKFDSCFTLLDMMGSFWRDFGVGTDPKKL
                     DGQERNKLGRLLIGLLDRHGVLKAKFDTLDGQQVAVGVESWTKDREFQSLA"
BASE COUNT          616 a          556 c          508 g          631 t
ORIGIN      
        1 tgacgattcc gtaacttcgc caacattgta atagaaaaga tgatatcgca ccagttttat
       61 gattttttca gcttgcttaa gaggaagcct gagcctgtcc acaacctgtg ccgtcatatt
      121 ggcaccaatc atttcatggc cataaaaagt tgagtcgtgg ccgtcgcctc ttttacttcg
      181 tggtttgccg acgtcgtgta aaagtgatgc taagcgcact tcagcggaat agcccttatc
      241 acatgcgtat ctcaatgctt ttatattatg ctcccacact gtatatatgt gatgcttatt
      301 ctgggcgaca ccaacacctt caagcagttc gggcataaca aatttcatta atcccgtttc
      361 gcgcaatatc tctatcccgc tgtctgcgtc cggcgaatct atcaacaaca gcaattcatc
      421 gcggattctc tccttagaaa ctgccgcaat gagtccatta ttttttttaa ttgcctctag
      481 cgtgccggat tctatagtaa atccgatctg ggcgacaaac ctaaccgccc tcatcatacg
      541 aagcgcatct tcgttgaatc tctcgtttgg atcgccaact gcacggatta ctttattttt
      601 aatatctcct tgcccgccaa acaaatcagt aaccgagccg tcttcactca tagcaagggc
      661 gttcattgta aaatcacgtc tccttagatc atcttcaagc ctgtcggcat acattacgtc
      721 atctgggtgt cgcttgtcgg tatatttgga ttctatgcga tacgttgtga cttcaattac
      781 ctgcaaggtc ggatcgtcac tatctgtttt aacgcctacc gtgccaaaat tgttttcata
      841 aaaactatca gaaaaaatct tttgtatttc ttccggcctg gcattcgtag tcaaatccca
      901 atcattcgga gactcgccca cgatcaaatg ccgcacgctc ccgccgataa tatacgcttc
      961 gtaaccggca gcttgtagtt tttgaactat atttttgacc tctgcgggaa ttttcatatt
     1021 gctaaaataa catttttttg ctacttttac aacgaaaaca gcccctgtcg ttcaacggat
     1081 aggacgaggg tttcctaaac cctaaatcca agttcgactc ttggtggggg caccaaagtt
     1141 taaaaacggg cctgtagtaa aacggtatta cacatccatg gcatggatgg ggtcggggtt
     1201 cgattccccg caggtccaca aattaaaaca gcttttaaag ctgttttaat ttgtggagat
     1261 gagcgagcaa agagttttgc tcgcgttggg gaggagaacg gcggagcatg tggtgaatcg
     1321 caattgagac acgaaggtgt cgaaatactg agcataacaa tgcgaacgta ttggcggtga
     1381 gccacgcgag caggtggcta gcccgagctg gcggaagcca gcgagaggcg aagccgactc
     1441 cctgcgtcat cacaaaatta gccataaaat catataggtt ttctgcgaca aaaatcaccc
     1501 gaaaatggtg atttttggtg ggtaaaagac tagagttgaa tgtgccgccg taatttgctt
     1561 gacttttgcc gtatagctat ggtatagtta agttatagtt ccaatcacaa tcaggagaca
     1621 accatgaaca aaggaagtat gcccaacacc gatttcacct acgaagggtt cgaggggttg
     1681 ctagcaagtt tttacttatc attgcttcca ctgcatgata gtggtgatgt tctcgacaaa
     1741 caagatttcg aaaccgccct cggtatcctc aacggctttg ccaaacgaca caaggtccag
     1801 aaaccccaga acgcagtcgc tcatacgcga ccgacctcgg aagagtgggg tcaaccgaag
     1861 aagttcgatt catgcttcac gcttctcgat atgatgggtt cgttctggcg tgacttcggc
     1921 gtcggtaccg atcccaaaaa attggacgga caggagagaa acaaactcgg ccgtctacta
     1981 atcggcctac ttgaccgcca cggtgtcctc aaagccaagt ttgatactct cgacggccaa
     2041 caggtggccg ttggtgtcga aagttggaca aaggacagag agtttcaatc tctcgcctga
     2101 acctcgcaca aaagacagga gacctcaatc ctcgtctggt gcgaggtctt ctgtttttat
     2161 acggaaattg cattgtatgc attttgcaat aaagcgacgg tatttaatga aatcctggta
     2221 tttgcccagt cttttatgta ttcaaacatc gtcaacgaac ttccacaatt ccgaatttga
     2281 agaaagattg acctcgcccg cgccaaaggc g
//
LOCUS       DATDSU010000023         6827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2361732, whole genome shotgun sequence.
ACCESSION   DATDSU010000023 DATDSU010000000
VERSION     DATDSU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361732"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VI981_00645"
     CDS             complement(<1..263)
                     /locus_tag="VI981_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478852.1"
                     /translation="MPCAKRRLEQSNPPAGGYVCIFKAQFDRPLYAFFQPSEPSLFEN
                     LARSNWKQMSRVENCLWLRERSDLGNFRNRGDYCFLIAEAVFS"
     gene            483..1412
                     /locus_tag="VI981_00650"
     CDS             483..1412
                     /locus_tag="VI981_00650"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY4478853.1"
                     /translation="MTKKIIGSLIVASLILGGSTAAYSKEKNGKEKGDDNDYKFKRSL
                     SRGHRGDDVRALQAFLALDTDVYPEGLISGIYGELTEKAVKRFQKKNGIDAVGIVGPK
                     TRMFIEIALSNGSSLTVRLSPGMARKLGITATTTVASSTIVTKSANDRPTTTPVSDHK
                     IVLCHKPGSAQQTLTVGIPALIAHLKHGDSIGVCVGTTTPPIPPPTPPASDVTAPLIS
                     SLNGSAISSTTATIGWQTNEPADGMLWYGTTSPINPFAVNVTNVGTSTLTLAHTFTIG
                     GLTASTTYYYLVRSKDASNNTATSTSQTFQTID"
     gene            1606..2538
                     /locus_tag="VI981_00655"
     CDS             1606..2538
                     /locus_tag="VI981_00655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY4478854.1"
                     /translation="MKAAQITNYGEKEVVIISNIPQPVARAGEILVKVHAASVNPIDW
                     KIQAGYLAKMAPLTFPKTMGMDLAGVVEQVGESSSEFHPGDVVYGQSVFFGAGGAFAE
                     YAICIAKGLAGKPTNVTYEEAAALPLTGVSAIQAITEHIALKSGQKILVHGGAGGIGS
                     MAIQIAKNIGAYVATTASKSEADYVKALGADEIIDYESQKFEEVLKEYDAVYDTVGGE
                     TYIRSYKVLKEGGVLVSMIEKPTFALNQQYNVKAISQNTELNTDRLITLKHLVESGVV
                     KVHIDKIFTLDESGEALLHLKKNHPRGKVVIRVK"
     gene            3171..3440
                     /locus_tag="VI981_00660"
     CDS             3171..3440
                     /locus_tag="VI981_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478855.1"
                     /translation="MLARIGIPALIVLALVGGAWFRRVPRAAIKKEQNKQVEMKESQK
                     LPTKDSSDSSLDKDIASIEAEIKLLESEDAMVDQGLGDKPISQEE"
     gene            3445..4236
                     /locus_tag="VI981_00665"
     CDS             3445..4236
                     /locus_tag="VI981_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478856.1"
                     /translation="MKSILKSLIVIIVGVSIILSATPALAQVGSAATGTPGSKMTVEQ
                     RIAKRKEKANQEINRRLASLAKLKTIIQNMKHITDTQKASLTATIDAQVASLTALKTK
                     IAADTDLNTLKTDQKQITQSHRIYALVLPQVQILAAANRALNVSELLMALTLKLEERI
                     NKAASEGKAVTSLQTALTDMKAKIADAKVQAEAAITAVVSLTPDNGDQAKATLNRQAL
                     LDGRAKLKAARVANNAAKQQAQVIRQGLRGFGTATTTPATPPVTP"
     gene            4376..4723
                     /locus_tag="VI981_00670"
     CDS             4376..4723
                     /locus_tag="VI981_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478857.1"
                     /translation="MNSPLNSSLSSGSSSENILSRLSHHKKILVLVIGAVVILVLSMV
                     AFYLSHQKVKNEAEKIAREAELVRNLSSSLESSSASEINVPSTNPAKSAVPSVDPSEK
                     TNPFNDEYENPFE"
     gene            4695..6629
                     /locus_tag="VI981_00675"
     CDS             4695..6629
                     /locus_tag="VI981_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="resistance to Congo red protein"
                     /protein_id="HEY4478858.1"
                     /translation="MMSTKIHSNNFAALAIVIVAFFGATLIGVLNARAQSSITYPVVE
                     LGNCENKADCRSYCDVDGPFSDDLSDEERSQRRSACQNFASQHRKSNVRNLENANKIN
                     DKLNSIKKDGGPGGECGKAENPIAACKAFCDRSSNIPVCVEYAKKHGLFEGKELEEAE
                     KVARALERGIKLPASCKDSDSCQSVCENPKDVTVAKQCFAFAKEAGLLPPGFDEGKAE
                     KFFQAMENKNGTLNFQDMRKCERPENDDVLEKCIQIGLDSGFITAEQAEMMKRTKGKG
                     PGGCRGEECRTYCETEEHQEECMRFSEEHGLMGEEEKARQQREMDQFRKFLSEAPEKV
                     KTCLKDTLGEAVLNDLQSGAKRPSRDLGEKMRTCFDNYFRSRDNMSHDEFNGDGGDMN
                     RRNNMRGMMDSESREYSDRPMEPQEGTNSNKGVPMMRGNSMPNRFPDVVVKCVESKVG
                     AEVARKLADPRTGRPSPEQEKIIGECMTILRKQGLMQGNSTSERSPTRPYQGGTNMPN
                     GDYGPDSHMTPEEMRAKYESGSGGSYRGEFNKPPVGDYPNQMPAPGSYRPSPEEMQRY
                     NQNSTGGIYQHPEMNQEMPPSTSGSYIQPPPDQYSPESGSYENVTEPQMSPPPLETSP
                     TTRRRFNMLMSNILYALIGL"
BASE COUNT         2081 a         1445 c         1652 g         1649 t
ORIGIN      
        1 tttgaaaaca ccgcttcagc aataaggaaa caatagtcgc ctcgattacg aaaattgcct
       61 aagtcgcttc gctcccgaag ccagaggcaa ttttctactc ggctcatttg tttccaattg
      121 cttctcgcaa ggttttcaaa aagagaaggc tcgcttggtt gaaagaatgc ataaagaggg
      181 cgatcgaatt gcgccttgaa aatgcacaca tatccgccag ctggcggatt gctctgctca
      241 agccgccgct ttgcgcaagg cattttcaag gtgcaattct cgctcgatcg tgtgatttca
      301 agtttgggtc ttgaaatgcg gcaagaggac tccgaatttt gatcagggga gcacacttcc
      361 ttgcaaaagt cttggtaaga acgtggttga ggcggcgtct atgaaagcga ccgcatgggc
      421 gggtttagag ctataatcat taacgcgttt agattaacaa ttcactcata tacatattta
      481 gaatgactaa aaaaattatt ggttctctaa ttgtggcaag tcttatattg gggggatcga
      541 cagccgcata ctctaaagaa aagaacggca aagaaaaggg cgacgacaat gattataaat
      601 ttaaacgatc tctgtctcgg ggtcatcggg gagatgatgt cagagcactg caagcgtttc
      661 ttgcgcttga cactgatgtc tatccggaag gacttatatc ggggatctac ggcgaattga
      721 ccgaaaaagc agtcaaacga ttccaaaaaa agaatggcat tgatgctgtc ggtatcgtag
      781 gaccgaagac gaggatgttt atagaaatag ccctatctaa cggttcgtca cttacagtgc
      841 ggctttctcc gggtatggcg cgaaaattag ggataacggc gacgacaact gtagcctcaa
      901 gcacgattgt tacgaaatct gcaaatgatc gaccgacaac gacgccagtc tcggaccaca
      961 aaattgtcct atgccacaag cctggatctg cgcagcagac acttacagta ggcattccag
     1021 ctctgatcgc tcatcttaag catggtgatt cgatcggtgt atgtgtcgga acaacgacgc
     1081 ctccaatacc acccccgaca cctcccgcct ccgacgtgac cgctcctcta atttcttcgc
     1141 ttaacggaag tgcaatttct tcgacgacgg cgacaatcgg ctggcagact aatgaacctg
     1201 cagatggaat gctttggtac ggcacgacat ctcccataaa tccttttgca gtgaatgtaa
     1261 cgaatgttgg cacgtctacg ctcacactgg ctcatacctt tacaataggg gggcttacgg
     1321 caagcacgac atactactat ctagttcgtt cgaaggacgc ttcgaataat acggcaacaa
     1381 gcacaagcca aacattccag acgatagatt agtttccgaa tattaaacga aacacaaaac
     1441 tgtccaaagg gcagttttgt gttggggaaa atctgtgtaa aacctgtggc taatgtgggg
     1501 agaaaaaggg gataagaatg cttgtaattt aaaaaaattg ccttcgcgta agccccagtg
     1561 taatatatag ataagtttcg atacttaatc gcctatttta aaaaaatgaa agctgcacaa
     1621 ataacaaact atggagaaaa ggaagtggta ataattagca acattccgca acctgttgca
     1681 agagcggggg agattctagt taaagttcat gcggcaagcg taaatccgat agactggaag
     1741 attcaagcag gatacctcgc caaaatggcg ccgctcacat ttcccaaaac aatgggcatg
     1801 gatctcgcgg gagtcgtcga gcaagtcggc gaaagttcgt cagaattcca cccgggagac
     1861 gtcgtgtacg gacagtcagt attttttgga gctggaggtg cgtttgctga atatgccata
     1921 tgtatcgcta aaggattggc aggtaaacca accaatgtaa cttacgaaga agcggcagct
     1981 ctgccgctca ctggcgtaag cgctattcag gcaatcacag aacacatagc tctaaaatcc
     2041 ggccaaaaga tcctcgtcca cggcggggcg ggaggcattg gcagtatggc cattcaaatt
     2101 gcaaagaata ttggagcata tgtcgcaaca actgctagca aaagtgaggc cgattatgta
     2161 aaagcactcg gcgcggacga aataattgat tacgagtctc aaaagtttga ggaggttctc
     2221 aaagagtacg atgcagtcta tgacactgtc gggggtgaga catacattag atcatataaa
     2281 gttctgaaag agggtggtgt tcttgtgagt atgatcgaaa aacctacatt tgctttgaat
     2341 cagcaataca atgttaaggc catttcgcag aataccgaac ttaacactga ccggctcata
     2401 acacttaaac atcttgtaga aagtggtgtc gttaaggtcc acattgataa aattttcaca
     2461 ctcgacgaat caggagaagc actcctgcat ctcaaaaaaa atcatccgcg aggcaaagtt
     2521 gtgatccggg taaaatgata agatattctc tctattgaga actatggaat tcctgacgag
     2581 gatcagagca aaagcactgt tttacattct ttttattttg gctagcgagg cgagaagctc
     2641 cgggggagct tcgcaagccc ttttgaactc aagtcttcga gagccaaaag gtgtacagct
     2701 tctgaaagaa gcgtgtgtcc gcctcaggcg gacacatcgt ccgagccccg cccgtaccga
     2761 acggtacgtt cgggcgggga agacaaaata tatgattgga gtcacatata attgcgtcat
     2821 tgattaccct gcagtctttg ccacatggcc tatatttgcg gcccgcaggt tttgctcact
     2881 ctttttccaa aaagtttgag ggcccgcggc gttcctaaat ctcgcatcca tttcgagctt
     2941 tttcgcttgt attaagtgac gaacttatgt taaaaacgga gatctcggtg aatgagcaga
     3001 cgaggtagca agcacccgcg gcgtttttgt ggctttgagc agaatcgccg actgattatt
     3061 gagagtagga catcctactt ttctttgagg ctttggggta aagcgttata cttaaacgca
     3121 tatgttgtag ggttataacc cccgtcattt atcagtaaaa ataatttttt atgttagcac
     3181 gcataggaat tcctgccctt attgttctcg ctcttgtggg aggtgcatgg ttcagaaggg
     3241 taccgcgagc cgccattaaa aaagagcaaa acaagcaagt cgagatgaag gagtctcaaa
     3301 aattgccgac aaaagattcg tcggatagtt cgcttgataa ggatatcgct tcgattgaag
     3361 ccgagataaa actgcttgaa tcagaagatg cgatggtcga tcaagggttg ggcgataagc
     3421 cgatttctca agaagaataa atttatgaaa tcaattctaa aatcactgat cgtaatcatc
     3481 gttggtgttt cgattattct ttctgcaacg ccggcgctag ctcaagtcgg aagtgctgcg
     3541 actggcacgc cgggttcaaa aatgacagta gaacagagaa tcgccaagcg aaaagaaaag
     3601 gcaaatcaag aaataaaccg acggctcgct tcgctcgcca agttaaagac catcatccaa
     3661 aatatgaagc atatcaccga tacgcagaaa gcgtcactta cggctacaat agacgcacaa
     3721 gtggcttcgt tgactgcgct taagactaaa attgcagctg acacagatct aaatactctg
     3781 aagacggacc aaaaacaaat tactcaatca caccgcattt atgcgctcgt tctgccgcag
     3841 gttcaaatat tggcggcagc taacagagct ctaaatgttt cagagttgtt gatggcgctt
     3901 accttgaaat tagaagagcg aataaacaaa gccgcttccg aaggcaaggc cgtgacatct
     3961 ctacaaacgg ctcttaccga catgaaagca aaaatcgcgg acgctaaagt tcaagcagaa
     4021 gcggcaatta cggcagtggt gtcgcttact cccgacaacg gagaccaagc taaagcaact
     4081 ctaaatagac aagcgctgct tgatggcagg gctaagctta aggcggctcg ggtggcaaac
     4141 aacgcggcaa aacagcaggc gcaagttatt cgtcagggct tgagagggtt tgggacggca
     4201 acgacgactc cggctactcc gccggtgaca ccgtaaagag agtcaagaaa caaacaaaaa
     4261 atccgcttca taggcggatt ttttgtttca gacaagcagg caaatgcgta tgtgtataac
     4321 ttaacaaata aaatttcgag cgtgatataa tttatcagtt gttattctcc attttatgaa
     4381 ttcaccactc aattcatctc tgtcttctgg gtcctctagt gaaaatattc tgtctcgttt
     4441 gtctcaccac aagaaaattt tggttctggt gataggtgcc gtggtcattc ttgttttaag
     4501 catggttgct ttttatcttt cgcatcaaaa agtaaaaaat gaggccgaga aaattgcgcg
     4561 cgaagcggag cttgtaagaa atttgtcatc ttcgcttgaa agttcatccg cgtcagaaat
     4621 aaacgtgcct tctactaatc ctgctaagtc cgcagtcccg tctgtggatc caagcgaaaa
     4681 gaccaatcct tttaatgatg agtacgaaaa tccattcgaa taatttcgcg gcactggcca
     4741 tagttattgt cgctttcttc ggggcgacac tgattggagt acttaacgcg cgcgcacaat
     4801 catcgatcac atatcctgtg gtagaacttg gtaactgcga aaacaaggct gactgccgat
     4861 cgtattgtga tgtggacggt ccattttctg acgatttaag tgacgaagag cggagccaaa
     4921 gaagatcggc atgccagaat tttgcaagcc aacaccgaaa atccaatgtt cgtaatttgg
     4981 aaaacgcaaa caaaataaat gacaagttga atagcataaa gaaagacggt ggacctggtg
     5041 gagaatgcgg aaaagccgag aacccaattg ccgcatgcaa agcgttttgc gataggagtt
     5101 ccaatattcc agtatgtgtt gaatatgcaa aaaaacacgg tctctttgaa ggtaaggagc
     5161 ttgaagaagc tgagaaagtt gcccgtgcac tcgagcgagg tataaaactg cccgcatcat
     5221 gtaaagactc tgactcttgt cagtctgtat gtgaaaatcc gaaagatgtg acggtagcga
     5281 agcaatgttt tgcttttgca aaagaagcag ggcttctgcc ccctggcttt gatgagggta
     5341 aagcagagaa atttttccaa gcaatggaaa ataaaaatgg gacacttaac ttccaagaca
     5401 tgcgcaagtg cgaaagaccg gaaaatgacg acgtactaga aaaatgtatc caaataggct
     5461 tagacagcgg cttcataact gccgaacagg cagaaatgat gaaacggaca aaaggaaaag
     5521 gtccaggcgg atgccgtggc gaagagtgcc gaacgtattg tgaaacggag gaacatcagg
     5581 aggagtgcat gagattttct gaagaacatg gtcttatggg ggaagaagaa aaggctaggc
     5641 aacagagaga gatggatcaa tttcgtaaat ttctctcgga ggctccagag aaagtaaaaa
     5701 cctgcctcaa agatacgttg ggagaggcgg ttttgaacga tcttcaaagt ggggcaaaaa
     5761 gacctagccg agatttagga gaaaaaatgc gaacgtgttt tgacaactat tttcgatcga
     5821 gagataacat gtcgcatgat gagtttaatg gagacggagg cgatatgaat agaagaaata
     5881 acatgcgtgg gatgatggat tccgaatcga gggaatattc tgatagaccg atggaaccgc
     5941 aagagggcac aaatagtaat aaaggagtgc ctatgatgag gggtaattca atgcccaacc
     6001 gctttcccga tgtagtagtg aagtgtgtag agtctaaagt cggtgcagaa gttgcacgta
     6061 aactcgcaga tccaagaacg ggacgaccaa gtcctgaaca ggaaaagata attggcgaat
     6121 gtatgacgat tttgcgtaaa cagggactta tgcagggtaa ttctacttct gaacggtctc
     6181 ccacgaggcc gtaccaagga gggacgaaca tgccaaatgg agattatggc cctgattcgc
     6241 acatgacgcc agaggaaatg cgtgcgaaat acgaaagcgg aagtggcgga agctatagag
     6301 gagaattcaa taagccgccc gttggcgatt atccaaatca aatgccggcg cccggttcct
     6361 accggccatc acctgaagaa atgcaacgat acaatcaaaa ttctacagga ggcatatatc
     6421 agcatccaga aatgaatcaa gaaatgccgc catctacttc tggatcctat attcaacctc
     6481 cccctgacca atattctccc gaatcaggtt cctatgagaa cgtgacggaa ccgcaaatgt
     6541 caccaccacc tctggaaact tctccaacga cacgacgacg tttcaatatg ctaatgtcta
     6601 acattctcta cgcgctcatc ggtctttaga aatattattt gaggacaatt attttgtcct
     6661 agcttgattt ttcgactttt gacttgtcca ccccagtccg cctcaggcgg acggggcgag
     6721 aagtggttac gtactaaaaa tatttagcgg tgcgttcagt tgtagcttca ataatcatga
     6781 agcgagaagt ggttacgtac taaaaatatt tagcggtgcg ttcagtt
//
LOCUS       DATDSU010000024         4693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1190148, whole genome shotgun sequence.
ACCESSION   DATDSU010000024 DATDSU010000000
VERSION     DATDSU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190148"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..274
                     /locus_tag="VI981_00680"
     CDS             56..274
                     /locus_tag="VI981_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478859.1"
                     /translation="MYVSKEHFRNDFFNTLLFSRRGVYFTSKRVVLITTTKPYGNHED
                     TDAKPKDSGKRSKHTWRAGRQDHNSVLH"
     gene            909..1241
                     /locus_tag="VI981_00685"
     CDS             909..1241
                     /locus_tag="VI981_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478860.1"
                     /translation="MSHITYSRLLSRIAGVVITTDILMFTLILFLPNPGDPPYKMKAL
                     IGFTVAAFLLKCLAFATAHDNGYRNDGGNDYDPLPPIRPCPRHGKRRRRFDRLRYEEF
                     RFPPAHKR"
     gene            complement(1375..2079)
                     /locus_tag="VI981_00690"
     CDS             complement(1375..2079)
                     /locus_tag="VI981_00690"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY4478861.1"
                     /translation="MKIKPIRTRIFREGENLISFILKYLPRVKEGSVIAITSKIVALA
                     ERRTVVLENTNTKRDLIIRESEIAVETRHAWLTLKDGMIMPSAGIDESNANGKLIQLP
                     KDSFKSAAGLRRLLNKHYKTNDLAILITDSRTTMLRAGTVGVALGYAGFQGIRDYRNK
                     RDIFGRKFIFQRANIADGLASAVVLTMGEGNEHCPLAVVTKAPVQFCKKVDKKELYIP
                     LKDDMYGPILGKFKKS"
     gene            complement(2060..2716)
                     /locus_tag="VI981_00695"
     CDS             complement(2060..2716)
                     /locus_tag="VI981_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY4478862.1"
                     /translation="MITLSQKSLTQLLMALAIYLTSLLAANTLGLKIMPFLFGSHLSV
                     AVFSFPVVFLSTDVIGEVYGRKMSKLFVRAGFASTALFIGFSLISVLMPWSQDGLWAK
                     ESYNQIFGTSARIAIASLVAFLVAELQDVFSFFLLKEKLGIKRFWIRSWISNIWSQFL
                     DSALFMLIAFAGIYSWKTLFSIVITWWLYKVAMGILYTPLSYVGLRLLKDTANENKTN
                     "
     gene            complement(2919..3443)
                     /locus_tag="VI981_00700"
     CDS             complement(2919..3443)
                     /locus_tag="VI981_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4478863.1"
                     /translation="MLKEEIDKIINSGVALEATKASFAKRLGEGRLTRDENSASHFCV
                     YFLPFNRAKKEVFVIHHKKARLWLSPGGHIDQGETILETLNREIKEELGVPAFFKALP
                     EPFFFEVTPIENSIQTCKFHFDIWYLMQTDGQKFNVDSSEFLDTRWLRFNEARKIVSD
                     PANLKALNLVEEMF"
     gene            3529..3975
                     /locus_tag="VI981_00705"
     CDS             3529..3975
                     /locus_tag="VI981_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478864.1"
                     /translation="MKFYLFLRRVLNFTIFGFCLGGLGALAAILFQFVKVRAETGVNL
                     GFIDPDVNFILTACIYTAGIGAIAGAIWKIFEKRGPIGIGFLGGSLAGLIGMVPNIVS
                     AGAKTVDWDVPLAYSLLFGFFGGMFGWFFCRLRKEIGAGRDRRRSK"
     gene            4046..4396
                     /locus_tag="VI981_00710"
     CDS             4046..4396
                     /locus_tag="VI981_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478865.1"
                     /translation="MNYESEVLDRPPVAEDEVVRALQDDGIENADAKELLMQLAEQYQ
                     READTEDTSLANISAALKLAKIYARAGMADDALRSLEGLREAAFHEREASNVRFTEIS
                     RVIDDIESGGVWKA"
     gene            4471..>4693
                     /locus_tag="VI981_00715"
     CDS             4471..>4693
                     /locus_tag="VI981_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY4478866.1"
                     /translation="MLGTLNRLVLSAVEGKRGSISRMSIKVYRFETLGELTAELYRSI
                     YYYNNLRIHTSLGMAPKKFTEKFAQEKEIK"
BASE COUNT         1318 a         1038 c         1039 g         1298 t
ORIGIN      
        1 tcgcttggtt aacagagaga aaattcaaaa cacgagaaat ttttcagaat aaattatgta
       61 cgtttcgaaa gagcactttc ggaacgactt tttcaacaca ttgctttttt caagacgagg
      121 agtatatttt acttcgaaac gagtcgtgct cataaccaca accaaaccat acggaaatca
      181 tgaagacacc gatgcaaagc ccaaggattc gggcaaacgc tccaagcata cctggcgcgc
      241 tggaagacag gatcataact ccgttctgca ctgacgagta cagtattttg gctcgaattg
      301 ccggcaacac ggcaaagcca acaagactgt ggctctctac atgcagacag attgcgattc
      361 cgtttaacag tgtattcggg catttggcgc gcttggccgg tgttcccagt acttcagatc
      421 cgtcacaccg tacttttgct gtgtgagctt gtgtccataa ggaggactga tttcgatcgg
      481 acctcccttt ttttatcccg ttagaagcaa aataccccgc gatctctgtc gcgaggatga
      541 atgctaattt agaacagcga acacggggtt gaatacccct ctgtgagctt ctaacgggat
      601 ttatctaaca attcccttgc gattttactg ccacgagtta tagtaatctc ggccgaaacc
      661 cccacaaaca gaaatgcaac tatgcgacct gatcatcgaa aaccctggtg ttacaaatac
      721 cctattatca aagcgttgtt ccggcggcgc atgccgaagg agcaccgttt tcttgggcgc
      781 tttggacgcc gttgttgatt cgatgtcgtt agcgtgagcg ggaaaccgct catttttgtt
      841 tctctcgatt tgagcgtgct accgtcattg accaaatgtt aataaaggcg tagccttgtc
      901 cgcagaatat gagccacatt acctactcac gtcttttgtc gcgcatcgct ggtgtcgtta
      961 tcacgaccga catcttaatg tttacgctga ttctcttttt gccaaacccc ggcgatccac
     1021 cgtacaaaat gaaagcgctt atcgggttca cggtggccgc ttttttactc aagtgtttgg
     1081 catttgccac agcacacgat aatggataca ggaatgatgg cggaaatgac tacgaccccc
     1141 taccaccgat acggccatgt ccgcgtcatg gaaaacggcg ccgacgtttc gaccggctcc
     1201 gatatgagga attcagattt cctcctgcac acaaacgtta agcacctgca ttgcagggct
     1261 ttttttattc tagatagcga caaaagtgtg cccgcctgag gcgggctagg aacgagtctt
     1321 cgagtagcct ctattacccc ggggtaaagc ctcgggaatc tatattcgcc agttttaact
     1381 ttttttgaat tttcccaaaa ttggtccgta catatcatct ttaagaggaa tgtataactc
     1441 ctttttatca acttttttgc agaattgaac tggcgctttt gttacaaccg caagtgggca
     1501 gtgctcattc ccctcgccca tcgtcagtac gacagcggag gctagtccgt ctgcaatatt
     1561 cgctcgctga aaaataaatt ttctcccgaa gatatctctt ttgtttcgat aatctcgaat
     1621 tccttgaaaa cctgcatacc caagagcaac cccgaccgtg ccagcgcgga gcattgttgt
     1681 acgagaatcg gtaataagaa tagcgagatc attagtttta taatgtttat tcagcaaacg
     1741 cctgagccct gcagccgact taaaagaatc ttttggaagc tgaattagtt ttccatttgc
     1801 atttgactcg tcgatccctg ctgacggcat aatcattccg tctttcaatg tgagccaagc
     1861 atgtcttgtc tcgacagcaa tctcactttc gcgaataatg agatcccgct ttgtattggt
     1921 attttccaac acaacagttc gtcgttcggc aagtgcaaca atcttcgagg ttattgcgat
     1981 cacacttccc tcttttactc ttggcagata ttttaagata aaggaaatta gattttctcc
     2041 ttctctgaat attcgagttc taattggttt tattttcatt cgcagtatct tttagaaggc
     2101 gcaagccaac atatgaaaga ggcgtataaa gaatgcccat tgctacttta tagagccacc
     2161 aggtaataac gattgagaaa agcgttttcc atgaatagat accggcgaac gcaataagca
     2221 taaagagggc ggagtcgaga aactgagacc atatgtttga aatccatgat cgaatccaaa
     2281 aacgttttat gccaagtttt tctttaagca gaaaaaagga aaatacatct tgaagttcag
     2341 caaccaagaa agcaactaga gacgcaatag caatacgggc cgaagtcccg aatatttgat
     2401 tgtacgattc ctttgcccaa agtccgtcct gtgaccacgg cataagtaca gaaatgagag
     2461 aaaagccgat aaaaagtgcc gtactcgcaa agccggcccg gacaaagagc ttggacattt
     2521 ttctgccata tacttctcca ataacatcag tagagagaaa aacgactgga aaggaaaaca
     2581 cggcaaccga caaatgggaa ccgaagagaa acggcatgat tttaagacca agcgtatttg
     2641 ccgcaagaag cgatgtgaga tagatagcta aggccattaa gagttgcgta agtgattttt
     2701 gtgataacgt gatcatttaa gtgtaactag gttatataca ttaactttac gcacgtgcgt
     2761 aaagttaatg tatgtggcgt tttagtaata ttgaatagta aaaagccccg caattggcgg
     2821 ggcttttcgg tacatcgaaa atacccgcct agtcgatcag ccgtccagta aatcctgacc
     2881 agattgattt atcgttagta agaagcatga gagttatatt aaaacatttc ttcaaccaga
     2941 ttcaaagctt tcaaatttgc cgggtcagag acaatttttc gggcttcgtt gaaacgtagc
     3001 caacgcgtat cgagaaattc agacgagtct acgttgaact tttgtccgtc agtctgcata
     3061 aggtaccaga tatcgaagtg aaatttacac gtctgaatag agttttcaat aggcgtaact
     3121 tcaaaaaaga acggttccgg caacgcttta aaaaaagccg gcacacccaa ctcttctttt
     3181 atttccctgt ttaaggtctc gagaatggtt tcgccttgat caatatgtcc tcccggagaa
     3241 agccagaggc gggccttttt gtgatgaata acaaaaacct cttttttcgc acggttgaat
     3301 ggcagaaaat atacgcaaaa atgcgatgca gaattttcat ctcgagtcag tctcccctct
     3361 cctagacgct ttgcaaatga tgctttcgtt gcctcaagag cgacacccga gttgattatt
     3421 ttgtcgattt cctccttcaa catactcaat cttataatta tacttgattt gattttgcct
     3481 gcggctatga ttaagcagga tactttcggt aaacttcttt caagcactat gaaattctac
     3541 ctgttccttc ggcgagttct caacttcacg atttttgggt tttgtctcgg cggtctcggc
     3601 gcattggcgg caatcttgtt ccaatttgta aaagtcagag cggaaacagg tgtcaatttg
     3661 ggattcatcg acccagatgt caatttcatc ctgactgcct gtatttatac cgccggtatt
     3721 ggtgcaatcg ctggagcaat ttggaaaata ttcgagaagc gcggacctat tgggattggt
     3781 tttttaggtg gttcgcttgc tggtcttatc ggtatggtcc caaacattgt ttctgccgga
     3841 gcgaaaactg tagactggga cgttccgctt gcttactctc tgcttttcgg attttttgga
     3901 ggcatgttcg gctggttttt ttgccgactt agaaaagaaa taggcgctgg aagagaccgc
     3961 cgacgatcga aatgagcatc ataatgatgc tttttttaat tttcacaatc gtggacagaa
     4021 aatattagga gggtacaatt catgtatgaa ctacgaatca gaagtcctag atcgcccacc
     4081 tgtcgccgag gatgaagttg taagagctct tcaggacgat ggtattgaaa atgccgacgc
     4141 aaaagaactt ttaatgcagc ttgccgagca ataccaacgc gaggctgaca cagaagacac
     4201 aagcctcgcg aacatcagtg cggctcttaa gctcgcaaaa atatatgccc gtgccggtat
     4261 ggcagacgac gctctgcgtt cactcgaggg actacgcgag gcggcatttc acgagcgtga
     4321 ggcaagcaat gtgcgtttta ccgaaatttc aagagttata gacgacatcg aaagtggagg
     4381 tgtgtggaag gcataggtca ttatattttt tacatgtaat accttgctca cttttgcaat
     4441 tagcttaaag tttcaaatct ggcggggacg atgcttggaa ctttgaaccg acttgtcctg
     4501 agcgcagtcg aaggaaaacg tggctcaatt tcgaggatgt caataaaagt atacagattt
     4561 gaaacgctgg gagagctgac tgctgaactg tacaggtcga tatattatta caacaactta
     4621 cgcattcaca cgagtctggg aatggcgcca aagaaattca cagaaaaatt tgcgcaagag
     4681 aaagaaatca aat
//
LOCUS       DATDSU010000025        13857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1860008, whole genome shotgun sequence.
ACCESSION   DATDSU010000025 DATDSU010000000
VERSION     DATDSU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860008"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VI981_00720"
     CDS             complement(<1..657)
                     /locus_tag="VI981_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY4478867.1"
                     /translation="MKRLKRGFTWLVEDGTAPSVFSLLRLKTLKLKLSYFFQLIRNLC
                     RKQVPSFTTGFTLIEIMIVIAIFAVLMTIIVANIQSARLKSRDDGRISDIQQIQLAIE
                     RYYEKNRYYPGSLISLVSSGLLPSVPKPSAGAGQTDYTYASFLATGSFPECTGYHLGA
                     VLESENKILNQDGDADSPARTTSLCNGATPEFDGKTVACSGTSSVPSGERCYDVIEQI
                     S"
     gene            complement(673..1194)
                     /locus_tag="VI981_00725"
     CDS             complement(673..1194)
                     /locus_tag="VI981_00725"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY4478868.1"
                     /translation="MRILKKSQKGFTVIELLIVVSIIGLLSSIVMASLNSARIKGADA
                     AIKTDLSGIRSSAAVEFENLNSSYNNTGSTIILTSGASNCDGATTANSILANTSIQRA
                     VPHANAQAKKPFEYPICVIASSAYAIASPTRVGSTGNFWCVDSNGEGKAITVGTVANV
                     VDTDTSCALLDLQ"
     gene            complement(1335..1832)
                     /locus_tag="VI981_00730"
     CDS             complement(1335..1832)
                     /locus_tag="VI981_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4478869.1"
                     /translation="MVIHNKSQGFTLIELLVVIAILAVLSAVVMAALNSARSKGANAA
                     IKSNLSGIRSHAELQNTTYNCYVGIAGDCSFLASGNVACSNAAVTTEHIFRKPEILSA
                     ITAAGNAYNNGGLASATCNQIANGGAWAVSVPLKVAEGTITTWCVDSTAASKGRTGVI
                     LATNC"
     gene            complement(1967..2455)
                     /locus_tag="VI981_00735"
     CDS             complement(1967..2455)
                     /locus_tag="VI981_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4478870.1"
                     /translation="MFKNKNNNNKGFTLIELLVVIAIIGILSSVVLASLNTARGKGAD
                     ASIKANLSGVRAAAELYYDGTSNYDTESSVCTAQLFDEVASIKSAIAAVEAQNTGNVV
                     CRSSDGTATAAQVASSWAISSQLKTGTTDFWCVDSAGASKQTAANITASADTVATLAV
                     CP"
     gene            complement(2759..2980)
                     /locus_tag="VI981_00740"
     CDS             complement(2759..2980)
                     /locus_tag="VI981_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478871.1"
                     /translation="MKKMTIDGLARMIEENVIGNMATKDGLKDGLKAVTERLDTIDDR
                     LTKIENNHERRITNLEDDVIRIKAKVAIK"
     gene            complement(3155..3397)
                     /locus_tag="VI981_00745"
     CDS             complement(3155..3397)
                     /locus_tag="VI981_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478872.1"
                     /translation="MARMTIEGLAKMINENVVKKMATKVDMDARFEQVNERFERVDNR
                     LEKVEDRLVKIENGHVGRIEKLEDDVRVVKTKIGIR"
     gene            complement(3554..3778)
                     /locus_tag="VI981_00750"
     CDS             complement(3554..3778)
                     /locus_tag="VI981_00750"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEY4478873.1"
                     /translation="MKIAYDKKVDSLNVTLRIGKVEKTIEVAPEVFLDVDKNGNPLYL
                     EIVGVRAKIGSDFSKITIGKKSVPVSLLAM"
     gene            complement(3798..4025)
                     /locus_tag="VI981_00755"
     CDS             complement(3798..4025)
                     /locus_tag="VI981_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEY4478874.1"
                     /translation="MKIVFSTHALTKLKQRNIDKRLVLDTIRAPQFKRPAHQFREERI
                     KYFGRNWLRVVVVKEGDTFVVVTAHWIDRLR"
     gene            complement(4027..5235)
                     /locus_tag="VI981_00760"
     CDS             complement(4027..5235)
                     /locus_tag="VI981_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4478875.1"
                     /translation="MLFNYKSIDTQGVAREGSIDAINIDVAINSLQRRGLVVSSVAPV
                     DEDKNLLSKQLSFFTRISNKDVVILSRQLATLFEAQVSALRIFRLLMAESSNQKMSNV
                     LKAVADDLQGGSSISKALSRHPKVFSNFYVNMVRSGEESGKLDQIFIYLADHLDRTYE
                     VTSKARNALIYPAFVVVTFIVVIILMFTMVIPNISVILKESGQDIPIYTTAIIAISDI
                     LIRYGYILLVMVIVFIFFTLRYIKTPAGAMTADRFKLSVPILGKLFEKLYLSRIADNM
                     NTMLISAIPIVKALEITSEVVGSPVYREVINDVKEKVKTGSPLSESLGKHLEIPGIMS
                     QMVKVGEETGEMGQILKNLAKFYRREVTNAIDTLVGLIEPAMIVFLGAGVGILLAAVL
                     VPIYSISSAL"
     gene            complement(5308..5694)
                     /locus_tag="VI981_00765"
     CDS             complement(5308..5694)
                     /locus_tag="VI981_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY4478876.1"
                     /translation="MRNTKQIPISNEEEKPKYDLEERTFLFAQNVRSFVKTVPRDISN
                     VEDTKQLVRSSGSIGANYIEANECLGKKDFLMKIRIARKESKESIFWLRLIDTKNDES
                     RKIRDHLLNESKELMLILSAIMRKSQ"
     gene            complement(5708..6766)
                     /locus_tag="VI981_00770"
     CDS             complement(5708..6766)
                     /locus_tag="VI981_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY4478877.1"
                     /translation="MDYKTELDDLIGTVSREGASDLHISEGRHPMIRVSGFLVPLLKK
                     PVLTKADTMALSKELIPKSKQELFYSKKEIDFSYTFQDVRFRCNAYVQQGAVGLALRL
                     IPKQIRTLEELNLPPILEQFTKKPQGFFLVVGPVGHGKSTTLSSMINIINEERAEHII
                     TIEDPIEFIYEQKRSIIDQREVGNDTDSFEIALKSIFRQDADVLLIGEMRSPDTISTA
                     VTAAETGHLVFSTLHTNDAAQTINRIIDSLSSDQQDQIRVALASSLTGIFSQRLISRI
                     SGGLIPAYELLINTSAVSNLIREKRVHEINTVIETGLEQGMIDMNRSLADLVRAGEIT
                     VESAFMHSLNAKVLERMI"
     gene            complement(6999..7394)
                     /locus_tag="VI981_00775"
     CDS             complement(6999..7394)
                     /locus_tag="VI981_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4478878.1"
                     /translation="MPIGEKKKIYIVDDDEVLLNMYTIKFRSAGFDVEVSKDSLYALE
                     KLRAGLTPDVLLLDVVMPGTDGIELLENIRKEGLVSNAVVLILSNQSAPEDIERAKNL
                     KVDGFIVKATTIPSEVVIEVQNAMKAHAK"
     gene            complement(7490..9265)
                     /locus_tag="VI981_00780"
     CDS             complement(7490..9265)
                     /locus_tag="VI981_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY4478879.1"
                     /translation="MTLLDLLIEKKLIDPETLPEVKEAIRHSGGNIDEALAAFNINPA
                     DLLATRGEYYGIPIRNLEGQDVPFEILKYVPEESALHYKFVPIGLEEGILEVGIIDPD
                     NIEARDALNFISSKINMPYKVYLIAPADFERISHTYRGMSGEVTEALSEIENELDDAI
                     NAADSGGGESEKPKIDGAVGMDGIKSKDERKSGRTTKIIEDAPVTKIVATMLNYATQG
                     NASDIHVEHMEDKVRVRFRVDGVLNTSLILPTKIHAAIVARIKILCNMRLDEKRKPQD
                     GRFSAKIEGRKVDFRVSTFPAYYGEKIVMRILDSERGVKNLVDMGMTERNLQAVRRAL
                     DKPYGLILISGPTGSGKTTTLYSMLKEVDKETQNVISLEDPVEYNIPSMNQSQIKAEI
                     GYTFANGLRSVLRQDPDIIMVGEIRDKETAQLAVQAALTGHLVFSTIHTNNAVGVIPR
                     LIDMGVDPFLIAPTLVLAMAQRLTLTLCPNSGKPLAVDQSIKMLVDKEFADVPIQFRT
                     ELPDASTVYEAEVSPQCPKGTMGRTAVFEVLEMSKDLEAVILKNPTDIEIMKVARIQG
                     MLTMREDAIIKAFKREIPFSEVNKL"
     gene            complement(9480..9824)
                     /locus_tag="VI981_00785"
     CDS             complement(9480..9824)
                     /locus_tag="VI981_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478880.1"
                     /translation="MNHNLKTILILVAILAIAGLGYKFLIVNNRIPSIAAVSTTDVTD
                     QTAREVVTMLEKLRTLKLDKSIFTNEMFLSLEDFNVQVIDEERGRQNPFMPFEPEPTI
                     SKSKPPSVLRRE"
     gene            complement(9962..10489)
                     /gene="pilO"
                     /locus_tag="VI981_00790"
     CDS             complement(9962..10489)
                     /gene="pilO"
                     /locus_tag="VI981_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY4478881.1"
                     /translation="MIRLTPIILILMSIGLFFLVIDPTFKKLQAIQVDASRYANVLDQ
                     AKQLERIRDNLLNEYNTIDEASREKLKKLLPDEVDTVRLVMDINDIARQHSLIVRGLN
                     LATDNKAKKNTPAGELQEIGFGFGVEASYDEFISFMKALERSLRIIDVVSINITAGKD
                     DRYNFAVNAKIYWMK"
     gene            complement(10493..11176)
                     /locus_tag="VI981_00795"
     CDS             complement(10493..11176)
                     /locus_tag="VI981_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478882.1"
                     /translation="MDTKFQPSFIPKKPMGTRDFGNAIRNSKIGLFTLLGMVIFIITL
                     GLTGFVYLYKAYTEKDIQKMKIRIEETRAKFQPETIQDLVRTHERVKMAKQILDRHIS
                     TTALLQVLADNTYQNVRYKTFSYAFGSDNAISLNMTGEAKSFNSVALQSDRFRAHKLF
                     SSPIFSGLTLNEKSYVEFNFTSEVDPLIALYKNAFEAVSSESESDVPNVSEEDAVPSG
                     EGAASDSSL"
     gene            complement(11662..12789)
                     /gene="pilM"
                     /locus_tag="VI981_00800"
     CDS             complement(11662..12789)
                     /gene="pilM"
                     /locus_tag="VI981_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY4478883.1"
                     /translation="MMKFENPIKKFFTKKDDSVLGVDIGPSYIKVVQLKRKHGKAILE
                     TYGALALGPYAGIEIGKATALPPEKLSEALIDLMREANVTSRNCGVSIPMSSSLVTNI
                     EVPHVEAKQMAQIIPIEARKYIPVPITEVLLDWWEIPREESSPNEFEMDEKEEKKKSE
                     KSEVLLVVIHNDALARMQEVIKLSELEATFYEIEIFSTIRSVMDREGTPIMIFDLGAS
                     STKLYIVDKGILRTSHIINRGSQDLTMAISKSMSISTEEAENLKRSTGFTPVAEGTDI
                     ISVVSSTLSFIFSEAERVLLNYQKKYLKNIGKIVLTGGGVGLKGFKEIAQETFGVNVV
                     FADPFAKVESPAFLEGVLKQVGPEFAVSVGLALRKLQENDK"
     gene            12881..13546
                     /locus_tag="VI981_00805"
     CDS             12881..13546
                     /locus_tag="VI981_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY4478884.1"
                     /translation="MDFKGILERISSLASSVSDWLFPIHEDISNFLNSNESLVGAYAI
                     SQNGANEFEAILNYRNRVVRRAVWELKYRGNRAVARKFAQLLEDRLQSVLEDEVLFEN
                     TKALVIPIPLSEARRRERGFNQMELVLEAVPRGDKNYFEVANGALIKIRDTPPQTSVR
                     KRKDRLSNLNGCFTVVDKNSVKNKTVVLVDDVTTTGATLSEATRELKKSGAKKVFAIA
                     LAH"
     gene            complement(13646..>13857)
                     /locus_tag="VI981_00810"
     CDS             complement(13646..>13857)
                     /locus_tag="VI981_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478885.1"
                     /translation="GFQLHAPKLIRFGELTEDEYFCSEDGAKQGVTFRNMSDTEPLVT
                     LRYFGPEVNPDAPKMGAYRKNDDLD"
BASE COUNT         3726 a         3145 c         2899 g         4087 t
ORIGIN      
        1 ggagatttgt tcgatcacgt cataacaacg ctcgcctgac gggacagagc ttgtacccga
       61 acacgccaca gtcttgccat caaattcagg tgtcgcccca ttgcaaagtg aagtagttct
      121 cgcgggagag tccgcatcgc cgtcttgatt aagaattttg ttttcactct cgagtacggc
      181 gccgaggtga tagcccgtac attcaggaaa actcccagtc gctaagaatg aagcgtaagt
      241 ataatctgtc tggccagcac cagccgaggg cttaggaact gaagggagta gccccgagct
      301 tacgagtgaa atgagcgagc caggataata acggtttttt tcatagtacc gctcgattgc
      361 cagttgaatt tgttgaatat ctgatattcg tccatcatcg cgcgacttta gacgagcaga
      421 ctgaatattg gccacaatga tagtcatcaa aactgcaaat atagcaatga caatcattat
      481 ttcaattagt gtaaacccgg tagtgaaaga tggcacttgt tttctgcaca aatttctaat
      541 taactgaaag aaataagaaa gcttcaattt taaagttttg aggcgtaaaa gagaaaaaac
      601 acttggtgca gtgccatctt ctactaacca ggtaaagcct ctctttaatc tcttcatgta
      661 gttattcgtt gtttactgca aatcgagcag tgcgcaggat gtatctgtgt caacaacatt
      721 cgcaaccgta cctaccgtaa tagccttccc ctcgccgttg ctatctacac accaaaagtt
      781 tccggtgctc cccactcgag tcggcgaagc gattgcatac gccgaagaag caatgacgca
      841 aattggatat tcaaagggct tttttgcttg agcgttagca tgtggtactg cacgctgaat
      901 gctcgtattt gccaaaatcg agttcgccgt cgtcgccccg tcacagttgc ttgctccact
      961 ggtgagtata atagttgaac cagtattgtt atagctggaa ttcaagtttt caaactcaac
     1021 agccgcactt gatcgaatgc cagacaagtc agtcttgatt gccgcgtcgg ctcccttaat
     1081 cctcgcagaa tttagagacg ccataacaat agacgacaga agcccgataa tagacactac
     1141 tatcaaaagt tctattacgg taaatccttt ttggcttttt tttaaaatgc gcatcttgga
     1201 gattaagtta aagcgttcac actttaattt tatgcgaatc gattgtaaat agtataacac
     1261 cgcgccaaca aaaaccatgt ttacaacaga aactcccgtc cgggagtttt ttgtaagtac
     1321 acaattgcga aaacttaaca gtttgttgcc agaattactc cggttcttcc ctttgaagca
     1381 gctgtgctgt caacacacca tgtggtgatc gttccttcgg caactttgag tggaaccgat
     1441 accgcccaag cacctccgtt tgcgatctga ttgcacgtag cgctcgccaa ccccccattg
     1501 ttgtaagcgt ttcctgcagc agtgatcgct gaaagaatct ctggtttccg gaatatatgc
     1561 tctgtagtaa cagcggcatt gctacaggca acgttgcctg aagcaagaaa tgaacagtct
     1621 cctgcaattc caacataaca gttatatgta gtgttctgaa gttccgcgtg agaacgaatg
     1681 ccgcttaagt ttgattttat ggctgcattt gcccccttac tcctggcgga attcagggca
     1741 gccattacaa cagcagagag tactgccagt attgctataa ctacaaggag ttcaataaga
     1801 gtgaatcctt gcgatttatt atgtatgacc attttaacaa aaatatccgg ccttaacctt
     1861 aatggattat tataacattt atcagaaatg tctatgttga taaataagac ataaaacaaa
     1921 agctcctaaa ggaaaggagc ttttgcaaat gccaattcaa tcaaaattac ggacacacag
     1981 ccaatgttgc aaccgtatcg gccgaggcgg taatattcgc ggcggtttgt ttcgaggcgc
     2041 ctgcactgtc tacacaccaa aaatcggtag tacctgtttt taattgacta gaaatagccc
     2101 acgaagaggc gacttgggct gcggtagccg ttccgtcaga tgaacgacat acgacgtttc
     2161 cagtattttg agcctcaact gctgcaattg cagatttaat cgaagcaacc tcatcgaaaa
     2221 gctgggcagt acacacagac gactcagtgt cataattgct ggtaccgtca tagtagagtt
     2281 cagcagctgc cctcacacct gacaagttgg ccttgattga agcgtctgcc ccctttcctc
     2341 gtgccgtgtt tagagatgcg agaacaacag aagaaagaat accgatgatg gcgataacta
     2401 ccagaagctc aatcaacgta aaacctttat tattattatt tttattttta aacatgttta
     2461 aacttttaat gaatttgatg gcttcatgca tgaggagcca tcttgattaa atcgtttccg
     2521 ccgtaggcga tccgcctgtg ggcggaagat tatttcaggg attatttcga ccaccccaaa
     2581 acaactaaac cgaatggagt aattatagca ggaacagccg taatggcatg tctcgactgt
     2641 ggatatccag aaacagctta taactgcttg tttttagctt atagttagaa aaaagctaga
     2701 tgaggatgga aaattgccat acccaaggtt cgaccttcac aaactgaaat aagtacacct
     2761 actttatcgc aacttttgct tttattctga taacgtcgtc ttccagatta gtgattctgc
     2821 gttcgtgatt gttttctatt ttcgtgaggc gatcgtcaat cgtgtctagt cgttccgtta
     2881 ctgctttgag accatctttg agaccgtctt ttgttgccat atttccaatt acgttctctt
     2941 caatcatgcg agccagaccg tctattgtca tttttttcat aggatcatta ctttaagtat
     3001 atccactgat tgaaagtggt ttttctttct catatgacca atgatactaa attgaataat
     3061 ttttgtatat tgcacttcat ccgcgttcaa ggtagaacct tttatcgaat taaggttcga
     3121 cctttttaga gatttaaggt cggaccttga ttacttagcg aatgccaatc ttggttttta
     3181 ctaccctcac gtcatcttct agtttttcaa ttcggcccac gtgaccattt tcaattttga
     3241 ccagtctatc ctcaactttt tccaacctgt tgtccaccct ctcaaatcgt tcgtttactt
     3301 gctcaaagcg ggcatccata tcaactttag ttgccatttt tttgacaaca ttttcattga
     3361 tcatcttagc tagcccctca atagtcattc gcgccatatg ttaaaattat acccaaacaa
     3421 attttattgt atatatcgaa actctaccaa aactcaaggt cgaacctttt atcgattccc
     3481 gccttcgcca aggctacgga caggcaggta aggtccgacc tttttagaga ctaaaagtca
     3541 gaccttgggt tatctacatg gccaataggg acacgggtac agattttttt ccgatggtaa
     3601 ttttggaaaa atcgctacca attttagctc gaacaccgac aatttctaaa tacaagggat
     3661 tgccattttt gtccacatct aaaaaaactt caggagcaac ttcaattgtc ttttcaacct
     3721 ttccgatcct taaggtaaca tttagcgagt ctactttttt atcatacgct atcttcataa
     3781 tggtaactgt accagaatta gcgaagtcga tcaatccagt gagctgttac gactacaaaa
     3841 gtatctcctt ctttgaccac aacaaccctt agccagttcc tgccaaagta cttgatccgc
     3901 tcctcccgaa attgatgcgc tggtctttta aactgcggcg ctctaatagt atcgagcact
     3961 agtcgtttat cgatatttcg ttgtttgagt tttgttagag catgggtgct aaaaacaatt
     4021 ttcatgttac agtgccgagg aaatactata gataggaaca aggacggcgg cgagaagaat
     4081 tccgacgccg gcacctagaa acacgatcat tgccggttca atcaggccaa ccagagtgtc
     4141 gatggcgttc gttacttcgc gtctgtaaaa ttttgccagg ttctttagaa tttgccccat
     4201 ttctccggtc tcctctccca ccttgaccat ctgactcata attcccggaa tttcaaggtg
     4261 tttccccaaa gattccgaga gaggacttcc cgttttaacc ttctccttta catcattaat
     4321 cacctcacga tacacggggc ttccgaccac ctcggacgtg atctcaagcg ccttcacgat
     4381 cggaattgca gaaataagca tcgtattcat gttatcggca atacgcgaca gatacagttt
     4441 ctcgaagagt tttccgagaa ttgggacgga gagcttgaag cgatcggcgg tcatggctcc
     4501 tgccggcgtt tttatatacc gaagcgtgaa gaaaataaaa acgatgacca ttaccaagag
     4561 gatatatcca tatcgaatca ggatatcgct tatggcgata atagccgtcg tataaatagg
     4621 aatatcctgc ccagattcct tgagaataac gctgatattc ggaattacca tcgtgaacat
     4681 gaggatgata actacaataa aagtaacgac cacgaacgcc ggataaatga gggcattgcg
     4741 ggcttttgat gtaacttcgt aggttcggtc aaggtggtca gcaagataaa tgaaaatctg
     4801 atcgagtttg ccggactctt ctccagatcg caccatgttt acgtagaaat ttgaaaaaac
     4861 cttcgggtgt cttgagagcg ctttcgatat tgaacttcct ccctgaaggt cgtctgccac
     4921 cgccttcagg acattggaca tcttctgatt tgaggactct gccatgagaa gcctgaagat
     4981 acgcagtgcc gagacttggg cctcaaaaag agtcgctagc tgccgagaga ggataacaac
     5041 atccttgttg ctgatgcgag tgaaaaatga gagctgtttt gaaagaagat tcttgtcttc
     5101 atcaacggga gcaaccgacg acacaacaag ccctcttcgc tgcaacgagt tgatcgcaac
     5161 gtcaatattt atggcatcga tggatccttc ccgcgcgacc ccctgcgtgt cgatagattt
     5221 gtagttgaag agcatacttt taaaaattcg aaattcgaat atcgaaattc gaaacaatat
     5281 ctaaattata aactaaaaat aggaaaatca ttgtgacttt cgcataatag cacttaaaat
     5341 aagcattaat tccttagatt cgttaagtaa atgatctcta atttttcgtg attcatcgtt
     5401 ttttgtatct atcaatctca accaaaaaat actctctttg gactcctttc tggcaatacg
     5461 tattttcata agaaaatctt tttttcccag acattcattt gcctcaatat aatttgcacc
     5521 aatagaaccc gaggagcgaa caagctgttt agtatcttct acattagaaa tatcccgcgg
     5581 cactgtttta acgaagcttc ttacattttg tgcaaagaga aacgttcttt cttcaagatc
     5641 gtatttgggt ttttcttcct cattagatat tgggatttgt ttcgtatttc gtatttcgtg
     5701 cttgaggtca tatcattctt tcaagcacct tggcgttcag tgaatgcatg aaggcacttt
     5761 caaccgttat ttctcccgca cgcaccaagt ctgcgagcga tcgattcata tctatcattc
     5821 cttgttcaag tcctgtctct atcacagtat tgatttcgtg aacacgcttt tcacgtatga
     5881 ggtttgagac agcgctcgtg tttataagga gttcatacgc gggtataaga cctccggaga
     5941 tacgcgagat tagtcgttga gaaaaaatac cggtgaggct tgaggcgagc gcgacacgga
     6001 tctgatcttg ctgatccgac gacaatgaat caataatacg gttgatcgtc tgcgccgcat
     6061 cattcgtgtg tagtgttgaa aagacaaggt gacctgtttc cgcggccgtg actgcagtcg
     6121 aaatcgtatc aggactgcgc atttcaccga tgagaagaac atcagcgtct tgtcggaaaa
     6181 tacttttgag cgctatttcg aaggaatcag tgtcatttcc aacttcccgc tgatcgatga
     6241 tagaccgttt ttgttcgtaa ataaattcaa tagggtcttc aatcgtgata atgtgctcgg
     6301 cgcgctcttc gttgattatg tttatcattg aagaaagcgt ggtgctcttg ccgtggccca
     6361 caggtcccac aaccaaaaag aagccttgcg gttttttcgt gaactgctcg agaatggggg
     6421 gcaaattgag ctcttcaagc gttctgattt gcttcggaat cagacgaagt gcgagaccga
     6481 cggctccctg ctgaacatag gcgttacagc gaaaacgaac atcctggaac gtatacgaaa
     6541 agtcgatctc cttcttagaa taaaagagtt cctgtttgct ctttgggata agctcctttg
     6601 agagcgccat cgtatccgcc ttggtcaaga caggtttctt taagagcggc actaaaaagc
     6661 cagagacacg gatcatcgga tggcgaccct ctgaaatatg cagatcagat gccccttcac
     6721 gcgacactgt tcctatcaaa tcatctaatt ctgttttgta gtccatgggg tagtgaaaaa
     6781 tggcattgct cttttggagc aattatacta tttttttcta aaattactga taacgctgca
     6841 aatcttcatt aatttaaaaa acttttacct ctaatgccat tttctactac cccatccact
     6901 gtattgaatt atcaattatc aatttacaag tatcaattaa tcggaatctt gtttgaaaat
     6961 tattcttact ttggtcattg acttttgaaa attgaaaatt acttcgcgtg tgccttcata
     7021 gcattttgaa cttctataac aacctctgat gggatagtgg tagcttttac gataaatcca
     7081 tctaccttca gattttttgc ccgttcaata tcctctggag cgctttgatt cgataaaata
     7141 aggaccacgg cgttcgacac caagccctct ttgcgtatat tttcaagaag ttcgatgcca
     7201 tcagtgcccg gcataaccac atcaaggagc aatacatcgg gtgtcagtcc tgctcgaagt
     7261 ttttcgagcg cataaagact atcttttgac acctcaacgt caaaaccggc actccggaat
     7321 ttgatcgtat acatgttcaa aagcacttca tcgtcgtcga caatataaat ttttttcttt
     7381 tcgccaatag gcataatact aatatactaa ttgataccaa tatacgaatg gatacgaata
     7441 ttctgcatta gtattatttg tatattagta ctcattcgta tattggtatt tacaatttat
     7501 tgacttcgga aaaaggaatc tcccgcttga acgcttttat gatagcatct tccctcatgg
     7561 tgagcatccc ctgaatccgc gccactttca taatttcgat atcggttggg tttttcaaaa
     7621 taaccgcttc caaatctttg gacatttcaa gtacttcaaa aactgcggtt ctgcccattg
     7681 ttccctttgg acactggggg gagacttcgg cttcatatac ggtagatgcg tcaggcagtt
     7741 ctgttctgaa ttgtataggc acgtctgcaa attctttgtc aaccagcatc ttaatgctct
     7801 gatccacggc gagcggcttt cctgaattag gacagagcgt aagcgtgaga cgctgggcca
     7861 tggcaagaac gagcgtggga gcgataagaa aagggtcgac tcccatatcg atgagacgcg
     7921 ggataacgcc gaccgcgttg ttggtgtgaa tagttgaaaa cacaaggtgt ccggtgagcg
     7981 ccgcctgaac agcgagctgt gcggtttctt tatcgcggat ttcaccgacc atgataatgt
     8041 ccggatcctg ccggagtaca gaacgtaaac cgtttgcgaa agtgtagcca atttctgcct
     8101 tgatttgcga ctggttcatt gacggaatat tgtactcaac ggggtcctca agcgaaataa
     8161 cgttctgcgt ctctttgtct acctccttca acatcgaata cagtgtcgtt gtctttccgg
     8221 aacctgttgg gccggagatg agaatgagcc catatggttt atcgagagct cttctcacgg
     8281 cctgtaagtt tcgttccgtc atgcccatat ctacgagatt tttaactcca cgctccgaat
     8341 cgagaattcg catgacgatt ttctctccgt aatacgcagg aaatgtcgag acacgaaaat
     8401 ccacctttcg cccttcgatt tttgccgaaa aacgaccgtc ttgaggtttg cgcttttcgt
     8461 cgagacgcat attgcagaga atttttattc gcgccactat ggctgcatga attttagtcg
     8521 gcaaaataag actagtgttc aacaccccat caacacggaa tcttacgcgt actttgtctt
     8581 ccatatgctc gacgtggatg tcagacgcat tcccttgcgt cgcgtagttc agcattgttg
     8641 ccacaatctt tgtcacagga gcgtcttcaa tgatttttgt cgtcctgcct gattttcgct
     8701 cgtcttttga ctttataccg tccattccaa ccgcgccatc aattttaggc ttttcgcttt
     8761 cacctccacc tgaatcggct gcgtttattg cgtcgtcgag ttcattctcg atctccgaga
     8821 gtgcttcagt tacttccccc gacattcctc tgtaggtatg cgaaatacgc tcgaaatccg
     8881 caggcgcaat gagatatacc ttataaggca tgttaatctt gcttgatatg aaattcagtg
     8941 catcgcgtgc ctcaatattg tctggatcta tgattcccac ttccaaaatt ccttcctcca
     9001 gtccaatggg gacaaattta tagtgaagcg cagactcttc cggaacatac tttaatattt
     9061 cgaatggcac atcctggcct tcgagatttc tgattggtat tccgtaatat tccccacggg
     9121 tagccagaag atctgcaggg ttaatattga aagctgcaag cgcttcgtcg atatttccgc
     9181 cagaatggcg gatagcttcc tttacttcag ggagcgtctc gggatcaata agtttttttt
     9241 cgatgagaag gtcaagaagg gtcatgccaa tttattatta acgcagtgaa ttaataaatt
     9301 gagcacccca tgcaatacga tttgaaagtc tactttttgg gttcgatcct tttggatagt
     9361 ggtttaatgt ggtcatcaat tttatacttt ttttcttcaa atcgttcttc agattctgaa
     9421 tctgaatctg aagatattgc acggggtaaa gaatgtatat tcgctacgct cattacattc
     9481 tactcccttc ttagcaccga cggtggcttc gacttagata tcgtgggttc aggttcaaag
     9541 ggcataaagg ggttctgcct tccgcgctct tcatctataa cttgaacatt gaaatcttcg
     9601 agcgaaagaa acatttcatt cgtgaaaatc gatttgtcca attttagcgt ccgaagtttc
     9661 tcaagcatcg ttacaacctc gcgggcagtt tggtctgtca cgtcagtcgt actgactgcc
     9721 gctatagagg gaattctgtt gttcacgatc aaaaatttgt agccaagccc tgcgattgca
     9781 agtattgcca caagaatgag tatggttttg aggttgtgat tcatggggta gtgaaaaatg
     9841 gcatcgctct atggagcttt agtaagctaa attttaacaa aattgatagt cgtaaacttg
     9901 ttgtcatatc ttttttaaag tcatatattc tctaatgcca ttttctacta ccccattcat
     9961 gctatttcat ccaatatatt tttgcattaa cggcaaagtt atatctgtca tctttacctg
    10021 cggtaatgtt aatactcacc acatcgataa tgcgcaaact ccgctcaagc gccttcataa
    10081 aactgataaa ctcatcatat gaggcttcta ccccaaagcc aaaaccgatt tcctgtagct
    10141 cacccgcggg tgtgttcttt ttagctttat tatcggttgc caagttaagg ccacgcacga
    10201 tcagactgtg ttgcctcgca atatcgttta tatccatgac caatcgcacc gtgtctactt
    10261 catcaggcag taattttttc agtttttcac ggctcgcctc gtctattgtg ttgtactcat
    10321 tgaggaggtt atcacggatt cgttcaagct gtttggcctg atcaagtaca tttgcatagc
    10381 ggcttgcgtc gacttgaata gcctgaagct ttttgaacgt cggatcaatg accaagaaaa
    10441 agaggccgat cgacataagt attaagatga tgggagtaag tcgtatcatg gtttacaaag
    10501 agctatcact agccgctcct tctccgctcg gtaccgcatc ttcctcactc acgttcggca
    10561 catcactctc ggactcggaa gatacagctt caaaagcgtt tttgtagagc gcgatcaacg
    10621 gatcgacctc tgatgtaaag ttaaactcga cgtatgactt ttcgtttaaa gttaaacctg
    10681 aaaatatggg gctgctaaaa agtttatggg cgcgaaaccg atcagactga agcgcaaccg
    10741 aattgaaact ttttgcttcc cctgtcatat tgagagagat cgcattatcc gacccaaaag
    10801 cgtacgaaaa tgttttgtat cgaacgttct ggtatgtatt atcagccagt acctgaagta
    10861 atgcggtcgt cgaaatatgt cggtccaaaa tttgtttggc catttttacc cgctcatggg
    10921 tacggaccag atcttggatc gtctccggct gaaattttgc tctagtttcc tcgatccgta
    10981 ttttcatctt ttgaatatcc ttctccgtat aggctttgta gagatagaca aaaccggtaa
    11041 gaccgagcgt gatgataaaa atcaccattc caagcaacgt aaaaagacca atcttggaat
    11101 tgcgaattgc atttccgaaa tcgcgtgtcc ccatcggttt tttgggaatg aaagatggtt
    11161 gaaacttggt gtccattccg taagaactaa ttaaaaaagc actaaagtcg agtcaactca
    11221 ttatagcgtg agccccgtat aagacataga gggtgttgtg tgtataaata gagagccctt
    11281 gaatcggtcg tcttatttcg gccagcgaga tgagaagctc cgggggagct tctcaagacc
    11341 ttttgagctc gagagtttga gagccaaaag gtgtacagct tctgtaagaa gcgtgtggaa
    11401 aattctgatt ttatacagcg tccgacctgc ccgccaatgc tagagcgata ttcgtggtaa
    11461 tttgaggtaa agtgacatat ttacaagttt acattttatt taaccccata ctgtgcattg
    11521 ggcgatggca ggcggggcga agacgaatca aaggtcaata ctttgtggct gacttcggct
    11581 gagctcagtc gaagccctgc cacgcatcag tgcgagcgag gcgagttcct ttaacactct
    11641 ggggctttgc cccaaggacc tttatttatc gttctcctga agtttccgaa gtgcgagccc
    11701 caccgatacg gcgaattcgg gaccaacttg tttcaatact ccctcaagaa aagccgggga
    11761 ttcgaccttg gcaaacggat cggcaaaaac cacgttcacg ccgaaggttt cctgtgctat
    11821 ctctttaaaa cctttgagcc ccaccccgcc gccggtcaac acgattttcc ctatgttctt
    11881 tagatatttt ttttgatagt tgagcaggac acgttcagcc tccgaaaaaa tgaaagacag
    11941 tgtcgatgaa acgacagaaa taatgtctgt accttcggcc acgggcgtaa agccggtcga
    12001 gcgctttaaa ttttctgctt cttcggtact gatactcata gattttgaaa tagccatagt
    12061 caaatcctgc gagccgcggt tgatgatatg cgacgttcgt aaaattcctt tgtcgacaat
    12121 atagagtttc gtactgcttg cgcccaaatc aaaaatcatg atcggcgtac cttcccgatc
    12181 catgacagag cgaatcgtac tgaaaatttc aatttcataa aatgttgctt caagctctga
    12241 aagcttgatc acctcctgca tccgtgcaag cgcatcgttg tgaatcacca ccaaaagcac
    12301 ttcggatttt tccgattttt tcttttcctc tttttcatcc atttcaaatt cgttcggact
    12361 ggattcttct ctgggaattt cccaccaatc aagcaagact tcggtaatcg gcacggggat
    12421 atattttcgc gcctcaatgg ggattatctg cgccatttgc tttgcctcca cgtgcggcac
    12481 ttcaatattg gtaacgagac tcgaagacat aggaatcgaa acgccgcaat tgcggctcgt
    12541 tacattggcc tcgcgcatga ggtcgatgag cgcttcggaa agtttctcgg gcggcagcgc
    12601 tgtggctttt ccgatttcaa tgccggcata cgggccgagc gccaacgctc cataggtttc
    12661 aagtatcgcc ttgccgtgct ttctcttaag ttggacaact ttaatgtaag aaggtccgat
    12721 atcaacgcca agcacagagt cgtctttttt cgtaaaaaat ttttttattg ggttttcgaa
    12781 cttcatcatc cgttgcgtct aggttcgtgt gatctacaat gattaagccg cacgagagtc
    12841 tctcgtgcac gctttgattg taattgtagc attaacgcat atggatttta aaggtatttt
    12901 agagcgcatt tcttcacttg cctcaagtgt gagcgactgg ctctttccaa ttcatgaaga
    12961 tatttcgaat ttcttgaact cgaatgaatc gcttgtgggt gcctatgcga tttcgcaaaa
    13021 tggagcgaat gaatttgaag ccattctaaa ttatcgcaac cgtgtcgtcc gaagagcggt
    13081 ttgggaatta aaatacagag gcaatcgtgc cgttgcgcga aaattcgcac agttacttga
    13141 agatcgtctg caaagcgtac tcgaagatga ggttcttttt gaaaatacca aagctcttgt
    13201 gattcccatt cccctatccg aagcgcgcag gcgcgaacgc ggcttcaatc aaatggaact
    13261 tgtacttgaa gcagtgcctc gaggtgataa aaattatttt gaagtcgcaa acggcgcgct
    13321 cataaagatc agagacacgc cgccgcaaac ttcagtcagg aaacgaaaag acagactttc
    13381 aaatctcaac ggatgtttta ctgttgtaga caaaaatagt gtcaaaaaca aaaccgttgt
    13441 acttgttgac gacgtgacca caacaggcgc gactctttcc gaagctacgc gcgaattaaa
    13501 aaaaagcggc gccaagaaag tatttgctat tgcactggcg cattaacgcc ctcccgtacc
    13561 aatacttgtt cgcatttttt acttttttac ataaaaaacc actgaaaata cagtggcttg
    13621 atctcacgat tgacgttcaa ccgcgtcagt cgaggtcgtc atttttccga taggcgccca
    13681 ttttcggcgc atccgggttc acttcaggac caaaatagcg gagcgtgact agcggctctg
    13741 tgtcgctcat gttacgaaag gtcacccctt gtttcgcacc atcctccgaa cagaaatact
    13801 cgtcttcagt cagttcgcca aaacgaatga gtttcggtgc atgaagctgg aaaccgt
//
LOCUS       DATDSU010000026         3124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_357775, whole genome shotgun sequence.
ACCESSION   DATDSU010000026 DATDSU010000000
VERSION     DATDSU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357775"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..681
                     /locus_tag="VI981_00815"
     CDS             334..681
                     /locus_tag="VI981_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEY4478886.1"
                     /translation="MQVFTQERPWGSFRQYAEGEPVTVKTIFIRAGGSLSLQTHRKRS
                     EFWRVLRGTPQITVGQQVVAAKPGDEFEIGIGVEHRASAPDGEAEFLEVSRGEFDEND
                     IMRLEDKYGRVST"
     gene            693..2582
                     /locus_tag="VI981_00820"
     CDS             693..2582
                     /locus_tag="VI981_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478887.1"
                     /translation="MLTVHFKKKNIPHHKFPLAIGLHKRHFFMLERYVEGNMEELRIR
                     TSTNGRDFSGGKRVEIAYEDKIKEDISSCSDFRIFCADGDVVMTYIKTTRHGQHLVFA
                     VTKNLAKWVAAGTVPHVHDAGVLVPQIKKGAGHTVFWSTDRIHTISSLDLENWSPSHV
                     VRIPAWHFFEGSNFNIIGATVTPAHIIVLFESKISVDILTDNGLRDEKVGEHSYAKLG
                     VAVFSRFVPTHPVHVAELPLVEIPFKKQGMHFLGVVTINRRRERLIRLYATDEKGDVV
                     FFEFDEAAVIDEKDRKKGILKRFSKNPIIEPTEKNWEVGGTFNPTALYLDGKVHLLYR
                     AVGQDGFSYIGYATSADGKNVDERLDYPVYTPRAHFEGGNGYTKDSPGLFYSGGSWGG
                     CEDPKVTRIDDTIYMTYVAHSGYWPMRTALTSISVEDFLNRKWKWTPPMLMSPPNVGS
                     KSVVILPEKVNDQFVIFHRMWPSIMIDHVPALEFGEGKRWLKGEYRIIPRRSFWDSRK
                     LSMGSAPIKTKKGWLAIYNAVDRIDGSQYKIGAMLLRLDDPREVIARTKLPLLAPQAW
                     YENDGKPGVVYPGGAVELDQMLHVYYGGGDKVSCVATISTEELLWHLEKDSQPKIEIT
                     PVKFQ"
     gene            2808..>3124
                     /locus_tag="VI981_00825"
     CDS             2808..>3124
                     /locus_tag="VI981_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478888.1"
                     /translation="MFVLKRSLHNPILVPAKDHYWEASATFNLSLVKIGHIYHGYYRA
                     ISAPDHLRVPDQISIIGHTTSEDGFHFENRQPFISPEKEWERFGCEDPRVTYFEGKYY
                     IFYT"
BASE COUNT          867 a          680 c          749 g          828 t
ORIGIN      
        1 tgacgcacgg cagtctcgac cgacgatgag tgttgttatg ccgtgtcgtt ttgaatatgt
       61 gccatacgcg aggccgaggt gtcgcattaa ttcctcgctc aaatctgtgt tcactagacc
      121 tcgcaagtcg tatccgcgaa aaatatttgg gttgattttc atcttgattc agaaaaatgt
      181 ctttaaattg taacctataa tcgatttttt ctccgttcca catttccaaa gacgccgaat
      241 tttagctcat ttttcaaggt ctctttgggc attatgagac ttatgcacag aaaaagccca
      301 aaaatgctgg tttttctgct aaaattatag gcaatgcaag tcttcactca agaaagaccg
      361 tgggggtcat ttagacagta tgctgaaggg gaacctgtca cggttaaaac gatttttatc
      421 cgagccggtg gatcgctttc tttgcagacc catagaaaac ggtctgaatt ttggcgagtg
      481 cttcggggaa caccccagat taccgttggg cagcaggttg tggcggcaaa accgggtgac
      541 gaatttgaaa tcggcatagg cgtcgaacac cgtgcaagcg caccggacgg cgaagccgaa
      601 tttctcgaag tgtcacgtgg tgaattcgac gaaaacgaca ttatgcgtct tgaagacaag
      661 tacggcaggg tgtcgacata gagttatatt caatgctcac cgttcatttc aagaaaaaaa
      721 atattcctca tcacaagttt ccgctcgcca ttggactcca taagcgtcat tttttcatgc
      781 tcgaacgcta tgtcgaagga aatatggagg aactccgtat cagaacaagc actaacggtc
      841 gtgattttag cggaggcaaa agagtagaaa ttgcctatga ggataaaata aaagaagata
      901 tttcgagctg cagcgatttt agaatttttt gcgcagacgg tgatgtcgtg atgacctata
      961 taaaaactac acgacatggt cagcatctcg tttttgcagt cacaaaaaat ttggctaaat
     1021 gggttgcggc ggggactgtg ccgcacgtgc atgatgcggg agttcttgtg ccgcaaataa
     1081 aaaaaggagc gggtcacacg gttttttgga gtactgatcg aattcatacg atttcgtcgc
     1141 ttgatcttga aaattggtca ccttcacacg tggtgcgaat tccggcgtgg catttctttg
     1201 aaggttcaaa tttcaatatt atcggtgcga ctgtcactcc agcacatatt attgttttat
     1261 ttgagtcaaa aatttcagtt gatattttga cggacaatgg attgcgcgat gaaaaagtcg
     1321 gtgaacattc atacgcgaaa ctcggtgttg ctgttttttc tagattcgtg ccgacgcacc
     1381 ctgttcacgt tgctgaactg ccgcttgtag aaattccttt caaaaaacaa ggaatgcatt
     1441 ttctgggtgt tgttacaatt aatcgccgac gcgagcgctt gatacgactt tatgccacgg
     1501 acgaaaaagg tgacgttgta tttttcgaat ttgatgaagc tgccgtcata gacgaaaaag
     1561 accggaaaaa aggtatatta aagcgctttt cgaagaatcc gattattgag ccgacggaga
     1621 aaaattggga agttggcggc acgttcaacc cgacagcgct ctatcttgac ggaaaagttc
     1681 atctgctcta ccgagccgtt ggccaggacg gtttttcata tattggatac gccacgagtg
     1741 ctgatggaaa aaacgtcgac gagcgtcttg actatcccgt ctatacaccc cgtgctcatt
     1801 ttgaaggcgg aaacggatac acaaaagata gccccggact cttttattca ggaggcagtt
     1861 ggggaggctg tgaagatcca aaagttacgc gcattgatga cacgatctat atgacgtatg
     1921 ttgcccattc aggatactgg ccgatgcgca cggcgcttac ctcaatttcc gtagaggact
     1981 ttctcaaccg caaatggaag tggactccgc ccatgctcat gtctccgcca aatgttggaa
     2041 gcaaaagcgt ggtgattctt ccagaaaaag ttaatgatca attcgtcatt ttccatcgta
     2101 tgtggccgag tataatgatt gatcacgttc ccgcactcga gtttggtgaa ggaaagcgat
     2161 ggctcaaggg agaatacagg attataccga ggcgttcctt ttgggatagc cgtaaacttt
     2221 ctatgggatc agctccgatc aaaacaaaaa agggctggct cgctatttat aatgcagtcg
     2281 acagaataga cggatcacaa tataaaatcg gggccatgct cctgcggctc gacgatccga
     2341 gagaggtcat cgccagaacc aagttgccgc ttctcgcacc tcaagcctgg tacgaaaatg
     2401 acggaaagcc cggcgtcgta tatccgggtg gagcggtaga gctcgaccaa atgcttcacg
     2461 tctattatgg cggaggggat aaagtctcat gcgtcgccac cattagtacc gaggagttgc
     2521 tctggcatct tgagaaagat agccaaccga agattgagat cacgccagtc aagtttcaat
     2581 gaaaattaat gagcaaagcg aatataattt tcttgacccc gtcgtaattt tgcgagagta
     2641 ataccacgcg aagcgcgagt gtcgaataga cacataccta tacgggcgca gcgcttcgcc
     2701 cgataaggag agcacctatt cggtgcgcgc actgcgcaga attatgaaat ggcaaaatta
     2761 cgacggggtg ctcaaatttt tattcattaa cattcataaa atttggcatg tttgtcctca
     2821 agcgctctct acacaacccg attctcgtcc ccgcaaaaga ccattactgg gaagcctctg
     2881 ccactttcaa tttgtctctc gtaaaaattg gtcacatata tcatggctat taccgtgcca
     2941 tctcggctcc agaccatctg cgagtgcctg atcaaatttc aattatcggc catactacaa
     3001 gcgaagacgg gtttcatttc gaaaatcggc aacctttcat cagtcctgaa aaagagtggg
     3061 agcgtttcgg gtgcgaagat ccgcgagtaa catattttga gggcaaatat tatatttttt
     3121 acac
//
LOCUS       DATDSU010000027         3449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1362250, whole genome shotgun sequence.
ACCESSION   DATDSU010000027 DATDSU010000000
VERSION     DATDSU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362250"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..611)
                     /locus_tag="VI981_00830"
     CDS             complement(126..611)
                     /locus_tag="VI981_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478889.1"
                     /translation="MGKKINTKEIGKISRMVAYPKPNLPTPAVLVACCLIDFLARNLK
                     PKSRCDRFVRFIERRMKKTHYQLTQNDSLKKSQLKRVKCDFKGHEGKKCKTSAEILYR
                     HVRSGLVHNYFGSEGYIIINRPNKKQMGVIVDQSKKYKKYALVLNGSAFIKDFLSSLP
                     K"
     gene            complement(649..2280)
                     /locus_tag="VI981_00835"
     CDS             complement(649..2280)
                     /locus_tag="VI981_00835"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEY4478890.1"
                     /translation="MKKLTTLSILLISLAFVVPAQASAATSAGVTPGSFFYFFDTTFE
                     NISLFFTFDSENKARKALEYADERLAEIEAVAEEKNPNGVKTAIANYESNIALATEKS
                     KEVKDKGQAESLLTSISDNTAKNQEVLAAVLIKVPEEAREAITRAIEVSRKGQEEATR
                     QITELKKELSELKQELENLKGELKDKEEEPKAGGDKKDEQTKAVVKLKSEIESLKKKV
                     SEPSQKETKTESKKEEPKNSIVNLPSGAVVEMDVNGNIIRTIKEAPVSTSQNSYTPPQ
                     SSAQQSQTQTQSQPQTNQTQTSSQQQTEAPKITSVNISPNQTSATFEWQTDKPTQSKI
                     FISGVGLSAKIFNSASGLSTRHIATVTGLLENATYTYEIEAIAEGTSVVKKQGSFETL
                     PKPVLKVKIDPVSYSGPVPSASLSNKQIPMFTSNTPTPKLRVKMDIYTTEHGLSFDYL
                     QLNGVKFQKISDNVTQNNGKYATSTYEYTLSVSPTQSLTLTNVLYNETYPYPNFELTL
                     TLEKDGEWVCLDNYSSCEFPTFPITNVVTVNPRSQ"
     gene            complement(2285..2506)
                     /locus_tag="VI981_00840"
     CDS             complement(2285..2506)
                     /locus_tag="VI981_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4478891.1"
                     /translation="MVDTIRTKEYGDFVGKLRKARLEAGLRQIDVAKKLRRTQSYVSR
                     VEVGEQRLDILELQKFAKLYGKDINYFIK"
     gene            2959..>3449
                     /locus_tag="VI981_00845"
     CDS             2959..>3449
                     /locus_tag="VI981_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY4478892.1"
                     /translation="MSKPKYFLYARKSTEDDDKQIMSIEAQLFELREFARKENLEILE
                     EFQESKSAKSPGREVFGDMMMRIEKMAGVGILAWHPDRLARNSIDGGRIIYAVDTSKI
                     VSLRFPTFWFEPTPQGLFMLQVAFGQSKYYSDNLKQNVERGMRQKLRRGEWLTRAPFG
                     YVN"
BASE COUNT          819 a          802 c          805 g         1023 t
ORIGIN      
        1 tttgatatgt gcttgcccca aaccctcccg tgcgcgcaca acaacccccg ctgtttttag
       61 gagaaattat aaaacggcgg ggggtgctcc ctaatgctct attggtccgc gaatgcgggc
      121 aaattttact ttggcaacga cgataaaaag tcttttatga acgccgagcc gttcagaacg
      181 agagcatact ttttatattt cttgctttga tcaacgataa cgcccatttg ctttttgttt
      241 ggcctattga taatgatata tccctccgag ccaaaataat tatggactaa accggaccta
      301 acatgtcggt ataaaatttc agcagaggtt ttacactttt taccctcgtg gcccttgaaa
      361 tcgcatttta ctcttttgag ctgcgatttt ttgagagaat cgttctgcgt aagttggtaa
      421 tgggtctttt tcattcttct ttcaataaat ctgacaaaac ggtcgcagcg actttttggc
      481 tttagatttc gagccaagaa atctatcaga caacaagcaa caagaaccgc tggagtgggc
      541 agatttggct tcggatatgc caccattctc gaaattttcc cgatttcttt tgtattgatt
      601 ttctttccca tgtcaatcta tgcaggtaat gtgcacccat ggccatgatt actggctcct
      661 cggattgacg gtaacgacat tagtgatcgg gaaagtagga aactcgcagg aactatagtt
      721 atcgagacaa acccattcgc cgtccttttc aagggtgaga gtcagctcga agttaggata
      781 tgggtaggtt tcgttataca acacattagt cagcgtcaga gactgtgttg gactaacgct
      841 caatgtatat tcataagtgc ttgtggcgta cttaccatta ttctgggtta cgttatccga
      901 gattttctga aacttaaccc cgttcaattg caggtaatca aacgacaacc catgctccgt
      961 agtataaata tccatcttca cacgcaactt tggcgttggc gtattgctag taaacatagg
     1021 aatttgctta ttgctcaagg aagcggaggg cacaggcccg ctataactta cagggtcgat
     1081 ttttactttt agtaccggct ttggtaaggt ttcaaaactc ccttgcttct tcaccacact
     1141 tgtaccctcg gcaatcgcct ctatttcgta tgtgtatgtc gcgttctcca ataaaccagt
     1201 gactgtcgcg atgtgacggg tagagagtcc gcttgcggag ttgaaaattt ttgccgacaa
     1261 ccctacgccg gaaatgaaaa tttttgattg tgtaggttta tcggtctgcc actcaaatgt
     1321 agccgatgtt tgattcggtg aaatattcac tgatgtaatt tttggtgctt ctgtctgttg
     1381 ttgtgaagaa gtttgagtct gattagtctg tggttgagat tgtgtctggg tctgagattg
     1441 ttgagcacta gattgcggcg gtgtgtaaga gttttgtgat gtagaaacag gagcttcctt
     1501 gatagtacga ataatatttc cgttcacgtc catttccacg accgcgccgg acggcaaatt
     1561 cacgatgcta ttttttggct cctctttctt gctctcggtt ttagtttctt tctggctcgg
     1621 ctcgcttact tttttcttca gactttcgat ttcgctcttt agtttaacta ccgcttttgt
     1681 ttgctcgtct ttcttgtcgc cgcccgcctt gggttcctcc tctttatctt tcaattcacc
     1741 ttttagattt tcaagttctt gcttaagttc cgaaagctct tttttgagtt ctgtaatttg
     1801 cctcgttgct tcctcctgtc cctttctgct tacctcgatt gctcgcgtga ttgcttctct
     1861 cgcttcttcg gggactttga tcaaaacggc ggccaaaact tcttgatttt ttgcggtatt
     1921 gtccgaaatc gaagtgagca aactttccgc ttgtcctttg tcttttacct cttttgattt
     1981 ttccgtcgcc aacgcaatat tactttcgta attggcgata gctgttttaa ccccgttcgg
     2041 atttttttct tccgcaacgg cttctatttc ggccagcctc tcgtcggcgt actccaacgc
     2101 cttccttgct ttattttcag aatcaaaagt gaaaaagagg cttatgtttt caaaagtggt
     2161 gtcaaagaag tagaaaaaac tccccggcgt aacgcctgcg cttgtagccg cggacgcttg
     2221 cgctggtacg acaaaagcga gtgaaatgag aagtatcgac agcgtggtta gttttttcat
     2281 ggctctattt tataaaataa ttgatatcct taccatacaa cttggcgaac ttttgcaatt
     2341 ccaagatatc caacctttgt tcgccgacct cgacgcgaga cacatacgat tgcgttcgcc
     2401 tcaatttctt agcgacatca atttgccgca agcctgcctc aagccgagcc tttcgcagtt
     2461 tgccgacgaa gtcaccatat tccttggttc gtattgtgtc taccatagat agtatcaacg
     2521 tatatgctgg attgcaatac ctaaactttg catattgagg tagactaacg gaagccgccc
     2581 ctcgcctttg ccgcccattc aattttaaaa aatccccccc ccgcaaaaaa tcagcatgga
     2641 gaggagcggc aaggcgagag ggcgggacgc gcggtactcc gcgcgtggcg ggcttcccac
     2701 gcgatcggag cgtacttttt cgggtcaaag ccaccacgca cgcggagcgt gcgaagtggg
     2761 ttcgcgcatc gtgtagaatt agatcacatt cagaaacttc cgccggagtg agggcgaatg
     2821 agccgcgcgc ctgcggcgcg cggcggggcg ggcgggcgaa atgcatgagg cgggtgcggg
     2881 cgaattcgga gggcgggcag ggatgcaaga aatgagaccg gaaatagtct cgtcagagta
     2941 aatccaaaat ctatttatat gtccaaacca aaatattttc tatacgcgcg aaagtccacc
     3001 gaggacgacg acaagcagat tatgagcatc gaagcgcagc tgttcgagct tcgtgagttc
     3061 gcgcgtaaag aaaatcttga aattctggag gaatttcaag agtcgaaaag cgcaaagagt
     3121 ccgggacgtg aagtcttcgg cgatatgatg atgcgcattg aaaaaatggc gggtgtcggc
     3181 atcctcgcgt ggcatcccga caggttggcg cgcaatagca tagacggcgg gcgcatcata
     3241 tacgccgtag acacgtctaa aatcgtctct ttgcgctttc cgacattttg gtttgaacca
     3301 acaccgcagg ggctatttat gctccaagtg gcattcggcc agtcgaaata ttattccgac
     3361 aatctcaagc aaaatgtcga acgtggtatg cgccaaaaac tgcgccgagg cgagtggcta
     3421 acccgcgcgc cattcgggta tgtgaataa
//
LOCUS       DATDSU010000028         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2366626, whole genome shotgun sequence.
ACCESSION   DATDSU010000028 DATDSU010000000
VERSION     DATDSU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366626"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VI981_00850"
     CDS             <1..201
                     /locus_tag="VI981_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478893.1"
                     /translation="KNKDLIAIPKTTFEEFLIWQRKTKSSKKFKPTLSEKKILIKARK
                     NFVKRKFVSITELRHELGRRRR"
     gene            176..433
                     /locus_tag="VI981_00855"
     CDS             176..433
                     /locus_tag="VI981_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY4478894.1"
                     /translation="MNWDVVVDKQIEKFLKKIGRRDKERLIGAMQEFSADPYAGDVEK
                     MEGEENVWRRRIGAFRIFYELYPSKKIVYVFSIKRRASNTY"
     gene            complement(654..1352)
                     /locus_tag="VI981_00860"
     CDS             complement(654..1352)
                     /locus_tag="VI981_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478895.1"
                     /translation="MDTTALIQKLHSGGFALTRPATTVSFLRHANAIAADGGHDSNRL
                     ISDKGRKQCAVMRETHTRFMKFNAIYRSPLPRVDQTLMGLGIDTGDAVIIPQLVIESG
                     LETHQCGVIDAAYEILGGVGLASIMKYKEVAYAMDVYAATALEALYEQFEALPQWTVA
                     VPVAKEWGPARFHVIAAGHGWFNPLLAYYLAGSNEVAKRHLLQHAMGECEIIQVGVND
                     TGEPHWERFACPSV"
     gene            1707..>2950
                     /locus_tag="VI981_00865"
     CDS             1707..>2950
                     /locus_tag="VI981_00865"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEY4478896.1"
                     /translation="MAKPRPNFYVLFAASLVVAVLVSVSVVGVNELGNFFTEKNGEPI
                     RRSQLAAVQSVDLISNIGTASNWTTFGGTWTLGSDGIYRLTRPVLREGDGNGNIAAND
                     TLIPNEFTLTTNAMVVATSHEWNDFSVVFGFKDINNYYYVSFNEYDDENTNGIFKVSG
                     GVNSELFNIGKAGSENAYFDFDDNAEEKLYEGPGSISAGVTYRVKIERKGTGLEVFLN
                     EVSVANIQIPESLAGKVGYGTRNDSANFSNLRVTEMVNVPDPTYALTVTKSGSGTVSA
                     QGISCGNDCSETYALNTSVTLTATPTADSQFSGWLGSCSGVGSCMVTMNSAKSISATF
                     TTPSASNTYILTIVRNGSGTVSGNGISCGTDCSESYEENSSVTLTATPAAGYTFAGWS
                     GACSGSALCTVSINGPNTVIAV"
BASE COUNT          822 a          602 c          735 g          791 t
ORIGIN      
        1 aagaataagg atcttattgc tataccgaag acgacattcg aggaattttt gatttggcaa
       61 agaaagacaa agtcctctaa aaaatttaaa ccaacgttat ccgaaaagaa aatacttatt
      121 aaggcgagaa agaattttgt aaaacggaaa tttgtatcta taacagaact ccgacatgaa
      181 ctgggacgtc gtcgtcgata aacagataga aaaatttcta aaaaaaatcg gaaggcgcga
      241 caaggaacgt ctaattggag cgatgcagga atttagcgct gatccgtatg cgggagatgt
      301 cgaaaaaatg gaaggcgagg aaaacgtttg gagaagacga atcggcgctt tcaggatctt
      361 ttacgagctt tacccaagca agaaaattgt atacgtattt agtattaagc gtcgtgcatc
      421 aaatacatat tgataaccat tcatcaatgt attgcatcat cggcacgaag attgccacga
      481 aggttttgaa ggatggtgat atggtggagg tggacgccag tgaaggaatt gtcagaattc
      541 ttaaataagt cttattctag tattttgcaa aattacaaaa taagaaagaa acgataagaa
      601 aaaaaagtct acaacctcgt tggttgcaga ctgtatgact gtgatgtgtg tgattaaaca
      661 ctcgggcaag cgaaacgttc ccaatgcggc tcgccggtgt catttacgcc gacttggatg
      721 atctcgcact cgcccatggc gtgctgtaag aggtgacgct tggccacttc attcgagcca
      781 gccaagtaat aggcgagcag agggttgaac cagccgtgac cagctgcgat aacgtgaaag
      841 cgcgcaggac cccactcttt cgcgacgggt actgccactg tccattgagg gagtgcctcg
      901 aattgctcgt agagagcctc gagcgctgtt gccgcgtata cgtccatcgc gtatgcaact
      961 tccttatact tcatgatgct ggccaggccg acgcctccca ggatctcata ggccgcgtcg
     1021 ataacgccac actgatgagt ttccaggccg gattcgataa cgagctgagg gataattacg
     1081 gcgtccccag tgtcgatgcc gagacccatg agggtttgat cgacccgggg aagcggtgat
     1141 cggtagatgg cattgaactt catgaatcga gtatgcgttt ctctcatgac agcacactgt
     1201 tttctccctt tgtcggatat gaggcgattc gagtcatgac caccatctgc cgcgatggca
     1261 ttggcatgac gcaaaaatga gacagttgtg gcgggccggg tgagggcaaa gccgcccgag
     1321 tggagtttct gaatgagtgc ggttgtgtcc atagcgagtc tgggtgctgt attttcaggt
     1381 agtctgaaca gacgcggggt tttcaaatgc gaaaactcaa ggttcagact gattaaacgc
     1441 tagcatattt tatgaggttt gtcaaagcct cataaggccc aaagatggct ctagatgtgg
     1501 atattcctgt attttttatc aaggttttgc tatatgagca ctcgcatatc tttgttggtt
     1561 cctatttaag taaaattttc aattattaac tagtcactct catatgcgag cattccgacg
     1621 gtcgtctcat atattgccgt cactcgcact gaatgaaggt ttccccttca ttagctgctc
     1681 gttaggagat ataatttcag cattgtatgg cgaaaccgcg tccgaacttt tacgtattgt
     1741 ttgcggcctc tcttgtggtg gccgtccttg tatccgtctc tgtcgttgga gtgaatgaac
     1801 ttggaaattt ctttaccgaa aaaaatggtg agccgattcg tcgatctcaa ctcgctgcgg
     1861 tccaatctgt agacttgata tcgaatattg gaactgcatc aaattggact acatttggtg
     1921 gcacatggac attgggaagc gatggtattt acagactgac aaggcctgta ttgcgcgaag
     1981 gtgatggaaa cggaaacata gccgccaatg acacacttat tccaaacgaa tttacactaa
     2041 caacgaatgc tatggtggtg gctacgtcac acgagtggaa tgatttttct gttgtttttg
     2101 ggtttaagga cataaataat tactattacg tcagtttcaa cgaatatgat gacgaaaaca
     2161 caaatggaat ttttaaggtg tctgggggag ttaactcgga attgtttaat atcggcaagg
     2221 ccggaagtga aaacgcgtat ttcgattttg acgacaacgc agaggaaaag ttatatgaag
     2281 gtcctgggtc aatatcggct ggcgttacgt atcgtgtaaa gatagaaaga aagggcaccg
     2341 ggcttgaagt ttttttaaat gaagtttccg ttgcaaatat tcagattccg gaaagcctag
     2401 ctggtaaagt tggttatggc acgagaaatg actctgcaaa tttcagtaac cttcgggtaa
     2461 ccgaaatggt caatgttcct gatccgacgt acgcgctaac tgtaactaaa tctggatcag
     2521 gcactgtgtc tgctcaaggt atttcatgtg gtaatgattg ttcggaaaca tatgctttga
     2581 atacttcggt tacgttgaca gcaactccca ctgccgactc tcaatttagt ggctggttag
     2641 gctcatgtag tggcgtagga tcgtgtatgg taaccatgaa tagcgcaaag tcaataagcg
     2701 ccaccttcac cactccttca gcttcgaata cttatattct gacaatagtt cgaaacggat
     2761 caggtacagt ctcgggaaac gggatttcat gcggaacaga ctgcagtgaa tcctatgagg
     2821 aaaacagctc tgtcacactt actgctactc cggccgcagg ttacacattt gccggctgga
     2881 gcggtgcctg tagcggcagc gctctctgca cagttagcat caacggaccg aatactgtca
     2941 ttgcggtttt
//
LOCUS       DATDSU010000029         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2032765, whole genome shotgun sequence.
ACCESSION   DATDSU010000029 DATDSU010000000
VERSION     DATDSU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032765"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VI981_00870"
     CDS             complement(<1..134)
                     /locus_tag="VI981_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEY4478897.1"
                     /translation="MLDLKTMKSALEQLEAERAIPKEKIIEAIEAALAAAYKKDYGKK
                     G"
     gene            362..625
                     /locus_tag="VI981_00875"
     CDS             362..625
                     /locus_tag="VI981_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478898.1"
                     /translation="MNWIWRILRALFRTPRRAATTLGVLLVLGLLQEFRPDAAQSLST
                     GASHTIAGGMGVVLSAIMPIVQMFLLPIVLLLIIVWMVKKFLR"
     gene            646..939
                     /locus_tag="VI981_00880"
     CDS             646..939
                     /locus_tag="VI981_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478899.1"
                     /translation="MKFALEFASFFAVVVLVFGSIVALLVASVWAAGEVGLKTSLWLF
                     LGSTVVGGLVGGLWHGTISDGVGGALASGILSLVVLILAALVQKYRERNPASR"
     gene            complement(1027..1830)
                     /locus_tag="VI981_00885"
     CDS             complement(1027..1830)
                     /locus_tag="VI981_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4478900.1"
                     /translation="MYDQQLVFMGLTKRQAKIYDVLIREGRKTAGDIAKAATISRPLV
                     YQALDELAALKLVGKEDKPGKVATFSPQHPFVLKDIVAQKRDEAEKAHLAVDSILGKM
                     ISDFHLISGQPGIHIYEGFDGIRELYENILFERKPISLFRSYKDKSDPKTTAILREFI
                     ALEVKYGIKTRAITPTLKNKAGYSLDEDRNNLTERREIPFSKFKLPAQIVLFGNKVAL
                     TSYQDEIITTIIENRAIGASFSVIFELLWEIASLPPYPSSTAILSVKQD"
     gene            2507..2827
                     /locus_tag="VI981_00890"
     CDS             2507..2827
                     /locus_tag="VI981_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478901.1"
                     /translation="MYTWKKAGYNYPRFDYPDRIYDGFEISAIVADGKCDGRWQAEIF
                     WGGYRSLQLGLGHGTTPQVALEAACRQAETYDWQGAFGVNGVEHVQQLREWVTSLCRT
                     HAIP"
     gene            2969..3337
                     /locus_tag="VI981_00895"
     CDS             2969..3337
                     /locus_tag="VI981_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478902.1"
                     /translation="MNILEKISSSGKIRNESTDNLKAEYETLKGEADTLQEETLKQYS
                     EEMSAWRKYEALTKSAGWTPWDPSQGDPAVIEEVERLFALLMDFNTKSQHRDGRLAGI
                     YGRLHEIGFELHVRGRVDAQ"
     gene            3429..>4115
                     /locus_tag="VI981_00900"
     CDS             3429..>4115
                     /locus_tag="VI981_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478903.1"
                     /translation="MSENESPKKDEEAGGRMTHPASSEKFMGEIARSVETLEKEFDVD
                     LSSLPESLVNEIVLSFQKEEPEHLERIKQFVDDGGYAQSKFYRLLTFFTLPEEFREDL
                     LSAMSRIAPQNLGFVFKKYVELQLSFSEIKNYLKGVSSSEAATVEELTEKVRSNYLQR
                     VHEQIFRNQGGLIAEYGRISPEDVSSLSHVAGSLHRELERIDSQRLILLASLKILHDE
                     DAHVNLKEILD"
BASE COUNT         1007 a          938 c         1077 g         1093 t
ORIGIN      
        1 cctttcttgc cgtaatcttt tttgtacgcg gcggcaagcg cggcttcgat cgcctcgata
       61 attttttctt tcggtatcgc acgctctgct tccaactgct cgagcgcgct tttcatggtc
      121 tttaaatcaa gcatgatagt cagtagtcag tagtcagtag tcagtagtca gtagaaatgc
      181 aattgcaaat cccagattgc tgcttccaac tccaaattaa aaaataaacc aggataccca
      241 tacggtagcc tgttctgatg aagtaaagga tagcaataaa gacagatatc gtcaagaagt
      301 tgactaaacc gacgaaccat gttacactca aaacaacttt ctagcacatt gagagatcaa
      361 aatgaattgg atttggagga ttctgcgggc gctgttccgg acaccgcgca gggcggcaac
      421 tacgctcggc gtgctacttg tcctgggact tttgcaagag ttccggccgg atgcggcgca
      481 gtcgctttcg acgggtgctt cccacacgat cgctgggggc atgggcgtcg tattgagcgc
      541 catcatgccg atcgtgcaga tgtttcttct gccgatcgtg ttgctactca tcatcgtgtg
      601 gatggtgaaa aaatttctgc gctaacccgc gcgaaaggag aagaaatgaa atttgcattg
      661 gagtttgcgt cattttttgc agtagttgtt ttggtgtttg ggagcatcgt tgcgctgctt
      721 gtcgcgtcgg tgtgggctgc cggcgaagtg gggttgaaaa cgagcctgtg gcttttcctc
      781 ggttcaacag tcgtcggcgg attggttgga ggtttgtggc acgggacgat ctctgatggt
      841 gtcggtggtg ctcttgcgtc aggaattctc tcgttggtag tactgatcct cgcagcgctc
      901 gtgcaaaaat accgtgagcg gaatccagcg tcccgctaac atctgttcac gcgttcttct
      961 cggcaggccc cacggcctgc tttttgtacc ccgacatgct cacgtttctc gaacgatttt
     1021 gtgtttttaa tcttgtttga cggataaaat tgccgtactt gacgggtatg gtggtaggct
     1081 ggcaatttcc cacaggagtt cgaatatgac agagaacgaa gcgccgatcg ctctattttc
     1141 tatgatagtg gtaatgattt cgtcttggta cgacgtgagc gccaccttgt tgccgaacaa
     1201 aacgatttga gcgggaagct tgaacttact aaacggaatt tcccgtcttt ccgtgaggtt
     1261 gttgcgatct tcgtcgagac tgtagcccgc cttgtttttt agcgtcggcg tgatggctct
     1321 tgtttttatt ccatatttca cctcgagtgc aataaactcc cgcaggatcg ctgttgtttt
     1381 cgggtccgac ttgtctttgt atgaacgaaa aaggctgatc ggtttccgct caaataaaat
     1441 gttttcgtac agttcccgaa tgccgtcgaa tccttcatag atgtgaatgc caggttgtcc
     1501 cgagatgagg tgaaagtcag agatcatttt gccgaggatc gagtctacgg ccaagtgcgc
     1561 cttttctgcc tcatcccttt tttgggctac aatgtccttt aagacgaaag gatgttgcgg
     1621 actgaacgtt gctactttac ccggcttatc ttctttccct accagtttta aggccgcaag
     1681 ttcatcaagg gcttggtaca cgagcggccg ggaaatagtc gccgccttcg caatatcgcc
     1741 cgccgttttg cgcccctctc ggatgagcac gtcgtaaatt ttcgcctgcc gcttggttaa
     1801 acccatgaat acaagttgtt gatcgtacat atttgtaaaa aatttattac aaaactatta
     1861 tataaaaata tgcatttaaa agcaatacta gattttgtaa ttacaaattt acattttctt
     1921 tttgacaaat atcgcaaaag cggtatgatg ggcacataag ttcattgagt aaatggctcg
     1981 gtgcctgggc taccgatggg tttgtccggg acttgcactt gaggagcagg tgagctcgtc
     2041 actcacagtg cggaaggacg ctctcgttac aaacgagggc gggtcggcaa gcagccagca
     2101 cctactacag cgatacgtcg ctgtggcgtt cccttcgagg gaggaatgta agaggttgac
     2161 aaaccgcatt ccctcgaatt tttgtttccg ggttggatag gagcccggat ggtcgcgcgg
     2221 tcgaggttac tcatgtggcc actgagcggg gttgtctggc gttgctagtg acgcaccaac
     2281 gacacctgga atctcaccga gtgaacaagc tgaatcaagc cagcagtggc gctcatggtt
     2341 cagaaggaca ccacgaagcg gtgtctaaaa atatctgttg gcgagcgttt gcgctcgcca
     2401 tcttcataaa gtttctgaaa tgggtcagga acttttagag atttgttctt tgagtcatgg
     2461 cactctgcca atctgcgcct cgggagtctg gggttaggag tagaacatgt atacatggaa
     2521 aaaagccggc tacaactacc cgagattcga ttatccggac cgcatatacg acgggttcga
     2581 gatctcggcc atcgttgctg acggtaaatg cgacggaaga tggcaagccg aaatcttttg
     2641 gggaggttac cgttcgttgc agcttggcct cggacacggg acaactcccc aggtcgccct
     2701 tgaagcggcg tgccgtcagg cggaaacgta tgactggcag ggggcgtttg gcgttaacgg
     2761 cgtcgagcac gtacagcagc tacgggagtg ggtgacgagt ctttgtcgca cccacgcaat
     2821 cccttagctt catgggcgaa cgcgctgttc gccatcttta taaagatgct gattgccgct
     2881 ttggatcaga gccttttacc gattatttcc ttaactaaag accgagagag acctgcgtga
     2941 cgacctctct ttgtgttaaa ctataggcat gaacatcctc gaaaagatat ccagttctgg
     3001 aaagattcgg aatgagagca cagataattt gaaggcagag tacgagacct taaaaggaga
     3061 ggcggacacg ctacaagagg aaacactcaa acaatattct gaggaaatga gcgcatggag
     3121 aaagtatgaa gcacttacga agtcggctgg ctggactccc tgggacccat ctcagggcga
     3181 cccggccgtt atcgaagagg tagagagact cttcgcattg ctcatggact ttaacactaa
     3241 aagccagcat cgggacgggc gcctggccgg catatatggt cgcttacatg aaatcggttt
     3301 cgagctccac gtgagaggga gggtggacgc gcaataggaa agcgaaaaaa tcttcagtca
     3361 cgttttccac aagcaggccc cacggcctgc ttttttgcag agagtgtctt tggtgtatgc
     3421 ttaaacgcat gagcgaaaat gagtcaccca aaaaagacga ggaagccggc ggcagaatga
     3481 cccaccccgc atcttctgag aaatttatgg gggaaattgc ccggtcggtg gaaacactcg
     3541 aaaaagaatt tgatgtggac ctttcttcgc ttccggagtc tcttgtgaat gaaattgttt
     3601 tatcgtttca aaaagaagag ccggaacacc tcgaaagaat aaaacaattt gtcgatgatg
     3661 gcggctatgc gcagagtaag ttttatcgac tcctcacatt ttttactctc cccgaagagt
     3721 ttagagaaga tttactctct gccatgagcc gcattgctcc acagaacctc gggtttgtct
     3781 ttaaaaaata tgtcgagctg caactctctt tttccgaaat aaaaaattat ttaaaagggg
     3841 tctcaagtag cgaagcggca acggttgagg aattgactga aaaagtacgc agcaactatc
     3901 ttcagcgggt gcatgaacag atttttcgta atcagggcgg gcttattgcg gaatatggac
     3961 gtatttcccc tgaagacgtg agtagtttgt cgcatgttgc aggcagtctt catcgagagc
     4021 ttgaaagaat tgattcgcag cgtctcatcc tgcttgcctc tctcaaaatc cttcatgacg
     4081 aggatgccca cgtaaatctt aaggagattc tcgat
//
LOCUS       DATDSU010000030         3946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2198408, whole genome shotgun sequence.
ACCESSION   DATDSU010000030 DATDSU010000000
VERSION     DATDSU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2198408"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..560)
                     /locus_tag="VI981_00905"
     CDS             complement(210..560)
                     /locus_tag="VI981_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478904.1"
                     /translation="MENHMQTFGKKLLTRLAPILGAGAIGVCPLCWIGSASLLTYLGL
                     GALIPAWRWLAFGLIALGAVGFIFDYRAHKNPKPLILLTLGAILFMWDGMCLRAHGAR
                     GGFGDLAHSLLLRP"
     gene            complement(569..1483)
                     /locus_tag="VI981_00910"
     CDS             complement(569..1483)
                     /locus_tag="VI981_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478905.1"
                     /translation="MAKTSKKYVWILVAFVVAGGIVILMRQNNSGSVSTQSNLIPEQS
                     ISHGHGLAVDVADPSKLWIATHYGLLLLESEKDLYRVGKSRDDYMGFSAHPTGPSIFF
                     TSGHPARGGNLGFQRSDDGGATWKRISNGANGPVDFHALAVSPVNPNLIYGWYAGNLQ
                     RSEDQGASWKIINREIVAAYLSADSENENKVYAATPRGQGVIVSEDKGTTWQALSPAL
                     EGGAVSVVAVHPQNSKTLLAFSERLGGLRKSGDSGQTWTQVNESFGGEAVLHIAFSKI
                     DANIVYALTHENKLFKSSDAGDMWVQIR"
     gene            complement(1497..1997)
                     /locus_tag="VI981_00915"
     CDS             complement(1497..1997)
                     /locus_tag="VI981_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478906.1"
                     /translation="MSHALNIFLFGIREPFKKTNYIFLFLVSFFVFFALFVFIPVWTV
                     AGNTLATQLEIFTARDYVMLILLSSLSSLFITMQVYVMRQKRKIGGVSTATAGGLGTL
                     FAGIAGTAFCASCLAPLFAIFGIGFGGVIFVLEYRFYFVAGITLLMLIAIYLTARKIN
                     KVCTSC"
     gene            complement(2003..3430)
                     /gene="merA"
                     /locus_tag="VI981_00920"
     CDS             complement(2003..3430)
                     /gene="merA"
                     /locus_tag="VI981_00920"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEY4478907.1"
                     /translation="MKNFDLIIIGGGAGAFAAAIRANELKAKTALVNAGLPLGGTCVN
                     VGCVPSKTLLYAGEILHHAKHHGTPGIELEVKNFDFQKVVQDELSLVEKLRQEKYEKV
                     LKNLEYVTAIEGKAKFVGVDEIEIVSERNPVAERSSLRGKKFIIAAGSTANVPEIAGI
                     RGVGFLSHIEALRLPRQPKELVIIGAGPLGLEFAQMFARFGTKVTILQRGDSIFPHSE
                     KALTDRLAEILAKEGITIKTNVEVKSVRKENGKKVILYTINGAQEETVADEILLAAGK
                     TPNTQGLGLDTAGIEIDKRQAVVVNQNFQTSNKNIFAVGDVTGAPVRLETTAGREGTL
                     AAENILKGTQLFIDYNTVPYAIFTDPQLAGVGFTEDEQMKQMGVCTCRTVSFADVPKA
                     IIIHRTQGMIKMAIHPQTKQILGVHILGAHASELIAEAMMLVKNKNTIDDVVNSLPMF
                     PTLSEAIKIAALSFTKDISKLSCCI"
     gene            complement(3432..3746)
                     /locus_tag="VI981_00925"
     CDS             complement(3432..3746)
                     /locus_tag="VI981_00925"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4478908.1"
                     /translation="MQTKDIQKIRKELNDRKAILRCAEEFNMVGDPTRLKICYLLCRH
                     KELAVGEISEIIGVSISAVSHTLKKLQKAGLVENRREFRTVYYKLKQTPLASMLRERL
                     TT"
     gene            complement(3718..>3946)
                     /locus_tag="VI981_00930"
     CDS             complement(3718..>3946)
                     /locus_tag="VI981_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478909.1"
                     /translation="PSKYSGSVAGRNADSVLSKLFSGGDLAKPRLTFSAFIRAGVLIF
                     GGLHVHKTLVVFFVLICYHLNVYANERYSKN"
BASE COUNT         1043 a          971 c          877 g         1055 t
ORIGIN      
        1 gccctccaag tattcgggca gtgtggcggg gcgaaatgcg gactcggttt tgtcaaaact
       61 attttctggg ggggatttag caaaacctcg gctgactttt tctgcattta tccgcgcggg
      121 cgttttaatt tttggcggac taattttact atcgcatcac tgtgcttgag gtggcggtgc
      181 gggcttccta aaaaaagatt tgttgtatat tacggccgca ataataagga atgcgccagg
      241 tccccaaatc caccacgcgc cccatgtgct cgcaaacaca taccgtccca cataaagagg
      301 attgcgccta acgtaaggag tatgagtggt ttcggatttt tgtgcgctcg gtagtcaaag
      361 ataaaaccta ctgcaccaag agcaatcagt ccgaatgcca accaacgcca tgctggtata
      421 agtgctccca agcccaagta ggtaaggagt gaggcagagc caatccaaca aagcgggcac
      481 acgcctattg cgcccgcgcc taagatcggc gccaaccttg tcagtagttt tttaccaaat
      541 gtttgcatat gattttccat agagctaatt agcgaatttg aacccacata tcgcctgcgt
      601 cgctactttt gaatagtttg ttttcgtgag tgagcgcata gacgatgttt gcgtcaatct
      661 tgctaaatgc gatgtgaagc acggcctcgc cgccaaaact ttcattgacc tgtgtccatg
      721 tttgtccact atcgccactt ttcctaagtc cgcccaatct ctcgctgaac gccaagagag
      781 tttttgagtt ttgaggatgt accgccacga ctgacacggc accaccctct aacgcgggcg
      841 atagcgcctg ccatgttgtt cctttgtctt ctgaaactat cacgccttga ccgcggggag
      901 tggcggcgta aactttgttt tcgttttccg aatctgcgga aagatacgcg gctacaatct
      961 ctcgatttat aattttccaa cttgcgccct ggtcctcact ccgttgaaga ttgcccgcgt
     1021 accaaccata gataaggttt ggatttaccg gactgaccgc cagcgcatgg aagtcaactg
     1081 ggccatttgc gccgtttgat attctcttcc atgtcgcccc gccgtcatcc gacctttgga
     1141 agccgagatt gccgccgcga gccggatggc cgctcgtgaa aaagatactt gggcctgtcg
     1201 gatgagccga aaaacccatg tagtcatctc tcgattttcc aactcggtag aggtctttct
     1261 cgctttcaag cagaaggaga ccgtagtgcg tggctatcca taatttgctc gggtctgcta
     1321 catcaactgc taggccgtgg ccatgggata ttgattgttc gggaataaga ttactttgag
     1381 tagagacgga gccactgttg ttctgacgca taagaattac tatcccacca gccaccacaa
     1441 aagcaaccag aatccaaaca tattttttag atgtttttgc catagtcatg attattttag
     1501 cacgaagtac aaactttgtt tatctttcgt gctgttaagt atatggcgat aagcataagc
     1561 agtgttattc cggcgacgaa ataaaaacga tactcaagta caaaaatcac accgccaaat
     1621 cctatcccga aaatagcgaa aagcggcgca agacacgaag cgcaaaacgc agtgccagcg
     1681 attcctgcaa acaaagtgcc aagccctcct gctgttgccg tgctcacgcc tccgattttt
     1741 cttttctgac gcataacata cacctgcatt gttatgaaaa gcgaggaaag agatgataaa
     1801 agaataagca ttacataatc acgggcggtg aaaatctcaa gctgggttgc taaagtattg
     1861 ccagcaaccg tccaaacagg aataaaaacg aaaagagcga agaaaacaaa aaatgaaaca
     1921 agaaaaagaa aaatgtaatt tgtttttttg aatggctcac gaataccgaa caaaaaaatg
     1981 tttaatgcgt ggctcatatt ttttagatac aacaacttaa cttgcttatg tctttggtaa
     2041 atgacaatgc ggcaattttt atcgcctctg aaagtgtcgg gaacatcggg agcgaattga
     2101 ccacatcgtc aattgtgttt ttgttcttga cgagcatcat cgcctcggcg ataagctcac
     2161 tcgcatgggc gccaagaata tgcacaccaa gaatttgttt tgtttgcgga tgaatcgcca
     2221 tttttatcat tccctgggtg cgatgtatga tgatcgcctt tggaacatcg gcaaacgaaa
     2281 cagtccggca ggtacaaaca cccatctgtt tcatctgctc atcttcggta aaaccgacac
     2341 cagcgagctg tgggtcggta aagatagcgt aagggacagt gttgtagtca ataaacaatt
     2401 gtgtgccttt gaggatattt tcagcggcaa gcgtgccctc gcgtccggcg gttgtctcaa
     2461 gtcgcacggg tgcaccggta acatcaccga ccgcaaaaat atttttgttg gaagtttgaa
     2521 aattctgatt gacgacaaca gcttgccgct tgtcaatttc aatgccagcg gtatcaagac
     2581 caagcccctg cgtgtttggc gttttgcctg ccgctaaaag tatctcgtcc gcgaccgttt
     2641 cttcttgcgc gccgtttata gtgtacaaga taactttttt accattctct ttacgcacgc
     2701 ttttcacctc aacatttgtt ttgatagtaa ttccttcttt tgcaagtatc tcggcgaggc
     2761 ggtcggtgag tgccttttca gagtgcggaa aaattgaatc accgcgttgt aaaatggtta
     2821 cttttgtgcc aaaacgcgca aacatttgag caaattccaa gccaagcggg cccgcgccga
     2881 taataaccaa ctctttcggc tgacgcggaa ggcgcaatgc ctcaatatgc gaaagaaatc
     2941 ccactcctct aattcctgca atctccggaa cattggcggt cgagcctgcg gcgataataa
     3001 atttcttgcc ccgtagcgag cttcgctctg ctacggggtt ccgctccgaa acaatttcta
     3061 tctcatctac accaacaaat tttgctttcc cctcgattgc cgttacatac tctaaatttt
     3121 tcaagacctt ctcgtatttc tcttgacgca atttttccac aagcgacagt tcgtcctgca
     3181 cgactttttg aaaatcaaaa tttttcactt caagctcaat gcccggagtg ccgtgatgtt
     3241 tggcgtggtg cagaatttcg ccagcgtaaa gaagcgtttt tgacgggaca cacccgacat
     3301 tgacacaagt gccgccgagc ggaagcccag cgttcacgag cgcagttttt gctttaagct
     3361 cgttagcgcg gatagcggct gcaaacgcgc ccgctccgcc accgataatt atcaagtcaa
     3421 aatttttcat gttatgtcgt aagacgctcc cttagcatac tagcgagagg cgtttgttta
     3481 agtttgtaat agaccgttcg gaattctcgg cggttttcaa ccaagcccgc tttttgcaac
     3541 tttttgagcg tatgagaaac agccgaaatg gaaacaccga taatttctga aatttcgccc
     3601 actgcgagtt ctttgtgccg acacaagaga tagcaaatct tgagccgcgt cgggtcgccc
     3661 accatgttaa attcctcggc acagcggaga atcgctttcc tatcgttcag ttcttttcta
     3721 attttttgaa tatctttcgt ttgcatatac attcaaatga taacaaatca aaacaaaaaa
     3781 cacaacaaga gttttatgca catggagtcc gccaaaaatt aaaacgcccg cgcggataaa
     3841 tgcagaaaaa gtcagccgag gttttgctaa atccccccca gaaaatagtt ttgacaaaac
     3901 cgagtccgca tttcgccccg ccacactgcc cgaatacttg gagggc
//
LOCUS       DATDSU010000031         3797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_365586, whole genome shotgun sequence.
ACCESSION   DATDSU010000031 DATDSU010000000
VERSION     DATDSU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365586"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VI981_00935"
     CDS             <1..695
                     /locus_tag="VI981_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478910.1"
                     /translation="RLVASLTKLMEAVVVAEHLDLRNSILVQSFMLDAYGATEGLIPG
                     QRPRIVELFYPLLVESSNDTAQVLSYFLGRIRTVELMQAKASALFMHNTVFTDTSGYD
                     PGNTSTAQDVFRLMRYIAFNRPTLLQITKGEMVRTFGEVRFTDLKNKNLFAQDPAFLG
                     GKTGYIVASQYTGAFLFEVPFGKETRTVAIVLLDSPSLEKGEQNLRQDTLNTLEWIER
                     TSSQLEISPKNL"
     gene            712..782
                     /locus_tag="VI981_00940"
     tRNA            712..782
                     /locus_tag="VI981_00940"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Lys,seq:ctt)
     gene            1092..1646
                     /locus_tag="VI981_00945"
     CDS             1092..1646
                     /locus_tag="VI981_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4478911.1"
                     /translation="MTRDGAYTILTDLIKNQNLVKHHLATEAVMRALARHFNEDEEKW
                     GLVGLLHDADYELTKDNPEKHTLVLEEKIGSSLEPDVMRAIKSHNAEYSGVEPQSKMD
                     WAIYTCDELTGLIIAAALIHPDKKLNSIDADFILRRFDQPAFARGADRTHITPCEEKL
                     GMPLKEFTELGLKAMQEISGGLGL"
     gene            1824..2591
                     /locus_tag="VI981_00950"
     CDS             1824..2591
                     /locus_tag="VI981_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY4478912.1"
                     /translation="MEILTWPIIGFIFFLVLFGLRIVNQYERGVVLTLGKYTGTRSPG
                     LTWILIGIQKMTKVDLRITTTDIPQQEVITKDNVPVGINAVVYFQVQNAEQAVLTIKD
                     YTLAVSQYAQAALRDVIGSIELDSLLSEREKNAEEIQKIVAQATGSWGILVTDIKIQD
                     IELPADMKRIMAKQAEAERERRAVIIKAEGEFAASERLAQAAGVLSSTPGGISMRTLQ
                     TIEKINPDPSKTVIFALPVEFFEGIKSLSKYFDGKQK"
     gene            complement(2595..3410)
                     /locus_tag="VI981_00955"
     CDS             complement(2595..3410)
                     /locus_tag="VI981_00955"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEY4478913.1"
                     /translation="MADKSTDRLNPKKRYLQVALNSTLEDAHEIINSLPKSDRIIIEA
                     GTPLIKRYGEDGIRQIRQWYEQHLSGQFLTPAISPSISVASLLFQAVFNAKSSLLITQ
                     NPVTIKSSTEVFPYIVADLKTMDRGETEVEIAARGGASAAIALGNAPIETLNSFVEKC
                     EEHGLDAMIDMMNVEFPLSVLRALKKIPAVVIIHRGVDEEKFNREKQIPLHEIRRIKG
                     NYNIMISIAGGDTLREVQRSFFNDADIVVIWKSVFQKTDDTVALIEGFLKEIK"
     gene            3441..>3797
                     /locus_tag="VI981_00960"
     CDS             3441..>3797
                     /locus_tag="VI981_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY4478914.1"
                     /translation="MLPVDSAQIIEVILCFGNGFELDIIKTSMDQLGPEYSVEVHDPK
                     TGMRGFLVIDNTTLGPGKGGIRMTPNVTEEEVARLARTMTFKNALADIPFGGAKGGIV
                     WPTRNASRSEAGGNGGS"
BASE COUNT         1066 a          855 c          883 g          993 t
ORIGIN      
        1 cacggctggt cgcctccctt accaagctca tggaggcggt cgtggtagcg gaacaccttg
       61 acctacggaa ttccattctc gttcaatcct ttatgctgga cgcatacggc gcaaccgaag
      121 gcctcattcc tggtcagaga ccccggatag tagaactttt ctatccgctt ctcgtggaat
      181 cctctaacga tactgcccaa gtcttgagct actttctcgg aaggatacgg acggtagaat
      241 tgatgcaggc gaaagcatca gctctcttta tgcacaatac cgtattcact gatacgagcg
      301 ggtatgaccc cgggaacacc tctaccgcac aagacgtctt ccgcctcatg cgctacattg
      361 ctttcaatag accgacgctc ttgcagatca ccaagggaga aatggtgaga acgtttggag
      421 aggtacggtt caccgaccta aaaaacaaaa acctcttcgc gcaagaccct gcgttccttg
      481 gaggtaaaac aggatatatt gtcgcctccc agtatacggg cgcatttctt tttgaggttc
      541 cttttgggaa agagacgcga actgttgcaa ttgtgctcct ggactcccct agcttagaga
      601 aaggagagca gaacctccga caggacacac tcaacacctt ggagtggatt gagcgaacct
      661 cgtctcagct tgaaatttcc ccaaaaaacc tctaggatga cagttgctca ggcccccgta
      721 gcttagcggc aaagcagttg gctcttaacc aattgacgag ggttcgattc cttccggggg
      781 cacaatcgta agcgtaaaat cttgacattc tgtatcattc agagtatgtt tcaactatgc
      841 cgcgtagttc aacccccact attgaaacca caaaggattt tatccttatt aagattccta
      901 gaaggatgtt ttctggggat gttgggcgtg gaaaactttc cggcttagaa cacggacttg
      961 cacaatctct acgggaagca gaacagggca agctgtacgg gccttttccc aatgctaagg
     1021 actttctccg cgctctcaaa aagccaggaa gatgaatgct tccttcactc cgcatttcca
     1081 gaggacaaaa tatgacaaga gatggggcct atacaattct taccgaccta attaagaacc
     1141 aaaacctggt taagcatcac ctggccacgg aagctgtgat gagggccttg gcaagacatt
     1201 ttaatgaaga cgaagaaaaa tggggtctgg tcggcttgct ccacgatgcc gattatgaat
     1261 taaccaaaga caatccggaa aagcacacgc ttgttttgga agaaaagatt ggcagttcat
     1321 tggaacctga cgtcatgcgt gccataaaga gccacaatgc ggagtattcg ggagtggagc
     1381 cgcagtcaaa aatggactgg gcgatttata cctgtgatga actaacgggt ctgataatcg
     1441 ccgccgcgct tattcatcca gacaagaaac tgaactcaat agatgccgat tttattcttc
     1501 gaagattcga ccaaccggct tttgcgagag gcgccgatag aacgcacata actccttgtg
     1561 aagagaaatt agggatgccc ttgaaagaat ttaccgaatt gggcctcaag gcaatgcaag
     1621 aaatttctgg cggcttagga ctataatgga caggttcggc ccggaacacg tagtaaaggt
     1681 atacgatcct gaaacaggaa tgcggggctt tttgtatttg ctgatttgct ccggaacatg
     1741 atatactaaa cacaagcaag aaatggaaat ccacgagatt tgacctattg cgctttccag
     1801 acttgtgcta taataaaaac attatggaaa tacttacgtg gccgataatt ggttttatat
     1861 tcttcctcgt cctctttgga ttaagaatag ttaatcaata tgagcgcgga gttgtgctta
     1921 ctcttggtaa atacacggga acgcgttcac ccggcttgac gtggattttg attggaattc
     1981 agaaaatgac caaagttgat ttacgtatca ccaccaccga cataccacag caagaagtta
     2041 taacaaaaga caatgttcct gtgggcatca acgcagtagt atacttccaa gttcaaaatg
     2101 ccgagcaggc tgttttgact ataaaagatt atacgcttgc ggtttcccaa tacgcacaag
     2161 cggcgcttcg agatgttatc ggcagtattg aacttgactc gctcttgtca gaaagagaaa
     2221 aaaatgccga agaaatccaa aagattgtcg cccaagcaac agggtcttgg ggtattttgg
     2281 ttactgatat taaaatccaa gacatagaac ttccagcaga catgaagcgc ataatggcaa
     2341 agcaagcgga ggcggaacgt gaacgccgag cagttatcat caaggcggaa ggagaatttg
     2401 cagcgtcaga gcgacttgct caagcggccg gtgtcttgag cagtacgccg ggtggtattt
     2461 caatgcgaac tctgcaaaca atagaaaaaa taaatcccga tccttccaag acggttatct
     2521 ttgctttgcc tgttgagttt tttgaaggaa taaaaagtct ctcgaaatat tttgacggaa
     2581 agcaaaagta gttgctattt gatttctttc aaaaatcctt cgataagggc gacggtgtca
     2641 tccgtctttt gaaatactga cttccaaatc acaacaatat ccgcgtcatt aaaaaatgac
     2701 cgttggactt cgcgaagtgt gtcgcctccg gcaatagaga tcataatatt gtagttgcct
     2761 tttatacggc ggatttcatg taggggtatc tgcttttctc gattaaattt ctcttcatct
     2821 acacctcggt gaataataac cacagccgga atttttttga gtgcgcgtaa taccgataaa
     2881 ggaaattcaa cattcatcat atcaatcatt gcgtccagtc cgtgttcctc acatttttca
     2941 acaaacgaat tgagggtttc tattggcgca tttcctaagg caatagccgc gctcgctccg
     3001 cctcgtgcgg ctatttcaac ctctgtctca cccctatcca ttgtttttaa gtcagcaacg
     3061 atgtagggga atacttctgt gctacttttt atcgtaaccg gattttgtgt tattagaagg
     3121 gaagattttg cattaaaaac ggcttgaaaa agtaatgagg ctacagaaat tgacggcgag
     3181 atggccggtg ttaaaaattg cccagacaga tgttgctcgt accattgacg aatttgccgt
     3241 attccatcct caccatatct tttgataagg ggcgttccgg cttcaataat aattcgatcg
     3301 cttttcggca agctgttgat tatttcgtgg gcgtcttcaa gcgtgctatt taacgccact
     3361 tgcaagtagc gctttttggg atttagccta tctgttgatt tgtccgccat agataatctc
     3421 attatactag attttgacga atgttaccag ttgattcggc gcaaatcatt gaagttatcc
     3481 tctgttttgg aaatgggttc gagcttgata taataaaaac atccatggat caacttggac
     3541 cagaatattc agtagaagta catgacccaa aaacaggaat gcggggcttt ttggtcattg
     3601 acaataccac tctgggcccc ggaaagggcg gcattcgtat gaccccaaac gtcaccgagg
     3661 aagaggtggc tcgccttgcc agaaccatga cattcaaaaa cgccttagca gacattccat
     3721 tcgggggagc aaaggggggc atcgtatggc ctacccgcaa cgcttcgcgt agcgaggcgg
     3781 gcgggaatgg ggggtct
//
LOCUS       DATDSU010000032         5280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1703721, whole genome shotgun sequence.
ACCESSION   DATDSU010000032 DATDSU010000000
VERSION     DATDSU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703721"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VI981_00965"
     CDS             <1..1486
                     /locus_tag="VI981_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY4478915.1"
                     /translation="GNFDVGANSGGGSGRFDGLIDDVRVYNRALSATEITQLYNVGQA
                     KVAKTPSTGSGQASNPLSLGLVGHWTFDGKDTPGRALDVSGQANHGNLVNMSTSTSRA
                     PGKIGQGLRFDGVNDAINTGSDMIGTGEDSISAWINPRSFALSGNDSRIVTNDKTILT
                     MRSSNSTIGISSNGIALVEAASSAIVLNRWTHVTVTRNSSGTANLYVNGILSGTANQN
                     SGTPSGGSTNVFIGNSSLLTSRPFDGIIDDVRVYNRALSATEITQLYNVGQAKIAKTQ
                     SPAPCGSGAPLSCGLVGHWTFDGKDTPGRALDVSGQGNHGNLVAMSTSTSRVPGKIGQ
                     GLKFDGVNDYVKISSDIIGTTALTITAWIKPTASTLSVGTGRIVTNGNVTIFKLASSN
                     RLSFTSNNSNTAVSATNALSGTANTWQFISVTRNESGTANIYLNGTLTGTANQNSGSP
                     IVGTDFEIGAQLAASFFDGSIDDVRVYNRALSPTEVQRLYQLGR"
     gene            1691..1906
                     /locus_tag="VI981_00970"
     CDS             1691..1906
                     /locus_tag="VI981_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478916.1"
                     /translation="MNQITLTKNITKGDELVVISRKEYDRVLVAYEKLKWHQKEREAH
                     DDILKGRLSKGYTTKKELRAALDKLKK"
     gene            2328..2765
                     /locus_tag="VI981_00975"
     CDS             2328..2765
                     /locus_tag="VI981_00975"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY4478917.1"
                     /translation="MIEIGEYVRYSAYMDIEAVQSKIAGIAQKHGLDFVVLFGSQATG
                     KTHPKSDIDIGIISKAPFDINRLTIDFYYDVFKREDVEVVDLSSVSSTLMRAVVRDGK
                     LLYERTDGLFMQWKLYAIKIWMETAWMRKVRDRKLVEWAERHR"
     gene            2796..3185
                     /locus_tag="VI981_00980"
     CDS             2796..3185
                     /locus_tag="VI981_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEY4478918.1"
                     /translation="MDTLRAYYEELKPWISFPSGEILANNEKIRAIERLFQLLVDEAV
                     DINTLIIADSTQESPENYRSTFYGLSELGLLDHTFADTISFSAKLRNQIVHDYDKVQR
                     SVMIEDIKKFILLYEKYIATLIEKFVA"
     gene            3649..4059
                     /locus_tag="VI981_00985"
     CDS             3649..4059
                     /locus_tag="VI981_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY4478919.1"
                     /translation="MQKDFDQWNEKKKSLHGSVFTDFVHTREVWWCAVGVNVGVEADG
                     KRENFERPVLVIRKFSTEAVLMIPLTSRVKKDNPYHVIFSHEGREFSAVISQVRLIST
                     KRLLRKMYQMDSSIFTRIISSIVTIIQKNRPPLA"
     gene            4094..4612
                     /locus_tag="VI981_00990"
     CDS             4094..4612
                     /locus_tag="VI981_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478920.1"
                     /translation="MLTNHKTKSSFRYAHLLAIIFASFFAFLILFLPAQGVHAQTSPV
                     IPAPASSETSSRGRQAGIQESSLASIVSSLQSILLSLKQLITASYHKLPSVSPTTQLA
                     QISGTDSGLVAHYTFDEGSGTTAGDSSGNGNTGTLTPTTGGPTWTSGKVGNGTLQFDG
                     VYRWSLDGIVSF"
     gene            4642..>5280
                     /locus_tag="VI981_00995"
     CDS             4642..>5280
                     /locus_tag="VI981_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HEY4478921.1"
                     /translation="MASQASKRNELIREFLFKNKIGSSSGIHSFILSRDEDISLVTVK
                     RALSLLEKEGVIAEQGTGPATAYELTAQGMITTSIDARTYCAIEPDRRYGNERYNFEL
                     FAHLSDEIFSPAELDILQNATKVYQSRRTSLSQTLHKKELERFIVELSWKSSKIEGNT
                     YSLLDTERLIEKGIEAPGKSKEEALMVLNHKKAFSYIYEHADEFKTLTKTNLS"
BASE COUNT         1520 a         1140 c         1236 g         1384 t
ORIGIN      
        1 gggtaatttt gatgtgggtg caaattctgg gggtggatcg ggtcgttttg acggcctgat
       61 cgacgacgtc cgcgtctaca accgcgcatt gtcggcgaca gagatcaccc aactctacaa
      121 cgtgggtcaa gctaaagtag ccaagacccc ttcgacaggc tcagggcaag ccagcaaccc
      181 gctctccctt ggactcgtcg gccactggac ttttgacggc aaggacacac ctggcagagc
      241 gcttgatgta tcaggccaag cgaaccacgg caacctagtc aacatgtcca cctccacctc
      301 acgtgctccc ggcaagattg ggcaaggatt aaggtttgac ggggtgaatg atgctataaa
      361 tactggcagt gatatgatag gaacaggaga ggatagtatt tctgcatgga taaatccgag
      421 aagttttgca ttgagtggaa atgatagtcg tattgttact aatgacaaga caatccttac
      481 tatgcgtagc agtaactcaa ctattggtat atctagtaat ggtatagcac tcgtagaggc
      541 ggcaagtagt gcgattgttc taaataggtg gacacacgtt actgttacta gaaattcatc
      601 aggtacagct aacttatatg taaatggtat tttgagtgga acagcaaacc aaaacagtgg
      661 taccccatca ggaggctcaa caaatgtatt tattggtaat agtagcttac tgacatcacg
      721 accatttgat ggaataatag acgacgtccg cgtctacaac cgcgcgctgt cggcgacaga
      781 gatcacccaa ctctacaacg tgggtcaagc aaaaatagcc aagactcaaa gccccgctcc
      841 ctgcgggagc ggggctcctc tctcctgcgg cctagtaggt cactggacct ttgatggcaa
      901 agacacacca ggaagggcac ttgatgtgtc aggccaaggc aaccatggca accttgttgc
      961 catgtcaacc tccacttcac gagttccggg caagattggt caggggctca agtttgacgg
     1021 ggtgaatgat tatgtcaaaa taagcagtga tattatcggc actacagcgc tcacgattac
     1081 agcatggata aaaccgacag cttcaacact ctcagttggc acaggcagga ttgtaacgaa
     1141 tgggaatgtc acaattttta agttagcttc aagtaatcgt ctctcgttta ctagtaataa
     1201 ttcaaacact gctgtctccg ccaccaacgc tctctctggc acagcaaata catggcagtt
     1261 catatctgta actagaaatg aatcaggtac ggcaaacatt tatctcaacg gtacacttac
     1321 agggacagcc aatcagaata gcggttctcc gattgttggt actgattttg aaattggggc
     1381 acagcttgcg gcatcatttt ttgacggctc catcgacgac gtccgcgtct acaacagggc
     1441 attatcacca acagaggttc agcgtttata ccaattaggg aggtgatgtc ataatctgca
     1501 tctacaaccc cgacgctaag aaggtcgggg caggctgcgc gctgtcggcg ggggaggtgt
     1561 tgcagttgta ccagctcgga cgataagtct tatccgccct attcgatcta tatgtcgaat
     1621 aggacggaga ggttgaataa gacggatgcg ttccgttgat ttttctaaaa tagtttagta
     1681 tatttgaact atgaaccaaa taacgcttac aaaaaatatc acgaagggcg atgagcttgt
     1741 tgttatatcc cgaaaggagt atgacagggt tttagtcgcc tacgaaaaac tgaagtggca
     1801 tcagaaggag cgggaggcgc acgacgacat ccttaaggga agattgagca aggggtacac
     1861 gacaaaaaaa gaattgcgag ccgctctcga taagctgaag aaataatatg gaaattcggt
     1921 ttacgcgttc gttcgaaagg gattatcgcg aacttccgaa agccttgcag tcggcagtag
     1981 ataaaaaatt acttttgttt cttggaaatt caaaacatcc atctttgcgg gtaaaaaaga
     2041 tggagggata tgcgaatata tgggaggggc gagtaagtaa gggatgccgg ttcacttttt
     2101 gtattattga tgcaacgtat gttattcgcc gtgttgggac acatgacgtt ttaagaaagc
     2161 cctaatggaa aggctccctc gacgacgtcc gcatctacaa ccgcgcattg tctgcggggg
     2221 agatacaatt gttatacaac ttgggaagat gatgaactag aaaatagtaa gtagaaagta
     2281 gaaaatagaa gcgagaatta acaaattctc aattttgcaa tttataaatg attgaaatcg
     2341 gggaatatgt gcggtatagt gcatatatgg atatagaggc agtacaatcc aagattgcgg
     2401 gtattgcgca gaaacatggg cttgacttcg tggttctttt tggttcgcag gcaacgggca
     2461 agacacaccc taaaagcgat attgacatcg gtattattag caaggcgcct ttcgatatca
     2521 atcgtttgac tattgatttc tactacgacg tttttaagag ggaagacgtt gaagttgtcg
     2581 atctttcttc cgtttcgtcc actcttatgc gtgccgtagt gcgtgatggc aagcttctat
     2641 atgaacgtac agacgggctt tttatgcagt ggaaactgta tgcgataaaa atctggatgg
     2701 aaacggcatg gatgagaaaa gtgagagacc gcaagttggt agagtgggcc gagcggcata
     2761 gataaacgta tgcataacga cgaaataaag aaaaaattga tacacttcgt gcatattacg
     2821 aagaattaaa accgtggata tcttttcctt ccggcgaaat tctggcgaac aatgagaaaa
     2881 tccgcgcgat tgagcggctg tttcagcttc ttgtggatga ggcggtagat ataaatacgc
     2941 ttattattgc tgacagtaca caagagagtc cagaaaatta tcgctcgaca ttttatggac
     3001 tcagcgagct tggtctcctc gaccatacgt tcgcagatac aatttctttt tctgcaaaat
     3061 tgagaaatca gattgtgcat gattatgaca aagttcaacg gagtgttatg atcgaagata
     3121 ttaagaaatt tattttgctg tatgaaaaat acattgccac tcttattgaa aagtttgtgg
     3181 cataagtcaa ccctctggta tgtacatttt tacggcatct tttttgcgct ttttctcgtt
     3241 tcttgcttct tcataattcc cgtatccatt tccgcccaaa ccagcaatgt gtcaggtatc
     3301 acgtatcagg tgtcaagcac agaccaagac acctcccggc tcgcctcaat cgtctcttcc
     3361 ctccaatctc tcatcggaac tcttcaatcg cttttttcta atctctaatc tcctatctct
     3421 aatctcggat atcaaaccca actcgcccaa atctctggcg caggatcccc gacgccagga
     3481 aggtcggggc aggccagtct tgttgctcat tatacgtttg acgaaggcag tggaataaca
     3541 gcaggggatt caagtgggaa tgggaataca ggcaccttag ctccgccagc tggcggaggc
     3601 cctacatggc aaattgcggt tcagaagtta tggtaaacta aagaagttat gcaaaaagat
     3661 tttgaccaat ggaatgaaaa gaaaaaatct cttcacggaa gcgtttttac tgactttgtt
     3721 catactcgag aagtgtggtg gtgtgcggtt ggtgtgaacg taggcgtcga agccgatgga
     3781 aagcgcgaga atttcgaacg gccggttctt gtaattcgca agtttagcac agaagcagtt
     3841 cttatgattc cgcttacttc gcgtgtaaaa aaagataatc catatcatgt tatattttca
     3901 cacgaaggac gggaattctc tgctgtaatc tctcaagtgc gtttaataag caccaagcgg
     3961 ctattgcgga aaatgtatca gatggatagt tctatattta ctcgaattat ttcttcgata
     4021 gttacaataa tacaaaaaaa tcgaccccca cttgcgtagg ggcctcggag gccgcatggc
     4081 cattagtgac tatatgctaa caaatcacaa aacaaagtca agcttccgtt atgcacatct
     4141 tttagccatt atttttgctt cttttttcgc ctttttgatt ctctttttgc ccgcgcaagg
     4201 agttcatgcc caaacttccc ctgtcattcc cgcgcccgcc tcgtcggaga cgtcgagtcg
     4261 aggcaggcaa gcgggaatcc aggaatcctc tcttgcttct atagtttctt ccctccaatc
     4321 aattctctta tctctaaaac aactaattac cgcgagttac cataaattac catcagtttc
     4381 tcctactacc caactcgccc aaatctctgg cacagacagc ggtcttgtgg ctcactacac
     4441 ttttgacgaa ggcagtggca ccacggcagg ggattcgagt gggaatggga atacggggac
     4501 gctaaccccg accactggtg gcccgacatg gacgagtggc aaggtaggga atggtaccct
     4561 ccagtttgat ggggtgtata gatggagctt agacggaatc gtatcatttt gatacaatag
     4621 tacacatata ttgatacgat tatggcatct caagcaagta aaagaaatga acttattagg
     4681 gaatttttgt tcaaaaacaa aatcggttcc tcttcgggta ttcattcctt tattctctcg
     4741 cgagacgaag atatatctct tgtcactgta aaacgagcgc tttctcttct cgaaaaggag
     4801 ggagtgatcg ccgaacaggg cacggggcca gccaccgcat acgaacttac cgcacaaggg
     4861 atgatcacta catccattga tgcccgaaca tattgtgcaa tcgagcccga tcgacgttac
     4921 ggaaacgagc gctataattt tgaattattt gctcatctgt cggatgaaat cttttctcct
     4981 gcagaattgg atattcttca aaatgccaca aaggtatatc agagccggag gacgtctctt
     5041 tcccaaacgc tccacaaaaa agaattagag cggtttattg ttgaactctc gtggaaatcg
     5101 tcaaaaatcg aaggcaatac atactcattg ctcgacacag aacgacttat cgaaaagggg
     5161 attgaagctc cgggcaagag caaagaagaa gccctcatgg tgttgaatca caaaaaagcg
     5221 ttttcataca tatacgaaca tgctgatgaa ttcaaaactc ttacgaaaac caatctttcc
//
LOCUS       DATDSU010000033         7810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2200431, whole genome shotgun sequence.
ACCESSION   DATDSU010000033 DATDSU010000000
VERSION     DATDSU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200431"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..223)
                     /locus_tag="VI981_01000"
     tRNA            complement(141..223)
                     /locus_tag="VI981_01000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Ser,seq:tga)
     gene            complement(318..536)
                     /locus_tag="VI981_01005"
     CDS             complement(318..536)
                     /locus_tag="VI981_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HEY4478922.1"
                     /translation="MQFGDIFIPLWLMTIVFIWVMIWKGIALWKAARNEHAWWFAALL
                     VVNTLGILEIIYILWDIRARKKISEPEK"
     gene            687..1127
                     /locus_tag="VI981_01010"
     CDS             687..1127
                     /locus_tag="VI981_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HEY4478923.1"
                     /translation="MKYMGIDYGSKRVGIAISDERNEFAMPKAVYPNDERLLQTVLDF
                     VAHNDVGKIVMGESTDFQGRENKIMREVHEFKKNLERESGKSVFLEPEFFTSQEAERT
                     GNRRPDHHAGERPSRGETKNEMLDASAAALILKSFMDKKKNDQF"
     gene            1152..1544
                     /locus_tag="VI981_01015"
     CDS             1152..1544
                     /locus_tag="VI981_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEY4478924.1"
                     /translation="MRIMISFDDFKKMEITMGKILSAEKIADTDKLLKLKVHFGTKLE
                     CVPQEQLQKSELNECDDVRQVVSGIVLHFPDPEKLVGLTCAFVTNLEPRNIKGLVSEA
                     MILALGGDGEPFSLLVPNAEVSPGTKAR"
     gene            1546..2157
                     /locus_tag="VI981_01020"
     CDS             1546..2157
                     /locus_tag="VI981_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015167142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY4478925.1"
                     /translation="MHFLSYLEPKFLIETAGLLGVFFIIFAESGLFFGFFLPGDSLLF
                     TAGFLASQGYFSISILVGGAVLAAIVGDTAGYAFGRKVGPKIFSRDDSLFFHKKHVVR
                     AQRFYANHGNKTIILARFIPVVRTFAPILAGVGAMSYGTFLKYNCIGGFLWAAGIPLV
                     GYLLGGAIPGVDQYLLPIIIMIVVLSVLPGAFHFYRSRKTEDL"
     gene            2229..3332
                     /gene="pilM"
                     /locus_tag="VI981_01025"
     CDS             2229..3332
                     /gene="pilM"
                     /locus_tag="VI981_01025"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY4478926.1"
                     /translation="MKGRNITTRFFKDFFPPPQYLRFPSVGVDISNQSLRIIELVRRH
                     GRTEIGKWGEHKIPAGIIYRDAITDESELKTTLEEVKKKYNLKFIHVSLPEDKAYVFN
                     TTIARIPVKEIRGSLELQLEENVPLRPDEVEFDFTIIGEDTAHDSLRLGVAVFPKVFI
                     DRLTALLESVGLTALSYRVSADAIAAAVTSKTDLSANMIVNFGEERTGIYIAIKGAAV
                     FSSTLNFGAAALLSGIQKHFGVTHEEALKIRRGANNPYNKSSMQLFYSVINPISALKD
                     EINRILLYWKTHKDKQGSISAPVSSIILCGSNSNMPAMDEYLSLSLKLPTHSAEVWKN
                     ALDLDDYIPPITHAQSLDYAAAVGLALGEETLL"
     gene            3329..3862
                     /locus_tag="VI981_01030"
     CDS             3329..3862
                     /locus_tag="VI981_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478927.1"
                     /translation="MINLLPVQRKKSLKTEYVLRLVITILVFFGILGYAGIAMLIPSY
                     VYSKFRLKTIKVESEQIRKKNEGSQNSEISAILSQTSKKLELVKPNERQYLSHLLIRV
                     IGLRGSGISFSTISVSGAGNERTLILSGLAKTRTELIEFYKRFDNDKSFSKVNLPISS
                     YSKDKDIEFNVTFTTKI"
     gene            3859..4386
                     /locus_tag="VI981_01035"
     CDS             3859..4386
                     /locus_tag="VI981_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478928.1"
                     /translation="MKWFKHTITVLFVAFLFAVLAFVGYYGLYQLTNKSNAEIGVALT
                     QMAFEAKIDDRLTSLSSIVAESQAERERLDMVFVEKNGLDFITKIEEMATHAGVTLAK
                     DLDTTALPPNYAEIAETLQANMEIKGPWNNVFYFVELVESMPYKIDIDSMAFIYERGN
                     MWKANMRIRTLLLKK"
     gene            4396..4710
                     /locus_tag="VI981_01040"
     CDS             4396..4710
                     /locus_tag="VI981_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478929.1"
                     /translation="MKFSFLQRIKKTGSPTSLGINVHRDWVVLFLTTVVLALCVLTFS
                     YNFFRKIERGDAFLVNEEQPVGLENLDRKKLGDTVNFYERHREQFSNLEKNRPPYKDP
                     SL"
     gene            4753..4824
                     /locus_tag="VI981_01045"
     tRNA            4753..4824
                     /locus_tag="VI981_01045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4786..4788,aa:Arg,seq:tct)
     gene            5051..5263
                     /locus_tag="VI981_01050"
     CDS             5051..5263
                     /locus_tag="VI981_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478930.1"
                     /translation="MNRKHLKCTLWKLLWTLGVLSLIAAWVSSQSDPVLEFEWSFWFW
                     NALVFGVLAVPIKLDCHSCDVCGIGK"
     gene            complement(5305..6198)
                     /locus_tag="VI981_01055"
     CDS             complement(5305..6198)
                     /locus_tag="VI981_01055"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4478931.1"
                     /translation="MSVGEKIIPDHREETSTALGFFVRVRAAGMFRHQTKRPRRGRGN
                     LLAAASELSVTTEKNCFRFFKTCARRSGVDTVVRPASQGREIVRTSVGKILLIKITQF
                     MSKKRVIIVHGWDGHPEEGWFPWLKNELEKLGFEVLIPQMPDAANPRIYNWVPALAKS
                     VGTADTNTYFVGHSMGCQTIIRYLESLPEKKKVGGAVFIAGFLKRLTGLEDDTAVRET
                     DRHWLTTPIDLDKVKKQIGKTIAVFSDNDPWVPLDNRDEFENKLGSKIFIEHGKGHFS
                     GIADSCFELPIVLDSILEIVE"
     gene            complement(6302..6374)
                     /locus_tag="VI981_01060"
     tRNA            complement(6302..6374)
                     /locus_tag="VI981_01060"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6339..6341),aa:Val,seq:tac)
     gene            complement(6409..7410)
                     /locus_tag="VI981_01065"
     CDS             complement(6409..7410)
                     /locus_tag="VI981_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEY4478932.1"
                     /translation="MITNEAKERCRILAFWQKHGTEATEEAFKVKKRTLFIWQQLLKE
                     GGGKLEALNPKKKTPKNKRKRVWDAKLIEEIKRLRNKYPNLGADKIYPLLLDFADVEG
                     IAKRPGSSTIERLIVDLGGLRTSPQKVSHFGKIKKANRQKVLRKPKDLVPTYPGHVLA
                     LDTIEKQRHGRRMYVLVAIDIFTRTVFAIATKSHSSKTFAHFFFLVMRMFPYEIRNVL
                     TDNGSEFKKCLSELLSQNHITHYHTYPKTPKMNAHCESFNGTIQEEFVDYHINLLFDN
                     TTLFNEKLSEYLTFYNTRRVHYAFKNKMTPLEVLTRSTYYVNQLPAECKNGWGYSKI"
BASE COUNT         2185 a         1628 c         1754 g         2243 t
ORIGIN      
        1 cgatgggtac ctggagttta aaaaggaagt ctgcgaacta atcgtctgat ttccctcaat
       61 tcaaccgaaa agaccggacg atatcgtctg gtcttttttc tacccaaaac ctaatttcca
      121 tgtgttcgat aaaaacgatg cggaaggggt gggactcgaa cccacatgac ctttcggtct
      181 ccagttttca agactggtgg aataaccatt atccgacccc tccttaaaag aatgttttga
      241 taatcggtgc gcggcgacaa taagtcagaa attttctata aatgctcaaa agactttagt
      301 ttgaaatttg agatcggtta tttttccggt tccgaaattt tcttccgcgc ccgaatatcc
      361 cacaatatat aaattatctc taagattccg agcgtattca cgactaaaag tgcggcaaac
      421 caccatgcat gctcatttcg agcggctttc caaagagcga tacctttcca gatcatgacc
      481 cagatgaaca caatggtcat aagccacaaa gggataaaaa tgtcaccaaa ttgcatagga
      541 agcataataa caaagccggt aggcattgca acatgatata catcgtgcca atatacgaat
      601 gagtaccaat gatcccaacg atgttagtaa gaaatgcttg tttttgcgcc agttcgtata
      661 attggttaag ttagtatact tgtagtatga aatacatggg aattgattat ggatcgaagc
      721 gtgtcggcat agcgatatcc gacgagcgga atgaatttgc aatgcctaaa gcggtctatc
      781 ccaacgacga aagactttta caaacagtcc tcgattttgt tgcccacaat gacgtaggaa
      841 aaattgttat gggagagtct actgattttc aggggaggga aaacaagatt atgcgtgaag
      901 ttcatgagtt caaaaaaaat ttagagcgcg aatctggcaa aagtgttttc ttagaaccag
      961 aatttttcac ttcgcaagaa gcggaacgaa cgggcaaccg aaggccggac catcatgccg
     1021 gcgaacgacc atcacgagga gaaacaaaaa atgagatgct ggatgcctct gcagccgcac
     1081 ttattttaaa gagttttatg gacaagaaaa aaaatgatca attttaagtt aagaaccctt
     1141 acaaataggg catgcgaatt atgatttctt ttgatgattt taaaaaaatg gagataacga
     1201 tgggcaaaat tctttcggcg gaaaaaatcg ccgacacgga taaactgctc aagctcaagg
     1261 ttcattttgg caccaaactt gaatgtgtgc cgcaggaaca acttcagaag tcggaattaa
     1321 acgaatgtga tgatgtgagg caagtcgtgt caggaatcgt acttcatttc ccggatccgg
     1381 aaaaattagt tggtcttacg tgcgcctttg taactaatct cgaacctcga aatattaaag
     1441 gtctcgtgag tgaggctatg atccttgcgc tcggtggaga tggtgagccg ttttcattgc
     1501 ttgttccgaa cgctgaagta agccctggga cgaaagctcg ataatatgca ttttttatcg
     1561 tatctcgaac cgaaatttct gattgaaacc gccggccttc tcggagtttt ttttatcatt
     1621 tttgcagagt ccggtctctt tttcggtttt tttctccccg gagattctct gctctttaca
     1681 gccggatttc tcgcttcgca gggatacttt agcatctcga ttcttgtcgg cggggcagtg
     1741 ctcgctgcca tagtgggcga tactgcaggt tatgccttcg gtaggaaagt tggacctaaa
     1801 attttctcac gtgacgactc gctttttttt cacaagaagc atgtagtgcg ggcacagcgc
     1861 ttttacgcaa accatggaaa caaaacgatt attttggcgc gttttatccc tgttgtgagg
     1921 acttttgcgc ccattcttgc aggagtcggc gcgatgagct acggcacgtt tctcaaatac
     1981 aactgcatcg gcggattttt gtgggcggcc ggtattccgc tcgttggcta tttattgggc
     2041 ggcgcgatcc cgggcgtcga tcaatatttg ttgccgatta ttatcatgat agttgtgctg
     2101 tcggtgttgc cgggagcgtt ccatttttac cgttcaagaa agacggaaga cctttaggtt
     2161 tccctagaaa aagctcggaa ttgcctgtat aattctaggt tacaaggtca cagtggccac
     2221 ttttcgcgat gaaagggagg aacatcacaa cacgtttttt taaagatttt ttcccaccgc
     2281 cccaatattt gcggtttcct tccgtgggag tggatatttc aaatcagtca cttcgtatca
     2341 tagagcttgt tcggcgtcac gggcgaaccg aaatcggaaa atggggagag cacaagatac
     2401 ccgcaggaat tatttatcgc gacgcgatca ctgatgaaag cgagctcaag actactttag
     2461 aggaggtcaa gaaaaaatat aatctgaaat tcatacacgt ctctttacct gaagacaaag
     2521 catatgtgtt caatacaaca atcgcacgaa ttccggtaaa agagatacga ggcagcctgg
     2581 aacttcaact tgaagaaaat gttccattgc gcccagatga agttgaattt gacttcacaa
     2641 taatcggtga agatacggca cacgattcgc tgcgtctcgg ggttgctgtc tttccgaaag
     2701 tttttataga ccgtcttaca gcgctcctcg aaagcgttgg acttacggca ctttcatatc
     2761 gcgtcagtgc cgacgcaata gctgcagctg tgacgagtaa aactgacctc tcggccaaca
     2821 tgattgtaaa tttcggcgaa gaacgaacag gtatttacat tgctatcaag ggcgctgctg
     2881 tattttcttc caccctaaat ttcggagccg cagcgttatt gtcaggaatt caaaaacact
     2941 tcggtgttac tcacgaagaa gcactgaaaa ttcgaagggg cgcaaacaat ccctataata
     3001 agtcgagtat gcagcttttc tattcagtca tcaatccgat ttcagcgctc aaggatgaga
     3061 tcaaccgcat attgctatat tggaaaacgc ataaagataa gcaggggagt ataagtgcgc
     3121 ccgtctcgag catcatcctt tgtggtagta acagcaacat gcccgccatg gatgaatatc
     3181 tctctttgtc tcttaaactt ccgactcatt cagccgaggt ttggaagaat gcgcttgatt
     3241 tagatgacta cattccacct attactcatg cgcagtcgct tgattatgcc gcggcagttg
     3301 gacttgcgct tggtgaagaa acgctcctat gattaatctt ttaccggtac aaagaaaaaa
     3361 aagtctgaaa accgaatatg tactaaggct tgttattacg atactcgttt ttttcggtat
     3421 cttgggttat gcaggcatag ccatgcttat tccatcatac gtgtattcta agtttcgact
     3481 gaagacaatc aaagttgaaa gtgaacagat ccgaaaaaaa aatgagggta gtcaaaatag
     3541 tgagatatcg gctattttga gccaaacttc aaaaaaattg gaattggtaa aaccgaatga
     3601 gagacaatac ctgtcgcatc ttctcatacg agtgatcgga cttagaggtt ctggcataag
     3661 tttcagcacc atatcggtat cgggagcggg aaatgaacga acgcttatat taagcggcct
     3721 tgcgaagacg agaaccgagc tcattgagtt ctataaacgc tttgataacg ataagtcatt
     3781 ttcaaaagtc aatttgccaa tttcaagtta ctcgaaagac aaagacatag agttcaatgt
     3841 tacctttacg acaaaaatat gaagtggttc aaacacacta taacagtttt gttcgtcgcg
     3901 tttttattcg ccgtcctcgc gtttgtcgga tattacggtt tgtaccagct gacaaacaaa
     3961 agtaatgctg aaatcggagt ggcccttacg caaatggcat ttgaagcgaa gatagatgat
     4021 aggcttactt cgctctcatc aatcgttgcg gagagccagg cagaacggga gaggctcgat
     4081 atggtatttg tcgaaaaaaa cggtcttgat ttcataacga aaattgaaga aatggccact
     4141 catgcggggg ttacccttgc taaagatctg gacaccacag cgcttccacc aaactatgcg
     4201 gagattgccg agacgcttca agcaaatatg gaaataaaag gaccgtggaa taatgttttt
     4261 tattttgtcg agcttgtcga aagtatgccg tataaaattg atattgattc aatggcattc
     4321 atctacgaaa gagggaacat gtggaaagct aatatgagga tccgcacgct actattaaaa
     4381 aaataatgta agtacatgaa attttctttt ttgcagagaa taaaaaagac cggcagtcct
     4441 acaagtcttg gaatcaatgt acatcgagac tgggtcgttc tttttttgac gacagtcgta
     4501 ctcgctcttt gcgtgctcac ttttagttac aattttttcc ggaaaattga gcgaggcgac
     4561 gccttcctcg ttaacgaaga acagccagtc ggtttggaaa atctggaccg gaaaaaactt
     4621 ggtgatacgg tgaactttta tgaaaggcac cgagaacagt tttcaaacct tgaaaaaaac
     4681 cgcccaccgt ataaagaccc gtcgctttag aaaattggga ttaggatgcc tgttctggta
     4741 tattctcatt acccccccgt agcttaatgg ataaagtagg gctcttctaa ggccttgatg
     4801 taggtccgat tcctgccggg gggacaaaat tctcattttg gcaccacgag caagttgcct
     4861 gcgcagcttg cgtgtgggaa ttgaaatact tttcgttatc gatccgtcag gcgatggaga
     4921 gatcgaaaag tccccgcaac cgtagaacga gattcctgca aaaaaggggg gtatagcacg
     4981 ctcgcgaatc tgcggaaaaa atatttcgat gaattaatat ataatatatt gaatagtaat
     5041 taatttttat atgaacagaa aacatttaaa atgtacgtta tggaaattac tttggacgct
     5101 cggtgtattg tcgctcattg ctgcatgggt ttcgtcacag tcggatccgg tgttggaatt
     5161 tgagtggagt ttttggtttt ggaacgcgct tgttttcgga gttctcgctg ttcccatcaa
     5221 gctagactgc cactcgtgtg acgtttgcgg tattggaaag taaagatttg aaatcaaaca
     5281 ttgctatcgt ggctgggatt ttagctattc gacaatctcg agtattgaat caagaacaat
     5341 tggaagttcg aaacagctat ctgcgatgcc gctaaaatgt cctttgccgt gttcgataaa
     5401 tatttttgaa ccaagcttgt tttcaaattc gtcgcgattg tcgagcggta cccacgggtc
     5461 attgtcagaa aaaacagcaa tcgtttttcc gatttgtttc ttaactttat ctaaatcaat
     5521 aggtgtggta agccagtgac gatcagtttc ccgaacagcc gtatcgtcct cgaggccagt
     5581 aagtcgcttc aaaaaaccgg caataaaaac ggctccgcca accttttttt tctcgggaag
     5641 tgattctaga tatcgaataa ttgtttggca ccccatacta tgtcccacaa agtaagtatt
     5701 tgtatctgcc gtgccgacag atttagcgag cgctggaacc cagttgtaga ttcttggatt
     5761 tgccgcgtcc ggcatctgcg ggataagcac ttcaaaacct agtttttcta attcgttttt
     5821 cagccatgga aaccaacctt cttcggggtg cccatcccaa ccgtgaacaa taatgactct
     5881 ttttttcgac ataaattgag ttattttaat cagcaatatt ttgccaaccg acgttcgcac
     5941 aatttcacga ccctgactcg cgggtcgcac tactgtgtcc acaccgctcc gtctcgcaca
     6001 tgtcttgaaa aatctaaagc agtttttttc tgtggtcaca gataattcac ttgccgccgc
     6061 aagcaaattt ccgcgacccc gcctcggccg tttcgtctgg tgacgaaaca tgcctgcggc
     6121 tcgcacacgt acgaaaaaac caagagcagt cgaggtttct tcgcggtggt caggaataat
     6181 tttttcgccg acgctcaaaa attttcacga ccctagctcg cggttcgcgc tactgtgctc
     6241 acaacgctcc gtctcgcaca cgtcttgaaa aatctaaagc agtttagatt tttaaagcgt
     6301 gtgcgccctg caggactcgg acctgcgacc tttccggtgt aaacggactg ctctaccaac
     6361 tgagctaagg gcgcgtgaga ataactatat acacacattt ttccaaaatc aaatttttga
     6421 atacccccac ccatttttgc actctgctgg taattgattc acataatacg ttgatcttgt
     6481 aagcacctca agcggtgtca ttttgttctt gaaagcataa tggactcttc tggtgttgta
     6541 gaacgtaaga tattcggaaa gtttttcgtt gaacagtgtt gtgttgtcga aaagaagatt
     6601 gatatggtaa tcaacaaact cctcttggat cgtcccattg aagctctcac agtgagcatt
     6661 catcttggga gtcttgggat aggtgtggta gtgggtaatg tggttttgtg agagtagttc
     6721 ggaaagacat ttcttgaatt cagagccgtt gtccgtcagt acattacgta tttcatacgg
     6781 aaacatccgc atcacaagaa agaagaaatg ggcaaaggtt tttgaagaat gtgacttggt
     6841 ggcaatggca aaaactgttc ttgtgaatat atcaatggct actagtacat acattcttct
     6901 gccatgtctc tgcttctcaa tggtatcaag tgccagtaca tgtccaggat acgttgggac
     6961 taaatcttta ggttttctca acactttctg gcgattggct tttttgatct tgccgaaatg
     7021 agagaccttt tgaggagagg ttcgaagacc ccctaagtca acgatcagtc tttcaatagt
     7081 ggaagaacct ggacgctttg caataccctc tacatcagca aaatcgagca ggagaggata
     7141 tatcttgtcg gcaccgagat tgggatactt gtttcgaagc cttttgattt cttcaattag
     7201 tttcgcatcc catactctct tacgcttgtt cttgggagtc tttttcttgg ggttgagcgc
     7261 ttccagcttt cctcctcctt ccttgagtaa ttgctgccat atgaagaggg tgcgtttctt
     7321 cactttgaat gcttcctctg ttgcttctgt tccgtgcttt tgccagaaag ccaggatccg
     7381 gcagcgttcc tttgcttcat tggtgatcat ataccggaag cgtatcactc gctgccatgt
     7441 tgctatgaac cctttggtat tcttgaattt attcccgatt ttcatcgtgc gcccattgtg
     7501 ttaatgagtg caatatgtat ctgaggttag tcacttttac atttgatttt atatttgata
     7561 cacgaaaatg gcggtaagat atagtgatgt tactatatta cgctatagca tgatataatc
     7621 acgtgtggga aggtatgaag acttcaagat gaaaaaaaga aaaataggaa aaaaccaacg
     7681 gaaaatttta ttgtttttgc tcggaggaat cgcgctcggg ctgtcggcaa gccctaggaa
     7741 atattttcaa gttatttctg ctctacgaga tgagtggaag gacatcgacc gacggcatct
     7801 taagaaagct
//
LOCUS       DATDSU010000034        10044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_194408, whole genome shotgun sequence.
ACCESSION   DATDSU010000034 DATDSU010000000
VERSION     DATDSU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194408"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VI981_01070"
     CDS             complement(<1..695)
                     /locus_tag="VI981_01070"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEY4478933.1"
                     /translation="MNGKPLILGVITARAGSKGIPGKNIKLLGGKPLIAYTIEAAKKS
                     KTITHLVVSTDGEEIAKVARECGADVPFVRPAELAADKVKHVPVMIHAVDFMEKKLGL
                     TFDATVILQPTAPFRLPEDIDETVKKLLNSKADSAVSLVEVEGANHPIKVKKLVDDRV
                     YSYCMEEEEGIRRQDLPRAYRRNGAVYVIRRDVLMKQKKLYGDYIMGHVVAEERSIDI
                     DIPRDWVMAELML"
     gene            complement(750..1586)
                     /locus_tag="VI981_01075"
     CDS             complement(750..1586)
                     /locus_tag="VI981_01075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4478934.1"
                     /translation="MAELVVAEMFQASGCFEPHVLELGPGVGISTEVLLEHGAKVIAA
                     EPNESMRYFFALNHMGDTRVRLVAGSAEEQNVEAGSVSCIVGCQMFHLLKDKLKAVLH
                     EAWAALHPAGFLTFDLGPSNWGSWSYKLHDFRSAIPRCFPDELMTELSHPLYQAVHNG
                     ALKFVQGSFPEFKRPNLWAEPAKAFTMDELLSAFAAAGFRLARITETAVPIVGPRIKE
                     FVRNSWTTWCRWAPLDALPIDEKLEIVQAGFDSLAKLGDRYNGVTAYHPTVIITAQKV
                     DP"
     gene            complement(1956..3170)
                     /gene="neuC"
                     /locus_tag="VI981_01080"
     CDS             complement(1956..3170)
                     /gene="neuC"
                     /locus_tag="VI981_01080"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEY4478935.1"
                     /translation="MKKTRRKKAVRKLKLKGGALKRKVCFVITSFIHYSRNLFVLEEL
                     KNHPGVELHILIGGTALLSKYSSKFAHILEILKRDGYRNIYELHFNVEGSDHIAKAKT
                     VGLGITEFSTYFNHIRPDVVVVRGDRFEVLAAAAAAASMNIPVAHIEGGDLSGTLDES
                     IRHSITKLSHIHFTTNDEARKRVIRMGEDPNYVFNFGSPDIEVVSRIVNGKTRADLSK
                     TGSGADFDFTKPYLMVMFHPVSTEVDHIREQTRLLASAIHELGVPTLWFWPNFDAGAE
                     ELSHELRTFRDEVPNIKIRFMRYLAPKDFISLLKNTSCLVGNSSAGIKESSLLGIPVV
                     NIGSRQQNRLRGKNVVDVDYHGEKIKDTITKQLKVGRYPKSSLYEGKDTSKRIAGILA
                     TVKLYVQKKFVE"
     gene            complement(3171..4289)
                     /locus_tag="VI981_01085"
     CDS             complement(3171..4289)
                     /locus_tag="VI981_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEY4478936.1"
                     /translation="MKAKTGKTIRIPGTKKSLNKDEVFVIAEIGKNFIDSKRDESVDV
                     YIENAKQLITAAKEAGVDAVKFQTHEVEDEQLDVKVVSPHFDGADRYSWVKKNTDATP
                     PRFWQEIKSHADKEGIIFFSTPMSRKAAEKLQALDMPLWKVGSGDVQDYRMLDFQIET
                     KKPIIISSGMVSLAELDRVIEHLKSKHASFAVLYCVSQYPCPREAFNLGTIEYLKEKY
                     PNLIIGFSDHSVGDDTVMLAAVKLGARIIEKHFSFSRKLWGSDHKVSMTPDEMKKMVG
                     KIRDGSYKNVDERLFYGEKHKELEGAENKFRPYFNKALVAGRDIKRGDVVTKEALYSM
                     RPLMHIKGERADRFYDIVGKCAKRNLKKFEPIKARDFE"
     gene            complement(4380..5183)
                     /locus_tag="VI981_01090"
     CDS             complement(4380..5183)
                     /locus_tag="VI981_01090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4478937.1"
                     /translation="MTRPCYDLYYKEAYRSDRNSIKGTSSETDLDRNYQNAKRFGKAL
                     ARKYKKYFRPGLALDVGSSTGGILAGLKEVLPTLEIKGIEPSEAEASFANKHGIPTVR
                     MLFEDFRDDLGGVQNILCVQSLNHLLDPAGFLRRSHEMLAPDGHIFLAVKNFRHQVRR
                     AGAFEAGVQIDHPYMFTPETLSRLVRRAGFHILDLDIDEGKTIEEIAAQKQNGFNIHH
                     IRLVARKGGKDESLPSKFEWLLTRLQFSRLSIKLHYLVQYSTRFSFLRK"
     gene            complement(5329..6288)
                     /locus_tag="VI981_01095"
     CDS             complement(5329..6288)
                     /locus_tag="VI981_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4478938.1"
                     /translation="MFNYAQAEYESVPCNFCMGVDVHTLSRTGQDGLPLHAVMCRNCG
                     LIFINPRMTREWYFKYYQGEYREKTIEHGTDGRKFNIEAMYKKALSHGERLGKTLREN
                     ITHPQLIVEVGSSAGGVLKGLQNVLGGQVLGVEPSEKEANFAIAHGVKTHVALFENVD
                     QRLLPSCSLVVCTQSLNHLLDPRAFLAWAHDRLETNGILALEVVNFRHQLKDAGKFKN
                     AVKIDHPYMFTPEVLCDFVRSAGFEILKCVNDEDLRATDLDKRIISIHTQLIARKREG
                     KSFSNIIHTPNLYRKNKCDLNPFRAYYGYLIKKRLPKLFSKNV"
     gene            complement(6360..7304)
                     /locus_tag="VI981_01100"
     CDS             complement(6360..7304)
                     /locus_tag="VI981_01100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4478939.1"
                     /translation="MRTSKQKKLTKQFFNAVADEWYDRTYNPSGDFVKFPSNKVRMEV
                     VLKEIVKLGLQGNVLDIGCGAGQLVIELLKKGYDASGIDIADEMIARAEENLKKSKLK
                     IQKRGIFKTADLAEFRSAKKYKIATALGLLEYLKSDAELFSVLDKVTEKGGYVLAECR
                     NKFFNLFSMNEYTLGVMNEIPELLRRFGEVQKYSAESDDQIPFIEAKVSKAIAAFLSK
                     ATKNISLLSTDVPNYSRYPQKMIRRQHTPQEIEASAKKFGFELLYVIYWHVHPYAPAY
                     EKKFPRIYNKMSYLMTPLGRTSLGAYLCSSFIGVFKKK"
     gene            complement(7301..8191)
                     /locus_tag="VI981_01105"
     CDS             complement(7301..8191)
                     /locus_tag="VI981_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY4478940.1"
                     /translation="MGNAIIVNYHYIRDESTNGIRACSIERFREQLVSLKKKYKIVSV
                     PDAYKAALENVNGNFCALTFDDGLVEHYKIAFNFLKQQNVTGTFFVIGSSLKEKTVPA
                     THKLHLLLSKKSSNELADLFHAFFGNRFLLSKTIRLNPKRRFDDVLTSNLKESLIALP
                     AEDRNSFIDSVFGQLYGDETQIFDELFMSAENVKEMHENGMTIGSHTYSHQALDGRDR
                     MDQALDIERGMEVVSDIVNAPVKALSYPHGRYDKNTLEILEEKKFSWAVILGGRAVKA
                     RDHKFCIPRYDTNDISQILK"
     gene            complement(8184..9551)
                     /locus_tag="VI981_01110"
     CDS             complement(8184..9551)
                     /locus_tag="VI981_01110"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEY4478941.1"
                     /translation="MKKVIFISIFEGVEAKNILRTPILDTLLQEPNIQIVLFTKSQER
                     VEYYKKTFNDPRFIYEVVKPPNIRGLDVIFSVLKFTLLRTRTTDLKRRMALTSSRNYA
                     LFFAASIINRLSARPFVRKLFRFLDLMLVRNTMYDQYFERYKPSLVFLAHLFDEPEIH
                     LLRATKKRKIKSIGFINSWDKVTARSILRLLPDKCVVFNAIVKEELMTHNEIKENDIF
                     IGGLPQYDSYLTTPPTDKREFFKKIGFGSDVERIIVYAPNGRYSASVDGGMIDVLLSF
                     MKEKQLPSDVGLLVRFQPNDFVDPTKFTKSDRLVYQMPGIRFSGERGVDWDMDTGDIK
                     LLINTLYHATLFVCYTSSLSVDAAVFDKPIINIKFELKPSADLRQSPTLYYEMDHYRK
                     AVATGGIKIVQSKDELLKWILLYLNNSSLDHQERRRLVSEQCGIVDGKAGERIGTFIL
                     NYLHG"
     gene            complement(9548..>10044)
                     /locus_tag="VI981_01115"
     CDS             complement(9548..>10044)
                     /locus_tag="VI981_01115"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEY4478942.1"
                     /translation="LVRFPFTDTVAYLDNKKNYGRIFFDRPEMNFELHKNNELSKEGE
                     KHLRLTLALCSMVVTGPSTICIDAAVFDKPIILVAFDGREKRKPLESIARYYMYDHWK
                     PIRASEGASFAGSEQELATLIERYIRNPALHSGGRKKIVETELEYTDRKSSERVADVL
                     KSML"
BASE COUNT         2712 a         2335 c         2044 g         2953 t
ORIGIN      
        1 tcgagcatga gttcggccat aacccaatct ctcggaatat caatatcgat tgaccgctcc
       61 tctgcgacaa catggcccat aatataatcg ccataaagct ttttttgttt cattaaaaca
      121 tcgcggcgaa tgacatatac agctccattt ctgcgatagg ccctaggaag atcctgtctg
      181 cgaattccct cttcttcttc catgcagtac gaatagacgc gatcatcgac gagcttttta
      241 actttaatcg gatgattcgc tccctccacc tcaacaagtg acacggcgct atctgccttc
      301 gagtttagga gttttttaac agtttcatca atgtcctccg gtagacgaaa cggcgccgtc
      361 ggctggagaa tcactgtggc gtcaaaagta agacctaatt ttttttccat aaaatcaaca
      421 gcatgaatca tgaccggcac atgctttact ttgtccgctg caagttctgc cgggcgtaca
      481 aaaggaacat cggcaccgca ttctctcgct actttggcaa tttcttctcc atcggtagaa
      541 acaacgaggt gcgtaatcgt tttgctcttt tttgcagctt caatggtata agcgatgagc
      601 ggtttaccgc caagtaattt tatgtttttt ccgggaatgc ctttggaacc ggcacgcgca
      661 gtgataacgc cgagaattag aggtttgcca ttcatgacct tgagaatatc ataagtacaa
      721 acaaaaaaaa gccgcctgca gtgcaagggc tatgggtcaa ctttttgtgc tgtgatgata
      781 accgtcgggt ggtaggcggt cacaccgtta tatcgatcgc caagtttcgc taacgaatcg
      841 aaccccgctt gaacgatctc gagcttctca tcaatgggaa gcgcgtcaag aggcgcccac
      901 cgacaccacg tcgtccagct gtttcgtacg aactccttga tacgaggacc aacaatggga
      961 acggctgtct ctgtgatcct cgcaagtcga aacccagccg ccgcgaatgc ggacagcaac
     1021 tcgtccatgg taaatgcctt tgccggttca gcccacaaat tgggcctctt gaattcagga
     1081 aaactacctt gaacaaactt cagcgcacca ttgtgcaccg cctgatagag cggatgcgac
     1141 agctcagtca tgagctcgtc cggaaaacaa cgaggaattg cggagcgaaa gtcatgaagc
     1201 ttgtatgacc aactccccca attcgacggg ccaaggtcga aggtcagaaa gcccgcaggg
     1261 tgaagcgcag cccaagcctc gtgaagcacg gccttgagtt tatccttgag aaggtggaac
     1321 atctgacacc cgacgataca actcaccgat ccagcttcca cgttttgttc ttctgcggaa
     1381 cccgcaacga gccgcacacg cgtatcaccc atgtgattga gagcgaaaaa gtagcgcatg
     1441 gattcgttcg gctcggcggc gataaccttg gcgccgtgtt caagcaacac ttccgtactg
     1501 atcccgactc cgggcccgag ctcgagcaca tgaggctcga aacatccgct tgcctgaaac
     1561 atttcagcaa caacgagttc cgccatttgc cgatagtagg gatcgcgact tgcgaaggct
     1621 tggtagggcg cgccgacatt accgacaaac agtttcgatg ctagtggact ttgttggtca
     1681 gacacaaatt gcctttcttc ttgatggttt agaacaaatt catccttctc gcctaagacg
     1741 agcgatactc tcttttaaca catatttttg ccaaacgcag gtgcacgtaa tggttcaata
     1801 gcttggcaac acctctcgag cagacagtgc tcactcatgc gctctcacac acttttaaac
     1861 tcaaatataa tatagaaacg atcgtttcta tattatattt taagtttata acatttaaaa
     1921 cggagacaga agtatatata agttacagtt gtcgcctatt ccacaaattt cttttgaacg
     1981 tagagtttta ccgtggcaag aatgcccgca atgcgcttcg acgtgtcttt tccttcgtag
     2041 agcgaagact ttggataccg ccccacttta agttgtttcg tgattgtatc ttttattttt
     2101 tcaccatgat aatcaacatc aacaacattc tttccccgca agcggttctg ctgccgtgat
     2161 ccaatattga cgacaggaat tccgagcaat gaagactctt tgattccggc tgatgaatta
     2221 ccaacaagac agctcgtatt ttttaaaagt gaaataaagt cttttggcgc aagatagcgc
     2281 atgaaccgaa tcttgatatt aggcacttcg tcacgaaacg tgcggagttc atgagaaagt
     2341 tcctcggctc cggcatcaaa attcggccag aaccagagtg tcggcactcc aagctcatgg
     2401 attgcacttg caagcagccg cgtctgttcg cggatgtgat cgacttcagt cgatactgga
     2461 tgaaacataa ccatgagata cggttttgtg aaatcaaaat cagcaccgct acctgtcttt
     2521 gataaatcgg cgcgtgtttt tccatttaca atgcggctca ccacctcaat atcagggcta
     2581 ccaaaattaa atacgtagtt cgggtcttcc cccatccgaa tgacccgctt gcgggcctcg
     2641 tcattggtcg taaagtgaat atgagagagt ttagtgattg agtggcgaat gctttcgtca
     2701 agtgtaccgg agagatctcc tccttcaata tgggctacgg gaatgttcat acttgcggcg
     2761 gcggcggcgg cggcgagcac ttcgaaacga tctcctcgga cgactacgac gtcgggtcga
     2821 atgtgattga aatatgtcga aaattcggtg ataccgaggc caacggtttt tgctttagcg
     2881 atgtggtcag acccttctac attgaaatgg agctcgtaga tgttgcgata tccatcccgt
     2941 tttaaaatct caagaatatg cgcgaatttt gaagaatact ttgaaagcag ggcggtgccg
     3001 ccgatcaaga tatgaagctc aaccccagga tggttcttca gctcttcaag aacaaacaaa
     3061 ttgcggctgt agtgaataaa gctcgtaata acaaaacaga ctttgcgttt taacgcgcca
     3121 ccctttagct ttagttttct tactgctttt tttcgtcgtg tttttttcat ttattcaaaa
     3181 tcccgcgcct taatcggctc aaactttttt aaattgcgtt tagcacattt cccgacaata
     3241 tcataaaatc gatcagcccg ctcgccctta atatgcataa gaggtctcat agaatacagt
     3301 gcctcttttg taaccacatc accccttttg atgtcacgtc ccgctacgag tgctttgtta
     3361 aagtaaggcc taaatttatt ttctgctcct tcaagttctt tgtgcttctc tccgtaaaac
     3421 agtctctcat caacattttt gtatgatcca tcacggattt taccaaccat ttttttcatc
     3481 tcatccggcg tcatcgagac tttgtggtcg gacccccaca acttgcgtga aaaggaaaaa
     3541 tgcttttcga taattcgcgc gccgagcttt accgcggcaa gcatcaccgt gtcgtcaccg
     3601 accgaatgat cagagaagcc gataatgaga ttaggatatt tttcttttaa atattctatg
     3661 gttcctaaat tgaaagcctc tctcggacat ggatactggc tgacacagta cagtacggca
     3721 aaagaggcgt gctttgattt aagatgttct atcacccgat ccaactctgc aagactaacc
     3781 atccccgacg atatgataat cggtttctta gtttcaattt ggaaatcaag cattcgatag
     3841 tcctgtacat cgcccgagcc cactttccag agcggcatgt cgagcgcctg aagtttctcc
     3901 gcggcttttc tcgacatcgg agtcgaaaag aaaattattc cctctttgtc tgcatgagat
     3961 tttatttctt gccagaaacg tggcggggtt gcgtctgtat tttttttaac ccaactatat
     4021 cgatcagcgc cgtcaaagtg cggcgaaaca acttttacat ccaactgctc atcttcgact
     4081 tcatgcgttt gaaatttaac cgcatctacc ccggcttctt tcgcggcggt gataagttgc
     4141 ttagcgtttt ctatataaac atcaaccgac tcatcacgct ttgagtcgat gaagtttttt
     4201 cctatttctg caataacaaa aacttcatcc ttattgaggc ttttttttgt gcccggaatt
     4261 ctaattgttt tgcctgtttt tgccttcatg agcaatagat ataatgttta tttaggccgt
     4321 atcgttacga agtggagaaa tatatcgtaa cacaatattt tatctttgta cagattacac
     4381 tactttcgta ggaaactaaa acgcgtcgag tactgtacga gatagtggag tttaatcgac
     4441 aatcggctga attggagacg agtcaaaagc cattcaaatt tactcggaag actctcatct
     4501 ttcccccctt ttcgagcgac gagtcgtatg tgatgaatgt taaatccatt ttgcttttgg
     4561 gctgcaattt cttcgatagt tttgccttca tcaatatcta gatcgagaat atgaaatcca
     4621 gcgcgccgca ccagtcggct aagcgtctcg ggcgtaaaca tatatggatg atcaatttgg
     4681 acccctgcct caaacgcgcc ggcgcgcctc acttggtgtc tgaaattttt cacggctaga
     4741 aaaatatgcc catcgggcgc gagcatttcg tgcgagcgtc tcaaaaatcc tgcggggtca
     4801 agaagatgat tgaggctctg aacgcacaag atattttgga caccacccaa atcatctcta
     4861 aagtcctcga aaagcatgcg gacggtggga ataccatgct tattggcgaa gcttgcttcg
     4921 gcttccgagg gctcgattcc ctttatctct agtgtcggta aaacttcttt caaccctgct
     4981 agaatgccgc ctgtacttga accgacatcg agcgcgaggc cgggcctaaa atatttttta
     5041 tattttcgag cgagcgcttt accgaaacgc ttcgcgtttt gataattccg atcgaggtca
     5101 gtttcagaac tcgttccctt gatactgttg cggtcagaac gatatgcttc tttataataa
     5161 agatcgtagc atggtcgggt cattctcgga gttatatata ttagactgca cttcgtgcac
     5221 atatgagtct tcacacgaag tccattcttc gatcggcgcg cgattagtcg catagtgtcc
     5281 gcgccgcaca gattgcaaac tacttcctca aaatcttgcg tattgaaatc aaacattttt
     5341 agagaacaat ttaggcaggc gtttttttat gagatatcca taatatgctc gaaacggatt
     5401 aaggtcgcac ttattttttc tatacaaatt cggcgtatga atgatattcg aaaatgattt
     5461 tccctctctt tttcgagcaa tgagttgcgt atgaatgctg ataatccgct tatctaagtc
     5521 agtagctcgc aaatcttcgt cgttcacaca ttttaaaatc tcgaatcctg cagatcggac
     5581 aaaatcacac aatacttccg gcgtaaacat atatggatga tctatcttaa ccgcattttt
     5641 aaatttgcca gcgtccttca attggtgcct gaaattcacg acctcaagcg caagaattcc
     5701 gtttgtttcc aaacgatcgt gtgcccacgc gagaaaagcc cgcggatcta ggagatggtt
     5761 gagcgattgg gtacagacga caagtgaaca cgaaggaagc agtctttggt caacgttttc
     5821 aaaaagagcg acgtgtgttt ttacgccgtg agcgattgca aaattagctt ctttctccga
     5881 tggttcaacg ccaagcactt gcccgccgag aacattttgt aatcccttaa gaacacctcc
     5941 ggcgctcgac ccgacttcaa caatcagttg gggatgcgta atattctcgc gaagcgtttt
     6001 tccgagtcgt tcgccgtgag agagcgcctt tttgtacata gcctcaatat taaatttgcg
     6061 gccgtccgtg ccatgctcga tcgttttctc acgatattct ccctgataat atttaaaata
     6121 ccactcccgt gtcattcgag gatttataaa aataagcccg caatttctgc acatgactgc
     6181 atggagaggt aatccatcct gacccgtgcg ggaaagcgtg tgcacatcca cgcccatgca
     6241 gaagttgcac ggtacggatt catattcggc ttgagcatag ttaaacattt gttaaataat
     6301 tttggcaggc gcctctttat gagatatctt gcgtaattac aaaagcattc aaatctctct
     6361 tatttttttt tgaatacacc gataaaagat gagcagaggt acgctccgag cgaagtacgt
     6421 ccaagcggcg tcataaggta agacattttg ttgtatattc tcggaaattt cttttcatac
     6481 gcaggcgcat acgggtgaac atgccagtat ataacataga ggagttcgaa cccaaatttt
     6541 ttagctgatg cttctatttc ttgcggagta tgttgccgac gaatcatctt ttgaggatat
     6601 cgggaatagt ttggaacatc cgttgaaagc aaacttatat ttttcgtcgc ttttgaaaga
     6661 aatgcagcaa tagctttaga tacctttgcc tcaatgaacg gtatttgatc atctgattca
     6721 gcgctgtatt tctgaacctc tccaaaccgc ctaagcaatt caggtatttc attcattact
     6781 cccaatgtgt attcattcat agaaaatagg ttaaaaaatt tattacggca ttcagcaagc
     6841 acatatccac ccttttctgt cactttgtcg agcacggaaa acagttctgc atcacttttc
     6901 aagtattcga gcaatcccaa ggccgtggca attttatatt ttttcgcgga tctaaattcc
     6961 gcaagatctg cggttttaaa aatacctcgt ttttgaattt tgagcttcga ttttttgaga
     7021 ttttcttctg ccctcgcaat catttcgtcg gcaatatcaa ttccgctggc atcgtatcct
     7081 ttttttagta gctcaatgac aagctggccc gccccacatc caatatccaa tacattgccc
     7141 tgtaagccga gctttactat ctctttgagc actacttcca tgcggacttt attacttgga
     7201 aatttaacaa agtctccgga tggattatat gtcctgtcat accactcgtc ggcgactgca
     7261 ttaaaaaatt gcttcgttaa ttttttttgc ttgcttgttc tcattttaat atttgcgaaa
     7321 tatcattcgt atcataccgc ggaatacaaa atttatgatc acgcgccttc acagcgcgcc
     7381 ctcccaagat aacggcccat gaaaatttct tttcttcaag tatttctaga gtgtttttat
     7441 cataacggcc gtgagggtag gagagtgctt tgacaggggc gttgacaata tcactcacca
     7501 cctccatgcc acgttcaata tccaatgctt gatccatgcg gtccctgcca tctaacgctt
     7561 ggtgagaata tgtgtgagaa cctatggtca taccattttc gtgcatttct tttacatttt
     7621 cggctgacat aaacagttca tcgaatattt gtgtctcatc gccgtataat tgaccaaaaa
     7681 cactatcaat aaaactattt ctatcttcgg cgggcagagc aattaaggac tctttgaggt
     7741 ttgaagttaa aacatcatcg aatctgcgtt tcggattcaa acgaattgtt ttacttagta
     7801 gaaaacggtt tccgaaaaaa gcgtgaaata aatcggcaag ttcattcgaa cttttttttg
     7861 aaagaagtaa atgaagttta tgcgtcgccg gaaccgtttt ctcttttaga gaagaaccta
     7921 taacaaaaaa agtgccagtg acgttctgtt gcttcagaaa attgaaggca atcttataat
     7981 gctcaacgag accgtcatca aatgtaaggg cacaaaaatt tccattcaca ttttcaagcg
     8041 cggctttata tgcatcaggt acagatacga ttttatattt cttttttaat gatacaagct
     8101 gctctctaaa ccgctcaatc gagcaagctc gaatgccatt tgtactttca tctcggatat
     8161 aatgataatt tactataatc gcgttaccca tgtaagtagt tcaaaatgaa agtaccaatg
     8221 cgctcccctg ctttgccatc aacaattcca cattgctcgc tgactaatcg tcttctttct
     8281 tgatgatcta atgacgaatt gtttaaatac aataaaatcc acttcaagag ttcgtcctta
     8341 ctctgaacga tcttaatccc tcctgtcgca acagctttcc tgtagtggtc catttcatag
     8401 tagagcgtag gagactggcg caaatccgcg ctaggtttta actcaaattt gatgttaata
     8461 attggtttat cgaacacggc ggcgtcaaca ctaaggcttg aagtataaca aacaaataaa
     8521 gtcgcgtgat aaagtgtgtt tatgagtaat ttaatatccc ccgtgtccat gtcccaatcc
     8581 actccgcgtt cgccagaaaa ccgaataccg ggcatttgat atacaagtct gtccgacttg
     8641 gtaaattttg tcgggtcaac aaaatcattc ggctggaatc ttactagaag tccaacgtcg
     8701 gaaggaagct gtttttcctt cataaacgat aacagaacgt ctatcatccc gccatctacg
     8761 gatgccgagt accgcccatt tggcgcgtat actattattc gctcgacatc cgaaccgaag
     8821 ccgatctttt taaaaaattc ccttttgtcg gttggcggag tcgttagata ggaatcatat
     8881 tgcggcaagc ctcctatgaa aatatcattt tccttaatct cgttgtgggt catgagttct
     8941 tcttttacaa tagcgttgaa aacaacacac ttatctggta aaagccttaa tatcgaacgc
     9001 gccgttactt tatcccatga atttataaat ccgattgact ttatctttct ttttttagta
     9061 gctcgtaata agtgaatttc tggttcatca aagagatgcg ccaagaaaac aagtgaaggt
     9121 ttataacgtt caaaatattg atcatacata gtatttcgca ccaacataag atcaagaaac
     9181 ctaaagagtt ttctcacaaa cggtcgagca gagagccgat ttatgataga agccgcaaaa
     9241 aataaagcgt aatttcgact tgacgttaga gccattcgtc gtttgagatc agtggttctg
     9301 gtacgcagaa gcgtaaattt taacacacta aaaattacat ctagaccgcg tatgttggga
     9361 ggtttaacaa cttcatatat aaatcgtgga tcattaaatg tttttttgta atactcaact
     9421 cgctcttggc tttttgtaaa aagtacaatc tgaatattcg gctcttgaag aagcgtgtca
     9481 agtatgggtg tacgcaaaat attttttgct tcgactccct caaaaattga aatgaatatt
     9541 acttttttca tagcatcgat tttaaaacat cggcaacacg ctcgctcgat tttcggtctg
     9601 tgtattcaag ttccgtctcc acaatttttt tccttcctcc gctgtgcagt gccggattcc
     9661 taatgtaacg ttcgataagt gtcgcaagtt cctgctccga accggcaaac gacgccccct
     9721 ccgaagcgcg tatcggcttc caatgatcat acatgtagta gcgcgcaatg ctttcaagcg
     9781 gcttgcgttt ctcccttccg tcaaaagcca cgagaataat aggcttatcg aaaacggccg
     9841 catcaatgca aatcgtcgaa ggacccgtga caaccatcga gcacaatgcc aacgtgagac
     9901 gtaaatgttt ttcaccctcc tttgaaagtt cgttgttttt atgaagttca aaattcattt
     9961 ccgggcgatc aaaaaatatc cgtccgtagt tttttttgtt atccagatac gcgacggtgt
    10021 ctgtgaatgg aaaacggaca agaa
//
LOCUS       DATDSU010000035         5395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1202990, whole genome shotgun sequence.
ACCESSION   DATDSU010000035 DATDSU010000000
VERSION     DATDSU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202990"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VI981_01120"
     CDS             <1..306
                     /locus_tag="VI981_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEY4478943.1"
                     /translation="RHRPHLRFREPAARERQQARFKAIQNVATNSDMGPKELEQLAEL
                     SSAGEKAIMNAARAYKLSARGYHRTIKLARTIADLAGNPTIDEPHMMEALQYRVREM"
     gene            479..2344
                     /locus_tag="VI981_01125"
     CDS             479..2344
                     /locus_tag="VI981_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY4478944.1"
                     /translation="MKRAAHKSKTNRSEVNAYRFIYDELTTKKGWKKDQVYEQQECLE
                     IKGIKQSLGTLKPENVVAIDTTRYYIVEAKNERKKMEQAIKEATEYSDLINSKSDLRA
                     IFATGVAGNDAEGFVAKSAYLKGGKWETITENDIELTSLLSKNQVELILNTKTAKIKD
                     VEISSEEFFKVAGEINTILHDNSINKDYRARFISAILLALSDDTEINLNERNASVLIS
                     LINAKVAAILNKHNKAAFSRFIRIDDPSSEDNHIKVRAAIIRTIQELLGLNIRSAMRS
                     GRDILGQFYEVFLKYGNGAKEIGIVLTPRHITSFAAEVLDIRSNDLVLDPACGTGGFL
                     VAAFDEARKKIKSKVEFDNFRENGLYGIEEQDPVIALAIVNMIFRGDGKNNMIEGNCF
                     NKWLTLKTSGGVMTAEYRGEDFQGRIPPITKVMMNPPFAQKSSKSKEYQFINQALMQM
                     QNEGILFSVLPLSVLIEKATADWRKDLLKHNTLLSVVTFPEDLFYPTGTHTLGIFIKK
                     GIPHKKDSPALWIRALNDGFVKKKGKRVKSERAKDDISEIKELLKSFIKDQSIKVKNK
                     PQLQKKALIDFADESFELIPELYVDDKELSQEEIEFSMESLMRETASFLIRTKEK"
     gene            2341..3282
                     /locus_tag="VI981_01130"
     CDS             2341..3282
                     /locus_tag="VI981_01130"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEY4478945.1"
                     /translation="MKIQELFTIENARSKGFEQHKKGRVPFVTNGFHSNGVVGFVEVL
                     KGERIFKENAICVSAFCEATVQYPPFLPRGNGGSGLIVLIPKTQMEDAELYYYAAQIN
                     RQAWRFSFGRMVIQERIAHLSIKSSEKKPLRFSVKDLLPKEKARRDMKIFEFKIFPLT
                     ELCSIERKYAPYINQLNLSVQKTPYITTTETDNGISIWCNEKANFPKGTLTVSLDGQC
                     GKAFYQFDDFLAGEKTAVLSLKDKNNPDLLFYIGTIIEKMSWRYNYGVKLSMARLQEF
                     QIPIPVNSDGKIDLSSVQTMVYNTYGSEMFEKYSVTA"
     gene            complement(3299..4390)
                     /locus_tag="VI981_01135"
     CDS             complement(3299..4390)
                     /locus_tag="VI981_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEY4478946.1"
                     /translation="MSFRVKGMSLRADVVVDLQYGDCGKGKVTHYLAHTKKYTHVLRY
                     NGGCNAGHTIFHNGKRFVTHQVPAGVFFGIKSIIGSGCVVSPEQFFAELKMLEEGGVD
                     TKGKIFIARNTHVITKANKAEDKQVGGAIGTTGQGNGPAYRDKYGRTGVRAEKTPELE
                     PYLIDLYEEWFTPSSTLRDEPSGSDSNESTVEGLPDINILAEGAQGFGLDIDWGDYPF
                     VTSSHCTTAGALLNGLPPNAVNDVWGVAKIYETYVGSKKFQPEGEIFDTIGDIGEEFG
                     ATTGRRRQVNWMNMQTLERAIRMNGVTHVVFNKIDVLREAGEWAVIDQDKVVPFNDEK
                     TMQDFVDERLKTFAIKPENIFFSESKERI"
     gene            complement(4640..>5395)
                     /locus_tag="VI981_01140"
     CDS             complement(4640..>5395)
                     /locus_tag="VI981_01140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4478947.1"
                     /translation="PAGTIADIDKPIDGTISLYVVRAGDTVSSIATMYDVSPNTILAA
                     NNLPKGAKLKEGQKLTILPVTGIKYTVKKGDTLASVAKRYGGNMDEIISRNDIEDGTL
                     TVGDEIIIPNVDVPTVAKPKATARGTVASTSGTQIGSFLAPLARYVRTQGIHGYNAVD
                     LAAPSGTPVRAAAAGKVVVAKSSGWNGGYGSYVVVQHAGGSQTLYAHMSRVAASDGES
                     VDKGDVIGYVGQTGIATGPHLHFEIRNGVRNPF"
BASE COUNT         1529 a         1185 c         1280 g         1401 t
ORIGIN      
        1 cgccaccgtc cgcatttaag attcagggaa ccagcggcgc gggagcgaca gcaggcgcgt
       61 ttcaaagcca tacaaaatgt cgcgaccaac tccgatatgg gccccaaaga gctcgaacaa
      121 ctcgccgaac tctcttcggc gggggagaaa gcgatcatga acgcggcccg agcgtacaaa
      181 ctctctgctc gcggctacca ccgcacaata aaactcgcgc gcacgatcgc cgatctcgca
      241 ggaaacccga ccatagacga gccgcacatg atggaagcgc tgcagtatcg ggtgcgagag
      301 atgtagttta cccaaggggt tccattggaa aaactcgtaa attgggcctc gttttagggg
      361 aaatttacgt gtttttggca ttttttagtg aatcgtcacg aagtaccggg tgtcggaaat
      421 gcaccggaag ctgaaaagca tggcttttca aattttctaa acttgctaca cttggcatat
      481 gaagcgagcc gcgcacaagt caaaaacgaa tcgctcggaa gtcaatgctt ataggttcat
      541 ctatgacgaa ttgacaacaa aaaaagggtg gaagaaggat caggtatacg aacagcaaga
      601 gtgtctggaa attaaaggca taaaacaaag tctcggcact ttaaaaccag aaaatgtagt
      661 agcgattgat acaacaagat attacattgt cgaagcaaaa aatgagcgca aaaaaatgga
      721 gcaggcaata aaagaagcga ctgaatattc tgaccttatt aactcaaaga gtgatcttcg
      781 agcaattttt gctactggag tagccggcaa tgacgcggag ggctttgtgg caaaaagtgc
      841 ctatcttaag gggggaaagt gggaaacgat tactgaaaac gatatcgaat taacaagttt
      901 attgtcaaag aatcaggttg agttaattct taacaccaaa actgcaaaaa taaaggacgt
      961 cgagatttcg agcgaagaat ttttcaaggt tgcgggagag attaatacaa ttttacatga
     1021 taattcaata aataaagatt acagagcaag attcatctcg gccattttac tagctctatc
     1081 cgatgatact gaaataaatt tgaacgagcg aaacgcctca gttcttattt cacttatcaa
     1141 cgcaaaagtt gctgcgattc tcaataaaca caataaagct gctttctcac gtttcattag
     1201 gattgatgat ccatcgagcg aagataatca cataaaagtt cgcgccgcga taattagaac
     1261 aatccaagag ttgctgggac ttaatatccg ttccgcaatg aggtctgggc gcgatatttt
     1321 gggtcaattt tacgaggtgt tcctaaaata tggcaacgga gcgaaggaga tcggtatagt
     1381 gttgactcct cgacatatta ctagttttgc agcagaggtt ttagacatac gaagcaatga
     1441 tttggttctg gatccggcct gcggtaccgg agggttttta gttgctgcgt ttgacgaggc
     1501 gagaaagaaa ataaaatcaa aggtggaatt tgataatttc cgtgaaaacg gactttacgg
     1561 gatagaggaa caagaccctg taattgcact tgcaatcgtt aacatgattt ttagagggga
     1621 cggcaagaac aatatgattg agggtaactg tttcaataag tggttaactc ttaaaacatc
     1681 aggaggggtg atgactgcag aatacagggg tgaagatttc caaggcagaa ttccacccat
     1741 aactaaagtc atgatgaatc ctccgtttgc tcaaaaatcg agcaagagca aagaatatca
     1801 atttataaat caagcgttga tgcagatgca aaatgagggc attttgtttt ctgtactgcc
     1861 tctttcagta cttatagaga aagctacggc cgattggcga aaagatttat tgaaacacaa
     1921 tacattactt tcggtcgtta cattcccaga agatctcttt tacccaactg gcacacacac
     1981 cctgggaatc tttatcaaaa aaggcatccc gcataaaaaa gattctcctg ctttatggat
     2041 tcgtgcatta aatgacggtt ttgttaaaaa gaaagggaaa agagtaaaaa gtgagagagc
     2101 taaagatgat atttcggaaa ttaaggaatt gcttaaaagt tttattaaag atcaatctat
     2161 aaaagtcaaa aataagccgc aactccaaaa aaaagccctc attgattttg cggatgagag
     2221 ttttgagtta ataccagaat tgtatgtgga cgacaaagag ctctcccagg aagaaataga
     2281 gttttcgatg gaaagtttga tgagagaaac cgcgagtttt ctcattcgta caaaagaaaa
     2341 atgaagattc aagagctatt tacgattgaa aatgcgcgct caaaaggatt tgagcagcac
     2401 aaaaaagggc gagttccttt cgtgacaaac ggattccaca gcaacggcgt agttggcttt
     2461 gttgaagtgt taaaggggga acgaatcttc aaggagaatg ctatttgtgt ctctgcattt
     2521 tgtgaggcta cagttcaata tccacccttt cttccacgag gaaacggggg tagtggtttg
     2581 attgttttaa tccccaaaac tcaaatggaa gacgctgagc tttattacta cgctgctcaa
     2641 ataaatagac aagcctggcg tttttctttt gggcgaatgg ttattcaaga aaggattgct
     2701 cacttatcca tcaaatcatc agaaaaaaaa cctctgcgtt tttctgttaa agatttgctc
     2761 ccaaaagaaa aggctaggcg agatatgaaa atttttgagt ttaagatttt tccgttgact
     2821 gaactatgtt cgattgaaag aaaatatgcg ccctacatca atcagttaaa tctatccgtg
     2881 cagaaaacac cctatataac aaccacagaa acggacaacg gtatttccat ttggtgcaat
     2941 gaaaaagcca actttccgaa ggggacactg actgtttcgt tagacgggca atgtggtaag
     3001 gccttttatc aatttgatga tttcttagcc ggagaaaaaa ctgctgtgct tagtctaaaa
     3061 gataagaata accctgactt gcttttctat ataggaacaa taatcgaaaa gatgtcttgg
     3121 cgctataact atggcgtaaa gttatcaatg gcacgattac aagaatttca gattccaata
     3181 ccagtaaatt cggatggcaa gattgatctt agctctgtcc aaacaatggt ctataacact
     3241 tacggttccg agatgtttga aaaatactcc gttactgcct aggatttctc tgtcagaatt
     3301 aaatccgttc cttactctcg gagaaaaaga tgttttcggg tttgatggcg aatgttttga
     3361 ggcgctcgtc cacgaaatcc tgcatagttt tttcgtcgtt gaatggaacg actttgtctt
     3421 ggtcgatcac ggcccattct cctgcttcgc gcagtacatc tattttgttg aagacgacgt
     3481 gcgtgacgcc gttcatgcgt atggcgcgtt cgagcgtttg catattcatc cagttgacct
     3541 ggcggcggcg gccggtcgtt gcgccaaatt cttcaccaat gtcaccgatt gtgtcgaaaa
     3601 tctcgccttc gggctgaaac tttttgcttc cgacatacgt ttcgtaaatt tttgcgacac
     3661 cccacacgtc gttcacggca tttggcggca atccattcaa aagtgcgccg gccgttgtgc
     3721 aatgggaaga ggttacaaaa gggtaatcgc cccagtcgat atcgagtccg aaaccctgcg
     3781 cgccctctgc gagtatattg atgtcgggca acccctcgac agtgctctca ttcgagtctg
     3841 agcctgaggg ctcatccctc agagtcgaag acggggtaaa ccattcctca tagagatcga
     3901 tgaggtaggg ctcgagctcg ggcgttttct cagcacgcac acccgtacgg ccatatttgt
     3961 cgcgatatgc aggtccgttg ccttggcccg tggtaccaat cgcgccaccc acttgcttat
     4021 cttccgcttt gttggccttg gtgatcacgt gcgtgtttct agcgatgaaa attttgccct
     4081 tggtgtcgac gcccccttcc tcgagcatct tgagctccgc aaaaaattgt tcgggagaaa
     4141 cgacacagcc cgatccgata atggatttaa taccaaaaaa cacaccggcg gggacctggt
     4201 gtgtgacaaa cctttttccg ttatgaaaaa tggtgtggcc tgcgttgcag ccaccattgt
     4261 agcggagcac atgggtgtat ttcttggtat gcgcgaggta gtgggtaacc ttgccctttc
     4321 cgcaatctcc atactggaga tcgacaacga catcggcgcg tagcgacata cccttcacac
     4381 gaaatgacat cgcttttgat cgatgtcatt tataactata tggtaaacgt accaaagagg
     4441 gtgattgtct tctctgtcgg aaaaacctac agcaacagag attatgccat ttcgtgtgcg
     4501 gggcatattc tttcggctgc cgtattgtag cacgtttcaa agcgactagc tgctagcttc
     4561 tagcatttag tttgaatcca aagccagaaa ctggtccctg tctactggcg gccaggcagg
     4621 cttgagttgc tagaagcttt taaaacggat ttctcacccc attacgtatc tcaaagtgta
     4681 ggtgcgggcc cgtagcgata cctgtctgcc ccacataccc gatgacatca cccttgtcca
     4741 cgctctctcc atcggatgcg gctactcgag acatgtgcgc ataaagggtc tggctgccac
     4801 ccgcgtgttg gaccactacg tagctgccat atccgccatt ccatccactg cttttggcga
     4861 ccaccacctt cccggctgcc gcagcgcgaa ccggtgtccc tgaaggagcc gcaaggtcga
     4921 cggcattgta gccatgaatg ccttgcgtcc gcacatagcg ggcaagcggc gcgaggaatg
     4981 aaccaatctg ggtcccagag gtactcgcca ctgtaccacg cgcggtggct ttcggcttcg
     5041 ccaccgtcgg cacgtcgaca ttggggatga tgatctcatc tcctacggtc agggtgccgt
     5101 cttcgatgtc gttgcggctg ataatctcat ccatattgcc gccgtagcgc ttggccaccg
     5161 aggcaagcgt atctcctttt ttcaccgtgt atttaatgcc tgttactggc aaaatcgtca
     5221 gtttttgacc ttctttgagt ttggctccct tgggcaaatt gttggcggcg agaatggtgt
     5281 tgggagaaac gtcgtacata gtggcaatcg acgaaacggt atcgcccgct cgcacgacgt
     5341 atagagaaat ggtgccatcg atcggcttat cgatatcggc gatcgtccca gcggg
//
LOCUS       DATDSU010000036         4535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_868065, whole genome shotgun sequence.
ACCESSION   DATDSU010000036 DATDSU010000000
VERSION     DATDSU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868065"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(616..2796)
                     /gene="topA"
                     /locus_tag="VI981_01145"
     CDS             complement(616..2796)
                     /gene="topA"
                     /locus_tag="VI981_01145"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY4478948.1"
                     /translation="MKLLIVESPAKAKTISKYVNELGGKHEYVVKASVGHIRDLPKSN
                     KKAIDIEAGFVPHYEISSGKEKVVSELKSLAKKATEVLLATDPDREGEAIAWHIVEAT
                     GIKNPKRIVFHEITKEAVQEAIKHPRAIDENLRRAQEARRVLDRLVGYDLSGLIWKKV
                     RYGLSAGRVQSPALRILMEREREIRAFKSHAFWVIEAALETEAKKKLTLVCEKEPTVK
                     KEVDEILERAKKEQWKVKDVSETEAKRAPRPPFITSTLQQSASSRLGFAPSRAMSVAQ
                     RLYEAGYITYMRTDSTNLSSQALGQIHALISKKYGVQYLSPRTFARKSKNAQEAHEAI
                     RPTNAAVESAGSNDEQKKLYRLIWQRAVASQMADARIMRTKITAEVGSHATPDFFANG
                     SRTLFNGWLAADVGASGEEIELPKVKVGETLMLDEIRTEEKATQPPQRYSEAGLIKEL
                     EKRGIGRPSTYASIIRTIEDRGYVSKENKALKPTDTGDVVSSFLEKHFTEYISDSFTA
                     EMEDKLDNIAQGQAEYKKTLSDFYKPFLKDVKSKDKLEKITTLGDAPKEFPCPVCGAA
                     MVIKLGKSGKFMSCSKFPKCLGARTLEGKELEGPRETGEMCPECKIGKLIERDGRYGR
                     FIACSNYPKCKFIKKDAELEKQASTGVKCNVCNEGYMTEKRGRFGIFYSCSNYPKCKN
                     AMKARPTGNLCPICGKLMMLGTKTIPERCSDKMCPNHNPHKLIK"
     gene            complement(3312..4166)
                     /gene="dprA"
                     /locus_tag="VI981_01150"
     CDS             complement(3312..4166)
                     /gene="dprA"
                     /locus_tag="VI981_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY4478949.1"
                     /translation="MSMRKLDFKNFPLLLKEIPDPPKHLYMEGKLPSDDNKFLAVVGA
                     RKYSEYGKEATEMLISGLRGFSIVIVSGLALGIDAIAHRAALGAGIKTIAIPGSGLSR
                     KVLYPSVHHSLAENILASGGTLLSEFEPEFRATPWSFPQRNRIMAGLAHAVLVIEAEQ
                     KSGTLITSRLATEYNRDVFTVPGSIFSKNSEGPHMLLRLGATPVTNPKELLQAIGFKQ
                     DDVPAVVDFANCSPEEIKVLELIETPLAREDLVRALHISTSEANALLSIMEIKGLIRE
                     SMGEIHRA"
     gene            4207..>4535
                     /locus_tag="VI981_01155"
     CDS             4207..>4535
                     /locus_tag="VI981_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478950.1"
                     /translation="MAKKVLLVCSSTMVDLLRAACQRAHAEVLIAGTLAEAAHMLMQS
                     PDLVVWELMLPDGDARPLIQATRNAKPLLEMVGVSGDTDERLMQFGAGCNRTSGPFHA
                     ITLVAEIL"
BASE COUNT         1029 a         1057 c         1075 g         1374 t
ORIGIN      
        1 atgagacggg attattattt tgcagccgtt ggtacgggca agataataaa aaaacccagc
       61 attaactagc tgggagacaa atgtctctta cgacttgcgc caacttaagc gtcggccaaa
      121 gtcgaaacgt atcgcgggcg aagagcgtct cggcctcgtt gtcgttgtct cgttcagttt
      181 cccgagaatc ccgagaatga ttgcgagcat ggttttttgt ggttacctgt agtttgtgca
      241 cgactcgacc gaaaatcata tcatgactga caagatggtg acgcacgata catcaaaaac
      301 atatatctgt caatctgaaa atctagaggt cagcgataaa cacccggggt tttagagcta
      361 caccccgcct tctcttttat accatttccg ccggccgtcg cccgacacac cgaatagtgg
      421 cgtgatatta agtagaaatt taatcatcag tagtgcgaag ctagagagta tttcgctctt
      481 ataccccgca atgtcaaggg cggcccttga catcggagcg tctgaagtat tttgccctgg
      541 ctgtggcggg caggcgcgca aataatgcaa tagaagtttt atgcccattc tcgatctggt
      601 gtgccgcgga tagaattatt ttatgagctt atgcggatta tggttgggac acattttgtc
      661 actacatcgt tccggtatgg tttttgtccc aagcatcatc aactttccgc agatcgggca
      721 gaggtttcct gttggtcttg ctttcatggc atttttgcat ttaggataat ttgagcaact
      781 gtaaaagatt ccaaagcggc cgcgtttttc tgtcatgtaa ccctcgttgc atacgttgca
      841 tttaacgcct gttgaggcct gcttttcgag ttccgcatct tttttgatga acttgcattt
      901 tggataatta ctgcaggcga tgaagcgtcc gtagcgccca tcgcgctcga ttaattttcc
      961 gatcttgcat tctggacaca tttcgcctgt ttctctaggc ccctcgagtt ctttgccctc
     1021 gagcgtgcgt gccccgagac attttgggaa tttggagcag gacataaatt ttccgctttt
     1081 gcccagtttg atcaccattg cggctccgca gacggggcag ggaaattctt tcggagcgtc
     1141 gccaagtgtc gtaatttttt ccaatttatc ttttgacttc acgtccttta ggaatggttt
     1201 ataaaagtcg cttaatgttt ttttgtattc cgcctgcccc tgtgcaatgt tgtcgagctt
     1261 atcttccatt tcagccgtaa atgagtcgct aatgtattcg gtgaaatgtt tctccagaaa
     1321 tgaactgacg acgtctcccg tatctgttgg cttcagggct ttattttctt ttgaaacgta
     1381 accgcgatcc tcaattgtac gaataataga tgcatatgtc gaaggtctgc cgatgcctcg
     1441 tttttctagc tctttgataa gtcctgcttc ggagtagcgt tgcggcggct gggtggcttt
     1501 ttcttcagtt ctaatttcgt caagcatgag cgtctcgccg actttaactt ttggcaattc
     1561 tatttcttcg ccgcttgctc cgacatctgc cgcgagccat ccgttgaaaa gtgttcgtga
     1621 tccgttggca aaaaagtcgg gcgttgcatg cgaccccact tctgctgtta tttttgttcg
     1681 catgattcgc gcgtcggcca tttgcgaagc aacggctctc tgccaaatga gccgatataa
     1741 ttttttctgt tcatcatttg atcctgccga ctcgactgcc gcattggtag ggcgaatggc
     1801 ctcgtgagct tcttgagcgt tcttgctctt gcgcgcgaat gtgcgcggac ttaagtattg
     1861 cacgccatat tttttcgaga taagcgcgtg aatttgtccg agcgcttgtg aacttaagtt
     1921 tgtgctgtcc gtacgcatgt aggtgatgta tcccgcctcg taaagtcgct gggccacaga
     1981 catagctctc gatggagcaa agccaagccg ggagctcgct gattgctgaa gggtggaggt
     2041 gataaatggc ggtcgaggag ctcgttttgc ttcagtttca gaaacatctt taaccttcca
     2101 ctgttctttt ttggcccttt ctaatatttc gtcgacctct tttttgacag tgggttcttt
     2161 ctcgcacaca agcgtgagct tcttttttgc ttcggtttcg agagcggctt cgattaccca
     2221 aaacgcatgg gacttgaaag ccctgatttc acgttcacgt tccattaaaa tgcgaagagc
     2281 aggggattga acgcggccgg cggaaaggcc ataacgaact tttttccaga tgagccccga
     2341 taggtcgtat ccgacaagtc tatctagcac gcggcgtgct tcttgggcac gtcttaggtt
     2401 ttcatcaatt gctctcgggt gttttatcgc ttcttggacc gcttctttag tgatttcatg
     2461 aaagacgatg cgtttgggat ttttaatccc cgtggcctct acaatgtgcc atgcgatcgc
     2521 ttcgccttca cggtcggggt cggtcgcgag caatacctcg gtcgcttttt ttgcaagaga
     2581 tttcaattcg gaaaccactt tttcttttcc gcttgagatt tcataatgcg gcacaaaacc
     2641 ggcttcaata tctatggcct ttttattgct tttgggaaga tctcggatgt gaccaacaga
     2701 tgcctttaca acatactcat gtttacctcc aagttcgttt acgtattttg aaattgtttt
     2761 cgctttggcc ggcgactcta ctataagtag cttcatcccg ttagagatag gaaatgccca
     2821 actttgcagt gaacgtttgc tatcgttaaa taccaattgc taaatcgacc ttttttaata
     2881 tttatatatt caggcatttc cgatatttac ttccgataga gaagttacct gagttcattt
     2941 tatgattgaa aagttataaa aaacataaca taacaatctc taacgggact catgcgatca
     3001 agtattacac gatttaaaaa ttaaaagcaa atttttaaaa aaaagacgcg ccgggacgat
     3061 ggagatcgtt cccggcgcgg gacagtcaag ttacgtgtac gtgccgcgtg atagcgcgag
     3121 agatacgccc ggaaccccgc tctctgactt aacaagacag cggttgttat cctgtccaga
     3181 tttttcccaa aaaaccgatt ccaattatta tgacaggaag tcacatgtaa ttggtttgct
     3241 ctgacgatga gcaagcttgc ggcttttgct ccgtcctcgc cgccaattat acaagtggcg
     3301 gaaaaatttt tctacgcacg atggatttcc cccatacttt ccctaatcag tccctttatt
     3361 tccattatag aaagcagggc gttggcttca cttgtggaaa tgtgtagcgc gcgaactaaa
     3421 tcttcccgtg cgagcggtgt ttctatgagt tcgagcactt ttatttcttc cggcgagcag
     3481 ttcgcaaaat cgactactgc gggcacatca tcttgtttaa atccaatggc ttgtagcaat
     3541 tctttcggat ttgttactgg tgtggccccg agcctcaaaa gcatatgtgg gccctctgaa
     3601 tttttagaga aaatggaccc cggtaccgtg aatacgtctc tgttgtattc ggttgcgagt
     3661 ctcgacgtga taagtgtgcc ggatttctgt tctgcctcga tgacgagtac tgcatgcgcg
     3721 agcccggcca tgatgcgatt tcgctgtgga aagctccatg gagtggctct gaattccggt
     3781 tcgaactcgg agagaagcgt gccgccggat gctaatatgt tttcggcaag actgtgatgg
     3841 acgcttggat acaaaacttt tcgagacagt cctgaacctg gaattgcgat agtttttata
     3901 ccggccccaa gcgccgctct gtgagctatg gcatcaatac cgagtgctaa tcctgaaacg
     3961 atgacgatcg aaaaacctcg caggccggag ataagcattt ccgttgcttc ttttccatac
     4021 tcgctgtatt tccgcgctcc cacgaccgct aggaatttat tatcgtcact cggcagtttg
     4081 ccctccatat aaaggtgttt tggcggatcc gggatttctt tgagcagtaa tggaaaattt
     4141 ttgaaatcca attttcgcat tgacattcac atattgtaac ttttgttgta atgtaatgga
     4201 agatttatgg cgaaaaaagt ccttctcgtt tgtagcagta ctatggtgga ccttctccga
     4261 gccgcgtgcc agagggctca cgcggaagtg ctcattgccg gcacgcttgc cgaggcagca
     4321 catatgctta tgcagtcgcc ggatcttgtc gtgtgggagc tcatgcttcc tgacggcgat
     4381 gcacggccgt tgattcaggc aacaagaaat gccaaaccat tacttgaaat ggttggagtg
     4441 agcggtgata ctgatgaacg cctgatgcaa tttggtgcag gttgcaaccg cacttcaggg
     4501 ccttttcatg ccatcacgct tgtcgccgaa atact
//
LOCUS       DATDSU010000037         3654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_537295, whole genome shotgun sequence.
ACCESSION   DATDSU010000037 DATDSU010000000
VERSION     DATDSU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537295"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..1099)
                     /locus_tag="VI981_01160"
     CDS             complement(365..1099)
                     /locus_tag="VI981_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4478951.1"
                     /translation="MNAKESLISLGLSVQEADVYIALLKQGGASASVLARDIGMKRTT
                     AYAILKNLTEKGFSSVYFRSGKRVYHAQKPHKIASLVEQKLQSFNEAIPFFESMDRAQ
                     TQKLGLRFIESKNELEKFYDEILIDYKNKEYRIIGSTPAWESIDPRYFVKFRENRAKA
                     NIHTKLLLTAESKKDSPTDKDLLRQVRFLPKERAFKSTLDIFDDKVLIVSPDLASLAV
                     IVAVPAMVDIFKSIFEMLWESVSDTD"
     gene            1226..1933
                     /locus_tag="VI981_01165"
     CDS             1226..1933
                     /locus_tag="VI981_01165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4478952.1"
                     /translation="MKEREKQPAIPTNLNYHYYGKNKYDRDIVSAIPFHREIHESILK
                     ELQRRFQPDQVIRVLELGVGTGLTTSLIRAAFPNAEFELVDFSSTMLSHAKKRLGTKK
                     IVYRLGDFSKIRFHSPCDVVVAVIGIHHLSHSGKRNLFQKIALLLKPGGIFLFGDLMT
                     CADEKDAAERSARHYHHLVEHAGSRSMLRDWTHHHMFLNNLATIEDQEKWLQDTGCRV
                     KRLFYQWNTALLLATKQ"
     gene            1938..3278
                     /locus_tag="VI981_01170"
     CDS             1938..3278
                     /locus_tag="VI981_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY4478953.1"
                     /translation="MEKIMPIVNGTNTFVDLVTEKHVRVVKLNNAATTPPFVQTVSAL
                     HTFFDSYGALHRGAGPHANKTVEITEKALGTIREFIGVSPEQTLLFSSNTSAAINFFA
                     RLMTLSEQDVILTSCIEHTSNNLPWRYNTRAKIVEVAAFSDGSLDMEDFTEKAELHGA
                     NLKWIAITGASNLTGYVLDMKRLSRIAHTHGARLFVDAAQLAPHRPINMTEQGIDALA
                     FSAHKIYSPFGLGVLVLPKDMLDRTPVDPGGGSIDMISEKNIVWAPSAERHQTGTWNV
                     TGIIALAESCRVMMEVGWKTIVDHERELVEYTAKSLAEIPDLTLHVPLEKFLSEDRIG
                     TFPFTLRGYHHALLAAILDHEYGIETRAGTICNHRLVRRWFTVDDDAQKEIEERIKNG
                     DRLASYGAVRASVGIFNTKGDIDRLVDALKSIQKNGAQLQYRPMPREETYSPEL"
     gene            complement(3345..>3654)
                     /locus_tag="VI981_01175"
     CDS             complement(3345..>3654)
                     /locus_tag="VI981_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY4478954.1"
                     /translation="LQDALIHSDQGFHYTNPLYIEQIKGLNMIQSMSRRGNCIDNAPI
                     ESFFGHLKDDVDYKDCKTFDELHSLIATYMQYYNNERQQWDLKKMTPVGYRNHLLTPA
                     "
BASE COUNT         1040 a          781 c          818 g         1015 t
ORIGIN      
        1 cgggcgcaaa gccatcgata aagcacaaaa gtgagcagtt aaactcactc tgtcgcgatc
       61 tgcggggtgt aatcaacggt atccgaactg gggtatagaa ataccatctc ctaaacatcc
      121 acagtcgaat tctgtgggct tttaggaaac aactttctcc agagataaaa gctaagagca
      181 gcgccggcga tattaataag aattcctaaa tacaagtgtg catctatggg agtgttatta
      241 attaaagcat tttccaccgt atccccatac gggagtattt ctgaaaagcg ggagctactc
      301 gggaatttac ggagcaggtt ggtgtaccta aacaagcaag tgactttaat gcaggagagc
      361 cacattaatc cgtgtcacta acgctctccc atagcatctc aaagatcgac ttgaaaatat
      421 caaccattgc cggaacagca acaatcacgg cgagcgatgc tagatctgga cttacgatga
      481 gcaccttatc gtcaaaaata tctaaagtac ttttgaaagc acgctctttt ggtaggaaac
      541 gaacctgacg aagaagatct ttatcggtcg ggctgtcttt ttttgattct gccgtgagaa
      601 gcagctttgt atgaatattt gcttttgcgc gattctctct aaattttacg aaatagcgag
      661 gatcgatact ctcccaggcc ggggtactgc caataattcg atattcttta tttttatagt
      721 caatgaggat ttcatcataa aatttttcaa gttcattttt gctttcaatg aaacggagac
      781 cgagcttttg cgtttgagct cgatccattg attcaaagaa tggaatcgct tcattaaagc
      841 tctgcagttt ctgttcaacc agcgaggcaa ttttgtgcgg tttctgtgcg tggtaaaccc
      901 gcttaccact tcgaaagtaa acggaagaaa accccttttc agtgagattt ttcaaaatcg
      961 cgtatgcagt cgtacgtttc ataccgatat cacgggcaag cacgcttgct gaagccccgc
     1021 cctgtttgag cagggctata tatacatcag cttcttgaac cgatagtccg agggatataa
     1081 gtgattcttt agcattcata ttgtcgtcaa aagtcttcga cactatttag tataactaaa
     1141 tttatagctt atattagcta attagtcaat gtagtatgac taaaattatt gactataacg
     1201 tcattttagg ctaatattat tcgctatgaa ggagcgagaa aaacaaccag ccatcccaac
     1261 aaacttgaat taccattact acggtaaaaa caagtacgat agggatattg tttccgcgat
     1321 acctttccat cgagaaatac acgaatcaat tttgaaggaa cttcaacgtc gttttcaacc
     1381 agatcaggta attcgtgttt tagaactagg agttggtact gggctcacta catctcttat
     1441 tcgtgcagca tttccaaatg ctgaatttga gttagtcgat ttttcttcga ctatgttgtc
     1501 acacgccaag aagcgtttgg gaacaaagaa gatcgtttat cgtctcggtg atttttctaa
     1561 aattcgtttt catagtccgt gtgatgttgt agttgccgtt attggcattc accatctatc
     1621 acactccgga aaacgtaatt tatttcaaaa gattgctctt cttctcaaac caggaggaat
     1681 attcctcttt ggcgatctca tgacttgcgc agatgaaaaa gacgccgcag aaagaagcgc
     1741 acgtcattat catcacttag tagagcatgc aggaagtcgc tctatgctcc gtgactggac
     1801 acatcatcac atgtttctaa ataacctcgc cacgattgaa gatcaggaaa aatggttgca
     1861 agatacggga tgtcgagtca agcgactttt ttaccagtgg aatacagcac tgcttctcgc
     1921 tactaaacaa tgatcttatg gaaaaaatca tgccaatcgt taacggaaca aatacatttg
     1981 tcgatctagt gacggaaaaa cacgtgcgag ttgtaaagtt aaataacgcc gctacaactc
     2041 ctccgtttgt gcaaacggta agcgctcttc atacgttttt cgattcgtat ggcgcactcc
     2101 atcgaggagc cggtcctcat gctaataaaa ccgttgaaat taccgaaaaa gctctcggaa
     2161 cgattcgtga gttcattggc gtatcaccag aacaaactct cctcttttct tccaatacga
     2221 gtgcggcaat taacttcttt gcacgactca tgacgctcag tgaacaggac gttattctaa
     2281 cttcatgtat tgaacacacc tcgaataact tgccgtggcg ctataacacg agggcaaaga
     2341 ttgttgaggt ggccgcgttc agtgatggct cgctcgacat ggaggatttt acagaaaaag
     2401 ctgaattgca tggcgcaaac ctcaaatgga ttgctattac cggagcctca aatttaacgg
     2461 ggtatgttct ggatatgaag cgcctttcta ggattgcaca cacgcacggt gcacgactct
     2521 ttgttgatgc cgcgcagctt gcaccacatc ggccaatcaa catgacggag caaggaattg
     2581 atgcattggc cttctcggcg cataaaatct attcaccgtt tggactgggt gtgcttgtct
     2641 tgccgaaaga catgcttgat cgaactccag tcgatcccgg aggcggatca atcgacatga
     2701 tttctgagaa aaatattgtc tgggcaccta gtgccgaacg tcatcaaacg gggacatgga
     2761 acgtaacggg tatcattgcg ctcgcggaaa gttgtcgtgt catgatggaa gtcggatgga
     2821 aaacgatcgt tgatcacgag cgagaacttg tagaatacac agcaaaaagt cttgccgaga
     2881 ttcctgatct gacgctccac gtgccacttg aaaaattctt atcagaggat cgtattggta
     2941 cttttccgtt tactctaagg gggtatcacc atgctttgct ggcagcgatt ctcgatcacg
     3001 aatacggaat cgaaacacga gcaggaacaa tctgtaatca ccgtcttgta cgtcgttggt
     3061 ttactgttga tgatgacgct caaaaagaaa ttgaagagcg cataaagaac ggcgatcggc
     3121 tggcttccta cggagcagtt cgcgcgagcg tcggtatctt taatacaaaa ggagatatcg
     3181 atcgcttggt tgatgcactg aaatctatcc aaaaaaacgg agctcagttg caatacaggc
     3241 caatgcctcg ggaagaaacg tatagccctg aattatagag tgtaggccca taacaaaacc
     3301 gcccgtgtga tctgtacccc gtaaagtaga cacggggaga aaaactacgc aggtgttaaa
     3361 agatgatttc tgtatcccac aggagtcatc ttttttaagt cccattgctg ccgttcattg
     3421 ttgtagtatt gcatataggt tgcgatgagt gaatgaagct cgtcgaaggt tttgcaatcc
     3481 ttgtagtcca cgtcgtcttt cagatgtccg aagaacgatt cgattggtgc gttgtcgatg
     3541 cagttgccgc gtctggacat cgattgaatc atgttcaatc ctttgatctg ctcgatatag
     3601 agcgggttgg tatagtggaa accttggtcg gaatggatca gggcatcttg gagt
//
LOCUS       DATDSU010000038        13196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_537809, whole genome shotgun sequence.
ACCESSION   DATDSU010000038 DATDSU010000000
VERSION     DATDSU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537809"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VI981_01180"
     CDS             complement(<1..868)
                     /locus_tag="VI981_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase catalytic subunit"
                     /protein_id="HEY4478955.1"
                     /translation="MRDNNSHIDKIFGRANEAPRLLLVINAVMALLYLYALVFWFSKG
                     ELYLYWILIVGQVFHTWQVLSFIYTIWDTGYTASGNRTHTPPADVFITVCGEPAEIVE
                     ETIRAALAMNYPYFTVYVLNDGFVAGKENWRQIEALAKSLGVVCITRRKPGGAKAGNI
                     NHALNHTSNPFVVLFDADHIPQTNFLREVMYYFSDEKVGFVQTPQYYRNHKLNYVTAG
                     AWEQQHLFYGPILKGKNRMNAVSMCGTNMAIRREALLEVGGMCDTNIAEDFVTGLFIH
                     EHGWKSVYVPKLL"
     gene            complement(872..2086)
                     /locus_tag="VI981_01185"
     CDS             complement(872..2086)
                     /locus_tag="VI981_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEY4478956.1"
                     /translation="MMLTKYRLIGMVLIVVAIVLAAAVIYRNSLRRQIPLVFSHKIIL
                     DSTWANYKNTYIERGSSRTLDKQRQNITTSEGQSYTMMRAVWLDDKSTFDSSWQWTKA
                     NLQRQDKLFSWLYGKLADGTFGVEQKNGASNSASDADVDIALSLIFAYERWREAPYLE
                     EALPILRSIWDNEVLLIGGKPYLLANNLEKNSNKEDVIVNPSYFAPYAYKVFFQYDRG
                     RQWLLLAGSSYDLLERAMNASLGGRQTKKLPPDWLVINRKTGELGAHENVELSTNFGY
                     DALRVPWRITLDWQWFADPRAKALLEQMQFLADEWNEHGLLSLYHHDGSIAEQREFPA
                     LYGGTIGFFSTVYPEIALEIYRNKLVILFDPDRQSWKKELSYYDDNWAWFGMAMYHKV
                     LPNLSAPRAGSQ"
     gene            2275..3399
                     /locus_tag="VI981_01190"
     CDS             2275..3399
                     /locus_tag="VI981_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HEY4478957.1"
                     /translation="MKKSITLSVFFPAFNEEENIHEAITRAISALQNIPAIDDFEVII
                     VNDGSTDTTGEVAERLARESNKIRVIHHEVNRGYGAALASGFRAARFEYIFFTDADLQ
                     FDLEEISKLLEHIPKYRVVLGYRAPRIDGFVRLFNAKGWNVLNRILFGLKVRDIDCAF
                     KLFDRKLIEKLPVNSRGAMISAEILIRLTRSGIRFKEVPITHLPRVAGSATGANLSVI
                     VRAFREMLSLYNGDLGTVTQKQVVKFIIVGVVNTIVDFVVYFFLSRTFFAFSAHRVAT
                     KTVSFLVGSLSSFFFNRRWTFGKTDAITATEVVRFYTTVALSVIINSSSMYVFHHMLG
                     FYDLISVVCATAITFAWNFFISKFWVWRDVKETDPLRLKT"
     gene            complement(3396..4268)
                     /locus_tag="VI981_01195"
     CDS             complement(3396..4268)
                     /locus_tag="VI981_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4478958.1"
                     /translation="MNIGIIFALLAVIFWTFGDFFIQRSTRKVGDVETLFWIGFVGSI
                     GIFWFIRDEVSFLVDVEKVLFLSGVGLLTLVAALLLFEGLKRGKLAIVEPIFGLELPF
                     TVFFSIVVTREFIGLSGYVYILIIFIGLLLTAVSHLHHLKFHRTIFEKGVPYAALGAI
                     LMGLMNYLTGIGARALSPLATIWFIHTFLAIVCFCIVVKRGHVGTLAKNIVTERATIF
                     PLAILDNAAWISYAYAMVFLPISIATALSESYIATAVLVGIFINKEKLKRHQIVGVCL
                     VVIGLFFLSKYASS"
     gene            complement(4406..4900)
                     /locus_tag="VI981_01200"
     CDS             complement(4406..4900)
                     /locus_tag="VI981_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY4478959.1"
                     /translation="MKRFHIFLGVILFLVVLAFFWFIIKKNDQEISQNQVPLEKTSPK
                     KSAEFMAGETTIRLEVAETDRDREQGLSGRANLGADTGMIFVFDEPDQYGFWMKDMKF
                     PIDILWLDRDKKIVTILESVSPNSYPKVFQPTEEAMYVIELEASKSRALGLTEGVTLM
                     LKGI"
     gene            complement(5393..5463)
                     /locus_tag="VI981_01205"
     tRNA            complement(5393..5463)
                     /locus_tag="VI981_01205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5429..5431),aa:Pro,seq:cgg)
     gene            5654..6778
                     /locus_tag="VI981_01210"
     CDS             5654..6778
                     /locus_tag="VI981_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478960.1"
                     /translation="MPPGENLFRQEGPEVPAPKPEHQLESREATIARVGEILKEGIDA
                     LVIHGTTIRRDGKETFVLRPDLDAMGGLYLMDIAGINYSMATSVPKGEKIPGKVHIDT
                     GGYGDTTIVQEDNEEESVFFDHHAEVKGEQTSATKLVYEALVENKLLTPEPWMEQFVE
                     FVTECDNLSYKVTPNHFKSTWAHSMYGQNRSLSAEQVANLFKSGHNPKSAFTQHQRQT
                     IQVLENRRRVMNLEEACAKRQKEVDRSVKGMDVSMKKMMANHLKIETPELGKIMINEI
                     HREGKKTIDEIPLGGRAANAMGYDTYIMTDGSGFFISSQTHDLEPIFEKIQKELPEAK
                     LVRGTMILYKSETHPVATRRLLEILNLFEDGAEHDPAVTS"
     gene            complement(6896..7333)
                     /locus_tag="VI981_01215"
     CDS             complement(6896..7333)
                     /locus_tag="VI981_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4478961.1"
                     /translation="MRKGFDYIGISIVYFCHDGKGNFVMAKRNNNTRDEHGRWDIGGG
                     GIEFGDSVEDTLRREIKEEYCTDILDFEFLGFRDVHREHDGRKTHWVALDYRVLVDRL
                     RVKIGEPEKFSEIGWFTLGTVPENSHSQWPTFLKRYRSKLGKS"
     gene            complement(7340..7999)
                     /locus_tag="VI981_01220"
     CDS             complement(7340..7999)
                     /locus_tag="VI981_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY4478962.1"
                     /translation="MNEENENEIDRLAKILWDYNHLNQTLKKADAVLVLGSYNPLVAE
                     RGIDLYFEGYAPYIIFSGNVGRFTRGVFKKPEAEVFADIARERGVPESAIIIENTSTN
                     TGENISFTKLLIEKMGLEIGSFIVVQKPYMERRAYAAFKKLWPEKEVTVTSPQCSYEK
                     YVEMTPGFKERILNSMVADTQKIKLYAEKGFQISQEMPDRVWRASERLVQLGFSKHLV
                     K"
     gene            8176..8583
                     /locus_tag="VI981_01225"
     CDS             8176..8583
                     /locus_tag="VI981_01225"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY4478963.1"
                     /translation="MNTSSVHVRQFFKFCIVGAVGIAAYYAILFVLTEGARVWYIASA
                     CIAFCFNQVVTFFFNKRWSFKCNDKTATRAQMRLYFVMGAVTLVGNAALLALLVEVCK
                     MHYLRAQFILIVVFSGISYFLTQKIFAKYIPPL"
     gene            complement(8961..9764)
                     /locus_tag="VI981_01230"
     CDS             complement(8961..9764)
                     /locus_tag="VI981_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478964.1"
                     /translation="MRNLNKGSLPTIVAVVVLLLIVGLGIVAYRGGKSEKKSDENAVA
                     GAEKRIEFDLLEQNNSGQMGKVLISEEDGKAKITITMSGKKTAEPQPAHVHIGMCDKI
                     GEIASELTNVVGGTSVTSYSLNFDGLVSHVLSFLPLSVNVHKSEAEPDVYVACGELPG
                     ETASEGINVNSSVSTLETVNLRYTQEGFIPKEITVQKGQKVRFINETKSPFSVASDPH
                     PVHTNFPAFDQSKSVFSGQTIFEFNFNEAGDWAYHNHLDASHKGVVIVR"
     gene            complement(9910..10887)
                     /locus_tag="VI981_01235"
     CDS             complement(9910..10887)
                     /locus_tag="VI981_01235"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEY4478965.1"
                     /translation="MNNEELLQELSTKISTGEIRREEVLNRLGFASATQSKVKAENLK
                     TSPTFSVTKMLYVLGAAIVVTGIVIFVSQIWADIGSFGRILVTLGLGFVITAAGSMLL
                     KSKPEDNIGTVFHFIGGMLIPGGAVVTLSELNLDSGDWTVAITFGAILAFYLLLNSIH
                     KNAILAFFAIANGTSFVYLVIEAIVDGPFYRHEDLYAYLTMVIGVSYLLLGHAFRDGR
                     NKKLIGFLYLFGISGFLGAAFSQVFNSVPWQMLYFLIVIGGLVLSVYMRSRSILVMST
                     LFLIAHVSYITSKYFADSLGWPISLVILGFLFIGLGYVSVNINKKYIAN"
     gene            complement(10910..11500)
                     /locus_tag="VI981_01240"
     CDS             complement(10910..11500)
                     /locus_tag="VI981_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478966.1"
                     /translation="MEFLKKNFAILLAFILPIMLIAVVALSTYIPSLFLSTDYNFVYT
                     ACTEGNNYYYPYRCDNYLQRRYVVVDNKLVVNPVDMTQDLDKNGVPDFNEKYTDRIFL
                     HDTKKNESREITLAEAQTLTLNNLVTSPDGVTVSGDYDRNGGDFFFIFGGGSSSYGYY
                     LTKGKSKTKINLINDTDQYYYRNNFGFVGWVLPGRN"
     gene            complement(11628..12218)
                     /locus_tag="VI981_01245"
     CDS             complement(11628..12218)
                     /locus_tag="VI981_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478967.1"
                     /translation="MSKKLLIIVFLIGVAVIGVLWSFGFGIHTVYEKSDSTEGIYTDD
                     ENTSSVFFDYDDGKLKSIKKTFLGILYNTPCFAFGCISKQYVFQLLPLGSREVSFKNK
                     CVLHYENLAETNKLIKDRGVKYEIPDRTGKGFVYTVECEPYWTEADENGEYYNNGFIE
                     RFDPVDDIASTYPQSLGILLKRNVSFPTEQNRIVPL"
     gene            complement(12426..>13196)
                     /locus_tag="VI981_01250"
     CDS             complement(12426..>13196)
                     /locus_tag="VI981_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478968.1"
                     /translation="VISVAIAALIIFLSKFIPYGFYIASFPVSFSGLDYPLALLGLNY
                     STWAIQIVAFLIYALLGIYIGLLFNRKYNWKQKAIITFIYLILFVPLSSYSHWLKRSD
                     KELAWQNTITAVYNPSKNNEFTSWFIPDYSVCDKLSVFTSVQERQSCLNGILLTNWQA
                     RSLDLFVQSDDGIKCLNDDYGNRNKWICVQTVAIEKQDVAMCKIHPFGPATDSCIAEI
                     AIAKKDASLCPQPPSQARIGGYDQSLYDSCLRRILEQN"
BASE COUNT         3849 a         3023 c         2657 g         3667 t
ORIGIN      
        1 taagcagttt cggcacatac acagacttcc agccgtgctc atgaataaag agtccagtga
       61 cgaaatcttc ggcaatattt gtgtcacaca tgccgccgac ctcaagaagg gcttctcttc
      121 gaatcgccat attcgttccg cacattgaaa cggcgttcat gcgattctta cctttcaaaa
      181 taggtccgta gaaaagatgc tgttgctccc acgctccagc ggtcacataa ttcaatttgt
      241 ggttgcgata atattgcggg gtttggacga agccgacttt ttcatcagag aaataataca
      301 taacttctcg gagaaaattt gtttgaggaa tatgatcggc gtcgaaaaga acaacaaacg
      361 gattcgacgt gtggttaagc gcatggttta tgttccctgc tttggcacct cccggtttcc
      421 gtctggtgat gcagacgacc ccgagcgatt ttgcaagcgc ttctatctgc ctccaatttt
      481 cttttccggc gacgaaaccg tcattcaaaa catacactgt aaaatagggg taattcatgg
      541 cgagcgccgc tcgtatggtt tcctcgacaa tttccgcggg ctctccgcat actgtaataa
      601 atacatctgc gggcggagta tgtgtcctat taccgctcgc cgtgtaccct gtatcccaga
      661 ttgtataaat gaaactaagc acctgccacg tatgaaaaac ctggccgacg attaggatcc
      721 aataaaggta gagctctccc ttggaaaacc aaaacaccaa ggcgtagagg tagaggagcg
      781 ccattacagc attgataaca agcagaagac ggggggcttc gtttgccctc ccaaaaattt
      841 tatctatatg tgaattatta tctcgcatag attactgtga tccggctctc ggagccgata
      901 ggttcgggag caccttgtgg tacatggcca ttccaaacca tgcccaatta tcgtcgtaat
      961 agcttagctc cttcttccat gattgacggt ctgggtcaaa aagtattact aatttgtttc
     1021 gatagatttc gagcgcgatt tccgggtaca ctgtggaaaa aaagcctatc gtgcctccgt
     1081 acaacgccgg aaattcacgc tgttccgcga tcgagccgtc gtggtgatag agagaaagca
     1141 gcccatgttc gttccattcg tcagccagaa attgcatttg ctcaaggagc gcttttgccc
     1201 ggggatcagc aaaccactgc caatcgagtg ttattctcca tgggactcgg agtgcgtcgt
     1261 agccaaaatt agtcgatagc tctacatttt cgtgcgctcc gagttcgcct gttttacgat
     1321 tgatcacaag ccaatcagga ggcaattttt tagtttgcct tccaccaagt gacgcattca
     1381 tcgcccgctc gagtaagtca taacttgacc ctgcaagcag gagccattgt ctgcctctgt
     1441 cgtattggaa aaaaacttta taggcatacg gcgcaaaata agacgggttt acaatcacgt
     1501 cctccttatt cgaatttttt tcaagattgt tggctaagag gtagggcttt cctccaatca
     1561 atagaacttc gttgtcccaa attgatctca aaatcggcag tgcctcttcg agatacggcg
     1621 cctctctcca gcgttcgtag gcaaaaatga ggctaagggc aatatcgaca tccgcatctg
     1681 atgcgctgtt tgacgccccg tttttttgct cgacgccgaa agttccatca gcgagttttc
     1741 cgtacaacca tgaaaaaagc ttgtcctgtc tttgtaaatt tgcttttgtc cattgccaag
     1801 aactatcaaa tgtgctctta tcgtcaagcc acaccgctct catcatggta tagctctgtc
     1861 cttctgatgt tgtaatattt tgacgttgtt tgtcaagcgt ccgagagctt ccgcgttcga
     1921 tgtacgtatt tttgtaattt gcccacgtcg agtcaagaat aattttatgt gaaaatacga
     1981 gcggaatttg ccggcgtaga ctatttctgt agataacagc cgccgccaaa acaatggcga
     2041 cgacaatcaa aaccattcct attaatctgt atttcgtgag catcatgatt ggccgttttg
     2101 gagcaagggt gtaaatggga taatactcaa aattacacga gacacaaggc cgtctctttt
     2161 ttgaaacatg gtcaattgtc tagacgcaag ggataccgtc gtaatatgag ccgtggactg
     2221 acgtctcaat acttgaaata agttgtgtag tattgtatgt attacccata ccccatgaaa
     2281 aaatccataa cactttccgt attctttcct gcattcaacg aagaagaaaa catccacgag
     2341 gctataaccc gtgctatttc ggcacttcaa aatatccctg ctattgacga ttttgaagtc
     2401 attatagtca atgacggcag tacggatact acaggcgagg tggccgaacg gctcgcgaga
     2461 gaatcaaaca aaatccgagt tattcatcat gaggtcaatc gcggttatgg agctgcgctt
     2521 gcgtcagggt ttcgcgccgc gcgatttgag tatatttttt tcacagatgc tgatctgcaa
     2581 tttgatcttg aggaaatttc aaaactgctt gaacatatcc ccaaataccg agtcgtgctt
     2641 ggataccgcg cgcctcgcat cgacggattc gtgcgccttt tcaacgccaa gggatggaat
     2701 gttctaaata gaattttgtt cggtcttaaa gtaagagaca tagattgcgc cttcaaactt
     2761 ttcgaccgga agcttataga aaaactgccg gttaattctc gcggcgcaat gatttcggcg
     2821 gagattctta ttcgtctcac cagatcgggc attcgcttca aggaagtgcc tatcactcac
     2881 ctgccccgtg tcgcaggctc cgctacggga gccaatttga gcgtcattgt ccgcgctttc
     2941 agagaaatgc tgtctctcta caacggagac cttggtacag ttacgcagaa acaagtcgtt
     3001 aaatttatta tcgtcggagt tgtaaacacg atcgttgact tcgtcgtcta tttcttcctc
     3061 tcgcgaacgt tttttgcatt tagcgcccac cgcgtggcca ccaagaccgt ttcatttctc
     3121 gtcggttcgc tctcgagctt ctttttcaat cgccgctgga cgttcggaaa aacagacgct
     3181 attactgcaa ccgaagtcgt acgtttttat acgactgttg cgcttagtgt catcataaac
     3241 agttcctcta tgtatgtgtt ccatcacatg ctcggattct acgatctcat cagcgtcgta
     3301 tgcgcaacag ccattacctt tgcgtggaat ttctttattt caaaattctg ggtatggcgc
     3361 gatgtcaaag aaaccgatcc gcttcgtctc aaaacttaac tcgaagcgta ttttgagaga
     3421 aagaacaatc ctatcactac gaggcatacg ccaactattt ggtgacgttt taatttctcc
     3481 ttgttaatga aaatccccac aagaactgct gtcgcaatat agctctctga aagagctgtg
     3541 gcgatcgaga taggcaaaaa taccattgcg tatgcatagg aaatccatgc cgcattgtcc
     3601 aaaatggcca acggaaaaat tgtggctcgt tccgtgacga tatttttggc gagagtgccg
     3661 acatgcccgc gtttcactac aatgcagaaa caaacaatag ccaaaaaagt atggataaac
     3721 catatggttg cgagcggtga gagtgcccga gctccgatgc cggtcagata attcataagc
     3781 cccattaaaa ttgctccgag tgcggcgtat ggaacacctt tttcaaatat cgtccggtga
     3841 aatttcaagt gatgcaaatg cgacacggca gtcagcaaga gtccgataaa aataatgagg
     3901 atatatacgt agccgctaag gccgataaac tcacgagtaa caacaatgct gaaaaaaacc
     3961 gtaaaaggaa gctctaagcc gaagatcggt tccacgatgg caagtttccc gcgcttcagg
     4021 ccttcaaaca acaaaagtgc cgccacgagc gtgagtaaac cgacgcccga gagaaaaaga
     4081 actttttcaa catcgacaag aaatgatact tcgtctctaa tgaaccaaaa aattccaata
     4141 cttcccacaa agcctatcca aaaaagtgtt tcaacgtcgc cgactttcct tgtacttctt
     4201 tgaataaaaa aatcgccaaa tgtccagaat ataactgcga gaagtgcaaa aataattcct
     4261 atattcatcc cgttagagat ctgcccgcca tcgcctaacg catagatggg aagcagtgca
     4321 attagttggt aatatcatct ttattttgtt gggctgaaag aacagtaata aaacatgact
     4381 atgtgatctt taacgggatc catactcaaa ttccttttaa cataagggtt actccttccg
     4441 tcagtcctaa ggcacgagat tttgacgcct caagttcaat tacgtacatc gcttcttctg
     4501 tcggttgaaa aacttttgga tatgaatttg gagaaacact ctcaagtatc gttacgattt
     4561 tcttatcgcg gtcaagccac aggatatcta tcggaaattt catgtccttc atccagaagc
     4621 catattgatc cggttcatca aaaacaaaaa tcattccggt atcagcgccc agattggcgc
     4681 gtccgctcaa accttgctct ctgtctcggt cagtctctgc tacttcaagt cttatagtcg
     4741 tctctcctgc cataaactcc gcgctctttt ttggacttgt tttttcaagg ggcacttgat
     4801 tttgggaaat ttcctgatca ttctttttta tgatgaacca aaaaaaagcc aagacaacaa
     4861 gaaataaaat gacgcctaga aatatatgaa atcgcttcat atggattgtt tttcagtata
     4921 catgagaaca agtggataag tcattgaact aaaaaaagac atgtgctaga gtaggcactg
     4981 acagcacaat gaaagcaaga tcgcctccgt aaccggtagc gttcccaggt tcaccctcgg
     5041 aaaacagcgg atttctatcc gcagtgtcga cacccgcgtt ctgccgtgtg aaaaagagac
     5101 tcaaagtggt tcgcccaccg gagtcggccc tcgtagccac attcacgcca tcagaattaa
     5161 aggatcggca cagtcttaac caacgtccgc gtcacttcgt cttccgtaag acaggtcacg
     5221 cggacgtccc tttttcagcg ctttgtgcgc ttttttaatt tgtcgggatg ccgagattcg
     5281 gctaccacgt agccaggtgc ttttccatct cgcagactcg ataaggaata gcctttcgaa
     5341 tccggccgca cgcaaagcag tttgcgtgct atcccttcct cgcttcgctc tatcgggatg
     5401 ccgagattcg aactcggacc ctacgaaccc gaatcgtaga tactaccgtt atactacatc
     5461 ccgctaaaac cccgacccga cttatgaacg taccgctccg attaacacta atttatcaca
     5521 cctttacctc gttagaagca gaacgcaatt tgattttagc aaacacagag tttaatgccc
     5581 catgtgagct tctaataggg tttacgaatt tattgaaaag agagctatac ttaggggcat
     5641 aattttagac tatatgcccc ctggtgaaaa tttattcagg caagaagggc cggaggttcc
     5701 tgcgcccaaa ccggagcacc agcttgagag ccgtgaagcg acgattgccc gtgtcggcga
     5761 aatattgaag gaaggcattg atgcgcttgt tatccacgga acaacgattc gccgtgacgg
     5821 aaaagaaacg ttcgtactgc ggcccgatct tgacgccatg ggcggattgt atctcatgga
     5881 cattgcaggg atcaactatt ctatggccac gtccgtccca aagggggaaa aaattcctgg
     5941 aaaggttcac attgatactg gaggctatgg ggacacaacc atagttcagg aagacaatga
     6001 ggaagaaagc gtcttctttg atcatcatgc cgaagtaaaa ggagaacaaa cttctgcaac
     6061 caagcttgtg tacgaagcac ttgttgaaaa caaactgctc actcccgaac cctggatgga
     6121 acaatttgtt gaatttgtga cggagtgcga caatctctca tacaaggtca caccaaatca
     6181 ttttaaaagc acgtgggccc attcaatgta cgggcagaat cgttctcttt cagccgagca
     6241 agtagcaaat ctctttaaaa gcggccataa tcctaaaagt gcgtttacac aacaccagcg
     6301 tcaaactatt caagttcttg aaaatcgaag aagagttatg aaccttgaag aggcctgcgc
     6361 aaagcggcag aaagaagtcg ataggtcagt aaaaggtatg gatgtttcca tgaaaaaaat
     6421 gatggcaaac cacttaaaaa tagaaacgcc tgaactcggg aaaattatga tcaatgagat
     6481 tcacagagag ggcaaaaaaa ctatcgacga aatacctctc ggtggaagag cggcaaacgc
     6541 tatgggctat gacacctaca tcatgactga cggcagcgga tttttcatat cctcccaaac
     6601 acacgatctc gaacctatat tcgaaaaaat tcaaaaagaa ttgccggagg cgaagcttgt
     6661 tcgtggaacg atgattcttt ataagtccga aacacatcct gttgcaacac ggcgcttatt
     6721 ggaaatactg aatcttttcg aagacggggc agaacatgac cccgctgtca cctcataggt
     6781 atacttaaac tgaattaatc aaaaaagtac aatactgaaa tatcccagtg aactactttc
     6841 gtgctatagc acaaaagtag ttcactctaa aaaaattaaa attcaatagc ggcgtttaag
     6901 atttacccaa tttagatcta tagcgtttta aaaatgttgg ccactgcgaa tggctgtttt
     6961 ctggaactgt accgagcgta aaccacccga tttcgctaaa tttttctggc tctcctattt
     7021 taactcgcag gcgatccact aacactctgt agtcgagtgc cacccaatgt gttttcctgc
     7081 cgtcgtgttc ccgatgcaca tcccgaaatc ctaaaaattc aaagtcaaga atgtccgtac
     7141 aatattcctc tttgatttcc cgtcgaagcg tatcttcaac cgaatcgccg aattcgattc
     7201 ccccgcctcc gatgtcccac ctgccatgtt catcccgagt attgttattt ctctttgcca
     7261 ttacgaaatt gcccttcccg tcatgacaga agtacacaat acttatacca atgtagtcaa
     7321 aacctttccg catagagacc tattttacta agtgtttact gaaacccaat tgtaccagcc
     7381 gctccgatgc acgccacaca cggtccggca tctcttgcga tatttgaaag cctttttccg
     7441 catagagttt tattttttga gtatctgcga ccatggaatt caaaattcgt tctttgaaac
     7501 cgggggtcat ttctacatat ttttcgtatg aacactgggg cgatgtaacc gtaacctcct
     7561 tttccggcca gagcttctta aacgcggcat acgctctcct ttccatatat ggtttttgaa
     7621 ccactataaa agagccaatt tcaagaccca ttttttcaat caataatttt gtaaaagaaa
     7681 tattttctcc cgtatttgtc gacgtatttt caattataat tgccgattcg ggcacaccac
     7741 gctctctcgc gatatccgca aagacttctg cttcaggttt tttgaaaacc cctctagtaa
     7801 atctgcccac gtttccggag aaaataatgt acggcgcgta tccttcaaaa tagagatcga
     7861 ttccccgctc cgcaaccaaa ggattataac tgccgagcac taatactgcg tcggcttttt
     7921 ttaatgtttg attcaagtgg ttatagtccc acagaatttt tgccagtcga tcaatttcat
     7981 tttcgttttc ttcattcatg ttcgtaaacc gttagaatca gatacgaaga cattaaccct
     8041 cgtgcacact aagactaaag tatacatcgc cgcggtaaac cgagagatat cccgcttcta
     8101 acgcggtaaa tatatcatca ataattgatt tcgtgggctt tttctgtata gtatcgcgag
     8161 acagaaagga gggaattgaa cacttcatct gtacacgtac gacagttttt caaattttgc
     8221 atcgtcgggg cagtgggcat cgcggcatac tacgccattc tcttcgtcct cactgaagga
     8281 gcgcgcgttt ggtatatcgc ctccgcgtgt atcgcgtttt gcttcaatca agtggtcacc
     8341 ttctttttca ataaacggtg gtcattcaag tgcaacgaca aaacagccac cagagcacaa
     8401 atgaggttgt attttgtgat gggggctgta acgcttgttg ggaatgctgc ccttcttgca
     8461 ctgcttgttg aagtttgcaa aatgcactac ctgcgagccc agtttatcct catcgttgtt
     8521 ttttctggaa tcagttactt cttaacccag aaaatctttg cgaaatacat accgccgctt
     8581 taagcgagcg gttttttcat atttcgcccg cgcatgtggc gggtgcgaaa tatgaacccc
     8641 gtaccaatac tagggcaagt gcgcgtgccg aacaggcacg tccctttcgg gcgaagcgcg
     8701 agcccgctcc tcctcttggt gcggggttat tttggccccc agtcttgatg caagaaggta
     8761 aaatttcagt aaattcaaga tagttgaatt ttcgcactca aacgaaaacc aaaacttaaa
     8821 ctcaaacctc aaattcacaa aatattatat agagacgatc gtttctatat aatatttgga
     8881 taagaaattg caaagttaat cctagaatca cgggaagggg cgttatgttt ggtccttgag
     8941 agccgacgac ttcgaaagtc ctaccgcacg atcacgacac ccttgtggga tgcgtccaaa
     9001 tgattgtgat atgcccagtc cccagcttcg ttaaaattaa actcgaaaat tgtctgcccg
     9061 ctgaaaaccg acttgctttg gtcaaacgcg ggaaaatttg tatgcacagg atgaggatct
     9121 gacgctacag agaatgggct tttagtctca tttataaatc tgaccttctg gcctttctga
     9181 actgttattt ctttgggaat aaagccttct tgcgtatagc ggagatttac cgtttccaga
     9241 gtgctcacag agctgtttac atttattccc tctgaagctg tctctcctgg aagttcccca
     9301 cacgccacgt acacatccgg ttctgcttcc gacttatgta cattcactga aagcggtagg
     9361 aacgaaagaa catgcgaaac aaggccgtcg aaattgagcg aatacgatgt tacagacgtt
     9421 ccaccaacaa catttgtgag ctccgacgcg atttcaccta ttttgtcgca cataccaata
     9481 tgaacgtgag caggttgcgg ttcagctgtt ttcttgccgc tcattgtgat agtaattttt
     9541 gctttcccgt cttcctcgct aatcaagact ttgcccattt ggcccgagtt attttgttca
     9601 agcaaatcga attcaatcct tttctctgcg cccgccactg cattttcatc actcttcttt
     9661 tccgacttgc cgcctctata cgccacaatt ccgagtccta caataagcaa gagcaccacg
     9721 acagcgacga tagtgggaag cgatccttta tttaaatttc tcatgaataa aatctaaaaa
     9781 cttgataatt tcaatcaatt cttcataaga gtatcacaaa atactgaccc tcgcgcaaag
     9841 accagaaaca gcatagaaga ccactcgctc tacataaaac agccgagatc gcatcctcgt
     9901 catgcaaatt taatttgcga tatatttttt gttgatattg actgaaacat acccaagacc
     9961 gataaataga aaaccaagaa taacaagcga aatcggccaa ccaagtgaat ctgcaaaata
    10021 tttgcttgtg atgtacgaaa catgagcaat aagaaaaaga gtgctcatga ccaagatact
    10081 gcgacttctc atatacactg acaagacaag acctccaatc acgataagga aatagagcat
    10141 ttgccacgga actgaattaa acacttgtga aaacgcagcg ccaagaaacc cgctaatacc
    10201 aaagaggtaa agaaacccga taagtttctt atttctacca tcacgaaaag catgcccaag
    10261 tagaagatag ctcacgccta ttaccatagt gagatatgca tacaagtcct catgcctata
    10321 aaatggaccg tcaacaatcg cttcaataac aaggtaaacg aaagacgtgc cgtttgcgat
    10381 agcgaaaaaa gctaggatag catttttatg aatagagttt aacaataaat aaaatgccaa
    10441 tatggcgcca aatgtaattg caaccgtcca gtcgccacta tcaagattaa gttcagaaag
    10501 ggtaactacc gcccctccgg gaatcaacat gcccccgatg aagtgaaaaa ctgttccaat
    10561 attgtcttcg ggcttgcttt taagcaacat cgacccagca gccgttataa caaaacctaa
    10621 gcccagcgta accaagatgc gtccaaaaga acctatatca gcccagattt gggaaacaaa
    10681 aataacaata ccggtaacca caattgccgc gcccaaaaca tacaacattt tggtaacgga
    10741 aaaagtcggg gaagttttca aattttccgc cttgacctta ctttgtgttg cagaagcaaa
    10801 gcccaagcga tttaatactt cttcacggcg tatctcgcca gtacttattt tagtagaaag
    10861 ctcttgtagt aattcttcat tattcataaa aaataggcgt gaaaaatgat taatttctgc
    10921 caggtaatac ccacccaaca aacccaaaat tatttctgta ataatactga tcggtatcat
    10981 ttataagatt tattttggtt ttactctttc cttttgtaag gtaataacca tatgatgagg
    11041 aaccgccacc aaaaataaag aagaagtcac caccatttct gtcatagtct cctgaaactg
    11101 taacaccgtc gggtgatgta acaagattat taagtgtcaa agtttgcgcc tcggcgagag
    11161 ttatctctct actctcattc ttttttgtat cgtgcaagaa aatacgatca gtatattttt
    11221 cgttaaaatc tggtacccca tttttgtcga gatcttgagt catgtcgacg ggattaacta
    11281 ctaacttgtt atctacgacc acataacgtc tctggagata gttgtcgcac cggtacggat
    11341 agtaataatt attgccttca gtgcaagctg tataaacaaa gttgtaatca gtggaaagga
    11401 aaagtgatgg aatataagta cttaaagcaa caacggctat gagcatgatt ggcaatataa
    11461 aagcaagaag tatggcgaag tttttcttta gaaattccat ggtcaacatt ataacataag
    11521 caagccgcac attagcgatt ttccgccgaa ggcggatctg cgtcaggcat gacaagtttt
    11581 tctttgcttg cacatccgaa gctttagcga aggagtggcg gctaaagtta aagcggaaca
    11641 attctatttt gttccgtagg aaacgacacg tttctcttaa ggagtatccc taagctttga
    11701 ggataggttg aagcgatatc atccacggga tcgaatcgtt cgatgaaacc attgttatag
    11761 tattcaccgt tttcatcagc ttctgtccaa tagggttcac attctactgt gtatacaaat
    11821 cccttaccag ttcgatctgg tatttcatat ttaacacccc tgtctttaat caacttattt
    11881 gtttctgcta aattctcata atgaaggacg catttatttt taaatgacac ttctcttgaa
    11941 cccaatggta ataactggaa gacatattgt ttgcttatgc aaccaaaggc aaagcagggt
    12001 gtgttgtata agatacctaa aaaagtcttt ttaatagatt ttaatttacc gtcgtcataa
    12061 tcaaaaaaca ctgaagatgt attttcgtca tcagtataaa tgccctcggt agaatccgat
    12121 ttttcataaa cagtgtgtat tccaaatccg aaagaccaca aaacaccaat tacagcaaca
    12181 ccaattagga aaacaattat taaaagtttt ttactcatat gcttatttta ctatatcttc
    12241 catattggcg gtgtatttta aaaatagctc gaacggagtt tgctcgccac gcttcgcgtg
    12301 gctcgtatgg tgggggtgaa taaattcgaa gcggctccgc cgcacattaa caatttaaag
    12361 tttttctttg gcggcaaatt caaaaattcg cgaagcaaat atgtttaaaa tgttcggtga
    12421 gtcaatcaat tttgttccaa aattcttctt agacaagaat cgtacaaaga ctggtcgtat
    12481 ccaccaattc ttgcttgaga aggtggttgc ggacaaagcg aggcatcttt ctttgctata
    12541 gcgatttcgg cgatacaact atcagtggct ggtccaaatg gatggatttt gcacatcgcc
    12601 acatcttgtt tttctatagc cactgtttga acgcatatcc atttattccg atttccgtaa
    12661 tcatcattca aacattttat tccatcatcg ctttggacaa ataaatctaa cgacctcgct
    12721 tgccagtttg tgagcagaat tccattcaga caactttgtc tttcttgcac cgatgtaaaa
    12781 acagaaagtt tatcgcacac agaataatcc ggaataaacc atgatgtgaa ctcattattt
    12841 ttacttggat tgtaaactgc ggttattgta ttttgccacg ctaattcttt gtcagaccgt
    12901 ttaagccaat gggaatagga tgagagaggt acaaataaaa taaggtaaat aaatgtgatt
    12961 attgcttttt gtttccaatt atacttgcga ttaaacaata aaccgatgta aattccaagt
    13021 aatgcgtaga ttaaaaacgc gacaatttgg atagcccacg tagaataatt taatcccaat
    13081 agagctaacg gataatctaa accagaaaaa gataccggaa aagatgcaat gtaaaatcca
    13141 tacggaatga attttgaaag aaatataatc aaagctgcaa ttgcaactga tattac
//
LOCUS       DATDSU010000039        10933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1544760, whole genome shotgun sequence.
ACCESSION   DATDSU010000039 DATDSU010000000
VERSION     DATDSU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544760"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VI981_01255"
     CDS             <1..849
                     /locus_tag="VI981_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478969.1"
                     /translation="TTINLFNTFSQFMFGFQGDKLNTILVSLWPLAVLLAVLAIQKNK
                     RMAPETLYCLISLLVPITLVFVVSILVQPFFVTRYLIITVPALYLVLGWILYTYPKIL
                     RRIVQVGLLVIMLSGVAVQAVGEDVPVKENYREASAYLERRASPQDIVILSAPFTIYP
                     FEYYYAGSADVNTLPIWNRNAVGPIPPFSSEKLPIEVEKLRARHQVAWLLLSYDQGYE
                     ADVKLYFDTHFHRLDQQNFSPGLNLYAYKLRYDNPDVEALINAHASAGSSTETGEISP
                     SESSTE"
     gene            1004..2134
                     /locus_tag="VI981_01260"
     CDS             1004..2134
                     /locus_tag="VI981_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY4478970.1"
                     /translation="MIIETHTYMFPVHSWEFFVMAHEAWDAMYRACSDAKESIDLEQF
                     IFYNDTIGGKFGALMIRKAREGKRVRLLVDTVGSFPFVSSTLLNEMREAGVEISYFNY
                     IKPWKISRIKYWLFRDHRKLLIVDKKIAFMGGVGIGDKLGPWRDTQFSIEGPVVAECV
                     EAFEHVWTTNEQDKFVPFKKPRFVSGGFMLVPHAPHFRQRHIRNTYIDALRSARRYAY
                     ITTPYFAPDRRLFRVMRLAARRGVDVRLLVPEKGDPAFMDKIADAQFGKALRSKIKIY
                     KYTSRMMHAKTIAIDDDWASVGSCNLDDSSLFFNYEITLISVQAKFVETVKRQFEDDI
                     LMSHEYTYDEWKKRSWFQIFLEFYYLYMWRTVFWILKKIFYF"
     gene            complement(2208..2471)
                     /locus_tag="VI981_01265"
     CDS             complement(2208..2471)
                     /locus_tag="VI981_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478971.1"
                     /translation="MARSARKQSTPVTKAPAGFPIRILASFESPNGSIGLQDEPVFLA
                     RRATPEGVPGRWEDHDVTVVRSGSNGNTHFIVKYRDPVPVLQV"
     gene            complement(2589..2942)
                     /locus_tag="VI981_01270"
     CDS             complement(2589..2942)
                     /locus_tag="VI981_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478972.1"
                     /translation="MKIPKLLLSLSCILILASPLVLGFWSLLMASLVYGLALRFLVHK
                     IDMRSQTLQMFAGLIIILCPFVASILHAAYNGPWYAFILFIPSYALMFCGLVAVDKCT
                     KPSVSHGGPIQGATR"
     gene            3109..3504
                     /locus_tag="VI981_01275"
     CDS             3109..3504
                     /locus_tag="VI981_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478973.1"
                     /translation="MHRIIHFFDKLEDRVREALSRKPILYTFIGAFSIVLFWRGVWMT
                     ADSIPFLNGPISIAISVSILLVTGLFVSFFVGDVIILSGVKKEKKIIEKTELEIEQDE
                     ITLRAIKNEIDGIKRDTDKVKKILEQTND"
     gene            3497..4549
                     /locus_tag="VI981_01280"
     CDS             3497..4549
                     /locus_tag="VI981_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4478974.1"
                     /translation="MINKLQINFFYILLIIVFVLTVIIFFPYFEVLFLAIVLAVTFQG
                     FYRHVKKFFGKYDSLAAFVSVIVILVLILLPLTILGFYFFKESRDLYVYLTEQNEAGN
                     SPLYTFMGNIQIYLDRVLPDNLVPESTTADVEQYASKASGWLIGHFQNLFSSVISLVV
                     DLFILLLGLFFFLRDGHKFADIIVKVSPLSDSYDQGIIKKMEIAINSVIRGNILIAIA
                     QGVLATAGFLFFGVPSPILWGSLATVAALVPSIGTAIVILPVSLYLLSAAGTFPAVGL
                     LLWGLLVVGLIDNLLRPFILERGIKIHPFLILLSVFGGIGFFGPIGFIAGPVILSLLF
                     ALVDIYPQVIKAGDRA"
     gene            4553..5125
                     /locus_tag="VI981_01285"
     CDS             4553..5125
                     /locus_tag="VI981_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY4478975.1"
                     /translation="MLDFVTEYKLALLYVGLIVGGTPGLVAAIYFSLVGVYSLTSVFV
                     ACIVTTVAWDGFWYGVGRFIMLERLKKISFLKERLHLIERMEHHYRNHAYKLIFLSRF
                     VYGTNSPISIVSGAHRISFYRFIAVSVGSIGGWFLALSIIGLLLRPSLDEIEGIAKNL
                     TIGFTVFVALMLLVYITVKHLITKRVNKLE"
     gene            5122..5811
                     /locus_tag="VI981_01290"
     CDS             5122..5811
                     /locus_tag="VI981_01290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4478976.1"
                     /translation="MTESKISVIIPAVDEEAFLEKCINSVRMQKKKPCEIIVSINGSK
                     DATLEIAKRMADTVVASTTALGAAEARNLGAAKAVGNILVFLDADCTISENMFEEIEK
                     ILSEGPALGTALAEPDRLHPAALVYIHFKNFLHSTRLHRGAFGMLFCTKEVFDKAGGF
                     SKKLRIGELGEFINRARRAGIPYRVIESGWTTVSMRRLSTRGYLRTMFFWIRWGVAQS
                     PHKKSEIAGEY"
     gene            5814..6704
                     /locus_tag="VI981_01295"
     CDS             5814..6704
                     /locus_tag="VI981_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HEY4478977.1"
                     /translation="MSLFKKKKKVVTHDGSFHPDDVFAVAVISLFEKGCIDIQRTRDE
                     RFLKDADYVVDVGGIYDPDNNLFDHHQPSGAGMRADGSPYAAFGLVWKKFGILLSESQ
                     EAAERIEKRLVEPTDILDNGSSSKKPLQDDIYQYDLSSIIFAFHSTWKEKERSEDDAF
                     FDLVKYAELLLVREIKKMSDKIEGEAKVQEIYEKTADKRIIELDADYSWGDVLKKYPE
                     PLFVITPENGNFHVRAVRDNPNEFKNRCDFPASWAGLRGSELAKITGVPDAVFCHSNR
                     FLAVAKSKAGAETLAELALK"
     gene            6980..7951
                     /gene="obgE"
                     /locus_tag="VI981_01300"
     CDS             6980..7951
                     /gene="obgE"
                     /locus_tag="VI981_01300"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY4478978.1"
                     /translation="MFVDEIKLHLSAGHGGHGVVRWRHEKGKEYAGASGGNGGKGGDV
                     YVRGVRDIGILATYRHKKEFHAERGEDGMKQSMHGKTGRDFCLDVPIGSVITNLISRK
                     KYQILNEGEEVMLLSGGRGGLGNEHFKGSTNTTPFEHTDGEAGEESSFFIELELIVDA
                     GLIGFPNAGKSSLLNALTNASAKVASYAFTTLEPNLGDFYGFILADIPGLIEGASEGK
                     GLGYKFLRHIKRTKLLIHCISLEHENITEAYKTIRAELEKFDKELARKKEILVLTKTD
                     AVDEKTISKKQKEAKKLSKNLFLVSVIDDLRLKQFQDELVKILRADV"
     gene            8018..8599
                     /locus_tag="VI981_01305"
     CDS             8018..8599
                     /locus_tag="VI981_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478979.1"
                     /translation="MQDCVFEHCQFFNKHKIWKTITILGILPEELASRPKGSYDLGEF
                     ARGLLKHGAFPPSTTRGRINLIVLALEELGFTEPAPWARVIRERFLRRWSKRNLRGLL
                     ISHTEPEDAFSLRASYLDQPCDDSQPSNGMHLLKMGMVPLLDLASYPRIFTLERLRTD
                     LPPMIGAHCAGPDCKFGPKEKIVFRIAPIPEAS"
     gene            8742..10205
                     /gene="gatB"
                     /locus_tag="VI981_01310"
     CDS             8742..10205
                     /gene="gatB"
                     /locus_tag="VI981_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY4478980.1"
                     /translation="MSQKYSTTIGLEIHAELKTRTKMFCASKNDPDEKRPNVNICPVC
                     MGYPGTLPVINKQAVAHVLRIGAAVGGKIADFTEFDRKNYFYPDIPKGYQISQYKYPL
                     ITGGSISGVALTRIHLEEDTASSSHEDGSYSLVDFNRAGVPLMELVTEPVIHDAETAV
                     KFAKELQLLLTYLGAGEANMEKGEMRVEANISVAPQRSDLSVDPRSDLVLGTKVEVKN
                     LNSFKAVERAIKYEVERHISLLESGQKITQETRGWDENKQSTFSQRQKEDSHDYRYFP
                     DPDLPKLYLGEIPEFAFVVLKKELPELPWTKRGRFIADYGLKLEDIEIFINDIALASE
                     FEGIVANLEARLPSKRDDLARLASNYITSDFIGIRKNNPAVRLPDDFYELILMVSAGE
                     ISSRGAKDILTIAVQSSEKRTIRNIAKEKGLLQQSDPEALKKMVTEIISDNPTVVADY
                     KAGKIAALQFLVGQGMKKSKGSGNPGVLKKLFEESLK"
     gene            complement(10300..10572)
                     /locus_tag="VI981_01315"
     CDS             complement(10300..10572)
                     /locus_tag="VI981_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478981.1"
                     /translation="MEDHISVMKWLRDRNIPVELFRAFRLELAQAEKEDRVLWKEMGK
                     PKCQRQLSPFLVRHGYKPMHAGRPDTPLTNEDIAMYLKGTNLAAFY"
     gene            10561..10776
                     /locus_tag="VI981_01320"
     CDS             10561..10776
                     /locus_tag="VI981_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478982.1"
                     /translation="MILHHGVSTASQIVQTIWSKAETWFGVELGKSDGRGKNDPSLLF
                     YPRGFIFQEREKSSHEVGDIRSGIWHT"
BASE COUNT         2907 a         2332 c         2648 g         3046 t
ORIGIN      
        1 acaacgatca acctattcaa tacgttttca caatttatgt tcggatttca gggcgataaa
       61 ttgaacacca ttcttgtttc gctctggccg cttgctgttt tgctcgcggt gcttgcgatt
      121 cagaaaaata aacgaatggc tccggagact ctctattgcc ttattagtct cttggtgccg
      181 attacgctcg tttttgttgt ttctatttta gtgcagcctt ttttcgtcac gagatatctt
      241 atcatcactg tcccggcact ctaccttgtc ctcggatgga tactctacac gtatcccaaa
      301 atcctacgac gtattgttca ggttgggctc cttgtcatta tgctctcggg tgtggccgtt
      361 caggctgttg gggaagatgt gccggtaaag gaaaattatc gagaggcgag tgcgtatctc
      421 gagcggaggg cctctccgca ggatattgta attctctcgg caccctttac tatttatcca
      481 tttgagtatt actacgcggg cagtgccgat gtaaatacac tgcccatttg gaacagaaat
      541 gctgttggcc caatacctcc gttttcaagc gagaaacttc cgattgaagt tgaaaaactt
      601 cgggctcgcc atcaagtcgc gtggcttttg ttgagttacg atcaggggta tgaagcggac
      661 gtgaagcttt attttgatac tcattttcat cgattagacc aacaaaattt ttcccccggg
      721 ctcaatctct atgcgtataa gttgcgatac gacaatcctg atgttgaagc gcttattaat
      781 gctcacgcgt ctgcaggttc ttccaccgaa acgggcgaaa tttcacctag tgagtcgtct
      841 accgagtaaa gcgaagcata aaacgtcgca tcaacgcaat aatactcgtc gtgatttcat
      901 acttttatgg tcgtcgcttt tagggtaaag tagggagata attttcaagt cttggcattt
      961 aagacctgcg gtatgttaac cgatatccgc ttgtaaccag tatttgataa ttgaaacaca
     1021 tacttatatg tttcctgtcc actcttggga attttttgta atggcgcatg aagcctggga
     1081 tgcaatgtat agggcctgtt cggacgctaa agaaagcatt gatcttgaac aatttatttt
     1141 ctataacgac acgattggag gaaagttcgg cgcactcatg attagaaaag caagggaagg
     1201 taagcgcgtg cggcttcttg ttgatacagt tggaagtttt ccgtttgttt ctagtactct
     1261 tcttaatgaa atgcgtgaag ctggggtgga gatcagctat tttaattata ttaagccgtg
     1321 gaaaatttca cgcataaaat actggttgtt tcgcgatcat aggaaacttc tcatcgtcga
     1381 taaaaaaatt gcttttatgg gcggagtagg aataggggat aagcttggtc cgtggagaga
     1441 tacgcagttc tctattgaag gtccggttgt ggccgaatgc gtcgaggcgt ttgagcatgt
     1501 atggaccaca aatgagcaag ataaattcgt cccatttaaa aaaccgcgct ttgtgtccgg
     1561 cggctttatg cttgtgccgc acgcgcctca ttttagacag cgccacatcc gaaacacgta
     1621 tattgatgcg cttcgaagcg cccgccgata cgcttatatc acaactccgt actttgcccc
     1681 tgaccgaagg ctttttcgag tgatgcgcct tgccgcgagg cgcggcgttg atgtccgtct
     1741 tctcgtgccc gaaaaaggag atcctgcatt tatggataag atcgccgatg cgcagtttgg
     1801 caaggcgctt cgctccaaaa taaaaatata caaatatacg tctcgtatga tgcatgcgaa
     1861 gacgatcgcc attgacgacg actgggcgtc ggttggtagc tgtaatttgg atgactcgag
     1921 tctgtttttt aattatgaaa tcacgcttat ttcggttcag gcaaagttcg ttgaaacagt
     1981 taagcggcaa tttgaagacg atattctaat gtcgcatgaa tatacgtatg acgaatggaa
     2041 aaaaagatcg tggtttcaga tttttcttga gttttactat ctgtatatgt ggcgcaccgt
     2101 tttttggatc ttgaagaaga ttttctattt ctgatagcca gatttctaga aaacaaaaaa
     2161 agcccgcgtt cagtggggct ttgtgtttcc aaaaagtgct cggtcggcta tacctgcagc
     2221 actggcaccg gatcacggta cttgacgatg aagtgggtgt tcccgttgga accgctccga
     2281 acgacggtta catcatggtc ttcccatcgg ccgggcaccc cttccggagt tgctcgcctc
     2341 gcgagaaaaa caggttcatc ttggagtccg atacttccgt ttggactctc gaagctcgcg
     2401 agaattcgga tcgggaatcc cgcgggagct ttggtgaccg gtgtgctttg ttttcgtgct
     2461 gaacgtgcca taggttgtgt tttgtggtta tgtgacgcga ctccgaaaat agattaacct
     2521 aagtagaaca agcagtcaag cgtacaaaaa aagcgactga ttgtcgcttg gggaaaactt
     2581 ccggcgtcct agcgcgtggc accttgtata gggccgccgt gtgatacgct tggttttgtg
     2641 catttgtcca cggcaacgag gccgcaaaac atcagggcgt acgacggaat gaacaagata
     2701 aacgcgtacc acggaccatt gtaggcagcg tggagaatcg acgcaacaaa agggcaaagt
     2761 attatgatga gtcccgcaaa catctgcaga gtttgcgacc gcatgtcgat tttgtggacc
     2821 aaaaagcgaa gtgccaatcc gtacacaagc gaggccatga gcaggctcca gaagcccaaa
     2881 acgagtgggc ttgcgagaat gagaatacac gaaagagaga gcaatagttt ggggattttc
     2941 atgtctttgt tttggtaagg ccgaaacact gggcgcagtt ttgcgaacgt taatgtgccg
     3001 accggaaaca agtttagtct aggatgagca atttgtctat ttgtctatcg atcgggagcc
     3061 cactgtcttt tcgttgagag agcaaaaatc caatgctata ctctatgagt gcaccggata
     3121 atacactttt tcgacaaact ggaggatcgc gttcgcgaag cgctttcgcg caaacccatt
     3181 ttatatacgt tcattggcgc cttttcgatc gtcctttttt ggcgaggtgt atggatgacg
     3241 gcagattcaa tacctttttt gaacggtccg atttcgattg caatcagtgt ttcaatcttg
     3301 cttgtgacag gccttttcgt gtctttcttc gtgggtgacg tcatcattct ttccggagtg
     3361 aaaaaagaaa agaaaattat tgagaagacc gaacttgaga ttgagcagga tgaaatcaca
     3421 ctccgcgcta tcaaaaacga aatagacggt ataaaacgcg acacggataa agtaaaaaaa
     3481 atactcgaac aaaccaatga ttaataaatt gcagataaac ttcttttaca ttcttcttat
     3541 tatcgttttt gtgctaacgg taatcatttt tttcccgtat tttgaggtac ttttcctggc
     3601 aattgttctg gctgtaacgt ttcagggttt ttaccgtcac gtcaaaaaat tttttggaaa
     3661 gtatgattct cttgctgcat ttgtatccgt tatagtgata cttgttctga ttctactccc
     3721 actcactatc ctcggtttct atttctttaa agagtcgcgc gatctttatg tatatctcac
     3781 ggagcaaaat gaagccggaa acagcccact ttacactttc atgggtaata tccagatata
     3841 tctcgatcgc gtcttgcccg acaatctggt gcccgagtca actactgccg atgtcgaaca
     3901 atatgccagc aaagcttcag ggtggctgat tggccatttt caaaatctct tttcaagtgt
     3961 catatcgctt gtcgtggatc tttttattct gctcctcggt ttgttttttt tccttcgcga
     4021 cgggcacaaa tttgcggaca tcatcgtaaa agtcagcccg ctttcggata gctacgacca
     4081 gggcatcata aagaaaatgg aaatagccat aaactctgtt attcgcggca atattctgat
     4141 cgctatagcg cagggagtgc tcgccacggc cggctttttg tttttcggtg ttccgtcgcc
     4201 cattttgtgg gggtcactcg cgactgtggc agccttggtt ccaagcatcg gaacggctat
     4261 tgttattttg cccgtgtcgc tctatcttct ttctgccgcg gggacatttc ctgcagtcgg
     4321 gttattgctt tggggacttc tcgtggtcgg tttgatcgat aatttgctcc ggccgttcat
     4381 tcttgagcga ggaatcaaaa ttcatccgtt tctcatactg cttagtgttt tcggcgggat
     4441 cggttttttc ggtccgattg gctttatcgc agggcctgta attttaagtc ttctctttgc
     4501 gcttgttgat atctatcctc aagttattaa agctggagat cgcgcttagt tcatgcttga
     4561 ttttgtcact gaatataaat tagcactttt atacgtaggt ttaatcgtgg gcggaactcc
     4621 cggcctcgtt gccgctatct acttttcatt ggtaggtgtc tattcactca cgtcggtgtt
     4681 tgttgcctgc attgtcacga ccgttgcctg ggatggcttc tggtatggtg ttggtaggtt
     4741 cattatgctc gaacgtctca agaaaatttc ttttttaaag gagcgtctgc acctaatcga
     4801 aagaatggag catcattacc gaaatcatgc atacaaattg attttcctat cgaggtttgt
     4861 gtacggcaca aacagtccta tatcgatcgt cagtggagct catcgcattt ctttctatcg
     4921 gtttattgct gtttctgtag gaagcatcgg cggctggttc cttgctcttt cgataattgg
     4981 cttgctattg cgtccgtcgc ttgacgagat cgagggcatt gcaaaaaatt taacaatagg
     5041 ttttactgta tttgtcgcct tgatgctact cgtctatata acggttaagc atttgatcac
     5101 gaaacgtgta aataaactcg aatgaccgag tcaaaaattt cagttattat ccccgcagtg
     5161 gatgaagagg cctttttgga aaaatgcatt aactcggtca gaatgcagaa gaaaaagccc
     5221 tgtgaaataa tcgtctccat aaacgggtcc aaagacgcga cgctagagat cgcaaaacgc
     5281 atggctgata cagttgtcgc ctccacaact gcactcggcg cggccgaagc gcgaaatctg
     5341 ggcgccgcaa aggcagtggg aaatatactt gtgtttctcg atgccgactg cactatttca
     5401 gaaaatatgt tcgaagagat tgagaaaata ctgtccgaag gcccagcgct tggtacagct
     5461 ctcgccgaac ccgacaggct tcatccggcg gccctcgtat atatacattt caaaaatttc
     5521 ttgcactcca cacgccttca tcgcggtgca ttcgggatgt tgttttgcac aaaggaagta
     5581 tttgataaag ctggcggttt cagtaaaaaa ctgcgaatcg gtgagctcgg tgaattcata
     5641 aaccgagcaa gacgagcggg aatcccgtac agggttattg aaagcggatg gactactgtc
     5701 tcgatgcggc ggctctctac tcgtggctat cttcgaacga tgtttttctg gatccgatgg
     5761 ggagtggccc aaagtcccca caaaaaaagt gaaattgccg gagagtatta atcatgtctt
     5821 tatttaagaa aaagaaaaaa gtcgtaactc acgatggcag tttccatcct gatgatgttt
     5881 ttgctgttgc tgtgattagt cttttcgaaa aggggtgcat agatattcaa agaacgcggg
     5941 atgaacgatt cctcaaagac gcggattatg ttgtggatgt cggtggaata tatgatcctg
     6001 ataataattt gtttgaccac caccaaccga gcggtgctgg aatgcgtgcg gatggaagtc
     6061 cctatgctgc attcggcctt gtgtggaaga aattcgggat cctgctctcg gaaagccagg
     6121 aggcggccga gcgcatcgaa aagcgcctcg tcgaaccaac cgatatttta gataatggct
     6181 caagctcaaa aaagccgctt caagacgata tttatcaata tgacctttct tcaatcattt
     6241 tcgcctttca ttcgacgtgg aaggaaaaag aaagaagcga ggatgacgcc ttttttgatt
     6301 tggtgaaata tgccgaatta ctgctcgtac gcgaaataaa aaaaatgtcc gataaaatcg
     6361 aaggtgaagc aaaagtgcaa gaaatatacg agaaaaccgc tgacaaaaga atcatcgaac
     6421 ttgacgccga ctattcttgg ggagacgttt taaaaaaata tccggaaccg ctttttgtta
     6481 tcacgccgga aaatggaaat tttcatgtgc gggctgttcg tgataatccg aatgaattta
     6541 aaaatagatg tgactttccg gcgtcgtggg caggtttgcg cggctcggag cttgcaaaaa
     6601 tcacaggcgt gccggatgca gttttttgtc atagcaatcg ctttcttgct gttgcgaagt
     6661 caaaggcggg ggcagagact cttgccgagc ttgccctcaa gtgatatttt agattaggag
     6721 atcctgccta tgactaatcg aaaaaaagaa actgtgcggc ttcagttgcc gccaactcgt
     6781 ggattgcgcc tcggattgtg tcgcggcata cctgttaccg tggatactct tatgcggcgg
     6841 caaggactat atacgtacct acgttggcgg tttggcaaga cgcgtcgaaa aatacaaaga
     6901 cttctgcaaa cctgacggtt tgcttttttt gttgctattc cattctaaaa gctataagct
     6961 gaaacttaaa ggctaatata tgtttgtcga tgaaattaaa ttacatttaa gcgcaggtca
     7021 cggcggccac ggcgtcgttc gttggcggca tgaaaaaggc aaggaatacg ccggtgcttc
     7081 gggaggtaac ggaggaaaag gaggcgatgt atacgtacgc ggagtacgtg atattggcat
     7141 tctcgctacc tatcggcata aaaaagaatt tcacgccgag cgaggagagg acggaatgaa
     7201 acaaagtatg cacggcaaaa ctggccgcga tttttgtctc gatgttccga tcggttcagt
     7261 gatcacaaat ctcatttcgc gaaaaaaata tcaaatttta aatgagggcg aggaagtgat
     7321 gttgctctct ggcggccgag gaggtctcgg taacgagcat tttaaaggtt ccactaacac
     7381 gacgccgttc gaacacaccg acggagaagc gggcgaggag tcaagttttt tcatcgagct
     7441 tgaacttatt gtggacgcag gattgatcgg ttttccgaat gccggcaagt cgagcctgct
     7501 gaatgcgctg acgaatgcga gcgcaaaagt ggcatcgtac gcgtttacaa cgcttgagcc
     7561 gaatctcggc gatttttatg ggtttattct tgcggatatt cccggattga ttgaaggtgc
     7621 gtcagagggc aaaggtcttg gctataaatt tctgcgtcat attaagagaa ctaaacttct
     7681 gattcattgt atttcccttg aacatgagaa tattacggaa gcgtataaaa caatacgcgc
     7741 cgaacttgaa aaatttgata aagaattggc aagaaagaag gaaattcttg ttctgacaaa
     7801 aacagacgcg gtggatgaaa aaactatctc caaaaaacaa aaagaggcga aaaaactctc
     7861 aaagaatctt tttttagtct ctgtaattga cgatcttcgg ttgaagcagt tccaagacga
     7921 actagtcaag attctccgtg ccgatgtcta gcttgtagaa tagcctttcg tcatgtatcg
     7981 ttgagtctga accaccctaa acccggatct tctttctatg caagactgcg tattcgagca
     8041 ctgccagttc tttaataagc acaaaatttg gaagacaata accattctcg gcattcttcc
     8101 ggaagaatta gcgagtcgtc ccaaaggaag ctatgatctc ggagagttcg cacgcggact
     8161 tttgaagcat ggagcctttc caccgagtac gacgagggga cgaatcaacc tcattgtgct
     8221 tgcactcgag gaactcggtt ttacagaacc ggcaccttgg gcacgagtta tccgcgagag
     8281 attccttcgg agatggagta agagaaactt gcgcggtctc ctcatttccc acaccgagcc
     8341 tgaagatgcg ttttcacttc gagcaagcta ccttgaccaa ccatgcgatg attcgcaacc
     8401 gtcgaacgga atgcaccttc ttaaaatggg catggtacct ctgcttgatc tggcttcata
     8461 tccgcgcatt tttacgcttg aacgactgcg gactgatctt ccgcccatga tcggggcgca
     8521 ctgtgccggc cctgattgta aatttggtcc gaaggaaaaa attgttttcc gaattgctcc
     8581 aatccccgaa gcttcctagt gaagcttttt taattgtcgg tttatagtta tactactttt
     8641 tatctggata tgagcgaaaa atacataact accataggtt tggagattca ccccagtagt
     8701 aaaaccggag cggtttacta cggggcaagt gccgcacttg tatgagtcaa aaatactcca
     8761 ccacaatagg cttagaaatt cacgccgagc tcaagactcg gacaaagatg ttttgtgcgt
     8821 cgaaaaatga ccctgatgaa aagcggccga atgtgaacat ttgtccggtg tgtatgggat
     8881 acccgggaac acttcctgtt ataaacaaac aagccgtcgc gcatgttctt aggattggag
     8941 cggcggtcgg aggaaaaatt gccgatttca cagaatttga tcgaaaaaat tatttttatc
     9001 ccgacatccc aaaaggctac cagatcagcc agtacaaata tccgcttatt acaggtggct
     9061 ctatctctgg agtggcgctc acgagaattc atctcgagga agataccgca agctcatctc
     9121 acgaagacgg aagttatagt cttgtcgatt tcaacagggc gggagtaccg ctcatggaac
     9181 ttgtgaccga acccgtaatt cacgacgctg aaacggcggt caaattcgcg aaagaattac
     9241 agttgttgct tacgtacctt ggtgccggag aagcaaatat ggaaaaaggg gaaatgcgag
     9301 tggaggctaa tatttccgtt gcgcctcaaa ggtcggacct ttctgtcgat ccaaggtccg
     9361 accttgttct cggaacgaaa gtcgaagtca aaaatctaaa ctcgttcaag gcggtcgaac
     9421 gtgcaattaa atacgaagtg gagcggcaca tctctctttt ggaatcagga cagaaaatca
     9481 cgcaggagac gcgcggatgg gatgaaaaca agcagtcaac attttcacaa cgccagaagg
     9541 aggattcaca tgactaccgt tactttcccg atccagatct gcctaagctc tatcttggtg
     9601 aaattcctga atttgcgttc gtggtattga aaaaagagtt gcccgagttg ccgtggacga
     9661 aaagaggacg atttatagca gattacggat tgaagttgga agacatagag atttttataa
     9721 atgacatcgc cttggcttct gaatttgaag gaattgtcgc caacttggaa gcaaggcttc
     9781 cgagtaaaag ggacgatctt gcaagacttg catcaaatta tataacttca gattttattg
     9841 gtattcgtaa aaataatccc gcagtaagat taccggacga tttttatgaa ctaattctta
     9901 tggtttcggc aggagagatt tcgtcacgcg gagctaaaga tatattgaca attgccgtac
     9961 agtcgtctga aaaaagaaca atccgcaata ttgcaaaaga aaaaggcctg cttcagcaaa
    10021 gcgatccgga agcgctcaaa aaaatggtaa cagaaattat ttccgacaat ccaacggtag
    10081 ttgccgacta caaagcgggt aagatcgctg cgcttcagtt tctcgtcggt caagggatga
    10141 agaagtcaaa aggttccggc aacccgggcg ttttgaagaa attgttcgag gaaagtttaa
    10201 agtaaaaaac aattttgtat tattctaagc tactcgcaca ttcgcacgaa tgtgcaagta
    10261 tggaaacgtg aaatggtaaa aaaaaccgag cgaactcggt tagtagaatg cggcgaggtt
    10321 ggttccttta aggtacatcg cgatgtcctc gttggtcaag ggtgtgtctg gacgccctgc
    10381 gtgcataggc ttgtatccgt ggcgcacgag gaatggactg agttggcgct ggcatttagg
    10441 tttgcccatc tctttccaga gcactcgatc ctccttttcg gcttgcgcca gttctagtcg
    10501 aaacgcgcga aacagttcga ccggaatgtt ccggtctctc aaccacttca ttacacttat
    10561 atgatcctcc atcacggcgt ttctaccgct tcccaaatag tacagacaat ttggtccaaa
    10621 gctgaaacct ggttcggagt ggagcttggg aagagcgacg gacgaggaaa gaacgatcca
    10681 tctctccttt tttacccgag ggggttcatt tttcaggaaa gggagaagtc gtctcatgag
    10741 gtgggcgata ttagaagtgg tatttggcat acgtagaatc tccttccact aaaatactga
    10801 aaaaatctgc attgtcaaga gtcgcgggat gagaccggtg acataggagt ttgcagatgc
    10861 tacattactt ttcggtaagt tgctcatcca agcatgttgt tttaccttca cctctcgacc
    10921 ttgaattgga aag
//
LOCUS       DATDSU010000040        14492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2209847, whole genome shotgun sequence.
ACCESSION   DATDSU010000040 DATDSU010000000
VERSION     DATDSU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14492
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209847"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..792
                     /gene="rpsB"
                     /locus_tag="VI981_01325"
     CDS             82..792
                     /gene="rpsB"
                     /locus_tag="VI981_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY4478983.1"
                     /translation="MEAKKEKNSELVEAMFKVGAHFGFGRSRRHPSVKSYLFGAKNRV
                     EVFDLEKTSDLLIRAKEFITKIVSTGGTVLFVGGKNESRDIVKNTAEELGVPYVAGRW
                     IGGTLTNFANIRKRIEKMEDLISKREKGELSKYTKKERLLIDREIENLKRFFLGLSRL
                     KDYPHTLFIIDHKREHIAVAEARSRGIPVVSLCGSDSNIKEVDYPILANDTSLSSIKF
                     FVGEIASAVRAGKATPKA"
     gene            834..1415
                     /locus_tag="VI981_01330"
     CDS             834..1415
                     /locus_tag="VI981_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY4478984.1"
                     /translation="MEISTQQIKELRELTGISIMQCRKALEEAGGDREKALVILRKKG
                     GEAVGKKADRTLAAGSVHSYIHSNGNIGAMIELKCETDFVSKNPEFKALAYEIAMHVA
                     ATNPEYLKIEDIPADAKATAREVFEKDLAGKPKNMHENILEGKLSSYFNERVLLMQPF
                     IKDADKTIANLLEAGVQKFGEKIEITRFVRFSI"
     gene            1419..1805
                     /locus_tag="VI981_01335"
     CDS             1419..1805
                     /locus_tag="VI981_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478985.1"
                     /translation="MFFESYVFIIALGVIGMLVVHKIWEMRHGASVVPNVLIEKSDGL
                     VNATLGASEGAFWERIERAFIGAIRMIEHVSRAIALLIVHRIHGWSSALLDKLRSKHL
                     KRNRNSVSFPLKHIGEDLKKQEENKM"
     gene            1857..1932
                     /locus_tag="VI981_01340"
     tRNA            1857..1932
                     /locus_tag="VI981_01340"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1890..1892,aa:Thr,seq:tgt)
     gene            2030..3568
                     /locus_tag="VI981_01345"
     CDS             2030..3568
                     /locus_tag="VI981_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4478986.1"
                     /translation="MFNGFKDTSYFHIGSARDLKGKDRVLYRTLEIFPGLLTWSTIIG
                     VILFSYKAPLFVAVFIIIFDTYWLLKTAYLSIYLRQSWRRLRYNIGVDWTRRIDLIKY
                     DHIFHLVILPFYDEPEKVAEESIQGILAARWDPKKIIIVLASEEKAGESAEVTSHNLK
                     TRYGNLFFDFLVTRHPADIPSEIAGKGSNITYAAKAARILLTSHGIRPHDVLVSAFDI
                     DTVPYVHYFECLTWHFLTHADRFRASYQPVPFYNNNIWQVPAISRVVATSGTFWQMIQ
                     QERPDRLATFSSHAISMATLEKINYWQTNIVSEDSRIFWNALMFHAGNYEVVPLSYPV
                     SMDANVGTSFWQTIKNVYKQQRRWSWGVENVPYAMFLFIKSPQIGMLKKIKYMFALVE
                     GFWSLATNSLVIFILGWLPIIIGGGEFHDTVLSFNLPIITRNLMWLSMFGLVLSAIIS
                     LSFLPAAPDGYKPRHRFYMIIQWLLVPLTLTLFGAIPAIDAQTRLMFGKYMSFWVTPK
                     HTRF"
     gene            3616..4473
                     /locus_tag="VI981_01350"
     CDS             3616..4473
                     /locus_tag="VI981_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478987.1"
                     /translation="MKPPSQVNRRFYLFFFTLLFLVAVPIIVPFAVGYRLDKNFKLVA
                     TGGIYVNGIARDSEVFLNGKRVKDSRFYDQGFFVQNLRPDTYFIIVARSGTWSWSKKL
                     EVKEGKVTLAHAVQISQKPTVREVVPLLSAATSTRSSEYERVSALFNTESAITFLRAT
                     SSLPLGSEANPILKRKVSLWKKGSVLYAGWTGESENVPYFFCASECSARTPIFVRPYE
                     LGGIDFMPGRFDVALVKLEDGIYAIELDKRSEQNTIPLYMSATADYRVVNGETLYIKD
                     GKKLFEYVE"
     gene            4505..5206
                     /gene="pheS"
                     /locus_tag="VI981_01355"
     CDS             4505..5206
                     /gene="pheS"
                     /locus_tag="VI981_01355"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY4478988.1"
                     /translation="MTEHGHIHPLTSTMSEIVSIFSELGFEVAEGPEIESEFYNFDAL
                     NMPADHPARDMQDTFWVIPLSKRQCLRTHMTSVDVGRFLETHKNDLPARVIAPGKVFR
                     NEATDSTHEAQFFQIDGVYVDASVSLGDLMGTLEYCFKKLLGDDVKIRFRPSFFPFVE
                     PGVEVDIEFRGKWLEVMGAGMIHPNVFKTVGVDTSKWRGFAFGGGIDRLVMIKYDIPD
                     VRYFYNGDLRLVNQF"
     gene            5208..7151
                     /gene="pheT"
                     /locus_tag="VI981_01360"
     CDS             5208..7151
                     /gene="pheT"
                     /locus_tag="VI981_01360"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY4478989.1"
                     /translation="MRFSHSWIQSYFDEKIPAPDKLAEIFTFRFSEVEAIEKIGDDAI
                     LDVKILPDRACYALSHRGVAREVSALCGMPVKKSEAPTLHHLDNGLLKISVEDTKLCL
                     HYVGTLMDGVTINSSPEWLQKRLNSIGQRSINVIVDIANFLMLDMGQPLHAFDADKVI
                     GTLCVRVAKKGEEMTTLDEKHVLLQGDELIIADREGPLGIAGIKGGKRAAVTEKTKRL
                     ILEAALFDATHLRKISTRIGIRTDASRRFENRIPYELQAETMDRFVALIQETTKAHAV
                     SYSDVRGEPIIEPHIVVSIDYINSILGTKLQAVDIEKIFNSLSIEFSRESGNYRLSVP
                     TWRLDLNIPEDIAEEVGRLYGYENIEHTRLKSDLKKPHVLKSLAIANKVRLLLAEWGF
                     SEVYTPTMVASGEVEIENPLADDKKMLRSDLTENIRTSLDFNLNNTPILGTEIVRIFE
                     IGKVFPKNGEHTSLAIGVKAPKSLKKVNVNEEIRNVRENLIKALGLPLGTVCTIDDSG
                     GLLLIDERAAGEINKEDGIMELNFDMLVAVMPDQEAYPKLFLRETKFKAYSPYPFIVR
                     DIAVFVPENTEAAELEKILLKNSTELLVNHRLFDEFRKGGEKSLAFRLIFQSHERTLT
                     DKEVNDIMKVTTVEIANKGWKVR"
     gene            complement(7356..7661)
                     /locus_tag="VI981_01365"
     CDS             complement(7356..7661)
                     /locus_tag="VI981_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478990.1"
                     /translation="MVTLDNSLTIDPYRYLGHLMTFMNDGKECTGIITQGYRDEGGLR
                     FTIRTQQGSVRKHVPLWSIGLLRKKAERVTIGFDNGEILSIIPSKSPFSEETRALAN"
     gene            complement(7752..8072)
                     /locus_tag="VI981_01370"
     CDS             complement(7752..8072)
                     /locus_tag="VI981_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478991.1"
                     /translation="MKKQQNGTWALVAPKGTSLHTTKDHADILLIKVGRKSATGEPVP
                     RSVLEEWGMDGDGVEWRITVKYSNTVSDLLDALERLGQLSRGHGDLMETVKVFAIEST
                     ETRK"
     gene            8261..10342
                     /locus_tag="VI981_01375"
     CDS             8261..10342
                     /locus_tag="VI981_01375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HEY4478992.1"
                     /translation="MAKDKNKTKAKKSTYSAESITVLEGLEPVRKRPGMYIGTTGPDG
                     FHHLVTEIFDNSRDEAMGGFANDIEIALLPGEIVRVVDNGRGVPVDMHKQTKVSALET
                     IMTTLHAGAKFGGEGYKVSGGLHGVGASVVNALSSYCKVIVHKDDGIYVQEYSRGKRK
                     AAVKKVGSSKLTGTIVEFQTDNEIFGAQKFDFHRITTHMRQQAYLVKGLRISVMDLRD
                     YKGSIDADDVFYARELAIDGPSMTFYFEGGLVSLVKFSNQLQKPIHKNIFYVEKIGGD
                     IEAVEIALQYVDDISARILPFANNIYNGEGGTHLTGFKTALTRSLNTYCRKNGMVKES
                     EDNFTGEDVLEGLTAVISIKMREIQFEGQTKAKLGSVEAQSAVATVFSESFQAFLEEN
                     PDDARAIVQKVILALKARKAAKAAKDSVLRKGALEGMTLPGKLADCQTRDASLSELFI
                     VEGDSAGGTAKTGRDRRTQAILPLRGKILNIERARLDKMLGSEQIKNLVVAMGTAIGD
                     TFDITKLRYHKIIIATDADVDGAHIRTLILTLLYRYFKPLIDGGYVYIAQPPLYKIKR
                     GKEIFYAYSDQEKKKIVGKEVDDLEMVEQAAEEAEQTEEREVDGEPEKEKTEPKQPKI
                     SIQRYKGLGEMNSEELWETTMDPERRILKQVKIEDAAEADKVFDMLMGTDVPARKAFI
                     QSNAKHANIDI"
     gene            complement(10587..10829)
                     /locus_tag="VI981_01380"
     CDS             complement(10587..10829)
                     /locus_tag="VI981_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478993.1"
                     /translation="MKNIPVHIDPRTTTFRKINRKLVSGITAEHFEEQFTGRYVSPGV
                     SVREYVAMMRAGDVLLESQTVIKLKDPVPDSFLNFC"
     gene            11104..11286
                     /locus_tag="VI981_01385"
     CDS             11104..11286
                     /locus_tag="VI981_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY4478994.1"
                     /translation="MFSNYNSIAIKNTFEASDECAGINIVVIDDVTTTGATLDEAHRT
                     LIEAGATKVTCIALAH"
     gene            complement(11576..12151)
                     /locus_tag="VI981_01390"
     CDS             complement(11576..12151)
                     /locus_tag="VI981_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY4478995.1"
                     /translation="MISISPSRLSVGGFTFIELLVVVSIISLFASIFIASFNSTRGKA
                     KDGMVQNYMAQLRTVAENNHLAGVYSSVTFQRVPYESGITPPDCVIQTGFEAIESNLV
                     KLDQNIRVMQGVDCATTNAEKGINIIKQTGAGADTSYFAYARLPSTENFTGIARAWWC
                     IDSSGNSKKVTNLGGTSIPPAPPNDVTALCP"
     gene            complement(12289..13371)
                     /locus_tag="VI981_01395"
     CDS             complement(12289..13371)
                     /locus_tag="VI981_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478996.1"
                     /translation="MELPNQKPMPPEAPKHAGDVEKVKEVTVKTLAVLGLIAILSLFT
                     YGIVKYAPIAVKTIARSAVSLSSKFSGSNSDTLTVLSDKKKIDSGEAFTLTWTNKDTA
                     EGSYSLSYPCQNGVHLESLDRDTAEVVFCNTNFRFASESNSITIAAFSTKLDSTNLPI
                     SLNFTRTDDRAPAFEAKAKITITNSDAGSSVEPTPTPTPTPAPRPTPRPTPQAGPQTQ
                     NSYPIPSTGSTISDPNGYVDLSARIIATGYIDQTTNAFVPAPETRIRQKGAVKFAVEN
                     VGTKVSPVWRFNAVLPTYPSFIFHSEDQINLLPGDRIEFVLGFDKIAYQNENTVTINV
                     DPTSSINEKSKDNNIVRANIHVNLSN"
     gene            13657..14316
                     /locus_tag="VI981_01400"
     CDS             13657..14316
                     /locus_tag="VI981_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY4478997.1"
                     /translation="MFKTILQAYIEIIRNMKLFVVLIVCTFSITIGVLAAFGFVPESP
                     LLRDEVKASVHESEKPRVASYAVQTPEFPTHVKIASIGIDTPIVNPTTFDTNLLDAEL
                     QKGAVRYPGSAKLNENANIFLFGHSSGLPVVRNRAYKAFNNLGKAEIGDEVIVTSATH
                     EYVYRVVSVSHKKDSEAYVTFSTGKKMLTLSTCNTFGKKQDRDIVEAEFIKSYPLESS
                     Q"
BASE COUNT         4097 a         2992 c         3415 g         3988 t
ORIGIN      
        1 cggtttgcaa aaggatgctt aatttgctag actccactcg cgtgcctgta aacaggcctt
       61 gttttgatta aaaattgact aatggaggca aagaaagaaa aaaacagtga gctagtagaa
      121 gcaatgttta aagttggcgc tcattttggc tttggacgat cacgtcgtca tccttccgta
      181 aaatcctatc tttttggtgc taagaacaga gtagaagtat ttgatcttga aaaaacaagc
      241 gatctcctta tacgggcaaa agagtttatt accaagatcg tcagcaccgg cgggaccgta
      301 ttgtttgtgg gcggcaaaaa tgaatctcgc gacattgtaa aaaatacggc cgaagagctc
      361 ggcgtgccat atgttgctgg acgatggatt ggcggcacat tgacgaattt tgctaatatc
      421 agaaagcgaa ttgaaaaaat ggaagatctt atttcaaaac gagaaaaggg agagctttca
      481 aaatacacaa aaaaagagcg attattgatc gatagggaaa tcgaaaattt gaagcgtttc
      541 tttttgggat tgtcccgtct caaggattat ccgcatacgc tttttattat cgaccataaa
      601 cgagaacata ttgcagtcgc agaggctcgc tcaagaggaa ttcccgttgt cagcctgtgc
      661 ggttcagaca gcaacataaa agaggttgac tatccgatac tcgctaacga tacatcgctt
      721 tcaagtatta aattttttgt cggtgagatt gcgagtgcag ttcgagctgg caaggcgact
      781 cccaaagcgt agtctgcgga gcgtcggcat ttatattttg cactttatat actatggaaa
      841 ttagcacaca acaaataaaa gaattacgag aactcaccgg tatttctatc atgcaatgcc
      901 gcaaggcgct tgaggaggcg ggaggagata gggaaaaggc gcttgtaata cttcgaaaaa
      961 aaggtggcga agcggtcggg aaaaaagcgg atcgaacgct tgcggccggc agtgtgcact
     1021 catatattca ttcgaatgga aatatcggtg ccatgattga actcaaatgt gaaacagatt
     1081 tcgtgtcaaa gaacccggag ttcaaggctc tcgcatacga aatagctatg catgtcgcgg
     1141 ctacgaatcc tgaatatcta aaaatagaag acatacctgc cgacgccaaa gctacagcgc
     1201 gggaagtttt tgaaaaagat ctcgctggta aaccaaaaaa tatgcacgaa aacatactcg
     1261 agggtaagct gtcttcatat tttaatgagc gggtgctgtt gatgcagcct tttataaaag
     1321 atgctgataa aacgatcgca aatcttctag aagccggagt tcaaaaattc ggggaaaaaa
     1381 tcgaaatcac tagattcgtt cgcttttcta tatagtatat gttttttgaa tcatacgttt
     1441 ttatcattgc tctcggggtt atagggatgc ttgtcgttca taaaatctgg gaaatgaggc
     1501 acggcgcttc cgttgttccc aatgtattga tcgaaaaaag tgacggtctt gtcaatgcga
     1561 cactcggtgc aagcgagggc gctttttggg agagaatcga gcgcgcattt attggtgcta
     1621 tacggatgat cgagcacgtt tcgagagcga ttgcactttt gattgtacac agaattcacg
     1681 gctggtcttc ggctctttta gataaacttc gttcgaaaca tttgaaaaga aatagaaatt
     1741 cagtttcttt tcccctgaag cacattgggg aggatttgaa gaaacaagag gaaaataaaa
     1801 tgtaattatt gcctcggcta ggagtttttg atagtctgta gtcttcgaaa tatattgccg
     1861 ccttagctca gttggtagag caccactttt gtaaagtggc tgtcctcggt tcgagtccga
     1921 gaggcggctc cagtgttgta gaaaaagatg cagaagtact aagcgcatgg gacttcctag
     1981 cgaatttttg ttacagtatg gtcacgcgga attgtaaaga tactccctaa tgttcaatgg
     2041 tttcaaggat acatcatatt ttcacattgg ttctgcacgt gatctaaagg gaaaagacag
     2101 agtattgtac cgaacactcg aaatttttcc aggacttttg acatggtcta cgattattgg
     2161 cgttatctta ttttcctaca aggcacctct ttttgttgct gttttcatta ttatttttga
     2221 tacctattgg cttttaaaaa cagcgtatct ttcgatttat cttcgtcaga gctggagaag
     2281 actgcgctac aatatagggg ttgactggac tcgaaggatc gatctgatta agtacgatca
     2341 catttttcat cttgtgatcc tgcctttcta cgacgagcct gaaaaagtgg ccgaagaaag
     2401 tatccaggga atattggccg ctagatggga tccgaaaaaa ataattatcg ttcttgcgag
     2461 cgaggagaaa gcgggggaaa gtgccgaagt gacatcccat aaccttaaga cacgttacgg
     2521 aaatcttttt tttgattttc tggttacgcg tcatcctgca gatattccga gtgaaattgc
     2581 cggaaaaggt tctaatatca cttacgcggc caaagcggca cgaatacttc ttacttcaca
     2641 tggaattcga ccgcacgatg tgcttgtatc agcttttgat attgataccg ttccgtatgt
     2701 tcattatttt gagtgcctta cgtggcactt cctgactcat gcggatcgct ttcgcgcctc
     2761 ataccagccg gttccttttt acaacaataa tatctggcaa gttccagcga tatcgagagt
     2821 tgtggcgact tccggtacat tttggcagat gattcaacag gagcgtcctg accggctcgc
     2881 aactttttcg tcgcacgcca tttccatggc gacactcgag aagataaatt actggcagac
     2941 aaatatagtt tccgaggact cgcgcatttt ttggaacgca ctgatgttcc acgcggggaa
     3001 ttatgaagta gtgccgctgt cgtatccggt ttctatggac gcgaacgtag gaacatcttt
     3061 ttggcagact ataaaaaatg tctataaaca acagcgtcgc tggagctggg gtgtcgagaa
     3121 tgtcccgtat gcgatgtttt tattcatcaa aagtcctcaa ataggaatgt tgaaaaaaat
     3181 caaatatatg tttgcactgg tggaaggttt ttggtcactt gcaactaatt cgcttgttat
     3241 ttttatattg gggtggcttc cgattatcat tggcggcgga gaattccacg acaccgtact
     3301 ttcttttaat ctgccgataa ttacgagaaa tctcatgtgg ctgtcaatgt tcgggcttgt
     3361 gttgtctgct attatttcgc tttcgttcct gccggcggcg ccggatggtt acaagccgag
     3421 gcaccggttt tacatgatta ttcaatggtt actcgtaccc ctgacgctca cgctctttgg
     3481 ggcgattccc gctattgacg cccaaactcg tctcatgttc gggaaatata tgagtttctg
     3541 ggttactccc aaacatactc gcttttaaac tttcaagttt ctacgttaca ctggtagctt
     3601 acttaaaaca aatccatgaa gccgccgtct caagttaata ggcgttttta tctctttttt
     3661 ttcacacttc tatttttagt agctgtcccg attatcgtgc cttttgcggt ggggtatcgg
     3721 ctcgacaaaa attttaaatt agtggccact ggggggattt acgtaaacgg gatcgcgcgt
     3781 gattcagaag ttttcttaaa cggtaaaaga gtaaaagatt cgcgatttta cgaccaaggt
     3841 tttttcgtgc aaaacctgcg ccccgacacc tattttatca tcgtagcccg tagcggtacg
     3901 tggtcttggt ctaaaaagtt ggaagttaaa gaaggaaaag tgacgctggc tcacgccgta
     3961 cagatatcgc agaagccaac agtacgcgaa gtcgtccctc tgctatctgc ggcgacctca
     4021 acacgctctt cggaatatga acgagtcagc gctctcttca atacggagtc ggcaataaca
     4081 tttcttcgtg cgacgtcgtc tctaccctta ggttctgaag caaatccaat tcttaagcga
     4141 aaagtttcac tgtggaaaaa aggaagtgta ctctacgccg gctggacggg agaaagtgaa
     4201 aatgttccat attttttctg cgcctctgaa tgcagcgcgc gcacccctat atttgttcgt
     4261 ccgtacgaat tgggcgggat tgattttatg cccggcaggt ttgatgttgc gcttgtaaaa
     4321 ctggaagacg ggatctatgc cattgaactc gataaacgat cagagcaaaa tacaataccg
     4381 ctgtacatgt ctgcgacggc tgattataga gtcgtaaatg gcgagacgct ttatataaaa
     4441 gacgggaaaa agttattcga atacgtcgag tagataagtc tgctttatcg tataatgtaa
     4501 atctatgact gaacacggcc atatacaccc ccttacatcg acgatgagcg aaatcgtgtc
     4561 catttttagt gagcttggat ttgaagttgc ggaaggaccg gagatagagt ccgaattcta
     4621 caattttgat gctttaaata tgcccgcaga ccatcctgct cgagacatgc aggacacttt
     4681 ttgggtaata ccactgtcta aaaggcagtg tctgcgtact catatgacct ccgtggacgt
     4741 cggacgtttt ctagaaactc acaaaaatga tctgcctgct cgggttatag cccccggaaa
     4801 agtcttccgg aacgaggcga cagactccac acatgaggcg caattctttc aaattgatgg
     4861 ggtatatgtc gatgcgtccg tgtcgcttgg cgatcttatg ggcacgctcg aatattgttt
     4921 taaaaaacta ttgggggacg atgtgaaaat acgttttcgc ccgagttttt ttccattcgt
     4981 cgaacctgga gttgaagtgg atatcgaatt tcgaggcaaa tggcttgaag tcatgggggc
     5041 agggatgatc cacccaaacg tttttaagac agtcggagta gacacctcaa aatggcgcgg
     5101 atttgcattt ggcggcggta tcgatcgcct tgtgatgata aagtacgaca ttccggatgt
     5161 ccgttatttt tacaatggtg atctacggct tgtaaatcaa ttctgatatg aggttttctc
     5221 actcctggat tcaatcatac ttcgatgaaa agattcctgc tccggataaa ttggcagaga
     5281 ttttcacgtt cagattttcg gaggttgagg ctatcgagaa aattggagat gacgcgatac
     5341 ttgatgtaaa aattctcccg gacagagcct gctatgcgct ttcacaccgt ggagttgcac
     5401 gtgaggtgag tgctctttgc ggaatgccgg tcaaaaaaag tgaagcacca acgctccatc
     5461 acctcgacaa cggcctgctc aagatatcgg ttgaagatac gaagctctgc ctccactatg
     5521 tgggcacgct catggacgga gtaactataa actcgtctcc ggagtggctt cagaaaagac
     5581 taaactcaat aggccagcgt tctatcaatg tcattgtaga tattgctaat tttctgatgc
     5641 ttgatatggg gcaacccctg catgcttttg acgcagataa agttataggc actctttgtg
     5701 ttcgcgtcgc aaaaaaaggc gaggaaatga cgacactcga tgaaaagcac gtactgcttc
     5761 agggcgatga acttatcatc gccgatcgcg agggaccact tggaattgcc ggtatcaaag
     5821 gaggaaagcg cgccgctgtc acagaaaaaa cgaaaagact gattcttgaa gcggcgcttt
     5881 tcgacgccac gcatctacgc aaaatatcca ctcgaattgg tatccgtacc gacgcatctc
     5941 ggcgctttga aaatcgtatt ccttatgaac ttcaggcgga gacgatggat cggtttgtgg
     6001 cattgataca agagacgacc aaagctcacg cggtttcata cagtgacgtt cgtggagaac
     6061 cgatcataga gccacatatt gttgtctcga ttgattatat taattcaatt cttggcacaa
     6121 aacttcaggc tgttgatatc gaaaagatat tcaactcact ttcaatcgaa ttctcgcgtg
     6181 aatcgggaaa ttatagactg tcagtaccta catggaggct tgatttgaat atccccgaag
     6241 atattgctga agaagtcggc agactttatg gctatgaaaa tattgagcac acgcgtctaa
     6301 agtctgattt aaaaaaacct cacgttttga aatctctcgc catcgcgaat aaagtaagac
     6361 tgttgcttgc cgagtgggga ttttctgaag tgtacactcc gacaatggtc gcgagtggag
     6421 aggtggagat agaaaatcct cttgctgatg acaagaaaat gcttcgtagt gatctcacag
     6481 agaatatccg tacctcactg gacttcaatc tcaataacac accgatctta ggtaccgaga
     6541 tagttcgtat ttttgagatc ggtaaggtat ttcctaaaaa tggagaacac acatcactag
     6601 ctataggcgt aaaagcgcct aagagcttaa aaaaggtaaa cgtcaatgaa gaaattagaa
     6661 acgtgcgaga aaacctgata aaagcactcg ggcttccgct cggtacagtg tgcactattg
     6721 atgacagcgg cggattactt ttgatcgatg agagagccgc cggcgaaata aacaaggaag
     6781 acggcattat ggaattaaac ttcgacatgc tggtagccgt aatgcccgat caagaagcct
     6841 atccgaagtt atttttgcga gagacaaagt ttaaggcata ttcgccctat ccttttattg
     6901 tccgtgatat cgccgtattt gttcctgaaa atacggaagc ggcagaattg gaaaaaatac
     6961 ttctaaaaaa ttccacggag cttttggtaa atcatcgttt gtttgatgaa tttagaaaag
     7021 ggggagagaa atctttggcg ttcaggctca tttttcaatc acatgagcga acgctgacag
     7081 acaaagaagt aaacgacatt atgaaagtga ccaccgttga aattgcaaac aaaggctgga
     7141 aggtccgatg aaatatttat tttggctagc aaggagcggg tgtccgcctt aggtggacac
     7201 aacgtccgag cgaagacaaa atatgggtca ataccgcgtg gcttgccacg ctttagggcg
     7261 agcgaagcgg gcaccaagta ttagcctgcg gcttgccgca ggggcctata tttgcagtca
     7321 ttaaaaaaac cgaagcagtg cttcggcgaa gtggttcagt tggcaagagc gcgtgtttcc
     7381 tctgaaaaag gagatttcga tgggataatg ctcaagattt caccattatc gaagccgata
     7441 gtgactcgtt cagccttctt ccgaagaaga ccgatactcc agagcggaac gtgctttcga
     7501 acggatccct gctgtgttcg aatggtgaaa cgcaaccctc cttcatcgcg atatccttgc
     7561 gtgatgatcc cggtgcattc ctttccatca ttcatgaagg tcatgagatg ccctagatac
     7621 cggtacgggt caatggtgag cgaattatcg agtgtaacca tgcgattcct ttcttctctt
     7681 gatgtttttg gtgtccagcc aattactttt tacacccaca tacgttcaaa gtcaatgaaa
     7741 aaaacgcgaa attattttcg cgtctctgtt gattcgattg caaatacctt caccgtttcc
     7801 ataagatcac catgaccacg tgagagctgg ccaagcctct cgagtgcgtc aagtaggtcg
     7861 gatactgtgt tgctgtactt cacggtaatc ctccattcca cgccgtctcc atccataccc
     7921 cattcttcga gcacactgcg aggaaccggc tctccggtgg cggatttccg ccccactttg
     7981 atgagtaaaa tatccgcatg gtcctttgtg gtgtgcaatg acgtgccctt cggtgcgacc
     8041 agtgcccagg tgccattttg ttgttttttc atagaagtag cgtttggttt gttgccgtcg
     8101 aaactgtact aagaatatcg cactatgtca aaattctacc gagaaaatga tcctctttta
     8161 tccacattca aataatggaa aaagctagat tttatcaagc tattttggta taattcatca
     8221 attaaaagca atcagtgctt tttaaaattg cggcagaaaa atggctaaag acaagaacaa
     8281 aacgaaggct aaaaaatcga cctacagcgc ggaatcaatc acggtgcttg aaggcttgga
     8341 accagtcaga aaacgcccgg gaatgtacat tggcacaact ggtcctgacg gctttcacca
     8401 cctggtaact gaaatctttg ataactcgcg tgacgaagct atgggcggtt ttgccaatga
     8461 tatagaaatc gcgctcctcc ctggcgagat agtgcgagtc gtagataacg gccgaggtgt
     8521 tcctgtcgat atgcataaac aaacaaaggt ttccgcgctt gagaccatca tgaccacgct
     8581 ccatgctggg gctaaatttg gaggtgaagg ctacaaggtg tccggaggtc ttcacggagt
     8641 cggtgcgtct gttgtaaatg cactttcctc atactgtaaa gtaatagtgc ataaagacga
     8701 cggaatatat gtgcaggaat attcgagggg aaagagaaag gctgccgtaa aaaaagtggg
     8761 atcgtcaaaa ctgacgggta cgatcgttga attccaaact gacaatgaaa tctttggcgc
     8821 gcaaaaattt gattttcatc gcatcactac ccacatgcgt cagcaagcat atctcgtaaa
     8881 aggacttcgt atttctgtaa tggatcttcg agattacaag ggaagtattg atgcagatga
     8941 tgtgttttat gcgcgagaac ttgcgatcga tggcccgtcg atgactttct attttgaggg
     9001 cggtcttgtt tcgcttgtca aattctcaaa ccagcttcag aaaccgattc ataagaatat
     9061 tttttacgtc gagaagatcg ggggagatat tgaggctgtt gaaattgccc tgcaatatgt
     9121 cgatgacatt agcgcccgca tccttccttt tgctaataat atatataacg gagaaggtgg
     9181 cacgcattta accggtttta aaactgctct gacaaggtcg ctgaatacgt attgccggaa
     9241 aaatggcatg gtcaaggaga gcgaagacaa ttttaccggt gaggacgtgc ttgaggggct
     9301 tactgccgtt atttccataa agatgcgcga aattcagttt gaaggacaaa caaaagcaaa
     9361 actcggctct gtggaagctc aaagtgccgt cgctacggtg tttagcgagt catttcaggc
     9421 atttcttgag gaaaatccgg atgatgcccg cgcgattgtt cagaaagtta tattggcgct
     9481 taaagcgaga aaggcggcca aggctgcaaa agacagcgtt ttgcgcaagg gtgcgctcga
     9541 aggaatgaca ttgcccggca aattggccga ctgtcaaacg agagacgcat ctctatcaga
     9601 attgtttatt gtagagggag attctgccgg tggtacggcc aagactggcc gggaccgcag
     9661 aacgcaagcc atattgcctc tacgaggaaa gatcttgaat attgaaagag ctcggctaga
     9721 caagatgctt ggttcagagc agataaagaa tttagtcgtt gctatgggaa ctgcaatcgg
     9781 agacacgttt gatattacaa agcttcgtta tcacaaaatt attatcgcaa cagatgccga
     9841 cgtagacggc gctcacattc gcacgctcat tcttacgtta ctttaccgat acttcaaacc
     9901 tcttattgat ggaggctatg tctacatcgc ccagccgcct ttatataaga taaaaagagg
     9961 aaaggaaatt ttttacgcat attcagatca agaaaagaaa aaaatagttg gcaaggaagt
    10021 tgatgatctt gaaatggtag agcaagcggc ggaagaggct gaacagacag aagaacgcga
    10081 agtagacggc gaacccgaaa aggagaagac agaaccaaag caaccaaaga tttccattca
    10141 acgctataaa ggtcttggag aaatgaactc cgaggaactc tgggagacaa caatggatcc
    10201 ggagagaaga atattaaagc aggtgaaaat agaggatgcg gctgaagcgg acaaggtatt
    10261 cgacatgctc atgggtactg atgttccggc tcgaaaagca tttattcagt cgaacgcgaa
    10321 gcatgcgaat atagatatat gaccgctcgg attgtctttt ataaaacacg tataaaattc
    10381 tgctgaattt ttactatgct cgccccgcgt ggcggggctg tgcaatgttt tataaaagac
    10441 aatcctcgct acgacggtgg ggagtaattc ggaggcatca cgcctcgtat taaacctcga
    10501 tggaaagcct gccttataca aaaaaaccaa ggcctggcct tggcggaagt tgatttgccc
    10561 tagtaaaaaa agctcgagaa gaacagctag caaaaattga ggaacgaatc tggtactgga
    10621 tctttcagtt ttataacggt ttggctctca aggagtacat cgccggcccg catcatggcg
    10681 acgtattctc gaacactgac tccgggagaa acatagcgcc cggtgaattg ctcctcaaag
    10741 tgttcggcgg tgattccaga aacgagtttc cggttgatct tgcggaatgt tgtggtacgt
    10801 ggatctatat gaacagggat atttttcatg tcttgctaca gcatatcaca aaatatttcc
    10861 cactttatcc aaggtcggac cttgaaaggt tatgaatttt acctcttttc tcgcctgctc
    10921 ctcaagattt ctaaaagtgc taatgacttt cccttggatg accacacttt taacaaaaaa
    10981 atgttttagt ttaggatttg cgggttgtag tctaattcta ttcttttctc tataaatctt
    11041 tttaagagta acctcgttac cgtttatcaa agcaacggtg gtctctccat cttctactgt
    11101 attttgtttt ctaattacaa cagtatcgcc atcaaaaata ctttcgaggc atccgacgaa
    11161 tgcgcaggca taaatattgt agttatagat gacgtgacca caacaggtgc cactttagac
    11221 gaagcacatc gtaccctaat tgaggccggt gccacaaaag ttacctgtat tgcacttgcg
    11281 cattaggatt cagtatttaa ggactgtaag ctgaaataga agggagacga tgggataaat
    11341 ctgacctcat aaatataacc gattgccagt cctatcaatt ccacggcaga gatcattcct
    11401 gaatgtgctc ataactcatt tatatggcac gtaatttgct atatggcagt atgagtctca
    11461 tgtttatagt aaggcattat ttattgggta acttgtattt atctatttca gcttaacttc
    11521 attgaagcct ccgcgggttt acgcccgggg tcttttgtga aggtggataa aaatgctatg
    11581 gacataaggc ggtgacatcg tttggcggag cagggggtat gctagtacca ccaagattag
    11641 taactttttt tgaattccca ctgctatcaa tgcaccacca cgcgcgagcg atgccggtaa
    11701 agttttcggt tgacggtagt ctagcatagg caaaataact cgtatcagcc ccagcgcctg
    11761 tctgtttgat aatgttaatg cctttttcag cgttggtggt ggcacaatcc acaccctgca
    11821 tcactcgtat attctgatca agtttgacca agttgctctc tattgcttca aacccagttt
    11881 gaatgacgca atcgggcgga gtgatgccac tttcatacgg aactcgctga aacgttactg
    11941 atgaatatac ccccgccaga tgattgtttt cggcaacggt cctcagttga gccatataat
    12001 tctgaaccat cccgtcttta gccttgccac gagttgaatt aaaagaggca ataaaaatgg
    12061 aagcgaaaag actgataata gagacaacaa ctagaagctc gatgaaagtg aatcccccca
    12121 cactaaggcg cgaaggagag atcgatatca taaaatttgt tagctctaga ctagcaaact
    12181 gctagtaatt acttttaata agtcaagtat atcatttaaa aagtccacgc atgaaaacaa
    12241 aaacacccca cttcggggtg tttttgtact gggaaacagg actgcgcttt agttgctcaa
    12301 atttacgtga atattggcgc gaacaatgtt attgtccttg ctcttttcgt tgatagagct
    12361 tgtcggatcg acattgattg ttaccgtatt ttcattctgg tacgcaattt tatcgaagcc
    12421 aagcacaaac tcgatgcgat ctcccggaag aagattgatc tggtcttcgg aatggaagat
    12481 gaacgaaggg taggttggca agactgcgtt gaatctccac accggcgaaa cttttgtccc
    12541 gacgttttcg acagcaaact ttacggcgcc tttttgtctt atcctcgttt ctggagccgg
    12601 aacgaatgcg ttagttgtct gatcaatata accggtggca atgatacgcg cactcaaatc
    12661 tacgtatcca tttggatctg aaatcgtcga tcctgtgctg gggattggat aagaattctg
    12721 ggtttgcgga ccagcttgcg gagtaggacg aggggttggc cttggtgcag gtgtcggtgt
    12781 aggagtcggc gttggctcta cagaagaacc tgcgtcactg tttgtaattg tgatcttagc
    12841 tttggcttcg aatgccggag ctctatcgtc tgttctcgta aagttgagag atatcggcag
    12901 attagtgcta tccaactttg tcgaaaaagc agcgatcgtt atcgaattac tctctgatgc
    12961 gaaacggaaa ttggtgttgc aaaaaacaac ttcagctgtg tctcggtcaa gtgactcaag
    13021 atggactcca ttttgacatg gatagctcaa gctatatgat ccttctgcag tgtctttatt
    13081 ggtccatgtg agtgtgaatg cttctcctga atcgatcttt ttcttatcag ataaaacggt
    13141 cagcgtatcg ctatttgaac cgctaaactt tgacgacagt gacacagctg aacgcgcgat
    13201 cgtcttcacg gcaattggcg cgtatttaac gattccatag gtaaagaggg aaaggatggc
    13261 gatcaagcca agaacagcca gtgtcttcac tgttacttcc ttcacttttt ctacgtcgcc
    13321 tgcgtgcttg ggggcttcgg gcggcattgg tttttggttt ggtaattcca tatataatca
    13381 cgtacggggt gttagaacct catactattt attgctagta aatagtcaat accatgggca
    13441 ttgacaactg tcaatcttcg taatacagta gagttacaaa aagtaaattg attcagacgg
    13501 attataaacc agtgtgacaa cattgtcaat aggcaactat tgacacattc gactaaactc
    13561 agtgtcaatc ctctgctgtg tcgaaggatt gacaaaacag cttctagtgc tagagtcaaa
    13621 acgggaagtc agactccagt tctctatatg agagcgatgt ttaagaccat tctgcaagcc
    13681 tatatcgaaa ttattagaaa catgaagctt ttcgtggttc ttatcgtatg cacgtttagc
    13741 attactatcg gcgtattggc ggcatttggt ttcgtgccag agagcccgct tcttagggac
    13801 gaagtaaagg cctccgttca cgaatcggaa aagccaaggg tagcatcata tgctgttcaa
    13861 actccggaat ttccgacgca cgtaaaaata gcgtcaatag gcatcgatac tccgattgtg
    13921 aatcccacta cgtttgatac gaatctgtta gatgcagaac ttcaaaaggg tgctgtgcgg
    13981 tatccgggtt cagcaaagct caatgagaac gcgaatattt ttttgttcgg gcatagttcg
    14041 ggactacccg tagtccgaaa tagagcgtac aaggcgttca acaatcttgg aaaagcagag
    14101 atcggtgacg aagtcatcgt gacatcagca actcacgagt acgtctacag agttgtttct
    14161 gtgtctcaca aaaaagacag cgaggcatat gtgacgttta gcactggcaa aaaaatgctc
    14221 acgctttcaa catgcaacac attcggtaaa aaacaggatc gcgatatcgt tgaagcagaa
    14281 ttcataaaaa gttatccatt agagtctagt caataaaata tgaattatca gttaaaacgt
    14341 gaaggtatga aaaagatatt tgacatttgc acggtgtttg tttttgcggt gctcattttt
    14401 gcgggtatcg gtactttgcg cgcacatgct agttcaaaga ctcccaagac acctgtcgag
    14461 gtatgcgatc ttgaaatttc aaaatcagtt ga
//
LOCUS       DATDSU010000041         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1713884, whole genome shotgun sequence.
ACCESSION   DATDSU010000041 DATDSU010000000
VERSION     DATDSU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713884"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..1298)
                     /locus_tag="VI981_01405"
     CDS             complement(528..1298)
                     /locus_tag="VI981_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4478998.1"
                     /translation="MEYTRKKDVFELTIDGKIKKYPIIELPRAFVEWNLGQRVGFLEH
                     MKDPAGHGAMRAPAFGAHLPVYITKNSEETLFPANAATKGTGFLPKKEYIEYYIEKFR
                     KIMDDAGTKIEKSEAALVEAKLKHMEAIREFYSDTEKIDLRCLAGLEIWPGVTTKNFR
                     NDPRVSLHFLGMPSGHKKPMRYQQWQINCIWEEIKAGDPRFDFGSTLRELTLGKVGKS
                     FVPGHIPVEHTPIRGKYPSGWILWVIEAFDKGLNSTGV"
     gene            1489..1968
                     /locus_tag="VI981_01410"
     CDS             1489..1968
                     /locus_tag="VI981_01410"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY4478999.1"
                     /translation="MDVNRLVREYLEGSNLMQIATCAKNIPWVCTVCFAVDEKLNVYW
                     FSSHETRHSQEIILNSRVAVAVVQPHTLGNPVRGLQFSGLASELKNPDEIRHSLSCNA
                     KRYGVTAQKTRALQAELESGQAKYGIYRIKPDSFLLYDTLNFPKSPQQKLDLNDTLI"
     gene            2470..2730
                     /locus_tag="VI981_01415"
     CDS             2470..2730
                     /locus_tag="VI981_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479000.1"
                     /translation="MQEKNDAAATACEVDDQLCEMARWTIQKGLARDNFEANFFLVAI
                     FIVITITTFLLAEATFKKETDTGAIRSFIEDAKADTLNSNAF"
BASE COUNT          843 a          684 c          701 g          868 t
ORIGIN      
        1 caaaagaaaa gtgtccgaag gaaaatcaaa aagccaagct cttgtttgtc ttcgtcgtca
       61 aatggttaat attgtctgga tgatgttgaa acacaaaacc atttacagaa atgcttgact
      121 ttttctcata gcggatcttt ctgctttttt gcccacagct tcagagaaaa atgtgggcgt
      181 ataatgtcga agcaaaaatt ataaatgtgt atgcgtgaag tagggtgagt ccgcgcggtg
      241 cggatgattc tctcactgac gcgcaacggt aaaccccgta gtagaaattg gcatcgcact
      301 tcgtgccgct aggcataatg ccaattttca ctacggggta aagtccgatc ccatgcaatc
      361 ccgccagtgg cgggagtggc tcggtcaggc attttccttg tattaccctg cggcttgccg
      421 aaggggccta tatagacgag gcggaggaac attaaatgtt ccgtggttta cggcaacata
      481 acctagccca tcgggaaaat cgcccttttt cagtttaggc tcctctatta aacacccgta
      541 ctattcaaac ctttatcaaa ggcttcaatt acccacagta tccagcctga tggatacttt
      601 ccccggattg gcgtatgctc gacgggaatg tgccccggaa caaatgactt tcccactttg
      661 cccagggtca actcccgaag cgtacttcca aaatcaaaac ggggatcacc tgctttgatt
      721 tcctcccaga tgcaatttat ctgccactgt tggtatcgca tcggtttttt gtgaccggac
      781 ggcataccga gaaaatgaag ggaaactctt ggatcgtttc tgaaattttt ggttgttact
      841 ccgggccaaa tctccaatcc ggccaagcat cgcagatcaa ttttttccgt gtcgctgtaa
      901 aattctctga tggcctccat gtgtttaagt tttgcctcca ccaacgccgc ttctgatttt
      961 tcaattttcg tgcccgcatc gtccatgatt ttgcggaatt tttcgatgta atactcaatg
     1021 tattcttttt tgggtagaaa gcctgtgccc ttggtggcgg catttgccgg aaaaagtgtt
     1081 tcctcgctgt ttttggttat atacaccggc agatgggcac caaaagccgg cgctctcatc
     1141 gctccgtggc ctgccggatc tttcatatgt tcgaggaagc cgacgcgctg tcccaggttc
     1201 cattcgacaa atgcccgcgg taattcgatg atcggatact ttttaatttt gccgtctatc
     1261 gttaattcaa aaacatcttt ttttctagta tattccatgt gcatcattat cgcgagagtg
     1321 cagagtgcag tcaagaattt tgtggggaac tatttttgac atgttataac cctgaagtag
     1381 atcttgaaac atgtaagact tgagccgcgg gttgttatag taagaatatc agaaaatgtc
     1441 cacaagctct gcgtggggag tgagctcact tcagggtata attcgcgcat ggacgttaat
     1501 cgtctggtac gagaatacct cgaaggctca aacctaatgc agattgccac atgcgcgaag
     1561 aacattccat gggtttgcac cgtttgtttt gccgttgacg agaaactcaa tgtatactgg
     1621 ttttcaagtc acgaaacgcg gcactcacaa gaaattattt taaattccag agtcgctgta
     1681 gccgtcgtcc aaccgcatac tcttggtaat ccggttagag gactgcagtt ttcgggatta
     1741 gcgtctgaac tcaagaatcc tgatgagata cggcattctc tctcatgcaa tgccaagcga
     1801 tacggagtaa cggctcaaaa aacaagggct ttgcaggccg agcttgaatc cgggcaggca
     1861 aaatacggaa tttaccgaat caaacctgat tcatttttac tgtatgatac tttgaatttt
     1921 ccaaaatctc cgcagcagaa attggatcta aacgacacgc tcatctaagg ccgggaattg
     1981 gaggtcattc tctcgagatt gataaggaag tcgggttatg ggcttgtcgg acgtgctctc
     2041 aaagatcgag gcgaaacctt gctggcgtaa gaatacggta gacaaggcgt ttgcgatcga
     2101 atctcgtggg gcattcctta aaacctcgaa actagacatt ttcctatttt atggtattgt
     2161 gaaccaatgc atctaaagca taggaacaga cgtcttacat ggtcggaacg gcccaggaaa
     2221 atggagattt ttttgtgtgt ctgcgggcgc aaattcatca aatcgaggaa acgccaagat
     2281 gagtgtctct tttgcatcta taaaaagaaa aactagctag agcgacactt taagcagtgt
     2341 cgcaagaatt acagctaaaa tataggcccc tgcggcttgc cacagaggcc caaagttacg
     2401 aaaaatgaca gaaaaatgtc cacatgttcc aagggagaag tgagcccact ttggggtata
     2461 atcattgtca tgcaagaaaa aaatgacgcg gccgctaccg cgtgcgaagt tgacgatcaa
     2521 ctttgcgaaa tggctcgctg gacaatacaa aaaggcttgg cccgagataa tttcgaggcg
     2581 aatttttttc tcgtggcgat ttttatcgtt attacgataa cgactttttt attggctgaa
     2641 gctactttta aaaaagagac tgatacaggt gcaattagat cgtttatcga agacgcaaaa
     2701 gcagatacgt taaattcgaa cgcattctaa aatcacacgt tcccgtcact ttttcggtca
     2761 gagcaaagaa tctggggttt accctgaagt gagctcattc aatacaatga atttatggtc
     2821 aatttcttac ttaaaacaaa acaccgagtt ccgcagagga agcaatctgt ttcaagatct
     2881 acttcagggt tcacccggtt agtagaagat ggcgtcgcgc ccaaagattt gcgggtattt
     2941 ttttccaaaa ttttcaactt gccaatattt ctgttattta gatacatgca aaaagggcgc
     3001 aaaaaatcaa cgccatcttt cactaccggg ttcacgctta tcgaagtgct tattatgctc
     3061 gccattatcg gacttgtggg tgcgatcgct ctttct
//
LOCUS       DATDSU010000042         5640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2046259, whole genome shotgun sequence.
ACCESSION   DATDSU010000042 DATDSU010000000
VERSION     DATDSU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046259"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VI981_01420"
     CDS             complement(<1..1281)
                     /locus_tag="VI981_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY4479001.1"
                     /translation="MARNSVDGGQIIYLVDTGVIKELKFPTFWFEPTPQGLFMLQVAF
                     GQSKYFSDNLVENVKRGIRQKIRRGEWPGQKPFGYVYDHRLRNIVPEPKEAKIVKKIY
                     EEFATVRHTLKSISARLAELGGGKARSNYSIENLLTNDRYIGIMRWNGETHEGKYRPL
                     ISKQLFAKVQEVLANRRKPRKSKKKHDFPFVGLFNCSCGSMITAQWTHGNGGTYRYYR
                     CTRKHGACSEKYVQENDLKRKIIERSRAIALPADWAHELYAMLETEEKKGAQSATTSA
                     EAISKKMFLLDEKLDKLVTGYLDNLIDEDTYRKKKGELVEQKISLKNEKELLLRKWMS
                     GWIEPTRDYIKTLCQAEKIASAESLGEISQFAQKIGTNRLISEKIASWDFAPPLDFTA
                     QFLASPASRRDEQTIALASRKSRSPIWCAWMDSNH"
     gene            1548..1892
                     /locus_tag="VI981_01425"
     CDS             1548..1892
                     /locus_tag="VI981_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY4479002.1"
                     /translation="MNGPIYEQSVIVGVDAHKYSHTAAAVDAWGQERGRLNFTNDSLA
                     DCLSWLKTLGRREDVIVAVEDANSYGCHLVSALAGQKFAIRSVPAVLTERDRQHSTQR
                     DKSDFWTSRLPK"
     gene            complement(2254..3243)
                     /locus_tag="VI981_01430"
     CDS             complement(2254..3243)
                     /locus_tag="VI981_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4479003.1"
                     /translation="MMQNKDMYGESSKITPSLRNTALQDLIHSYEVRYRFSIGFRTSL
                     ISDRQEVFSWQSDIRRPAASLMKLFVLGALAEEIASENYDARQKLTVHTFVGGSGLLK
                     YVGAPIALTLEELAYFMLAYSDNSATNQLIELLTVDRVNEFINKIGASNTKMIVPMMP
                     TKARGLKDYNYTTADDILLFYKGLSNGFPKSIHLGCAPLCRKFFAVSQPSFLNTMTFF
                     ASDAQMRCLKGVARHNFLSLGKYVYALLSTFRGKRLTKNVAHNRIMAQKSATDKTIFH
                     DFCAVRLDNDFLCVVCMIQCDEGNFYKPLTKRYRAARRLLRRIGKIALNEIHQ"
     gene            3510..3722
                     /locus_tag="VI981_01435"
     CDS             3510..3722
                     /locus_tag="VI981_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479004.1"
                     /translation="MSNISKNIRKMREMKGLSQEKLARLADVANNTIVKIEAGKNQNP
                     TLDTLNKIAKALEVTIDDLANNKYAK"
     gene            3712..5055
                     /locus_tag="VI981_01440"
     CDS             3712..5055
                     /locus_tag="VI981_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY4479005.1"
                     /translation="MPNNLPKVVGTDDIIKLRNGKKARVILLNNAATTPPFETTLKAV
                     NNYLQTYGALHRGAGPHANITFQKAEEALAVLRKFLNVSDEQTLLFTQNTSAAINLFI
                     RLLRLKEDDVIITSIIEHTSNNLPWHFNTKAKPIFANAFDDGSLDYDDLEKKVKEHSN
                     KLKLIAITGASNLTGYIPDIPRIATLAHQHKALLFVDAAQLAPHRLIDMKKQGIDVLA
                     FSAHKVYAPFGLGVLALPKKILEQIPVDPGGGSIDMISESGEIVWAPPTIRHQTGTWN
                     VTGIIAAAASCKAILDTGWDKIIKHEKELVNYAVKQLQTIPNLTLYVPAEKYLTEDRI
                     GTFTFNLKGYHHALLSAILENEYGIETRAGTICNHRLVRRWFKIGDKTQKEIERKIAG
                     GDRLASYGIVRASLGIHNTKEDVDALVSALKEIAESGNKIKYKPVPVEEIYVCED"
     gene            complement(5272..5355)
                     /locus_tag="VI981_01445"
     tRNA            complement(5272..5355)
                     /locus_tag="VI981_01445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5319..5321),aa:Leu,seq:gag)
     gene            complement(5462..>5640)
                     /locus_tag="VI981_01450"
     CDS             complement(5462..>5640)
                     /locus_tag="VI981_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY4479006.1"
                     /translation="TVRSTVAGVGVEKVLPIYSPNITKVEIVSSPKKVHRSKLYFVRK
                     FSGAKTRQRLGVSV"
BASE COUNT         1536 a         1295 c         1290 g         1519 t
ORIGIN      
        1 atggttcgag tccatccacg cgcaccaaat tggacttcgt gattttctgg aagccaaagc
       61 gatagtttgc tcgtcccgcc tgctggcggg actcgcaagg aattgggcgg taaaatcaag
      121 cggcggggcg aaatcccaag acgcaatttt ttcggaaatg aggcggttcg ttccaatttt
      181 ttgcgcaaat tgagatatct ctcccagaga ttccgcagaa gcaatttttt ccgcttggca
      241 taaggttttt atgtaatctc gtgttggttc gatccaacca ctcatccact tccgcagaag
      301 caactccttt tcgtttttga gagatatttt ttgctcgaca agctcgcctt ttttcttgcg
      361 ataggtgtcc tcgtcgatca gattatccaa gtagccagtt acgagcttgt cgagcttctc
      421 atcgagcaaa aacattttct ttgagattgc ttctgcggaa gtggtagcag attgagcacc
      481 cttcttttct tcagtttcga gcattgcata caattcatgc gcccaatcgg ctggcaaagc
      541 gattgctcgc gatctttcaa tgattttccg tttcaaatcg ttttcctgca catacttctc
      601 gctacacgcg ccgtgcttcc ttgtgcaccg ataatatcgg taagtgccac cattgccatg
      661 agtccactgt gccgtaatca tcgagccgca agagcaatta aaaagtccga caaacggaaa
      721 atcatgtttt ttcttgctct tgcggggctt gcgcctgttt gccaaaactt cctgcacttt
      781 cgcaaacaat tgctttgaga tgagcgggcg gtatttgcct tcgtgcgttt cgccgttcca
      841 gcgcataatt ccgatgtagc gatcattggt caaaagattt tcaatagagt agttggagcg
      901 cgcttttccg ccgccaagct cggcgaggcg agccgaaatt gatttgagcg tgtgtctgac
      961 ggtcgcaaac tcttcgtaga tttttttgac gatttttgct tctttcggct ccggcacaat
     1021 gtttcttaat cggtggtcat agacatagcc gaaaggtttt tgcccgggcc attcgccacg
     1081 gcggattttt tggcggattc cacgcttcac attttcaaca aggttgtcag aaaaatattt
     1141 ggactgcccg aaagccactt gcagcataaa caagccttgc ggtgttggct caaaccaaaa
     1201 agtcgggaat tttagctcct tgataacgcc cgtatcaacg agataaataa tttgcccgcc
     1261 atcaacacta tttcttgcca atctatcaga ctctctatga taaactgaaa gtactcacgc
     1321 cgccggcttt tatggctcat ggcatcctgc ctccggagtt tatccggtct ggctttggtc
     1381 agaccgccta aaggcctgcg cgcggagctt tctgaacgcg cgacttaata gagagttgcc
     1441 ttggtccgcc ctaagcgggc ttatggcttg cgataaagga atgttcccgt ttactaaagc
     1501 ctcgttttgc agacgcgaga aattatcttg aaaggaggtg attaacaatg aatggaccga
     1561 tttatgaaca atcggtgatt gtcggcgtgg acgcgcataa gtattctcat acggccgcgg
     1621 ctgtggacgc ctgggggcaa gagagggggc ggctgaactt taccaacgac tctttagctg
     1681 actgcttgtc ctggctaaag actttaggcc ggcgcgaaga cgttattgtg gccgtggaag
     1741 atgccaacag ctacggctgc catttagttt ccgccttggc cgggcagaaa ttcgctatcc
     1801 gctctgtacc cgcggtttta actgaacggg ataggcaaca tagcacgcag cgcgataaaa
     1861 gcgatttttg gacatcccgc ttgccgaagt aagccgagaa gttcgcatcg gactgaggag
     1921 cggagagtat gccttgcaac ggtactatcc acgcccggtc ttctattttg tgatgctgaa
     1981 cttgttgtcg gagctcatcc aaggttcaga actacagagg gtgttcacga gtagcgacac
     2041 ttcattgaac ggaatacgat cctacgtgga catgagctca caacatccgt cgcacgcgca
     2101 gatgtttccc gggtactcgc cttcaaagac aagtgctttg attgcggaga ttctggcaac
     2161 atcgtaaaac tggccgtgtc ggttaacagt catcgggacg atacttgcgt gtcgtcccga
     2221 ttttattttt cgttctccgt aataccacca aactcattgg tggatttcat tcaacgctat
     2281 ttttccaatt ctacgaagta gacgacgcgc tgctctgtat ctctttgtga gtggtttgta
     2341 aaaatttcct tcatcacatt gaatcataca aaccacacac aaaaaatcgt tatcgagccg
     2401 aaccgcacaa aaatcatgga agatcgtttt atccgttgcg ctcttttgtg ccatgatacg
     2461 attgtgcgct acgttcttcg tgagccgctt accacgaaat gttgagagaa gcgcgtagac
     2521 atatttaccc aagctaagga aattgtgtct cgctactcct ttcaaacatc gcatctgagc
     2581 atcggaagcg aaaaacgtca tagtgtttaa gaacgacggc tggctgacag caaaaaattt
     2641 ccggcagagc ggagcgcagc cgagatgaat cgatttcgga aaaccattgc tgaggccttt
     2701 atagaaaagc aaaatatcat cggcagttgt atagttgtaa tcctttagac cacgggcttt
     2761 cgtcggcatc attggaacta tcatctttgt gttgctggcg ccgattttgt tgatgaattc
     2821 attcactcga tcaaccgtga gaagctcaat tagctgattc gtcgcgctgt tgtcggagta
     2881 agccagcata aagtaggcca actcttcgag cgtcagggcg ataggtgcgc ctacatactt
     2941 tagtaagcca gaaccaccga cgaaagtatg cacagtgagt ttttgtcgcg cgtcgtagtt
     3001 ttctgaagcg atttcctcgg ctaacgcgcc gagcacaaat agtttcatca agctggccgc
     3061 tggtcgcctt atgtcgcttt gccaggagaa aacttcttga cgatctgaaa taagagaagt
     3121 tctaaaacca atagaaaaac gatatcgcac ttcgtatgag tgaattaaat cttgaagggc
     3181 tgtgtttctt aaggacggcg tgatttttga agattctcca tacatatctt tattttgcat
     3241 cattttgtca accaatacaa gtatttttac gcttcacctc tacctgcctg ttagggcaga
     3301 gcgccgaaat atttttttgg ggcacgcgaa atccaaaaat tggggaaaaa aatttgcgac
     3361 catacaaata tctcttgccc ttgccccacc gctcccatgc gcgggcaaga ttatggaact
     3421 ccctctcaat ttgatttttc ttaaattttg aagtatcata aggtcaagtg agaagtgctg
     3481 atacaataat tctactactc tgatactata tgtcaaacat ttctaaaaat ataagaaaaa
     3541 tgcgagaaat gaaagggctt tcgcaagaga aactcgcaag attagctgat gttgctaata
     3601 atacgattgt taaaattgag gcaggaaaaa atcaaaaccc tacgcttgat acactcaata
     3661 aaattgcgaa agcccttgaa gtaacgattg atgatttagc gaataacaaa tatgccaaat
     3721 aatttaccaa aagtcgttgg aacagacgat ataataaaat tgagaaacgg aaaaaaagcg
     3781 cgtgtgattt tgctaaataa cgcggctaca actccgcctt ttgaaactac actcaaagcc
     3841 gtaaataatt atttgcaaac ttatggcgca ttgcaccgcg gagcggggcc gcacgcgaac
     3901 attacttttc aaaaggcgga ggaagcgtta gcagttttgc gtaaattttt gaatgtgagc
     3961 gatgaacaga cattactttt tacgcaaaat acaagtgcgg ctatcaatct ttttattcgc
     4021 ttgcttcgct taaaagaaga tgatgtcatc attacttcaa ttattgaaca cacctcaaat
     4081 aatttgccgt ggcacttcaa cacaaaagca aagccgattt ttgcaaatgc gtttgacgat
     4141 ggctcgcttg actatgatga cttggaaaag aaagtaaaag agcatagcaa caaactaaaa
     4201 cttatcgcta tcactggagc ttcaaatctc acaggctaca ttcccgatat tccaagaatt
     4261 gccacgctcg cacatcaaca taaggctttg ctttttgtag acgccgcgca actagcgcca
     4321 catcgcctaa ttgacatgaa aaagcaagga atagacgttc tcgcgttttc cgctcataaa
     4381 gtatatgcgc catttggttt gggcgtgctg gcgcttccaa aaaagattct agagcaaatt
     4441 ccagttgatc cgggcggcgg ctcaatagac atgattagcg agagcggaga aattgtctgg
     4501 gctccgccca caattcgcca ccaaactgga acttggaatg taacgggaat catcgccgcc
     4561 gccgcgagct gtaaagcaat tttggatacc ggctgggaca aaattattaa acacgaaaaa
     4621 gaacttgtta attacgcggt taaacaactc caaactattc caaaccttac actttatgtc
     4681 ccagccgaaa aatatcttac tgaagataga attggaacat ttactttcaa tttgaaaggt
     4741 tatcaccatg ctttgctttc tgcgatttta gaaaatgaat atggaatcga aactcgcgcg
     4801 ggaacaattt gtaatcatcg tcttgtccgc cgttggttta agataggcga caagacgcaa
     4861 aaagaaattg agagaaaaat cgctggtggc gatcggctcg cttcatacgg aattgtccgc
     4921 gcgagtttgg gtattcacaa cacaaaagaa gatgtggacg cacttgtttc ggctctgaaa
     4981 gaaatcgcag aaagcggaaa taaaataaag tacaaacctg ttccagttga agaaatttac
     5041 gtttgcgagg attagtgata gattgttatt gaaggtcgca aatttttttc gccgaaatgc
     5101 cggaagcgag ggcgcggcgg aaggggggtt caagggggga attccgccac gcccgagcgt
     5161 tccgccggag cgaagcggag gcggtcagtt cttctcaaaa aaggttcgcg cattttcaaa
     5221 caaagggcac attagatttt gttagtgcaa agcgccttac aaaatctaat gtgcgcggga
     5281 gaggactcga acctccacgt ccttgcggac accagcacct caagctggcc tggctaccga
     5341 ttacagcacc cgcgcgctac ttccagtgtc aagtatcaag ttttaagtgt caagatatac
     5401 ttgaaactca aaccccattc ttcagttttt cctggcgcct gacacatgat acctgacact
     5461 tttagaccga aacccccaat ctttgcctcg tcttcgcgcc ggagaatttg cggacgaaat
     5521 acaatttcga acgatgcact ttcttcggac tgctgacgat ttcaactttg gtaatgttcg
     5581 gagaataaat gggaagaact ttctcgacgc ccacgccggc gaccgtcgaa cggacggtaa
//
LOCUS       DATDSU010000043         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_208972, whole genome shotgun sequence.
ACCESSION   DATDSU010000043 DATDSU010000000
VERSION     DATDSU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208972"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /gene="lexA"
                     /locus_tag="VI981_01455"
     CDS             complement(<1..572)
                     /gene="lexA"
                     /locus_tag="VI981_01455"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY4479007.1"
                     /translation="MLTQKQRQILTYIEQQIAKKEVAPTHREIARRFRLSSVATVHEH
                     LAALQKKGFLKRQRGQARAIVTSNHSSELVPIPLLGVISAGHPIEALENKETILVSKT
                     QLPLFEKAYALKVAGDSMIDEGIEDGDIVVIRKQETANNGDRVVALLNNTDVTLKRFY
                     KNSREIRLQPANKKYDPIIVGPDTPISIQG"
     gene            886..1233
                     /locus_tag="VI981_01460"
     CDS             886..1233
                     /locus_tag="VI981_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018883742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEY4479008.1"
                     /translation="MSDIAKFQKMAAAFRDARDWKQFHNPKDMALSLVLEAAEVMEHF
                     QWKTPEEVRAYLKEHKGEVAEELADVLHWIVLMSHDMNIDLAKAFEAKMKKNEQRYPV
                     EKSKGKHTKYNKL"
     gene            1239..>2038
                     /locus_tag="VI981_01465"
     CDS             1239..>2038
                     /locus_tag="VI981_01465"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HEY4479009.1"
                     /translation="MTQIQTFPFEKDKFDQLRSYHFGLNWPVVYIQENGTEMYIGQTT
                     NVFSRSNQHYDNPDRARLKRIHVLTDEEFNMSAAYDFESLLIQYVSADGKYKLQNGNG
                     GLINHNYYEKEKYLAKLETVWPQLQDKGLVKQNLKDIKNSEFFKYSPYKALTEDQLAV
                     AAVLEKSIKKRQPVTHIVNGAPGTGKSILALYLLKHLKERRDMQDVSMALVVPMAGLR
                     STLQRVVRRVPGLGAEMIIGPSDIAKKEYDLLIVDETHRLSRRVNLAN"
BASE COUNT          594 a          414 c          517 g          513 t
ORIGIN      
        1 attccctgaa ttgaaatggg agtatccggc ccgacaataa tcggatcata tttcttattt
       61 gccggttgca aacgtatttc ccgtgaattt ttatagaatc tctttagagt aacgtcggta
      121 ttgttcagaa gtgctaccac ccgatcgcca ttattcgctg tttcctgctt gcgaatcacg
      181 acgatatcac cgtcttctat gccctcatca atcatgctgt cgccggccac cttgagcgcg
      241 tatgcctttt cgaaaagagg cagttgtgtt tttgatacga gtatagtctc cttattttct
      301 aaagcctcaa taggatgacc tgcggagatt actccgagta ggggaatagg cactaattcg
      361 gacgaatgat tactggttac aatcgcgcgg gcttgcccgc gctgtctttt taggaatccc
      421 ttcttttgca acgcggcaag gtgctcatga acggtggcaa cagaagagag cctgaatctg
      481 cgagcgatct cacggtgggt gggcgcaact tcttttttgg caatttgttg ctcaatgtaa
      541 gtcaatattt gcctttgctt ctgagtcagc atctgatgat tgtaccaata atacccgaac
      601 aaaacccgaa agttatccac aggtgcaact tatgcagacg caatcttttc ttatgctagt
      661 gttttgcaaa acactagcat aagaaaatac agaacaaggg ggtagacggg cagttattcc
      721 ttgcgttgcc gaacggctca gacggggtct gagccgttga cgagcttgga tttactgaat
      781 ggaatcgagg ggggtttggg ttatatgcta aattcggcga aggagtacaa tggacgataa
      841 ttcatactga gcccgtcgag cgtacgtttt tgagtagata gatttatgtc agatattgct
      901 aaatttcaga aaatggcggc agcgtttcgg gacgctcggg attggaaaca gttccacaat
      961 ccaaaagata tggcgctttc tttggttttg gaggcggcgg aagtgatgga acattttcaa
     1021 tggaaaacgc cggaggaagt gcgggcatac cttaaagaac ataagggtga agtggcggaa
     1081 gaacttgccg acgtcttaca ctggattgtg ttgatgagcc atgacatgaa tatagacctt
     1141 gcaaaagcgt ttgaagcgaa aatgaaaaag aatgaacaga gatacccggt agaaaaatca
     1201 aaaggcaaac acacaaaata caacaagctg tagtctatat gacccagata cagaccttcc
     1261 cctttgagaa ggataagttt gatcaactca gaagctacca ttttggattg aactggcccg
     1321 ttgtttacat tcaagaaaat gggacggaga tgtatatcgg tcagacaacg aacgtattca
     1381 gtcgcagcaa tcagcattat gacaatccag atagggcaag gctaaagcgc attcatgtac
     1441 tgaccgacga agagttcaac atgtcggccg cgtatgattt cgaatcgctt ctcatccagt
     1501 acgtttcagc ggacgggaaa tacaaattgc aaaatggaaa tggcggattg attaatcaca
     1561 attattacga gaaggagaaa tatctggcga agctcgaaac ggtatggcca caactgcaag
     1621 acaaggggct ggtaaaacag aatctaaagg atattaaaaa tagcgagttt tttaaatact
     1681 ctccgtataa ggcacttact gaagatcagt tagcggtagc cgcagtgttg gagaaaagta
     1741 ttaaaaagcg gcaacccgtg acgcacatcg ttaacggcgc gccggggacg ggaaaatcca
     1801 tcctcgcgct ctatcttctc aagcacttga aagagcgaag ggatatgcag gacgtaagca
     1861 tggcccttgt tgtgccgatg gcgggtttga gatcaacact acagcgagtg gtaaggcgcg
     1921 tacctgggct gggggcagaa atgattatag gaccgagtga tattgccaag aaagaatacg
     1981 acctgctaat tgtcgacgag acgcatcgtc taagccgcag ggtgaatttg gcaaacta
//
LOCUS       DATDSU010000044         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_704703, whole genome shotgun sequence.
ACCESSION   DATDSU010000044 DATDSU010000000
VERSION     DATDSU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704703"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1278
                     /locus_tag="VI981_01470"
     CDS             283..1278
                     /locus_tag="VI981_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY4479010.1"
                     /translation="MYDHYIALDWAQSNMAIARMTKESEKIQTKDVPSNIKELQFYLT
                     HLKGKKILTFEETTTSQWLYTELKDYVDEILVCDPYRNHLLSEGPKTDKLDAVKLVQL
                     LRGGLLKPVFHSADQFIEMRKLISGYQDVVKAGVRLKNQRASLFRAKGQNKREKSLND
                     PIENFVLEGVDEDIASYEKRKKHYEEEMGKISKKYLVIRNLDTVPGIGLVGALKIAAT
                     VVDPKRFQKKGRWLSYCGLIKHEKMSGGRSYGKKDSRYCRTLKSVFKTAALSVIGENV
                     TNDFKNYYEELLTKKGCAEHNARHAVSRRIAILTLGVFKSGKPFSLKKKAELIQK"
     gene            1991..>2172
                     /locus_tag="VI981_01475"
     CDS             1991..>2172
                     /locus_tag="VI981_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479011.1"
                     /translation="MQSIAWITIFCFTANSLFAQVPHLQFEGVTSRAASELPLPPYLG
                     LNPLNISTEHGKVAEF"
BASE COUNT          688 a          439 c          507 g          538 t
ORIGIN      
        1 agacaggctc ttaaggcttc tcgggaatat caggcactga caaggcggtc tttattttct
       61 gtttttaatt gttcaatgct cgaataaatg tcgtctccaa caacaagcca atcatgtaag
      121 cgttcagccc cctcagcccc ctcggtaaat tggaaaaggt ggtctcccca aggttcgaaa
      181 ccttgatgag cactggacac gaagtgttcg gctattacaa tgagacgcgt agcttgcgaa
      241 taaacgcaac tacatcattg taaaaaataa ggagaccaca caatgtacga ccattatata
      301 gcattagact gggcacaaag caatatggcg atagcaagaa tgacaaaaga atctgaaaaa
      361 atccagacaa aagatgttcc atcgaacatc aaggaattac agttttattt aacgcatctg
      421 aaaggaaaga agatactgac gtttgaagaa acaaccacct cgcaatggct gtatacggag
      481 ttaaaggatt atgtggatga aatattagtg tgtgatccgt accggaatca tctgttgagt
      541 gaagggccca agacagacaa gttagacgcg gtgaaattag tgcaactatt aagaggcggc
      601 cttttaaaac ccgtatttca cagcgcggat caatttattg agatgaggaa attaattagc
      661 ggctaccaag atgtggtcaa agctggagtg cgtttgaaga atcaacgcgc gtcattattt
      721 cgagccaagg gtcaaaacaa aagagaaaag agtttgaacg atccgatcga gaactttgtc
      781 cttgagggtg ttgacgaaga cattgcctct tacgagaaac gcaagaaaca ctatgaagaa
      841 gagatgggaa agatcagcaa aaaatattta gtgattcgaa atttagatac cgtgccgggc
      901 attggtttgg tgggtgccct taagatagct gcaaccgtcg tggatccaaa acgattccag
      961 aagaaaggcc ggtggttgag ttactgcggg ctaatcaaac atgagaaaat gagcggagga
     1021 agaagttatg ggaagaaaga ttctcggtac tgccggacat taaagagcgt cttcaaaacg
     1081 gcggctctga gtgtgattgg ggaaaacgtg acgaatgatt tcaaaaatta ttacgaggaa
     1141 ttgctcacga aaaaaggatg cgccgaacac aacgccagac atgcagtgag tcgtaggatt
     1201 gcgattttga cattaggtgt ttttaagagt gggaaaccat ttagtctaaa aaagaaagca
     1261 gagttgattc agaaatagcg cagaccttta ggacccgcat ttttctttct gacttcagaa
     1321 aatgcaaaaa ggtcttacgg gtgggcacag agagttcaac taaggcgata ttccaatccg
     1381 aaagggttcg agaaaacagc ccctcggtta acccgtgctc tattttcctg aaatttattg
     1441 tagccccgag ggatagatta acaatcctta ggaagagctc acatagacgc gtagggatac
     1501 tgccaataaa gaaaagaccg gatgatgcag ttatttggac tgaagaagaa atgaaaaaac
     1561 tgcgggagct aatcacgata gacggccccc tcaaagtggg agaccgtaag tcagagcaat
     1621 tttaagaaac aaaattgctg ataggagaac tttttccttg acttactttt ccatagacgt
     1681 cattgcgagg aggagcgaag cgacgacgaa gcaatcccaa cgcttcacca aaagcccgag
     1741 attgcttcgt caggcctatg gccttcctcg caatgacgga cgatgggggt accctgaacg
     1801 cttacagttc cagcgaataa attcgctact acgaacaaca ctcatgctct aaccttttga
     1861 attcacacga cttacgatga cgtcttgcca tttttcaact atgtgttata tttaagcttt
     1921 ataaatcctc tggcctggaa ggacttaacg tttcgttgga agtaaattct ttataaaatt
     1981 tcgtatcata ttgcaatcca tcgcctggat tacgattttt tgtttcacgg ccaattctct
     2041 cttcgcccag gttccccatc ttcaatttga aggggtaacg tcgagagccg cctcggaact
     2101 gcctctgcct ccttatctcg ggctgaatcc cttaaacatt tctacggaac acggcaaagt
     2161 agcggaattt tt
//
LOCUS       DATDSU010000045         7560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_209312, whole genome shotgun sequence.
ACCESSION   DATDSU010000045 DATDSU010000000
VERSION     DATDSU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209312"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..2087)
                     /locus_tag="VI981_01480"
     CDS             complement(309..2087)
                     /locus_tag="VI981_01480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY4479012.1"
                     /translation="MSNRREKMETEWNLKMLYNSPDDPQIEIDVRTVEDKCDEFASKY
                     RHTIAYTLEENKLAEALSAYEALAEFVYASKPLMYLYLMQTLDSANALLPARIAKITN
                     RLTKAHNETLFFTLHLGKIPSEFKSRFLKSPKLKRHHYFLGRLFKSSEHHLSELEERI
                     LNLKRMPANSMWVEGVERSVNKLLVLHRKKQIPLGEAGNLIADLPKKERRQLHGAILN
                     ELVKVSEFAENEMNAILTDKKINDELRKFNKSYSATVLDYENDERTVETLVSVVSDRM
                     KISHRFYKLKAEMMRERELTYADRAASIGKTAEKISFDKALQLTEKAFYKAHPLFAQI
                     LLAFARKGQMDVYPKKGKSGGAFCWSSLNVPTYVMLNFVHTFDSVMTLAHEMGHAVHG
                     ELSKSQPILYQGSSIATAEVASTFFEQVLFEDVFETLSAKDKIISLHNRIQDDVSTIF
                     RQIACFNFEVELHNEVRKKGSLDKDAMAALLTKHMKSYLGPAVRVDQKDGYAFVQWGH
                     IRRFFYVYSYAFGQITSKALFAEYRKDSSFINEVKKFLEAGSSAQPETIFASIGIDVT
                     KPDFFKIGLQNIEKDIERLEKLIAKK"
     gene            complement(2171..2782)
                     /locus_tag="VI981_01485"
     CDS             complement(2171..2782)
                     /locus_tag="VI981_01485"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY4479013.1"
                     /translation="MMTKLDEMKKIRDEVLALTSSPLYAERVKNKVLPVIGEGSHDSK
                     IMFVGEAPGRNEAKTGRPFCGAAGKILDELLLSADIARKEVYITNIVKDRPPFNRDPL
                     SEEIASYGPYLDRQIDIIKPDIIATLGRYSMGYIMRKFGKDAELEPISKAHGKSYDAQ
                     ASYGPIKIIALYHPAVAVYNSNSKGELKKDFGQLGILYKKYRA"
     gene            complement(2828..3292)
                     /locus_tag="VI981_01490"
     CDS             complement(2828..3292)
                     /locus_tag="VI981_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479014.1"
                     /translation="MLHHQCTLDEIAGIANVSSMNELRDIALSVLERLQPPVAQVCGP
                     ITTGGRGTVVENMRFFSFAVKQLHESGIVVFDQMIFQTGMLKLRKVGSSEYSQDILDV
                     FYRGLFSSGHIRRLYFLPDWRSSIGATWERVEATRHQIEVLDYPLHLISDFA"
     gene            complement(3339..3947)
                     /locus_tag="VI981_01495"
     CDS             complement(3339..3947)
                     /locus_tag="VI981_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479015.1"
                     /translation="MEREDLFSHLKFNTQVLTTKELEVAFRAVDRARFLEEQYLPEAY
                     EDYAVPIGFGKMLAKPTILAFMLELLAPAAGHRVMEIPSGSGYGLALLSHIVGEKGRV
                     YGIEKEAELRESSKMALEEYGFSNFEIISPSPKIGLPDEAQFDRILIMASLPEVHEDL
                     VSQLRDGGIMVLAFEDSIVQVKKIRKNKTEERFFQGFHLDSL"
     gene            complement(4149..5006)
                     /locus_tag="VI981_01500"
     CDS             complement(4149..5006)
                     /locus_tag="VI981_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY4479016.1"
                     /translation="MSKYYNPKRSAKYIFDPKSQDPYRLSRSKVDLFIQCPRCFYLDQ
                     RHGVGRPPGFPMTLNNAVDALLKKEFDIHRANGTPHPLMRKYKIDAVPFQHEKIDEWR
                     DALRRGVSFLHKPSNLILRGGIDDVWVSPKGELMIVDYKATSKDEEITLDDAWKIQYK
                     RQMEIYQWLFEKNGFRVSKTGYFVYVNGKTNRKAFDGKLEFDVTIIPYTGDSKWVEKA
                     LLDMKKCLLDDRIPKQSVDCDYCNYVRVLGDSLRENLATHSSRKVTKKEVHNKKPSDD
                     KEYAGGLFS"
     gene            complement(5317..5790)
                     /locus_tag="VI981_01505"
     CDS             complement(5317..5790)
                     /locus_tag="VI981_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479017.1"
                     /translation="MSKKLYLFIGILIVIGAFYLWPHNTSDVDNSADDEGASAHRVGS
                     NAILVNNQMPGKNVAIALVALLEDGWVVIHKDSNGKPGQIIGTKYFKAGESRGFLELA
                     APSTSGTYYAALYSDNGDGRFDVTEDAAIVSDGEPMMMIFQIGPEFTDEPLPIVI"
     gene            complement(5816..6655)
                     /locus_tag="VI981_01510"
     CDS             complement(5816..6655)
                     /locus_tag="VI981_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEY4479018.1"
                     /translation="MQVLTKQIEIIKADGTKEFFSRDKLDESLVRAGASSELREKIIT
                     HVESELASGMTTTEIYRHAFRLLAVSEKPLAMRYSLRRALVGFGPTGFPFEDYVGEIY
                     KALGFEVEVGKILQGHCVAHEVDLIAYNGEKLIMGEVKFHNELGLKTDLKVILYVKAR
                     VDDLKEMEFFYGNKYQKVTEGALITNAKFTDNAMKYGTCQGITMLGWNYPQKGNLQDV
                     IEAHGLHPLSCLSSLPLSHKRELYDRGVVLCKSLRDESLVSLLRLSDEQQRSVKEELA
                     LLC"
     gene            complement(6844..7344)
                     /locus_tag="VI981_01515"
     CDS             complement(6844..7344)
                     /locus_tag="VI981_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479019.1"
                     /translation="MDPDINVENNSGKKVIVIILCVIAVALIGWGFYYSNKVKNSEEV
                     VTQEPPKEDGSELAVTVNTKHQYKNGVHTLAGQMDMPTPCHLLESKATKDTVQTSKFA
                     IEFTSTEKGGGCIDVITPRPFKVSFEGPKDIELKATLNGKSIKLNLFEVKPNEDLDAF
                     EINVKG"
     gene            complement(7379..7555)
                     /locus_tag="VI981_01520"
     CDS             complement(7379..7555)
                     /locus_tag="VI981_01520"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEY4479020.1"
                     /translation="MYYARIRYRQTLEPCLVRKKGTGRVLLEFKSSQRAVTPGQFAVI
                     YDEDVCLGGGEIVM"
BASE COUNT         1998 a         1818 c         1561 g         2183 t
ORIGIN      
        1 taaaaaggag tataccctga agtacctgcc cgccacaacc agagcgaaat gcgcagtagc
       61 tttaatacaa gaagatgata ttactaatta atcacgcccc ctcccatcgg tgtgtcaggc
      121 gatggcaggc gggcctgccc gccacagcca gagcgaaatg cgcagtagct ttaatacaag
      181 aagatgatat taccaattaa ttacgccccc tcccatcggt gtgtcaggcg atggcaggcg
      241 ggcctgcccg ccacaaccag agcgaaatac agtgagaagt gatctcactt cagggtaata
      301 gtcccgtatt atttttttgc aatcagcttt tcgagccgtt cgatatcttt ttctatattt
      361 tgtaaaccga tcttaaaaaa atccggtttt gtaacgtcga ttcctattga ggcgaagata
      421 gtctcgggct gggcactcga accggcttca agaaattttt tcacttcatt gataaaactg
      481 ctgtcctttc tgtattccgc aaagagagct ttgcttgtga tctgtccaaa cgcatatgaa
      541 tagacataaa agaatcgccg aatgtggccc cattgaacaa aggcatatcc gtctttctgg
      601 tctacgcgca cggcagggcc aagataactt ttcatatgct tggtgagcaa tgccgccata
      661 gcgtccttat ccagactgcc cttcttccgt acttcattgt gaagctcgac ctcaaaattg
      721 aaacaggcga tctgacgaaa aattgtactg acatcatctt gaattctgtt gtgaagagaa
      781 attattttgt cctttgcgga gagcgtttcg aaaacatctt caaataaaac ctgctcgaaa
      841 aaagtgctcg ccacttcagc cgtcgctatc gaagaacctt gataaagaat cggttgtgat
      901 ttggagagtt caccatgaac cgcatgaccc atctcatgag caagggtcat gaccgaatca
      961 aatgtatgca cgaaattgag catgacgtat gttggaacat tgagactgct ccaacaaaag
     1021 gcccctccag attttccttt ttttgggtac acgtccattt gcccctttcg agcgaaggcg
     1081 aggaggattt gggcaaacaa aggatgtgcc ttatagaagg ccttttcagt cagctgaagt
     1141 gccttatcaa atgaaatttt ctccgccgtc ttcccgatag aggcggcacg gtcggcatat
     1201 gtgagctcac gttctcgcat catttctgcc ttcagtttat aaaaccggtg acttattttc
     1261 atccgatccg aaacgacact gacgagcgtt tcgactgttc tctcgtcgtt ttcgtaatca
     1321 agcacagtgg cactgtaaga cttgttaaac ttccgaagtt cgtcgtttat tttcttgtcg
     1381 gttaaaattg cattcatttc gttttcggca aattcactta ccttcacgag ctcattcaaa
     1441 atagccccgt gaagttgtct tcgttctttc tttggcaaat cagcgatcaa atttccggct
     1501 tcaccgagcg gaatctgttt tttcctgtga agtacgagga gcttgtttac agagcgctcg
     1561 acaccttcga cccacatgga atttgcgggc atccgtttga ggttaagaat tcgctcctca
     1621 agttcggaca gatgatgttc acttgattta aacaggcgac cgagaaaata gtggtgtcgt
     1681 tttaatttcg gcgattttag gaacctactt ttaaattccg acggaatttt ccccaaatga
     1741 agcgtgaaga aaagtgtctc attgtgagcc ttggtgagcc gattcgtaat cttggcgata
     1801 cgcgcaggca aaagtgcatt ggcgctgtcg agcgtctgca tcaaataaag atacataaga
     1861 ggcttcgatg catagacgaa ttcagcaagc gcttcgtacg cactgagagc ttccgctaat
     1921 ttattttctt cgagtgtata ggcaatagtg tgccgatatt ttgacgcaaa ttcgtcgcat
     1981 ttatcttcaa ctgtccggac gtcgatctct atctgcggat cgtctgggga gttgtagagc
     2041 atctttagat tccactctgt ttccattttc tctctgcgat tagacatgac tcaatactaa
     2101 actcttcctt aaacctgcgc aaatttaccc cgttagaagc aggacgcccc gtgtgagctt
     2161 ctaacggggt ttacgcacga tattttttat acaggattcc caattgtcca aaatcctttt
     2221 ttagttcgcc ttttgaattt gaattgtaca cggccactgc aggatgatag agcgctatta
     2281 ttttgatggg tccatacgat gcctgcgcat cgtaagattt accgtgtgct ttgctaatcg
     2341 gctcaagttc tgcatctttt ccgaatttcc tcattatgta acccatggaa tatctgccga
     2401 gcgttgcgat gatatcgggt ttaatgatat cgatttggcg atcaagatat gggccgtagc
     2461 tcgcgatttc ttcggatagc ggatcgcgat tgaacggcgg ccggtctttg acgatattgg
     2521 tgatatagac ttctttacgc gcaatgtcgg ccgagagcaa caactcgtcc aagatttttc
     2581 cagccgcgcc gcagaatggc ctccctgttt tcgcttcatt ccttcccggc gcttccccca
     2641 cgaacattat ttttgaatcg tgacttccct ctccgatcac gggaagaact ttatttttta
     2701 cccgctcggc atacaaaggc gacgaagtaa gcgcgagcac ttcgtcgcga atttttttca
     2761 tctcgtctag ttttgtcatc ataaaaatat accattgcat caggtaaaca aaaaatgacc
     2821 caatgggtca tgcaaaatca gaaatcaaat ggagcgggta atcgaggact tcgatctggt
     2881 gccgtgtcgc ctcgaccctc tcccatgtag ctccgattga acttcgccaa tcgggaagaa
     2941 aataaaggcg tcgaatatgc cctgaggaga ataatcctcg gtagaagacg tcaagaatgt
     3001 cttgagaata ttctgacgac cctacttttc taagtttcaa cataccagtc tgaaaaatca
     3061 tctgatcaaa caccacgatt ccactttcgt gaagctgctt aaccgcgaaa gaaaaaaatc
     3121 tcatgttttc aaccacagta cccctgcctc ccgtggttat cggaccgcac acttgcgcaa
     3181 ctggcggttg caagcgctca agaacgctaa gggcaatatc gcgaagttca ttcatcgatg
     3241 aaacgttcgc aatccccgcg atttcgtcga gtgtacactg atgatgtaac atatctgccc
     3301 aagatactac tgaactgcga aagatagtca agatcggtct acagtgaatc aagatgaaac
     3361 ccctgaaaaa atcgctcttc tgttttattt tttcggattt ttttgacctg aacaatcgag
     3421 tcttcgaatg caagcaccat gataccgcca tcgcgaagct gggaaacaag atcttcgtgc
     3481 acttcaggca aggacgccat tattaagatt ctatcaaatt gagcttcatc cggaagcccg
     3541 attttcggcg agggacttat gatttcaaaa ttactgaatc catattcttc gagggccatt
     3601 ttgctgctct ctcgcaattc agcctccttt tcgataccgt atactcgccc cttttcaccg
     3661 acgatgtgag aaagcagagc aagtccgtaa cccgatcctg aaggaatctc catcaccctg
     3721 tgacctgccg ctggagccaa aagttcgagc atgaaggcaa gaatagtagg tttcgccagc
     3781 atttttccaa atccgattgg aactgcataa tcctcatatg cttcgggaag atattgctcc
     3841 tcaagaaacc gcgctcggtc tacagcgcga aaagcaactt cgagttcttt tgtggtcaga
     3901 acttgagtgt tgaacttcaa gtgactaaac agatcttctc gttccatctg ctgaatatac
     3961 cattccgaaa aaatcaaatc aaaaaagtta aaaaaagtca ctatactaac ttcgtgctat
     4021 agcacgaagt tagtatagtc ggagtttata cctgtatacc tatatatcgg tatatcagtg
     4081 tacctgtgca cttcggtata tcggtgtatc tgtgcaccat aggctatata tatcttacgt
     4141 ctgcaactct aggaaaaaag gcccccggca tattccttgt cgtcagacgg ttttttattg
     4201 tgaacttcct ttttcgtaac tttgcgagat gaatgagtag caagattttc gcggagagaa
     4261 tctccgagca cccgcacata attgcaataa tcgcagtcaa ctgattgttt tggaatcctg
     4321 tcatcaagaa gacatttttt catatcaagc agtgcctttt caacccattt tgagtcaccg
     4381 gtgtatggaa taatggtcac gtcaaactca agttttccgt caaaagcttt cctgtttgtt
     4441 ttcccattca catacacaaa ataaccggtc tttgaaaccc taaaaccgtt tttttcaaag
     4501 agccactggt aaatttccat ttggcgtttg tattgaattt tccacgcgtc gtcaagagta
     4561 atctcctcat ccttgctcgt tgccttataa tcaacaatca tgagctcacc cttaggcgaa
     4621 acccaaacgt cgtctatacc tcccctcaaa ataagattgg aaggtttatg caaaaagctg
     4681 accccgcggc gaagcgcgtc acgccattcg tctatcttct catgttgaaa aggaacagca
     4741 tcgatcttgt acttcctcat cagcggatgc ggcgtcccat ttgcacgatg aatatcaaat
     4801 tcctttttca aaagtgcgtc gacagcatta tttagtgtca tcggaaaacc ggggggacgt
     4861 cccacaccat ggcgttggtc aagataaaaa caacgaggac actgtatgaa taggtctact
     4921 tttgaacggg atagtctgta tgggtcctgc gattttgggt caaaaatata ctttgccgat
     4981 cgtttcggat tgtaatactt agacatttta aaagtataac tcgctcggtc aacttgtaca
     5041 aaagatgcac ggatattttc ctgctggaaa attcctcgtg ctcgctccga cgagcctcgc
     5101 aaggcacttt tgtacaatag acctcgctcc gacggtcaag atcgagccga cgcgcagttc
     5161 aaggctcttg aacacggata ttcgctccgc cgcggtaaga aaaccttccg taaggcatta
     5221 caaatgagca tggctcactc taacgtaaca caatgaacgt tttaaacaca gatatccgct
     5281 ctgcgtcaac atgaaaaagt cgcgcgtcta aaaaaattaa atgacaatag gaagtggttc
     5341 atcggtaaat tctggaccaa tttggaaaat catcatcatc ggctcgccgt cgctcacgat
     5401 tgcggcgtct tcggtcacat caaaacgacc gtctccatta tcactgtaga gagccgcgta
     5461 gtacgttccc gaggtactcg gtgccgcaag ttctaaaaag ccgcgagatt ctccggcttt
     5521 gaaatacttt gttcctatta tttgtcccgg cttgccgttt gaatctttgt gaatcacgac
     5581 ccacccgtct tcgagaagcg ctacaagcgc aatggcaaca tttttcccag gcatttggtt
     5641 attcaccagt atagcgttcg aaccgactcg gtgcgcgctc gcgccctcgt catccgcact
     5701 attatccaca tcggatgtgt tatgcggcca aagataaaat gcgccgatta caattagtat
     5761 gccaataaaa agataaagtt tttttgacat ttgaatgcaa atacgtaccg tgctcctaac
     5821 agagcagtgc cagttcttcc ttcacacttc tctgctgttc atcagataga cgcagtaacg
     5881 agacaagact ttcatcacga agcgatttac acaatacaac gccacgatcg tataactctc
     5941 ttttatgaga cagtggaagc gaagaaaggc atgacagagg atgcaaccca tgcgcctcta
     6001 tcacatcctg cagatttccc ttttgggggt aattccagcc aagcatcgta ataccctggc
     6061 acgtgccgta tttcatggca ttgtcagtaa actttgcatt ggttattaac gcaccttctg
     6121 taactttttg atatttattt ccgtaaaaaa attccatttc ttttagatca tcgactcgcg
     6181 ctttaacgta aagaattact ttaagatcag ttttcaaccc aagttcgttg tgaaatttga
     6241 cttctcccat gataagtttc tcgccattat aagcaatcaa gtccacttca tgggcaacac
     6301 agtgtccctg aagaattttt cccacttcaa cctcgaagcc gagcgctttg taaatttctc
     6361 caacataatc ttcaaacgga aagccagtcg gcccaaaacc gacaagtgcg cgtcgcaaag
     6421 aataacgcat tgcaagcggt ttttccgata cagctaaaag tctaaacgca tgtctatata
     6481 tttccgtcgt ggtcatgccg ctcgcaagct cgctttcgac gtgtgttatg attttctccc
     6541 gaagctcgct cgacgcaccc gctcggacga gcgattcgtc gagtttatcg cggctgaaaa
     6601 attccttagt gccgtcggct ttaatgattt caatttgttt tgtcagaact tgcattttat
     6661 attctatcta agcgagtgca ttcctcttta atcttccgac taagcgagtg ctcatctttt
     6721 ttgatctttc aactaagcga gtacattgct ttttaaatgc cttggaaggc gcgacggcgc
     6781 cggacagaga aaatctttag caaaagattt ttgtgtattt aaaaaggaat gtgcgagcgc
     6841 catctaaccc ttgacgttta tttcaaacgc gtcaaggtct tcattcggtt ttacttcaaa
     6901 aagattcagc tttatgcttt ttccatttaa tgtcgccttc agctctatgt ccttgggacc
     6961 ctcgaaactg accttgaacg gtctaggtgt aatcacgtca atgcatccac cccccttctc
     7021 tgtgctagta aattcaatcg caaacttact agtctgaaca gtgtcttttg tggccttgct
     7081 ttcgagaagg tggcaaggag tcggcatgtc catctgtccg gctaaggtgt gtaccccatt
     7141 cttatactga tgttttgtgt tgacggttac cgctaactcg cttccgtctt ctttcggtgg
     7201 ctcctgggtc actacttctt cagagttttt aactttattc gaataataga acccccaccc
     7261 aatgagcgcg acggcaatta cacataagat gatcacaatg acttttttac cgctgttgtt
     7321 ttccacgttt atatctggat ccatgatgta agtgttaact tctagattga taattatatc
     7381 acatcactat ttcccctcct ccaagacaga catcctcgtc atatataacc gcaaattgcc
     7441 cgggagttac agccctttga gacgacttga attccaaaag cacgcggcct gtaccttttt
     7501 ttcggacgag gcaaggttcg agtgtttgtc gatatcggat acgggcgtag tacactttat
//
LOCUS       DATDSU010000046         4098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_878639, whole genome shotgun sequence.
ACCESSION   DATDSU010000046 DATDSU010000000
VERSION     DATDSU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878639"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VI981_01525"
     CDS             <1..269
                     /locus_tag="VI981_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479021.1"
                     /translation="RPGFVSYVFSPHLFLAAAIVFGALWGWKGDGGWKLEAGIRGKVV
                     ALIVGLLLAWVVWTMGRSLEEFRILVTLAAFLTPFTIYRLLSTK"
     gene            273..677
                     /locus_tag="VI981_01530"
     CDS             273..677
                     /locus_tag="VI981_01530"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY4479022.1"
                     /translation="MSDCLFCKIIAGDIPCHKIYEDDRTVAFLDIFPVSEGHALVLPK
                     VHAVHLLETSSEDALAMMATVKKITPAILQAVGGDGFNLGGNNGESASQEIPHTHLHI
                     MPRKTGVPRSFEKTQGDQAGLAILAEKIRQTL"
     gene            674..1492
                     /locus_tag="VI981_01535"
     CDS             674..1492
                     /locus_tag="VI981_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479023.1"
                     /translation="MIIEELIEIARRGAEEPLDFWLDHYQRAAPQIISDLSAVAATLE
                     SDTVIAAQLQSCINLFKTSHPDEFELAKSLSSIATVFAQSSPDQGDGAVGKFLGSVRL
                     FYGRAQTAALFTNGREQLKATLPLDQQQAFDLRLFQNEGMTYCLAFHLALYKAIQDVP
                     DEEQKRQLIDGKEINLGFGGLPGLKLDFEQDEVLTKFILKILNDDLRSGLLKAYYEAK
                     VSIVEQVSVTEVIHAFRQWLTALIAAFQKIGIDRFISDFFKPYGDNPRFVEISL"
     gene            1498..3894
                     /locus_tag="VI981_01540"
     CDS             1498..3894
                     /locus_tag="VI981_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479024.1"
                     /translation="MGNNKVEETKPVENEDGAEKGVHATYDTKEAKAADQLRSALLKK
                     KARGKKGSQPIDASLDVPEPVTLNTIRKAEDDRRAEEHRLKQLEPLLNSLSKYESQPS
                     GPLYSRDFEFNILRAYKDQIKSDPELTQRVFNLIPKMDDYNASQSLVYFLEAGLTCPE
                     AQVGMVKNFVERNIDKHIHVSDVEKIEKLVGFQGDFTPEQRNRMFASRFTVDHGFWGK
                     RSARAQLDNRSSGYTLQFSDSHVPMFFPADRQIIEEVFEKLKGEFPQLQRNPLTFGAD
                     VRNYVEQNILPDVLNGDERIVELMLNARNLEEIKKVAKMSPERLTLMNSTRAGQERMR
                     LVVEYRRAVARIDQDEESALIDLRSKFQEGALTSEEKRTAESRMKADFDLRRVEIEEE
                     FNVNLRKTFEERDENVRGMVEGGLLEINKKWSGELRPQVKAGQQDAVKDRAFTLAIEK
                     QNQAFFAVKEGETPDEAHERILGEIKKRIEDRFVGLQASRDKGSLEKSEEQQGRIGLG
                     RLFEGLFQGENKQYLQEILSDKEYVKSLLFQYGDKKRKGGLPVPIESFQMDEHQWRPD
                     ALKELIDLHEKNFPGETHAMVARVVDGINQEVEAELFQIRKRNKIETGWPSGDEDDLN
                     FGFPNVETGRYDRWLNFSQKFEIHVPFDQLPGVTPEEIAKINPSHELNRPDEVFSEEY
                     LPLFQKTFGVERTKRLLMEILMPELIEDKNRRKENEHTQYSGGGEYLYSPKRIFRFAK
                     SVGIDLRGALAMVEEMEERARVAKEEGQFTRRDPSTLQSGMFEAYHGELRAFLKASGM
                     "
BASE COUNT         1196 a          797 c         1063 g         1042 t
ORIGIN      
        1 tgcggcctgg gtttgtgagc tatgtttttt cgccgcatct cttccttgcg gccgcgattg
       61 tatttggagc actgtggggt tggaaagggg atggaggctg gaagttggag gctggaatac
      121 gagggaaagt cgttgcactc attgtgggtc tcttactcgc ctgggttgtt tggacgatgg
      181 gacggtctct tgaagagttt cgaattctcg tcaccctcgc cgcgttcctc actccattta
      241 ctatctaccg tctactatct acaaaataat gtatgtccga ctgtttgttc tgcaaaatta
      301 tcgctggcga cattccgtgt cataaaattt acgaagacga tcgcacggtg gcgttcctgg
      361 atatttttcc tgtgagcgag ggtcatgcgc ttgtgttacc aaaagtacat gcggtgcatt
      421 tactcgagac ctcgagcgag gacgctcttg ccatgatggc aacggtaaaa aaaatcacgc
      481 ccgcgatttt gcaggccgtc ggtggtgatg gattcaatct tggcggcaac aacggcgagt
      541 cagcgagtca ggaaattccg cacacgcatt tgcacatcat gccgcgcaaa accggcgtgc
      601 caaggtcatt cgaaaaaacg caaggtgatc aagccgggct cgccatcctt gcggaaaaga
      661 ttcgacagac attgtgatta ttgaagaact tattgagata gcgagacgtg gggcggaaga
      721 gcccctggat ttttggctgg atcattatca acgagctgcc ccacaaatca tttcggatct
      781 aagtgccgtt gccgcaacac ttgaatcgga tacggtcatt gctgcgcagc tccaatcctg
      841 cattaatctt tttaaaacat cgcatccgga tgagttcgaa ctagccaaat ctttgtcttc
      901 cattgccacg gtttttgcac aaagcagtcc ggaccaaggc gacggagcgg tggggaagtt
      961 tttagggtca gtcagactgt tttatggtcg ggcgcaaaca gccgcccttt ttacgaatgg
     1021 acgagaacag ctcaaagcaa ctcttccgct cgatcaacaa caagcatttg atttgcgctt
     1081 atttcaaaac gaaggcatga catattgtct ggcatttcac cttgccttgt ataaggcaat
     1141 ccaagacgtg ccagatgagg aacaaaagcg ccaactgatc gatggaaaag agatcaatct
     1201 tggctttggt gggcttccgg gactaaaatt agattttgag caagatgaag tgctgactaa
     1261 attcattcta aaaattctta atgatgattt gcgcagcggg ctccttaaag catactatga
     1321 ggccaaggta tcaattgttg agcaggtttc ggtgacagaa gtcatacatg catttcgtca
     1381 atggctcacg gcactcatag ccgcctttca gaaaataggc atcgatcgtt ttatcagcga
     1441 tttttttaag ccttacggcg ataatccaag gttcgtagaa attagtttgt aaaaagtatg
     1501 ggaaataata aagttgaaga aacgaagccg gttgaaaatg aagacggcgc tgaaaaaggt
     1561 gttcatgcaa cctacgatac aaaagaagct aaggcagccg atcaattacg ctctgctttg
     1621 ctcaaaaaga aggcaagagg aaaaaagggg agtcaaccca tcgatgcttc cttagacgtc
     1681 ccagaaccag tgacgcttaa tactattcgt aaggccgaag atgataggcg agcagaggag
     1741 caccggctca aacaacttga accattactc aattctcttt ccaaatacga aagtcagccg
     1801 tcggggcctt tgtactctcg tgatttcgag ttcaacatcc ttagggcgta taaggatcaa
     1861 attaaatctg atcctgagct gacgcaacgt gtttttaacc ttattccaaa gatggacgat
     1921 tataatgcgt ctcaatcctt ggtgtatttt cttgaagccg gtttgacatg tcctgaagcc
     1981 caggtcggca tggtgaagaa ttttgttgaa cgtaatatcg ataagcatat tcacgttagc
     2041 gatgtcgaaa aaatagaaaa gttggtaggt tttcagggtg attttactcc tgagcagcgg
     2101 aacagaatgt tcgcgtcacg atttactgta gatcatggtt tttggggaaa aaggtccgcg
     2161 cgggcgcaat tggacaaccg aagcagtggg tatacactgc aattttctga ttctcacgtc
     2221 cctatgtttt ttccagccga tcgacaaata atagaggagg tgtttgaaaa attaaaaggg
     2281 gaatttccac agttgcagag aaacccatta acctttggag cagacgtaag gaattacgtt
     2341 gagcaaaaca ttttgcccga tgttttaaac ggagatgaga ggattgtaga attgatgctt
     2401 aatgctcgta atcttgaaga gataaaaaag gtcgcaaaaa tgtctcctga gcggctgact
     2461 cttatgaata gtacgagagc ggggcaggag aggatgaggc ttgtcgtcga gtatcgtcgc
     2521 gccgtagctc gcattgatca agacgaagag agtgcattaa tagatttacg tagtaaattc
     2581 caagaaggag cgttgacgag cgaggaaaaa cgaactgcag aaagtcggat gaaagcggat
     2641 tttgacctgc ggcgtgtcga aatcgaagaa gaatttaatg taaatctacg taagacattt
     2701 gaggaacgcg acgaaaatgt gaggggaatg gtagaggggg gcttattaga aatcaataaa
     2761 aaatggtctg gtgaactacg tccacaagtt aaggcgggtc aacaagacgc cgtaaaggat
     2821 cgtgcattta ctttggcaat agaaaaacag aatcaagcat tttttgccgt taaggaagga
     2881 gaaacacctg atgaggccca tgaacgtatt cttggcgaaa tcaaaaagcg aattgaagac
     2941 cgttttgttg gtttgcaagc ctcacgcgac aaaggtagtc tggaaaaaag tgaggagcaa
     3001 cagggacgca tcggattggg tcggttattc gaaggtttgt ttcaaggcga aaataagcag
     3061 tatcttcagg aaattctgtc agataaggag tacgtgaaga gtttgctgtt tcaatacggc
     3121 gacaagaaaa gaaagggtgg attacctgtt ccaatcgaat catttcaaat ggacgagcac
     3181 cagtggcgtc cggacgcact caaggaattg atcgacttac atgaaaagaa ttttcctggt
     3241 gagacccatg ccatggttgc tcgtgttgtg gacggtataa accaggaagt cgaagcagaa
     3301 ttatttcaga ttcgtaagcg gaataaaatt gagactggat ggccctcggg ggacgaagat
     3361 gatctaaatt tcggttttcc gaacgtagaa accggacgat atgatcgttg gttaaatttc
     3421 tcacaaaaat tcgaaataca tgttccattt gaccaattgc ctggcgtaac gccggaagag
     3481 atcgcaaaaa ttaatccgtc tcatgaattg aacaggcccg atgaagtatt ttctgaagaa
     3541 tatttgccac tctttcaaaa aacgtttggt gtggagcgca caaaacgttt attaatggaa
     3601 atactcatgc cggaattgat tgaagataaa aatcgtcgca aagagaatga acacacacaa
     3661 tattccggcg gaggtgaata tttgtatagc cctaaacgca tttttcgttt cgcaaaaagt
     3721 gtaggaattg atctgcgggg cgctttagct atggtagaag agatggagga acgcgcgcgt
     3781 gtggctaaag aagaaggaca gtttacgcgt agggatccct ccactctgca gtcgggtatg
     3841 ttcgaggcat atcatgggga actcagagct ttcttgaagg cttcgggtat gtagatgaaa
     3901 gctttggtga cgaagaaaaa ttagcttatg tccaattgga aaaaactcag cgagcggatt
     3961 gtctacgaaa atccttggac aaaagtgcga gaggacgagg tgattcagcc cgggggcgct
     4021 gagggagggt acggagtcgt cttgcgaaag cttggcgtgg gaattttggc agtcaacgac
     4081 aagaatcaaa ttttcgtt
//
LOCUS       DATDSU010000047         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1218687, whole genome shotgun sequence.
ACCESSION   DATDSU010000047 DATDSU010000000
VERSION     DATDSU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218687"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VI981_01545"
     CDS             <1..997
                     /locus_tag="VI981_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479025.1"
                     /translation="FEECERQKIDLSKYEFDVVSGGEGFAENWRDTLIKKIGPSRSIF
                     SVYGSTDKGLGEGLETNFAYAVRSLIHVAVTLMTNETAAQKIMMLRFGISELPFSKET
                     AKNFLLDFLKREENIFRIPMVFQFDPTQYYNENITVLDPKQDRELTEFVTTVLTPNVT
                     IPRIRYNVHDEGFIMTYEEITRILKKYAIELESFNPRKDAYLDLHLPFLFIYGRSDGT
                     ISVDGANIFPQDIEEILRKDEELSTVVNSFQLFVTENYHVGIAIELASGNVPNEQIGE
                     KIKNYLTACLAETSFGYRELVHDQLPSADVKIELYALNDGPYFKEKTLKMRYVKH"
     gene            984..2429
                     /locus_tag="VI981_01550"
     CDS             984..2429
                     /locus_tag="VI981_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479026.1"
                     /translation="MLNIDLYPLLTQLSASLGQRYETWRRKKIISFLSRVNPERLEHW
                     GEKAIVRAFRNAANRVPYYRALLRQVGINPSSIKSIKEWGELPLLDKSIFQKAEIADL
                     TIGGSLSNIKGILTSSGFSGKFSFGVNSKKNWQNTARMIDLVLDYALKIGSRKTLAIN
                     CLPMGVKMFTNEMVLAEVSVREDMALALIENFHKFFEQIIIVGEAFFLKSLVEEGVRS
                     GLDWKSLNVSLIVGEDSFPESYRTYMQALLGISHDTYPMRRLIGSSLGVAEVDLCLFH
                     ETADTIRIRRFADTNLDFRRTLFGDELTAVPMLFQFYPQRTFIERDLGNHFSPTILTT
                     TTFSPGDIPLIRYQTGDTSLVIPYKKTEKILRSFGKEDLVPRLKLPLIAVKSRHASTP
                     GLTALADEVRDRIFRDPEIARRVTGYFKIRDNAEEKRIDLQLTKDVSTPENVAILKKR
                     FIITGISINPIAYYDFHEALILNYNKKFHHT"
     gene            2430..>2701
                     /locus_tag="VI981_01555"
     CDS             2430..>2701
                     /locus_tag="VI981_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY4479027.1"
                     /translation="MSKLHSSLGKIGFVLHGGFMRGAFQVGALKAFDEFRLYPSYIVG
                     ESVGAINGAAFAAGKVELLWKIYLEIAKNPHRYIYRYNFYMLLRAF"
BASE COUNT          776 a          596 c          568 g          761 t
ORIGIN      
        1 ttttgaggag tgcgagcggc aaaaaatcga tctctcaaaa tatgaattcg acgttgtctc
       61 cggaggagag ggttttgcgg aaaactggcg tgatacgctt attaagaaaa ttggaccgag
      121 ccggagtatt ttttcagtgt atggttcaac cgataagggg ttaggcgagg gccttgagac
      181 aaattttgct tatgcggtac gctcgcttat tcacgtcgca gtcacactca tgaccaatga
      241 aacagccgcg cagaaaatca tgatgctccg ctttggaatt tcagagttac cattttcaaa
      301 ggaaacagcg aagaactttc ttctcgactt tttaaaacgt gaagaaaata tttttagaat
      361 tccaatggta tttcagtttg acccaacgca atattacaac gaaaatatta cagtgcttga
      421 tcccaaacaa gaccgtgaac taaccgagtt tgttaccaca gttctcaccc cgaatgttac
      481 cattcccaga atacgctaca acgttcacga tgagggcttc attatgactt atgaggaaat
      541 cacccgtatt cttaaaaaat atgccatcga gctcgagagt tttaacccgc gaaaagatgc
      601 gtatcttgat ctccatctgc cgtttttgtt catatacggc agatctgatg gcacaatttc
      661 cgttgacgga gccaatattt ttccgcaaga cattgaagag atattgcgaa aagacgagga
      721 gttaagcacg gtagttaaca gctttcaact ttttgtaacg gaaaattacc atgtgggcat
      781 cgcgattgag cttgcttcag gcaacgtacc taatgaacag ataggagaaa aaattaaaaa
      841 ctacctaaca gcgtgcctag ctgaaactag ttttggctac cgcgagcttg ttcatgacca
      901 gttgccttct gcggatgtga aaatcgaact gtatgcctta aatgacggcc cctattttaa
      961 agaaaaaacc ctcaagatgc gttatgttaa acattgatct atatccgctt ttgacacagc
     1021 tgtctgcttc tctcggtcaa cgctatgaaa catggcgaag aaaaaagatt atttcgtttc
     1081 tttcacgtgt aaatcccgag cgtcttgagc attggggtga aaaagcgatc gtgcgcgcat
     1141 ttcgtaacgc agccaatcga gtcccttatt atcgagcctt gttaagacaa gtgggcatta
     1201 atccatcctc aatcaaatcc ataaaagaat ggggcgagct gccactctta gataaatcca
     1261 ttttccaaaa agccgaaatt gctgatctta ctatcggcgg tagtttaagt aacatcaagg
     1321 gcatattgac gagttcagga ttttccggaa aattctcttt cggtgttaac agcaaaaaaa
     1381 actggcaaaa tacagcgcgg atgattgact tagtgcttga ttatgcgctt aagatcggta
     1441 gcagaaaaac actagcgatc aattgtctgc ctatgggggt taaaatgttt accaacgaaa
     1501 tggtgctagc ggaagtgagc gttcgggaag atatggcact tgcactcatc gaaaactttc
     1561 ataagttttt tgaacaaatt attattgtcg gcgaagcatt ttttttaaaa tcgcttgtgg
     1621 aggagggtgt gcgtagcggc ctggactgga aatcgcttaa tgtcagtctt attgtcggag
     1681 aagacagctt tcccgagtcg taccgaacct atatgcaagc gcttcttggg atttctcatg
     1741 atacctatcc gatgcgtcgc ctcattggct cttctcttgg agtcgccgaa gttgaccttt
     1801 gtctctttca cgagactgct gacaccattc gcattcgtcg ttttgctgac acaaacctag
     1861 atttcagaag gaccctcttt ggtgatgagc ttacggctgt gccaatgctt ttccaatttt
     1921 acccgcagcg tacctttatt gaacgtgacc tcggtaacca tttttctcct actattctta
     1981 ccaccacaac cttttcccca ggcgatatcc ccctcatccg atatcagact ggcgatacaa
     2041 gtctcgtaat accttacaaa aaaacagaaa aaatcttacg ctcatttgga aaagaagatt
     2101 tagtcccacg gctaaaactt cctttaatcg cggttaaaag ccggcatgca tccactcctg
     2161 gactcacagc actagccgac gaagttcgtg accgaatatt ccgcgaccca gaaattgcgc
     2221 gacgcgtaac cggttacttc aaaataagag acaatgccga agagaagaga atcgatcttc
     2281 agctcactaa agatgtttca acaccagaaa acgtcgcaat cttaaaaaag cgttttataa
     2341 tcactggcat ttcaattaac ccgattgcct attatgattt ccatgaggcc ttaatactca
     2401 attacaataa gaaatttcat cacacttaaa tgtcaaagct tcattcaagc ttaggaaaga
     2461 tcggctttgt tctccatggc gggttcatgc gaggcgcttt tcaggtgggg gcgctcaagg
     2521 ccttcgatga gttccgtctt tatccttcat atattgttgg cgaatcggtt ggcgccatta
     2581 acggagcggc atttgccgcc ggtaaagtgg aactcttgtg gaaaatctac ctagaaattg
     2641 ccaaaaatcc gcaccgctac atttatcgtt ataattttta tatgttactg cgagcctttt
     2701 t
//
LOCUS       DATDSU010000048        10306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_550024, whole genome shotgun sequence.
ACCESSION   DATDSU010000048 DATDSU010000000
VERSION     DATDSU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550024"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VI981_01560"
     CDS             <1..1009
                     /locus_tag="VI981_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479028.1"
                     /translation="NFGYIRDGVNSGVWGQWFTQTPNCQNITREDFDTYMKTPIVNWI
                     TSHPEKDIRHIVMVYMPARIDPPGGNGSVAYALNLAMRTTGIHPGGGNAFPPVIIEDG
                     HVGNGYFTRQEYPGTMALVTDLWVGSVPATKAYVDKLKSVYNSMPVKSIVISGAASGR
                     AGTSYYFEDTNSIGPNNAPFNGNLHRDAVHKINPSAPITVTGHGEPFITTASDVSGYF
                     SWGTNGGHTWPYANDGSVVFTGKSSWYPIMTYESYNGQWDSHQGDFTDWFSANAFGGT
                     NYSNTPAAAVSHVDEPSGVGVNSDLYFQCWEKGHLFIDCAYISMRTAFFMPHGDPWVT
                     K"
     gene            1125..2633
                     /locus_tag="VI981_01565"
     CDS             1125..2633
                     /locus_tag="VI981_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY4479029.1"
                     /translation="MNNFKKIVVFGVTAIFFLTGATAYAAFTQSQIDAVISLLQAFGA
                     ETSIISDVRASLSGRTTTTQPNTFCYDWNINLRVGDSNNDVVSLKNALRREGIDDVEK
                     NSSYDEEIDEKTASAIVAFQEKYRSEILTPNGLAHGTGYVGPATRRALNRRYGCSIVH
                     SGVPVITGIDAPTTLSVGQIGTWTIKAYDRENGSLSYSVRWGDEPQLSSQAEGSAEQT
                     RPFVQTATFTHSYATAGTYTAMFFVKDSTGKSASMSVTVRVGSDDQRSITVISPNGGE
                     TWPIDSKQQVRLNFTGSIPTGAVVAFSLVGGNFGAAEFYEAAYSGNSYEITIPSSVTV
                     GGDVVWPVTLGSYNLKATLKDRFSPRCSYPLPCPAGSVYPKVLAQDSSDAPFTITSAT
                     QPSITVLSPNGGESWAANSVRAITWNYANATLDSKVDLYLEFTCQPVELCEPPLPAIL
                     DKNINANGSYNWIVATDIVNNPISQGAYRMKICVAGTSNCDTGNAPFTITAN"
     gene            complement(2705..2932)
                     /locus_tag="VI981_01570"
     CDS             complement(2705..2932)
                     /locus_tag="VI981_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479030.1"
                     /translation="MSDQDTQSLLTRLKADLAEIKKANADLEANFGKYLKNDPQVSNA
                     SIQNPSVQKQEIEKSLDNDLNTLAVDFATKE"
     gene            complement(2929..6390)
                     /locus_tag="VI981_01575"
     CDS             complement(2929..6390)
                     /locus_tag="VI981_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479031.1"
                     /translation="MNGKEYEYKFVPPPPVTIGDDKKTASSLDNLAKALREKEEKAAK
                     EIAETPQPAKVEPPRPAVEAPVEPQKIEETAPKPSEKKLEEDKVKPEPPRPAPAPVPP
                     SVGTSQQSSPDSLVDKKSDVDVAATQNSIPSPTPPPPENSEAKITEVPTHEPKQEEKT
                     RGMPVKESVNEDLFDAIASELKEMAKEKVDSLKKAASEWKDKILQTIKLKTIDKWELV
                     RETRSRNVLIGLKEKEIERIRAKIEVTERQKNSLKAGLGAEMGEFDRAMSQITDLNLQ
                     AIFEKTKGARTAAYNPEALNRETEASQALIAQRTAEIATYERQVAEIEKRFSDKVDVC
                     IDAIKTRYSYDALKQEAAALESQLKQTEQILLSYREKMREYATALTFLQTKGSEAYAE
                     VNALFEELKKENATYEKNYRLLWSARDAIRLKINDAEREFGEWRKFRAEFIKTPRVSE
                     FLSRTPPDQSAPVNAPKETMPENAEQQNAPMEAPAFYRKLNESIGNLINKMRTTPKET
                     AFTTECDELVSIITNASNLELNPADREKLNKALPLLNSAKVTADLAQARIILADVVET
                     CFADLGRSESKKTVTEAAKTEIKAENNKEEELPIGKPADINVPTPPKKEEFVGRFPES
                     VGELIEEARTIAAAFTKDLNSDNIPEADERNYLNEILTRLSNAPLSREDKERVAKAKQ
                     LLGMAEAAALPDKATGKISDAATAGMYLQQVALEIANLGKRPESTIKVDPLKQEERTV
                     GGQSEGRPYTLKVLIEETKQKAEAVQRAITENKPLESSAKVLQEILAKVGEAPLPPED
                     RVKATNAKLNVEMALADPARMKGYLQSALKDLEGFGTRSQTAATKKGGSAEPRVEKLV
                     EQKMEKREGFYGFRQLQAEVVLDVGNLRKALHSDASQEKVEGLAITLNEMLARAREAP
                     LEEKDRQRVAAAKSALEKALNEKNKVSKDSLLRKAVQEMRGYAEAAQKDWTTGELTKE
                     FLAFQDLDFKDDDAKKQFLLDTLASEAGGKYAQSLKELFAAHGEMEVEELAQELQEKC
                     VEIIERSGLTASISYKSPSWAYWDLAKQFYFFTHQSEASPDYVIADPYESPGKHDEII
                     DRQLGFELFKARDKSFLAKYTKVPGYEKLHALIEENPPIKENGNLRSKDYARVGQLMR
                     LLMQAKI"
     gene            6939..8138
                     /gene="tuf"
                     /locus_tag="VI981_01580"
     CDS             6939..8138
                     /gene="tuf"
                     /locus_tag="VI981_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY4479032.1"
                     /translation="MHMAEFDRSKPHVNVGTIGHVDHGKTTLTAAITTVQSKKGLANV
                     ISYDQVAKASEKEGRRDPSKIITIAISHVEYSTENRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAVDGPMPQTREHILLAKQVGVPKIIVFLNKCDMVDDKDMLDLVEAEVR
                     ELLTKYGFDGDATPVIRGSALKAIQGDAAWEAKIGELMTALDTYIPIPVREIDKPFLM
                     PIEDIFSIEGRGTVVTGRIERGRVKVGEEVEIVGIKPTTKTTVTGVEMFNKSLQEGMA
                     GDNAGVLLRGTRKEDVTRGQVLAKGGSVTPHTEFESEVYILKKEEGGRHTPFFTGYKP
                     QFYIRTTDVTGEVTLKEGVEMVMPGDTVTFKVKLVAPIALEEKMRFAIREGGKTVGAG
                     VVTKITK"
     gene            8239..8604
                     /gene="rpsJ"
                     /locus_tag="VI981_01585"
     CDS             8239..8604
                     /gene="rpsJ"
                     /locus_tag="VI981_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY4479033.1"
                     /translation="MAKETTKKTEKAPKQPKQEVVNKLRIRVRAYEHKILDASVKQIM
                     DTALRFDAKVLGPIPLPTEIKKYTVNRSAFVYKNAREQFEMRVHKRLLDIVNPSPKII
                     EALTNLSLPSGVNIDVKMT"
     gene            8852..9529
                     /locus_tag="VI981_01590"
     CDS             8852..9529
                     /locus_tag="VI981_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479034.1"
                     /translation="MNTFTECFKTILTGEKVESRKAARRVRKLLYSSGHDRSKYDDIR
                     PLINKAPGEYAKISEDWREENFVMAVSVLYFLHHRESEPDFLFPWLFDLLQHDNGNIR
                     HAAARMFENGLGPLTYHIRCPGGGSSSNKLRIDQADRILFELYSNLQNLINDLSKPSY
                     NKYKLIASLPSGAFKSVQMVMGHLREDCGEKHMKDFNKRFSYQSLNGIELGGLCNCDI
                     CRQRKHS"
     gene            9559..9951
                     /locus_tag="VI981_01595"
     CDS             9559..9951
                     /locus_tag="VI981_01595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY4479035.1"
                     /translation="MLKRFLEWIKLKEKLHGNEKWPPPFVSERDLWWVSFGENIGSEM
                     NGKSDLFSRPALILKKLSRNFFLVVPTTSQKKEGTWYAHITHENKDMYVCLNQIRTID
                     HRRLSTRLGRIDGNDFKKVKKTFEKLYL"
BASE COUNT         2601 a         2280 c         2415 g         3010 t
ORIGIN      
        1 caacttcggt tatattcgcg acggtgtgaa tagcggtgta tggggtcagt ggtttacgca
       61 aacgcccaat tgccagaata tcacccgcga ggatttcgac acgtatatga aaacgccgat
      121 cgtgaactgg attacgtcgc atccggaaaa agatatccgg catattgtca tggtatacat
      181 gcctgccaga attgatccgc cgggcggcaa cggcagtgtc gcgtacgcgc ttaaccttgc
      241 catgcgaacg accggtattc acccgggggg cggcaatgct ttcccccccg tcatcattga
      301 agatggccat gtcggtaatg gctatttcac gcgtcaagaa tacccgggca cgatggctct
      361 tgtcaccgat ctttgggtcg gttcggttcc cgcgaccaaa gcgtacgttg ataagctgaa
      421 aagcgtgtac aacagtatgc ctgttaaaag tatcgtcatc tccggtgcgg catccggccg
      481 cgccggtacc agctactatt ttgaggatac gaacagtatt ggtcctaata acgcgccttt
      541 caacggcaat cttcaccgtg atgccgttca taaaatcaat ccgtctgcgc cgattacggt
      601 aactggacac ggtgagcctt ttatcacgac agcaagcgat gttagcggat atttctcctg
      661 gggcacaaac ggtggacata catggccata tgcgaatgat gggtcggttg tctttaccgg
      721 taaatcaagt tggtatccga tcatgaccta tgagtcgtat aacggacaat gggatagcca
      781 ccagggggac ttcaccgatt ggttttcggc gaatgccttc ggcgggacaa attattcgaa
      841 tacaccggcc gcggcggtgt cgcacgtcga tgaaccatca ggagtcggag tgaatagtga
      901 tctgtacttt cagtgctggg agaaggggca tctctttatt gattgcgcat atatcagtat
      961 gagaacggct ttctttatgc cgcacggcga tccgtgggtg acgaagtaaa ggacactcgc
     1021 acattccttt taaaatgcac tccgcctcag gcggatctgg ggcatttaaa aaggaatgtg
     1081 ctcgcttagt ttagaagtgg caaaagaaat aacaaaaaaa taatatgaat aactttaaga
     1141 aaattgttgt cttcggtgtt actgcaatat tctttttaac gggggccacc gcatatgctg
     1201 cgttcacaca aagccaaatt gatgcggtta ttagcttgct tcaggctttc ggggcagaaa
     1261 cgtcgataat ttctgatgtc agagcttcat taagcggacg gacgaccacg actcaaccaa
     1321 atacattttg ctacgactgg aacattaatt tgcgagtagg ggattccaat aatgatgtgg
     1381 tatcgcttaa gaatgcgctc cgacgggaag gaatcgacga tgttgaaaaa aatagttctt
     1441 acgatgaaga aattgacgaa aaaacggcat ccgcaattgt cgctttccaa gaaaaatacc
     1501 gtagtgaaat tttaacgccg aacggtcttg cccacggcac gggttatgtc ggtcccgcaa
     1561 cgcgtagagc gctcaaccgg agatatggat gttcaattgt tcactccgga gttccggtga
     1621 ttaccggtat tgatgcgccg acaactcttt ctgtgggaca gatcggtacg tggacaataa
     1681 aagcgtacga tcgggaaaac ggctcgctaa gttattccgt aaggtgggga gatgaaccgc
     1741 aactttcttc tcaagcggaa gggtccgccg aacaaacaag gccgtttgtt caaaccgcga
     1801 ccttcacgca ttcgtacgcc acagcgggaa catacacggc aatgtttttc gtaaaagaca
     1861 gtaccggcaa gagcgcgagt atgtcggtga cggtgcgagt tggttctgat gatcagcgct
     1921 ccatcacagt tatttctcca aacgggggag agacgtggcc aatagattca aaacagcaag
     1981 tgagattgaa tttcacggga tctattccga caggagcggt agttgcgttt agtttagtcg
     2041 ggggtaattt tggagcagcg gagttttatg aagctgccta ttcgggcaat tcttatgaaa
     2101 ttacaatccc ttcatcagta actgtgggcg gagatgttgt ctggcccgtt acacttggtt
     2161 cgtacaattt gaaagcaacc cttaaagatc ggttctctcc aagatgttct tatccgcttc
     2221 cttgtcccgc tggttctgtc taccctaaag tactggcaca ggattccagc gacgcgccat
     2281 ttacgattac ttcagctacc cagccatcca tcactgtcct ctcgccaaac ggcggagaat
     2341 cgtgggccgc aaattcggta cgcgcgatta catggaatta tgcgaacgca actctggaca
     2401 gtaaggttga tttgtatctc gaatttactt gtcagccggt tgaactttgc gagccgccac
     2461 ttccagcaat cctcgacaaa aatataaacg cgaacgggtc atataactgg attgtcgcaa
     2521 ccgatattgt taataatcca atttcacagg gtgcatatag aatgaaaatt tgcgtagcag
     2581 gtacatcgaa ttgcgataca ggcaacgcgc cgtttacgat tacggcgaac taatttaggt
     2641 tggtataacc ggagactgag tcatggttgg atctctttca ctcttacgtc ctatctagga
     2701 tacgtcattc cttagtggcg aagtcaaccg cgagggtatt caagtcgtta tctaaacttt
     2761 tttcaatctc ctgcttttgc actgaaggat tttggattga tgcgtttgaa acttggggat
     2821 cattcttcaa atattttccg aaatttgcct caagatcggc atttgccttc tttatttcgg
     2881 cgagatctgc ttttagtctc gtgagtaaac tttgtgtatc ctggtcactc atatttttgc
     2941 ttgcattaga agtctcataa gttgtccgac acgcgcatag tctttcgatc tcaaattacc
     3001 gttctctttt atgggcgggt tctcttctat aagggcgtgt aatttttcgt agccgggaac
     3061 tttcgtatat ttcgctaaaa atgatttgtc gcgagcttta aagagctcaa atcccagttg
     3121 gcgatcgatg atctcatcat gcttaccggg tgattcatat ggatcggcga ttacataatc
     3181 gggagatgcc tcgctctggt gcgtaaagaa atagaattgt tttgccaaat cccaatacgc
     3241 ccaggatggg gatttatatg aaatggaagc cgtaaggcca gatctttcga ttatctcaac
     3301 gcacttctct tgaagttcct gtgccagttc ttccacttcc atctcaccgt gcgccgcaaa
     3361 cagttccttc aatgattgcg cgtattttcc cccagcttca gaggcgagtg tgtcgagcaa
     3421 gaattgcttc ttggcgtcgt cgtctttaaa atccaaatcc tgaaatgcca gaaattcctt
     3481 cgtaagttct cccgtggtcc aatctttctg ggccgcctcc gcgtagcctc gcatctcctg
     3541 cacggccttt ctcaataatg aatctttcga gaccttgttt ttttcattaa gtgctttttc
     3601 gagcgctgat tttgcggccg caactctctg ccggtctttc tcttcaagcg gtgcttctct
     3661 ggcgcgagcg agcatctcgt ttaatgttat agcgagaccc tctacttttt cctgtgaggc
     3721 atcagagtgg agcgcttttc ttagatttcc tacatcaagt acgacttcag cttggagttg
     3781 tctaaatcca tagaaaccct cgcgtttttc cattttttgt tccactagtt tttcaactcg
     3841 cggttctgcg cttccgcctt ttttggtggc agctgtttga gatcgcgtac cgaagccttc
     3901 caaatccttg agagctgact gaaggtagcc cttcattctc gctggatcgg cgagagccat
     3961 ttcgacatta agctttgcgt tagttgcttt aactctgtct tccggaggaa gtggagcctc
     4021 gcctactttt gccagtattt cctgcagtac ttttgcgctc gattcaagcg gtttgttttc
     4081 ggtgatggcg cgttgcacgg cctcggcttt ttgttttgtt tcctcaatta atactttaag
     4141 tgtgtatggc cggccttcgg actgtccgcc aacagttctc tcctcttgct ttagtgggtc
     4201 aactttgatt gtcgactctg gtcgcttgcc caagtttgca atttcaagtg cgacctgctg
     4261 gagatacatt ccggcagtcg cggcgtcgga gatttttccg gtagctttat ccggcagtgc
     4321 ggcggcttct gccatgccga gtaactgttt agctttagcg actcgttctt tgtcctctct
     4381 actaagtggt gcgttagaaa gccgtgtcag tatttcgtta aggtaattcc tttcatcagc
     4441 ctctgggata ttgtcgctat tcaaatcttt tgtgaacgcg gcagctattg tacgtgcctc
     4501 ctcaataagc tccccgacac tttcaggaaa tcttccgaca aactcttctt tttttggtgg
     4561 tgtcggcaca tttatatctg ccggttttcc aatcggcagt tcttcctctt tattattttc
     4621 tgccttaatt tccgttttgg ctgcttctgt tacggttttt ttagattcac ttcttcccaa
     4681 atctgcaaaa caagtttcta caacgtccgc taaaataatc cgcgcctgtg cgagatcagc
     4741 cgtgactttt gcggagttca aaagcggcag agctttgttc aatttttctc tatcggccgg
     4801 attaagttca agatttgaag cgttcgtgat gatgctcaca agttcatcgc attctgtcgt
     4861 gaaagccgtt tcctttggcg tcgtcctcat cttgttaatg aggttaccga tggattcatt
     4921 taattttcta taaaatgcgg gagcctccat cggtgcattt tgctgctccg cattttcagg
     4981 catcgtttct tttggagcgt tcactggtgc actctgatcg gggggtgtgc gagagagaaa
     5041 ttcactcact ctgggagttt taatgaattc agccctgaat ttgcgccact cgccgaactc
     5101 tctctcggca tcattgatct ttaagcgtat ggcgtctcga gccgaccaga gtaatcgata
     5161 atttttttca taggttgcgt tttctttctt caattcctca aaaagagcat tcacctcggc
     5221 atatgcctca ctccctttcg tctgtaaaaa agtgagagcc gttgcgtatt cgcgcatttt
     5281 ttccctgtaa gaaagtaaaa tttgttcggt ctgcttcagt tgcgattcga gcgctgcggc
     5341 ctcttgtttg agtgcgtcat aactatagcg ggttttaata gcatcgatgc acacatcgac
     5401 tttatcggaa aaacgttttt ctatttcggc gacttgtctc tcgtaggtcg ctatttctgc
     5461 agttctctgc gcaatcagtg cttgagacgc ttcagtttcc cggttgagtg cttcagggtt
     5521 gtatgccgca gtgcgggcgc cttttgtttt ttcaaagatt gcctgtaaat tcaggtcagt
     5581 aatttggctc attgcgcgat cgaattcacc catttcagct ccgagacctg cttttagtga
     5641 gtttttttgg cgctctgtca cttcaatctt ggcgcgaata cgctcaattt ctttttcctt
     5701 taatccaatg aggacattgc gggaccttgt ttcacgcacc agctcccatt tatcaatagt
     5761 ttttaattta atcgtctgga gtattttgtc cttccactca ctcgcggcct ttttaagcga
     5821 gtccactttc tctttagcca tttctttcaa ctctgaggct attgcgtcaa acaaatcttc
     5881 gttgacactt tcttttactg gcatacctct cgtcttttct tcttgtttcg gttcgtgtgt
     5941 tggaacttct gtgattttgg cttccgaatt ctctggcggt ggcggcgtgg gacttggtat
     6001 ggaattttgt gttgcggcta cgtcaacatc actcttttta tctacaagag aatctgggct
     6061 actttgttgt gatgtgccaa cgctcggagg aactggagct ggggcgggtc ttggcggctc
     6121 gggttttacc ttgtcttcct ccagtttttt ctccgaaggt tttggtgctg tctcttcaat
     6181 tttttgcggc tcaacgggtg cttcaacggc aggacgggga ggttcgactt ttgcgggttg
     6241 cggcgtctcg gcaatctctt tagcggcctt ttcctctttt tcccttaatg ccttagccaa
     6301 attgtccaaa cttgaagcag ttttcttatc gtccccgatt gttacaggtg gcggcggaac
     6361 aaatttatat tcgtattctt tgccattcat cccgttagag ataggaaatg cgcgtgctta
     6421 ttcacagcac atttgctatt gatggttacc gctatttttg gtgctttact cattaaaata
     6481 aaaatgcatt tccgtagcac ctcggtgcaa ctacacttaa tgactgaagt acacctatgt
     6541 agtattaaag tctgaatcat aacaactaga tctctaacgg gattcatccc gttagagacc
     6601 cgcctgccat cgcctgacac actgatggaa ggacgtttga ttaattggta atgtcattat
     6661 catttttaca gacttaacga tggcattatt gtatcacgcg gactgctggt tttttgaaac
     6721 ctggggaaac tttaagtaat ttttctcatc tgatttgact cttttaagca ctctggtaag
     6781 atgactaaat cgctttttgc gtgtctgatt tggaacttgt gctacgttct catgtatcac
     6841 aagagggatt ttcccgccgg atcgggcaat tattaacttt ttaagaaatg ggaatgagcc
     6901 gttctcgcgc gttcgttcgc ttttcttaag ataaatatat tcatatggca gaatttgatc
     6961 gatcaaagcc ccatgtcaac gtcggtacaa ttggccacgt cgaccatggg aagaccactc
     7021 taactgcggc aatcacaact gtgcagtcaa agaaaggtct tgctaacgtt attagctatg
     7081 accaggttgc aaaggcttct gaaaaagagg gccggcgcga tccgtcgaaa attattacga
     7141 ttgcgatttc tcacgtcgag tattcgacag agaatcgtca ctacgcacac gttgactgtc
     7201 ccggacacgc tgactatgta aaaaacatga tcaccggtgc ggctcaaatg gacggcgcaa
     7261 ttctcgttgt ctctgcagtt gatggcccga tgcctcaaac acgagagcat attcttcttg
     7321 cgaaacaggt cggcgtacca aaaatcattg ttttcttgaa taagtgcgac atggtagatg
     7381 acaaggacat gcttgatctc gtcgaagcag aagtacgaga gcttctcact aaatatggat
     7441 ttgacggaga tgcaacgcct gttattcgcg gatcagctct aaaagcaatt caaggtgacg
     7501 ctgcgtggga ggctaaaatt ggggagctta tgacggcgct agacacctat atccctattc
     7561 cggttcgtga gatagataag cccttcctca tgccgattga agacattttc tcgattgaag
     7621 gccgcggaac agtggtaacc ggaaggattg aacgaggacg agtgaaagtc ggcgaggaag
     7681 ttgaaatcgt aggtatcaaa ccaaccacga aaacaacagt gacgggagtt gaaatgttca
     7741 acaagtcact ccaggaagga atggccggag acaatgccgg tgttctcttg cgcggtacgc
     7801 ggaaagaaga tgtaacccgc ggacaggtcc tcgcaaaagg cggctcggtg acgccccaca
     7861 cagaatttga gtcagaagtc tacattctta aaaaagagga aggtggccga cacacgccgt
     7921 tctttacagg ttacaaaccc cagttctata ttcgaaccac agacgttact ggtgaagtga
     7981 cacttaaaga gggagtggaa atggttatgc ccggcgatac cgttactttc aaagtgaagc
     8041 ttgttgcacc gatcgctctt gaggaaaaga tgcgatttgc gattcgcgaa ggaggcaaga
     8101 ctgtcggtgc cggagttgtt actaagatca ctaagtagaa agtatcgatg cgaatatgcg
     8161 aattatgcga atgcaacgaa tggacttcta ttcgtatcat tcgtacctat tagtagatta
     8221 gtatcgatac agcgaaaaat ggctaaagaa actacaaaaa aaacggaaaa ggcacccaaa
     8281 cagcccaaac aggaggtagt caacaaactt cgcatcaggg ttcgagccta tgaacacaaa
     8341 atactcgatg cttcggtgaa gcagatcatg gacacggcgc ttcggttcga cgcgaaggtg
     8401 ctcgggccga ttccgcttcc gacggagata aaaaaatata cagtcaaccg ttccgctttt
     8461 gtttataaaa atgcgcgaga acaatttgaa atgagagtgc ataaacggtt gctcgatatt
     8521 gtgaatccgt caccaaaaat aatcgaggcg cttacaaact tgagtttgcc atcgggggtg
     8581 aatattgacg tgaagatgac gtaaaggacg ctcggattcc ttctctttca aaacacgtat
     8641 aattttctgc tgaaaattta ctatgctcgg cccgtcggcc ttgcgcaatg ttttgaaata
     8701 gaagaatcct cgctccgatg aaggagaagg aaagagaaaa atcccccgca tggcggggga
     8761 ttttgttgga aaaaagttcg gattttgttc aggagacaca gccaattaag ttcgattgta
     8821 aacattttta tttgtggtat atatcaatga tatgaatact tttacggaat gtttcaagac
     8881 aatattgact ggagaaaagg ttgaaagtcg aaaagcggcg cgacgggtta gaaaattact
     8941 atacagttct ggtcacgacc gaagcaaata cgacgacatt aggccgttga taaataaagc
     9001 gccgggggag tacgcaaaga tttccgagga ctggcgtgaa gagaacttcg ttatggcggt
     9061 atccgttctg tattttttgc atcacaggga gagtgaacct gactttctat tcccatggct
     9121 ttttgatctg cttcaacatg ataacggtaa catccgccat gccgctgcac gtatgtttga
     9181 gaacgggctt ggaccgctca catatcatat tcgttgtccg ggtgggggat cgtcgtccaa
     9241 caagttaagg attgatcagg ccgatcgaat cttattcgag ttatactcca acttacagaa
     9301 tttgatcaat gacctctcga agccctctta taataaatat aaactaatag cttctctacc
     9361 gagtggagcg tttaagtcgg tccaaatggt tatgggtcat ctaagagaag attgtggaga
     9421 gaaacatatg aaagatttta acaaaagatt tagttatcaa tctctaaatg gaatcgaact
     9481 tggagggttg tgtaactgcg atatatgcag acaaagaaaa cattcttgaa atgataatta
     9541 tgtggtaaaa tcttttttat gttaaaaaga tttttggagt ggataaaact taaagagaaa
     9601 ctccatggaa acgaaaagtg gccacccccg ttcgtgagtg agagagattt gtggtgggtc
     9661 agttttggag aaaatatagg ttcagagatg aatggcaaga gtgatctatt ttctagaccc
     9721 gcattgatct tgaaaaaact atctcgaaac ttctttttgg tagtaccgac gacttcgcaa
     9781 aagaaagagg gtacatggta tgcgcatata acgcacgaaa acaaagatat gtacgtctgt
     9841 ctcaatcaga ttaggactat agaccataga agattatcga cacgcctggg aagaattgat
     9901 ggtaatgact tcaagaaagt taagaagacc ttcgaaaagc tatatctata aaaatattcc
     9961 ccgcttttta ggcggggccg cggcgaatgc cgaatgtagt cacatgatag caaatcaagg
    10021 tgctatgtca atggctcttt tcagagtacg gcttaaatcc gttcggcccc gaaaatcatc
    10081 gaggccttga ccaatctttc attgccgact ggagtgaata ttgatgtgaa gatgacctag
    10141 tagatgcaga ctggatgcgg atttaataat tactttgaat tacgaaactc ccttttccca
    10201 cattttcata ttaaaataca aatggatgcc tttaggcatc catttgtatg aatacttcta
    10261 tcaaaccgta caactccata ctaacaaaag ttacttttct catcaa
//
LOCUS       DATDSU010000049        12549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_550264, whole genome shotgun sequence.
ACCESSION   DATDSU010000049 DATDSU010000000
VERSION     DATDSU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550264"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VI981_01600"
     CDS             <1..630
                     /locus_tag="VI981_01600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY4479036.1"
                     /translation="EDRLHPEPRKTILFTFLAGMLVVPFARQLEQLAWEVIRPESFLL
                     VVIWSGIEELLKFFAAFFPAILSKYDDEPVDAVIYMITAALGFAAYENALYLANPDFG
                     TTFISTLLTGNLRFMGATLLHVISSASIGMALGFAFFRKDASKITHWLIGFFIALTLH
                     SFFNFFIMGSSGQSVFIVFYAVWVAILVLFFFFEKLKKVGIKPDNQITS"
     gene            640..1092
                     /locus_tag="VI981_01605"
     CDS             640..1092
                     /locus_tag="VI981_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY4479037.1"
                     /translation="MAKKKSFFERLTGTVKVDDDEIEEDTRGKAGDENWIEEEAAEGQ
                     LTVDVHQTSNDIIVKAMVAGVLPEDLDVSITRDMVTIKGKREESHEIKDDDYFYKELY
                     WGTFSRTIMLPQEVDVEEAEATENHGLLTIRLPKINKERQAKLKVKSS"
     gene            complement(1093..2079)
                     /locus_tag="VI981_01610"
     CDS             complement(1093..2079)
                     /locus_tag="VI981_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479038.1"
                     /translation="MFGVDLYKKLHRQYYGVKSVHLLIALLSVAILAEAWDTWWHRAI
                     GHDTFFDPPHTLLYVSVVLALIVAFYLWRWGHVANSKYIFLSLLLLPISVPLDELWQG
                     LFSVGNSTNSLIIWAPMRLLVVFGITAAMLLLLSVLRHERKRLDHWLFMVLGFGSLLW
                     LGFFVLIPLSPYGPFHIIGLWGTVFVSFVFCGVLLFAQRFIKGPEEVLPITVIFLLML
                     MVGTFEGVSSSIYIPSFGFAESPHWLTAFTFLLAALSIEVLKKIPPSLSGAIAGFVWA
                     LPFYLVAPYFTGGDMLYSNILSIAIAVLSSVVGGLAAGIAAKMLIRGHFGQN"
     gene            complement(2205..2276)
                     /locus_tag="VI981_01615"
     tRNA            complement(2205..2276)
                     /locus_tag="VI981_01615"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2242..2244),aa:Phe,seq:gaa)
     gene            complement(2439..2663)
                     /locus_tag="VI981_01620"
     CDS             complement(2439..2663)
                     /locus_tag="VI981_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479039.1"
                     /translation="MTPEEKSILLKTQELALENNKILRSMQRNSRWSMFFRVIYWTAL
                     IVIAGGTYYFLQPYLEQLLKVYGSASNLFD"
     gene            complement(2839..2910)
                     /locus_tag="VI981_01625"
     tRNA            complement(2839..2910)
                     /locus_tag="VI981_01625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2875..2877),aa:Arg,seq:cct)
     gene            complement(2923..3546)
                     /locus_tag="VI981_01630"
     CDS             complement(2923..3546)
                     /locus_tag="VI981_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479040.1"
                     /translation="MRVHGVSKIRELHKMRRKGHSIQDLMKLFSLSKSTVWHHIHSIP
                     LTASQKKLLRSRQGGGAKYKELRWQQARIQAKELLSGPYRELVIIVAMLYWAEGHKQR
                     DCHFTNSDGRMVTMYLFVLRRVFLIKNDAIHPILRIFTGMDEMECLIYWSQATGIPKE
                     KFAVLLNDGGIRGRTTHGICRIVVRKGTQTLKLIHSLIDQIFKEVIR"
     gene            complement(3697..3942)
                     /locus_tag="VI981_01635"
     CDS             complement(3697..3942)
                     /locus_tag="VI981_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEY4479041.1"
                     /translation="MAEQKKVTIYSTPTCTYCNAAKEFFKKNNISYAEHNVGTDLEKR
                     KEMVEKSGQLGVPVIDIGGEVIVGFDKPRISGALGVS"
     gene            4051..4124
                     /locus_tag="VI981_01640"
     tRNA            4051..4124
                     /locus_tag="VI981_01640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4083..4085,aa:Gly,seq:ccc)
     gene            complement(4134..4493)
                     /locus_tag="VI981_01645"
     CDS             complement(4134..4493)
                     /locus_tag="VI981_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY4479042.1"
                     /translation="MREFEAKRRFKRLLYSKLSILLLFLLLLFAGHSTWKLYAKQRQS
                     ERKREEVERELAELQAREKLLFREIEDLKSPEGVEREIRDKFRMAKEGEELVVIIEDK
                     STTTHRTSSKKWWQFWK"
     gene            complement(4815..6599)
                     /gene="lepA"
                     /locus_tag="VI981_01650"
     CDS             complement(4815..6599)
                     /gene="lepA"
                     /locus_tag="VI981_01650"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY4479043.1"
                     /translation="MTAQKLIRNFVIMAHIDHGKSTLADRMLEITGTVEKRKMKDQML
                     DNMELERERGITIKMQPVRMNYTLDAIPYTLNLIDTPGHVDFYYEVSRALKAVEGAIL
                     LVDATQGVQAQTLSTLSMAEEAGIKIIPVVSKIDSPLARVSDVKEELAKLLGCSVESV
                     LECSGKTGVGVENLLKQVIKQIPAPESQSDKFRALIFDFQYSNHRGVIVYVRVMDGKA
                     SRNQNFLFKQTAAKFISLEVGIFLPEEKSVEEISAGEIGYIITGIKQPGIASVGDTIT
                     EFKDPMPALAGYMSPKPVVWASVYPESQDDFNALRQALARLRLSDSSFTHEEESSGIL
                     GRGFRAGFLGMLHLEIITERLRREFGLSLIVTHPSISYEVRTKNGETKIIYTPTHFPE
                     YGEIASVREPWISLQLITPPDYLGPIMQMLYDHEAEVGESESFGENRTRIFVKMPLRE
                     LMRNFFDRIKSVSSGFASISYKIEDFRDADVVRLDLLVADELVPAFSRIVSRRKVEHE
                     AERAVEKLHTILPRQLFVTKIQGRALGRIISSKTLSALRKDVTGYLYGGDVTRKMKLL
                     EKQKKGKKKMKAQGKVDIGQDVFVKMMH"
     gene            complement(6782..8461)
                     /locus_tag="VI981_01655"
     CDS             complement(6782..8461)
                     /locus_tag="VI981_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY4479044.1"
                     /translation="MVKRLFSFLGQEMSGLHEAAYLLALFAILSQVAGFIRDRLLAHL
                     YGAGQELDVYYAAFRIPDFIFVSVASLVSASVLVPFIISRQHGDQAEAKKFIDSIFSF
                     FFFFIVIVCVFLFILMPFIAHVIFPGITDPHLAGKLVSYARILLISPVLLGLSNFYSS
                     IAQAKHRFFSYAISPVLYNIGIIVGVVFFTPMWGLSGVVYGVILGAVLHVLVQIPALA
                     REKIFPRFHFKFSLAPVREVVLLSFPRTLALSSSHLSMIFLVAYASLMTEGSISVFNF
                     AFNLQSVPLSIIGVSYSVAAFPTLSRLFGNGERDKFLSEILTAARHIIFWSVPITVLF
                     VVLRAQVVRTVLGSGNFSWADTRLTAAALALFAISVLGQSLILLFVRGYYAASNTKKP
                     LYINMFSSVFIIVFAMLFLKLFYANELFQRFIEALLRVQNISGTAVLMLPFAFTIGIL
                     LNVSLLWIVFERDFGKMGHVIFKTLFQTLGASLIMGYVSYEMLRVFDNVFDLSTLPGI
                     FLQGLCAGLVGIFVGIVILVLLKNEETRVIWKTLHSKIWKAETIVPPQENL"
     gene            complement(8464..10860)
                     /locus_tag="VI981_01660"
     CDS             complement(8464..10860)
                     /locus_tag="VI981_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4479045.1"
                     /translation="MEKETSERVSVGILEKISLWIFLALVFFLPFFFLKSVAFQLSKS
                     LFIAITVITMLIVWSAWKLKEGKISYFNTITTWGALAVIVSTVLSTFFSDSIARSFIG
                     QGFEIGTLSAVIISFALLFLIPLTVKNEARIFYFYATFIASLIVFALYHMVRLFAGPD
                     VLSFGLFGNAAASTLGKWNDVGLFFGAGAIMGLFAVEFLSFSPLVKFISWVTLIVSLF
                     FLALINFTAVWIVIGLIAIVSFIYGAWFRKVAKPKSETSGKKFVQKISFVSLAVVILA
                     IIFALGRNGVGMRLSDSFDVAQLEVRPNWSSTLHIVKAALKEDPIFGVGPNRFVNTWL
                     LNKPVEVNETLFWSTDFNSGVGTIPTFIAQTGILGILAWLLFLGGYLYIGIRGILSTP
                     KSALARYFTASSFLVSLYLWVFNVIYVPSHVMHILTFVFTGVFLAALANEKMLAVKDV
                     EYSKVPRLNFAAVLVLVLLLIGSASLGYLYMQKYLSTSYISKGIKILNSSGDLALAER
                     SIVKGINISLREDEFYRIYSQLAILKLRNLLNEQNISQDDLRSRFQGILTDALNAGQR
                     AVRVDPKAYQNWTALAQIYEVLVPLNIDGAYDNAKSAYESALKLNPQSPSLYLLLARL
                     EVTKGNTTTARTYINKALALKSNYTEAVFFLSQLEVAANNMKAAIIQSEKAVKLAPND
                     ATLLFQLGFLKYSDKDYTSAITSLNQAVAQNSSYSNARYFLGLSLYKMNQTNEAIAQF
                     VEIEKLNTDNQEVKLILSNLRAGRDLFANSRAATDAPEKRKNLPVDESVEAEESSVAE
                     "
     gene            complement(12034..12321)
                     /locus_tag="VI981_01665"
     CDS             complement(12034..12321)
                     /locus_tag="VI981_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479046.1"
                     /translation="MPYVKVFSSKKKKGVLMALTSERVGQIAMVAFQSRKEEEGVRLN
                     PKQVKRDISNEARKLGIPVHEAAQFVQLMFREAYDKTMAELDELIPIKDRT"
BASE COUNT         3723 a         2814 c         2710 g         3302 t
ORIGIN      
        1 gaggatcggc tgcaccccga accgcgcaag acgatactgt ttacgttcct cgccggcatg
       61 ttagtcgttc cgtttgcgcg acaattggaa caacttgcct gggaagttat acgaccggaa
      121 tcctttctgc ttgtggttat ctggtcaggc attgaagaac ttttaaaatt ttttgcggct
      181 ttcttcccgg ccatactttc aaaatacgac gacgagccgg tcgacgcggt gatttacatg
      241 attacagccg cactcgggtt tgcggcgtac gagaatgcgc tatatctcgc caatcctgat
      301 ttcggtacaa cgttcatatc cacgcttttg accggcaatc tgcggttcat gggtgcaact
      361 ctactccatg taatctcctc tgcttccatt ggtatggccc tagggtttgc ttttttccgc
      421 aaggatgcaa gcaagatcac ccattggctc attggtttct ttatagcgct tacattgcac
      481 tcgtttttca atttctttat aatgggttca agcgggcaga gcgtttttat agtattttac
      541 gcagtttggg tcgctatact cgtcttattc ttcttttttg aaaaattaaa gaaagttggg
      601 ataaaacctg acaatcagat tacaagttaa tttaggaata tggcaaaaaa gaaatctttt
      661 ttcgaacgac tgactgggac ggtcaaggtt gacgatgacg aaattgagga agatacgagg
      721 ggtaaggcgg gagacgaaaa ttggatcgag gaggaagcag cagaaggcca gcttactgtc
      781 gacgttcacc aaacttcaaa tgacatcata gtaaaagcca tggtcgcagg cgtattgcct
      841 gaagacttgg atgtttcgat cacgcgagat atggtcacaa ttaaaggcaa gcgcgaggaa
      901 tctcacgaga taaaagatga cgattatttt tataaagaac tgtattgggg tacattttcg
      961 cgcacgatta tgcttcccca ggaagtggac gtggaagaag ccgaggcaac agaaaaccac
     1021 ggactgctga caatccgatt gcccaaaata aataaagagc gacaggcaaa acttaaagta
     1081 aaaagctcct aatcaatttt ggccaaagtg gcccctaata agcatcttgg cagcaatacc
     1141 tgccgcaaga cctccgacaa cactcgaaag cacggcgatc gcgatcgaga gtatattgct
     1201 atataacata tcacctcccg taaaatacgg ggcaacaaga taaaaaggca gtgcccagac
     1261 aaaaccggct attgcgcccg agagcgaagg gggaattttt tttaaaactt caatagaaag
     1321 cgccgctaaa agaaaagtga acgcagttaa ccaatgtgga gattctgcaa aaccaaaact
     1381 gggaatataa atcgatgatg aaactccctc aaacgttccg accatcagca taaggagaaa
     1441 aattacggta atcggcaaaa cctcttctgg cccttttata aaacgctgtg caaaaagaag
     1501 tactccgcaa aagacgaagg aaacaaaaac agtaccccaa aggccgatta tatgaaatgg
     1561 gccatacggc gatagtggaa ttaacacaaa aaaaccaagc cacaacagag agccgaatcc
     1621 cagaaccata aagagccaat gatcgagacg tttccgttca tgacgtaaaa cgcttagcaa
     1681 aagaagcatt gccgcggtaa taccaaacac gacgagtaac cgcatgggcg cccatataat
     1741 aagcgaattt gtactatttc cgacactaaa taaaccctgc cacaactcat caagcggcac
     1801 tgaaataggc agcaatagaa gtgacagaaa aatgtattta ctattggcca catggcccca
     1861 tctccaaagg tagaaagcga caatgagcgc aaggacaact gacacgtaga gaagcgtgtg
     1921 tggtgggtca aaaaacgtgt catgcccgat agctctgtgc caccaggtat cccaagcttc
     1981 ggctagaatt gcaaccgaaa gcagcgcaat caacaaatgc acacttttta caccgtagta
     2041 ttgacgatgc aattttttat aaaggtccac gccgaacatg aaatacatta tatcctagca
     2101 tacacaacag gttatcccct tctaagcaaa ccccgttaga agctcgcaga tcgcggcctc
     2161 taacggggta aacagggggc tttatcttcc agattctatc ttagtgccga ggggcaggat
     2221 tgaactgccg acctaccgct cttcaggcgg acgctctacc actgagctac ctcggcattt
     2281 tttgttctgg tttttccaga acaaaataat gatggcgtac taaaaagtac tactacctgc
     2341 cccgtagtga tcatttttat gatctactat gcagagctac gtcgctccaa actactctat
     2401 cgatttaaaa tcattcaggc aagtgagaaa aatatccgct aatcaaagag gttcgaggca
     2461 ctcccgtaaa ctttcagtag ttgctcaaga tacggttgaa gaaaataata ggtacctccg
     2521 gcaattacga tgagagcagt ccagtagata actcgaaaaa acatactcca acgactgttt
     2581 cgctgcatgc ttcgcagtat tttattgttt tcgagcgcga gctcctgtgt ctttagcaaa
     2641 attgatttct cttctggagt catctgaaaa gtataaagta tcaggtgtta agtataaagg
     2701 gaaaattgtg cccccggcag gaatctcgac taacgtcgct ggtgcttttc catctcgaag
     2761 actcgatacg gaaaagtctt tcgattcttg ccgctagcta agcatctggc tcgcacgctg
     2821 tcatctcatt ttattcgatg ccaccggcag gaatcgaacc tgcatttaga gattaggaac
     2881 ccctcgttct gtccattgaa ctacggcggc tggtagaatc atctatctta taacttcttt
     2941 gaatatttgg tcaattaatg aatgaatgag tttaagcgtt tgagttcctt ttcttacaac
     3001 aattctgcaa attccatggg ttgttcttcc tcgtataccc ccatcattta aaagtaccgc
     3061 gaacttttcc tttggaattc cggtcgcctg tgaccaatag attaagcatt ccatctcatc
     3121 cattcctgta aaaatacgga gtatggggtg tatagcatca tttttaatca aaaaaactct
     3181 ccgcagtaca aatagataca tagtcaccat ccttccatca gaatttgtaa aatggcaatc
     3241 acgttgtttg tgaccctctg cccaatacag cattgcaact ataattacca actctctgta
     3301 tgggccagat aaaagttctt ttgcctgtat tcgagcttgc tgccaacgta attctttgta
     3361 cttggcgcca cccccctgtc gagatcttaa aagtttcttc tgggaagcgg ttaacggaat
     3421 tgagtgaata tgatgccaaa ctgtcgattt tgaaagactg aaaagtttca ttaggtcttg
     3481 aatgctgtga cctttacgcc gcattttatg taattctcga atttttgaaa ccccatgtac
     3541 tctcattgga agtattgtat cacaatcaga tatattaaat agtcgtggct gtgtataaca
     3601 aaggttcaac taagtgtaaa tccgaaaata tacccaaaag caacagggat cgattttttt
     3661 cgaaacaagg tcaaacttta tttttaaaat attcccttag gagacaccca aagcgcccga
     3721 gatacggggt ttatcaaagc cgacaatgac ctcaccgccg atatcaatga cggggactcc
     3781 gagctggccg cttttttcaa ccatttcctt tcgtttttct aaatctgtac cgacattatg
     3841 ttctgcgtat gagatgttat ttttcttaaa aaattcttta gccgcgttac agtacgtgca
     3901 tgtcggcgtt gagtaaattg tgactttttt ttgttcggcc atagttatta aaattaatgt
     3961 ctcgatatta taacagacac aattgttata gcaaacatag atgttcatct atatttcacc
     4021 tcatacaatt catgctagga taatgaaaac gcggaattag tatagtggca atatgcgtgc
     4081 ttcccaagca tgatacgcgg gtccgattcc cgcattccgc accaagttag aacctatttc
     4141 caaaactgcc accatttttt ggagctggtt cgatgtgttg ttgtactttt gtcctcgatg
     4201 attacgacaa gctcctcccc ttcttttgcc attctgaatt tgtcgcgtat ttcacgttct
     4261 acgccttccg gacttttcaa atcttcgatt tcccgaaaca gcagtttttc acgcgcctgc
     4321 aactctgcaa gctctcgttc gacttcttct cgttttctct cactttgacg ctgttttgca
     4381 tagagtttcc acgtagaatg gccggcaaag agcaaaagca aaaaaagcag caggatcgag
     4441 agcttagaat acaggagtcg tttaaatcgt ctttttgcct caaattcgcg cacaagagaa
     4501 gggtttaacg tgtagggtgt aggatgttgg tttcatattt atcattggaa aattccactt
     4561 tgatacctta tactttacca ttatacgtca aacttctgtg ctatttcacc cccgaaataa
     4621 aaaaatattc aaagtattct ggatcgtttt atgcgtgctc atcattgtct caatggtagc
     4681 gatgtatatt ccgagcctga caaactgact tgcagcctat agcgtgtagg gtttagcgta
     4741 tagtctcgaa aatactcgcc gactgtctat cgtgtataag ctaaaagcta tcagttgaat
     4801 gctacaagct aaccttagtg catcattttg acgaatacgt cttgcccgat atccaccttg
     4861 ccctgtgctt tcatcttttt ctttcctttt ttctgttttt ccaataattt catttttcga
     4921 gtaacatcgc caccatatag ataccccgtt acgtctttac gcaaggcgct taacgttttt
     4981 gaagaaataa tgcggccaag agcgcgcccc tggattttag taacaaaaag ttggcgcggc
     5041 aaaatagtat gaagtttctc tacggcccgc tctgcttcat gttcgacttt ccgacgagat
     5101 acgattcttg aaaatgccgg caccaattca tctgcgacaa gaaggtcaag acgcacaaca
     5161 tcggcatccc gaaaatcttc aatcttgtac gaaattgacg caaaaccgga ggagacactt
     5221 ttaatgcggt caaaaaagtt cctcatcaat tcacgaagcg gcatttttac aaagatgcga
     5281 gtccggtttt cgccaaaact ctccgactcg cccacttctg cctcgtgatc gtacaacatc
     5341 tgcatgatag gacctaaata gtcgggcgga gttatgagtt ggagtgaaat ccacggctca
     5401 cgaacacttg cgatctctcc atattccgga aaatgcgtcg gtgtatagat gatctttgtc
     5461 tcgccatttt ttgttctcac ctcgtagctt atcgacgggt gcgtcacaat aagcgagagt
     5521 ccgaattccc gacgtagacg ctcagtgatg atttcaagat gcagcatacc cagaaaaccg
     5581 gcgcgaaacc ctcggccgag aattccggac gactcttctt catgagtaaa ggacgagtct
     5641 gaaagccgaa gccgcgcgag cgcctgtcgc agagcattga aatcatcttg gctctcgggg
     5701 tacaccgagg cccacacaac tggcttcgga gacatatagc cggcgagagc cggcattgga
     5761 tccttgaatt ccgtaatcgt gtcgcccacg gatgcaattc ctggctgttt aattcccgtt
     5821 atgatgtagc ctatttcacc tgcacttatt tcctcaaccg atttttcttc aggcaaaaaa
     5881 atgccaactt caagcgatat aaatttagct gcagtctgtt taaatagaaa gttttgattt
     5941 cgtgaagcct taccgtccat aacgcgaaca tagacaatta ctccccgatg gtttgaatat
     6001 tgaaagtcaa aaataagtgc tcgaaattta tcactctgac tctctggtgc aggaatctgc
     6061 tttatgactt gtttcaagag attttccacc ccaacgcctg ttttccctga acactcgagt
     6121 acgctctcga ctgaacatcc gagcagtttg gcaagttcct ctttaacatc cgacacgcgg
     6181 gcaagcggag agtcgatttt gcttaccaca ggaattatct taatgccggc ttcctccgcc
     6241 atggaaaggg tcgatagagt ctgcgcctgc acgccttgag tcgcgtctac gagcagaata
     6301 gcgccttcga cggccttgag cgcgcgagac acctcatagt agaagtcgac atggcctggt
     6361 gtgtctatca aattgagcgt atagggtata gcgtctaggg tatagttcat tcggactggt
     6421 tgcattttga tcgtaattcc ccgttcacgc tcaagttcca tattgtcgag catttgatcc
     6481 ttcatcttgc gtttttcaac agtgccggtt atctcaagca tgcgatcagc gagagtagac
     6541 ttaccgtgat caatatgagc cattattaca aagttcctaa tgagtttttg agctgtcatg
     6601 gagagatact agcagaaata ccctgaaaga taaaaaaaca aaaaaaccac cttaagagat
     6661 ggctaacaag gcagaaagga acagcggcga cggacagata aaatctaaag cattagattt
     6721 tcgtgcattt taaaaagaat atgcgagccg agcaccagaa tggtgcttcg gcgagcgccc
     6781 ttcaaagatt ttcctgggga gggacaatcg tttctgcttt ccagattttt gaatgcaatg
     6841 ttttccagat cactcgagtt tcttcgtttt tgagcaacac aagtattacg attcccacga
     6901 aaatacccac cagtccggcg cagagtccct gaagaaaaat cccgggaagc gtcgagagat
     6961 cgaagacgtt gtcgaatact cggagcattt cataagagac gtaccccatg ataagcgacg
     7021 ccccgagtgt ctgaaacagc gtcttaaaaa taacatgtcc catttttcca aaatctcttt
     7081 caaaaacgat ccaaagcaga gagacattca agaggatgcc gatggtaaag gcaaaaggca
     7141 acataagtac agcagtgcca ctaatgtttt gaacgcggag taacgcttcg ataaaacgct
     7201 ggaatagctc gtttgcataa aagagcttga ggaaaagcat cgcaaaaaca attataaaaa
     7261 ctgacgaaaa catatttatg tagagcggtt tttttgtgtt cgaggcggca tagtagccgc
     7321 ggacaaataa aagaatcaga ctttgtccca atactgaaat agcaaagagc gcaagcgcag
     7381 cggccgtgag acgcgtatcg gcccagctga aattgcccga tccaagaacc gtgcgcacca
     7441 cttgagcgcg gagcacaacg aataaaactg ttatgggcac agaccaaaaa ataatgtggc
     7501 gggcagcggt gagaatttcg cttaggaact tgtcccgttc cccatttcca aaaagccgcg
     7561 agagtgtcgg aaacgcagcc actgagtatg acacgccgat aattgagagc ggtaccgatt
     7621 gcaaattaaa ggcaaaattg aaaacggaaa tcgaaccttc agtcatgagc gatgcgtagg
     7681 cgaccaaaaa aatcatggac aaatgactcg aagagagcgc cagagtccgc gggaaagaaa
     7741 gcaaaacaac ttcgcgcacg ggcgctaatg aaaatttaaa atgaaaacgc ggaaaaatct
     7801 tttcgcgagc caaagcggga atctgaacaa gaacatgtaa gactgctccg agaatcacgc
     7861 cgtagacgac tcccgagagt ccccacattg gtgtaaaaaa aacgactcca actataatcc
     7921 caatgttata cagtacgggt gaaatagcgt atgaaaaaaa acgatgcttg gcttgagcga
     7981 tactcgaata aaaattggac agaccaagca agacaggaga aatgagcaaa atacgcgcat
     8041 aggaaaccag ttttccggct aggtggggat cagtgatacc cggaaaaata acatgcgcaa
     8101 taaatggcat aagtataaac agaaaaacgc atacgatcac gataaaaaag aagaagaaac
     8161 tgaaaatact gtcgataaac ttcttggcct ctgcttggtc accatgctgg cgcgaaataa
     8221 taaagggcac aagcacggac gcagatacaa gcgacgccac ggaaacaaaa ataaaatccg
     8281 ggattcgaaa agccgcgtaa taaacatcaa gctcctgtcc ggcgccatag aggtgcgcaa
     8341 gaagcctatc acgtatgaat cccgcgactt gggaaagaat ggcaaaaaga gccaagaggt
     8401 aagcggcctc atggagaccg ctcatttcct gtcctaagaa tgagaagagt cgctttacca
     8461 tcgttattcc gcaacggagc tctcttcagc ctcgacagac tcatcaacgg gaagattttt
     8521 acgcttttcc ggcgcgtctg tcgctgctcg cgagtttgca aatagatcgc ggccagcgcg
     8581 aagatttgag agaatcagtt tcacttcctg gttatctgta ttcagctttt ctatttcaac
     8641 gaattgtgct attgcctcgt ttgtctgatt cattttgtac aaactaagcc cgaggaaata
     8701 gcgcgcgttc gagtacgaac tattttgggc tactgcctga ttgagtgagg ttattgcgct
     8761 tgtgtaatct ttatcggaat atttcagaaa tccaagttga aagagaagtg ttgcatcatt
     8821 cggagcaagc ttgacagctt tttctgactg gattattgcg gctttcatgt tattcgcagc
     8881 tacttcaagt tgcgagagga aaaatacagc ttcagtgtaa tttgatttta gagcaagtgc
     8941 cttgtttata tacgtacgag cggtagtcgt attgcctttt gtcacctcca gtcgagcgag
     9001 aagaagatac aggctagggc tttgcggatt taatttcagc gccgattcat acgccgattt
     9061 cgcattatca tacgctccat cgatgttcag cggtacgaga acttcataaa tctgagctaa
     9121 tgccgtccag ttctgatagg cctttggatc aacccgaacc gcacgttggc cggcatttaa
     9181 ggcgtcagta agtattccct gaaaacgcga gcgtagatcg tcttgagaaa tattttgctc
     9241 attcaaaaga tttcgaagct tgagaatggc taattgacta taaatacggt aaaactcatc
     9301 ttctctcaag cttatattga tacctttgac aatagatcgc tcggcgaggg ccagatctcc
     9361 tgatgaattg agaattttga tgccctttga aatgtacgat gtgctcaagt atttttgcat
     9421 gtacaaatat ccaagtgagg ccgagccaat gagtaggagc accagcacaa gaactgcggc
     9481 gaaattgagt cgaggcactt tgctgtattc tacgtctttt acggccagca ttttttcatt
     9541 tgccagagct gcgagaaaaa cacccgtaaa gacgaaagtt agaatatgca tgacatgact
     9601 cgggacgtag ataacgttaa acacccacag atagagagaa accagaaaag atgaggctgt
     9661 aaagtaccga gcgagcgcac ttttcggagt cgagagaata ccgcgaattc cgatatagag
     9721 gtatcctcct aaaaatagta gccacgcaag aatgcctaaa attccggttt gtgcgatgaa
     9781 cgtgggaata gtaccgacgc ctgaattaaa atccgtagac cagaaaagtg tttcgttgac
     9841 ttctacaggc ttgtttaaga gccatgtatt gacgaatcga tttggaccaa ctccaaaaat
     9901 aggatcttct ttgagtgccg ctttcacgat atgaagtgtg cttgaccaat tcggccgaac
     9961 ttcaagctgg gcaacatcga atgaatctga aagtctcata ccaaccccgt ttctacccaa
    10021 ggcaaaaata atcgctaaaa tcacgacagc caacgaaaca aagcttatct tttgtacgaa
    10081 ttttttacca ctcgtctcgc tttttggttt agcaactttc ctgaaccagg cgccgtaaat
    10141 aaacgatacg atggcgatca acccgatcac aatccaaact gcggtaaagt tgatgagggc
    10201 gagaaaaaag agagagacga tgagcgtcac ccacgagata aatttgacca aggggctaaa
    10261 cgacaaaaat tcgacggcga agagtcccat gattgcaccc gcgccgaaaa acaatcccac
    10321 atcgttccat ttaccaagtg tacttgctgc agcatttccg aaaagaccaa acgaaagaac
    10381 gtccggacca gcaaacaatc tgaccatgtg gtatagagca aaaacgataa gcgacgcgat
    10441 aaacgtagcg taaaaataaa aaattcgcgc ttcgtttttc actgtaagcg gaatgagaaa
    10501 caacagtgca aaagatatga tgactgctga gagcgttccg atttcgaacc cttggccgat
    10561 aaaagatcga gcaatggaat cagagaaaaa tgttgagagt acagtcgata cgattaccgc
    10621 aagtgctccc catgtcgtta tagtattgaa atacgatatt tttccttcct tgagtttcca
    10681 cgcactccaa acaataagca ttgttatgac tgtaattgcg atgaaaagac ttttgctaag
    10741 ttggaaggca accgatttca agaagaaaaa cggcagaaaa aaaacgaggg cgagaaaaat
    10801 ccagaggctt attttttcaa gaatacctac agacactctt tctgaagttt ccttttccat
    10861 tgatacgaat aattaaagtg taatagtcct tctaacggcc cgcgtatgac aaaccgcagt
    10921 tggcttatat tatatacaat ggcttttaac aatggaagag tttttaatag aaatttttga
    10981 gtacatttga gagacggttt tatcaagcct gaaggttgaa attgaagcca ttggtcttga
    11041 ttttttaaaa aaaaatgcta atataatcac agcttgtgtc gaatagctgc gccaattttg
    11101 tctgccatag gcggatacaa aattgagcgt cccgagtttg tcgagggact tgtccattcg
    11161 actcgcccgc ccaaggcggg ctcgctcagg acactcattt ttgcgccacc ttcggtgggc
    11221 aaaaatggtg aggaaagtcc gaacacagcc gagcaccact tttgttttga attaaggtta
    11281 aacctttttt tcgatccaag gttgaacctt ggaggcaata gtggtgctcg gagaacggta
    11341 gtgtcccgat tccgtttacg gaatcgagga tccacgattt ctatgcaagt agaaatcggg
    11401 aggtaattcc catccggaga aatccgcgag gtgcgaacag tgacgctcgg ttccgaaagg
    11461 atagagcccc ttcggggtga agcctggcgt aagccaggag tgcaacggct aaacccttac
    11521 ttctgtgcaa ggccgagcca ccggtgcacg gcatcggaat gactaatatc aaatatctaa
    11581 ttcataaata ttagaaatta agatttagaa attccgctgc cttgcagcgg tcggagtgcc
    11641 gctcgaggtg tgtggtaaca cacatcctag ataaatagct attgccgagc accacttttg
    11701 ttttgaatta aggttcaacc ttttttcgat ccaaggttga accttggagg caaaagtggt
    11761 gctcggtaca gaattcggct tatagataca agatatgcaa aaaccccgcc ctaaaggcgg
    11821 ggttttgctt tgtggtatat gccaagtttc aaagtaaaaa ttaaaaaaac cctcgagttt
    11881 atggcttgag ggttcgcgga gaggctaggt tcttattctc ttagatttgt cttacacgga
    11941 tcggccaaag cagatcggct actaacaaat cggaattatc cttttatgaa gagcggttgc
    12001 tcttcaacct tgtccgcatt agcgacgaga cgattaagtc ctgtccttga taggaatgag
    12061 ctcgtcgagc tcagccatgg tcttgtcgta ggcttccctg aacattagtt gcacaaactg
    12121 cgccgcttca tgaacaggaa tgccgagctt ccgagcctca tttgaaatgt cacgctttac
    12181 ttgtttcgga ttcaagcgga cgccctcttc ttccttccgg ctctgaaacg caaccattgc
    12241 aatttgacca actcgttctg acgtaagtgc cataagcact cctttcttct tttttgaact
    12301 aaataccttt acgtaaggca cgacggctga ttactactct tcatcaacta aaccattatc
    12361 tcaaacttat atgtatatgt caacaacccc gcatttgcgg tctcttttat aattactttg
    12421 aattacgaaa ctcccttttc ccacattttc atattaaaat acaaatggat gcctttaggc
    12481 atccatttgt atgaatactt ctatcaaacc gtacaactcc atactaacaa aagttacttt
    12541 tctcatcaa
//
LOCUS       DATDSU010000050         5576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2219081, whole genome shotgun sequence.
ACCESSION   DATDSU010000050 DATDSU010000000
VERSION     DATDSU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219081"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VI981_01670"
     CDS             complement(<1..673)
                     /locus_tag="VI981_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY4479047.1"
                     /translation="MTVFLFFVVLAVLVLVHELGHFLVAKWSGVRVDEFGLGFPPRLW
                     SKKIGETSYSINMIPFGGFVKIFGENPNDDSISGPDSTRSFVNKKRPIQLAVLVAGIV
                     FNVIFAWLLYSLVFMTSAPTIVTPENAARVRNAHVTVINVFSESPAQKAGLKPGDQIL
                     GVRTGADKIESTELDPETMGKFIAGHPEELTLDFKRGSSPTQTIAITAVSGIAKDQVA
                     IGISMD"
     gene            complement(768..1319)
                     /gene="frr"
                     /locus_tag="VI981_01675"
     CDS             complement(768..1319)
                     /gene="frr"
                     /locus_tag="VI981_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY4479048.1"
                     /translation="MAYNFSPFKDKLSSVEKWLVREMAGIRTGQATPLILDLIQVEAY
                     GSKVPVKEVASINVEDSRTLRIAPWDTSVIKDIERAIITSDLGLSVAVDESSLRVVFP
                     SLTAERRVSFIKLAKEKLEEAKISVRKEREKILKELDAKEDSGELNEDGVKKLRSEVQ
                     KLVDEINKKLDELRSRKEKEISA"
     gene            complement(1444..2082)
                     /locus_tag="VI981_01680"
     CDS             complement(1444..2082)
                     /locus_tag="VI981_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479049.1"
                     /translation="MELPEDIQNLHHAYCLISQETRTARDLITQLQSKYEMAFRGNPD
                     FIFIETDTVTIDIGRELKAFQSKKAFGPRKLIVISARSMTVDAQNSLLKTFEEPAAHT
                     HFFLILPRLSMLPTLRSRFSVIREIKNTTSTGNIRQFMGENRSGRLRIIKQILDRENK
                     KEVEVFLEDLEKYLHNTHTSAKKMSDVLKMKKYAFARSASLKQILEHLALVL"
     gene            complement(2084..3097)
                     /locus_tag="VI981_01685"
     CDS             complement(2084..3097)
                     /locus_tag="VI981_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY4479050.1"
                     /translation="MSFFSPKLGIDLGTANTLVYVPGRGVVLNEPSVVAVSAQDNKIL
                     AVGLEAKNMIGKTPESIIAYRPLKDGVIADYRVTEAMLRYYMSKALGKWNLFKPDVMV
                     SVPAGVTSTERRAVIEAAIKAGAKNAYVVKEPILAAIGAGIPIQEAMGHMIVDIGGGT
                     TDVAVISLGGIVASTSVKCAGNRIDAAIIDYIKKTFNLAVGDKMAEDIKIEIGSAVPV
                     EEELSMLIKGRDFITGLPRSAEIKTNEIVKAIGKELREMLKAIKDVLQETPPELSADI
                     IDMGIIMTGGSSQLRNLPELVFRRTGVKAVLAKDALYCVAKGTGIALQHLDTYKKTII
                     AKR"
     gene            3226..3915
                     /locus_tag="VI981_01690"
     CDS             3226..3915
                     /locus_tag="VI981_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479051.1"
                     /translation="MKRIEISARRQALLEVAAGLIVERGSSCLNVSELSARSGIPRPT
                     VYADFPHPNIRIRRMILVVFLRKLRESVETTLACLERNQSEVERIAAMFRAVIYTFKS
                     SGTIGRASTMLLLDQRLKRLPDLVWLQREAEVHLSRATARGELTCVAKAVGFPHIRSL
                     LFGVLFGLLQTLHVGPNPCPPRSKSDLPLTETEVHVEMLRILQVYCNDDAKSRLQSIM
                     DALLRKRAAAV"
     gene            4085..>5576
                     /locus_tag="VI981_01695"
     CDS             4085..>5576
                     /locus_tag="VI981_01695"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY4479052.1"
                     /translation="MGSKASQGMRDANSKLSQIGRLIQTLREERKITQNTFAKKLGTT
                     QSAIARIENGEQNLSTEMLSRISAALGRDVIQVSSGALNIEIEGGYRLSGTVTTKTSK
                     NGAVGLLCASLLNRGKTILKNVPRIEEVFRVIEVMESIGVGIRWRENDLEIIPPKKLS
                     LKKIDREAAIKTRSIIMFIGPLIHELSRFSIPQAGGCKLGSRTVRPHFFAMEKLGVSI
                     ETKASSYVITSPALKAGEVVLYESGDTVTENTLMAAARISGTTTIKFASANYQVQEIC
                     FFLEKLGVKIDGVGTTTLVVHGKKEIDESISYNLAEDPIESMFFIAAAAVTKSRITIK
                     RCPIDFLELELFKLEKMGLRFLIGKAYMSDNGKTRLVDIKIFPSKLVALDEKIEARPY
                     PGLNIDNLPFFAVIATQAVGTTFIHDWVYEKRAIYYKELDKLGADTLLADPHRTYITG
                     KTELRAAEVICPPALRPAAIILIGMLAAKGTSTLRNIYSINRGYEDI"
BASE COUNT         1453 a         1249 c         1284 g         1590 t
ORIGIN      
        1 tgtccatgct aattccgatt gcaacctgat cttttgcgat acccgaaact gcggtaatgg
       61 cgattgtttg tgtcggactc gagccgcgtt taaagtcgag cgtgagctcc tcgggatgcc
      121 ctgcaatgaa cttccccatt gtctctggat caagttccgt agattctatt ttatcggcgc
      181 ctgtacgtac accgagaatt tggtcacccg gcttgaggcc tgctttttgt gccggacttt
      241 ctgaaaacac attgatgaca gttacgtggg cgttgcgcac acgcgccgca ttttcggggg
      301 tgacaatcgt gggtgcgctt gtcatgaaga cgagagaata taaaagccag gcgaaaataa
      361 cattgaaaac aattcccgca acaagcacgg caagctgaat cggccttttt ttattgacga
      421 agctgcgggt gctgtcgggt ccactaatgg agtcgtcgtt gggattttcg ccgaaaattt
      481 ttacaaaacc cccaaatggg atcatgttga tcgaataact cgtttcccct atctttttac
      541 tccacagcct cggaggaaat cccaatccaa actcatccac acgaacaccc gaccattttg
      601 caactagaaa atgacctaac tcatgaacga gcactaaaac cgccaaaacg acaaaaaata
      661 agaatacagt catgaaaata taataccaat atacgaatgc gtaccaatga tactaataag
      721 ggaaattcat tgaatttgta taattggtga gaattagtat attagaatta tgcactgatt
      781 tccttttcct ttcttgacct aagttcatcg agttttttgt ttatttcgtc aaccaatttt
      841 tgaacttctg atcgaagttt cttcacgcca tcctcgttta gttctccgga atcttccttt
      901 gcgtcaagtt cttttaaaat tttctcgcgc tcctttcgta cggaaatttt tgcttcctcg
      961 agcttttctt ttgcgagctt gatgaatgag actcttcttt ccgcagtgag cgatggaaac
     1021 acgactcgga gtgacgattc atccactgcc acggagaggc cgaggtcaga agttatgatg
     1081 gcgcgttcaa tatcctttat gacacttgtg tcccacggag caatacgaag ggttcgtgaa
     1141 tcttcgacgt ttatacttgc aacttctttc acgggaactt tcgagccgta ggcttcgacc
     1201 tgtataaggt caagaatgag tggtgtcgcc tgccccgttc gaatacccgc catttcacgg
     1261 acgagccatt tttcaacgga tgagagtttg tctttaaacg ggctgaaatt gtacgccata
     1321 atggatgtga atacattcta tcgagatttt gcatctcggt aaaccccgtg ctcgctaaaa
     1381 tcaaattgtg ttctgcttct aaggggtaaa gattgctgtc gagccaaaaa agctgcaaaa
     1441 aatttagagc accagtgcga gatgctcgag aatctgcttc aaacttgccg agcgggcaaa
     1501 agcatatttc ttcattttca atacgtcact catctttttc gcggatgtat gcgtattatg
     1561 gagatatttc tccagatctt ctaaaaaaac ttcaacttct tttttatttt ctctgtcgag
     1621 gatctgcttt atgatgcgga gccgcccgct tctattttca cccatgaatt gacgtatgtt
     1681 tcctgtgctc gtcgtatttt tgatttctct tatgacgcta aatcgtgacc gaagtgtcgg
     1741 gagcattgaa agtcgcggta aaatgaggaa aaaatgagta tgagccgctg gctcctcgaa
     1801 ggttttgaga agcgagtttt gagcatcaac agtcatggaa cgggccgaaa tgacgataag
     1861 ttttctcggt ccgaaagctt tttttgattg aaaagccttc agctctcttc cgatgtcgat
     1921 cgttaccgtg tcggtttcaa taaaaatgaa atccgggttt cctcgaaatg ccatttcata
     1981 tttgctttga agttgtgtga tcaggtcgcg agcggtgcgc gtctcttggc ttatgagaca
     2041 atatgcgtga tgaagatttt gaatgtcttc aggtaattcc atattagcgc ttggcgatga
     2101 tggttttttt ataggtgtct aagtgctgaa gtgcaatacc cgtgcctttc gcgacacaat
     2161 aaagcgcgtc cttggccagt actgccttca ctccagtgcg cctaaaaact aactcgggaa
     2221 gattccgcag ttgcgatgaa ccgcccgtca tgataattcc catgtctata atgtctgcag
     2281 atagctccgg aggtgtttcc tgaagcacgt ctttaatggc ctttagcatt tctctaagtt
     2341 ccttgccgat ggcctttacg atttcgtttg ttttgatctc ggcactgcgc ggcaggcccg
     2401 taataaaatc gcgccctttg atcaacattg agagttcctc ctcgacggga acggcagaac
     2461 caatctcgat tttaatgtct tcagccattt tatcgccaac agcgagattg aatgtttttt
     2521 ttatgtaatc tattatggcg gcgtctattc tgttcccagc acactttacc gacgttgaag
     2581 cgacaatacc tccgagagaa ataactgcca catcagttgt tccgccgcca atatccacaa
     2641 tcatatgccc catcgcctct tgaataggaa ttccagcgcc gattgcggca agaattggct
     2701 ctttcacgac ataggcattc ttggcaccgg ccttaatggc cgcctcgatt actgccctcc
     2761 gttctgtcga agtcactccg gcgggcactg aaaccatgac atcaggttta aataggttcc
     2821 acttaccgag cgcttttgac atgtagtagc gaagcatcgc ctcggttacc cgataatccg
     2881 caatgactcc gtccttaagc ggtctgtagg caataatgct ttcgggagtt ttaccgatca
     2941 tattttttgc ttcaagacct actgcgagaa ttttgttatc ttgtgccgaa acagccacaa
     3001 cagagggttc gttcaaaacc actcctctgc ccgggacgta taccagtgta ttagcagtgc
     3061 cgaggtcaat tccaagttta ggtgaaaaga aggacataga agtatcgata gcgtgtagca
     3121 tatagcttat agcatgatgc gtaaagatgc acttgacaca tagtgtaaac atttcattgt
     3181 ttcgagcttt gacaacttct ctccatgtag tagtttgtag caagaatgaa gagaattgaa
     3241 atatcggcaa gaagacaagc gctacttgaa gttgcagcgg gcctcatcgt ggagcgcgga
     3301 agtagttgtc ttaatgtatc agagctgtct gctcggtcgg gtattccccg tccgacggtg
     3361 tatgcggatt tcccccatcc taatattcga attaggcgca tgattctcgt tgttttcttg
     3421 aggaaacttc gtgaatctgt cgagacgacg ctcgcatgcc tggaacgcaa tcaatcggaa
     3481 gtggagcgga tagcagccat gttccgcgcc gttatttata ccttcaaatc ctccggtacg
     3541 atcggacgtg caagcaccat gctacttctg gatcagaggc ttaagcgact tcccgacctc
     3601 gtttggcttc agagagaagc agaagtccat ctttcacggg cgacagcgag aggagaactt
     3661 acgtgcgttg ccaaggctgt gggctttcca catatacggt cgcttctttt tggtgttctg
     3721 ttcgggctct tgcaaacctt gcatgttggt ccaaatccat gccccccacg gagtaagagt
     3781 gaccttcctc tcacagagac cgaagttcac gttgaaatgc ttcgtatact gcaggtttat
     3841 tgcaatgatg acgcaaaatc aaggctccaa agcataatgg atgcacttct tcgtaaacgc
     3901 gcagccgcag tctaaatgcg gcttttttac tgtttgaata tatatctcat atatgctata
     3961 tatatgttta ccccgttaga agccgagatc acggatcgcg atctgcttct aataggatga
     4021 atgcaattcg gttttagcga acacggggtt taatgcccca tgtgagcttc taacggggtt
     4081 tattatgggt tcaaaagctt cacagggtat gcgggatgca aattcaaaac tttcccaaat
     4141 cggacggttg attcagacgt tgcgagaaga acggaaaata acccagaaca cctttgctaa
     4201 aaaactcgga actacacaaa gcgctatcgc ccgaatagaa aatggtgaac aaaatctctc
     4261 gaccgagatg ctttctcgaa tttcagccgc gctcggtaga gacgtcattc aagtgtcttc
     4321 aggggcgctt aacattgaaa tagagggtgg gtatcgtctt tctggtacgg ttacgactaa
     4381 aacttctaag aatggcgctg ttgggctttt atgcgcgtca ttgctgaata gagggaagac
     4441 cattctcaaa aatgttcctc gaatcgaaga ggtatttcgc gttattgaag ttatggaaag
     4501 tattggtgtc ggaatacggt ggagagaaaa tgatcttgag attattccac cgaagaagct
     4561 gtctcttaag aaaattgatc gagaagcggc cattaaaaca agaagcataa tcatgtttat
     4621 cgggccgctt atccacgaac tttctagatt tagcataccg caagccggag gttgcaaact
     4681 tggcagccgc acggtgcgcc cccatttttt tgcaatggag aagcttggtg taagcattga
     4741 aactaaagcc agctcgtatg ttattacatc gccagccttg aaagcgggcg aagtagtact
     4801 ctatgagtca ggcgatacgg taacggagaa tacactcatg gcagctgcta gaatttcggg
     4861 tacgaccacc attaaatttg catcggctaa ttaccaggtt caggaaatat gtttttttct
     4921 cgagaaactc ggcgtgaaga ttgacggtgt tggcacaacg acgctcgtcg ttcatggtaa
     4981 gaaggaaatt gatgaaagca tctcgtataa tcttgctgaa gaccccatag agtctatgtt
     5041 ttttatagcg gccgctgcgg ttacgaaatc gaggattaca atcaaacgct gccccattga
     5101 ttttctcgag ctggagcttt tcaagctgga gaaaatggga ttgcggtttt tgatcggtaa
     5161 ggcctacatg agtgataacg gaaaaaccag actggtcgat ataaaaattt ttccatccaa
     5221 gctcgtagct cttgacgaaa aaattgaggc tcgcccttat cccggactta atatcgataa
     5281 tttgccgttt tttgcagtga tcgcgacaca ggctgttggc acaacgttta ttcatgactg
     5341 ggtatatgaa aaaagggcga tttattataa agaactcgat aaattggggg ccgatactct
     5401 gcttgcagat ccgcatagga catatattac cggtaaaacg gaacttcggg ccgctgaagt
     5461 gatctgtccc ccggcgttgc gccctgcggc tattatcctc ataggaatgt tggcagcgaa
     5521 aggcacgtct actctgcgta atatttacag tattaaccgc ggatatgaag atatta
//
LOCUS       DATDSU010000051         4338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1221516, whole genome shotgun sequence.
ACCESSION   DATDSU010000051 DATDSU010000000
VERSION     DATDSU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221516"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VI981_01700"
     CDS             <1..235
                     /locus_tag="VI981_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479053.1"
                     /translation="FERCGSAAFPDFRGEIFSEKWVRTNSRILHQTGKVKEFWFASLR
                     LASNLYSILGVKTNWRFRILGEENFLILNPKTI"
     gene            complement(1687..1761)
                     /locus_tag="VI981_01705"
     tRNA            complement(1687..1761)
                     /locus_tag="VI981_01705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1726..1728),aa:Arg,seq:cct)
     gene            2483..3505
                     /locus_tag="VI981_01710"
     CDS             2483..3505
                     /locus_tag="VI981_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY4479054.1"
                     /translation="MTYEEEVMQAVEHILPSVVSIVISRHAHLVGPTLPFKGVEQQNA
                     QTGEGLDVKIGGGSGFIVSDDGLVLTNKHVIMEPDANYTIFDQNGDKSDVQVLARDPI
                     HDVAILKMIPPKHFRIAPLGNSHTLRLGQTVIAIGNALGEFRSSVSTGVVSGLSRLIT
                     AVSDLSGQQERLRGLIQTDAAINPGNSGGPLINTRGEVIGINAAVVFGAQNIGFAIPI
                     EKATKDLEDIKQFGRIRRPFIGIRYLIINAELQKQLRLPLDYGALVVREPIPGDHAVV
                     PGSPAEKAGIKEGDLVIACNNHAITEKNSLEDYLENTKPGGKISLTVLRNGEKHSLAL
                     ELKEWK"
     gene            complement(3638..3970)
                     /locus_tag="VI981_01715"
     CDS             complement(3638..3970)
                     /locus_tag="VI981_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479055.1"
                     /translation="MEGMEGFEKDASPLFEEEEEPEPRGIELYLGRVKSDRARGFVHG
                     FFGTVDRLTFEIDPKKREALEVDQQEFIDGLREEGEDEVANEFENMVGALIDTADAPI
                     EREDDGES"
BASE COUNT         1240 a         1017 c          939 g         1142 t
ORIGIN      
        1 ttttgagcgg tgcggctccg ccgcctttcc ggattttcgc ggggagattt tttcggaaaa
       61 atgggttcgg actaattcaa gaatactgca ccagacagga aaagtcaaag agttttggtt
      121 cgcctcgctt cgcttggcga gtaatttgta ttcaattttg ggggtaaaaa cgaattggcg
      181 atttcgcatt ttgggggaag aaaatttttt aatccttaat ccaaaaacaa tatgaaatat
      241 atactttatg caagaaaatc taccgaagat gatgaccgac aggttttaag tattgaagcg
      301 cagctcgtag agttgcaaga gtatgccgcc aaagaaaaac ttgaaattgt cgcttccttt
      361 caggaagcca aaaccgcaaa agaacctggg cgaattaaat tcgcagaaat gttgtcattg
      421 attgagagag gaaaagcaga tggcattatt tcttggcacc ccgaccgcct tgcacgaaat
      481 agtgttgatg gtggcaagat aatccacttc gtagatcgcg gcttgattcg ttcccttaaa
      541 tttcccacct tttggtttga gccaacgcca cagggtttgt ttatgctcaa tatcgctttc
      601 ggacaatcca aatattttgt agataattta cgagagaatg tgaagcgagg tttgcggcaa
      661 aagatacgaa atggcgtatg gcccggctgg gctccggtgg gctatctaaa caacaccaag
      721 acgcgctgta ttgacgttga tagcgagaaa gcccctaaag ttcgaaaaat gtttgaaatg
      781 tacgtcaccg gagcatatac gcttcactct ctcgcaaatt ggtgcaaaga acacgactta
      841 cgaggcaatc tcggcaaaga gatagcattg agtaatgtgc aaagtatcct acaaaatatt
      901 ttctacatcg gtctcatgaa atatggtggg gaaattcatg aaggacaaca cgagccgtta
      961 atttcaaaga aactttttga tacctgccaa gaagtgatga gcaagcgcgg gaaatttcac
     1021 catgtgagga aaaatgattt tgcttttctt ggcctcttaa aatgcgcttc atgcggttgc
     1081 tcgattactg ccgaaaaaca aaaaggtcat aactactatc gctgtactcg caagaaaggg
     1141 ccgtgccaag aaaagcatta tttgcgcgaa gaagcattga ccgagcaaat cacttcttat
     1201 cttcaaaaag tttctttgtc gagccaagac accgagaaag ttttggcgac gttagacagc
     1261 gaacaggata aagcgcgaga ggacgcgcaa aatgaagtta gcgttttgaa agagcagtta
     1321 gcacaagttg agacgaaact tcaaaaacta ttagacatct atttagtaga cgctttatcc
     1381 acagaggaat acgccgccaa aaagcaaagt ttgctttccc aaaagttcgc gcttgcggag
     1441 aagatcactg actttgagac aaaaggattg tcttggctcg aaccggcttg tgagttcgta
     1501 aaatccttaa atcaagccgc taatctgctt tcctctccca atcctactgc aatgacaaca
     1561 tttctgaaaa acatcggctc gaaccatatt ttgcggaatt gcgaattcgt ttttaccccc
     1621 aaaattgaat accaattagt cgccgagcga agcgaggcga accaaaactc tttgactttt
     1681 cctttctggt gcccctgcga ggaattgaac ctcgatttag agcttaggag tctctcgttc
     1741 tatccgttga actacagagg cagttaaact atctacttga tttataatag aaaagatttc
     1801 ttttaacaac aaaccgcccc gccttactcg aattctgcac atgccatatt ttaactttcc
     1861 tctctttcga ccttgtagga tgttaatact cgtggatctg ccaagcttta taccagtaat
     1921 ggcagaccaa aactttaaac actccagttg atctaagtct tcataaattc ttaaagaaat
     1981 ctttaaatct ttatctttta ccttaaaggc attccgtaga ataaagagaa atattcgtat
     2041 caatactgga tcggtatttg aaagactgaa atcttttttt gaaccttccg cccaatataa
     2101 agcggcacca gtcaatatta aatctttctc agtgattgat cgaaccgcct ttcgcgcacg
     2161 agctcgagct tcttgccagt ggcgttccga aattatctta ctcgcattcc gacgcgcgcg
     2221 ccatcggcct tgatattgag gtagtatgga tatgtttttt acgtagcgcg ctgcagtgga
     2281 ctttgaaatc ttacacgccg cagtaatttc agggatacta tgacctcgtt ctcgcaaatg
     2341 tataatctgt ttgatcagca ttccggataa agacatcgat ttaataatat cgaaactatc
     2401 aagttcgttc aagaacgatc tagatttatc cacaacactc gacgattcta aaaacacata
     2461 atttattgat atactagaag ctatgacata cgaagaagag gtcatgcagg ctgtggagca
     2521 tatcctgcca tcggttgtat ccattgtcat atcgagacat gcccatctcg tcggacccac
     2581 cctccccttc aaaggggtgg aacaacaaaa cgcccagaca ggagaaggtc tcgacgttaa
     2641 aatcggtggc gggtcaggct ttatcgtatc agatgacggg ctcgtcctca ccaacaaaca
     2701 cgttatcatg gaaccggatg caaattatac tatctttgac cagaacgggg ataaaagcga
     2761 tgtccaggtt cttgcgcgag atcccatcca cgatgttgca attctcaaga tgataccgcc
     2821 gaaacatttt cgtatagcac cgctcggcaa ctcgcacact ttgcggcttg ggcagacggt
     2881 tattgccatc gggaacgcgc tcggagaatt tcggagttcg gtctcgacgg gtgtcgtctc
     2941 gggtctctcg cgcctaatca cggcggtcag tgatctctcg gggcaacaag agcgtctacg
     3001 aggactcatc caaacggacg ccgcgattaa tccaggaaac tccggtggtc cgcttatcaa
     3061 caccagaggc gaggttatag gcatcaacgc ggctgttgta ttcggtgcac aaaatatcgg
     3121 atttgcaatt ccgattgaaa aagcgacaaa agatctcgaa gacatcaaac aattcggacg
     3181 catccgccgc ccatttatcg ggattcggta cttaatcatc aacgcggagc ttcaaaagca
     3241 acttcgcctg ccactcgact acggagcact cgtggtgcgt gagccgattc cgggcgacca
     3301 cgcggttgtc cccggaagcc ccgccgaaaa agccggtatt aaagaggggg acctcgtgat
     3361 tgcgtgcaat aatcacgcca tcacggaaaa aaattcactt gaggattatc ttgaaaacac
     3421 caaacctgga ggaaaaatat cactcaccgt ccttcggaac ggagagaaac actcgctcgc
     3481 gctcgagctg aaagagtgga agtaaatccc gttataaatt cctgcggtgt attttaagcc
     3541 atcgcgcgaa ttgtcttaaa tagagagcag cggggtatta tttctaacgt gataaaaaat
     3601 agttctttgg aactaaatta aggtttttgg atacgctcta gctctcgccg tcgtcctctc
     3661 gctctatagg cgcatcggca gtatcaatca aagcgccaac catattctca aattcatttg
     3721 caacttcatc ttcaccctcc tctcggagac catctataaa ttcctgctga tcaacctcga
     3781 gtgcctctcg ttttttgggg tcaatttcaa atgtgagtct atcaaccgtg ccaaaaaaac
     3841 catgtacgaa tccgcgcgcg cggtcactct tcacgcgccc taagtatagt tcaataccgc
     3901 gcggctccgg ctcttcctct tcttcaaaaa gaggacttgc gtctttttca aaaccttcca
     3961 tgccttccat atatatattt tacctttctt tgcaggcgcc cccctgtgca tactcactag
     4021 agcctatctc aaaaacccaa tttctgctgc aatgacaggg tttttggctg caagccgttc
     4081 atccctactc gaaatagcaa tactattcct cgtcgggctt catggctttc gctcaaaagt
     4141 cccgcatttc gctacgaaag cgatttttga gataggctct actcaacgca cccgccaaat
     4201 gtgaaagtat ataaacgaat tccggcctcg cgcggaatga gcttgaggaa cgcagaaccc
     4261 gcatcttttc gctttacaag attcaacatc tgcggatgag aaacggtgag cggcggaagt
     4321 tttcgcccgt caagcaaa
//
LOCUS       DATDSU010000052         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1222890, whole genome shotgun sequence.
ACCESSION   DATDSU010000052 DATDSU010000000
VERSION     DATDSU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222890"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VI981_01720"
     CDS             <1..436
                     /locus_tag="VI981_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY4479056.1"
                     /translation="EPIIAKKLFDKVQEVLKQRGRPRHKSKIEPQVFCGLLRCDTCGM
                     MITGEYRVKKQKNGNEHYYTYYHCTKKRKGFKCGEPCIRQEELDKQISSLLQKVSLRP
                     DWAKKLQIRLEKDKTKSAQSVSAFVQESQEKIQIIQKAPATS"
     gene            complement(772..847)
                     /locus_tag="VI981_01725"
     tRNA            complement(772..847)
                     /locus_tag="VI981_01725"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(812..814),aa:Thr,seq:ggt)
     gene            complement(1063..1683)
                     /locus_tag="VI981_01730"
     CDS             complement(1063..1683)
                     /locus_tag="VI981_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4479057.1"
                     /translation="MQTLALLARSRWVNFSMNILLASAWSVFVWNHLRSFNITGDYAL
                     IAFALSETLQVLFFLIRRSPLSVSLDPFDWLVAGGGTLAPLFLIPGGPVLWQSGEILV
                     IIAVVFQIFALLSLNRSFAIVAAKRVVKTSGLYRIVRHPMYASYLLLYSGYLLFNGSL
                     MNIGLVVLAFVFLFLRIIEEEKHLIVDQEYEQYATRTPWRLIPFVY"
     gene            1917..1987
                     /locus_tag="VI981_01735"
     tRNA            1917..1987
                     /locus_tag="VI981_01735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1949..1951,aa:Met,seq:cat)
     gene            2154..2336
                     /locus_tag="VI981_01740"
     CDS             2154..2336
                     /locus_tag="VI981_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479058.1"
                     /translation="MPTGRRLGVFWLTPIKFLYQEKFKTKGEAARREKQIKGWRRKKK
                     FNLIKFGKLIISPVVF"
     gene            complement(2337..>2599)
                     /locus_tag="VI981_01745"
     CDS             complement(2337..>2599)
                     /locus_tag="VI981_01745"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY4479059.1"
                     /translation="EFGKWFVEQIQKDHRYTRVGKPNDNAHVERVNRTLQEECLDKTV
                     RTPEAFNRALRKYLPYYNTKRLHLGISLRAPQELLTKVIPGY"
BASE COUNT          811 a          581 c          602 g          605 t
ORIGIN      
        1 cgagccaatc atcgcaaaga aactttttga taaagttcag gaagttttga agcagagagg
       61 aaggccgcgc cacaaatcca aaattgaacc gcaagttttc tgcggattat tgcgttgcga
      121 cacttgcggt atgatgataa ccggcgaata cagggttaaa aaacaaaaga acggcaacga
      181 gcattattac acttattatc actgcacaaa aaagcgaaaa ggttttaagt gcggcgagcc
      241 ctgtattcgc caagaggaat tggacaagca aatttcctct ctgcttcaaa aagtttcttt
      301 gcgaccggat tgggcgaaaa aattacaaat aagattagaa aaagacaaaa caaaatccgc
      361 ccaatccgtt tctgcttttg ttcaagaatc gcaagaaaaa atccaaatca tacaaaaagc
      421 tccagcgact tcttgacggc tatttagagc aagacataga ccgagaaata tatcgcatag
      481 aaaaagcaaa actattatat gaaaagaagt cgctggacga gcagaatatc aaacttgaac
      541 aaaagcagaa tgattggctc gaaccgatgg aaaaatggat aaaagtcgct tcaaatcttg
      601 aaaaaaacgc aagggataac aacctttttg agaaaaaggt tgttgccgaa caagtgttcg
      661 gctcgaacct cactctaaaa acgagcgaag cgagcggaat tggcttggga caatgggcaa
      721 agctccgctt tgcccgtcaa aattttagaa aaactgacct cactttactt atggagccga
      781 tgcccggaat tgaaccgggg acctactcct taccatggag ttgctctacc agctgagcta
      841 catcggcgaa tgagggataa aatgggaact cgttcacatt tttcccgaat gatggcgacg
      901 tatattttat acatcggcaa acgaggaaca aaattaagaa tatctcatat gcttaatttt
      961 tccgagtgct gccgttgcat gaaatataca acagcaacca tattcttact cttacagaat
     1021 ggcgttgctt atcgtaccct attacttatt ttttttcaat tcttaataga caaacgggat
     1081 gagccgccac ggtgtccggg ttgcgtactg ctcatactcc tgatcgacga taagatgttt
     1141 ttcctcttcg ataatgcgca aaaaaaggaa aacgaaagca agcacgacga ggccgatgtt
     1201 catgagtgac ccgttaaaga ggaggtatcc gctgtacagc agtaaataac tcgcatacat
     1261 cgggtggcga acgatacggt aaagtcccga agttttcacc acgcgttttg ccgcaacgat
     1321 cgcaaaactc ctattgagtg acaaaagggc gaatatctga aaaaccaccg caatgatcac
     1381 caagatctca ccggactgcc acaacaccgg gccgccggga atcaaaaaaa gcggagcgag
     1441 cgttcctccg cccgccacaa gccagtcgaa cggatcaagc gataccgaga gcgggcttcg
     1501 gcgaatgaga aaaaatagca cttgcagtgt ttcggaaagt gcgaaagcga tgagcgcata
     1561 atctccggtg atattgaatg accttaggtg attccataca aacacactcc atgcggaggc
     1621 gagaaggata ttcattgaaa aatttaccca ccgtgatcgg gcaaggagcg ccagcgtctg
     1681 catgttaccc ctatcgtatc atattgtcct tgctctcggg cttgattttt tgacgtttat
     1741 tcgctacact ctgagcacat tagcaaggta tggatatgac ggatgaactc agtgtgattt
     1801 cgcctgaaag acaacggaag aaaatatata gcagggtgag ggaagacagc acctcaagtc
     1861 atgacagcct catgagcctg aaggaaatga gcaagagtgg gaaggtaaaa atatatagcg
     1921 ggatggagga gcggtacctc gtcaggctca taacctgaag gccctggttc gattccaggt
     1981 cccgcaacac ttcgacaagc tcagtgcaaa ctgagattaa aaagcgccct gaggacattc
     2041 ggctgagctc agtcgaagcc tttacgaggg gcgcttttta attagggcgg aagatatacc
     2101 aagctccaca agggagtaaa acatgccgcc cgtctatccc cgcccttacc tacctgccga
     2161 caggcaggcg tctcggggta ttctggctca caccaataaa atttctttac caagagaaat
     2221 tcaaaaccaa aggagaggcc gctcgtcggg aaaaacaaat caaggggtgg aggaggaaga
     2281 aaaagttcaa cttgataaaa tttggtaagc tgataatttc accagtcgta ttctaatcaa
     2341 tagcctggaa tcacttttgt taataattct tgtggcgcgc gcagggaaat tcccaaatga
     2401 agccgttttg tgttgtagta cggaaggtat ttcctgagcg cccgattgaa cgcttccggt
     2461 gtacgcacgg tcttgtctag acattcctct tggagggtgc gattcactcg ctccacatgc
     2521 gcattgtcgt tcggctttcc aacgcgcgta tatcgatgat ctttttgtat ttgttcaacg
     2581 aaccacttcc caaactccg
//
LOCUS       DATDSU010000053         5437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1394155, whole genome shotgun sequence.
ACCESSION   DATDSU010000053 DATDSU010000000
VERSION     DATDSU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394155"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1213
                     /gene="pgk"
                     /locus_tag="VI981_01750"
     CDS             86..1213
                     /gene="pgk"
                     /locus_tag="VI981_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY4479060.1"
                     /translation="MNLKTIYDIKDLAGKRILMRVDFNVPLEAGKVGEDYRLRKIFPT
                     INYLRDAKAKIILISHIENVKDNSLRPVFDYLSYHFPVTFLTEPIDEVKSKLALQKEG
                     EVVLLENLRKNPGEKENDDVFAAKLASFGDLYVNEAFPVSHRAHASIVGVPKFIPAYA
                     GIRFVQEIKELSKSFTPPKPFLFIIGGAKFETKMPLIKRFLDSADHVFIGGALANDCF
                     KAKGWPVGNSVVSNSSLDLTPIINHPKVILPKDVVVEKGKELIEKKPSEVAEGEKILD
                     AGPESLNDLRLLIQKSLFVLWNGPLGNFEAGFKEATLELAQIIANTETTSVIGGGDTV
                     GAISELRLEQKFSFISTGGGAMLEYLANGTLPGIQALENSK"
     gene            complement(1214..1588)
                     /locus_tag="VI981_01755"
     CDS             complement(1214..1588)
                     /locus_tag="VI981_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY4479061.1"
                     /translation="MDQKDCIFCKIVANEIPSEKVYEDENFLAFLDIHPQSPGHALVI
                     PKTHYRWVWDMPNAGDYFEVAKKIARAQQKAFATEWILSKIIGDEVPHAHIWVFPDPK
                     IPGDRADLKVNCQKIKAALTTI"
     gene            1639..2322
                     /locus_tag="VI981_01760"
     CDS             1639..2322
                     /locus_tag="VI981_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY4479062.1"
                     /translation="MKSYVVRSQPPAYVCESLSAYPPLLQELLYSRGIDNPKEADIFL
                     NPSYDDHVHDPYLMKDMDKAVERILKAIHNDEHVVIYSDYDADGIPGAVVLHDFFKKI
                     GFKNFENYFPHRYDEGYGLNENAVLSFSKSKTTLLVTIDCGISDLVSVARANELGIDV
                     VITDHHLSQEGKVPKAFAIVNPKQEECGYPCDMLCGSGVIYKLIQAILVKKNFGIKKG
                     AEKWFLDMV"
     gene            2332..3354
                     /locus_tag="VI981_01765"
     CDS             2332..3354
                     /locus_tag="VI981_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY4479063.1"
                     /translation="MADMVPLLGENRIFAHYGLRVLRKSPRLGLMKLLRYANVDQRFL
                     NEDDVGFTIAPRINAASRMGIPREAFDLLTAVEDTKADRLSLYLQQINEERKGMSAFL
                     VKEVRKIVESRKEKGEPTEIIVVGNPNWKPALLGPVANNILDAYGVPVFLWGRADGDV
                     IKGSVRSDGVVNVLELMKHALPDTFIGYGGHKCSGGFSVSHTNIHQLEANLKEAYDKL
                     PKIKISDSFLTVDRRLTLEEVTWDTYRIVEMMSPFGEGNPKPLFVFDNVAPSIVKMFG
                     KEKNHLEITFTNTRNKVKAIRFFFKSKSENVVPEVGKRLSFVAAMEKSYFRNYPELRL
                     RIVDIL"
     gene            3351..4118
                     /locus_tag="VI981_01770"
     CDS             3351..4118
                     /locus_tag="VI981_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEY4479064.1"
                     /translation="MNEKVGYIFTDGSSRGNPGPGGWGAIVVFDFQFSTFSDEVKVTE
                     LGGYEDRTTNNRMELTAVIKALEFLNASAEGGSASGGKSYKLFSDSRYVINGASKWVH
                     GWKKNNWITSSKNPVQNKDLWEELDYLAQTAALNWVYVGGHVGIAANERCDQIATAFA
                     DGKKVELYSGILANYALKNILDMSTDSAKEVSKSSTRARGRAKAFSYVSKVHGKIETH
                     KTWADCEARIKGQSGALYRKTLSKEEEKSLINLWRNA"
     gene            4111..>5437
                     /locus_tag="VI981_01775"
     CDS             4111..>5437
                     /locus_tag="VI981_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY4479065.1"
                     /translation="MHKLRDKIFFSTILGFTLGIFVRSFVEIDFVLVLCALVVVFGLL
                     GISLTTKKPLIGLFALALLAGNVGVLRYSQKDVKPNEILVSLEGQQATLKGEIVRDPD
                     RREKSAFITVALDEVNDYTFEKGKPLVRIRTELFPQLFYKDSVAVEGKITRPESFLTD
                     SGREFDYASYLGKDEIYFEVSFAKLITYEHEPSLVGLLYATKQGLEHEMKKVVPEPKA
                     SLLAGILLGSKKGLGSELEEKFRKTGVIHMVVLSGYNITVVAEFLQKIFSFLPKMFGL
                     ASGALGIIIFAILTGAQATVIRASIMALLVLFARGTGRTYNVTRALVFAGFLMILHNP
                     KILVYDYSFQLSFLATIGLIYFSPLVERKLMWIRPPKLRDIVVSTVATQFFVLPMILY
                     RIGDLSTVALLVNILVLAFVPAAMLLGFVSTMASFISFSVALPFAYITAL"
BASE COUNT         1508 a         1148 c         1294 g         1487 t
ORIGIN      
        1 ctttaataca agaagatgat attaccaatt aattacgccc cctcccatcg gtgtgtcagg
       61 cgatggcagg cgggcctcta acgggatgaa ccttaaaaca atttacgata taaaagacct
      121 cgcagggaaa cggatactca tgagagtcga tttcaatgtt ccgcttgaag cagggaaagt
      181 tggtgaggac taccgtttga gaaaaatatt tccgactatt aattatcttc gggatgcgaa
      241 agcaaaaatc attcttatct cgcatattga gaatgttaaa gataattcac tgcggccagt
      301 gtttgattat ttaagttacc attttccagt cacttttttg acggaaccta tcgatgaagt
      361 gaaaagcaag ctggcattgc agaaggaagg tgaagtggtc ctgctcgaaa atttaagaaa
      421 aaacccgggt gagaaagaaa atgatgacgt ttttgccgct aagctggcct cgtttggcga
      481 cctctatgtg aacgaagcat ttcccgtctc gcatcgggct catgcctcaa tcgttggagt
      541 gcccaagttt atccctgcat atgctggtat ccgcttcgtg caggaaataa aggaactttc
      601 aaagtcattt acaccgccta aaccctttct ttttattatc ggcggagcaa agttcgaaac
      661 aaagatgcca ctcattaaac gctttcttga ttcagcagat cacgttttta tagggggggc
      721 actcgctaat gactgtttta aggcaaaagg atggccagtc ggcaattcag tcgtgtcaaa
      781 ttcttcgctc gatcttaccc caatcattaa tcatccaaaa gttatattac cgaaggatgt
      841 cgtagtggaa aaaggaaaag aacttatcga gaaaaaaccg agcgaagtcg ctgaaggaga
      901 gaaaattctc gacgctggac ctgaatcatt gaacgacctg cggcttttga ttcaaaaaag
      961 tttatttgtg ctatggaatg gtccactcgg taattttgaa gccggattca aggaggcgac
     1021 gctcgagctt gcccaaatta tcgcaaacac cgaaacaacc tcagtcatag gcggcggtga
     1081 cacggtggga gcgatttctg aactccgttt ggaacaaaaa ttcagtttta tttcaacggg
     1141 cggtggagcg atgctcgaat atcttgccaa tggaacactg cccggaatac aagcgcttga
     1201 aaattcaaaa taattaaatt gttgtaagtg cggctttgat cttttgacag tttactttta
     1261 agtcagcgcg gtcgcccgga atttttgggt cgggaaaaac ccaaatatga gcatgaggca
     1321 cttcgtcgcc tataattttg ctcaagatcc actcggtggc gaacgctttt tgttgcgcac
     1381 gcgcaatttt ttttgccact tcaaaataat ctcctgcatt tggcatatcc catacccacc
     1441 ggtaatgggt tttagggatg acgagcgcgt gcccgggtga ctgcgggtgg atatcaagaa
     1501 aggcaagaaa attctcatcc tcatacactt tttccgatgg aatctcgttt gcaacaatct
     1561 tgcaaaatat gcaatccttt tggtccatag caacagtata tcaaaaaagt ggcgtccctt
     1621 ccgtgttaaa atagtcatat gaagtcttat gttgtacgat cgcaaccgcc ggcgtatgtg
     1681 tgcgagagtt tgagtgcata tcctccgctc cttcaggagc ttctttattc aagggggatc
     1741 gacaatccga aagaggcgga cattttttta aatccgagtt atgacgacca tgttcatgat
     1801 ccgtatctca tgaaagatat ggacaaggca gtggagcgaa tactaaaggc cattcacaat
     1861 gatgaacatg tagtgattta cagtgattat gatgctgatg gtattccggg agcggtagtc
     1921 ttgcacgact tttttaaaaa aatcggattc aaaaattttg aaaattattt tcctcaccgc
     1981 tacgatgaag ggtatggcct gaatgagaat gctgtcctgt ctttctcaaa aagcaagacc
     2041 acgttactcg ttactataga ttgcggcatt tctgatctcg tatcagttgc tcgtgcaaat
     2101 gagctcggta tcgacgttgt tattacggat catcatctct cgcaggaagg aaaagttccg
     2161 aaggcatttg ccattgtcaa tccgaagcag gaggagtgcg gatacccatg cgacatgctc
     2221 tgcggatcag gcgtgatcta caagcttatt caggctattc tcgtcaaaaa gaatttcgga
     2281 ataaaaaagg gtgctgaaaa atggttcctc gatatggtct gattggcgac catggcagat
     2341 atggtgccgc ttcttggcga gaatcgaatt ttcgcacatt acggtctgcg agtgcttaga
     2401 aaatctccac ggttgggact catgaaatta cttcgatatg ccaacgtcga tcaacgtttt
     2461 ctcaatgaag atgacgtcgg ctttacaatc gccccccgca tcaatgccgc gtcccgaatg
     2521 ggaattccgc gcgaggcctt tgacctctta actgcagttg aggacacgaa agcagaccga
     2581 ctttctttgt atctgcagca aattaatgaa gagcgaaaag gcatgagcgc ctttcttgta
     2641 aaggaggttc ggaaaatagt tgaaagccgg aaagaaaaag gggagcccac cgaaattatt
     2701 gtagtcggca accccaattg gaaaccggcg cttcttggcc ccgttgcaaa caatatcctt
     2761 gatgcgtatg gcgtgcccgt ttttctgtgg ggcagagcag atggtgacgt catcaagggg
     2821 tcggtacgaa gcgacggtgt tgtgaatgtt cttgagctta tgaaacacgc tctgcccgat
     2881 acgttcatcg gatacggcgg acataaatgt tccgggggat tttcagtgtc tcacacaaat
     2941 attcaccagt tggaggcgaa tctaaaagag gcgtacgata aattgccaaa gataaaaata
     3001 agcgactctt tccttaccgt tgacagacgt ctgactctcg aggaagtgac ctgggacaca
     3061 taccgtatcg ttgagatgat gtcgccattt ggcgaaggta atccaaaacc actttttgtt
     3121 ttcgacaacg tagcaccttc gattgttaaa atgttcggaa aagaaaaaaa tcatcttgaa
     3181 attactttta cgaatacaag aaataaagta aaggccatac gtttcttttt taaaagtaaa
     3241 tccgaaaatg tagttcccga agtcggaaag aggctctcgt ttgtcgcagc aatggaaaaa
     3301 tcttatttca gaaattatcc cgaattgcga cttcgaattg tggatatttt atgaacgaaa
     3361 aagtcggata tatttttaca gatggatcat cacgcggaaa tccgggccca ggaggttggg
     3421 gagcgattgt ggtttttgat tttcaatttt cgacattcag tgacgaggtg aaagtcactg
     3481 aacttggagg atacgaagat cgaacaacga ataacagaat ggaacttacg gctgtcatca
     3541 aagctctcga atttctaaac gcttccgccg aaggcggatc cgcctcgggc ggaaaaagct
     3601 ataaactatt ctctgactcc cgctacgtca tcaacggcgc atcgaaatgg gttcacggtt
     3661 ggaagaaaaa taactggatt acgtcgtcaa aaaaccctgt gcagaataaa gatttatggg
     3721 aagaactcga ctaccttgct caaactgcgg ctctcaattg ggtctacgtg ggaggacacg
     3781 ttggcattgc ggccaacgag cgctgtgacc aaattgcgac agctttcgcg gatgggaaaa
     3841 aagttgagct ctattccgga atacttgcaa attatgcttt aaaaaatatt ttagacatgt
     3901 cgaccgattc cgcaaaagaa gtttcaaaat caagcacccg ggcgcgagga agggccaaag
     3961 ctttttcgta tgtcagcaag gttcacggaa agattgagac acataaaacg tgggccgact
     4021 gcgaggcaag aataaaaggg cagtctggcg cgctctatcg gaaaacgtta tcgaaggagg
     4081 aagaaaaatc gcttataaat ctgtggcgca atgcataaac tgcgtgataa aatatttttt
     4141 tcaactattc tcggttttac gctcggtatt tttgtccgct cctttgttga gatagatttt
     4201 gtactggtcc tttgcgctct tgttgtcgtg ttcgggcttc tcggtatttc actcacaaca
     4261 aaaaaaccgc tgatcggact ttttgcgctt gcccttttgg ccggaaatgt gggagtttta
     4321 cgctacagcc agaaagatgt aaaaccgaat gaaatattgg tcagtcttga gggacagcag
     4381 gcaactttga aaggggagat tgtgcgcgat cctgatcggc gagaaaaatc ggcttttatt
     4441 acggttgcac tcgacgaggt aaatgactac acgtttgaga aagggaagcc gcttgtccgc
     4501 attcgcacag aacttttccc gcagctgttt tataaggact ctgttgcggt ggaaggaaag
     4561 attacaaggc ctgaaagttt tctgacagac agcggtaggg aatttgatta tgcatcatat
     4621 cttgggaaag atgagatata ttttgaagtc tcattcgcga aactgatcac ttatgagcac
     4681 gaaccgagtt tggtaggact cttgtatgcg acgaaacagg gtttagagca cgaaatgaaa
     4741 aaagtggtac cggagccaaa agcatctttg ctcgcgggca tcttgctcgg ttcaaagaag
     4801 ggtctaggta gtgagctcga ggagaaattt agaaaaacag gggtgataca catggtcgtg
     4861 ctttccggat ataacataac cgttgtcgcg gaatttctgc aaaaaatatt ctcgttcctg
     4921 ccgaaaatgt tcggtcttgc ctctggagcg ctcggcatta tcatctttgc cattctcacg
     4981 ggtgctcaag cgacggttat ccgcgcgtca atcatggcgc ttctcgtttt gtttgctcgt
     5041 ggtacaggcc gtacatataa cgtcactcgc gcgcttgtgt tcgcggggtt cctgatgatt
     5101 ctccacaatc cgaaaatcct cgtatacgat tattcttttc agctctcgtt tcttgctact
     5161 attggtctta tttatttctc gccacttgtt gagcgcaaac tcatgtggat acgaccgccg
     5221 aaactgcgcg atatagtcgt gtcaactgtt gccacacaat tttttgtttt accgatgatt
     5281 ctttatcgaa ttggcgatct ctcgaccgtg gcgcttctcg taaacatact agttcttgca
     5341 tttgttcccg ccgccatgtt gctcggcttt gtcagtacta tggcaagctt cataagcttc
     5401 agcgttgcgc tccctttcgc ctacatcaca gctctga
//
LOCUS       DATDSU010000054         7755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_557602, whole genome shotgun sequence.
ACCESSION   DATDSU010000054 DATDSU010000000
VERSION     DATDSU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557602"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VI981_01780"
     CDS             complement(<1..766)
                     /locus_tag="VI981_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY4479066.1"
                     /translation="MPHIPKSALAGEVEIGLRLLGRGKVRNQYALPDGHEDKFLSLTT
                     NRVSAFDFVLPDKIEYKGPSLSAIHHYWNGFLSPYFKTDNVALGADVDRYLPAEIRGR
                     PDIQAVAKVAKDLSGTDTEEIFRFLLLGSGWSDYQRDSGVVCGHKLPDGLREGDELPI
                     PLYAPSTKEKEGHDKNIHHALVEKRFPGRSKLVLEIAQVARHHAMTKDILIADGKLEI
                     FGDVLGDEIFTPDSCRFFGAEAYRRARAKGALPPSLD"
     gene            1070..2122
                     /locus_tag="VI981_01785"
     CDS             1070..2122
                     /locus_tag="VI981_01785"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4479067.1"
                     /translation="MPREEAYLGSTNAMKISLLIPCYNEEKTLHASIQSWLLQTRRPD
                     QILVIDDSSTDNSSRILASYAPYIETRRTPQNLGNKSYAQEYGMQFVTGDIFISTDAD
                     TKLDPNFVKYVEECFADETVTAVAGYVKSIRYNWLTACRALDYVIAQNLDKMAQYALG
                     FMFVIPGAAGAFRTDTFKRLIGFDHDTLTEDLDFTYKLNKYNLKIVYDRRAVSYTQDP
                     SGLGSYINQMRRWYAGGWQNLLKHFGLPEKPGMGLTLSLSYSEGLIFSSLQLILPFIN
                     IVYAMTFLVSYAIFGLILATYASLKEKRLDFFGALPGYIVLRYVNAWLFIEQFIMEVI
                     FKRKNLNWYKPDRVTM"
     gene            2535..2729
                     /locus_tag="VI981_01790"
     CDS             2535..2729
                     /locus_tag="VI981_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479068.1"
                     /translation="MKIILSLLVSVIATSFVLATATVILQASIRSDVSNAKLMTKSGW
                     SPGLERARAAEPLERALESK"
     gene            2768..5245
                     /locus_tag="VI981_01795"
     CDS             2768..5245
                     /locus_tag="VI981_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY4479069.1"
                     /translation="MKSKYKIAVVVGIFLSILIGAQVSYAAEGPNLISNPSVEAGTVS
                     YPTDWLKKDLSGVRGIFLYPVPGYDDARALKLNLYQDTSDGEGWYFKDVSVTPGETYI
                     YRDVYVSNVPTWVRVTFTYKNGSRRSQPSVKFPRRYAWTPMELEIQVPADAIKVTVLH
                     LLKNRGTLTTDMFSLRQVTDIETPVTAPIISSFSATPSSIAIGNSSTLSWQVTNASSI
                     SIDQSIGPVSALGSRLVTPSANTSYTLTASNSGGSVSQTVTISVTNEPPPPPPPTPVP
                     PTLNSFSASPSSITVGGNSTLNWDISNATSILIDQGIGAVAAQGSTVVSPTQTKNYTI
                     TASNDAGTVTRSATITVTPAPVLPVINSFGALPTVITAGESSTLSWSVSNADTIAINN
                     GVGPVVPSGTANVSPSSSVTYTLTATNGVGSVTASASVTVNPVVPPPPPPPPSGSNLI
                     PNPSFEDAAGVDPIYWHRGNWGTNNVTFTYPVTGQSGSKAAEITISSISSGDAKWYHD
                     NVNIEPNTSYIFKDFYKGTTDSNVTIRYTHTNGSESYVWLGTVSPSSLWQEFMRTFTT
                     PADVASLTFFHVISSVGTLTVDNYSLVKGNAPTGTNLFDRGYVTLAFDDGWTSQYDNV
                     LPMLAAAGLKASFFIITNEIAEADQTPGYIYMNSTQLTTVQSQGHEVSAHTKTHTSLT
                     SLSSTAARTEIEGSRSALLSIGMTPVETFVYPYGDYSSAVIQLVQNAGFIGARSVETG
                     YNTKTTNKYALQSQNLVISTTLNEVKTWIDEANRTKTWVILTIHEVNNSNNEYNTPPS
                     VMQGIVNYLGSTNTSVITMREGLLLMQ"
     gene            complement(5421..6260)
                     /locus_tag="VI981_01800"
     CDS             complement(5421..6260)
                     /locus_tag="VI981_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479070.1"
                     /translation="MNESTNELDFTSLTMIEDVKREAVRLAFHEYRAWTISILIVGLF
                     FIYLFPPVVVVIFVWAGYIYSKAHKSFMQQFARCANMTYTDSIEMHEVKGRLFDLGHS
                     NRISNVISGMYEGHAVRLFNYTYTIGSGKHKTTYHFTVHEVSFDKTQFPYILLQSRTM
                     SKYAWIDMTGNKDIEIKLEEEFKDAFRLFAKENYEIEILEIFTPEVLHFLKDDAPQYS
                     IEFAENRLYVYDNTFVSTKSALVRMHDVVKRIVSSLGPRLERMHDDFSALHEYYGDDR
                     KMS"
     gene            complement(6315..6626)
                     /locus_tag="VI981_01805"
     CDS             complement(6315..6626)
                     /locus_tag="VI981_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479071.1"
                     /translation="MKTQSKEGTPTGIRLARIKSDMTVDFGEGAISVNHRTVEKYQDC
                     EVGTLLYRELRQVEGCERMLPEIRPVPASVLPRVLREFSAKGGNGWYSPDLESYEFVD
                     R"
     gene            complement(6892..7701)
                     /locus_tag="VI981_01810"
     CDS             complement(6892..7701)
                     /locus_tag="VI981_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY4479072.1"
                     /translation="MQKIFGAVVVAAGHGTFNGRAKILEEVDGIPMISRIVRAVRAVS
                     TGTCAVVVNPRFGREVTSTLAGTNDNHCAMVVQPKRFGTAEAVERALPRLKKEGVTDF
                     LALYADMPLWSPDTFSSLMQLHCAHDADLSMVTIALTNAHPAVVQRYGRIMRDPSGGI
                     DRIFEVGQLQESSPFTGSVSPSLFVIKLSWFEAHSAKIPVTVHKRDGYPEERWLPSFV
                     GITHDVGGKIADLELADANEALGVNTPHELAFVREIARQRQKNSEIQGASI"
BASE COUNT         2092 a         1887 c         1759 g         2017 t
ORIGIN      
        1 tgtcgaggga aggagggagc gcgcctttcg cccttgctct gcggtatgct tcggctccaa
       61 aaaaacgaca ggagtccggg gtgaaaatct catcaccgag aacatcaccg aagatttcga
      121 gttttccgtc ggcgatcaga atgtccttgg tcattgcatg gtggcgggcc acctgtgcga
      181 tctcgagaac taatttactg cgcccgggaa accgcttctc gacaagagcg tgatggatgt
      241 ttttgtcgtg cccctccttc tcctttgttg agggagcata gagtggaatt ggcaactcgt
      301 cgccttctcg caggccatcc ggcagtttgt ggccacagac aaccccagaa tctctctggt
      361 agtcgctcca gccggaaccg agaagaagaa accgaaagat ctcttcggta tccgtgccgc
      421 ttaggtcctt tgccaccttc gccactgctt ggatatcggg gcggccacgt atctcggccg
      481 gcaaataccg atctacgtcc gccccgagtg ccacattatc cgtcttgaaa tagggcgaga
      541 ggaatccgtt ccaatagtga tgaattgccg agagtgaagg tcctttgtat tctattttgt
      601 caggaaggac gaagtcgaat gcgctgacgc gatttgttgt cagtgaaaga aacttgtcct
      661 cgtgtccgtc cggcagagcg tactgatttc gaaccttacc gcgtcccagt agtcgcagac
      721 cgatttctac ctctccggcg agtgctgatt ttggaatatg aggcatgttt ttgctttctc
      781 tttcgaatgt ttgtgttcat ggagcttccg ttgcggttgc cccgtctgca gatcattcta
      841 tagacgagag taggaaagtc aacgatagca acaataccct aaccctgtga aatgccattt
      901 cgtgtagtat ttactttacc ccgttagaag ccgcgatcgc ggatcgcgat ctgcttctaa
      961 cggggtttac tttttcgtat atttaggtaa agtatgttta acaggttgtg cagggaaacc
     1021 acggctctgt tgacacaatt tagggctatg cacctcgttt atatggtgta tgccaagaga
     1081 agaagcgtat ctcgggtcaa cgaacgcgat gaaaattagc ctattgattc cctgctataa
     1141 cgaagaaaaa acgctccacg cgtctatcca atcatggctc ctacaaacga ggagaccgga
     1201 ccagattctt gtcattgacg actcaagtac ggacaatagc tcgcgtatac tggcatcata
     1261 tgctccttac attgaaacaa gacgaacacc gcaaaatctc ggcaacaaaa gttacgcgca
     1321 agaatacgga atgcagtttg tcacaggaga catttttatc tcaaccgacg cagacactaa
     1381 actggacccg aattttgtaa agtatgtcga agaatgtttc gctgacgaga cagtaacggc
     1441 tgtggcagga tatgttaaaa gtattcggta caactggctg actgcatgcc gagcacttga
     1501 ttatgttatt gctcaaaatc tcgacaagat ggcccaatat gcgcttggat tcatgttcgt
     1561 aatcccaggc gcagccggcg cgtttcgaac agatacgttc aaacgcctga tcggatttga
     1621 ccacgataca cttaccgaag atctcgattt cacatacaag ctgaacaagt ataacttgaa
     1681 gattgtttat gatcgaagag cggtatccta cacgcaggat ccatcggggc tcgggtcgta
     1741 tataaatcaa atgcggcgct ggtatgcggg aggatggcaa aatctgctga aacattttgg
     1801 tcttccggaa aagcccggca tggggctgac actttcactt agctattctg aaggtctcat
     1861 cttttcgtcg cttcaactta tcttgccctt catcaacatt gtttatgcaa tgacgttttt
     1921 ggtgtcttac gctatctttg gattgatact ggccacgtat gcatcactaa aagagaagcg
     1981 ccttgatttc ttcggggcac tgccaggata catagtgttg cggtatgtca atgcgtggct
     2041 atttattgag caatttatta tggaagttat tttcaaaaga aaaaacctga attggtacaa
     2101 gccagatcgg gttactatgt aattatatgt ttataccttt ccccggaatt tcaatattca
     2161 tttaccttgc catcgtgggt tttaagatga tgacaaaatt agcaagacgc gtgatacatt
     2221 tcattgtatg aatcccgtta gaggtcacat cgctatgttt tttgatcaaa tgtacaaaaa
     2281 aatatcaagc ttattaagcc taaaacaaca gaaagtacga cggaaatggt taaacatgtt
     2341 tcgatatttg acttcggatt tattagcaac ggtaattggg aagtgctttg caagtagcac
     2401 tagcatttcc tacctgcccg ccacagccag agcgaaatac gcagtaattt taataaaatg
     2461 aagatgacat taccaattaa ttactctacc tcccattgtg tgtgtcaggc gatggcaggc
     2521 gggcctctaa cgggatgaaa atcattttat cattactcgt ttcagtgatc gccacttcat
     2581 tcgtacttgc gaccgctact gttatattac aggcaagtat ccgaagcgat gtgtcaaacg
     2641 caaaattaat gactaagtcg ggctggagcc caggactaga acgagctcgc gcggccgagc
     2701 ctctagagcg tgcacttgag tcaaagtaac gaacggtaaa ctatcaattt taaaaatact
     2761 cataataatg aaatcaaaat ataaaatagc ggttgtagta ggaatctttc tttcaatatt
     2821 gatcggagcc caagtttcct acgcggcgga agggccgaac ctaatttcaa atccgagcgt
     2881 tgaggcagga accgtgtcct atccgactga ttggcttaaa aaagatctct cgggtgtacg
     2941 aggcatcttt ttataccctg taccgggcta cgacgacgcg cgagcactga agctcaatct
     3001 ttaccaagat acaagtgacg gcgagggctg gtatttcaag gatgtctctg tgacaccggg
     3061 cgagacatat atctacaggg atgtatacgt gtccaatgtg ccaacctggg ttcgagtgac
     3121 tttcacgtat aaaaacggaa gtcgacgttc ccagccttca gtgaagttcc cgaggcgata
     3181 tgcgtggaca ccgatggaac ttgagataca agtacccgct gacgcaataa aagtcaccgt
     3241 tctccatctt cttaaaaaca gagggacgct gaccacagac atgttctctc tgcgacaagt
     3301 aaccgacatc gagacaccgg ttactgcgcc catcatttca tcgtttagcg caactccgag
     3361 ttccatcgcc attggaaata gttcaacact gtcatggcaa gttactaatg catcctcgat
     3421 atcaatagat caaagtatcg gccccgtatc tgctttagga tcacgacttg ttactccgag
     3481 cgcgaacacg tcatacacac tgactgcatc aaattctggc ggcagtgtca gtcaaacggt
     3541 aactatttca gtaaccaacg agcctcctcc gccaccaccg cctactccag taccgccgac
     3601 ccttaactca ttcagtgcat cgccgtcgtc tatcacggtg ggtggaaatt caacccttaa
     3661 ttgggatata tccaatgcaa caagcatctt aatagatcag ggtatcggtg ctgtcgcggc
     3721 acagggtagt acagtggttt ctccgacaca gacaaaaaac tatactatca cggcctctaa
     3781 cgatgcaggt acagtcacgc gaagtgcaac cattactgta acccctgctc ccgttcttcc
     3841 agtcataaat tcgttcggag cgctcccgac cgtcattact gccggtgagt cctcaactct
     3901 ctcttggagc gtctcgaatg cagacaccat tgccattaac aatggagtcg ggcccgtcgt
     3961 accatccggt acagcgaatg tcagcccaag ctcgagcgtt acctatacat taactgccac
     4021 aaatggagtt ggatcggtca cagcatctgc gagtgtcaca gtgaatcccg tcgttccgcc
     4081 tccgccgcca cctccgccgt caggatcgaa tctgattccc aacccatcat ttgaggatgc
     4141 tgcgggcgta gatccaatct attggcaccg tggaaattgg ggaactaata acgtcacatt
     4201 cacgtacccc gtaactggac agagtggcag taaagccgcg gaaattacta tctcgagcat
     4261 ctcgagcggg gacgctaagt ggtaccacga taatgtgaac atagagccca atacgagcta
     4321 tatattcaag gatttctaca agggtaccac cgatagtaac gttacaattc gctacacaca
     4381 tacgaatggc agcgaaagtt atgtttggct tggaaccgtt tcgccgtctt cattatggca
     4441 ggagtttatg cggacattca cgacgccagc tgatgtcgcg agcctcacat tctttcatgt
     4501 gatttcaagt gtaggaactc tcactgtcga taattattcg ttagttaagg gaaacgcgcc
     4561 gactggcacg aatctgtttg atcgtggcta tgtcacgctc gcgttcgacg acggctggac
     4621 atctcaatac gataatgtac tgccaatgct cgcggcagca gggttgaaag ccagcttctt
     4681 tattatcacc aatgaaatcg ccgaggcaga ccagaccccc ggatacatat acatgaattc
     4741 aacccaactc accactgtgc aatctcaagg ccatgaggta agtgcgcaca ccaagacgca
     4801 cacatcgctg acgagtcttt cgtcaacggc ggcgcgaact gaaatcgaag gttctcgaag
     4861 cgctctctta tcgattggca tgacgccggt tgagacattt gtctatccat acggcgacta
     4921 tagcagcgca gttattcaac tggtacaaaa tgccggcttc atcggagctc gcagtgttga
     4981 gacaggctac aacactaaga caacaaataa atacgccctc cagtctcaaa atctcgtcat
     5041 aagtacgaca ctcaatgagg ttaagacatg gatcgacgaa gcaaacagga ctaagacatg
     5101 ggtcattctg actattcacg aagtaaataa ttcgaacaat gaatacaata cgccgccgtc
     5161 agtgatgcag gggattgtca attatcttgg atcaacaaac acgtcagtca ttacgatgcg
     5221 tgaagggttg ctgttgatgc agtagagggc gctcggatta atcaatttta aaacacgtat
     5281 aattttctgc taaaaattta ctatgctctg gccgacgccc tccgcaatgt ttaaaaattg
     5341 ataatcctcg ctcagtcgta gaagaagtga atcggaccgt cgacaacaaa aaaacccttt
     5401 caaagagggt ttttttgttg ttacgacatt tttctatcat cgccataata ttcgtgcaat
     5461 gcggagaaat catcgtgcat gcgctcgagt cttgggccga gcgaggacac aatacgttta
     5521 accacgtcat gcatacgaac taaagctgat ttcgtagata caaatgtatt atcgtagaca
     5581 tagagtcggt tttctgcaaa ttcaatgctg tattgcggcg catcatcttt aagaaagtgt
     5641 agcacctccg gtgtaaatat ctccaaaatt tctatttcgt aattttcctt tgcaaaaagt
     5701 ctaaatgcgt ccttaaattc ttcctcgagt ttaatttcga tatctttatt gcccgtcata
     5761 tctatccaag cgtactttga catcgtgcgc gactgaagca atatgtacgg aaactgcgtt
     5821 ttatcgaaag aaacctcatg aacggtaaag tgataggtag ttttgtgttt gccacttcca
     5881 atcgtatacg tgtaattaaa aaggcgtact gcatgaccct catacatacc ggaaataaca
     5941 ttggaaatcc tattggagtg cccgagatca aagagtctcc ctttcacttc atgcatttct
     6001 atcgaatcgg tgtaagtcat attcgcacac cgagcaaatt gctgcataaa actcttatgg
     6061 gctttggaat agatatatcc tgcccacacg aaaataacta caaccaccgg cggaaacaaa
     6121 taaataaaaa atagtccgac aatcaaaatt gatatcgtcc atgctcgata ttcgtgaaag
     6181 gcaagacgaa ccgcttctcg ttttacatcc tctatcattg taaggcttgt aaaatctaat
     6241 tcatttgttg actcattcat gttacggaga gaattttaac acgcataaac aaaaaagact
     6301 ccggcgtttg gagtctatct atcgacaaat tcgtaactct ccaaatctgg agagtaccag
     6361 ccgttcccgc cttttgcgct gaattccctt aggacacgcg gcaggacgga agcggggacg
     6421 ggacggatct cagggagcat tctctcgcac ccttctactt ggcggagctc acggtacaag
     6481 agtgtaccaa cctcacagtc ttggtacttt tccacagtcc gatggttaac tgaaatggct
     6541 ccttcgccga aatcaaccgt catatcactt ttgatccggg caagcctaat ccctgtaggg
     6601 gtgccttctt tgctttgagt tttcatagtt tggtgttctt gagccaaatt tacgctacca
     6661 tataatttaa ttaatgggaa gtacgggaga accccacact aattttgcac gatacttcac
     6721 acagactcat gcttcgccag taagggaggg cctattcggc cgcgcattcg ttgttttcaa
     6781 tattaggtct gtatctattc gagctttttt atggaaaata aaggcaaaat tagtacgggg
     6841 tttgtatttc gcaaccacca catgcgtgga cgaaatacaa aaaaggcgcg gtcaaatcga
     6901 cgcgccttgt atttccgagt tcttttgacg ctgccgtgcg atctcacgca cgaaggccag
     6961 ttcatgaggt gtgttcacac cgagggcctc atttgcatca gcaagttcga gatcagcaat
     7021 cttgccgcca acgtcgtgag taatgccgac aaaactcggc aaccagcgct cttcagggta
     7081 tccgtcacgc ttatgtacgg taaccggaat cttcgcagaa tgcgcctcga accacgagag
     7141 cttaatcaca aacagcgaag gactgaccga cccggtaaac ggactgcttt cctggagctg
     7201 tccgacttca aagattcgat cgatgccgcc acttggatct ctcatgatgc gcccgtacct
     7261 ttggacaaca gcgggatgag cgttcgtgag cgcaatcgtt accatggaga ggtcggcgtc
     7321 atgcgcgcaa tgcaactgca taagcgagct aaacgtgtcc ggactccaca gtggcatgtc
     7381 agcgtacaga gccaaaaagt ccgtcacgcc ctcctttttc agccgaggaa gagcacgttc
     7441 cacggcttcg gccgtgccga aacgtttggg ttgaacgacc attgcgcaat ggttatcgtt
     7501 tgtacccgca agtgtgctcg taacctctcg gccaaaacgg ggattcacaa ctacggcgca
     7561 ggttccagtg gaaactgcgc gtactgcacg cacgattctc gaaatcatgg gtataccgtc
     7621 aacttcttcg agaattttcg ctctgccgtt gaatgtgccg tgtcctgccg ccaccacaac
     7681 tgcgccgaat atcttctgca tatgatgtcc tttccttgtt cagattttgg tttaaaccga
     7741 atgccatcag gccca
//
LOCUS       DATDSU010000055         5918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1395213, whole genome shotgun sequence.
ACCESSION   DATDSU010000055 DATDSU010000000
VERSION     DATDSU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395213"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VI981_01815"
     CDS             <1..449
                     /locus_tag="VI981_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479073.1"
                     /translation="TYLAYTSDRWYMLGRACPRDRTRPILLVAGKWYHFKMINSIRRI
                     ALTLAIIGGLTLGAGVFMTSSSPCGSAYCTALALVSNTRFDQTAVQSTPRTDSVKSPN
                     YRVIAEAMQVTSLYIVFPAILLLLGLEVYKPYFLRHHMRHRHAHIK"
     gene            complement(442..1206)
                     /locus_tag="VI981_01820"
     CDS             complement(442..1206)
                     /locus_tag="VI981_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY4479074.1"
                     /translation="MNISPIILAAGHGKRMQSALPKPLVTYRGRPIIEYAIESVEKAH
                     FEHATTIVIAPDEQGKQIKDATKSLGTLEYVIQKERLGTGHAVLACREALEKTALNVL
                     VFYGDHPLVSAKTWKDLATIHLEKKADVTLVTVKLKDYDEWRKPLYDFGRIIRDADGR
                     IDRIVEKVDASAKQLEIKEVNPGFYCFRADWLWKHLPMLKNENSKGEYYLTDIIGLAV
                     LEHAVITDIQINPKEALGVNTKEQLALIETIDHAAT"
     gene            complement(1464..1910)
                     /locus_tag="VI981_01825"
     CDS             complement(1464..1910)
                     /locus_tag="VI981_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479075.1"
                     /translation="MANVIQMQRRAIESLRPKAQQGLVAIKDFFVMPKPLDFRLKPHF
                     PLFLYGISVGIGTCSDIHGMNVGVFGLRGPGIQVTSDLRTELTRCEFFVKLRPLTVAE
                     TQERELAQGLRHTHELRLLSCAEVELNGSVTCLFDDTGVSSFYVTI"
     gene            complement(2261..2332)
                     /locus_tag="VI981_01830"
     tRNA            complement(2261..2332)
                     /locus_tag="VI981_01830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2298..2300),aa:Val,seq:gac)
     gene            complement(2390..3001)
                     /locus_tag="VI981_01835"
     CDS             complement(2390..3001)
                     /locus_tag="VI981_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479076.1"
                     /translation="MAKYKNKRVAPLTIITLACLVLVLVLSAIGFIYTKNDQTAIIKS
                     ADQFKWQEFSDPSSGLSFKYPKIWGDAKVDSWPKGTVISLKDDSFAIELLSSSLTIDP
                     KTKRVLSPTEIIDENYNAHLKETDVSDLEKDTITLDKRSAVRITYLSTAYPVKSRVVG
                     VSLRKSYRDEFFSLYYVYPVAQENDESVILSQILASLKFNPSE"
     gene            3185..4441
                     /gene="creD"
                     /locus_tag="VI981_01840"
     CDS             3185..4441
                     /gene="creD"
                     /locus_tag="VI981_01840"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEY4479077.1"
                     /translation="MKYDTISVKILVIALIGGICFVASLLVMGLTGERQNRFSEAKQE
                     IVDKWSKQQVIVGPMIIKKTGTEGVHTYVLPETLSYEVVLEPETRTRGIFKSVVYTSK
                     IKVRGEFVGDAIGRDAGTVMFSLPITDTRGIENQFDLTWNGVKYPLNPGPGVAFKERS
                     GLNAAVPINSKVSKIPFSFELQLKGSESMSIAPLGKETVIAMSSPWQSPKFVGAFLPS
                     EREVTESGFKSEWRISSFGRSFPQTWQTGDVNLQQLVDSGAGVELHEQVDGYDMTFRS
                     VKYAILFIVITFAAFFLFDVRARVRIHPVQYLLIGSALSLFYLLLLSLSEQIGFLSAY
                     LIATAMIALLVTMYSAFVLKSKQRAFPIFAILVVLYGYLYFVLRLEDYALLFGSLLLF
                     ALLAVIMFMTRHIDWFALGKEKEGRD"
     gene            complement(4780..4851)
                     /locus_tag="VI981_01845"
     tRNA            complement(4780..4851)
                     /locus_tag="VI981_01845"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4816..4818),aa:Glu,seq:ttc)
     gene            complement(4830..5087)
                     /locus_tag="VI981_01850"
     CDS             complement(4830..5087)
                     /locus_tag="VI981_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479078.1"
                     /translation="MYFTYILRSIRGKYYIGSTRDLYKRIVLHNTRRVKSTKYGSPWK
                     LLYFEKFNNLSDARNREIQIKHWKSRVAVEQLINTAQSSNG"
     gene            5319..5405
                     /locus_tag="VI981_01855"
     tRNA            5319..5405
                     /locus_tag="VI981_01855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5353..5355,aa:Ser,seq:cga)
     gene            5424..>5918
                     /locus_tag="VI981_01860"
     CDS             5424..>5918
                     /locus_tag="VI981_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479079.1"
                     /translation="MYKYKHPKPIVIKLTDELGFRLRQKAAEYIATNQNRTGAERGSS
                     EEQGFGALAEMVIRNKLGMPEINPEDHPLGHDLLLPSGIKVDVKCRGGALPFKEEYES
                     SDGIAREAKHNFFARQMHDERLDADIYVMTHLETPSKRELPGTTRQRKWILYICGWVS
                     KERVA"
BASE COUNT         1589 a         1290 c         1293 g         1746 t
ORIGIN      
        1 aaacctacct agcatatacc agcgatcgct ggtatatgct aggtagagct tgccctcgag
       61 ataggacgag gcctatttta ttagtcgctg gtaagtggta tcattttaaa atgattaatt
      121 cgattcggag aatcgcgctc acgcttgcta taataggcgg cctcactctt ggagcaggag
      181 tgttcatgac gagttcgtca ccctgtggaa gtgcatattg cacggcttta gcgctcgtat
      241 ctaatacgag gttcgaccaa actgccgtac aaagtactcc gcgaacagac tctgtaaaat
      301 cacctaatta tcgggtgatt gcggaggcaa tgcaggtcac aagcctttac attgtatttc
      361 cagctattct acttttgctt ggactcgaag tgtataaacc ttattttcta cgacaccaca
      421 tgagacatcg gcacgcacat atcaagtagc cgcatgatca attgtttcaa tgagcgctaa
      481 ttgctcttta gtgttgacac caagtgcttc tttcggattg atttgaatat cggtgattac
      541 ggcatgttct agaaccgcaa ggccgattat atcagttaga taatattccc ctttagaatt
      601 ttcattcttt aacatcggta aatgcttcca tagccagtct gcgcggaaac aataaaatcc
      661 cggattaact tcttttattt caagctgttt cgcactggcg tctacttttt caacgatcct
      721 atcaattcgt ccatcggcat cgcgtataat gcggccgaaa tcataaagtg gttttcgcca
      781 ctcatcgtaa tcttttaatt tcacagtcac gagcgtaacg tcggcttttt tttcaagatg
      841 aattgtagca aggtctttcc atgttttcgc ggatacgaga gggtgatctc cgtaaaatac
      901 gagcacgttt aaggcggttt tttcgagcgc ttccctgcac gcaagcacag cgtggcccgt
      961 acccaaacgc tctttttgta tgacatattc gagtgttccg agtgactttg ttgcgtcttt
     1021 aatctgtttg ccttgttcgt caggggcaat gacaatagtg gtggcatgct cgaaatgtgc
     1081 tttttcaaca gactctatag cgtattcaat aataggcctt ccgcgataag taactagcgg
     1141 tttaggcagg gctgattgca tccgctttcc atggccggca gcaagaataa tgggggagat
     1201 attcatgggg tagtgaaaaa tggcatcgct ttggaaaagc acatgccgct gttattactg
     1261 agaattgacg tatttaagtt agtcatttca tataaagttc tattgaactc caatgccatt
     1321 ttctactacc ccattcatat ttttgtcaca agggttacat tctatcagaa gatacttttt
     1381 tgttaagcaa acatcgaatc ccgtcttttg aacgtatatg tcaggatttt tctcaacaaa
     1441 aaaaatcgcg tttgaaacgc gatttagata gtgacgtaaa atgaacttac accagtatcg
     1501 tcaaagaggc aggtaacgga gccgttgagc tcaacttcgg cgcacgagag cagcctaagt
     1561 tcatgcgtgt ggcgtaagcc ttgtgcgagc tcccgttctt gtgtctccgc tacggtcaga
     1621 ggtcgcagtt tcacgaagaa ttcgcaccga gtgagttccg ttcgtaagtc gcttgtgact
     1681 tgaattcccg gacctcttag tccgaacacg ccgacgttca ttccgtgtat gtcagaacat
     1741 gtaccgattc cgacactgat accatacaag aaaagtggaa agtgaggctt cagcctgaaa
     1801 tcaagcggtt ttggcataac gaaaaaatct ttaattgcta caagcccctg ttgtgctttg
     1861 ggccgcaatg attcgatagc tcgtctctgc atttgaatga cattagccat agttccagtt
     1921 cctccttgtt tgttgttgaa agagcaaaaa caaaaatcag cttcccaaga aagctgattt
     1981 ttgttttaaa agtacgaaat ttttaactca aagaccagct ttcttggcga gtctccatca
     2041 tcatgatctt tgaattttct actgtctcgg tcacgttctt gattatatac acgagcgttc
     2101 tattgtcaat cgtcaggtct gtgctcgctt cgctgtgcgc gctctgggga ttggctatta
     2161 cttagccatg tgcttttcta tctcgcagac tcgataaaga aaagcctttc aaccccctca
     2221 cgcagcaaag aagtttgctg ctatcgtgct cgcttcgctg tgcgcgctga ggggattgaa
     2281 cccacggccc tcgtcacgtc aagacgatgc tctaccactg agctaagcgc gcttttggta
     2341 aagcctatct tatcaattat tgttttgaag gcaaacagcc aaagaagcct tactcactag
     2401 gattaaattt caaactcgca aggatttgac ttagtatcac ggattcatca ttttcttgtg
     2461 cgaccggata gacataatag agactaaaga attcgtcacg atacgatttt ctcaaagaga
     2521 caccgacaac tcggctcttg actggatatg cggtagataa gtaagtgatt cggacagcac
     2581 ttcttttgtc gagcgtaatt gtatcttttt ccaaatcgct cacatctgtt tccttgagat
     2641 gagcgttata attttcgtcg attatttcag ttggtgacaa tactcttttt gtctttggat
     2701 ctatcgttag gctcgaactt aaaagttcaa ttgcaaaact atcgtctttc aaggatatta
     2761 ccgttccctt gggccacgaa tcaactttcg cgtcgcccca tattttggga tatttgaatg
     2821 aaagacctga cgacggatct gaaaactcct gccacttgaa ttgatccgca gattttatta
     2881 tggctgtctg gtcatttttt gtataaatga accctatggc gctcaaaacc aaaaccaaaa
     2941 ccagacaggc aagggtaata atggttagcg gtgcaaccct tttattcttg tactttgcca
     3001 caaaaataga ataccacaat cccacagtaa agaaatgtgg aaactaatta tgaaggcttt
     3061 ccttcggtcg gaaacttaga tgcgtttaag tacactgtcg aatcggctat tgtttccgag
     3121 taaagggtaa aatgaaactc tttcctccat ttggagttat atagatatag ataatcttat
     3181 cgatatgaaa tacgacacta tctcagtaaa aatcttagtc attgctctca ttgggggcat
     3241 ttgttttgtt gcgagcttac ttgtcatggg acttactggt gagcggcaaa atcgtttttc
     3301 tgaagcaaaa caagaaatag tcgacaagtg gagcaagcaa caagttatcg tggggcctat
     3361 gattattaag aaaactggca cagaaggcgt ccacacatat gttctgcccg aaacgcttag
     3421 ctatgaggta gtgctcgagc ccgagaccag aacgcgcgga atctttaaat ctgttgtata
     3481 tacgagcaag attaaggtga gaggagaatt cgtaggtgat gcgattggcc gtgacgcagg
     3541 cactgtcatg ttctccttac cgattacgga caccagagga attgaaaatc aatttgacct
     3601 tacgtggaat ggtgtgaagt atcctttaaa tccaggtccg ggagttgcgt ttaaagagag
     3661 atccggtttg aatgcggcag tgccgattaa ttctaaagtt tcgaaaatcc cattctcatt
     3721 tgagctccaa ttaaagggta gcgagagtat gtctatcgca ccgcttggca aagagacagt
     3781 gattgcgatg tcctcgccgt ggcagtcgcc aaagtttgtg ggagcctttc ttccttcgga
     3841 gagggaagtg accgagtcag gtttcaagtc agagtggagg atttcatcgt tcggaaggtc
     3901 cttccctcaa acatggcaga ccggagatgt gaatctgcaa cagctcgttg actcgggagc
     3961 aggcgtggaa ctacacgagc aagttgacgg atatgatatg acttttagaa gtgtaaaata
     4021 cgccattctc tttatcgtaa tcacttttgc cgccttcttc ctctttgacg tgcgggcgcg
     4081 ggtgcgtatc catcctgtgc aatatctgct tatcggttct gcgctctcgt tgttctattt
     4141 actcttacta tcactatctg aacaaatcgg gttcctctct gcctatttga ttgcaactgc
     4201 catgattgca ctcctcgtga ccatgtatag cgctttcgtg ttaaagagta aacaaagagc
     4261 gtttcctatt tttgctatcc tcgttgtttt atacgggtat ctctattttg tcctaaggct
     4321 tgaagattat gcactgctct tcggctcgtt gcttctcttt gcgcttcttg ccgtcattat
     4381 gttcatgact cgtcacatag attggtttgc actcggaaaa gagaaggaag ggcgagacta
     4441 attttttgcg atccatggga agtcggctat ctgtactttt ctgctgaaaa gtcagacagc
     4501 ccatccttac aggacagggt atttgatact ttatggcgcg cttcgcgctc aagattatct
     4561 gatcacaaat atttttactg acgggaaaat ttcgcgatcc ttacaggatc agtacacctt
     4621 ttggctctcg acgactcgag ctcaaaaggt cttgcgaagc tccacgtatt cgcttctcac
     4681 ccagctcggt atttttgcct ttgcttggca aaacaaaaat atacctgcgc cttcacaaaa
     4741 tcacaaagtc tgccgtgtgg aagactttgt gtatgtttgt gaccccacgg ggagtcgaac
     4801 cccggttaac ggattgaaaa cccgttgtcc taaccattag acgattgggc cgtatttatt
     4861 aactgttcaa ccgcaaccct acttttccaa tgttttatct gtatttctcg gttcctcgca
     4921 tcgcttagat tattaaattt ttcaaaatat agcaacttcc acggcgatcc atactttgta
     4981 cttttcactc tcctcgtatt atgcaagaca attcgtttat atagatcgcg tgttgaacca
     5041 atataatatt tccccctgat cgatctcaag atatatgtaa aatacatttt agtcgattcg
     5101 aaccgatagt cccgattttg atttatcaaa atcgaggatc tcccgacgca tcgcggatcc
     5161 tcgacattgt aaacaatgtc gggactcgat ttcctaaagg aaatcgggaa cgatggggcc
     5221 gcataacagc ctatagctta cggttatagt tgttagggca aaaaaactat agcatttttt
     5281 gaataaaccg caaaaaattg ctattatctc gacaccttgg aagggtgttc cgaattggta
     5341 aggagccgct ctcgaaaagc ggtgcccgca agggcgtgga ggttcaagtc ctctcccttc
     5401 cgccagattg gcaactttga accgtgtata aatacaagca tccaaaaccg attgtaataa
     5461 agctcaccga tgagctaggt tttcgattga ggcaaaaagc ggctgaatat attgctacaa
     5521 atcaaaatcg aaccggcgca gaacgaggaa gttcagagga gcaaggattc ggtgcacttg
     5581 cagaaatggt tatacgaaac aaactaggta tgccggaaat taaccccgaa gatcatccgc
     5641 ttggacatga cctcttgctt ccctctggaa taaaagttga tgttaaatgt cgcggtggcg
     5701 cactgccatt taaagaggaa tatgaaagca gtgatggtat tgctagagaa gcaaaacata
     5761 atttctttgc gcgacaaatg catgacgaaa gactagatgc tgatatttat gttatgactc
     5821 acctcgaaac tcccagcaaa agagagcttc cgggcacaac tagacaaaga aaatggatac
     5881 tttacatttg tggctgggta tcaaaagagc gagtagcg
//
LOCUS       DATDSU010000056        11295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_717236, whole genome shotgun sequence.
ACCESSION   DATDSU010000056 DATDSU010000000
VERSION     DATDSU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717236"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VI981_01865"
     CDS             complement(<1..435)
                     /locus_tag="VI981_01865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY4479080.1"
                     /translation="MDTKKIYDELKHEIEGLRSGPEVVHVGEVVRVSDAVALVRGLSS
                     VKYSEIVEFVVPGAKKPIYGLAQNLEEDQVGVIILGDASLIAEGHTVKSTGEILSIPV
                     GPELIGRVVNPLGEPIDGGPSLGLGMKRYPLEKIAPGVIARES"
     gene            complement(902..1276)
                     /locus_tag="VI981_01870"
     CDS             complement(902..1276)
                     /locus_tag="VI981_01870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY4479081.1"
                     /translation="MKLNSKQYGLALYKLTHRKSEHEMKSILHAFARFLFSNGDTSKV
                     KDVVRFYDETKLKAEGRIRSRVRSAYGDENVLPRHMHGTELYVEQEKDPNLVAGITVD
                     VGDLRIENSIDKRLRDLKTALS"
     gene            complement(1286..1795)
                     /gene="atpF"
                     /locus_tag="VI981_01875"
     CDS             complement(1286..1795)
                     /gene="atpF"
                     /locus_tag="VI981_01875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY4479082.1"
                     /translation="MGELVHSLGIEWKVLIAQIINFAILFYVLKRFAVGPIMKILDKR
                     FLRMESDRAEAEKIESKVADMEKEKEAILIVAREESQKIIKNAEAVAAKLRTERVAAA
                     EKEVAAIVAAGKKQLADETKNAETVLRREMGSLIAIAVEKTVAKAMDKKAHEYLVEEA
                     RQVLMGLEK"
     gene            complement(1803..2015)
                     /gene="atpE"
                     /locus_tag="VI981_01880"
     CDS             complement(1803..2015)
                     /gene="atpE"
                     /locus_tag="VI981_01880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY4479083.1"
                     /translation="MESIAAALAIGLGAIGSGIGIGLLAARAMEAIGRNPEASGKIQG
                     AMILAIAFTEAIAIYALVVALIIKFV"
     gene            complement(2115..2864)
                     /gene="atpB"
                     /locus_tag="VI981_01885"
     CDS             complement(2115..2864)
                     /gene="atpB"
                     /locus_tag="VI981_01885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY4479084.1"
                     /translation="MVHVSIQSEHLFEIFGFPVTNTLLTGWIVIILLCIFAWVISKRI
                     KEVPDRFQNAVEAFFEGIFNFMAGFAPNRETAKKFFPLALTIFIFVLSANWIGILPGV
                     GSIGFTEIHEGGETFVPLFRSVYSDLNMTLALALIVVTLVHIVGVVTIGLRGHAHKFF
                     NFSSFGNAFAGILELVSEAAKIISFSFRLFGNVFAGEVLLVIIASLVPYLAPVPFLGL
                     ELFVGFIQALIFSVLAMLFLGSATQSHAEAH"
     gene            complement(2958..3200)
                     /locus_tag="VI981_01890"
     CDS             complement(2958..3200)
                     /locus_tag="VI981_01890"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY4479085.1"
                     /translation="MIGTPKTNEQGKDKYKELKLVNFALELGFTIAIPIVLLALLGRL
                     LDRWWGTSPWLLLIGIFVSMIVSIALIYKKTKDVLR"
     gene            complement(3274..3618)
                     /locus_tag="VI981_01895"
     CDS             complement(3274..3618)
                     /locus_tag="VI981_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002175467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479086.1"
                     /translation="MREKKSSKRDQYTIVLEDVRSDFKLFGEELQAVRGSVERLDGKV
                     DQLDGKIDRLDGKVDQLDGKVDRIEGRVVRLEENIEFVKGELTVIRHNQVTRDEFKLL
                     ETRVLRLEKRLK"
     gene            complement(3777..3858)
                     /locus_tag="VI981_01900"
     tRNA            complement(3777..3858)
                     /locus_tag="VI981_01900"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3822..3824),aa:Tyr,seq:gta)
     gene            complement(3821..4462)
                     /locus_tag="VI981_01905"
     CDS             complement(3821..4462)
                     /locus_tag="VI981_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479087.1"
                     /translation="MGVLDQHIERIKKLYYEKGYSMNDVAKHYRVSLDMVVHIMRKYD
                     LKRRSHHEANHQWFSKKIPSYRIRSLQKKYNELKSAGAMLYWAEGYNSPKGKGVDFAN
                     SNPQMIKLFLKFLRTTYELDEKRFRIYLYCYADQDVKGIISFWAETTDIPTSQFLKPY
                     IRQDFRIDGRKMLHGLIHIRYHDKKLLIEIKNLIKYYESKYCVGTQVVNEGAL"
     gene            complement(4579..7272)
                     /locus_tag="VI981_01910"
     CDS             complement(4579..7272)
                     /locus_tag="VI981_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4479088.1"
                     /translation="MTPFNHFTTKAKESIRRAHELAIERGQNHVNPLHLLGALILQEE
                     SMVTSILDKLEVDTILLTDSLLEAIESPESGSVLSPSYQLYLTPELAQAIEGSGKVAA
                     ALKDEFVSTEHLFIALFDVPGNARDILTRFRIEKDGVMRVLEELRNSKVTDVHQPKKY
                     KFLSKYTRSLTKLAGENKLDPVIGRDTEISRIIQILSRRTKNNPILIGEAGVGKTAIA
                     EGLALRMSLGDVPESLKDKELVSLDLGLLIAGTKYRGEFEERLKNIMKEVERSEGKII
                     LFIDEIHTIVGAGAAEGSMDASNMLKPALARGELRAIGATTLKEYQKHIEKDPALTRR
                     FQPVFVSEPSVEDAMAILRGLKEKYELYHGVKITDDSIVAAVNLSSRYITDRFLPDKA
                     VDLIDEAASQLKISLENMPPVLEETRRKVMRLEIEREALKNDPNSKTAKLRVKKIEKE
                     IADLKEKTSELELKWKNEKEIIGGIKSIKKDLEKLRIDAEHAESRADLARAAEIRYAR
                     IPNLEKELESKHKRLKRLQSSRRILKEEITENDIADVVSRWTGIPVNRMLEEEALKLG
                     RMEEFLTKRIVGQNEAVKKIADTVKRSRAGIADPNRPIGSFIFLGPTGVGKTELTKAL
                     AEFMFDDERALIRIDMSEYMEKHSVSKLVGSPPGYVGYDEGGTFTEQLRHRPYSVILF
                     DEIEKAHPEVFNVLLQIFDNGRLTDNKGRVVNFKNSIIILTSNIGAQYIDRMEKMGFA
                     NERNAKSNYEGVKDKVLESLKDYFRPEFLNRLDDIIVFDILSPEAISEIVKIQVNIVK
                     ERLAQKEITLIVGDGALSQLAREGYDPQYGARPLKRIIQNKILNPVASLMISQGIMKG
                     GEITVNVKGSEYVFDVKKGKKGAFFEKEELAEQPEMAEAQA"
     gene            complement(7504..8298)
                     /locus_tag="VI981_01915"
     CDS             complement(7504..8298)
                     /locus_tag="VI981_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479089.1"
                     /translation="MEDLTKQQLILLALLVSFVTSIATGVVTVSLVDQAPAGVSQTIH
                     QIFEKTIERVVPSESIATVTKEIKIPVPPDEALATAVASVSKNFVLIRKEGTDSTASR
                     GLLLDAQGIFVADMGVLKEESGLIAVTKNSNFPLSIVARNDIKQIVYLKADPSLVTRA
                     ELVPVLFKKGEVLLGQNVFILNVEDKLAVFRGIVTALEEKEATPIIHTDISDETSHGL
                     GLFTLSGELVGLGTKKSDILNGQGFISIESIRSDLSNIKEELKSEK"
     gene            complement(8468..10228)
                     /locus_tag="VI981_01920"
     CDS             complement(8468..10228)
                     /locus_tag="VI981_01920"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479090.1"
                     /translation="MKGNKHRLVSVNVKSFYIGVFVAASLFAVTSSVSAGTEHNLSGF
                     GWSGTIGWISFNSIDCDTDGNGIMSSTEASAHAGCPAAGSSVSNYGATLDLSGNISGY
                     IWAETIGWISFDRAETGNPPNSDINSGTGSIAKVNFGSGAVNGWARALSPVSGSNFWD
                     GWIHLSGTNHSSPDATGGGGVTYKASDTTLIGYAWGSDIVGWINFNVTYDVVEYVVTV
                     TKSGSGAISAPGLTCGVFTCTGTYAAGSLVTFTASPDPFSGYTFAGWSGACETAEEEC
                     TISVEAAANLEAHFASASQILSKGPAGSQPVGTTDVDLTVTTDRVSTCKYDTADVSYT
                     SMAYTFGSTLDTTHTENISGLSNGDFRSYFVRCKDATPEAQDSIATLLPYIPDIRNAY
                     ANDPAEQIEFYIDVPSYTLTVVVVGGGTVSGTGGISCEPNCTQVYLENDQVVLTPHPT
                     SGSVFSSWTGTGACSGTGSCTVSMTSDKNVTANFSSASSQSQQKPPNCLAGYRVRASS
                     QAFVYATPESTSLSVTPVPSPNPQSSITLGVVVSMPLNPTFNFDENVWFCYVNFDRGA
                     DGWVKQDLIRIRPTNLYQEG"
     gene            complement(10251..11060)
                     /locus_tag="VI981_01925"
     CDS             complement(10251..11060)
                     /locus_tag="VI981_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015811808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479091.1"
                     /translation="MKTLRQNFFSAVRITVIALVLTLGANLIYSWNAPTTTPPTGNVA
                     MPINVSSAPQGKLGTFGLGLASPDTSNSIKLDVLGKIKIVDGSQGADKVLKSDANGVA
                     TWQALSNISGGGNTCVTIDTANGDSQGRGTINMVINGRNICADNIGCSIHVWAYNLTS
                     NSGADVPGFNYYGSSPQYFKQSEENNRWLYTDNSGGNTNIQGRNGDTGSSADVKILQF
                     DTRESGNRFQLWDDPDSGWSPTVTEAVPASYLTYDDDADDGDNYILNICDY"
BASE COUNT         3042 a         2718 c         2221 g         3314 t
ORIGIN      
        1 ggattctcga gcaataactc cgggagcaat tttctcgagc gggtatcttt tcattcccaa
       61 gccaagcgac ggaccgccgt cgatcggctc tccaagcgga ttgaccacgc ggccgatcaa
      121 ttctggtcca accggaatag agagaatttc tccggtgctt tttaccgtat gaccttcggc
      181 aattaaactc gcatcgccta aaatgataac ccccacctga tcctcttcaa ggttttgagc
      241 aaggccatag attggttttt ttgcaccggg aacgacaaac tcgacaattt cggaatattt
      301 tacactacta agtccccgta caagcgccac agcgtcagaa acacgcacca cttcaccaac
      361 atgaacaact tcagggccgc ttcggagtcc ctctatttca tgtttcaatt catcgtatat
      421 tttctttgta tccatcccgt ttagaaaccc gcctgccatc gcctgacaca ctgatgggag
      481 gacgtgcgat taattggtaa tatgatcatc attttattaa agctaccaca tatttcgctc
      541 tggctgtggc gggcaggtag gaaatgccca agattatctt gaatcggctc ctatgttact
      601 taagctgata atggttcgct cacatgtaat tattgatagc atttccgcga atcggcaaaa
      661 aaaagtttga tacgctgggg tattctgtgt ttttttaaaa tataaattat acataacttt
      721 ttgatttcta acgggatcca tggggtagtg aaaaatggct cgctcttttt ggagcaatta
      781 cgccatcata cttattattg ctgttaattt tttgacttgc atacaatcga attttatttt
      841 tatttctcca atgccatttt ctactactcc atctatgtgg tgaaatttat gctctttgta
      901 attaacttaa tgcagttttc aaatcgcgca ggcgtttgtc aattgaattc tctattcgga
      961 gatctccaac atccaccgta atgcctgcca caagattggg atctttttcc tgttcgacat
     1021 agagttcggt tccgtgcata tggcgcggta aaacattctc gtcgccgtag gcactgcgaa
     1081 cccgagagcg gattcttcct tcggccttga gtttagtctc gtcataaaat ctaacaacgt
     1141 ccttcacttt cgacgtatct ccattgctaa aaagaaatcg cgcaaatgca tgtaaaatcg
     1201 atttcatctc atgctcactt tttcgatgcg taagcttata aagtgcgagc ccatattgtt
     1261 tggaattcaa tttcatgtgt tcgctttatt tctccaatcc cattaaaact tgccgcgcct
     1321 cttctactaa atattcatga gccttcttgt ccattgcctt ggccacggtc ttttcgacgg
     1381 caatcgctat aagcgatccc atttccctgc ggagaaccgt ctctgcattt tttgtctcgt
     1441 ccgctaattg cttttttccg gcagccacaa tagctgctac ttccttctcc gctgctgcca
     1501 cacgttcggt tcggagtttt gccgctacag cttcagcatt ttttataatt ttttgcgact
     1561 cttcgcgtgc aacgataagg atcgcttctt tttctttttc catgtccgcc accttagact
     1621 ctattttttc cgcttcggcg cggtcagatt ccatgcgcaa aaaacgctta tcgagtatct
     1681 tcatgattgg tccgaccgca aaacgcttaa gcacataaaa aagaatcgcg aagtttataa
     1741 tctgcgctat aagtaccttc cattcaatgc cgagactgtg gacgagctcc cccattttta
     1801 ctttatacga atttaatgat aagagcgact acaagcgcgt aaattgcgat tgcctccgtg
     1861 aaggcgatag ccagaatcat cgcgccttga atctttcctg atgcttcagg gtttcgtcca
     1921 attgcctcca tcgctcgagc cgcaagaaga ccaatgccaa taccggatcc aatggctccc
     1981 aaacctattg ctaatgctgc tgcaatactt tccatttttg tatgtagatt attaataaaa
     2041 ctatttaaaa agacttttaa taaagccgtt cgaaaatact accaaaaagg aggggctttg
     2101 caaacttcta caaatcaatg cgcttcagca tgactttgag tggcacttcc gagaaacagc
     2161 attgctagga ccgaaaaaat gagggcctga ataaagccga caaaaagctc gagtcccaaa
     2221 aaaggtaccg gcgcaagata cggtacaaga gacgcgataa taacgagaag cacttcgccg
     2281 gcaaagacat taccaaacaa tcgaaaggag aatgaaatta ttttagcggc ctcgctcaca
     2341 agctcgagga tccccgcaaa ggcattgccg aaactcgaaa aattaaaaaa cttatgagcg
     2401 tgccctcgaa gtccgatagt cactactccg acaatatgaa caagcgtaac aacaataagc
     2461 gcgagcgcca gcgtcatatt taagtctgaa tataccgatc taaagagcgg cacaaatgtc
     2521 tcaccgcctt catggatctc ggtaaaccct atactgccca cacccggcaa aatgccgatc
     2581 cagttagccg acagaacaaa aataaatatc gtgagagcca gaggaaaaaa cttttttgct
     2641 gtctcgcgat tcggcgcaaa tcccgccata aaattaaaaa tgccttcgaa aaacgcctca
     2701 actgcatttt gaaatctgtc tggcacttcc tttatacgtt tcgatatgac ccaagcaaaa
     2761 atacaaagca gaatgataac aatccagcca gttaatagtg tattggttac cggaaaaccg
     2821 aatatttcga atagatgctc tgactgtatt gaaacgtgga ccatgccaaa tactaatata
     2881 ctaattcata ccaattatac gaatccaatg tatttctctt tattggtatt attggcatac
     2941 attcgtatat tggcatctta ccttaacaca tcctttgttt ttttataaat cagggcaatg
     3001 gaaacgatca tcgatacaaa aatgccaatt agtagtagcc atggcgacgt tccccaccat
     3061 ctgtcgagca gtctacccaa aagcgcaagg agcacaatcg gtatagctat cgtaaatccg
     3121 agttcaagcg caaaattcac aagcttaagc tctttgtatt tgtcttttcc ttgttcatta
     3181 gtttttggtg ttccaatcat taaatcacta cacatcaaac atggggctta gcgttaacgt
     3241 ctttctccaa aatcgaatct tgaatgaata acattatttc agtcttttct cgagtcgcaa
     3301 tactctggtt tcgaggagtt tgaactcatc ccgagttact tgattatgcc ggataacagt
     3361 cagctcgcct ttgacaaatt ctatgttttc ttcaaggcgc acaactcttc cttcaattcg
     3421 atcaactttt ccgtccagtt gatctacctt tccgtcaagg cggtcaattt ttccatccag
     3481 ttgatctacc tttccgtcaa gccgttcaac actacctcga actgcctgca attcctctcc
     3541 aaataattta aaatcacttc ggacatcttc gagcacaatc gtatattgat cccttttgga
     3601 cgatttcttt tctcgcatag atttagtata gcatttttgt gcgtcgggaa ggaatttctc
     3661 tccctacggg agcggtacag gtttcgtatc tcgaagactc gatatactca acctcttcga
     3721 atccttcccg taatgcgtgc acacgcatta ctcctagcac actcacttcg ttcgtgtgcg
     3781 tcgggaagga ttcgaacctt cgtaggcata gccagcgctt ttacagagcg ccctcgttga
     3841 ccacttgagt accgacgcaa tattttgact catagtactt tatcagattt ttgatttcta
     3901 tcaataattt tttgtcatga tagcgaatat ggatgagacc atgcagcatc tttctcccat
     3961 ctattctaaa atcttgacgt atgtatggtt tcaaaaattg agatgtggga atatccgtag
     4021 tttcagccca aaaggaaata atacctttga catcttgatc agcatagcag tagagataaa
     4081 tccgaaatcg cttttcgtct aattcgtatg ttgtccgtag aaacttcaga aacaatttaa
     4141 tcatctgggg gttactatta gcgaaatcaa cccctttccc ttttggactg ttataccctt
     4201 cagcccaata gagcattgcc cccgcgcttt tgagttcatt gtactttttt tgcagtgaac
     4261 gaattctgta ggaaggaatt ttttttgaga accattgatg gttcgcctca tggtggctac
     4321 gtctcttgag atcatattta cgcatgatgt gcacaaccat atcaagcgag acgcgataat
     4381 gtttcgccac atcattcata ctataccctt tttcgtagta tagtttttta attctttcga
     4441 tatgctgatc caaaacaccc atgggtgtat tttatcactc acggctcaaa tgcacaaagt
     4501 ggataagtcg tatgtgctca tagatttgac tgcaaggcaa caaaaactcc gccttcgctc
     4561 agtttttcga tttaatagtt atgcctgcgc ctccgccatt tctggctgct ccgcaagttc
     4621 ttccttctca aagaatgctc ccttctttcc ctttttgacg tcgaacacat attcacttcc
     4681 cttaacattt actgtaattt ctcctccttt catgatacct tgcgagatca taagtgacgc
     4741 cacaggattc aagattttat tctgaataat acgctttagc ggccgagcac cgtactgggg
     4801 gtcatagcct tcgcgagcaa gctgactaag tgcaccgtct cctacaatga gggtaatctc
     4861 cttttgtgcc agtcgctctt tcacaatatt gacctggatt ttcacaattt cacttatggc
     4921 ttcaggagac aaaatatcaa agactataat atcatcgagg cggttaagaa actccggtcg
     4981 gaagtaatct ttcaagctct cgagcacttt atccttgacc ccctcgtagt tgctttttgc
     5041 gtttctttca tttgcaaaac ccattttctc catacgatcg atgtactgcg caccgatatt
     5101 tgacgtcaga attattattg agtttttaaa attaacaacg cgtcccttat tatccgtcag
     5161 gcggccattg tcgaaaatct gcaaaagcac gttgaacact tcgggatgtg ctttttcaat
     5221 ttcgtcgaaa agtatcacag aatatgggcg atgtcggagc tgttcagtaa acgttccgcc
     5281 ctcgtcgtag ccgacgtatc cggggggtga gccgaccagt ttggaaaccg aatgtttctc
     5341 catatactcc gacatatcga tccgaataag ggcccgctca tcatcgaaca taaattcagc
     5401 gagtgctttt gtcagttctg tcttgccgac tccggtaggg ccaagaaaaa tgaatgatcc
     5461 gattggccga tttggatcgg caataccggc gcgagagcgc tttacggtat ctgcaatttt
     5521 tttcaccgct tcattttggc cgacaattct ctttgtcagg aattcctcca ttcgtccgag
     5581 cttcagcgcc tcttcctcaa gcattctatt gaccggaata cctgtccaac gcgaaacaac
     5641 gtcagcaata tcgttttctg taatttcctc cttcagtatt cttcgtgaac tttgaagacg
     5701 cttcaaacgc ttgtgtttac tttcgagttc cttctcgaga ttcggaattc gcgcgtatcg
     5761 aatttcggcc gctcgggcaa ggtccgccct tgattctgcg tgctcggcat caatgcggag
     5821 cttctcaaga tcttttttaa tcgacttaat gccgccgata atttctttct cgtttttcca
     5881 tttcaattcg agttctgaag ttttttcctt taaatctgcg atctcttttt ctatcttttt
     5941 gacgcgcaat tttgctgttt tcgaatttgg atcattcttg agcgcttctc gttcaatctc
     6001 aagacgcata accttgcgac gtgtctcttc gagaacaggg ggcatgtttt caagcgaaat
     6061 tttaagctgt gaggccgcct catcaatgag atcaaccgcc ttgtccggca agaatcggtc
     6121 cgtaatatat cgcgagctta ggttgaccgc cgccacaatg gagtcatccg taattttgac
     6181 accgtgatag agttcatatt tttctttaag ccctcgcaat attgccattg cgtcttcaac
     6241 ggatggttcg ctgacaaata ctggctgaaa tcgtcgagtt aaggcgggat ctttttcaat
     6301 atgcttttga tattccttaa gtgtcgtggc gcctatagca cgaagctcac ctctcgcaag
     6361 ggcaggtttc agcatgtttg atgcatccat agaaccctct gcggcgccag caccgacaat
     6421 cgtgtgaatc tcgtcgataa aaagaataat cttgccttcg gagcgttcaa cttctttcat
     6481 tatgttctta agccgctctt caaattcgcc gcgatatttc gttcccgcga ttaagaggcc
     6541 gagatcaagc gagacaagct ctttatcttt aagcgattct ggaacatcac caagagacat
     6601 ccgaagcgcc agcccttccg caattgcagt ctttcctaca ccagcttctc cgattaagat
     6661 cggattgttc ttcgttcgac gcgaaagtat ttgaataatt cgcgaaattt ccgtatctcg
     6721 gccaataacc ggatccaatt tattttcgcc ggcaagtttc gtcagactac gcgtatattt
     6781 agaaagaaat ttatatttct taggctggtg tacgtccgtc accttgctgt tccgaagttc
     6841 ttcaagtaca cgcataacgc cgtctttctc tatgcgaaag cgtgtcaaga tatctcgcgc
     6901 attaccgggc acatcaaaaa gcgcaatgaa caagtgttca gttgacacaa attcatcttt
     6961 cagcgcggct gcaactttgc cagatccttc tattgcctgc gccagttcag gagttaaata
     7021 cagctggtac gaaggagaaa ggacactccc tgattccgga ctctcgatgg cttcgagcaa
     7081 agaatcggta agtaaaatag tatcgacttc aagtttgtcg aggatagagg taaccatgga
     7141 ttcctcctga aggataaggg cgccaagaag atgaagcgga tttacgtggt tctggccacg
     7201 ctcaattgca agttcgtgcg cgcgcctgat cgattctttg gccttcgttg taaaatgatt
     7261 gaatggtgtc attggaatag cttatagctg tttccgcccg aagcggatcc gcctttggcg
     7321 gaagcgtata gggtttagcg tatagttcat aaggggttta actagcatat ataattaatt
     7381 gtacaggatt atcgtcactt gcagtagacg aatgatttag gttcccgtta caaaatatta
     7441 tatagagatt actatacata gtcaaatctc tatatgtcac tggtttaaga gctataaata
     7501 atgctacttc tctgatttca actcttcttt gatgttggag agatctgatc tgattgattc
     7561 tatggaaata aacccttggc cattcaaaat atctgatttt tttgttccga gcccgacaag
     7621 ttctccgctc aatgtaaaca acccgagacc atgcgacgtt tcatcactaa tgtcagtgtg
     7681 aatgatgggc gttgcctctt tctcttctag tgccgttaca atgccacgaa atacagcaag
     7741 tttgtcctcc acattgagaa taaatacatt ttgccccaaa agcacttctc ctttcttaaa
     7801 tagcaccgga acaagctctg ccctcgtgac aagcgaggga tcagccttta gatatacaat
     7861 ctgctttata tcatttcggg caacaatcga aagcggaaaa ttggaatttt ttgtgacggc
     7921 aataagacca gactcttctt tcaaaactcc catatcagcc acaaaaattc cctgggcgtc
     7981 gagcaacagc cctctgctag cagtgctgtc cgttccttct ttacgtatca gtacgaaatt
     8041 ttttgaaacg gaagccacag cagttgcgag cgcttcgtcg ggaggcacgg gtatttttat
     8101 ttctttagtc accgttgcaa tcgactctga cggaacaaca cgctcgattg tcttctcgaa
     8161 aatttgatga atcgtctgcg acacgcccgc gggagcttgg tcaacaaggg aaaccgtaac
     8221 gacgccagta gcaatcgagg ttacaaaaga aaccaaaagt gcgagcaaaa taagttgttg
     8281 tttagttaaa tcctccatcc atggggtaaa aaaaattcca tcactcttta gactcaagag
     8341 agcaatcagt tgtattgtat ctgatttcca aacacttagc ccagttagac ttatcaacag
     8401 gctacataca aaaagtctgc ctcaatgctt tttttctacc cgacctatat tgtatcagcc
     8461 aaaaacttta gccttcttgg taaagattgg ttggccttat tcgaattaaa tcttgtttga
     8521 cccacccatc agcgcctcta tcaaaattta cgtagcaaaa ccaaacattc tcatcaaaat
     8581 taaacgttgg atttaaaggc attgagacaa cgacacctaa tgtgatactt gattgaggat
     8641 tcggagacgg caccggcgtc accgagagag acgtcgattc aggcgttgcg tacacaaacg
     8701 cttgagaaga agcccgaacg cgatatccag ctaaacaatt cgggggtttt tgctgggact
     8761 gtgaacttgc cgatgaaaaa tttgccgtta catttttatc agacgtcata gacacggtac
     8821 acgatccagt tcctgaacac gcccctgtcc ctgtccaact ggaaaagacc gaacctgaag
     8881 tagggtgcgg agtgaggaca acttggtcat tctcaagata gacttgggtg caattaggtt
     8941 cacatgaaat tcctcctgtt cccgatacag ttccgcctcc aacaacgacg acagtaagcg
     9001 tgtaagaggg cacatcgata taaaattcaa tttgctccgc tggatcattt gcataggcat
     9061 ttctgatatc aggtatatag ggtaatagcg tcgcaattga atcctgtgct tctggagtcg
     9121 catctttaca tcgcacgaag taacttctaa aatcgccatt tgatagtccc gagatatttt
     9181 ctgtatgcgt ggtatccaaa gttgatccaa aagtatatgc catagatgtg tatgacacgt
     9241 cggccgtatc atatttacac gtcgagacgc gatctgtcgt tactgtcaga tctacatctg
     9301 tagtacctac aggttgagat cctgcaggtc cttttgacag aatctgtgaa gcggaagcaa
     9361 aatgggcttc caaattggcg gccgcttcta cagaaatggt gcattcttct tcggccgttt
     9421 cgcacgctcc agaccatccc gcaaacgtat acccgctaaa cggatcggga gacgccgtga
     9481 atgttacgag cgaccctgct gcatatgtac ccgtacaagt aaacactcca cacgtgagcc
     9541 ccggagcaga tattgctcct gatccacttt ttgtgaccgt gacaacatac tcgacgacat
     9601 cgtatgtcac attaaaatta atccagccaa caatatcgga gccccatgca tatccgatga
     9661 gtgtcgtatc tgaagctttg tacgtaacgc caccacctcc agtagcatcc ggtgaactat
     9721 ggttcgtgcc cgaaagatga atccatccat cccaaaaatt cgatccagaa accggtgaga
     9781 gcgcccgagc ccacccgttt accgcaccgg agccaaagtt aacttttgca atagagccag
     9841 tgccagaatt aatatcagaa ttcggaggat tgccagtctc ggcgcgatca aagctaatcc
     9901 aacctatagt ttcagcccat atataaccgg aaatattgcc cgacaaatca agcgtagcac
     9961 cataattaga cacggaagat cctgcggccg gacaccctgc gtgtgcagat gcttccgttg
    10021 aagacataat cccgtttcca tccgtgtcac aatcaatgct gttaaaacta atccaaccta
    10081 tcgttccgga ccatccgaag ccacttaagt tatgctctgt accagcagag acactcgacg
    10141 ttactgcaaa aagacttgcc gctacaaaaa caccaatata gaatgattta acgttaacac
    10201 tcacgagtct gtgcttgtta cctttcatat caaacaagaa tggcgagttt ttaataatcg
    10261 cagatattta gaatgtaatt atctccgtca tcagcatcat catcataagt taaatatgat
    10321 gcgggaacag cctctgtaac tgtcgggctc cagccggagt ctggatcgtc ccaaagttga
    10381 aatctattgc cactttccct agtatcaaac tgaagtattt ttacatccgc actgcttccc
    10441 gtatcgccat ttctaccctg tatatttgtg ttacctccag aattatctgt gtagagccac
    10501 ctgttatttt cttcggactg tttaaaatat tgcggactgc ttccgtagta attgaaacca
    10561 ggaacatctg caccggaatt tgaagtgaga ttgtatgccc agacatggat tgagcaaccg
    10621 atgttatcgg cgcatatatt tcttccgttg atgaccatat tgatcgttcc cctgccttgc
    10681 gagtcgccat tggcagtatc aatagtgacg cacgtatttc caccaccgct aatatttgag
    10741 agagcctgcc acgtcgcaac gccgttggcg tccgatttta gaaccttgtc cgcaccctga
    10801 ctgccgtcaa caatttttat tttccccaaa acatcgagct tgatggaatt tgaggtatct
    10861 ggggacgcca aaccgagccc aaaagttccc aatttccctt gcggagccga tgatacattt
    10921 atgggcatgg ccacatttcc agtgggagga gttgtcgttg gagcgttcca tgaatagatg
    10981 aggttcgctc cgagcgtcaa cacaagcgcg atcactgtta ttcgaactgc agagaaaaaa
    11041 ttttgtctca atgttttcat atgaattaga ttaccttaga agaaattata gcattttaaa
    11101 aggctcttta ttgacaaaaa aatagtaccc tgtgaataac aaaatacgct gttttacctt
    11161 atgttttctt cgtagactca aaaatacttg agatcaaata acaacttatc tttcttcgta
    11221 ggatgtctgc ttgcgaattg gcattatatc ctcatcaacc cacccatcag gacctttatc
    11281 gaaatctata aaaca
//
LOCUS       DATDSU010000057         5789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1227688, whole genome shotgun sequence.
ACCESSION   DATDSU010000057 DATDSU010000000
VERSION     DATDSU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227688"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..569)
                     /locus_tag="VI981_01930"
     CDS             complement(333..569)
                     /locus_tag="VI981_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479092.1"
                     /translation="MTISILFVYRIPAPFASTDIDVVIIALTTALLEIYLIKKQPPFG
                     DCFFEIKLDYRRRFVDFLAFRFAAFRFFAIEIEM"
     gene            562..1392
                     /locus_tag="VI981_01935"
     CDS             562..1392
                     /locus_tag="VI981_01935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY4479093.1"
                     /translation="MVIFRRYSLYAAIFIALFWVAYVSLSLLSERSSYLGRLILETEA
                     FADLPEFLGQTSTGTAVAAVLPAVVRVVAYRDEKLFDVQVIDGQVKAEQVGKTRKNVR
                     TGTAFFISDDGYLITNKHVVADDRALYVVHTGKDELPANVVYRDPKYDLSILKVEGSA
                     FPTISFSDTSPTEGDVVATVGNAFGRVIDSTSQGKILGLNRKIVADGGLRGEELLDGL
                     IESDALLFSGDSGGPLLNVKGEAVGVNVAISTDEARSSFSITAKIAKEVIARAGIFVN
                     "
     gene            1732..2661
                     /gene="gnd"
                     /locus_tag="VI981_01940"
     CDS             1732..2661
                     /gene="gnd"
                     /locus_tag="VI981_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY4479094.1"
                     /translation="MAKNNSNSIAIFGLGRMGSQIARRLHKKKFNVVAWNRSPEPAKE
                     VAKSGVYATSNISEAITKMSKSGRIFWVMLPHGIVDEFLFGKDHLGPHLKKGDIIIDG
                     GNSFYKDSMARAEKLVAKGVHFFDCGTSEGVWGEKNGFALMVGGDKEKWPIIEPIMKA
                     LSSGDNFSYLGKAGSGHFTKMVHNGIEYGMMEAIGEGYAVLKASPFKLNLRDVTRVYR
                     EGSVVRSWLIDLVRNIFEHENVEKTIGKIDATGEGQWTVEAGEEFGVDVRVLEDALRV
                     REESKDPKNQNKFSNKIVALLRKQFGGHAVQGK"
     gene            complement(2865..3773)
                     /locus_tag="VI981_01945"
     CDS             complement(2865..3773)
                     /locus_tag="VI981_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY4479095.1"
                     /translation="MSKLCLEFWVHWLEFGAMVDNINIRKVRNALKLAKQHGVAGIEF
                     PWKPLMALEPEVLAKLMLAAGIKKFALCVFYGRGGHDPLRRRDQPRVLKDLGAAVNFG
                     YTLASYGLEFVGITGPWGFQIEKTYRKSAALRTNIQSLLMDVADLAIDTSTMFALEWL
                     QSAENKAINDPRDLFGLLNNVDCHYVGAHLDSFHFDAEQIPQKAILEAAGKHLLWYHV
                     SGHQRFTPGGVGDTIEWPLVADGLSKNIELGGSLKAVCFEGFSRDFRKLVPEIGRTYP
                     QDVPAQVGLPLCRKTLEDAGIIGKAT"
     gene            3944..5464
                     /locus_tag="VI981_01950"
     CDS             3944..5464
                     /locus_tag="VI981_01950"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY4479096.1"
                     /translation="MQKERYEKEISELKLKLGEISHLGSTLALLNWDQEVNMPRKAGA
                     SRAQAISYLSGVIHNKFLDLNKKSVLGTLKEAVDKKKIRGNNAIIVVELWRSYERERK
                     LTDSHVRALAELASQAHTAWVKARHENKFSIFLPYLEKLVELKRKEAKLIGYQDSPYD
                     ALLDGFEPGLTAKETSRILNELKAFLVLFLAKVRRLNLKSQKNKIHGKFPILEQRKFS
                     ELIMTKMGFDPEAGRIDESAHPFTSGFHPHDVRFTTRYYEHNVLESITCTIHETGHAL
                     YDQGLPVEHFGTPLAEATSLAIHESQSRLWEIFIGKSSPFWRYFYPKLQKFFPKPFKN
                     IPVSEFNKVLNEINPAFIRTESDEVTYNLHIILRFEIEKMLIEGQIEAKDLPEIWNSK
                     MKSYLGLDVLSDSVGVLQDVHWSHGYFGYFPCYTLGNLYGAQFYYTMRKQIKDIDKKM
                     VKGEFGEIREWLRAHIHVHGKTYMAAEIVKRVTGEKLNPKYFIQYVALKYSKLYKI"
     gene            5484..>5789
                     /locus_tag="VI981_01955"
     CDS             5484..>5789
                     /locus_tag="VI981_01955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4479097.1"
                     /translation="MKLVFVRHGRTKSNVESRTMGRRIDDSLLDEGKAEAKAARELLR
                     HNFSRIYTSPQKRAFDTAKIINERLRLPMEVRSEIVERDFGTLSGILWKEMSDKTDGW
                     "
BASE COUNT         1655 a         1171 c         1438 g         1525 t
ORIGIN      
        1 ctggcggctc actacggggc gagaagtggt tacgtactaa aaatatttag cggtgcgttc
       61 agttgtagct tcaataatca tgtcaagact tgtccacccc agtagtagat ccgccaactg
      121 gcggctcact acggggcgag aagtggttac gtactaaaaa tatttagcgg tgcgttcagt
      181 tgtagcttca ataatcatgt caagacttgt ccacaggtgt gagttcaaat tctattgtat
      241 taattttctg gcggttcata attagtgcat agtcaatgca aactttttgg tcgaaatttt
      301 ttgtatactt tttagaaagt taacacagta atttacatct caatctcaat ggcaaagaaa
      361 cgaaaagccg caaagcgaaa ggctaagaaa tcgacaaagc gacgacgata gtcaagtttg
      421 atttcgaaaa aacaatcgcc gaaagggggt tgttttttta ttagataaat ctcaagaagt
      481 gcggttgtca gtgcgataat tacaacatca atatctgtgc ttgcaaaggg ggccggaata
      541 cggtatacaa aaaggatgct tatggtcata tttcgacgct atagccttta tgcggccata
      601 tttatagcct tattttgggt tgcctacgtt tctttgtctc tactttccga acgttcatca
      661 tatttaggca ggcttatttt agagacagaa gcgtttgccg atttgcccga attcctcggt
      721 caaacgtcga ccggcaccgc ggtcgccgcg gtgcttcctg ccgtggtgcg ggtcgtcgcc
      781 tatcgggacg agaaactttt tgatgttcag gttattgacg gacaagttaa agcggagcaa
      841 gtcggtaaaa cgagaaaaaa tgtacgaaca ggtaccgcgt tttttatttc ggacgacgga
      901 tatctcatta caaataaaca cgttgtcgct gatgaccgtg ctctctatgt agtacacacc
      961 ggaaaagatg aattgcctgc aaacgtagtg tatcgcgatc cgaagtatga tctctccatt
     1021 cttaaagtgg aaggaagtgc gtttccgaca attagttttt cagacacatc tccaactgaa
     1081 ggtgatgtcg tcgcgacagt cggcaatgcg ttcggccgag ttatcgattc gacaagccag
     1141 ggaaagatac tcgggctgaa ccgcaagatc gtggctgacg gcggtttacg cggagaggaa
     1201 cttctcgatg gtctcattga gtccgatgct ctgctttttt ctggtgactc gggtggcccg
     1261 ctcttgaatg tgaaaggcga agcagtcggt gtaaacgttg caatctcgac agatgaagcg
     1321 cgctcaagtt tttccattac ggcgaaaatc gccaaagaag tgatcgcgcg cgccggtatt
     1381 tttgtcaatt aggagatttt agctttcatt tgcctactcg atgaggttca tgatgtaaat
     1441 tgtcgatcct acttttctct gtttgcgagt gaccctttgg aaatggggct ggattcagga
     1501 aaaaacaata gatcacaaca ttggtcaaga ggtcaaagtg gcgctttata gagctacaga
     1561 gtatgcttgt taagctagat agcggtcgag tttggtttaa tattagttaa actcaaaaaa
     1621 acatggcaga cgacccaaaa aaaccacagc cgccagaacc agctgatggc acgactacta
     1681 aataactaaa agccccaaca ggggctttta gttttgtgtt aatatttttc tatggcaaaa
     1741 aataattcaa actcgattgc gatatttgga cttggccgca tgggaagcca gatagccaga
     1801 agactccata aaaaaaagtt taacgtcgtt gcgtggaatc gctcgcccga gccggcgaag
     1861 gaagtggcga aatctggagt ttatgcgaca agtaacattt ctgaagcgat cacgaaaatg
     1921 tcgaaatcag gcagaatttt ttgggtcatg ctgccgcatg gcattgttga cgaattttta
     1981 ttcggtaaag accatctggg tccgcatctc aaaaaaggag atatcattat cgacggcgga
     2041 aattcttttt ataaagacag catggctcgg gcagaaaaat tggtggcaaa gggagtccac
     2101 ttttttgatt gcggcacatc ggaaggtgtg tggggagaaa aaaacggttt tgcgcttatg
     2161 gtggggggtg acaaggaaaa gtggccaatc atagagccga ttatgaaagc actctcctcc
     2221 ggagataatt tttcttattt agggaaagcg ggttcagggc attttacaaa aatggtccat
     2281 aatggaattg agtacggaat gatggaggca atcggggaag ggtacgcagt acttaaagca
     2341 agcccattta agttgaattt aagagatgtg acgcgcgtct atcgagaagg atcggttgtt
     2401 cgtagttggc ttatcgatct tgtgcgaaat atttttgaac acgagaatgt ggagaaaacc
     2461 ataggaaaaa tcgacgcgac cggagaagga caatggactg tagaggcggg ggaagaattc
     2521 ggggtggacg tacgagtact tgaagatgca cttcgcgtcc gcgaagaatc aaaggaccca
     2581 aaaaaccaaa ataaattttc aaataaaatc gtggcgcttc ttcgcaagca atttggcggc
     2641 catgcagtgc aaggaaaata atatgaacaa aagacatgag agacggcaac tgcacgtaac
     2701 gtatctcatt aaattacaat tgggattata caacgctata tagcgtaata taatcgcact
     2761 ttagtgttca ctattcccac tattatttat atttctttac gtacttgtgt tgtgacgcct
     2821 taaaatcgta tccatgtttt ggtgccgtta aaaaaaagcc gccgttaggt agctttccca
     2881 atgattccag cgtcctcgag cgttttcctg caaagaggaa ggcctacttg ggctggcacg
     2941 tcttgggggt acgttctccc gatttcggga acaagttttc tgaaatccct gctgaagccc
     3001 tcgaaacata cggccttcag gcttcctccg agttcgatgt tcttggagag accgtctgcc
     3061 accaaaggcc attcgatcgt gtcgccaact ccccctggtg tgaagcgctg gtggcctgaa
     3121 acgtggtacc aaagcagatg tttaccggcc gcttcaagaa ttgccttctg aggaatttgc
     3181 tcggcgtcga aatggaaact gtcgaggtga gctccgacat agtgacaatc gacgttattc
     3241 aacaagccga acaagtcacg cgggtcgttg atggccttgt tttcagcact ctgaagccat
     3301 tcaagggcaa acatcgtgct cgtgtcgatg gcgagatcgg cgacatccat gagcagtgac
     3361 tgtatgttgg tccttagtgc cgcactcttt cggtacgttt tttcgatctg gaagccccat
     3421 gggccggtga tcccgacgaa ctcgagaccg tacgaagcca aagtgtatcc gaaattgact
     3481 gcggctccga gatctttgag tacgcggggt tggtcgcgcc ggcgcagtgg atcatgtccg
     3541 cctctaccgt aaaagacgca gagtgcgaat ttcttgatgc ctgcggcgag catcagtttg
     3601 gcaagcacct cgggctcgag tgccatgagt ggcttccacg gaaattcgat gcctgctacg
     3661 ccgtgttgtt tggcgagctt gagtgcgttt ctcactttgc ggatgttgat gttatccacc
     3721 atggctccaa attcaagcca gtgaacccag aattcaagac agagtttgct cataagaaga
     3781 agtgccgtag ccgatttgaa cagtttcgtc ctttgtcctg caatcttgca gagggcgaaa
     3841 taccagtgtc aacaatatag cgaaagctgt ctttgtcaat gatttttttt agcgctatgc
     3901 gtatgaatta aaatatggtt aactgtactt aagaacatac gaaatgcaga aagaaagata
     3961 cgaaaaagaa atcagcgaac taaaattaaa actcggcgag attagtcatc ttggctctac
     4021 gcttgctctc ttaaactggg atcaggaagt aaatatgccg cggaaagccg gagcgtcacg
     4081 cgcgcaagct atttcatatt tgagcggcgt tattcataat aaatttctcg accttaacaa
     4141 aaagagcgtt ctcggaacgt taaaagaggc agtcgataaa aaaaagatca ggggtaacaa
     4201 tgcaattatt gttgttgagc tctggcgctc atatgaacgc gagaggaaat taacggactc
     4261 gcatgtccgt gcgttggccg aactcgcatc gcaagcgcat acagcatggg taaaagcgag
     4321 acacgaaaat aaattttcga ttttccttcc gtatctagaa aaattggtgg aactgaaacg
     4381 taaagaagcc aagttgatcg gctaccaaga ttctccgtac gatgcgcttt tggacggttt
     4441 tgaacccgga ctgacggcca aggagacgtc gcgcatacta aacgagctca aagcgtttct
     4501 cgttcttttt cttgctaaag tcagacgact caatttgaaa tcgcagaaaa ataaaattca
     4561 cggaaagttt ccgatcttgg aacagcgaaa atttagcgag cttattatga caaaaatggg
     4621 cttcgaccct gaagcgggca gaattgacga gagtgcgcat ccgtttacgt ctggttttca
     4681 cccgcacgat gtgcgattta cgactcgtta ctacgaacac aatgttcttg aatcaattac
     4741 ctgcacaatt cacgagacgg gtcacgcgct ttacgatcaa ggattgcctg ttgaacattt
     4801 tggcacgccc ttggcagagg cgacttcgct tgcgattcat gagtctcagt cgcgactatg
     4861 ggagatattc ataggaaaaa gttctccatt ttggagatat ttttatccga aattgcaaaa
     4921 attctttccg aaaccgttca agaacattcc cgtgtcggag ttcaataaag tgctgaacga
     4981 aatcaaccct gcatttatcc gaacggagtc tgatgaagta acttacaatc ttcacattat
     5041 tttgcgattt gaaattgaaa agatgttgat tgagggccag attgaagcga aagatttgcc
     5101 tgaaatttgg aattcaaaaa tgaagtcata tcttggcctt gatgtgctgt ctgacagcgt
     5161 gggtgtgctt caagacgtgc actggtctca tgggtacttt ggatattttc cgtgctacac
     5221 actcggcaat ctttatggtg cacagtttta ctacacgatg cgaaaacaaa taaaagatat
     5281 tgataaaaaa atggtgaaag gagaattcgg cgaaatcaga gaatggttgc gggcgcatat
     5341 tcatgttcac ggcaaaacgt atatggcggc agaaatagtg aagcgcgtta cgggcgagaa
     5401 acttaatcca aagtatttta tacaatatgt cgctttgaaa tattctaagc tctataaaat
     5461 atagtgaact ttaaagcgag aaaatgaaac tggtctttgt gaggcacggg aggacaaaat
     5521 caaatgtcga aagtagaacg atgggtcgcc gtattgatga ttcactgctc gatgaaggaa
     5581 aagcggaggc taaagcggct cgcgagctgt tgcgacataa tttctcacga atatacacct
     5641 cgccgcagaa gcgagctttt gatacggcaa aaataatcaa cgagcgtcta agacttccga
     5701 tggaggtacg aagcgagata gtggagcgtg attttggaac tctctcgggg attttgtgga
     5761 aggaaatgtc tgacaaaaca gacggctgg
//
LOCUS       DATDSU010000058        15177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_220210, whole genome shotgun sequence.
ACCESSION   DATDSU010000058 DATDSU010000000
VERSION     DATDSU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15177
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220210"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..432)
                     /locus_tag="VI981_01960"
                     /pseudo
     CDS             complement(1..432)
                     /locus_tag="VI981_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052596.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
     gene            complement(564..875)
                     /locus_tag="VI981_01965"
     CDS             complement(564..875)
                     /locus_tag="VI981_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479098.1"
                     /translation="MLVESPLKRIVEKIFRTETGEFIRATFLVVEFHGQIRGKIIKTE
                     IISPDTFTANQEVLSLSEPYSPQVANFAPYIYHEPKVSPYFEFNFLVSQPTRAPARRS
                     G"
     gene            1009..1254
                     /locus_tag="VI981_01970"
     CDS             1009..1254
                     /locus_tag="VI981_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479099.1"
                     /translation="MIYGNISFLNVKTKRQRILARILGTITSTLMSIFFGCGMFMLFR
                     QEHASPLVAGIAGVAVFALTFWFLVIVLVWKQTRAPK"
     gene            1566..1796
                     /locus_tag="VI981_01975"
     CDS             1566..1796
                     /locus_tag="VI981_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479100.1"
                     /translation="MNIEIGTIVRSGTKKALLEICGFTTDGKYLARKVRVVIKESALL
                     KYFKLEMLQPGSTLLLPNGLKIRLTLKSTAST"
     gene            complement(2010..2654)
                     /locus_tag="VI981_01980"
     CDS             complement(2010..2654)
                     /locus_tag="VI981_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY4479101.1"
                     /translation="MNDDVNNDPADVVPTDEEGNPLKVKRSDLKSEKRSDLDPDGLIE
                     EDNDLKSQVTKLKEKLKTALEEKQKYLDGWQRDKAEFLNARKRDREAQAEFLKFAKED
                     IVTELLPVLQSFEMAMANKEAWEKVDKNWRIGVEYISNQLKTVLETHGLKDVNPIGLQ
                     FDPMRDEAQEFVPVENEEQNNKVMEVIQKGYELNGKQLRAPKVKVGEFKKADSE"
     gene            complement(2750..3160)
                     /gene="rpsI"
                     /locus_tag="VI981_01985"
     CDS             complement(2750..3160)
                     /gene="rpsI"
                     /locus_tag="VI981_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY4479102.1"
                     /translation="MQPTKEKTIYIETIGRRKTSTARVRITVSPKQSLLINEKGLEAY
                     FPTVHLRSLVLEPISKLKITEKFKITALLKGGGINSQAEALRHGISRALVVHNGELRK
                     QMKKAGFLKRDPRAKERRKFGLKKARKAPQWSKR"
     gene            complement(3163..3513)
                     /gene="rplM"
                     /locus_tag="VI981_01990"
     CDS             complement(3163..3513)
                     /gene="rplM"
                     /locus_tag="VI981_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY4479103.1"
                     /translation="MMKYTIDAQNKRLGRVASEAASLLMGKNKTNFVRNANPKDIVVT
                     ISNCSKIVLPRKKMRDTKYARYSGYPGGLRFLSMEQIAQKKGYSELFKIAVRGMLPGN
                     KLRPDMLKRLIVTE"
     gene            complement(3603..3953)
                     /gene="rplQ"
                     /locus_tag="VI981_01995"
     CDS             complement(3603..3953)
                     /gene="rplQ"
                     /locus_tag="VI981_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY4479104.1"
                     /translation="MRHHKKNRVLGREAGLRKALLRSLASSLILHGKIETTEAKAKEL
                     RPFIEKLITKGKNDTLSSRRLLIARLGSVRLVSKVISDISTKHKTRQGGYTRITKMGR
                     RASDGAKLAQIEFV"
     gene            complement(4061..5035)
                     /locus_tag="VI981_02000"
     CDS             complement(4061..5035)
                     /locus_tag="VI981_02000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY4479105.1"
                     /translation="MSEHNVVLPSKPKIIHEEKMSGVYEIDGLYPGYGHTLGNSLRRI
                     ILSSLPGAAVTRVKIEGVNHEFSTLPGVKEDVITILLNLKRLRIKMLTDEDQILTLKI
                     KGVKDVTAADITAPGQVEILNPELRIATLTDKGAELSMEMTVEKGLGYVAKEQLQKDR
                     VDIGSITLDSVFTPIRRVNYEVENMRVGNRTDFNRVKIFIETDGTLTPKEALEKSIEI
                     MINQLKAIVGFKEEEPEVKLVEPTPLAKAESPIAAVKEIDPEFLKTRIETIGFSPRTL
                     NALTNANIRTLGGLARKKEEDILDIDGLGSKGVQEIKKVLAGYGITLK"
     gene            complement(5061..5663)
                     /gene="rpsD"
                     /locus_tag="VI981_02005"
     CDS             complement(5061..5663)
                     /gene="rpsD"
                     /locus_tag="VI981_02005"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY4479106.1"
                     /translation="MKIGPRYKIARRLGAGVFDKTQSAKFQARAARKTRIISRPKSDF
                     GIQLLEKQKARFTYGVGERQFSRYVKNAIAQKKQKAADELYRTLESRLDNVVYRMGIV
                     VSRAAARQMVSHGHITVNGKKVSIASFKVSPKDKVAIREASKNKALFQNLDEKLKEVR
                     TPNWIKFNPETKVAEIATPPKREGSETFFDINAIVEFYKR"
     gene            complement(5844..6275)
                     /gene="rpsK"
                     /locus_tag="VI981_02010"
     CDS             complement(5844..6275)
                     /gene="rpsK"
                     /locus_tag="VI981_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY4479107.1"
                     /translation="MGKKRIITKGETGEGEAGKSGGGKSAKKKLDRGVLYVQSTYNNT
                     ILTLTDKQGGVVGAASSGALGFKGAKKGTPFAAAKVGETLGTKAQAMGLKEIDVVIKG
                     VGSGRESSVRGFLSKGINLLSIRDATPVPFNGPRPKKPRRV"
     gene            complement(6410..6793)
                     /gene="rpsM"
                     /locus_tag="VI981_02015"
     CDS             complement(6410..6793)
                     /gene="rpsM"
                     /locus_tag="VI981_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY4479108.1"
                     /translation="MMRISGITLPENKRMEIALTAIFGIGRTRAQKILTEAKVEFGKR
                     PKEISPVEENEIRKIVETYKVEGDLKREIGGNIKRLKDVKCYRGVRHLRKLPVNAQRT
                     KTNSRTVRGNVRKTMGSGKKAVDKK"
     gene            complement(6946..7062)
                     /gene="rpmJ"
                     /locus_tag="VI981_02020"
     CDS             complement(6946..7062)
                     /gene="rpmJ"
                     /locus_tag="VI981_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY4479109.1"
                     /translation="MHVKASVKKICAQCRVVRRKGHLYVTCKSNPRHKQRQG"
     gene            complement(7130..7345)
                     /gene="infA"
                     /locus_tag="VI981_02025"
     CDS             complement(7130..7345)
                     /gene="infA"
                     /locus_tag="VI981_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004691460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY4479110.1"
                     /translation="MIKKEVATVSGVVTEALPNTFFRVKLEGQDEVMLAYLAGKMRMN
                     RIRVLIGDKVLVELDPYGGKGRIIKRL"
     gene            complement(7518..8807)
                     /locus_tag="VI981_02030"
     CDS             complement(7518..8807)
                     /locus_tag="VI981_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479111.1"
                     /translation="MAKNILEDVLPPERRSIRNIHLAGKRVRVERPERREEPSTLVEL
                     EPKKRSPKRRSRVLLFIGFFVVLVIIFLTFSAFFTGATVSVEAKRQIINVDSTFQSKK
                     GATADLFHEIVMLEKDGSKKVEGTEEKDIEKKASGKIVVYNNFSTESQRLIKNTRFMS
                     PTGLIFRISESIVIPGLKRTNGSATPGSIEVTVYADQAGKEYNIGLVDWTIPGFKGDG
                     QKYETIYARSKTTMSGGLKGKTRIIDDQTRSNTQKEIEGSLKTEMLREVRSNLPSDYV
                     LYDKAVYYEFEKGIEDSVSGNQVEVKEKGRIYGIIIRKDSFVAALAKKYIKDYKDAKL
                     HFENPETLDVRIIDDPQVKPWAANELRVAVVGGAALVWDFDEALLKDALAGKPKKSLE
                     GILDAFPAVQGADVILRPFWKMHFPSDKDKIEIIAKK"
     gene            complement(8857..10047)
                     /locus_tag="VI981_02035"
     CDS             complement(8857..10047)
                     /locus_tag="VI981_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479112.1"
                     /translation="MSFFSKKAHEEIGMVFHINSADVRGAIVGFTQGGTPMLHRAHTE
                     IMAPIAESSRASFIHEMVKALNTCATALVKESHVLERVSSKSIKIRKVFCVLSSPWFL
                     STTKIVTSVKPLPFTVDEIFVGRLVEDEKRLFLDKIKTADTSDETVLEAKIVTIKLNG
                     YEISKPYGQRASSVEVMLYLSASPKSLMESIESTIHKHFTFKAFECHTMPLVTFSTMR
                     NLEGLNDQYLVVEVGGEVTDIMLVKRGLLVETLSIPIGKNAFLLQSAKALKTIPQVTR
                     SFIKARAEGKMDKAFSEKLKKSLDIAEMKWVSLFEEVLTKVSGEAFIPPKVFLVADDD
                     IALYFKDVIGRAGFVQADSLRQEFEVVLLDHAVIAKNLSQAPNVKEDVILALDSIFVN
                     HIYS"
     gene            complement(10126..10449)
                     /locus_tag="VI981_02040"
     CDS             complement(10126..10449)
                     /locus_tag="VI981_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479113.1"
                     /translation="MNNLANIGLQRATVKLQALVKSRSALGGDFSIKNHGHTELELRV
                     RIVAYEVHSRFIDKKFMGSLDEHVEALALQVLEQAEQSLSRGYERDQVLRVLRDSKAV
                     SLQEL"
     gene            complement(10808..12616)
                     /locus_tag="VI981_02045"
     CDS             complement(10808..12616)
                     /locus_tag="VI981_02045"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY4479114.1"
                     /translation="MKLAEVRTQYLEFWKSKEHKIIPSAPLVPQNDPTTLFTGSGMQP
                     LVPYLLGAEHPEGKRLANSQKCFRAEDIEEVGDNRHTTFFEMLGNWSLGDYWKEEQLS
                     GFFEFLTKEIKIPKEKLYVSCFEGDTSLNLPKDTESAEIWKKIGIPENHIFFYDAKKN
                     WWSRSGTPDKMPVGEPGGPDSEVFYEFKSVEHDQKFGEKCHPNCDCGRFMEIGNSVFM
                     QYKKTANGFQLLPRRNVDFGGGLERITAASNDSLDIFEIDALKDIKSVLEDLETKSPG
                     RLASKSALYSYRVVADHVRAVVFLISDGVTPSNIDRGYFARRLIRRAVRHLDLLGIGE
                     GNLHRLVTPIARAYREAYPAVQEQALNIENEIKNEEDKFRKTLKAGLKEFEKLSTTPI
                     SGKDAFMLFSTYGFPIDLSLDLAKEKAIVIDIEGFKEEYKKHQDISRQGADKKFKGGL
                     ADTSEMSVKYHTATHVLHKALREVLGTHVEQRGSNITTERLRFDFAHPQKMTDEEKAA
                     VEEIVNEKINAALPVNKVILKKEEAEKSGALHFFGEKYGDEVSVYFIGDSLENAYSKE
                     FCGGPHVTNTSVLGTFKIQKEEAVSAGVRRIKAILK"
     gene            complement(12622..12882)
                     /locus_tag="VI981_02050"
     CDS             complement(12622..12882)
                     /locus_tag="VI981_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479115.1"
                     /translation="MYYTYVLQSKKDGRLYTGSTKDLRKRFKEHEGGRTTYTREHGPF
                     VLIYYEACNNETDARSREKYLKTGMGKRYIKNRLKRFLSLTG"
     gene            complement(13222..14769)
                     /locus_tag="VI981_02055"
     CDS             complement(13222..14769)
                     /locus_tag="VI981_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY4479116.1"
                     /translation="MRIRETSEFFLLFNMGSQTADLIRIRVSECGVFCLMADPAEITA
                     KDVAKLKPKGIILSGGPGSAVYERPPFDEKIFDLGIPVLGICLGFQMFARWLGATVRK
                     AKHGEHGQFKAKRVARADSPLFRGLGDMFDVLQNHGEEVCCGGGLTPLAKTDSCLAAA
                     SWRHLYGLQFHPECWGTTVGRRLFENFLFDICGAHDRYPVRKVADRKIAAMKYRFGLK
                     KKVLLALSGGSDSSVVAYLLMRAIQNRSRLRAVYIKGLDRPQDEADVIRYFGKQKHLE
                     LKVVDGTRELLKELQGVYSMRRKRLVMRKVYDRILNREIRRFGAGFIAQGTLYTDISE
                     SGAGAKTGARKAKIKQHHNIGNKFTVPEIRLLEDMVKDTARNLGRELNVPRWLLKRHP
                     FPGPGLTLRIIGAVTPEKLRMARIADTIYREQLLKYKLYDRIWQAGVVVTDTVVPCFR
                     GDDAAQGRDIQCWAFTSVDGFTARPFFFEENFAVELSRRIMHEVPGAGKFGFSFAEKP
                     LTTIEWG"
     gene            complement(14920..>15177)
                     /locus_tag="VI981_02060"
     CDS             complement(14920..>15177)
                     /locus_tag="VI981_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479117.1"
                     /translation="PLGFWREVRGTVFQTLRHMFAIPLFAGVVIILLIDYALGHSYLR
                     TGLLGLALFVFFCAIVHRTRKEHRSMDNRRAKLPNLSSKSA"
BASE COUNT         3842 a         3673 c         3083 g         4579 t
ORIGIN      
        1 cgcaggcacc gtaatcacag cctcggtcac tttgtcaccg aggcgggcct ctgcgtcggc
       61 tttcagtttc tgaagaatca tcgcggaaat ctgttccgag cgatgccagg catcacccat
      121 ttttacttca atgcctccgt ctgaagacct cctcacttca aaagggacat ttttaatatc
      181 ttgttgaact tgaggctcgt caaaattatg accgatgaag cgtttaatct gataaaccgt
      241 atttttagga tttgtaactg cctgacgctt agcaagcaat ccaacaagtc gctctcctgt
      301 tttcgattga gcaactattg aaggagtcgt gcgcgcgccc tcggcgtttt caagaatctt
      361 cggctagccc gcctccatga ccgcaacggc ggagaatgtt gtaccgagat cgataccgat
      421 tatctttgcc atataatttg tttaatagtt aatttcacaa taataaaaat cttcgacaat
      481 catgccgaaa tagcgtttag cgtaaagcgt ttagcgtata gttagagaaa aatttgtact
      541 ccctaaccgc taaccgctaa accctaaccg cttctgcgag ctggagccct ggttggttgg
      601 ctcactaaaa aattaaactc aaaatatgga gaaactttgg gttcatggta aatatatgga
      661 gcaaaatttg cgacttgagg actgtacggc tctgataagg agagtacctc ttgatttgcc
      721 gtaaaggtat caggagaaat tatttcagtt tttatgattt ttccgcgaat ttgaccgtga
      781 aattcgacaa ccagaaaagt agcccggata aactctccgg tttcagtgcg aaaaatcttt
      841 tctactattc gcttaagggg actttctacc aacatttaat tacagtatat caggctattt
      901 tggtatgtca aaaatttggt tgacaactta taagatatat ggaatgatcg gcatggcgct
      961 gaatcgtatt cggcgaaagc tttcctaaac ctcaatccct ccatctctat gatctacgga
     1021 aacatttctt ttctaaacgt caagaccaag cggcagagaa ttctggcaag aatcttggga
     1081 acaattacct caacgctcat gtccattttc ttcggctgcg gcatgttcat gctcttcaga
     1141 caggagcatg cgtctcccct tgtcgctgga atcgctgggg tagcagtgtt cgcccttacg
     1201 ttctggttct tggtgatcgt cctcgtgtgg aagcagacaa gggcccctaa atagtcgtta
     1261 ccgtccagaa atcttgggcg ttttttattt ctaaaagcta caagctactc gcatcagctt
     1321 tcttgaattc ccccactttc accttcttca ccttcggcgc tcgaagtaac tttccgctaa
     1381 ctttgcttat tttgtaattt tgcaaaatac tagaataacg ttacgttcaa gacaccttgt
     1441 taaacaccga gtgtcacaca tggacattac cctggtgctc tgccccaggg gactatattt
     1501 cattgtattg acctaatcgc atctcgtgat attgtttttt tagatacaca aaccgactac
     1561 ctgccatgaa tatcgaaatc gggacaattg tccgcagcgg aacaaaaaaa gcgcttctgg
     1621 aaatctgcgg cttcacaaca gatggaaaat atctcgccag aaaagtcaga gtcgtgatta
     1681 aggaatcggc tctgctcaaa tacttcaaac tcgagatgct ccagcctggc tccactctgc
     1741 tcctgccaaa tggcctaaaa attcggctga cgttaaaaag cactgctagc acataggcta
     1801 acactgtagc agacgaattc atcatcacga gcgatgaaga gattgaagag gccgaagcgg
     1861 aagaaaaagc cgcaaaagac tacgccgatg tggcgacccg acgagaaagt ccttccagct
     1921 cacgcatcgg tcacgctcca agtcgtcgca cctatcgtcc tccattgtcc aagtaattct
     1981 tgggcttttt attttctaaa agctacaagc tattcgctat cagctttctt gaattccccc
     2041 acttttacct tgggcgcgcg aagctgtttt ccgtttaatt catacccctt ctgaattact
     2101 tccattactt tattattttg ctcctcattt tctacgggca caaattcttg agcttcatcg
     2161 cgcattggat caaattgaag gccaatcgga ttgacatctt tcaatccatg agtttcaaga
     2221 actgttttca actgatttga aatgtattct actcctatgc gccagttttt atcaactttc
     2281 tcccacgctt ccttgttcgc catagccatt tcaaaactct gaagtacggg caaaagttcc
     2341 gtcacaatat cctctttcgc aaatttcaaa aattcggctt gcgcttccct atcccgcttg
     2401 cgcgcattta aaaattctgc tttgtctctt tgccagccgt cgagatattt ttgcttttct
     2461 tcaagggctg tttttagctt ttccttgagt ttcgtgacct ggcttttgag atcattatcc
     2521 tcctcaatca agccgtcagg atcaaggtcg gacctttttt ccgatttaag gtccgacctt
     2581 ttgactttga gaggattccc ctcttcatca gtagggacaa catcagcagg atcgttgttt
     2641 acatcatcat tcatgaatgt attttatact gttctaatag caaaaaccaa ggttgaacct
     2701 ttttgccgat ccaaggttca accttggttt tctgacttac aaacttaaac tacctctttg
     2761 accattgagg ggctttcctt gccttcttga gaccgaattt gcgtctctct tttgctcgag
     2821 gatcacgctt taaaaagcca gcttttttca tttgctttcg gagttctccg ttgtgcacga
     2881 cgagcgccct tgaaataccg tgtcgaagcg cttctgcctg tgaattaatg cccccacctt
     2941 tcagcagggc agtaatttta aatttttccg ttatctttaa tttagaaatg ggttcgagca
     3001 ctaaactgcg cagatgcact gtcgggaaat aagcctcaag tcctttctcg ttaatcagaa
     3061 gcgattgctt aggcgaaacg gtgatgcgaa cacgggcagt cgaagtcttt cgtctcccga
     3121 tggtttctat ataaattgtc ttttcttttg tgggttgcat gattattcag tgacgatcaa
     3181 acgttttaac atatctggtc tcaatttatt gccgggaagc attcctctta ctgcaatttt
     3241 aaaaagttcg ctgtatcctt ttttttgtgc gatttgttcc atcgaaagaa agcggagccc
     3301 tcccggatat cctgaatagc gggcatattt ggtgtcacgc attttctttc ggggaagaac
     3361 gattttcgaa caatttgaaa tagtaactac tatatccttg gggtttgcgt tgcgcacaaa
     3421 atttgtttta ttttttccca taagtaacga cgccgcttca cttgcgactc gtcccaatct
     3481 tttgttttgt gcatcaattg tgtatttcat cattctaata tactaattca taccaattat
     3541 acgaatggag acaaatgcat ttttctttat tagtttcatt ggtatacatt cgtatattgg
     3601 tattatacaa actcaatttg cgctaatttt gcaccgtccg acgcccgtcg ccccattttc
     3661 gtgattctcg tgtagcctcc ttgccgagtc ttatgcttcg ttgaaatatc gctaatcact
     3721 ttcgaaacaa gccgcacgct accaagacgc gctataagca atcgccgtga cgagagggta
     3781 tcatttttgc ctttagttat caatttttca ataaacggcc gaagttcttt agccttggct
     3841 tcggttgtct cgattttgcc atgcaaaata agcgacgagg caagcgaacg aagcagagcc
     3901 ttccgcagtc ccgcttctct tcctaacact ctgtttttct tgtgatgtct catttgcttc
     3961 gcaaaaatac taatttacta attgttacaa atatacgaat ggatacgaat aaatcacatt
     4021 agtattattt gtatattagt attcattcgt atattggtat ttactttaac gtaatcccat
     4081 atcccgccaa tactttcttg atctcctgca ctccctttga accgagcccg tcgatgtcca
     4141 agatatcctc ttctttcttt ctcgccaagc cacccaatgt tctgatgtta gcattggtaa
     4201 gtgcattaag cgttcttgga gaaaagccga tcgtttcgat tcttgttttc aaaaattctg
     4261 gatcaatttc ttttacggcc gcaatcggag attccgcctt tgctagaggc gttggttcaa
     4321 ccagttttac ttcaggttcc tcctccttga agccaacaat ggccttgagt tggttgatca
     4381 ttatttcgat ggatttctcg agtgcttctt ttggagtgag cgttccgtca gtttcgataa
     4441 atatctttac gcgattgaaa tctgttcggt tgccaacgcg catattttct acctcatagt
     4501 tgacgcgtct aatcggcgtg aaaacagaat cgagcgtgat tgagccgata tcaactcgat
     4561 ctttttgaag ctgttccttt gcgacgtagc cgagtccttt ttccacggtc atctccattg
     4621 aaagttcagc acccttgtcg gtaagtgtcg cgatgcgaag ttctgggttc aaaatctcaa
     4681 cctgacccgg cgccgttata tcggctgctg tcacgtcttt tactcctttg attttgagcg
     4741 tcagaatttg atcttcgtct gtgagcattt tgatacggag acgcttgaga ttcaaaagaa
     4801 tagttataac gtcttctttg acgccaggca gtgtcgagaa ttcgtgatta acgccctcta
     4861 ttttcacgcg cgtcacggca gcccccggga gggacgatag aatgattctt cgaagtgaat
     4921 ttccgagtgt gtggccatat cccggatata acccgtcaat ttcatatact ccactcattt
     4981 tctcttcgtg gatgatttta ggctttgagg gcaatactac gttgtgctct gacatagatg
     5041 tgcgtataaa ttaataataa ttatcgttta taaaattcaa caattgcatt gatatcgaaa
     5101 aaagtctccg atccctcacg tttgggcggt gttgcgatct ctgccacctt ggtttccgga
     5161 ttaaatttta tccagttcgg cgtacgaacc tctttcaatt tttcgtcaag attctggaaa
     5221 agcgctttgt tcttgcttgc ttcgcggatg gcgaccttat ctttcggaga caccttaaac
     5281 gacgcaatag aaactttttt gccgtttaca gtgatgtgtc cgtggctcac catctgccga
     5341 gcagcggcgc gcgatacgac aatacccata cgatagacga cattatcgag tctgctctcg
     5401 agagttctat agagttcgtc agcagctttc tgctttttct gggcaatcgc gtttttgacg
     5461 taccgtgaaa actgccgttc acctaccccg taggtgaagc gtgctttttg tttttccaaa
     5521 agctgaatgc cgaaatcact tttcgggcgt gaaattattc ttgtctttcg tgccgctcgg
     5581 gcctgaaatt tggcggattg agttttgtcg aaaactcctg ctcccaaccg tcgtgctatt
     5641 ttgtatcgtg gacctatttt catgccaatt tattatgaac gaagtgaata aataaattga
     5701 gcacccagca ccaattttac cgaccgcctg cgtaaagcca tttcgcaaaa tactctatta
     5761 aattttttca ttatcgtttt catagtttct gtgaaattgg tgcatgggta aagtaaatca
     5821 tattcgcttc gctcattgat tttctaaaca cggcggggct ttttcggccg cggaccgtta
     5881 aacggcaccg gagttgcatc tctaatagaa agtagattta ttcctttcga aagaaaacct
     5941 cgaacagacg actccctacc cgaaccaact ccttttatga cgacgtcaat ttctttgagc
     6001 cccattgctt gggctttggt tccgagcgtc tccccaacct tcgcagccgc aaaaggcgtt
     6061 cctttctttg cacccttaaa tccgagtgca ccacttgatg cggcgcccac gactcctcct
     6121 tgcttatcgg taagcgtcaa aattgtattg ttgtatgttg attgaacgta taaaactccg
     6181 cgatccagtt ttttcttcgc agatttaccg ccgccagatt ttccggcttc gccctctccc
     6241 gtctctcctt ttgttatgat tcgcttctta cccatgattg attttcttac gaacgaagtg
     6301 agtttgaaaa tcgtcacccc gttaaataaa accttcggtt ttgtccgcct taggcggact
     6361 atttaacggg gtaataaaaa tctaattcgc tttgctcata atatttttat tatttcttat
     6421 ccacagcctt ctttcctgaa cccattgtct ttcgtacatt accgcgaact gttcgagaat
     6481 tggtcttcgt tcgctgtgca ttgactggca attttcgcaa gtgcctgacc ccgcggtagc
     6541 acttcacatc tttaagacgt ttgatgttac ctcctatctc gcgtttcaaa tccccttcaa
     6601 ctttataggt ctcgacgatc tttcgaatct cattttcttc aactggcgaa atttcttttg
     6661 gtcgctttcc aaattcaacc ttggcctcgg tcaatatctt ttgcgcacgc gttctgccaa
     6721 tgccgaaaat agcggtcaac gctatttcca ttcttttatt ttccggcaat gttattcctg
     6781 aaatacgcat gattgatttt cttatccttc gatatgactc aggataaccc tgagtcccat
     6841 tgaagggtta cgaacgaagt gagtttgaac ccagctccac ttttgaatca tcgagatttc
     6901 cattgatcaa aagtggagct gggcgctttg ctcataacat ttttattatc cctgcctctg
     6961 tttatgacgc ggattgcttt tgcacgtgac atagagatgc cccttacggc gcactactct
     7021 acattgggca caaatttttt ttactgacgc tttcacatgc atgaaaaaag atattacaca
     7081 atacttctgg ctttgtctat aaacacaaag aaccctcgcc gggcctctgc taaagccttt
     7141 ttataattct gcctttacct ccatacggat cgagctccac caagaccttg tcgcctatca
     7201 gaactcgaat gcgattcatc cgcattttgc ccgccagata cgcgagcatg acctcgtcct
     7261 gaccctcaag ttttacccga aaaaatgtgt ttggaagggc ttcagtcacc accccagaca
     7321 cagttgcgac ctctttcttt atcattaatt taaaagcagt tttgaaatag taacaaggac
     7381 ttctccagga gtcaaccttg aagtggatat catcctgctt aagcgaagct tagactcaag
     7441 gtcttagaat tcggcccgtc ccgcaaggtg ggcaagtaag cctagagacc acttcagggt
     7501 aaaccccacc gaatacccta ctttttagct attatttcga tcttatcctt gtcgcttgga
     7561 aaatgcattt tccagaacgg tcggagtatc acgtccgcac cttgaactgc cggaaatgca
     7621 tcgagaatgc cttcgagaga ttttttgggc ttccccgcaa gtgcgtcttt cagaagggcc
     7681 tcgtcgaaat cccagacaag tgcggctccg ccgacaacag cgacccgcaa ctcattggca
     7741 gcccatggtt taacctgcgg atcatcaata attctaacat caagagtttc gggattttca
     7801 aaatgtaatt ttgcgtcctt ataatctttg atgtactttt tggcaagcgc cgccacaaat
     7861 gagtctttgc gaattataat tccgtaaatc ctgccttttt cctttacctc gacttgattc
     7921 cccgaaacgc tatcttctat gcccttttca aactcatagt agacggcctt atcgtataaa
     7981 acatagtcac tcggaagatt cgatcgcact tcgcgaagca tttcggtttt aagcgaccct
     8041 tcaatttctt tttgagtatt agatcgtgtt tggtcgtcga taatacgcgt ttttccttta
     8101 agtccgccgg acattgtcgt ctttgagcga gcataaatgg tttcatattt ttgaccgtct
     8161 cctttgaaac ccggtattgt ccagtccaca agtccgatat tgtattcttt cccagcctga
     8221 tctgcatata cagtcacctc tatactgccg ggagtcgctg aaccattggt tcttttaagt
     8281 cctgggataa cgatcgattc gctaatgcgg aaaatcaacc ccgttgggct cataaagcga
     8341 gtatttttta tgagacgttg agactctgtc gaaaaattat tgtatacaac aattttccca
     8401 gacgcttttt tctctatatc tttctcctct gtaccctcga cttttttgct tccgtctttt
     8461 tcaagcatga cgatctcatg aaaaagatca gctgtggcac cttttttcga ttgaaatgtc
     8521 gaatcaacgt taataatttg ccgcttcgcc tccacggaaa ctgtcgctcc agtgaaaaac
     8581 gccgagaacg ttaagaaaat aatgaccaaa accacaaaga agccgataaa aagcagtacg
     8641 cgtgaacggc gtttaggtga cctctttttt ggctccaatt cgactagagt agagggttct
     8701 tctcttctct ctggtctttc cacgcgaaca cgttttccgg ctaaatggat atttctgatc
     8761 gaccgccttt ccggcggcaa tacgtcttct aaaatgtttt ttgccatact taaatgtaag
     8821 tttatcagaa ctttacttat ccggccacag agaaaactaa ctatatatgt ggtttacaaa
     8881 aatagaatcg agggccagga taacatcctc tttgacattc ggagcttgtg ataaattttt
     8941 tgcgatgacg gcatggtcga gaagcacaac ctcaaattct tgtcttaaag aatcagcctg
     9001 aacgaacccc gcacggccga taacatcttt gaagtaaagc gctatgtcat catcggcgac
     9061 taaaaatacc ttcgggggaa taaacgcttc tccggaaact ttggtaagca cttcttcgaa
     9121 gagggagacc catttcattt cggcaatatc gagtgacttt tttaactttt cgctaaatgc
     9181 tttatccatt tttccctctg cacgagcttt tataaaagac ctcgtaacct gcggaatagt
     9241 tttcagcgct ttagcggatt gtaaaagaaa ggcattttta ccgataggaa tggaaagtgt
     9301 ttcgacaagg agcccccgct ttacgagcat gatatcagtg acttcacctc caacttcaac
     9361 gaccaagtat tggtcgttta atccctcgag attgcgcatg gttgagaacg ttacgagcgg
     9421 catggtgtga cactcaaacg ctttgaaggt gaaatgtttg tgaatggtcg attcaatgct
     9481 ctccatgaga gattttggag acgcagaaag atacagcata acttcaaccg aagaagcccg
     9541 ctgaccatag ggctttgaaa tctcgtatcc attcaacttt atggtcacta tttttgcctc
     9601 aagtacagtt tcatcggaag tatccgccgt ttttatcttg tcaagaaaga gccttttctc
     9661 gtcttcgaca agtcggccaa caaaaatttc atcaaccgta aatggcaacg gcttcacgga
     9721 tgttacgatt ttagttgtcg acaaaaacca cggtgatgaa aggacgcaga aaacttttct
     9781 gatttttatg cttttcgaac tgacacgctc gagtacgtgg ctttctttaa caagagcagt
     9841 tgcacaggtg ttgagcgcct ttaccatttc atgaatgaat gaagcgcgag aactttccgc
     9901 aataggagcc atgatttcgg tatgcgcacg gtggagcatg ggagtgccac cttgcgtaaa
     9961 gccaacaata gcgccccgca cgtcagcact atttatatga aaaaccattc cgatttcttc
    10021 gtgcgctttt ttagagaaga acgacatgaa aatagtataa atcaaaaggc aaaaatcaaa
    10081 aggcaaaagt ttgatacaaa aaaaagcgct tgcggggcgc tgtacttaaa gttcttggag
    10141 agaaactgct ttgctatcac gaagcaccct tagcacttgg tctctttcat atcctcgtga
    10201 caagctctgt tctgcctgtt cgaggacttg aagcgcgaga gcttcaacgt gctcatcgag
    10261 cgatcccata aattttttat caataaaacg ggagtgtact tcatatgcca cgattcgaac
    10321 ccggagctca agctccgtat ggccatggtt ctttattgag aagtctccgc caagtgcact
    10381 acggctcttg accagtgctt gaagttttac cgtcgcacgc tgcagtccga tgttagccag
    10441 attattcata gcattgctta ataaattcct tgttcattta aacaataaag cgactttgag
    10501 tcaaccccgt tagaagctca tacggggcat taaacccgtg ttcgctaaaa tcaaattaca
    10561 ttcatcccgt tagaagtagc tcgcgattca cgatcacggc ttctaacggt gtcaacaaaa
    10621 aatgaccttc tcctgctcct ctacggcctt gcgtccgggg tttccttccg aattcaaggt
    10681 ccaaccttga tttcaaggtt ggaccttgaa tggttcattg gttcattgag taactacaac
    10741 tataaaatgc tatagcattt tatagttgta gttcagattt tagagtttaa gtagaaaatt
    10801 atgtaaatta tttgagaatg gctttgatac gtcgcacgcc tgcagaaaca gcttcctctt
    10861 tttgaatttt aaaggtgccg agcacgcttg tgttcgtgac atgtggtccg ccgcaaaatt
    10921 ctttcgaata agcgttttcg agcgaatcgc cgataaaata aacactgact tcgtctccgt
    10981 atttttcccc aaaaaaatgc aatgcgccgg atttttccgc ctcctccttt ttcaagataa
    11041 ccttattcac aggaagcgcc gcgttgattt tttcgtttac aatttcctca actgcggctt
    11101 tttcttcgtc ggtcattttt tgaggatgag caaagtcaaa acgaagccgc tccgtagtaa
    11161 tatttgatcc acgctgttca acatgagttc ccagcacttc gcgtaatgcc ttgtgaagca
    11221 cgtgcgttgc agtgtggtac ttcaccgaca tttcactcgt atcagcgagt cctcccttga
    11281 attttttatc ggctccttga cgagaaatgt cttgatgctt tttgtattcc tctttaaaac
    11341 cttctatgtc aattacgata gctttctctt ttgcaagatc aagcgacaaa tcaatcggaa
    11401 aaccgtatgt tgaaaaaagc ataaacgcat ctttaccgga aatgggcgtc gtgcttaatt
    11461 tctcaaattc tttcaggcca gcttttaatg tttttctaaa tttgtcctcc tcgttcttta
    11521 tttcgttttc gatatttaga gcttgctctt gcacagccgg atatgcctct ctatacgcgc
    11581 gagcgatcgg ggtcacgagc ctatgaagat tcccctctcc aatccccaaa agatccaagt
    11641 gccttactgc acgtctaatc agtcttcgtg caaaataacc tcggtctata tttgatggcg
    11701 taaccccgtc cgatataaga aatacaactg ctcggacatg atctgccacg actctatacg
    11761 aataaagcgc tgacttggag gctaagcgac ctggagactt agtctccagg tcttcgagga
    11821 cactctttat atctttaagc gcatctatct caaatatatc caaactgtcg tttgatgccg
    11881 ctgtgatacg ctcaagccct ccaccaaaat ctacgtttct acgcggcaat aattgaaatc
    11941 cattcgccgt cttcttgtac tgcatgaaca cggaattacc aatttccatg aacctcccgc
    12001 agtcgcagtt cggatgacat ttttctccaa atttctggtc atgctcgaca cttttaaatt
    12061 cgtaaaaaac ctcgctgtcg ggtccacccg gctctcctac cggcattttg tccggcgttc
    12121 cagatcgact ccaccaattt tttttggcat cataaaaaaa gatgtgattt tctggaattc
    12181 cgattttttt ccaaatttct gctgattctg tatcttttgg caaattgagc gaggtatcgc
    12241 cttcaaaaca gctgacatat aatttttcct tgggaatttt tatttctttt gtcaaaaact
    12301 caaaaaaacc tgacagttgt tcttctttcc agtagtcgcc aagcgaccaa tttccaagca
    12361 tttcaaaaaa agtcgtatga cggttgtccc ccacttcttc aatatcttcg gcgcgaaaac
    12421 atttctgact atttgcaagt cgttttcctt cgggatgttc tgcccctaac aaatacggaa
    12481 ctagaggctg cattccagaa ccagtaaaaa gcgtcgttgg atcattttga ggcacaagtg
    12541 gtgccgaagg aatgatttta tgctccttac ttttccagaa ttctaggtat tgtgttctga
    12601 cttcggcgag tttcatcgga tttatcccgt tagagatagg aagcgcttta atctattttt
    12661 aatatatctc ttgcccatgc ctgtttttaa atatttttct cgagacctag catctgtctc
    12721 attattgcag gcttcataat agattaacac aaagggaccg tgctctcttg tatacgtcgt
    12781 tctgccgcct tcatgttctt taaagcgctt ccgtaagtcc tttgtcgatc cggtatatag
    12841 acggccgtcc tttttacttt gcaatacgta agtgtaatac ataacgatga aatctctaac
    12901 gggatgaata tagcacaata ataaaaatac cccagtacta aattttatcg ccagaaaagt
    12961 ggcgacgcgc tccccgaagt gagccgcttc gcgtctacta cggggcaagg tcgccttaag
    13021 ttctacttaa cagcctacgt gtcccgtagt gagccttgct ctattactgg gattcggcca
    13081 cgcgcgtcgc gagaagatat ttcctgctgt aaaatgcccg aaaatgtatt tcaagtatag
    13141 caaaatttag tacggggtta ttgttttgcg ttcatcatgg cgcgggcaaa acaataaaaa
    13201 accagagtat aaaactctgg cttagcccca ctcgatagtg gtcaatggtt tttcggcgaa
    13261 ggagaagcca aatttgccgg ctcccggaac ttcgtgcatg atccttctcg acagttctac
    13321 ggcaaagttt tcctcaaaga agaaaggtcg ggccgtaaac ccatcgacac ttgtgaaagc
    13381 ccaacactgg atatctcgcc cttgtgcagc atcatcgccc cggaagcatg gcacaacagt
    13441 gtcagtgaca accacacctg cctgccagat ccgatcgtac aacttgtact tgagaagttg
    13501 ctcgcggtat atcgtgtcgg caatgcgggc catccgaagc ttttcaggag tcaccgcacc
    13561 aatgatacgg agcgtcaggc ccggcccggg gaacggatgg cgcttaagaa gccaccgggg
    13621 aacgttaagt tcccgcccaa gatttcgcgc cgtgtctttg accatatctt cgagcagtcg
    13681 aatttccggc accgtaaact tgttgccgat attgtggtgc tgtttgatct tggcttttcg
    13741 tgcaccagtt ttggcaccgg ctccgctctc ggaaatatcc gtgtagagcg tcccttgagc
    13801 aatgaagcct gcgccgaaac gccgaatctc gcgattcaga atccgatcgt aaactttacg
    13861 catgacgagc cgtttccttc gcatgctgta gacgccctga agctccttca agagctcccg
    13921 agttccgtca acaactttaa gctcgaggtg cttttgcttg ccgaagtaac gaatcacatc
    13981 ggcttcatcc tggggccgat caagcccctt gatgtagacg gcacgaagtc gtgagcggtt
    14041 ctgaatcgcg cgcatcagga gataggccac aacagatgaa tcagaaccac cggacagcgc
    14101 gaggagcact tttttcttga gtccgaacct atacttcatc gcggcgatct ttcgatccgc
    14161 tacttttcga accggatagc gatcgtgcgc accgcagatg tcaaacaaga agttctcgaa
    14221 gagccgacgg ccgacagtcg ttccccagca ttctggatga aactgcaaac catagagatg
    14281 ccgccaagac gctgcagcga gacaagagtc tgttttggca agtggtgtga gacccccgcc
    14341 gcagcagact tcttcgccat gattttggag aacgtcgaac atatcgccca acccgcgaaa
    14401 aagcggagaa tccgctcgag caacacgttt tgctttgaac tgaccgtgtt caccatgctt
    14461 ggcttttcga acggtggcac caagccatcg ggcaaacatt tgaaaaccca aacatatgcc
    14521 gagaacggga atgccgaggt caaaaatttt ctcgtcaaac ggcggccgct catagactgc
    14581 cgaacccgga cccccggaca agatgatccc cttcggtttt aattttgcca cgtccttggc
    14641 cgtgatctct gcaggatcgg ccatgaggca aaaaacaccg cattcgctca cgcgaattct
    14701 gatgaggtcg gccgtttggc ttcccatatt gaagagaaga aagaattccg atgtctcacg
    14761 aattctcatt tcctcctgta gcccggagag acttttttca gttgtatctt ttagaacgcg
    14821 aagcatttgt gcctccttcc taattggttg aagttgttct tgttgctaca tcaaactcta
    14881 tcgtttccta tctctttacg caagcaaaaa agccgacgac taggccgact tggaggataa
    14941 attggggagc ttggctcttc tgttatccat agagcgatgc tctttccttg tgcgatgaac
    15001 aatggcgcag aaaaaaacga aaagcgcaag accgagaagc ccagtacgca agtaagaatg
    15061 gccgagcgca tagtcgatta acagaatgat caccacaccc gcaaagagcg ggatcgcaaa
    15121 catgtgccgt agagtttgaa agacggtccc gcgaacttca cgccagaatc cgagagg
//
LOCUS       DATDSU010000059         9949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2390514, whole genome shotgun sequence.
ACCESSION   DATDSU010000059 DATDSU010000000
VERSION     DATDSU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390514"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="murB"
                     /locus_tag="VI981_02065"
     CDS             complement(<1..785)
                     /gene="murB"
                     /locus_tag="VI981_02065"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY4479118.1"
                     /translation="MKLEKNVPLAPYTTMRIGGNADVFFHVETLADLAFASKYIREKK
                     LAFFVLGGGSNVLIRDEGFHGAVIHPRLKGIKVKQLGSFRFKVEAAAGERWDDLVAYT
                     VGQGFYGLENLSLIPGSVGAAPVQNIGAYGVELKETLESVEIFDMNDGKIKVLSNEEC
                     KFGYRDSIFKKPKGKYYIITKITLELASKGNIYIGYRDLMLRFKDVYSSKLTPKDIRE
                     AVIDIRQKKLPDPTKIGTAGSFFKNPIVTKLQFQKLKMRFPALP"
     gene            complement(1148..2179)
                     /locus_tag="VI981_02070"
     CDS             complement(1148..2179)
                     /locus_tag="VI981_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479119.1"
                     /translation="MKRLLLFVAKDMGSSNVTLDLALKCVAEGHEVHAVTEGLASKRY
                     ILGGITPRFVGTVNFKTEPFTLDVPKMLEALKPDVVVTGLGSPINLEGAFGVVSNALG
                     MPLVECEDFWNGHSRSAANPDLLLTLDEYAAELMRKSHPRTTVEIVGNPGVAGYELAP
                     EVVGLRRHFDRVFVYPGGGDYTTDEIRFLAACLKQTSHNWCLIPRFHQKWMKEKNPAD
                     GRPYGDMWKEMLQEFRERVVYLDSVKVTETIVAAADGTFSGFSTLLTTSAFHNCPTGS
                     LHLPSVMASLKAQANLNEVPIVKLGCAHSLAEPVDLAGLAPPPPENVAKLKPYNVDAA
                     YAALMAVFK"
     gene            complement(2334..4496)
                     /locus_tag="VI981_02075"
     CDS             complement(2334..4496)
                     /locus_tag="VI981_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY4479120.1"
                     /translation="MRLNKYLKYIVTAGLFLVPFIPLIISRDMLFPFITGKNFWFRII
                     VEIIFAAWLLLMVRDRSYRPIFSYLAGALGLFLVVITIADIFGANPYKSFWSNYERME
                     GLITHLHLFAYFIVLGSYVKTEKFFERLLQTSLGVSVIMGFYGILQLTGAVKINQGGV
                     RLDATFGNATYLAIYVFFHIFIALFLMARWRGHKAVSYIYGLVVVLHLFILYHTQTRG
                     AMLGLAAGLLVAALLYIFLSREKNTLRKISIGIIVALVVFGGAVLLLRNSNIIKKFPP
                     LERLSSISLKEGTARFRVWNVAWHGVKENPILGWGQEGFNFVFNKYYDPQMYTQEPWF
                     DRTHNIAFDWLVAAGILGFLSYLSILVLFFVVLWKKTQMTLLEKAILSGAIVGYTVHN
                     IFVFDNLGSYIIFFTLLAYVHYRATIDKQVTVSEEPHAASFRLALPVVVLFLTVAIWF
                     GNAKGMLTAKTLIDAMQSHPEGATKNIELFKKALSYNSFGSTETREQLAQMAQSALGA
                     DVDNSVKEGFFNLAFLELKKQVEATPEDARYQLFTGVFLSRFGQGPLALPYLEKALEL
                     SPKKQSIYYEIGILQINAGKMAEALETFRKAYELAPTSPESRQYYALAAVYAKRSDIA
                     KEVLGGKDYTLDDIFIAAYVKLKDFKKVLEIYTDRLAISPTRENYVGISVAQLKLGNK
                     TKAIEALQKAAEVDPEFKKQADFYIKEIKEGRDPTGSQ"
     gene            4893..5552
                     /locus_tag="VI981_02080"
     CDS             4893..5552
                     /locus_tag="VI981_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY4479121.1"
                     /translation="MLRGRDYRVATVAGLLTAVFVSGMLFFSWREALTYSPILFVTAP
                     LIYVAGIRLGFFLGKKYKFFEQFGRFAVVGFFCASIDFFILNALSELSGIERGFLVGW
                     VNVPGFSVALVVSYLLNHRWVFGNNNLLDQNESGLKKVPNLLSAFRHFPTFLVISVIG
                     LFLNSGIIIILTTYIPSQNYNDELVLNVAKVVASAVVMISNFIGYKFIAFRKKMERNP
                     K"
     gene            5549..6253
                     /locus_tag="VI981_02085"
     CDS             5549..6253
                     /locus_tag="VI981_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4479122.1"
                     /translation="MKKLSIIIPVYNEKITIEELLKKVEWAPTPGLEKEIIIVDDCST
                     DGTREILEKIGERIRYKVFYHAKNQGKGRALRTGFASATGDFIIIQDADLEYNPEEYV
                     NLLGPLLDGKADVVYGSRFISALPHRVLFFWHFVGNKVITNFSNMMTNLNLTDMETGY
                     KAFNEEALNLIKHKLSSNRFGIEVELTARVAQAHLRIFEVGISYSGRTYGEGKKIGWR
                     DGVAALWFIIKYNLFT"
     gene            6254..7819
                     /locus_tag="VI981_02090"
     CDS             6254..7819
                     /locus_tag="VI981_02090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY4479123.1"
                     /translation="MDKLTLTLGALLIVYNAYLAGNSIISRLRIAVATRQGRIGYAIT
                     IGLGLIVFIGFILAIFSILYAWLLWLLLLISLIIYRKKIYSHILFFLRLRLGTGLSMV
                     KAALRPYWFLKTLIVIWLLGNFLLAFVPLTGHDTLDYHLPIIESVANSHGLDFARSVE
                     DFNYLPILGEILYAIPTVLFSEHAAPYVFQILQYLVIVFVSLIVWNTVLPRVKNKLFG
                     FASVLTVLSLMDFQREVLHGGYIDVLGFLFGLAALFLLIDHLERDAGSKRGYILCGLF
                     LGLALSVKYTGLFFVAISLLFILIHHLKRKETFLGLIRDGLYLGIPAVIVSGFWYVRN
                     LFSFGNPVFPMWSDAETTASVGLFIMDRTVGNFLYFPFYRYAQWFLQEVETSSRLIVL
                     GLFIVLYLLIIILAIAKRRFTLTSVFLFIFVELYLLFLFISSHQYRFLIPATIGASVL
                     VALLADSFASWIENKINTDSRKKIFQGVISGLVLAGFLLVYLGNLHYFEVKWKYVLGK
                     TTTERYIHDIGGQ"
     gene            complement(8487..8888)
                     /locus_tag="VI981_02095"
     CDS             complement(8487..8888)
                     /locus_tag="VI981_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479124.1"
                     /translation="MSKRESIHIENSHIQGVLKQESVEGKRLLEPLKEFAKINGVPFN
                     ILEDKNVDNDAEVHMHEADLWHCLEGEVTFICGGQLVNGKAKVNKDGSLDEREWKAKE
                     IKGGREMILSPGDWLFIPAGEPHKHSAKGVA"
     gene            complement(8893..9837)
                     /locus_tag="VI981_02100"
     CDS             complement(8893..9837)
                     /locus_tag="VI981_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4479125.1"
                     /translation="MTLKILNTIGEIYTPEAKTILNQMGEVTYATPLQDELEKIVADY
                     DVTVIGLGLNFDKEVLSNARNLKVIATATTGLDHIDVVHAESKGIKVLSLKGENAFLD
                     TITGTAELALGLMIDLVRYTPWAFESVKQYEWKREDWRGVSLYGKTLGVVGLGRLGKM
                     TARYGKAFGMAVIYSDPNVDSNEYRKVDFQTLLAKSDFISIHVHLAPSTENLFNAAAI
                     AKMKKTAYLINTSRGKIVNEADILDALKSKQIAGYATDVLSDELDFETEGFKNHPLVE
                     YAKKNKNLIIVPHIGGMTSDSRSATDVFIAEKLKTILK"
BASE COUNT         2682 a         2310 c         2076 g         2881 t
ORIGIN      
        1 ggcaatgcag gaaaacgcat ctttagtttt tggaattgca gttttgtcac aatcggattt
       61 ttaaaaaaag aacccgccgt gccaattttt gtcggatccg gaagtttttt ttgtcgtata
      121 tcgatcaccg cttcccgaat atctttgggc gtgagtttcg atgagtagac atctttaaat
      181 cgaagcatga gatccctata gccgatataa atatttcctt ttgaagcgag ctcgagcgta
      241 atcttggtaa taatgtagta cttaccttta ggttttttaa aaatactgtc gcgatatccg
      301 aatttgcact cttcattcga cagtactttt attttcccgt cgttcatatc aaaaatctct
      361 acgctctcca gtgtctcttt gagttccact ccatacgcac caatattttg aacaggagcc
      421 gccccaacag atcccggaat aagcgagaga ttttcgagtc catagaaacc ctgtccaacc
      481 gtgtaggcaa cgaggtcgtc ccagcgctct ccggccgccg cttcaacttt gaatctaaaa
      541 cttccgagct gttttacttt tattccctta agtctcggat gaatgactgc cccatgaaaa
      601 ccctcgtcgc ggataagcac atttgaaccc cctcctaaga caaaaaaggc aagctttttc
      661 tctctaatat atttcgaagc aaaagcaaga tcagcaagcg tttcaacatg aaaaaacacg
      721 tcggcattgc cgccgattcg catggttgta tagggagcca atggaacatt tttctcgagt
      781 ttcatggggt agtgaaaaat ggcatcgctc cacagggctc aattaccact tctgttatat
      841 tagtcgtaaa attcacgttt atcagtaact ttaactttga cccgacctct aatgccattt
      901 tctactaccc cattcatccc gttatgtata gcatggcgtg taaaaaagaa aagcgctgtt
      961 cgacctttat acttacactc tgtaaaacaa caacaaacat acatacatta attttacgcg
     1021 gccgcgtaaa attaatgtat gttggaattc atgactcttc gcggattcct ttaagcttac
     1081 gaaaagaaaa tcgccgagca ccagtttggc gcagggcgag tgttggaatc tagatcccag
     1141 tcccagatta tttgaaaact gccatcaggg ccgcgtaggc cgcgtcgaca ttgtagggct
     1201 tcagtttcgc cacgttctct ggcggcggcg gcgcaagtcc tgcgagatcc accggctcgg
     1261 cgagactatg agcgcacccg agcttgacga tcggcacctc attgagatta gcttgagcct
     1321 tgagggatgc catcacactc ggcaaatgga gagagccagt tgggcagttg tgaaatgcag
     1381 acgtagtgag aagtgtgctg aaacctgaga aggttccatc agcggccgcc acgatcgtct
     1441 ctgtgacctt gaccgaatcg agatacacaa cgcgctctct gaactcctgg agcatttcct
     1501 tccacatgtc cccgtacggc cgcccatcgg caggattctt ctccttcatc cacttctgat
     1561 gaaagcgcgg aataaggcac caattgtgtg aggtctgctt caagcatgcc gccaaaaacc
     1621 tgatctcgtc agttgtgtaa tctccgccac ccggatagac gaagactcga tcgaaatggc
     1681 gacgaagacc caccacctcc ggagccaact cataaccagc cactccaggg tttccgacga
     1741 tctcgactgt tgtccgcggg tggctctttc tcataagctc tgccgcgtat tcgtcaagcg
     1801 tcagcagaag gtcaggattt gccgcgctcc gcgaatgacc gttccagaag tcctcacact
     1861 caacgagcgg cataccaagt gcatttgaca cgacaccgaa tgcgccttca agattgatgg
     1921 ggctcccgag accagtgacg accacgtcag gtttcaatgc ctccagcatc ttcggaacat
     1981 ccagcgtgaa tggttcggtc ttgaaattga ccgtgcccac gaatcgcggg gtaattccgc
     2041 caaggatgta tcgcttcgag gcgaggcctt cggtgacagc gtgaacttcg tgcccctccg
     2101 ccacgcattt caaggcaaga tcgagcgtga cattggagct acccatgtct tttgcgacga
     2161 acaacaggag tcttttcatt cagtatgctt tccattaagg agtttctgtc gaaagggtcc
     2221 aaattgtctt cggcccttag atttcaacgt aaaaccggca cacgccggct tgtaaatcta
     2281 ctccgcagag tagcataata tgtaccatta gtcaatatct ctcgcgaaaa tttctattgg
     2341 ctacctgtcg gatctcttcc ctctttgatc tccttgatat agaaatctgc ttgtttctta
     2401 aattccggat caacttcagc agctttttga agcgcttcta tcgctttggt tttatttcca
     2461 agtttaagct gtgccactga aataccgaca taattttcgc gtgttggact tatcgctaag
     2521 cgatcggtat atatttcaag aacttttttg aaatctttca gctttacata tgcagcgata
     2581 aaaatatcgt caagcgtgta atctttgcct ccaagaactt ccttggcaat gtcggagcgc
     2641 ttggcgtaga ctgcggcgag cgcatagtac tgccgagatt ccggagaagt aggcgcaagt
     2701 tcatatgctt ttctaaaagt ttcgagcgcc tctgccattt ttccagcgtt gatttgcaaa
     2761 attcctattt cataataaat ggattgtttc ttgggcgaga gctcgagtgc tttttcaaga
     2821 tatggaagag caagcggccc ttgtccaaat cttgagagaa agactccggt gaaaagctga
     2881 tagcgcgcgt cctcgggagt ggcttcgact tgttttttaa gttccaaaaa agcaaggtta
     2941 aagaagccct ctttcacaga attatcgaca tcggcaccga gcgctgactg tgccatttga
     3001 gctaactgtt ctcttgtctc tgtggaacca aatgagttat acgagagcgc ttttttgaaa
     3061 agttcaatat tttttgtggc tccttcggga tgcgactgca ttgcgtcaat aagtgtcttt
     3121 gcggtaagca ttccctttgc atttccaaac cagatggcga cggtcaaaaa tagaacgacc
     3181 acgggcagtg cgagacgaaa cgatgcggca tgcggttctt ccgatacagt cacctgtttg
     3241 tcaatcgttg ctcgatagtg cacatatgca agcagtgtaa agaaaattat gtaactgccg
     3301 agattatcaa agacgaaaat gttatgaact gtgtatccga caatggcacc actaagaata
     3361 gctttttcaa gcagagtcat ctgtgttttc ttccacagca caacaaagaa taagacgagt
     3421 atagaaaggt acgataggaa tcctaagata ccggcggcaa cgagccagtc gaacgctata
     3481 ttatgcgtgc ggtcaaacca tggctcctgc gtatacattt gcggatcgta gtatttgtta
     3541 aaaacaaagt taaacccttc ctgtccccac cccaaaatcg gattttcttt aacgccgtgc
     3601 cacgccacat tccacacccg aaagcgggct gttccctctt tcagtgaaat ggacgagagc
     3661 ctctcaagcg gcggaaattt ctttattatg ttcgaattgc gaagcagcaa gaccgcgccc
     3721 ccaaacacaa cgagtgcgac aattattccg atcgatattt ttcgtaatgt atttttttcc
     3781 cgagacagaa aaatgtaaag tagcgccgca acaagaagac cggccgcgag cccgagcata
     3841 gcgcctctcg tctgcgtgtg atagagaatg aaaaggtgaa gcactactac gagaccatat
     3901 atatacgaaa ctgccttatg accgcgccac cgagccatca aaaagagtgc tataaaaata
     3961 tggaagaaaa cataaatagc gagatacgtc gcatttccga aagtggcatc gagacgcacg
     4021 ccaccctgat tgattttaac agcaccagtc aattgcaaga ttccatagaa tcccatgatg
     4081 acactgacac cgagtgacgt ttgcaaaaga cgttcaaaaa atttctcggt tttaacatat
     4141 gagccaagaa ctataaagta cgcaaagaga tgaaggtgag taataagccc ttccatgcgc
     4201 tcatagtttg accagaaact tttgtacgga tttgcaccaa aaatatctgc aattgtgata
     4261 acaacgagaa aaagaccgag tgctcccgca agatatgaaa atatcgggcg atacgaacga
     4321 tcgcgcacca taagcaaaag ccaggctgca aagataattt caacaatgat gcgaaaccag
     4381 aagttttttc ccgtgataaa cggaaaaagc atatctcgag agatgataag cggaataaat
     4441 ggcacgagaa ataggcctgc tgtaacaata tattttagat atttgttcaa ccgcatcccg
     4501 ttagagaccc gcctgccatc gcctgacaca ccgatgggag gtagagtaat taattggtaa
     4561 tatcatctta attttatcaa agcgactgcg tatttcgctc tggatgtggc gggcaggtag
     4621 gaaacgcagg cttagattcg aagctcggcg cttgacctac tatttattaa taatgaaata
     4681 tctgataaaa ataaacacaa aaaaacgttt ccgtaataaa tctcttaact ttgatttgca
     4741 caatacaacc ttaactcaac ttggtcaaaa tacataacag cgagatctct aacgggacgc
     4801 atggagagaa ctataccaca ctaattttca ttaacaatat tcccgaattt cttgcgcgca
     4861 caagggccag agtgtatagt ttttaaactt ctatgctccg aggacgagat tatcgagtag
     4921 ctacggtagc tggactatta accgcagttt ttgtgtccgg aatgcttttt tttagttgga
     4981 gagaagctct gacatattcg ccaatacttt ttgttacagc accgcttata tacgttgctg
     5041 gaattagact cggatttttt ctcggaaaaa agtacaagtt tttcgagcaa tttggaagat
     5101 ttgcagtggt tggttttttt tgcgcctcga tcgatttttt tatacttaac gcactctcgg
     5161 aactaagcgg catagagcga ggttttcttg taggttgggt aaacgttccg ggtttctctg
     5221 tagcgcttgt ggtaagctat cttttaaacc acagatgggt ttttggaaac aacaatttgc
     5281 tcgatcaaaa tgagagtggg ttaaagaagg tgccaaattt gttgtctgcg tttcgacatt
     5341 tccccacatt tctcgtcatc tctgtaatcg gtttgttttt aaacagcggg atcattataa
     5401 ttcttactac atatattcca tcgcaaaact acaacgacga actcgtcctt aatgtggcca
     5461 aggttgtcgc ctccgctgtg gtaatgattt ccaattttat cggttataaa tttatcgctt
     5521 tcagaaaaaa aatggaacgc aatcctaaat gaaaaaactc tcgataatca tcccagttta
     5581 taacgaaaag atcactattg aagagcttct caaaaaagtg gagtgggcgc cgacaccagg
     5641 tctcgagaaa gaaattatta tcgtagacga ctgctccact gacggcactc gtgaaattct
     5701 agaaaaaatc ggagagcgaa tccggtacaa agttttctat cacgcaaaaa atcaaggcaa
     5761 gggccgtgcg ctgcgaacag gattcgcgag cgccacggga gatttcataa ttattcaaga
     5821 cgccgacctt gaatataatc cggaagaata tgtgaacctt ctggggcccc ttctcgacgg
     5881 aaaagctgac gtagtttatg gatcacgttt tatttcagct cttccccatc gtgtactctt
     5941 tttctggcat tttgtcggaa ataaagtcat tacaaatttc tccaacatga tgactaatct
     6001 caatcttacc gatatggaga caggctataa agctttcaac gaagaagctc tcaatctcat
     6061 aaaacataaa ctttcttcaa acagattcgg catcgaagtg gaactgaccg cgcgagttgc
     6121 ccaagcgcat ctgcgaatat tcgaggtcgg tatttcatac tccggacgta catacggtga
     6181 aggaaagaaa atcggctgga gagatggcgt cgcggcgctg tggtttatca tcaaatataa
     6241 cctcttcact taaatggaca agctcacact cacgctcgga gcactactca ttgtctataa
     6301 tgcatatctc gccggtaaca gcattatttc ccgacttaga attgctgttg ccacccgtca
     6361 aggccgcata ggatacgcca ttacgattgg gctcggcctt atcgttttta ttggatttat
     6421 actcgcaatt tttagcatat tatacgcctg gcttttatgg ctcttacttc tcatatcgct
     6481 catcatttac agaaaaaaga tttactcaca catcttgttt tttttgagat tacggctcgg
     6541 gacaggactt agtatggtta aagccgcatt acgtccatac tggtttttga aaacgcttat
     6601 cgtcatttgg cttcttggaa attttctact cgcctttgtg ccgctcaccg gtcacgacac
     6661 gctcgactac catctcccca ttattgaatc agtcgcaaat tcacatggac ttgatttcgc
     6721 ccgaagcgta gaagatttca actaccttcc tattctcgga gaaattctgt acgcaattcc
     6781 gacagtgctc ttcagtgaac atgcggcgcc gtatgtcttt cagatccttc agtatttagt
     6841 tattgttttt gtttcgctga tcgtgtggaa cactgtcctg cctagagtta aaaataaatt
     6901 atttggattt gcttctgtgc tcaccgtgct ttccctcatg gatttccaaa gagaagttct
     6961 gcacggaggc tatatcgacg ttttggggtt cctcttcgga ttggcagcac tatttttgct
     7021 catcgaccat cttgaacggg acgcgggttc aaagcgtgga tatatcttgt gcggattatt
     7081 tttgggcctt gccctatcag taaaatatac ggggctattt tttgtagcca tcagtttgct
     7141 ttttattttg attcaccact taaaacgaaa agaaactttt ctcggcctca ttcgagatgg
     7201 cctttatctt ggtataccgg ctgtcatcgt ctctggattt tggtatgtaa gaaatctatt
     7261 ttcattcggt aatccagtgt ttcccatgtg gtctgacgcg gaaacgacgg caagcgtcgg
     7321 gctctttatc atggaccgaa cggtcggcaa ttttttgtat ttcccgtttt accgatacgc
     7381 acagtggttt ctgcaggaag ttgaaacatc gagcagactt atcgtactcg gacttttcat
     7441 cgtgctctac ctcctaatca ttattctcgc gatcgccaag cggcgattca ccctcacatc
     7501 ggtcttttta ttcatttttg ttgaactgta tttgcttttt ctcttcatct catctcatca
     7561 gtaccgtttt ttgattcccg caacaattgg cgcgtcggtt cttgtcgcac tgcttgctga
     7621 ttcatttgcc agttggatcg aaaataaaat aaacactgat tctagaaaga aaatttttca
     7681 aggagtgatc tccggccttg tattggctgg cttcctttta gtctatctgg gcaaccttca
     7741 ttattttgaa gttaaatgga aatacgtcct cggaaaaacc acaacggaaa gatacattca
     7801 cgatatcggc ggacaataga aggacgctcg ggcaacttgc acgaaagtgc acggatattt
     7861 tcttgctaga aaattcctcg agcttgcgcc gtcgcgctcg ctaggcactt tcgtgcaaca
     7921 gacctcgctc taacaaaaaa agtcacgtgc tagaaattaa tgacctcctc cccgcacttg
     7981 cgtgcggggt ttcttggtaa ttaaaaaggc gccgctagta ttagccacgc ctcactccgt
     8041 acttagacgt tcaaactgac taccttgccc tcaaaccaac tatcgctgca atggggatcg
     8101 gatgtttctt gaatcgcacc ccggatttta cccatgaaag ttgaggcgag ttcgggtgcc
     8161 ggacaatccg gccccagaag caccttgagc ctccggtgga tcttccttct catttcccgc
     8221 aactgcctcg gggaaggatg ttcgcggcaa aaacgtatta ccgcgtcctt acctttattg
     8281 aaggtgaaac agatcatgag aacgtcctcc ttcttcaaat attcaggcat aaaaaccttt
     8341 cgtgttggtg gtttaaacct aatctacaac aatgaaaaga caaagtctat acggcttcac
     8401 gaatgagact cgtgcggcct tctattgctc ttttatatgg agagcgtttc tccaggttat
     8461 tgcttagaaa ttttgattat tataagtcaa gcgacgccct tggcagaatg cttatgcggc
     8521 tcaccggctg gaataaagag ccagtcgccg ggactaagga tcatttctct tcctccttta
     8581 atttcttttg ctttccattc tcgttcatct agtgatccgt ctttattcac ttttgctttt
     8641 ccgttcacca attgtccgcc gcaaatgaac gtaacttcgc cctccaaaca atgccacaag
     8701 tcagcctcgt gcatatgaac ttcagcgtca ttatcaacat ttttatcttc aagaatattg
     8761 aatggcacgc cgtttatttt tgcaaattct tttagcggtt cgagtaaccg cttcccttcc
     8821 accgattctt gtttaagaac tccttgaatg tgtgaatttt caatgtgaat ggattctcgt
     8881 ttcgacattt ggttatttta aaattgtttt taacttttcg gcgataaaaa catccgtggc
     8941 ggaacgagaa tcgctcgtca tgccgccaat atggggaacg ataatgagat ttttgttttt
     9001 cttcgcgtat tcaacaagcg gatgattttt aaaaccctct gtctcaaaat ctagttcgtc
     9061 ggaaagtaca tctgtggcat aaccggcgat ttgttttgac ttcaaagcat ctaaaatgtc
     9121 agcttcattt acgattttgc cgcgtgacgt gttgatgagg tatgcggttt tcttcatctt
     9181 agcaatagcc gctgcattga agagattttc agtactagga gccagatgga catgtattga
     9241 aataaaatcg cttttagcaa gcaatgtctg aaaatcaact tttcggtact cgtttgagtc
     9301 tacgtttgga tcgctgtata tcaccgccat tccaaacgct ttaccgtatc gtgctgtcat
     9361 cttgccaagt cttccaagtc caacaactcc aagtgttttc ccgtagagac tcactcccct
     9421 ccaatcttcc cttttccatt cgtattgttt taccgattca aaagcccacg gagtgtagcg
     9481 gacaagatct atcattaggc caagagcaag ttcagccgtg cccgtaatcg tatctagaaa
     9541 agcgttttcg ccttttagac tcaaaacttt aatccccttc gattctgcgt gaacaacatc
     9601 aatatgatcg agtcctgtgg tagcagtagc aataactttg agatttctcg cgttcgacaa
     9661 aacttcttta tcaaaattta gtcctagccc aataacagta acatcataat cagcaacaat
     9721 tttttcaagc tcgtcttgca aaggagttgc ataagtaact tctcccattt gatttaaaat
     9781 agttttcgcc tcgggcgtgt aaatttcacc tatcgtgttt aagattttta aagtcatgac
     9841 ataccaatat actaattgtt acaaatatac aaatggatac gaataagtca cgttagtatt
     9901 cattggtata ttggtactca tgcgcagatt ggtattaaaa cttgtatcc
//
LOCUS       DATDSU010000060         4425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2227786, whole genome shotgun sequence.
ACCESSION   DATDSU010000060 DATDSU010000000
VERSION     DATDSU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227786"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1448
                     /locus_tag="VI981_02105"
     CDS             480..1448
                     /locus_tag="VI981_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4479126.1"
                     /translation="MKSFSPSHYIFAIGFALLSGVAVASLFWYATTFAVERIVSNESG
                     NQISENEQSDENENTLRRGISATVIDQGMLEQIHKSLPHTFTSNVNAKSYIVAELGGR
                     TLSAQNSARVLPIASVTKIVTAYVALDLYEKTEKIKVTSDALAAYGTTGRLRAGESLL
                     LNDLLHALLLESSNDSAEVIARSYGRNSFLARMNDAAKKMGATHTYFDDPSGLSPKNV
                     STAEDLANIASYIYSRKREIFDITKVKTYRSGRHIWNNPNVLLSLSNFIGGKNGFTDE
                     AQKTGVALFSVKNGLFGEKKIAIVLLGTNEREQDISKLLRAVEENN"
     gene            1549..2529
                     /locus_tag="VI981_02110"
     CDS             1549..2529
                     /locus_tag="VI981_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY4479127.1"
                     /translation="MQGFWKNLPKPFFALAPLANVTDAAFRAVIAKYGKPHVMFTEFV
                     SADGLFMGGMEKLMFDLLFGAKERPIVAQFFTARPENMEKAAMLARELGFDGIDINMG
                     CPDRSIERQGAGAAHMKNPKLAREIIRAAKKGALDLPVSIKTRIGYNKDELESWLPEL
                     LAAEPAAITLHARTRKEMSKVPAHWNRVKDAVKIRDSLKSKTLIVGNGDTFNIVDARM
                     RATESGADGIMLGRAIFGNPWMFREYAPSLGEKLEVLVEHTELFEQLLGSVKNFAIMK
                     KHYKAYVNGWEGAKELRVKLMGAASAREVQMTVEKYLEDGNVHGRMKNEP"
     gene            3233..4327
                     /gene="dnaX"
                     /locus_tag="VI981_02115"
     CDS             3233..4327
                     /gene="dnaX"
                     /locus_tag="VI981_02115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY4479128.1"
                     /translation="MSNAHIAFYRKYRPQHFKDVAGQDHIVRVLESSITLGRISHAYL
                     FYGSRGTGKTSVARIFARALGTTSDDLYEIDAASNTGVDDVRALNESVATLPFSSKYK
                     VYIIDEVHMLSKAAFNALLKTLEEPPAHVVFVLATTEMEKLPETVVSRCQTFAFKKPS
                     VPLLSEFLQKVSKKEGVTLDKASAELIALLAEGSFRDAHGLLEKVAITAKNGKIDIGV
                     IEAIAGAPRNELVNTYLEAFSEKDATKGLGAIHEAVKANADMKFFLRLSLYKLRAILL
                     YKYAPDLRPTLMEELGAHDAAFVARMSEKKIIDSQVLLALLNADEYMEVSSVAGLPLE
                     LALIGFMEHRDAHPGTPVSKQYRPLRQAAG"
BASE COUNT         1229 a          993 c         1087 g         1116 t
ORIGIN      
        1 taatgatgtt aaactcctac aatcacgact cattaaagaa ggcctactgc ccgctgactc
       61 cagcactggc ttctatggtc ctcttacaaa acaagcagtg aagcagtatc agagcaagaa
      121 cggaatagag agtactggta ccgtaggacc actgacgagg agagcactct ctcggtaaaa
      181 acaaaatcac tctccacgca acagacctga aagttttact ttcactcgct cttcgcttgc
      241 tgatatagaa gcacttgcgt tgttcatctt atatatcgcc gtcactcgaa ctgaatgaag
      301 gtttcggctt tgctccacac ctcaatacca gttatcatag agcccataaa tgcgataata
      361 gaaaatagcc ttaaagtttc ggttattatt gttagataca gcccagaaaa taattatcac
      421 caaattaaag aagacgtggc atatatgcac cgaagtgcaa tgcaactttt caccacgcca
      481 tgaagtcgtt tagtccttct cattacatat ttgcgatcgg atttgcgctt ctttcaggtg
      541 tggcagtggc ttctcttttt tggtatgcga ccacttttgc tgttgagaga atagtttcga
      601 atgaatctgg caatcaaatc tccgaaaatg aacagtcaga cgaaaatgaa aacacacttc
      661 gacgtggtat cagcgccacc gtcatagacc agggaatgct cgagcaaatt cataagagtt
      721 tgcctcatac atttacatct aacgtaaatg ctaagagtta tattgtcgca gagttaggag
      781 gcaggacgct ttctgcccaa aatagtgccc gggttttacc gatcgcttca gtcacgaaaa
      841 tcgtaaccgc atacgtcgcg cttgatctct atgaaaaaac ggaaaaaata aaggttacca
      901 gtgacgcact cgccgcgtac ggaacgaccg gccgtttgcg cgcaggggag agcctcttgc
      961 tcaatgattt actccacgcg cttttacttg agtcaagcaa cgattccgca gaggtgattg
     1021 cgcgcagtta cggcaggaat agcttcttag cgcgaatgaa tgatgccgcg aagaaaatgg
     1081 gggcaacgca cacctatttc gacgatccaa gtggactttc tcccaaaaat gtctcgaccg
     1141 cagaggatct cgcgaacatt gcatcctaca tctatagccg caagcgggaa atatttgata
     1201 tcacgaaagt aaaaacatat cgatctggac gccacatttg gaataatccg aatgtactgc
     1261 ttagcctctc taattttatt ggaggcaaaa acggttttac cgatgaagca cagaaaacag
     1321 gggtggccct cttcagcgtc aagaatggac ttttcggaga aaaaaagatt gccattgtac
     1381 ttctcggaac aaatgaacgt gagcaagata tatcaaaact tttgcgcgcc gtagaggaaa
     1441 ataattaaac aattccgtgg taacaaaatc ggcccgtggg cagcgtcctt tttgcgtcca
     1501 aggcgcactc atgcaaaggc gtgtgcaatt gtgatagagt aatcgcttat gcagggattt
     1561 tggaaaaatt tgcctaaacc tttttttgca ttggcgccgc tcgctaatgt gactgacgct
     1621 gcctttcgcg ctgtgatcgc aaagtatggc aagccccacg taatgtttac tgaatttgtt
     1681 tctgcggacg ggctttttat gggaggcatg gaaaagttga tgttcgatct cttatttggc
     1741 gcgaaagaaa ggcctatcgt cgctcaattt tttaccgcac gcccggaaaa tatggaaaaa
     1801 gccgcaatgc ttgcgcgaga acttggtttt gacggaatag acataaacat gggctgtccg
     1861 gacaggagca tcgagcgtca gggcgccggc gcggcgcata tgaaaaaccc gaaattggcc
     1921 cgcgaaatta ttcgggccgc aaaaaaaggg gccttggatc tgcccgtttc aatcaagaca
     1981 aggatcggtt acaacaaaga cgaacttgaa tcatggctgc cggaacttct cgcggccgaa
     2041 cctgcggcga ttacgcttca cgcacggacg cgcaaagaaa tgtcaaaagt gccggcacat
     2101 tggaaccgcg taaaagatgc ggtgaaaata cgagacagcc ttaaatcgaa aactcttatt
     2161 gtcggaaacg gagatacttt taacattgtt gacgcgcgta tgcgcgccac ggagtcaggc
     2221 gcggacggca ttatgctcgg gcgagctatt tttggaaacc cgtggatgtt tcgagagtat
     2281 gctccttctc tcggggaaaa gttggaagtg ctcgttgaac atacagagct attcgagcaa
     2341 ctgcttggct cggtcaaaaa tttcgcgatt atgaaaaaac actataaagc ttatgtaaat
     2401 ggctgggaag gagccaaaga attgagagta aaactcatgg gggcggcgtc cgcgagagag
     2461 gtacaaatga ctgtggaaaa atatcttgaa gatgggaatg tacatggtag gatgaaaaac
     2521 gaaccgtagt aacccctaac cagaaaggtc ttatgcaaaa caaggactct cccgcaacgc
     2581 ttctcttttt gggggaagga ggtgatggcg cttaggcgcc gagatcgagg taggccggga
     2641 atcctacctt tgcgaatgca gatttcttat cggtcggtta atccgaacgg tcagctctgt
     2701 tgaggcaaaa aatttcagaa cattcccaag cggtccgcct aaggtgggcc gctaactttt
     2761 tctaggcgta attcagtttt cgacaaagca tacttcgtct ttagaaaaaa tgtactcggc
     2821 agtcggggac agagcgacat ttcaatacat gtgtatgcgt atgtttaaaa agacaagccg
     2881 accctatact ggggataaag aggttgtagg gatcgggcgg ctttgttatg cttaaaaaag
     2941 ttagataggg aaaggatatc ttttggcgaa acctaatttt ccaccaagga ggtggataag
     3001 acactacgtg tcatcaggct aacaaatcct acctatcgac ctgtgagcag gggggtgaac
     3061 agctgttccc cctgctcgca gcgttcctct tttttacaag agcggcccgt ggctgctttt
     3121 ttgtgtgtct ctttctctca tttatatgat tgaagtcaca tatatttgct acgctcggct
     3181 gaaatgaatg cgagccttca tttcttgctc gcttgcaatt ataatggaca ttatgtctaa
     3241 tgctcatata gcgttttata gaaaataccg gccacagcat tttaaagatg tcgcaggtca
     3301 ggatcatata gtccgtgtac ttgagagctc gattacgctc ggtcggattt cccatgcata
     3361 cctgttttat ggttctcgcg gtacgggaaa gacgtcagtg gctcgtattt ttgcgcgcgc
     3421 gctcggcaca acgagcgatg atctatacga aatagacgcc gcttcaaaca ccggagtgga
     3481 cgatgttcgg gctctcaacg aatcagtagc aacactgccg tttagctcaa aatacaaagt
     3541 ctacattatc gacgaagtgc acatgctttc caaggctgca tttaatgcgc tgctgaagac
     3601 acttgaggaa ccaccggctc acgttgtgtt tgtgttggcg acgacggaaa tggaaaaact
     3661 tcccgagacc gttgtttccc gatgccagac ctttgcgttc aaaaaaccaa gcgtgccgct
     3721 gttgtcagaa tttctgcaaa aagtttctaa aaaagaagga gtaacgcttg ataaagcgtc
     3781 tgctgaactt atcgccttgc ttgcagaagg atcttttaga gatgctcatg gacttcttga
     3841 aaaagtggcc ataacggcga agaatggaaa gattgatatt ggggtcatag aagcgattgc
     3901 cggagcgccg cgaaatgaat tggtcaacac ctatctcgaa gctttttcag agaaggatgc
     3961 caccaaaggt ctcggcgcta tccatgaagc cgtgaaagcg aatgccgata tgaaattttt
     4021 cttgcggctt tcactctaca aactgcgggc gatcttgctc tataaatatg ctccagattt
     4081 gcgcccgacc cttatggagg aattaggagc gcacgacgcg gcgtttgtgg ctcggatgtc
     4141 agagaagaaa atcatagatt ctcaagtact tttggccctt ctcaatgccg acgaatatat
     4201 ggaagtttcg agtgttgcag ggttgccgct cgaattggcg ctcataggtt ttatggagca
     4261 tcgagacgca catcctggga cgccggttag caagcaatat aggcccctgc ggcaagccgc
     4321 agggtaatac ttggtgctcg cttcgctcgc tcaagagcgt ggcaagccac gcggtattga
     4381 cctatatttt gtcttccccg cccgaacgta ccgttcggta cgggc
//
LOCUS       DATDSU010000061         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_58497, whole genome shotgun sequence.
ACCESSION   DATDSU010000061 DATDSU010000000
VERSION     DATDSU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58497"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2359)
                     /locus_tag="VI981_02120"
     CDS             complement(<1..>2359)
                     /locus_tag="VI981_02120"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY4479129.1"
                     /translation="PTPLIEYVPLQYDPKGGKIITQYDMHAVDDVGLLKFDFLGIRNL
                     SILGDAVRLAKEHRNADVNIEKIPLDDKKTFELLARGETMGLFQLGGSGMTKYLKDLK
                     PSSIHDINAMVALYRPGPIESIPAYIERKHNPQLVRYLDPRMKEILDQSHGVITYQDD
                     VLLIAIKIAGYSWLEADKLRKAMGKKIPAEMRAQKEKFTKGSIENGLPASKAEELWRL
                     IEPFAAYGFNKCVTGDSELSDAKTGEKFTVEELYNSKRLPQILSCEKDLTLRSKNITV
                     VQQNGVKPVYKITTRRGLSLKATVNHPFLKFSGWTELQNLKIGDRIATARIAPTPKGL
                     YAIEDYRLATLGYLLAEGNVCHPHGIYFYSKEKKEIRDFINYARQFPNIQTTINRSKS
                     AASVYVGKKEQKKSNELREWLKKIDLLGKNALSKKFPRFVFQLHAQQLALLIGKMWQG
                     DGCVHDGREDSMIYYATSSKSIAYDMQHLLLRLGIVSTVHRKVFSYRESKRLGWTIHV
                     SRYTNLNTFASTVGVHLIGEPKRTLEKIISNHPVLSSTLEPHGARGSKDIVPIGVLPI
                     VKEEIYKGYETLRGFAAANGISPRLLWQDRQKTGYLRETISRLGTLLHSRVLLGIANS
                     DVYWDEIVAIEQMDSEMTYDITIPDNHNFVANDILVHNSHAASYGRVAYQTAYMKANF
                     PGEYMTAVLTADSGDIEKIAEIIDETKRMGIPVLPPDVNESYAQFTLIKGLDDASDTI
                     RFGLESVKNVGTNIVQAIIEARVERGKFSSIADFAERVHHKDFNKK"
BASE COUNT          559 a          529 c          526 g          745 t
ORIGIN      
        1 attttttgtt gaagtccttg tgatggacgc gttccgcaaa atccgcgatt gacgagaatt
       61 tcccgcgctc gacccgcgcc tctatgatcg cttggactat atttgttccg acgtttttca
      121 ccgattcaag accgaaccga atcgtatcag atgcgtcatc taatccttta ataagcgtaa
      181 attgcgcata ggactcattt acgtccggcg gaagcacggg aattcccatg cgttttgttt
      241 catctatgat ctccgctatt ttttcgatgt caccggagtc cgccgtgagg accgcggtca
      301 tgtattctcc cgggaaattc gctttcatat aggcggtctg gtatgcgacg cggccgtagc
      361 ttgcggcgtg agaattgtga acaagaatat cgtttgcaac aaaattatga ttgtctggta
      421 tcgtaatgtc gtaagtcatt tcactgtcca tttgttcgat ggcaactatt tcgtcccagt
      481 acacatctga gtttgcgata ccgagtaata cgcgcgagtg caaaagcgta ccaaggcgac
      541 ttatcgtttc ccgcaaatat cccgtttttt gtctatcttg ccacaacagc cgaggagaaa
      601 taccgtttgc cgccgcaaat cctcgtaagg tttcgtaacc cttgtatatt tcttccttga
      661 caataggaag tacgccgatc ggaacaatat ctttactacc tcgtgcgcca tgtggttcaa
      721 gagtgcttga caagacaggg tgattggata taattttttc aagagttctc ttcggctcgc
      781 caataagatg aacgccgaca gtcgaagcaa atgtgttcaa atttgtatat cgcgatacat
      841 gtattgtcca accaagtcgc ttgctttctc gatacgaaaa aacttttcta tgtacggtac
      901 ttacgattcc cagacgaagg agaagatgct gcatatcgta tgcaatcgat tttgaacttg
      961 tcgcataata aatcatgctg tcctcacgac catcgtggac gcatccgtct ccctgccaca
     1021 tttttcctat aagtaatgcg agttgttgcg catgtaattg aaatacaaat cgaggaaatt
     1081 tttttgaaag tgcgtttttc ccgagtagat caattttctt tagccattca cgcagttcgt
     1141 ttgatttttt ctgctctttc tttccgacat atactgaagc cgcactttta cttcgattga
     1201 tggtggtctg gatattagga aactgtcgag cataattaat aaaatctctg atttctttct
     1261 tttcctttga atagaaataa attccgtgag ggtggcatac gtttccttcg gccagaaggt
     1321 atccaagagt tgcaagtcta tagtcctcaa tggcataaag tccttttggt gtaggcgcga
     1381 ttcgagctgt cgcgattcga tccccaatct taagattttg cagttctgtc caaccgctaa
     1441 atttgagaaa gggatgattt accgttgctt tgagtgaaag tcctctcctc gtagtgattt
     1501 tataaaccgg ttttacaccg ttttgctgaa ctacggtaat gttttttgat ctcagtgtca
     1561 gatctttttc acatgagagt atttgaggca atcgtttact attataaagc tcttcaacag
     1621 taaatttctc tcctgtttta gcatcagaaa gttccgaatc ccctgttaca catttgttaa
     1681 atccataggc ggcgaagggt tcgatcaatc tccagagttc ctccgctttc gaggcaggta
     1741 agccgttttc aatagatcct ttagtaaatt tttctttttg ggcgcgcatc tccgcaggaa
     1801 tttttttccc catggccttc cggagtttat ccgcttcgag ccatgaatat cctgcaattt
     1861 ttattgcgat caacaagacg tcgtcctggt aggtgatcac gccgtgcgac tgatcaagga
     1921 tttctttcat cctggggtcg agatagcgga caagctgcgg gttgtgtttt cgctcgatgt
     1981 aggcgggaat ggattcaatc ggtcccgggc ggtagagcgc gaccatcgcg ttaatgtcgt
     2041 ggatagaact cggctttaaa tcttttaaat atttcgtcat tcccgatcct ccaagctgga
     2101 aaagacccat ggtttctccc cgcgccaaaa gttcgaatgt ttttttgtca tccaaaggaa
     2161 ttttttctat gttgacgtct gcgtttcggt gttcttttgc gagacgcacc gcatctccaa
     2221 gtatcgagag attccggatt ccgagaaagt cgaatttgag gaggccgacg tcgtcaaccg
     2281 cgtgcatgtc gtattgcgtg ataattttcc cgccctttgg atcatattgg agcgggacat
     2341 actcgatgag cggggtggg
//
LOCUS       DATDSU010000062         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1233779, whole genome shotgun sequence.
ACCESSION   DATDSU010000062 DATDSU010000000
VERSION     DATDSU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233779"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1044
                     /locus_tag="VI981_02125"
     CDS             142..1044
                     /locus_tag="VI981_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY4479130.1"
                     /translation="MKKILYIVIAVAVLVGVLFLFKGDEEKRDVLGAQSHKEAEYLIS
                     GSKVKLENGIAESEAAPGSASKIITRYFGNEVVKDLNGDGRADIVFLLTQEAGGSGIF
                     YYVVAALNMENGYVGSQALFLGDRIALQTTERGEGKIVIVNYADRKTGEDFAVSPSLG
                     KSLWLLLDTNTMQFGEVEQNFEGEADPSKMTLGMKEWTWEKTILDNGTKVLPRTGKIF
                     TITFGAGGKFSAKTDCNSVGGAYKTEGTSITFSETVSTTMYCEGSQESVFTKMLGDSG
                     RYHFTSKGELVLDLKFDSGSVIFR"
     gene            1160..1360
                     /locus_tag="VI981_02130"
     CDS             1160..1360
                     /locus_tag="VI981_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY4479131.1"
                     /translation="MNGTIKTILADKGFGFISREGEAKDLFFHSKELKGVTFDELKVG
                     DKVTFEIVDGPKGPAATNVARA"
     gene            1440..2012
                     /locus_tag="VI981_02135"
     CDS             1440..2012
                     /locus_tag="VI981_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY4479132.1"
                     /translation="MPKISNKKETVLHVAFLRGVNVGGNRQVPMLQLKEVFEELGFKN
                     VRTVLASGNVIFESARSNSSALWNTRIKIEKKLKKTFGFEIGVHLRTMNQVVKMLHAK
                     PFKNTKITPETRLYVTFVQEGTKNKIKIPHISPDKSLQILKITGEEVLSILDLEKNGT
                     ADAMNMLQKLFGKNITTRNWNTIVKLAVES"
BASE COUNT          700 a          398 c          528 g          549 t
ORIGIN      
        1 catattgtgc ttcaaatggg acggcagaag tggcggcgaa tacggttacg actctcgatg
       61 ttttctgcga ttctggaatt agataaaata gcatgtaaga atttactctt aagcacgcca
      121 ctgtggcact aacttaacaa aatgaaaaag atactatata ttgtcatcgc cgtggcggtt
      181 ctcgtcggag ttttgttctt gtttaaaggt gatgaagaaa agcgagatgt acttggcgca
      241 caaagccaca aggaagccga atatttgatc agtggttcca aggtgaaact cgaaaacgga
      301 atagccgaat ctgaagcggc tccgggttct gcatcaaaaa tcataacgcg ttattttgga
      361 aatgaagtgg tgaaagatct aaatggcgat gggcgggcag atattgtctt tttgcttaca
      421 caagaagccg gcggaagcgg catcttctat tatgttgtcg cggcgttgaa catggaaaac
      481 gggtatgtcg gttcgcaagc tttattttta ggtgatcgta ttgcgctgca gacgaccgaa
      541 agaggtgaag gaaaaattgt catagtcaat tatgctgatc gaaaaacggg tgaagatttt
      601 gcggtttccc cgtctctcgg gaagagcctc tggctactgc ttgatacaaa tacgatgcaa
      661 tttggcgaag tcgaacaaaa ttttgagggt gaagcggatc cgtcgaaaat gacgcttggc
      721 atgaaagaat ggacgtggga aaaaacaatt ctcgataacg gtactaaagt tctgccccgg
      781 accggaaaaa tatttactat tacttttggg gcgggcggaa agttttccgc caaaacagat
      841 tgcaactcgg ttggcggcgc gtataaaaca gagggtacgt ccatcacatt tagcgaaacg
      901 gtctcaacaa caatgtattg tgagggttcg caggagagtg tttttaccaa aatgctcggc
      961 gacagcggta gatatcactt tacgtcgaaa ggggagctcg tgcttgatct caaatttgac
     1021 agcggatctg ttatttttcg ataaaaacaa aaagcgattt ctgctggacg aaatcatgta
     1081 tatagggcat aatgaactct tatatatcaa aaggacacaa agtcgagtct cccaggtata
     1141 ggaattgcat tagaatcaca tgaacggaac catcaagacg attcttgcgg ataaaggatt
     1201 tggattcatt tctcgcgaag gcgaagcaaa ggatctattc tttcattcaa aagagttgaa
     1261 gggtgtgaca ttcgatgagc taaaagtggg cgacaaggtc acttttgaga tcgtcgacgg
     1321 tcccaagggt ccagctgcga caaacgtcgc acgggcataa gagagaaaac atccctccgt
     1381 cggcaagacg gagggatgtt ttctatctca agataaatat ttttgcaaag tcaagacaaa
     1441 tgccgaaaat atccaataaa aaagaaactg tgttgcatgt agccttcctt cgaggagtaa
     1501 atgtgggggg caataggcag gtgcctatgc tccagcttaa agaagtattc gaagaactcg
     1561 gcttcaaaaa tgttcgaact gttttagctt ccggaaacgt aatatttgag tccgcgagga
     1621 gtaattctag tgcactgtgg aatacgagaa tcaagattga aaaaaaatta aaaaaaacat
     1681 tcggatttga aatcggcgtg catctacgga caatgaacca agttgtgaaa atgctacacg
     1741 cgaaaccatt taaaaatacg aaaattactc cggagacgcg tctgtatgtc acatttgtcc
     1801 aagaagggac aaaaaacaaa attaagatac cgcatatttc tcccgacaaa tcattgcaaa
     1861 tattaaaaat caccggtgag gaagtgttga gcatactgga tctcgaaaaa aacggtacag
     1921 ccgatgccat gaatatgctt cagaaactat tcggcaaaaa tatcacgacg cgaaattgga
     1981 acactattgt taagttagcc gtggagtcgt agacaacgta agatcgagtg gtcttctcga
     2041 taaattttag tatactcaaa gtatgcagag tgatttcgcg atcgaaataa aaggcctgac
     2101 taaatcgttt aaaaaagtca gagttcttga aggaataaat ctgaacgtca aacgtggcac
     2161 gatgcttgcg cttct
//
LOCUS       DATDSU010000063         4006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_566170, whole genome shotgun sequence.
ACCESSION   DATDSU010000063 DATDSU010000000
VERSION     DATDSU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566170"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..349
                     /locus_tag="VI981_02140"
     CDS             53..349
                     /locus_tag="VI981_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479133.1"
                     /translation="MSVRLFRTKGLERELQPSHVIRGISIWKEQQNGEPIEMILSREV
                     SPPALIPVEQHRRLVGERKRRNSEYYKRNPNALRLKIHGPRLTGDRVGTCFVRF"
     gene            595..1182
                     /locus_tag="VI981_02145"
     CDS             595..1182
                     /locus_tag="VI981_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479134.1"
                     /translation="MNEFTDIHPSLAALYASSYAPATTRLSSDDYFVCFPVGKESTAT
                     FTGEPREFGGFLCLTHVDLLLGSYLMLGLVPHVAMKCYRSKLERLAREVEKGSFQFPE
                     RHTLEFQNRHAVTISWDPVSHLPTELGELLYFGIPVGRLVDELLGFAGREPLFLYSDL
                     LPPAFAVIFLERWRSLPAMLRGEVRSGSHEPALTR"
     gene            1304..3367
                     /gene="uvrB"
                     /locus_tag="VI981_02150"
     CDS             1304..3367
                     /gene="uvrB"
                     /locus_tag="VI981_02150"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY4479135.1"
                     /translation="MSVFKLKTAYTPAGDQPKAIRSLLAGLEKKERHQTLLGVTGSGK
                     TFTAANVIEAFQKPTLVIAHNKTLAAQLAQEYREYFPTNAVHYFVSYYDFYQPEAYLP
                     VTDTYIEKEAQINEEIDRLRHASTQALLTRTDVIIVATVSCIYGLGSPVEYEKVNLRI
                     EVGERATRRDLLRRLIAIHYDRTSADLSPGLFRAVGNAVEIMPVNERTNYRVQFEGDF
                     VSAILKIDPITSVLIEEIENFFLFPAKHFITDKEQSARALETIKSELDARLEELKTAG
                     KILEAERLKRRTNYDLALIREIGYCNGIENYSRHFSGKAQGEPPDTLLSYFPKTEDGA
                     PDFLTVIDESHVTVPQLNGMFAGDASRKENLVEHGFRLPSAKDNRPLKFPEFMERVGA
                     IIYTSATPGKYELEHSKRIAEQVIRPTGLVDPKVEVKPLLSKAEYKGQIFDFIDEALT
                     AIKKGGRVIATTLTKKMAEDLSEYLKEKKVKAEYLHSEIATIDRIQILTEFRKGKFDV
                     LVGVNLLREGLDLPEVVLIGILDADKEGFLRSETSLIQTIGRAARNIDGRVILYADHI
                     TGSIERAVSETNRRRTLQLEYNKKNGITPKTIVKRIKDITEEMQSEHQKTVKTLLEFD
                     LKLFEKNPQKLIKDKEKQMNNAVKILDFESAAILRDEIRMLEKKLEKTTRENRKPAQG
                     KNKNV"
BASE COUNT         1119 a          870 c          972 g         1045 t
ORIGIN      
        1 cacgccggaa gagaacgaat gagtacaacc aaaaaacaaa aaggacatac acatgagcgt
       61 cagactgttt agaacaaaag ggttggaaag ggaattgcaa ccgtctcacg ttatcagggg
      121 aataagtatt tggaaggagc agcaaaatgg agagccgatc gagatgattt taagtaggga
      181 agtctctccc cccgccctga ttcctgttga gcagcaccgt cgtctcgttg gtgagcgaaa
      241 acgtcgaaat tcggagtatt acaagagaaa cccgaatgcc cttcgcctga agattcatgg
      301 tccgcgtctt actggagatc gtgtcggaac atgttttgtg aggttctgaa ttatgtaccg
      361 ccttattttg ggcggttttt tcttttagaa aagaccttgc acataaaccc cgttagaagc
      421 tcacaggggg cattaaaccc gtgttcgcta aaattaaatt gcattctgct tctaacgggg
      481 taaacgtaag gaggaggcat tgggctccat aaaatgttcc gacgttggga tatttcgaga
      541 cttatggtat tttgtatttt agaccatcta accaaacgta aaacaggtaa ccttatgaac
      601 gaatttactg acattcaccc ctcactcgct gcgctctatg cgtcttcata tgctccggcg
      661 acgacccgct tgtcttctga cgactatttt gtctgttttc ctgtcggtaa ggaatcgacg
      721 gcaacgttta ccggggaacc tagagagttc ggaggctttc tctgtttgac tcatgtggat
      781 cttttgctgg gttcgtatct tatgctcggt cttgttcctc atgttgccat gaaatgctac
      841 cgatcaaagc ttgaacgcct ggcgcgagaa gtcgaaaaag gaagttttca attcccagaa
      901 aggcatacgc tcgagttcca aaaccgacat gcagtcacaa tttcttggga ccctgtgtcg
      961 catctgccaa cggaactcgg agaattgctg tactttggga ttccggtcgg gaggctggtt
     1021 gatgagcttc tgggtttcgc cggccgtgag cctctgtttc tttattcgga tttattaccc
     1081 cctgcatttg ccgtgatctt tcttgaacgc tggcgctcat tgccggccat gcttcgtgga
     1141 gaagttcggt ctggcagtca tgaaccagct ctgacgcgct aaacgtgcgt cttttttaat
     1201 aaataaacga gcgaagcgcg aattttgaag tgcagtctcg caaaatttag tgtcggggtt
     1261 gttctaattt ttaaaatatc tcgcgaaagg gtatagtgtt tgaatgtcag tttttaaact
     1321 caaaaccgca tatacgccgg ctggagacca accgaaagct atacgttcgt tactcgcggg
     1381 cctcgagaaa aaagaacgac accagacact tctgggtgtt accggttcag gaaaaacatt
     1441 tacagcggct aatgttattg aggcctttca aaaacccacg cttgttattg cacacaataa
     1501 gacgcttgcg gcgcaactgg ctcaagaata tcgtgaatat tttcctacta atgctgttca
     1561 ttatttcgtc tcctattatg atttttatca gcctgaagca tatttacctg tcactgacac
     1621 gtacatagaa aaagaggcgc aaattaacga ggagattgat cgtttgcggc atgcctcaac
     1681 ccaagctctt ctcactcgta cggatgtcat tatcgtagct acggtctcgt gcatctatgg
     1741 tttgggcagt cccgttgaat atgaaaaagt aaatttgcgt atcgaagtag gcgagagggc
     1801 aacgagacga gatttattga gaaggctaat tgccattcac tatgaccgca ccagtgccga
     1861 cttaagccca ggacttttcc gtgccgtcgg caacgctgtc gaaataatgc ccgtgaatga
     1921 gcgcacgaac taccgagttc agtttgaagg cgactttgtt tcggcgattc tgaagattga
     1981 ccccattacg agcgtactca tagaagaaat agaaaatttt ttccttttcc cggccaagca
     2041 ctttattacg gacaaagaac aatcggcccg ggcattggag acaattaaat ctgaacttga
     2101 cgcgaggctt gaagaactca aaactgcagg gaaaatcctt gaagccgagc ggctgaaacg
     2161 ccggacaaat tacgaccttg cccttattcg tgaaatcggg tattgcaacg gaatcgaaaa
     2221 ttattcgcgt catttttcag gaaaggcgca gggagaacct cctgatacgc ttctttcgta
     2281 tttccctaaa acagaagacg gtgcccctga ttttttgacc gttattgacg agtcgcacgt
     2341 cactgtgccc caactaaacg gcatgtttgc gggtgatgca tcgcgaaagg aaaatttggt
     2401 ggaacacggt ttcagacttc catctgcgaa agataatcgg ccgctcaaat tccccgaatt
     2461 tatggaacga gtcggagcga ttatttacac gtcagcaacc ccgggtaaat acgagctaga
     2521 gcactcaaag cgtattgcag agcaggtcat tcgcccgact gggcttgtgg atccaaaagt
     2581 tgaagtaaag cccctgcttt cgaaagccga atacaagggg caaatatttg attttataga
     2641 cgaagcgctt acggctatta aaaaaggagg ccgtgttatc gccacaactc tgactaaaaa
     2701 aatggctgaa gatttaagtg aatatttgaa agaaaaaaaa gttaaagctg aatatcttca
     2761 cagcgaaatt gcgacaatcg atagaattca aattttgacc gaatttagaa aagggaaatt
     2821 tgacgttttg gtcggggtta atttgcttcg ggaaggactc gatttgcctg aagttgtgct
     2881 cattggaatt ttggatgcgg acaaggaagg ttttttacga tccgaaacgt cgcttatcca
     2941 aactatcggc cgggctgcac gaaatataga cggcagggtg atcttatatg cggatcacat
     3001 cactggttca attgaacgag cggtttcaga gacaaaccga agacgaactc tgcaactcga
     3061 gtacaacaaa aagaatggca ttacgccgaa aacaatagta aaacgtatca aagatattac
     3121 cgaggagatg caatcggagc atcaaaaaac agtaaaaact cttcttgagt ttgatctcaa
     3181 actgttcgaa aaaaatccac agaagctgat taaagacaag gaaaaacaga tgaataatgc
     3241 ggtgaaaatt ttggattttg aatccgcggc gattttacga gacgaaatcc gtatgcttga
     3301 gaagaaattg gaaaagacaa caagagaaaa tcgaaaaccg gcgcaaggaa aaaataagaa
     3361 cgtttgacgc ttacgttttt tggtgataat ttataagtgt tggctggact acgcgaaggc
     3421 gtcactaaag tccagcactg gctccttcga cggctgatgg ttgcggatgc gcagtttcag
     3481 cgttctacac cggttgtgaa cgtgagaagc gaagtgtctg ctcggatccg tgcggctgtc
     3541 gcgagcctgc aacgccgccc tgcacggggc gtgcgacggt agctcctgcc gtcaactctc
     3601 cggttttcgg agagtgaagt aataggagta caccgtcctt ctcgcgcacg taagtgcgtg
     3661 agcggcacgg agggcgtggg tggtgagcaa tggatgcccc tacccacgcc caaattattt
     3721 taataagact tctatgaaat cggtgaaaat tattgacaaa gtttcaatac ttagccatga
     3781 tcacacgtag aaagtttgat ttcctagcac atgtgtgcgg tcaaactgtg aaccctgatt
     3841 cttatgaagt ttcctcgcaa cattcaccgg tttgtgctct gttggattgc cgacaaatta
     3901 ctgctatttg cttctccgcg tcttgcggcc aggcactacg tcaacagttt accgctcgat
     3961 gaccaaaaga gggttgctct atctgggatg ggcgagctca atcttg
//
LOCUS       DATDSU010000064        10304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_724750, whole genome shotgun sequence.
ACCESSION   DATDSU010000064 DATDSU010000000
VERSION     DATDSU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10304
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724750"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3021
                     /locus_tag="VI981_02155"
     rRNA            <1..3021
                     /locus_tag="VI981_02155"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3043..3765
                     /locus_tag="VI981_02160"
     CDS             3043..3765
                     /locus_tag="VI981_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY4479136.1"
                     /translation="MHSLEIEERKKHIRISDFERDIIVGKLLGDGHLETKNQGRTYRL
                     KVEHSIKQKEYVDWVYGKLRHLAMSEPCVKEKDVFPPQGGTFKFHGYGFTSLSLGQLR
                     FYGQQFYNHGKKVIPKMIGKMLSPSVIAIWYLDDGSLKSNTHRTYIIHTYGYTKSDLR
                     RVQDALLKRFDIHTNLHRQNKNGKVYWRMYVMSESAWTFQKLIEPIIKHIPSMQYKLG
                     NTHAQKVTEGLKGWLRTNGNRP"
     gene            4986..5210
                     /locus_tag="VI981_02165"
     CDS             4986..5210
                     /locus_tag="VI981_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479137.1"
                     /translation="MSQEAQKPEPKFERGKDKVVSGYRRHILTEVAQKNHERADLLEK
                     KRKILEKLSPVERDMLEKIDMELNALRKEE"
     gene            5264..5443
                     /locus_tag="VI981_02170"
     CDS             5264..5443
                     /locus_tag="VI981_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479138.1"
                     /translation="MPEAMPMPQKGVNPEKLKKLEEAKKALEGFKREGWPAEVVEEEE
                     AGVKALEDDIKKEQK"
     gene            5474..5591
                     /gene="rrf"
                     /locus_tag="VI981_02175"
     rRNA            5474..5591
                     /gene="rrf"
                     /locus_tag="VI981_02175"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5979..6539
                     /locus_tag="VI981_02180"
     CDS             5979..6539
                     /locus_tag="VI981_02180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4479139.1"
                     /translation="MLTGKKVRLRAIDIKKDAELFTEWINDYEVVKFLGRPFKSISPD
                     EERRILKGLLLKKDTVLFAVETLDGGNLIGSISLMGINHFDGTAITGTLIGDKQYCRK
                     GFATDAKMVLLNYAFTVLNLRRISTRVVADNIASVRVQEKCGYTREGVWRKEVYTDGK
                     YRDVVLLAVFRNGWMKQWREYIRKAD"
     gene            6682..7362
                     /locus_tag="VI981_02185"
     CDS             6682..7362
                     /locus_tag="VI981_02185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4479140.1"
                     /translation="MSIHDGTYQLHEGAYGEFKFYQKIAKESGGPVLDLATGGGRICI
                     TLAKAGITCVGMDASPHSLVIAKKAVAELPQELRGKASFIEGDMCDFNFPGRFPLIIN
                     AFESFWFILRMAAIKDLGINEYEWWLGNEQQKDNEVFMLIYQKAESCIRSIMRSLAPR
                     GKFIIDGPISHLLFQEEKKEVAHAWWLDMADTYKFSVRFADYNDLNKSVFDLNALHRP
                     DVVIGQKI"
     gene            complement(7489..7617)
                     /locus_tag="VI981_02190"
     CDS             complement(7489..7617)
                     /locus_tag="VI981_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479141.1"
                     /translation="MSEYLGTLILAVPFALFIGIPLILNLCEKKESFNPIDNPAEN"
     gene            7761..8465
                     /locus_tag="VI981_02195"
     CDS             7761..8465
                     /locus_tag="VI981_02195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4479142.1"
                     /translation="MKPEIPSAYSAEKTKNSNGYLSDTLRPTERRKGEFAFWVREVQH
                     SGGPVLELASGAGRILTVLAEAGVDVYGLEASSEMIELGKRAMHALPVETKQHIRVVQ
                     ADMRVFTFVRKFPLVIIPFTSFWYNFKNAAIDDIGWDFETSEKSEKEFQEHIERQAEN
                     CLSSIVGALAPSGRFIIDNPFYTTHWQKENGRDKRDHCRRWWSRMVERFGFDVYIRKP
                     FDFPDDIIPVLEGIKK"
     gene            8574..8762
                     /locus_tag="VI981_02200"
     CDS             8574..8762
                     /locus_tag="VI981_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479143.1"
                     /translation="MKWKHIPADPSSPSPFDRVADYYIVEDYDMAAIPKPSPEFLREV
                     EKSDEETKEIFRKLGIIK"
     gene            complement(8795..9088)
                     /locus_tag="VI981_02205"
     CDS             complement(8795..9088)
                     /locus_tag="VI981_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479144.1"
                     /translation="MTTGQYLFLVKAYRHLLESRLIPKSEAPHDHPCYSKRTAMMHCR
                     AMLDEMENLILADEREKAMRWLGFVQAILWQNECFTLDELKGHNRSGKEPEKK"
     gene            complement(9214..9624)
                     /locus_tag="VI981_02210"
     CDS             complement(9214..9624)
                     /locus_tag="VI981_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479145.1"
                     /translation="MDTPERPELVDTDELVSLTKSFEAGKTAEKEKNSRDRQARVDAI
                     TEKITPDIHKTIGPKTREAAAEGRNSVVISSFTSNGDPEYLDGWRQACRHIAGDYRKA
                     QSKLRLETREHEHSPGSDEYYRPYTEYQFTATWG"
     gene            complement(10003..>10304)
                     /locus_tag="VI981_02215"
     CDS             complement(10003..>10304)
                     /locus_tag="VI981_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY4479146.1"
                     /translation="GGACEFSRATSHENPYVELEGVKYFGIPNIPGGVGERSAPIFSE
                     SVKPFMLESIRHGFVGALKECPALRNSVSIYEGTVTSKGLAKTFGLEYTPIDTLL"
BASE COUNT         3017 a         2067 c         2551 g         2669 t
ORIGIN      
        1 ggagcgtacc cgctgaagtg aaacatctca gtaagcggag gaaaagaaac caagtttagt
       61 tccaaataca tgcgccacac atgtacgcgt gtatttgtga actaaatcat tccccaagta
      121 gcggcgagcg aaacgggaac agcctagatc gtttgataca agggagcaat cttttgcatc
      181 ggacgaagtt acaagattat gatgtcgatt caattcgaga gggcaaaaaa tcgaatgtgt
      241 taggagaacg gctctggaaa ggccggccaa agagggtaat agccccgtat tcgaaaatat
      301 gttcgtgctc tttttcataa ctcttaagta ctgccccgtt tgtagtctgg gcaggaagtc
      361 gggagaacta gtctcccaag gctaaatact cgatatcacc gatagtgaac cagtaccgtg
      421 agggaaaggt gaaaagcagc ccggtgaggg cgatgaaata gtacctgaaa ccatacgttt
      481 acaaggagtc ggagtccgtt gcccgcaagg gcagcaggac gacggcgtgc ctattgaaga
      541 atgagccaac gagtttttgg attctactcg tctaagtgcc tttaaagcac caaggcagag
      601 ggaaaccgag tgttaatagc gcgatctaat ttttacgttc atgcgctaac gcgtatgaac
      661 gtataaatta aaagtagaat tcaaaagacc cgaaacctcg tgagcttacc atgagcaggt
      721 tgaactccga tgaaaatcgg agggaggacc gaaccggttg gtcgtacaac gccttcggat
      781 gacttgtggt aaggagtgaa aagctaatcg aacgaggaaa tagctggttc tctccgaaat
      841 agtttttgga ctagcctcgg atcacacttt tgaggaggta aagcactgga aggtcagtaa
      901 ttgtagaaat acaggctgac ctatcaaact ctgaatgcct caaattttat tccgggagtc
      961 agtacgcggg ggctaagctc cgcggtacat gaggggaaga gcccagaccg tcaactaagg
     1021 tccctaaatc tatgctaagt ggcaaaggtg gtgaggtctc tcaaacaaca aggaggttgg
     1081 cttagaagca gccatccttt aaagagtgcg taacagctca cttgtcaaga gatcgtgcgc
     1141 ttaaaattta acggggctaa gcatagtccc gacgcactat ctttcattag atagtgtagt
     1201 cgggatgccg acttcaacat tattaattta ttaatagtgc gtcggcatac cgaagttacg
     1261 ggtttgttcc gcgttcgcgc gggacaagcg gtaggagagc atttcaaatg ctgtgaaggt
     1321 tcacccgcga gggggactgg agcgtttgga agagagaatg ttggcacaag taaccacaag
     1381 cagggtgaga acccctgccg ccgaaaaccc aaggtttcct tggcaatgcc aatcatccaa
     1441 ggtttagtcg gccctaaggc aatggcgaaa gccgaagccg atggatagct ggttaatatt
     1501 ccagcactgt ggtgcatatc gatggcgggt cggagcgaag tatgtcgggc gtattattgg
     1561 atttacgttt gcggcgtgag gatggagcgt aggcaaatcc gcgctcaagc tctttaatga
     1621 gcgccgtcca agcgtcatga aaatcatgcg gagcaatcca aatgaaattc gataaagctc
     1681 gcttccaaga aaagcgtcta agattatatg catcatgacc gtaccggaaa ccgacacagg
     1741 tgggttggtc gagtagacta aggcgaacga gtgagtcttc gttaaggaac taggcaaaaa
     1801 agcagctgta agttcgcaat aaagcttacc agcctcactt tttggacata aaaaaaccaa
     1861 ctgttttagt tggcgtgaag aactctatat tttctaatct ttaaagcaat aaccgcaaga
     1921 atgatgaatg gagggtagaa gacaagaatg gaaaacaatc ctttaagcag aagtccagta
     1981 ataatatatg cggttttggg accaaaaaga tcagtgaata cgaaattcag aattccgata
     2041 caagggagcg atataatgaa gatgataatt gatgcccaaa atccgacaag tattgagccc
     2101 acagtaaacc tccaactgta tgcggaatct gaaagggatt atacaaagca atataaaaaa
     2161 agtcaagtgt gtctaaaaag tgaggctggt cgcagcgaaa gtacctctgg cgactgttta
     2221 ccaaaaacac aggtccctgc taactcgtaa gaggatgtat aggggctgac gcctgaccga
     2281 tgcgagaagg tcaaacattt ccgtagtgga gttaagatta catcaaaatt ttattgcatg
     2341 agatgtcaag ccctcgtcaa tgtcagcggt aactataacc gttctaaggt agcgcaattc
     2401 ctttccgggt aagttccgga gcgcacgaaa ggcgtaacga tcggaggact gtctcaacga
     2461 agagctcggt gaaaatacaa tgccggtgaa gatgccggct acctgcagtt agacgaaaag
     2521 accccgggag ctttactgta gcttgatatt gggtctaggt ttttgttgcg tagcataggt
     2581 gggagaggtt gatccccggt ttccggatcg gggggactcg tcagtgaaat accactcttt
     2641 aaaaacttag attctaacca atccgatacc taaaaatgga aagcggattg agacagtgtc
     2701 tggcgggcag tttgtttggg gcgaaaccct taagaagact ggtcattaag ccgatcagtt
     2761 gtgggggctc cgcgtaatgc ggagaaaaca ttcagctata tgctggaaca tccggatgta
     2821 tatgttggta cccgtcgaag ggtaaaaaac caactttgaa tatttcgcgc ttctgcggta
     2881 acgcagtatg tacgaaatgt gccggacaat cagcaggcaa ccgagcgcct atgggtgctt
     2941 gggagccctc aacgactata cgctgaaacc gctgtatggc agcggcatga tatagtctga
     3001 tctttcgggc gaccgaaagt gcgcatactt gcgcaactta atatgcattc attggaaatc
     3061 gaagagcgaa agaaacacat acgaataagc gatttcgaga gagatattat tgtgggcaaa
     3121 cttcttggtg acggccacct tgaaacaaag aaccaaggca gaacgtatcg gctgaaagtg
     3181 gagcattcaa tcaagcaaaa ggaatacgtt gattgggtct acggaaaatt acggcacttg
     3241 gcgatgtcag agccgtgtgt gaaagaaaaa gatgtatttc caccgcaagg cggaacgttc
     3301 aaatttcacg gatacggttt taccagtttg tcgcttggcc aattacgttt ttatggccag
     3361 caattctata atcatggcaa gaaagttatt ccgaagatga ttggaaaaat gctttctcca
     3421 tcggtgattg ccatttggta tcttgatgac ggatcgctta aatcgaatac ccatcgaaca
     3481 tacattatcc atacctatgg atatacgaaa tctgatttgc ggagagtgca ggatgcactc
     3541 ctgaagcgct ttgacataca cacaaatctt catcgtcaaa ataagaacgg aaaagtatac
     3601 tggagaatgt atgtcatgtc ggagtcggct tggacatttc agaaattgat cgagccgatt
     3661 atcaaacaca ttccatcaat gcaatataaa ttagggaaca cacatgccca aaaagtaacg
     3721 gaggggctta aaggttggct taggacgaat ggaaaccgtc catgacgtgt aaaggcacaa
     3781 gccagcttaa ctctaaggcg gacatgccga ggagatacga aagtataact tagtgatccg
     3841 atggctatag tacttattta cattcagggg taactctgaa tgtgagtatg tatttcttac
     3901 tagaataagg ccaaagctca acggataaaa gctaccccgg ggataacagg ctagtcgctt
     3961 ccaagagtcc atatcgacga agcggatcgg cacctcgatg tcggctcacc ctatcctggg
     4021 actggagaag gccccaaagg tttggctgtt cgccaattaa aagggtacgc gagctgggtt
     4081 caaaccgtta agaaacgaga tttatgaatc ttgatttatg atttaagact tatgatcagt
     4141 tttttaacag tttgaacccg aggaatgaca tcaataacgg cggcgttccg cagtaacgcg
     4201 gaatgtgaag ttaacttata tcggtaaaat ccttttgaca ttttccataa atgtttctag
     4261 agacaatacc gagggaagaa aaacattact atctttaaac cacaaaaatt atggcaatcg
     4321 aaaacattga aagtcgccta aaggccctca taaaagaagc gaatgaggga agaataaaag
     4381 actagatggt aatgttttat cacccgtaga gactacacgt taacctccca atgaaaattt
     4441 gcatccgcaa attgaatcgg gatgatgata tagtccaatg cacatagtaa tatgtggtag
     4501 acatgcgtga gacaggttgg tctcctatct actgcaggcg caagatactt gagaggagtc
     4561 gtcctttgta cgagaggatc aggatgaacg aacctctggt ctatcggctg ttccgccagg
     4621 agcactgccg agtagctatg ttcggaaggg ataaccgctg aaagcatcta agtgggaagc
     4681 ccacctcaag ataaggtatc atagatccgt gagagatgat cacgttgata ggccgtaggt
     4741 gtacgcgcag taatgcgttc agccgagcgg tactaataga tcatttttta cctgctctat
     4801 actaagtttg gtaagtaaga atatactata attttttcaa cgcctctgtt ttgcttttat
     4861 atttctgatc ccaccgccag cccgattgaa aaaattatag tatattcttg atggggatgg
     4921 gggattgctg aaatgaatag tcagatagta ggtttttgct attaggggaa taatcattta
     4981 attttatgtc gcaagaagca caaaaaccag agccaaagtt cgaaaggggc aaggataagg
     5041 tcgtctccgg ttataggagg catatattga ctgaagttgc tcaaaaaaat catgagcgcg
     5101 ccgatttact tgagaaaaaa aggaaaattc ttgaaaaact ttctccggtg gagcgagaca
     5161 tgcttgagaa aattgacatg gaactgaatg cgttgagaaa ggaggaataa atactatatt
     5221 gaaggaagat ttgcaaaatc ttaaaaatta acaaaaaaac tttatgccag aagcaatgcc
     5281 gatgccccaa aaaggcgtca atccggaaaa attaaagaag ctagaagaag caaaaaaggc
     5341 gcttgagggt tttaagcgtg agggctggcc ggccgaagtg gttgaggagg aagaggccgg
     5401 agtgaaggcg cttgaagacg atattaaaaa agagcaaaaa taggcctgag atttctttca
     5461 aaaattttga tttgctcgtg tttaatctat gtgtgcacca cctcttccca ttccgaacag
     5521 agaagtgaaa gcacttagat ccgatgatac ttggtgtaaa tcaacgcccg ggaaagtagg
     5581 atacgcgggc atatcaaaat ttttgaaagg aatgaaaaag ctatggaata cataattttc
     5641 acatttttaa aagaaatggc gccgcgagac tatcgcgggg tttggcatat ttctttttgc
     5701 cgcggaaatt atgatttgaa attatttttt taagttttta gtattttttc gaaacataat
     5761 tcccgcggca aactgtcgcg ggaattttac ttatagctcc ttaacaaaaa ggaggggtat
     5821 gatgaaaaaa acagtagaga atgcattacc gtttactaac ggcaagtttg agttgaatca
     5881 ggaacagatg ggaaaagcga aagcgtggct tctggagcat acgaaaacat gcacttgagc
     5941 cttgatgacg attcgtatga cggaattggg aggtagcaat gctcaccggc aaaaaagtac
     6001 ggcttcgtgc gatagacata aaaaaagacg cagaactctt caccgaatgg ataaatgatt
     6061 atgaggtggt gaaatttctt gggcgtccgt ttaagtcgat tagtccggat gaagaaaggc
     6121 gaattctcaa aggactgctt ctcaagaaag atactgtgct ttttgccgtc gaaacgcttg
     6181 acggcgggaa cctcatcggg tcaatttctc ttatgggaat taatcatttt gacggaactg
     6241 caattacggg aacgctgata ggggacaaac agtactgccg aaagggtttt gcaacagatg
     6301 cgaaaatggt gttgctcaat tacgctttta cggttctcaa tcttcggcgt atttcgacgc
     6361 gggttgtcgc agataacatt gcgagtgtcc gcgttcagga aaaatgcgga tacacgcgcg
     6421 aaggggtatg gagaaaagaa gtctatactg acggtaagta tcgcgatgta gttcttcttg
     6481 cggtatttcg taatggatgg atgaaacaat ggcgggaata tatacgaaaa gcggactaat
     6541 ttccgctttt tgatttgata aagtaattta aaagtgctac tacttattca ctgatctttt
     6601 aaacaccaag gatcgccagc agtgatttcc tccgcttatt ctgccgaaaa agttgtatcc
     6661 tgcggtaacg gccacttcag aatgtctatc catgacggta catatcaact gcacgaagga
     6721 gcatacggag aatttaaatt ctatcaaaaa attgcaaagg agtccggcgg acccgttctt
     6781 gatcttgcaa cgggcggcgg ccgcatctgc attacattgg caaaagcagg aattacatgt
     6841 gtagggatgg acgcatctcc ccatagtctc gtgattgcaa aaaaggcagt agctgaactc
     6901 ccacaggagc ttagaggaaa agcatcattt atcgaagggg atatgtgcga ttttaatttt
     6961 cccgggcgct ttcctctcat cataaatgcg tttgaaagtt tttggtttat tttaaggatg
     7021 gcggcaataa aagaccttgg tataaacgag tacgagtggt ggctaggcaa cgaacagcaa
     7081 aaagataatg aagtattcat gctcatctac cagaaggcgg agtcttgcat tcgctcgatt
     7141 atgcgttccc tcgcgcctcg cggaaaattc ataattgatg gtcccatctc gcatttactt
     7201 tttcaggaag agaaaaaaga agttgcgcat gcgtggtggt tagacatggc ggatacgtat
     7261 aaattttcag tgcgatttgc tgactataat gatttaaata aaagcgtttt tgatttaaat
     7321 gcgcttcaca ggcccgatgt agtaatagga caaaagatat aggcagttca cacttctttg
     7381 tcccggcaat aatttataaa gagcggacta cccgctcttt tttgttcgtg cggtagtttt
     7441 gttaccataa aaaagaggcc atgcggcctc ggaatggttc ggccgaagtt aattttcagc
     7501 cggattatcg attgggttga atgattcttt tttctcgcac aaattcagga taagcggaat
     7561 gccgataaat agagcaaatg gaactgcaag aattaaggtg ccgaggtatt cactcatagg
     7621 tgctccaggc ggtattgcgt gggtaggaca tgctaatctt actctatttg cctgtttttg
     7681 tcaataaaaa aatatttttt gattttatca tttattcatg gcacagttat tacaagaact
     7741 ttgaaacaag gggcaggcaa atgaagcccg aaatcccgtc ggcatattct gcggaaaaaa
     7801 caaagaattc aaacggctat cttagcgaca cgcttcggcc gactgaaaga aggaaaggag
     7861 agtttgcttt ttgggtgagg gaggtccagc actccggcgg accggtgctt gagcttgctt
     7921 caggtgcggg gcgaattctt acggtgcttg cggaggccgg tgttgacgtc tatgggctgg
     7981 aagcttcttc ggagatgatt gagcttggaa aaagggctat gcacgcgtta cccgtcgaaa
     8041 ccaaacagca catccgggtg gttcaggcgg acatgcgcgt tttcacattc gtgcggaagt
     8101 ttcctctcgt cattattcct ttcacaagtt tttggtataa ctttaaaaat gctgcgattg
     8161 acgatattgg atgggatttt gaaacttctg agaaaagcga aaaagaattt caggaacaca
     8221 ttgagcgtca ggctgaaaac tgtctgtctt caatagtcgg cgcgcttgcg ccgagcggaa
     8281 ggtttataat tgataatccc ttttacacta cgcattggca aaaagagaac ggccgcgata
     8341 aacgggacca ctgcaggcgt tggtggagca ggatggtaga acgattcgga tttgatgttt
     8401 acatacggaa gccctttgat tttccggatg atataattcc tgttcttgag ggaataaaga
     8461 aataaatttc atatataaga ggagcggttc actcgctctt ttttcttgtt gaagttttat
     8521 tccggttgat atagtattca catggctctt ttgttaagca tacggtgcac caaatgaagt
     8581 ggaaacatat tccagcagat ccgagttccc ccagcccgtt tgatcgtgtt gccgattact
     8641 acatagtgga agattatgat atggccgcta tccctaaacc aagccccgaa tttcttcgcg
     8701 aagttgagaa gagcgatgag gaaacaaaag aaatttttag gaaactcggc atcattaaat
     8761 agagaaaaag agccccgatg gccatcgggg ctctttattt tttttccggt tctttgccgg
     8821 agcggttatg gcccttcagt tcgtcaagcg taaagcactc attctgccat agaatcgcct
     8881 gaacgaaccc aagccagcgc atcgcttttt cgcgctcgtc cgcaagtatt aaattttcca
     8941 tctcgtcgag catcgcgcgg caatgcatca tggcggttcg ctttgaatag cacggatggt
     9001 cgtgcggcgc ttccgattta ggaataagtc gtgactcgag caagtgccgg taggccttta
     9061 ccaggaaaag atattgtcct gtggtcatac gcctctccga ttaagcatat gaaggtgcta
     9121 tgtaaagttt atcgaaagga aattgttctg tcaaaacaaa aaagagcgat tcattcgctc
     9181 tttaaaatgt gtctaccact gtttctttgt gagttagccc catgtcgcag tgaactgata
     9241 ttccgtatat gggcgatagt attcgtcgct tcccggtgag tgttcgtgct ctcttgtttc
     9301 gaggcgaagc ttgctttgcg cttttcggta gtcgcccgcg atgtgtcggc atgcctgtct
     9361 ccagccatca agatactccg ggtctccatt gcttgtgaat gaggaaataa ccacgctgtt
     9421 tcttccctcc gcagccgcct ctcgggtttt tggaccgatc gttttgtgaa tgtccggggt
     9481 gatcttttca gtgatcgcat cgactcgtgc ttggcggtct ctcgagtttt tttctttctc
     9541 ggcggtttta ccggcttcaa aactcttagt gaggctaacc agttcgtccg tgtcaacgag
     9601 ttcgggcctt tcgggcgtgt ccattttttc tccgcatttt aaagaatcgt ctcaaaagga
     9661 gtatactcga acccgaacat ttttgcaagg ccttttgacg tgattgtgcc cttatttact
     9721 taccagacct agcctggtat ttttcccatg ccctgcggga tctccagtcc atgacagcgc
     9781 agtacgccgt caataccccg acaaacacca tcatggggag aaagagaagt gaggttataa
     9841 gtacgttcat tttcccgtta gtattataaa tgcaataaga aacttttttt tcgctccagt
     9901 tcttcatccc aatttttata aacagaaaca gtgttaccca actccccaga aaaattccaa
     9961 gagtgaccag caatagtgcg ttaagcaaga catttctcct ttctagagaa gcgtatcgat
    10021 gggagtgtat tcaagcccga acgtttttgc aaggcctttt gacgtgactg tgccttcata
    10081 aattgaaaca gagttccgca gggcgggaca ttcttttagg gcgccgacaa aaccgtgacg
    10141 gatactttca agcatgaatg gcttcaccga ctccgaaaaa atcggcgcgg accgttctcc
    10201 aacgcccccc ggaatgttcg ggatgccgaa atacttaacg ccttcaagct cgacatacgg
    10261 gttctcatgc gaggtggcgc gggaaaattc gcatgctcct ccct
//
LOCUS       DATDSU010000065         5798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1403889, whole genome shotgun sequence.
ACCESSION   DATDSU010000065 DATDSU010000000
VERSION     DATDSU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403889"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1214)
                     /locus_tag="VI981_02220"
     CDS             complement(297..1214)
                     /locus_tag="VI981_02220"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEY4479147.1"
                     /translation="MENISIRMTPKDFFLHLGVVAGLYVSSISLLALLFQIINSAFPD
                     ALESYRDPYSSGIRFAIASLIIIYPSFIFLSWLLNRDYEKTPPKRELGIRKWLVYFTL
                     FVAGIAVVIDLIILINTFLSGEVTVRFILKVIAVLVVAGFIFGYYLSDLKQKTPRSRN
                     KVVAIIVSIIVLASIVSGFVIMGSPGSQRDRRLDEYRVNHLQSIQSEVIYYWQRKEKL
                     PATLGDLEDPVRGFMMPNDPETDAAYEYEILAPESFRLCATFKTDSTATGKVPQPSGP
                     YYGNETWSHGTGRVCFDRPIDKDLFPPRK"
     gene            complement(1224..2411)
                     /locus_tag="VI981_02225"
     CDS             complement(1224..2411)
                     /locus_tag="VI981_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479148.1"
                     /translation="MKEICPECSGKPINHGVAKTTAAMEAFVRFTGKPFMGSFSSFSI
                     FFDKLFPIAFRFCSLIGIGNITHVSDSKDSVRTHCLWESARKRNIMMHQFRLFNQPLN
                     MFIAEHNGERIVFEALPRPKYHSLNSGDMDDKGLIKKIFDEHDIPVALGKAVFSLAEA
                     KRLFGQLRKPVITKPSLGSHGRHTRIHINSQGELERGFSSAKRLSPWVIVEEELKGSV
                     YRVALVGGRVIGVLRRDSPTVVGDGKHTIKELIHETNAHPWRVGNFHKIEITDFVRRE
                     LAYQNLSLDSRPARGRKIMLHPKVGRTQGGTNANVTKHVHPENIKMFERIASALEDSL
                     VGVDFIIEDIAQSWKEQDRSGVIECNSAPFLDLHHFPYAGEPIDAMGELWKIVFPNSK
                     LSV"
     gene            complement(2548..2760)
                     /locus_tag="VI981_02230"
     CDS             complement(2548..2760)
                     /locus_tag="VI981_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479149.1"
                     /translation="MQTGSAIGGGLLFLLAMLVWGQSRNFWVGKLLAVVFFTGACVLG
                     AVTFKNPKVVGNGSEIPGTNILSGVR"
     gene            complement(3293..4414)
                     /gene="ychF"
                     /locus_tag="VI981_02235"
     CDS             complement(3293..4414)
                     /gene="ychF"
                     /locus_tag="VI981_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003214682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY4479150.1"
                     /translation="MSLSIGIVGLPNVGKSTLFNALTLNAVPAENYPFCTIDPSVGVV
                     AVPDHRLWKLSAFSKSAKTIPAIVEFVDIAGLVKGASEGEGLGNKFLQNIRETDAILE
                     VVRIFEDDNIVHVQGKIDPLSDIETINLELALADLDTVKKRLINIGKEIKRGDKAALL
                     EAAVLGKIEKTLEAGKRAASIDFSSEEKSIVKQLHLLSMKPVLYCLNRKVGGKNLDDM
                     KDERYQRLVNSFNETGEKWVRVDAAIEHEIKDLTLEEKESFRKELGSIDGSTGLTVGS
                     GVDALIRKSYELLNLISYFTTGEDETRAWTIGQGWTAPVAGTAIHTDFNDKFVRAEVI
                     EWDTLLDAGSYTKAREKGLVRTEGKEYIVQDGDVVEFKI"
     gene            complement(4452..4658)
                     /locus_tag="VI981_02240"
     CDS             complement(4452..4658)
                     /locus_tag="VI981_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEY4479151.1"
                     /translation="MKKETLQIGGMHCGACATGIQMLVSGMDGVQNIFVDYDGKKADI
                     EYDEGKIKKEDLIKGIAELGYTAQ"
     gene            4791..5072
                     /locus_tag="VI981_02245"
     CDS             4791..5072
                     /locus_tag="VI981_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479152.1"
                     /translation="MKGGDRTVSAPLKFPDIVSTIQRAILGDSGASALRLDSEIQKRY
                     LASVRETLGSELGRVVKLPDGKQWTVATLANYIRVYQGTFEEKAQPKVA"
     gene            complement(5509..>5798)
                     /locus_tag="VI981_02250"
     CDS             complement(5509..>5798)
                     /locus_tag="VI981_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479153.1"
                     /translation="KQNLFWAFIYNIVAIPVAGGIHLLFTQASFGVAAPWLLALTSKL
                     GGFGRVLLALSQATLRPEIAGFAMAFSSISVVLNSLLLKRYVPPIERSTKP"
BASE COUNT         1619 a         1360 c         1256 g         1563 t
ORIGIN      
        1 ttattacaac aacttacgca ttcacacgag tctgggaatg gcgccaaaga aattcacaga
       61 aaaatttgcg caagagaaag aaatcaaata cactacaagt caagagaaac taactgtcta
      121 agaaaagggg tacacttcaa tggagcaagc gatgctgacg tggaaagatc atccggacga
      181 ttgcatcgtc gttcgagacc tctcggatcc ttcggaggct cagcctttcc ctgaaaaagt
      241 ttcttcatcg ccataaccgc cacggcggtt tttttattac ctgatcggtt ttgccattac
      301 tttctcggag ggaaaagatc tttgtcgatg gggcgatcaa aacaaacacg cccagtgccg
      361 tgtgaccatg tttcatttcc ataatatggg ccggatggtt gaggaacttt ccccgtcgca
      421 gtggaatcag tcttgaaagt agcacagagt ctgaaactct ccggcgctag tatttcatat
      481 tcataggccg catcagtctc tggatcattc ggcatcataa agcctctgac cggatcctca
      541 agatcgccta gcgtcgcagg caatttttct tttctctgcc agtaataaat gacttccgat
      601 tgaatacttt ggagatgatt tactcgatat tcatcaagac gtcgatcgcg ctgcgatccc
      661 ggtgaaccca taatgacaaa gccactcaca atagaagcga gaacaatgat cgagacaatt
      721 attgcaacaa ctttattccg agaccgagga gttttttgct tgagatccga caagtaatac
      781 ccgaaaataa atccggcgac aacgagcact gcgatgacct tgagaatgaa tcgcaccgtc
      841 acttcaccgc ttagaaacgt gttgatgaga attataagat cgataacaac agcgatgccg
      901 gcgacaaaga gagtaaaata gacgagccat tttcgaatac cgagttcccg cttcggagga
      961 gttttctcat aatctcggtt caagagccac gacagaaaaa taaatgacgg atagataata
     1021 atgagagaag cgatcgcaaa gcgtatgccg ctagaatacg gatcgcggta cgactcgagt
     1081 gcatcgggaa acgctgaatt aataatttga aaaagaagcg ccagtaaact gatcgaactg
     1141 acataaagcc cagcgacgac gcccaaatga aggaaaaaat ccttaggcgt catgcgaatt
     1201 gatatatttt ccatagtgtt taattatacg cttagttttg aattcggaaa aacgatcttc
     1261 cataactctc ccatggcatc aataggttct cccgcatacg gaaagtggtg aaggtctagg
     1321 aaaggtgcgg aattacattc gatgacgccg gagcggtctt gttctttcca tgattgagca
     1381 atgtcttcaa taataaaatc gactcctaca agcgaatctt cgagcgccga cgcgattcgc
     1441 tcaaacattt ttatattttc cgggtggacg tgcttagtaa cattggcatt ggttccgccc
     1501 tgtgtccttc ccacttttgg atgaagcatg attttccgtc ctcgtgcggg gcgtgaatcg
     1561 agcgataggt tctgatatgc aagctcgcga cgcacaaaat cggttatctc tatcttgtga
     1621 aaattaccga cacgccaggg atgggcgttt gtttcgtgta ttaattcttt tatcgtatgt
     1681 ttcccatctc cgaccaccgt gggcgagtcc cgccgaagca caccaattac tctgccacct
     1741 acaagggcga ctctataaac cgaacctttg agttcctctt caacaattac ccaaggagaa
     1801 agccgtttag cgctactaaa tcctcgctca agttcgcctt gtgagtttat atgaattcgc
     1861 gtgtggcgcc cgtgcgatcc gagcgacggt ttcgttatga caggctttcg taattgacca
     1921 aatagccgct tggcctcggc caaagaaaaa actgccttgc ctagagcgac gggaatatcg
     1981 tgctcatcga aaattttctt aatgagacct ttgtcatcca tgtctccgga attgagagag
     2041 tgatattttg gtctcggcag agcttcaaaa acaattcttt ctccgttatg ttctgcgata
     2101 aacatattga gcggctggtt gaagagccga aattgatgca tcataatatt gcgttttcgt
     2161 gctgattccc atagacaatg ggtacgaaca ctgtctttcg agtcagaaac atgagtaatg
     2221 tttcctattc caatgagaga acaaaagcga aacgcgattg ggaaaagttt atcaaaaaaa
     2281 attgagaagc tcgaaaaact tcccataaac ggcttgccgg taaaacggac aaacgcttcc
     2341 atcgcggcgg tagtcttagc cactccgtgg tttatcggtt taccgctaca ttcaggacag
     2401 atttctttca ttcgaatatt ctatcaaata tgccggcttg ggggcgtacc ctctgtgtga
     2461 aatctacgcc tccgttgcga gatgcaacaa gaacaagaca agaataattc atacaaaaaa
     2521 agcgtgagca atgtgactca acgcttatca tctgacacct gaaagtatgt tggtgccggg
     2581 aatttcactt ccattcccga ctacttttgg atttttgaat gtcacggctc caaggacgca
     2641 cgcgccagtg aaaaagacaa cggcgagcaa ctttccaacc cagaaattac gtgactgtcc
     2701 ccacactaac attgccaaca gaaaaagtag gccacctccg attgctgatc cagtttgcat
     2761 acgtgtaatg agttattagg tgagttaaga acgctccgac gtcaaatcag aatgctacgg
     2821 catataatgc tgaaacgcaa atacacattt agatattaca aactacaacc ataacggaaa
     2881 aattgactcg taattttgag agtgatatga ataccgcgtg taggaatggc cggtagaacc
     2941 atcgaaagcc tagaccctgc tcattttagg acatacctga actaaatcgc atgcacttta
     3001 gacatttggt tgggagactt ggaaacttgt ccgccagata tactagggtc aagcgaacac
     3061 ttggggccga tgctgatcac gaatttactt tcctggtcac cggccccttc ctttttttct
     3121 gaatcttgat ttcagtttta tccctcgacc tactcgggcc gattgacaaa atcaaatctt
     3181 aaattcgact caccctacgt cgctgcgctc ctcaggatct ccgaattcaa aatcagattt
     3241 tgaattcaac ctactccgaa ctggagttcg gagtctcgga tttaaaattt tgctaaattt
     3301 taaactctac gacatcgccg tcttgaacaa tgtattcctt cccttccgtt cgaaccaagc
     3361 ctttttctcg cgccttagtg tatgatccag cgtcaagcaa cgtgtcccat tcaataactt
     3421 cagcgcgcac gaatttatca ttgaaatcag tgtgaattgc cgtgccggct acgggagccg
     3481 tccaaccctg tcctatggtc catgcccgcg tctcgtcttc tcctgtcgta aaataagaaa
     3541 tcaaattgag aagctcgtaa ctttttctta taagcgcatc tacgccactt cctacggtga
     3601 gccctgtcga accgtcaatg gaaccgagtt cttttcggaa tgattctttt tcttcaagcg
     3661 tgagatcttt tatttcatgc tcgattgccg catcgacacg cacccatttt tctccggttt
     3721 cgttaaaact atttacgagc cgctggtagc gttcatcttt catatcgtca agattttttc
     3781 ctccgacctt acgattcaga caatagagaa ccggcttcat ggagagaaga tgcaattgtt
     3841 taacgatcga tttttcttcc gaagaaaaat cgatcgaagc cgcgcgcttc cctgcttcaa
     3901 gcgtcttttc aatttttcca agtaccgcgg cttcgagaag tgcggcttta tcgccgcgtt
     3961 tgatttcttt accaatattg atcagacgtt tcttaacagt atcgagatcc gcgagcgcca
     4021 attctaaatt aatagtctca atgtcgctta acggatcaat cttgccctgc acgtgaacga
     4081 tattgtcatc ttcaaagatc cgcaccactt caagaatggc gtcggtttcc cgaatatttt
     4141 gcagaaattt atttccaagc ccttccccct ctgaagcgcc cttcacaagt ccggcaatgt
     4201 ctacaaattc aacgatggct ggaatggttt tagcagattt tgaaaaagcg gaaagtttcc
     4261 ataatcggtg atcaggcact gccacaaccc cgacggaagg atcaattgta cagaacgggt
     4321 agttttccgc cggcacggca tttagcgtta atgcattaaa aagcgttgat ttacctacat
     4381 tgggtaggcc cactatacca atacttaaag acatgcggcg aagtataacc tacaaattgc
     4441 agagtctcaa tttattgggc tgtataacct agctctgcta tgccttttat aaggtcttct
     4501 ttttttattt ttccctcgtc gtattcaata tctgcctttt taccatcata atctacaaaa
     4561 atattttgta cgccatccat tccagacacg agcatttgga ttcctgtggc gcaggctccg
     4621 cagtgcattc ccccgatttg tagtgtttcc tttttcacga ggattaatta tagctaaatt
     4681 ctgctcgaca cggtgtgtaa aactacaata atttgactcg aagcgagaga gtggcataat
     4741 atcaagagac tcctcgattc cgggtgagtc ggcaaactgc cctgtggcaa atgaaaggtg
     4801 gtgatcgaac cgtgagcgcc ccattgaagt tccccgatat tgtctcgaca attcagcgag
     4861 caatcttggg cgatagtgga gccagtgcac tgagactcga cagcgaaatc cagaaaagat
     4921 acctcgcaag cgtcagggag acgcttggta gcgagcttgg ccgtgtcgtc aagttgccgg
     4981 atggcaaaca atggactgtg gcaactcttg ccaactacat ccgtgtttac caaggaacat
     5041 tcgaggaaaa agcacaaccc aaagtcgcat gaaatgcaca accccgaagc cggtgaaagg
     5101 attcaccggc tttgttgtgt ctattaaaat ttgtgatatt cttttaatag attatgaaga
     5161 aagaactcag atcagtattg tggctgatag ctgtatgccc gtggtttgtt ccgacgttgg
     5221 tgacaatcgc tacgattagc gcattttttt cggtcgccgt cagacacgta aatctgggca
     5281 gacagatccc tacccacttc tggtggatag ctggtctaat ttatgtattg ctcatcgctc
     5341 ttgcgattgt agccgaacta aaatggctgc gggaaatccg aaagcaaccc atgcctcgac
     5401 agccgacata gagtcggttt ttttgtttag taaacctgta tacaaactgt tgatgattcg
     5461 tgcaatttct aacctgcaat acttctcaag gtttaacctt gttctaaatt atggctttgt
     5521 agagcgttct ataggaggaa catagcgttt caacaatagc gagttaagga ccaccgaaat
     5581 cgaggagaag gccatggcaa agccggcgat ttccggccga agcgtagctt gcgaaagcgc
     5641 gagcagtaca cggccaaatc ctcctaattt tgatgttaaa gcgagaagcc acggagcggc
     5701 gacaccaaac gaagcctgtg taaaaagtaa atgaatgcct ccggcgacag gaatagccac
     5761 aatattgtat atgaatgccc agaagagatt ctgcttta
//
LOCUS       DATDSU010000066         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1889320, whole genome shotgun sequence.
ACCESSION   DATDSU010000066 DATDSU010000000
VERSION     DATDSU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889320"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VI981_02255"
     CDS             complement(<1..296)
                     /locus_tag="VI981_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEY4479154.1"
                     /translation="MKQHQQTLDKWFKEKGWEYWSPLSILARLMEETGEFARLVNHLY
                     GDKPKRDDEKEQNLEEELGDILYTLMCFANSKGIDLDKTIQKTFDKVMSSDKDR"
     gene            complement(310..765)
                     /locus_tag="VI981_02260"
     CDS             complement(310..765)
                     /locus_tag="VI981_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY4479155.1"
                     /translation="MEKPLKFIKSQKLMVIASRDEKDVWVANVYFGVDEKATIYFISQ
                     KNTKHSQMILKNPNIAFSIAWFDPGNHKNRKAIQGLGVCRQAKNPTEVANGIKLLYKK
                     FPDLRDILTVKWIMTNVWGSKIWVLKPTYMKYWDDEIYGDDETEEFTLK"
     gene            complement(894..967)
                     /locus_tag="VI981_02265"
     tRNA            complement(894..967)
                     /locus_tag="VI981_02265"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(933..935),aa:Ala,seq:cgc)
     gene            complement(1007..1342)
                     /locus_tag="VI981_02270"
     CDS             complement(1007..1342)
                     /locus_tag="VI981_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY4479156.1"
                     /translation="MKKEQNFYSKVYDVVKKIPAGKVATYQQVATIISTPRAAQAVGW
                     ALRALPDYNNIPWQRVINSQGMISIENLRAPKSLQVKLLREEGVEVKEVDGNYFVDLN
                     TYIWRPYRK"
     gene            complement(1350..1420)
                     /locus_tag="VI981_02275"
     tRNA            complement(1350..1420)
                     /locus_tag="VI981_02275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1386..1388),aa:Pro,seq:cgg)
     gene            complement(1537..>2674)
                     /locus_tag="VI981_02280"
     CDS             complement(1537..>2674)
                     /locus_tag="VI981_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4479157.1"
                     /translation="RDFTMNAIALEFPISNSQFPKLIDPFDGQADLKKKMIRAVGNPT
                     ERFDEDALRMMRAVRLATELDFEIEKNTFMAVKKLSGHLAFIAKERIRDELIKIIAAV
                     KARDGIELLQDAGLLRHIMPELEEGIGVTNRPKIFTIWEHNLKALEYGAKTRFSPEVC
                     LIALMHDIGKQRAKGPMKNDEWTFYGHDMIGSKMAAEILTRLHFSSDFVKKAAILIRW
                     HLFKYDPDEGVTDSSVRRLIRNVGAENMADLVKIRICDRMGMGVPKAVPYRLRHFQFR
                     VEKILREEEAPTPKMLKIRGGDIMRILGIEPGPKIGQIIEVLVQEVIDEPEKNNSEYL
                     VERVKELGGLSSEELKKIASEAEEKVELAEDERISKIKSKYWVK"
BASE COUNT          687 a          654 c          526 g          807 t
ORIGIN      
        1 tagcggtctt tatcactcga catcacctta tcgaacgttt tttgaatcgt tttgtcgaga
       61 tcaataccct tggaattcgc aaaacacatg agagtataga gaatatcgcc aagttcttct
      121 tcgagatttt gttctttttc gtcatcccgc ttcggcttat cgccgtataa atggtttacg
      181 agccgcgcaa attctccggt ttcttccata agccgcgcca aaatagaaag cggagaccag
      241 tattcccacc ctttttcttt aaaccactta tcgagggttt gttgatgttg cttcatggaa
      301 attcgtacgt tatttgagag taaattcttc ggtttcatca tcgccgtaaa tttcatcatc
      361 ccaatatttc atgtaggttg gcttgagaac ccagattttg gaaccccaga catttgtcat
      421 tatccactta acggtaagta tatcgcgaag atcaggaaat ttcttataca acaatttaat
      481 tccgtttgct acttcggttg ggttttttgc ttggcggcaa accccaagtc cttgtatcgc
      541 tttcctgttt ttgtgattac cggggtcaaa ccaagcaatt gaaaaagcga tattcggatt
      601 tttcaatatc atttggctat gttttgtgtt cttttgactg ataaaataaa ttgtcgcctt
      661 ttcatcaacg ccaaaataaa cattcgcaac ccaaacatcc ttctcatcgc gagaggcaat
      721 caccatcaat ttttgacttt tgataaattt tagtggcttt tccataattt tacctaaaac
      781 cataggccac aattcgatac aacttagtat gtgggcctat agggagaatg gttcgacttc
      841 gctcaccaca agtcgaaccc ctgcctccac aacctatccc gacactatag cattggtgga
      901 cctagggaga atcgaactcc cgtctcagca atgcgaatgc cgtgtattac cactatacta
      961 taggcccgaa ggtctacagt gtcgggataa aatgcggacg aggcacttac tttctatacg
     1021 gacgccaaat gtatgtattc aggtcaacaa aataatttcc atcaacttct ttcacctcaa
     1081 cgccttcttc gcgaagaagc tttacctgaa gcgattttgg cgcgcggagg ttttcaatcg
     1141 aaatcattcc ctgtgaattg ataaccctct gccaagggat attgttgtaa tccggaagcg
     1201 ctcgaagcgc ccagccaact gcttgggccg cgcgcggggt tgaaataatc gtcgcaacct
     1261 gctgatacgt cgcgactttt ccggccggaa ttttttttac cacgtcgtat actttgctat
     1321 aaaagttctg ttcttttttc ataaccaact cgggcctccg ggaatcgaac ccggtctaca
     1381 cgaacccgaa tcgtgtgtac taccggtata ctaaggcccg ataagtcctt ccttttatat
     1441 cgcgaataaa gaaaaaaatc aatcataact ccaatgttaa cattctccat acgatcgtat
     1501 gagaaatgtt aacattgact tttgcgaaaa aagaacctat ttcacccaat actttgattt
     1561 tatttttgaa atacgctcgt cttctgcaag ttcaaccttt tcctcggctt cagaagcgat
     1621 ttttttgagt tcctccgatg aaagccctcc gagctctttc acacgctcta caagatactc
     1681 ggaattattt ttttccggtt catcaatcac ttcctgcacg agaacctcga tgatctgccc
     1741 gattttgggc ccgggctcaa ttcccaaaat tcgcataatg tctccaccgc ggattttgag
     1801 cattttcggc gtgggcgctt cttcttcgcg caagattttc tcaacccgaa actggaaatg
     1861 acggagtcga taggggactg ctttgggaac ccccatgccc atccggtcgc aaattcgaat
     1921 tttcaccaag tccgccatgt tttctgcgcc gacgtttctg ataagccgcc tgaccgaaga
     1981 atcggtaact ccttcgtcag gatcatactt gaatagatgc caacgaatca aaatcgccgc
     2041 cttttttaca aaatcagaag aaaaatgaag gcgcgtcaaa atctcggccg ccatttttga
     2101 tccgatcatg tcgtgcccgt aaaacgtcca ttcgtcattt ttcatcgggc ctttggcgcg
     2161 ctgcttcccg atgtcgtgca tgagcgcgat tagacaaact tccggagaaa atctcgtctt
     2221 tgccccgtat tcgagggctt tcagattatg ttcccaaatc gtaaaaatct tcgggcgatt
     2281 cgtaacacca atcccttctt caagctcggg catgatatgt cgaagaagcc cggcatcttg
     2341 cagaagctcg attccgtcac gcgcttttac ggcggcaatt atttttatga gttcatcacg
     2401 aatgcgttct tttgcgataa aagctaaatg tcctgaaagt tttttgaccg ccataaaggt
     2461 atttttttcg atctcgaaat cgagttctgt cgcaagacga accgccctca tcatacgaag
     2521 agcatcttcg tcaaatcgtt cagtcggatt cccgaccgca cgaatcattt ttttcttcaa
     2581 gtcagcttgt ccatcgaatg gatcgattag ttttgggaat tgagaattgg aaattggaaa
     2641 ttccagcgcg attgcgttca tggtgaaatc gcgt
//
LOCUS       DATDSU010000067         7646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1068560, whole genome shotgun sequence.
ACCESSION   DATDSU010000067 DATDSU010000000
VERSION     DATDSU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068560"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VI981_02285"
     CDS             <1..484
                     /locus_tag="VI981_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479158.1"
                     /translation="TDVSLRPKGVTCELAVRYTIKKDWLLVNSVDTMTLGVPHTISPD
                     NLPLESDPTEAGRLGISTVNLHDTLFFKSENEALNFFVNTYNLNGTGAKIQNQGFQNF
                     WNKESAEKSGYPFAGEGGEAFIIVSGTSNSSQNKCYIGELSLVSKETTYHDKPCVIIN
                     "
     gene            complement(571..759)
                     /locus_tag="VI981_02290"
     CDS             complement(571..759)
                     /locus_tag="VI981_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479159.1"
                     /translation="MKTKQSNTKTVVPFSQLLDVDLRRDFLRIRAGGSRKCLSTPPPM
                     SGIEARMPRRRENVLETL"
     gene            859..1632
                     /locus_tag="VI981_02295"
     CDS             859..1632
                     /locus_tag="VI981_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY4479160.1"
                     /translation="MENGDITSDVTTEPKVLTLKERREAKRQEKLARKASVENKGGGS
                     RFFLWLFVVIVLAAVVVTMARLAKNKGETDTVATTILESVTAKDWMRDNLSASTTLLE
                     YSDFECPACRAYYPVLKQIHDEFGSKIRFVYRHFPLTQHANARLAARAAEAAGVQGKF
                     WEMHDRIFDTQETWAAKPDAEQKFIALAEELGLDMTKFEKDMRSKSIGDKIDSHYSGG
                     QSYGVNVTPTFFINNTRFTFNSFEEMKKAIQDGIDGNVK"
     gene            1616..2062
                     /locus_tag="VI981_02300"
     CDS             1616..2062
                     /locus_tag="VI981_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY4479161.1"
                     /translation="METLNNRALYLKRLAILAIVIAFVGFVDATFLTAEHYLDGPIPC
                     SITEGCEIVTTSKFSVLFGLPIALYGALFYVSIIVLSILYLDKKNERMLKLAAYFASI
                     AFVVSLGLVGIQAFVLHAYCFYCLMSAFLSTLLFVNGRFMLKFSLK"
     gene            2148..2597
                     /locus_tag="VI981_02305"
     CDS             2148..2597
                     /locus_tag="VI981_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY4479162.1"
                     /translation="MILVYQSLIIALALFGFGISFHIFRTKKRADVLVCPLNLDCTTV
                     VHSEYSRLLFVPLEWWGMSYYLIAAAMYTLFITTPESYTGPAIIFILTISTLAFLVSI
                     YLTLVQLFILKHFCTWCLISALISITIFIIGFSISEYSIISLKSFFI"
     gene            2594..3070
                     /locus_tag="VI981_02310"
     CDS             2594..3070
                     /locus_tag="VI981_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479163.1"
                     /translation="MSKPALSWKAPEYHHFEKSNDWYWALWIIAIALSIAAFIFGNLL
                     FGLLVILCAVTLHLHANKPPRILSIELTERGVKVDTLLYPYETLDSFWVSVPHHEHGH
                     HFSHILIKSQKTLMPLIVVPIKEQNPETVREILQKHLVEEEHHEPLGHKILEYLGF"
     gene            3253..3516
                     /locus_tag="VI981_02315"
     CDS             3253..3516
                     /locus_tag="VI981_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479164.1"
                     /translation="MKAYVYILKNTGKTFYIGSTVNLERRLRAHKSGNTQTTKNMKNV
                     ELVLAQEYESLMIARKIERKLKNMKRKDYLEKIVDDGYIRMQV"
     gene            3522..3593
                     /locus_tag="VI981_02320"
     tRNA            3522..3593
                     /locus_tag="VI981_02320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3555..3557,aa:Arg,seq:gcg)
     gene            3778..4236
                     /locus_tag="VI981_02325"
     CDS             3778..4236
                     /locus_tag="VI981_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4479165.1"
                     /translation="MIKHSAGVLVYRKHNSVIEYFLVHPGGPYWSNKDKGIWGIPKGE
                     IHDGEGMFETAQREFTEETGFTVEGNFTPLGSVKKKDGKIIHGWAIEADLDASKLKSN
                     TCTIEWPPRSGKHIEIPEIDRGEYFNFERARNKIHPAQIPLLEKLSELLN"
     gene            complement(4271..4543)
                     /gene="rpsT"
                     /locus_tag="VI981_02330"
     CDS             complement(4271..4543)
                     /gene="rpsT"
                     /locus_tag="VI981_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY4479166.1"
                     /translation="MAITSSAKKALRASARKREFNLARKAAIYDITKKIKRLIKENKA
                     TEAKTLLPQAYKTLDKAAKTNFLKKNTASRKKSRLAQMIKRASVTK"
     gene            4692..5261
                     /gene="ruvA"
                     /locus_tag="VI981_02335"
     CDS             4692..5261
                     /gene="ruvA"
                     /locus_tag="VI981_02335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY4479167.1"
                     /translation="MIATLEGKITHKDLKSVICNVNGVGYKVFVTLDTLEKLKKDDSV
                     FLFTYLAVRENALDLYGFTTKEGQDFFELLLTVSGIGPKSAISILSIASPETLRKAVS
                     SGDNSYLTKVSGIGKKTADKIILELREKIGSLESEGGGNIQGEVDAVEALKSLGYSQN
                     EARDALKKVAKEIVSTSDKVRAALKLLGK"
     gene            5298..6692
                     /locus_tag="VI981_02340"
     CDS             5298..6692
                     /locus_tag="VI981_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY4479168.1"
                     /translation="MESILRLGRKIIPRKIFEALQPIYHRKLAFLAAITCGFPSRKLN
                     VVAITGTKGKSSTVEFVNSILETAGYKTAVLGTIRFKIGEKSQPNLHKMTVPGRFFVQ
                     KFLRNAVNSGCEYAILEMTSEAIKQHRHKFIDLDALIFLNLAPEHIESHGSYEKYVQA
                     KLALAELLTKSRKKRKLIVVNSDDKEAPRFLSIPIKEKYPFSIRVAEPFTLSSNESEF
                     YFHEERITLHVPGQFNIYNALAAATFALTQNISLQNIREGLERIHEIPGRVQKIKVSP
                     DQDFTVIVDYAHTTDSLEKLYQTFSPLLGDLVSKSKKWRYEAINKEATHLETKSPSPS
                     PSPLPGKKRLICVLGNTGGGRDKWKRVEMAKIADTYCDSIILTNEDPYDDNPREIVED
                     MRRGIKTHTAKIIMDRRDAIRDAIGMAEKGDTVLITGKGTDPYIMGPIGSKIPWSDQK
                     VVKEELARLFETRS"
     gene            6710..7627
                     /gene="mutM"
                     /locus_tag="VI981_02345"
     CDS             6710..7627
                     /gene="mutM"
                     /locus_tag="VI981_02345"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY4479169.1"
                     /translation="MPELPEVQTTVNGINKKLKGLTIRDVWTDYGSPFHTGKKNIKDS
                     RYFLHFKKAVGGATISHATRRAKNILIHLLNNKTILIHMKMTGHLLYGAYSFNKSANA
                     WVASGEGPLKDPRNKFIHLVFTLSNGKYLAFSDMRKFGKVFVEDSEKIETHRDLAQIG
                     PEPLTKEFTLQIFKDQIKKKPNRKIKQVLMDQSVVAGVGNIYSDELLWLSSVHPASTV
                     KKIPSSHMKKIYLSLIEVLKKGIDFGGDSMSDYRNLEGERGRFQHTHNAYRLTGQRCK
                     RDGCGGIITRIKIGGRSGHFCNRHQRFFK"
BASE COUNT         2305 a         1667 c         1637 g         2037 t
ORIGIN      
        1 tactgatgtg tctctacgac ctaaaggtgt tacttgtgaa ttggcggtaa gatatacaat
       61 caaaaaggat tggctgttgg taaatagtgt agatactatg actcttggtg tcccacatac
      121 catttcaccc gacaatctac ctcttgaaag tgatcctaca gaagctgggc gactaggaat
      181 atcgacagta aatcttcacg atacattgtt tttcaagagc gaaaacgaag ccctgaactt
      241 ttttgtaaat acatataatc tgaatggtac cggtgccaag attcaaaatc aaggtttcca
      301 aaatttctgg aataaagaaa gtgcagaaaa atctggttat ccattcgctg gtgaaggagg
      361 cgaggcattt ataatcgtat ccggcactag taactccagt cagaataaat gttacatcgg
      421 ggagttatca ctcgtaagca aggagacaac atatcacgac aaaccgtgcg tgattattaa
      481 ctaaaagcga ttacagaatt tgccgcctca gccagaggct gatcagcctt gggctgaaaa
      541 aaacgccact taccgagcgg cgttactctc ttagagcgtc tccagaacat tttccctacg
      601 acgaggcatg cgagcctcga tgcctgacat cgggggcggt gtggaaaggc actttctcga
      661 tccacctgcg cgaatgcgga gaaaatcgcg ccgcaaatca acatcaagca attgggagaa
      721 aggtacgact gttttagtgt tcgattgttt tgttttcata gccaacgtgt acaaccccca
      781 aaagttgcta tacgtttcga cacaaaaatt tgtcattgaa accgtatctg gtaaaatcaa
      841 atctataata taaaacatat ggaaaacgga gatattacga gtgatgtgac gacagagcca
      901 aaagtattga ccctaaaaga acgccgagaa gctaagaggc aagagaagct cgcgcggaag
      961 gcatcagttg aaaacaaagg gggtggtagt cgatttttct tgtggctttt tgtagtcatt
     1021 gtccttgctg cagttgtagt aaccatggcg cgacttgcaa aaaataaagg agaaaccgat
     1081 acggtcgcaa caacaattct cgagtcggtt accgcaaaag actggatgag agacaaccta
     1141 tctgcctcta ccacgcttct cgagtatagc gattttgagt gtcctgcctg tcgggcatac
     1201 tatcccgtct tgaaacaaat tcacgacgaa ttcggatcaa aaattcgttt tgtgtaccgc
     1261 cattttccac tcacacaaca cgcgaatgca cgtcttgccg cgcgtgcagc cgaagcggcc
     1321 ggagttcagg gaaaattctg ggaaatgcat gacagaattt tcgataccca agaaacatgg
     1381 gctgcgaaac ctgacgctga acaaaaattc atcgcacttg cagaagagct tggcctcgac
     1441 atgacgaaat ttgaaaaaga catgagatcc aaatcgatcg gagacaaaat cgactcacac
     1501 tatagcggcg gtcaaagtta tggagtaaat gtcacgccaa cttttttcat aaataacaca
     1561 cgctttacct tcaactcctt tgaggaaatg aaaaaagcca ttcaagatgg aattgatgga
     1621 aacgttaaat aacagggccc tctacctcaa acgccttgcc atactggcca tcgttatcgc
     1681 ctttgtcggt tttgttgacg caacttttct cacagccgag cactatttgg acggtcccat
     1741 cccctgttca attacggaag ggtgcgagat cgtgacgaca agtaaatttt cagttctctt
     1801 cggcctgccg atagccctct atggcgccct tttttatgta agtatcatcg ttctttcaat
     1861 tctttacctc gataaaaaga acgaacgtat gctcaaatta gcggcctact ttgcaagtat
     1921 cgcctttgtc gtttcgctcg gcctcgtggg cattcaagcc ttcgtgcttc atgcatattg
     1981 tttctactgc ctcatgtcgg cttttctttc gacactcctt ttcgtcaacg gacgcttcat
     2041 gttaaaattt agcctgaagt aaactctaat ttcctttctc cgaagcgtgc aagaaaagta
     2101 tagatctact tcagggttta cgtaccctag tctcaaactt taaaaccatg atcctagtct
     2161 atcaaagtct cataatagcc ctcgcacttt ttggttttgg aatttctttt catattttcc
     2221 gcacaaaaaa aagagccgac gtgctcgtgt gcccgcttaa tctggactgc actactgttg
     2281 ttcatagcga atattcaagg ctcttatttg ttccccttga atggtggggt atgtcgtatt
     2341 accttatcgc cgccgccatg tatacattgt ttattacaac tcccgaaagt tatacggggc
     2401 cggcaattat ttttatcctt accatttcaa cgttagcttt tcttgtctcg atttatctta
     2461 cactcgtcca actttttatt ttgaagcatt tctgcacatg gtgcctcata tccgcgctca
     2521 tcagcatcac tatttttatt attggatttt cgatttcgga gtacagtatc attagtctaa
     2581 aatcattttt tatatgagca aaccagcact ttcctggaaa gcgcccgaat accatcattt
     2641 tgaaaaaagt aatgattggt attgggctct atggattatt gccatcgcat tatctattgc
     2701 agcttttatc ttcggcaatc ttctctttgg attattggtt atactctgcg cagtcaccct
     2761 acacctacac gcaaacaagc cgccccgaat tctttccatt gagctgacag aacgcggagt
     2821 aaaagtggac accctactgt atccatatga gacgctcgat tcattttggg tatctgtgcc
     2881 acaccatgag cacggacatc acttttcaca catattaatc aaatctcaaa agacattgat
     2941 gccgcttatt gtcgtgccga tcaaggaaca aaatcctgag acggtacgtg agattctaca
     3001 aaaacatctc gtagaagaag aacaccacga accacttggt cacaaaattc tggaatattt
     3061 gggtttttga cgtccccacc aaggtcgaac cttgaatcga taaaaaggtt cgactttggt
     3121 attttcaact catctcgctt gtgataagtt tgttttgact ttagtattgg aactcaacgc
     3181 tttcgtcgtt caactccgac gcttgcaaag caaggtcgga gccccgacca aagcgtcggg
     3241 gcggataaaa aaatgaaggc ctatgtttac atattaaaaa atactggaaa aacattttat
     3301 atagggagca cagttaatct tgaaagacgc ctaagggcgc ataaatcggg caacacgcaa
     3361 acaacgaaaa atatgaaaaa tgttgaactg gtacttgcgc aggaatatga atcacttatg
     3421 attgcaagaa agatcgaacg aaaattgaag aatatgaagc gaaaagacta tttagaaaaa
     3481 atagtcgatg acggatatat tagaatgcaa gtttaacact cgctcccgtc gttcaacgga
     3541 taggacactc ccctgcgaag ggagaaattt aggttcaatt cctaacgggg gcacacgacg
     3601 aaataaatcc cgacttttct ggattttttc gtacgtgtgc cagacggagc gttgtgagca
     3661 cagcagtgcg aaccgcgatt cggggtcgag aaattttgcg agcgacggtg agcaaaatat
     3721 tcgtgaccac aaacttatca caaataattt cttgagatac atttttttag ccaaaacatg
     3781 atcaaacaca gcgcaggggt tcttgtttat cgcaaacata atagtgtcat cgagtacttt
     3841 ttggttcatc caggtggccc gtattggagc aataaagata aaggaatttg gggtattcca
     3901 aagggtgaaa tacacgatgg agagggtatg tttgaaactg cacagcgtga atttactgaa
     3961 gaaaccggct ttactgtaga aggaaatttt acgcctttgg gatcggtcaa aaagaaagac
     4021 ggaaaaatta tccacggatg ggcgatcgaa gctgatcttg acgcttcaaa attgaagagc
     4081 aacacctgca cgattgagtg gccgccgcga tcaggcaaac acatcgaaat tccagaaata
     4141 gatcgaggtg aatactttaa ctttgaaaga gcacgaaata aaattcatcc ggcccaaatt
     4201 ccgcttcttg agaaattgag cgaattgcta aactaaaaac actccgatag ctgggtgttt
     4261 tcagttttat ttattttgtg acgctcgctc gctttatcat ctgcgcaagc cgcgacttct
     4321 tccgcgatgc ggtgtttttt ttcaaaaagt tcgtttttgc ggccttatcg agcgtcttgt
     4381 atgcctgtgg cagaagtgtt tttgcttccg ttgctttgtt ttctttaata agccgcttga
     4441 ttttttttgt aatatcataa atcgccgctt ttcgcgcaag gttaaattcg cgttttcgcg
     4501 cggatgcccg gagggctttt tttgctgaag atgtgattgc catatgaaga agcgtatagg
     4561 gtttagcgta tagcttgtag ggtgaaatct aacagaaccg ttctaaaaac gcaatagttt
     4621 acgccaactt ctctataccc cggtaatagg accttttgct tcaccacgga gcaagcttta
     4681 tacttagcct tatgattgcg acgcttgaag gaaaaatcac gcacaaagac ctgaaatcgg
     4741 tcatctgcaa cgtaaatggt gtgggataca aagtattcgt caccttggat acgctggaaa
     4801 aattaaaaaa ggacgattcg gtttttcttt ttacatatct tgctgtccgc gagaatgctc
     4861 tcgatcttta cggcttcacc acaaaagagg ggcaggactt ttttgagctt ttgctgactg
     4921 tctccggcat tggccccaaa tctgcaattt caattctttc aatcgcctcg ccagaaactt
     4981 tgagaaaagc cgtttctagc ggcgacaact cgtatctcac aaaagtttcg ggtattggga
     5041 aaaaaaccgc agacaaaatt atcctcgaac ttcgcgaaaa aattgggtca cttgaatctg
     5101 aaggcggagg aaatatacag ggcgaagttg acgcagtcga agcgctaaaa tctctcggct
     5161 attcacaaaa tgaagctcgc gatgcactga aaaaagttgc aaaggagatt gtttctacaa
     5221 gtgataaggt cagggcggct ctcaaactac tgggcaaata aatggacgat ctcgtacgtt
     5281 tgagtatgat gtagagcatg gaatctatat tgcgtctcgg tagaaaaatt attccccgaa
     5341 aaatctttga agcactgcag ccgatttatc accggaagct cgcctttcta gcggcgatca
     5401 cctgcggttt cccctctcgc aagctgaatg ttgtcgccat caccggaacc aaaggtaaaa
     5461 gctcaacggt tgagtttgta aactcaattc tcgaaacagc cggatataaa acggcagtac
     5521 ttgggacaat tcgattcaaa atcggtgaaa agtcacaacc taacctgcat aaaatgactg
     5581 tgcctggccg ttttttcgtt caaaaatttc tccgtaatgc agtgaactcc gggtgcgaat
     5641 acgccattct tgaaatgacg agcgaggcca taaaacaaca tcgccacaaa ttcatcgacc
     5701 ttgatgcact catttttctc aacctcgctc ccgaacatat agaatcccat ggttcgtacg
     5761 aaaaatacgt tcaagcgaag cttgcgcttg cggagttgct cacaaaatca agaaaaaaga
     5821 gaaaacttat tgtcgttaat tcagacgaca aagaagcacc gcgatttctc tccattccta
     5881 tcaaggagaa gtaccctttc tcgatccgcg tggcagaacc gttcacgtta tcttccaatg
     5941 agtctgaatt ttatttccac gaagagcgga ttacacttca cgtgccgggc caatttaata
     6001 tttataatgc cctggcagcg gccacatttg cactcacaca gaatatttca cttcaaaaca
     6061 tacgagaggg gctcgaacgc atccacgaaa tccccggacg agtgcaaaag atcaaagtga
     6121 gccctgatca agatttcacc gtcattgttg actacgcaca cacaacagat tccctcgaaa
     6181 aactctacca aaccttctcc cccctacttg gagacttagt ctccaagtct aaaaaatggc
     6241 gatatgaggc tataaataag gaggccaccc acttggagac taagtctcca agtccgtctc
     6301 caagtccact ccctggcaaa aagaggctga tatgcgttct tggtaacact ggtggtgggc
     6361 gggacaaatg gaaacgagtg gaaatggcga aaatagcgga cacgtattgc gattccatta
     6421 ttctgacaaa tgaggacccg tatgatgata atcctcggga aattgtggaa gatatgaggc
     6481 gcggcattaa aacacatacg gcaaaaatta tcatggatcg gagagacgcg attcgcgacg
     6541 caattggaat ggcggaaaaa ggtgacaccg tgctcatcac tggaaaaggc acagatccct
     6601 atatcatggg gccaattggc agcaagattc cttggagcga tcaaaaagtc gttaaggaag
     6661 aattggcacg actctttgaa acaagaagtt aaaatattga tttacattta tgcccgaatt
     6721 acctgaagtg cagacgactg taaatgggat taacaaaaaa ttaaaaggcc tcacgatcag
     6781 agatgtttgg acagactacg gaagtccatt tcacaccggc aagaaaaata ttaaagattc
     6841 gcgatatttt ctgcacttta aaaaagcagt gggcggcgcg acaatcagcc acgccacacg
     6901 acgagcaaaa aatatactta tacatctctt aaataacaaa acaatcctca ttcacatgaa
     6961 gatgacgggt caccttttgt atggcgcata ttcgttcaat aaatcagcaa acgcctgggt
     7021 ggcaagcggc gaagggccgc ttaaggatcc ccgcaataag tttattcatc ttgtttttac
     7081 gctttcgaat ggcaaatatc tggctttttc tgacatgcgg aagtttggaa aagtgtttgt
     7141 cgaagattca gaaaagattg agacacatcg tgatcttgcc cagattggac cggagccact
     7201 caccaaagag tttacacttc agattttcaa ggatcaaatc aaaaagaaac cgaacagaaa
     7261 aataaaacag gtactaatgg atcaatccgt cgttgccgga gttggcaata tttattcaga
     7321 tgaactactt tggctctcat cggtccaccc ggcgtcaact gtcaaaaaga ttccgtcaag
     7381 tcatatgaaa aaaatatatt tgtcacttat cgaagtgctg aaaaaaggaa tagatttcgg
     7441 aggtgattca atgtcagatt atcgaaattt agagggtgaa cgcggacgat tccagcacac
     7501 acataatgcc taccgattaa caggccaaag atgtaagagg gatggatgcg gcggcatcat
     7561 cacaagaata aaaatcggtg gccgaagcgg acacttctgt aatagacatc aaagattttt
     7621 taagtaagag acgattcggt ttaatc
//
LOCUS       DATDSU010000068         6704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_730102, whole genome shotgun sequence.
ACCESSION   DATDSU010000068 DATDSU010000000
VERSION     DATDSU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730102"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..2231)
                     /locus_tag="VI981_02350"
     CDS             complement(225..2231)
                     /locus_tag="VI981_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479170.1"
                     /translation="MTKKIVGSLVIVIIVIALLTRIGGNRGQQIVESDLVVKENYTPS
                     QEIIFKNNAKLTVKGDLSLKKDSKLTCDGGPLVITVLGNAEINGTLSCILAEDTPSET
                     SGIILIVGKGVTFGSDAVISSNSHIQVVRDFDLALDTQAEIDAAYEDAGKNTGAGPRI
                     GPFVEANATVDVSGSASVSTTPKKDSIVRTLIAQTAYAQTPRDKDGNALDNIVISGNW
                     YVGDGGNPPAGLDIPKPPKGVHKIILYFAFGAEGNVELRDFHLVGPAGRDGDSDENKN
                     CVARGARGENAFRMKVDANNITINNFRLELGNGGHGGTAETKIDCDPGIATGGDGGEA
                     GNFKMNARGSIEIASFHIVPGVGGTGGDATAHGKDGVAGCPGVKGGDATATGGNGGKN
                     KKELAAIGAVSGIANVTVDEVKGGWGGNAAAQPGKGGDGNACKCGGGKGGNGTATGGK
                     GGDASVTIPGAQADANGGNGGNTDSQGATGGVGGSCQGVPQGGTGGAGGDASSKEGPA
                     GKGTSTDGDDGKITNETGGNGGNGGDGCGPGNGGKGGKGKPAGKNGEKGKINCVDVKT
                     DTKVQVGTPPPSATPPPVTTPPPTQKAKEKVINYNGKYLPIDQLIIEDEVGCGADHYH
                     AARGSVVATDGSTVYDPGPQCGYGKVKDKPTLEVEVTAQIKVGL"
     gene            2328..2888
                     /gene="msrA"
                     /locus_tag="VI981_02355"
     CDS             2328..2888
                     /gene="msrA"
                     /locus_tag="VI981_02355"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY4479171.1"
                     /translation="MAVFGGGCFWCTEAVFSAGGGSAYGGKIFKGIISVEPGYAGGLE
                     DTATYKQVFGGTTGHVEVIRIEFDPSRISFRELLTVFFGSHDPTTLNRQGGDVGAQYR
                     SVIFYTTPAQKNESDKFIKELNDSNEMGEPIVTAVEPLVEFIPAEDYHKDYYARNQEQ
                     GYCQIVINPKLEKVQQKFEALIKKSI"
     gene            3098..3703
                     /locus_tag="VI981_02360"
     CDS             3098..3703
                     /locus_tag="VI981_02360"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY4479172.1"
                     /translation="MMNIVLATNNPSKAEHIKSQFAKLPIELRTLQECGITEEPVENG
                     KTLEENALIKAKFAWERTGKCSIADDTGIFIDALGGAPGIHSGRFAGVGKTIEEGQAF
                     VIEKMKDVKEQDRSAVFRTVAVVFEADGTHSVFRGEAYGRLLTEPATKPQPKMPYSAI
                     FVPDGSFKTWAEMTDEERNKISHRGKAFKQVADFLKKEVIK"
     gene            3716..4117
                     /gene="msrB"
                     /locus_tag="VI981_02365"
     CDS             3716..4117
                     /gene="msrB"
                     /locus_tag="VI981_02365"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004216835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY4479173.1"
                     /translation="MKEKSMPKTQSEWKDKLTEEQYRVLREKGTEAPFSGKYVNEKRA
                     GMYKCAACGNELFASDAKFDSGTGWPSFDEALPGAVRLRDDLSRGMRRTEVVCARCGS
                     HLGHVFDDGPKNKSGKRYCMNSVCLDLEEKK"
     gene            4117..5217
                     /locus_tag="VI981_02370"
     CDS             4117..5217
                     /locus_tag="VI981_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4479174.1"
                     /translation="MQFAFLFVTAALQLLVFFVHWLLYESIKIFGGFGSPLLAWVIVL
                     LSMSFLPASALVHSVNNSFSRWMYRVAATWMGMLVYLFSAVLCTAFLFWISNLFGFAV
                     PAAFILYTLFFVALLVSFHGMSRATRPVVRGIRVKLPHLRNEWAGEKIVFLSDTHLGQ
                     IHGTAYARTVATLIEVEKPVAVLFGGDLFDGVACDTHEYAAEFAKIKTKFGIFFVSGN
                     HESYGDETGYFRALNDAGIRILKNEIVNLDGLQIVGIEYATGEDENKYKAALSKLQIQ
                     TDVPSILMKHAPTLIATTEESGVNLFLAGHTHHGQQYPFSYLTRWVYRGFDYGLKQYK
                     NLTVYTTSGAGTWGPPIRFMTQSEIVVIEFEK"
     gene            5954..6226
                     /locus_tag="VI981_02375"
     CDS             5954..6226
                     /locus_tag="VI981_02375"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4479175.1"
                     /translation="MTEESIEKIGKALSDKTRVKLLEEIKCHEGLCCMEAQNCTSLTQ
                     PTVSHHIKILIDSGLVKAERAGRKVKLKINKKELDKFTNYMNKLVV"
     gene            6238..6441
                     /locus_tag="VI981_02380"
     CDS             6238..6441
                     /locus_tag="VI981_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY4479176.1"
                     /translation="MKKLYRSRNNNVISGVLGGVAEYFGVDPMIVRLIFLFVVFLTGV
                     FPALIVYFIAILAVPFEDRSKTS"
     gene            6478..>6704
                     /locus_tag="VI981_02385"
     CDS             6478..>6704
                     /locus_tag="VI981_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479177.1"
                     /translation="MKKRTLITIIIALVIILSFAMFYPYKYMQTDESETQDEQENKTF
                     ILSPGQSTEINGVAFTLVKIAGDSRCPLDVT"
BASE COUNT         1768 a         1442 c         1576 g         1918 t
ORIGIN      
        1 ccatatttct ttacatgaga cgacctgcat ttgggacaaa ttcttccatt tttttaaaca
       61 tgtttgtata ttttatgaaa agtatcaatt agtagagccg cagtatatca aaagtagcaa
      121 cccaaaaagg cctttagctc gaaaattctt tagggtaaaa tcaaaaacct tgcatattcc
      181 cgcgatcgcg ggaatatgca aggttgggat ttgggcgata tcttctagag acctacttta
      241 atctgcgccg ttacttcaac ttcgagtgtc ggtttgtctt taaccttgcc gtagccacat
      301 tgtggtcccg ggtcgtatac tgttgaaccg tcagtcgcta cgacagatcc gcgcgccgca
      361 tgatagtgat ctgcgccgca accgacttca tcttcaatta ttaactgatc tatgggaaga
      421 tattttccgt tatagttgat tactttctct tttgcttttt gagtgggtgg cggtgtagtt
      481 acgggcggtg gagttgccga cggtggcggg gtgccgactt gtacctttgt gtctgtcttt
      541 acgtcaacgc agtttatttt ccccttctct ccatttttgc cggcaggttt tccttttccc
      601 cctttgccgc cgttgcctgg gccgcagccg tcaccgccat tgcctccatt acctccagtt
      661 tcatttgtaa tctttccgtc gtcaccatca gttgaagttc ctttgccggc tgggccctct
      721 tttgacgagg catcacctcc tgcgccgccc gttccaccct gtggaactcc ttggcagctg
      781 ccgccgactc ctcctgttgc gccttgtgag tcagtgttac cgccatttcc tccgtttgca
      841 tcagcctgtg ctccgggaat agtgactgag gcatcgccac cctttccgcc agttgccgtt
      901 ccattgccgc ctttcccgcc gccgcactta caggcgtttc cgtcaccgcc ttttcctggc
      961 tgtgcggctg cgtttccgcc ccagccgcct tttacttcgt caacagtgac atttgcaatt
     1021 ccggaaactg caccgattgc tgcaagttcc tttttgtttt ttccgccatt tccgccagtt
     1081 gcggtagcat cgcctccttt tacgcccgga catccggcta caccgtcttt tccatgcgcc
     1141 gtagcgtcac ctcctgtgcc tccaactccg gggacgatat gaaatgaagc gatctcaata
     1201 ctgcctcggg cgttcatttt gaagtttccg gcttcgccgc cgtcaccgcc cgttgcaatt
     1261 cccggatcgc aatctatttt agtttctgcc gtgccgccat gcccgccgtt tccaagctca
     1321 agtctgaaat tatttatcgt aatattgtta gcgtccactt tcatgcggaa cgcattttca
     1381 ccgcgagcgc cgcgagcgac acaattttta ttttcatcgc tgtcaccatc acgaccagcc
     1441 ggaccgacta agtgaaaatc tcgaagctct acatttccct cggctccaaa tgcgaagtag
     1501 agaataattt tatgcactcc ttttgggggt ttcggaatat cgagtcccgc gggcggattt
     1561 ccgccgtctc ccacgtacca gttaccgctt atgactatat tatccaaagc gtttccgtct
     1621 ttatcgcgcg gagtttgggc ataggcggtt tgagctataa gtgtgcgaac gatactgtct
     1681 ttttttggag ttgtactcac gcttgcgctt ccacttacgt ctactgtcgc atttgcctca
     1741 acgaagggac caatacgcgg tccggctcca gtgttcttac cggcatcttc gtacgctgca
     1801 tcaatctccg cttgtgtatc aagcgcgagg tcaaaatcgc gcacgacttg aatgtggctg
     1861 tttgaggaga taacggcatc agatccgaat gtcacgcctt ttccaactat taaaataatc
     1921 ccggacgtct cgcttggagt gtcttccgcc aagatgcacg aaagtgttcc gtttatctcg
     1981 gcatttccaa ggacagttat gacaagcggt ccgccgtcgc atgttagttt ggaatccttt
     2041 ttgagcgaaa gatcaccttt gacggttaat ttcgcattat ttttgaatat aatttcttgg
     2101 ctgggagtgt aattttcctt aacaacaaga tcgctttcta cgatctgttg tcctctgttg
     2161 cctccaatcc gggtgagaag cgcaataaca ataataacga ttacaagaga acctacgatt
     2221 tttttagtca tgtctccgcg atttagtagt aattaaatga aatactatac ctgattcccg
     2281 cgctgtcaat tatgacgttt ttgatacaat gattctgtga agtagaaatt gctgttttcg
     2341 gtggaggctg tttctggtgc accgaagcag tgttttccgc cggaggcgga tccgcctatg
     2401 gcggaaaaat attcaaagga atcatttctg ttgagcccgg atacgcgggt ggcctcgaag
     2461 atacggcaac atataaacaa gtctttggag gaacgaccgg tcatgtcgaa gtcattcgga
     2521 ttgaatttga tccttcaaga atttccttta gagagttgct gacggtattt tttggctctc
     2581 atgatccgac cacactgaat cgacagggag gcgatgtcgg cgctcaatat cgctcagtca
     2641 ttttttatac gacgcccgcg cagaaaaatg aatcggacaa attcatcaaa gaattgaacg
     2701 attcaaatga aatgggcgag cctatcgtga cagcagttga accgcttgtc gaatttattc
     2761 ctgcagagga ttatcataaa gattattacg ctagaaacca agagcaaggg tattgccaga
     2821 ttgttatcaa tcctaaacta gaaaaagtcc agcagaaatt cgaagcacta ataaaaaaaa
     2881 gcatctgaaa ttatgaagta tgatttttgt accatacaac tataaaatgc tatagcattt
     2941 tatagttgta tggtgccttg gtgtttaagt attgcgatgc tggtaaggac ggtttgatta
     3001 ttcaatgatt tagcttggcc agagtcataa ttggcatagc cagaaaggaa acttcgactg
     3061 aaggctatat ttctagaatt cagggtacac ttagaaaatg atgaatattg tacttgcgac
     3121 aaataatcca agtaaggctg aacatatcaa atctcaattt gcgaagcttc cgattgagct
     3181 taggacattg caagagtgcg gtattacaga agagccggtt gagaacggaa aaacgctcga
     3241 agagaacgct cttataaagg ctaaatttgc gtgggagcgg actggtaaat gctcaatcgc
     3301 ggacgacacc ggaattttta tcgacgccct aggtggcgcc ccgggcattc attcgggccg
     3361 ttttgcgggc gttggcaaaa caatagagga aggacaagct tttgttattg aaaaaatgaa
     3421 agacgtgaaa gagcaagatc gatcagcggt ttttagaacg gttgctgtag tttttgaggc
     3481 tgacggaacc cactccgttt ttagaggcga agcatatggg cgattgttga ccgagcctgc
     3541 aacgaagcct cagcctaaaa tgccatacag tgctattttt gtacctgacg gaagttttaa
     3601 aacgtgggca gaaatgaccg atgaagagag aaataagatt tcacatcgcg gaaaagcatt
     3661 taaacaagta gccgattttt tgaaaaaaga agtaataaaa tagtagaata gccaaatgaa
     3721 agagaagtca atgccaaaaa cacaaagtga gtggaaagat aagctgactg aagagcaata
     3781 tcgcgtgttg cgcgagaagg gaacggaagc gcctttttct ggaaaatatg ttaatgaaaa
     3841 acgtgctggt atgtacaaat gcgccgcgtg cggcaatgag cttttcgctt cagatgctaa
     3901 attcgattca ggcacgggtt ggccgtcgtt cgatgaagcg ttacccggag ctgtgagatt
     3961 gcgtgatgac ttatcgcgcg gcatgaggcg gaccgaagta gtctgcgcaa gatgcggttc
     4021 gcatttaggt cacgtatttg acgatggacc gaagaataag tcggggaaac ggtactgcat
     4081 gaactcggtc tgcctcgacc ttgaggaaaa aaaataatgc aatttgcatt tctctttgtt
     4141 actgccgcgc ttcagttact tgtgtttttt gtacattggc ttttatatga atccataaag
     4201 atattcggtg gttttggatc tccacttttg gcttgggtaa ttgtgttgct atcgatgtcg
     4261 tttcttcccg catcggcgct tgtacactcg gttaataaca gtttttcaag gtggatgtat
     4321 cgcgtagcgg ctacgtggat gggaatgctc gtttatctgt tttccgccgt tctatgcaca
     4381 gcatttctat tctggattag taatcttttt ggttttgctg ttcctgccgc tttcatttta
     4441 tatacacttt tttttgtggc ccttttggtc agtttccatg ggatgtcccg ggccactcgg
     4501 cctgtcgtgc gtggaattcg ggttaaactt ccacatctac gaaatgagtg ggctggtgaa
     4561 aaaattgtgt ttttgagcga cacgcatttg gggcagattc atggcactgc gtacgccaga
     4621 actgtggcga cccttattga agttgaaaaa cctgtggcag tgctctttgg gggagatttg
     4681 tttgatggcg tagcgtgtga cacgcatgaa tatgcagcgg agttcgcgaa aataaaaacc
     4741 aagtttggaa tcttttttgt cagcggtaat cacgaaagtt acggcgatga gacgggttac
     4801 tttcgggcgc tcaatgacgc cggaataagg attttaaaaa acgaaatcgt gaatctcgat
     4861 ggattgcaaa ttgtaggaat tgagtatgcg accggtgagg acgaaaataa gtacaaagcg
     4921 gcgctttcga agctccagat tcaaacggat gtaccttcga ttttaatgaa gcatgcacct
     4981 acactcatcg ctacaactga agagagcggc gtgaatcttt ttcttgcagg gcatacgcat
     5041 cacggccagc agtatccctt cagctatctg acacgatggg tatatagggg ttttgattat
     5101 ggtctgaaac aatacaaaaa tctcactgta tatacgacga gcggggccgg aacgtggggc
     5161 ccgcccattc gttttatgac tcaatctgaa atagtcgtga tcgagtttga aaagtgacac
     5221 atagagcttg ttgtatggta aaacagagcg tgaaattaac tagtatactc atccgtcttg
     5281 agctatggtc gagacattac tcattgtttt gacaattata tatatggaaa aggatcaaga
     5341 aaaaaagact tctttagaaa aagaagaaga agaggaaaaa aaggacgaaa aaacggcgga
     5401 ttttttcagt aatatggatc aatgacgctc ggatatatct ttttacttgc acgataaaat
     5461 tctgcggaat ttttctctgc tcaccgaagc accattctgg tgctcggctc cgcaaggcac
     5521 ttaacaagaa tatccgtcgc cagccggaaa gtggaacgaa tcccgcacag gcgggattcg
     5581 tgggtattaa ttttttgaaa gacaacgctc gtagacacgc tcatgtggag gcacaaccgc
     5641 gcgaaaaacg cggttgtgct gaacaaatgc tctcaaaaag agaagagtca tatggaatga
     5701 agaaagtcgg tggagtttcg atgactcgca cggatctata ttgatctatc cgaatcgttt
     5761 ctgacgacaa gcatttccat cgatacattt gaataaatgg cggaaatacg tatagttaat
     5821 gaattaagag actacaaggt gacttctgat tgattttaga gagtagttgg cctactttgc
     5881 caataatcga aaagaaatta gattagcttg agatgaactc ttatagttgt tagactcctt
     5941 aaaaatcaat catatgactg aagaatctat agaaaaaatt ggtaaggcgc tttccgacaa
     6001 aactcgggtc aagctacttg aggaaataaa gtgtcatgaa ggactttgtt gtatggaagc
     6061 ccaaaactgt acgtcgctaa cacagccgac tgtctcgcat catattaaaa ttttgatcga
     6121 ttccggactc gtcaaggctg aacgcgcggg tagaaaagta aaacttaaga taaacaagaa
     6181 ggagctagac aaatttacga actatatgaa taaactcgtt gtttaatttc taacgggatg
     6241 aaaaaattat accgaagcag aaataataat gttataagcg gagtactcgg aggcgttgcc
     6301 gaatatttcg gagtcgatcc gatgatcgtg cgcctcattt tcctgtttgt tgtttttctg
     6361 actggagttt ttccggcgct gatcgtctat tttattgcaa ttctcgcagt tccgtttgag
     6421 gatcgaagca aaacctcgta agtttcgtac tgaaggggcg tctagtattc gaactatatg
     6481 aaaaagcgta cgcttataac cattataatt gctctcgtaa ttatcctgtc ttttgcgatg
     6541 ttttatcctt acaaatatat gcaaacggat gaaagcgaga cgcaagacga acaggaaaac
     6601 aaaactttta ttttatcgcc cggtcaatcg acggaaataa acggggtggc gtttacactc
     6661 gtcaaaatag cgggggatag ccgctgtccg ctagatgtaa catg
//
LOCUS       DATDSU010000069         3132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_572515, whole genome shotgun sequence.
ACCESSION   DATDSU010000069 DATDSU010000000
VERSION     DATDSU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572515"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..489
                     /locus_tag="VI981_02390"
     CDS             154..489
                     /locus_tag="VI981_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479178.1"
                     /translation="MKKIVANLLVRNPYSAVRHCFTSKVDLPEFPKKHERVWLSVGNG
                     LRQFRVVKRWDMQQGEDCVEFDFKRCLLVTSEFGIWLALEIDSRWKLFHRERYEKIIE
                     ERYLAFTRA"
     gene            complement(547..1698)
                     /gene="ftsZ"
                     /locus_tag="VI981_02395"
     CDS             complement(547..1698)
                     /gene="ftsZ"
                     /locus_tag="VI981_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY4479179.1"
                     /translation="MAQITPEVEAFARIKVVGVGGSGKNAVNHMVNSKVRGVEFIVVN
                     TDAQDLHNSQAKRKIHIGKNLTRGLGTGMNPELGKRAAEETKEEIQEAIKGADMVFIA
                     CGKGGGTGTGAAPVVAKVAKEMGILTVGVVTKPFFFEGQQRMKFAEIGLEELRKSVDA
                     LIVIPNDRILSIVNKETTAQQAFAMCDEVLKQAVEGISDLITTPGIINVDFADVRSVL
                     ENAGSALMGIGTASGEKRAEEAARLAINSPLLDISINGAKAVLFSIAGGEDLTMFEIQ
                     DAAKVITESIDPNAKVIFGTVRDEKLKKNEIKVTVIAAGFPEASFKKNLFHQEEEKKG
                     KIFNSISFSSKDKEPKSEDKKMTPVAASEETDDDWGAVPAFLRRAKSQK"
     gene            complement(2122..>3132)
                     /locus_tag="VI981_02400"
     CDS             complement(2122..>3132)
                     /locus_tag="VI981_02400"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEY4479180.1"
                     /translation="DAAKSIRSAVRQAEKMADVEVKKAYLAIGGIGLGAVVSSGSTMI
                     TKADGEVSALDIEQALASSENEIPRPLIINRKIIHTVPLQYKIDGISTLGKAEGLKGS
                     KLEVKTLFITCLEHHINDLVQAAEEAGIQVEDVIAAPIAASMVTLTKLQKVAGCALAN
                     IGAETVSISVFENNLPVSLEVFPIGSNDITNDIALGLKIPLEEAEVIKVHNLNNSPYS
                     KKKIEEIIGARLSDIFELIEAHLKKIGRNGLLPAGIVITGGGAGLTEIDNFAKLSLNI
                     PSRVGIVHVPVAKNTIKDATWAVVYGLTIIGFTSGEDDSTGLTFLKTKKSIVNWFRQF
                     LP"
BASE COUNT          764 a          702 c          673 g          993 t
ORIGIN      
        1 tccgcctgag gcggatccgc ctttggtgga agtgttaagg atttagtaat aacaatatga
       61 atgtagaaaa ttatacacgg gatgcgagca taacccaaac ttgattcttg gcaacaagct
      121 ggtaacctta cgttagaaaa aacaaaacct cttatgaaaa aaatcgttgc gaatcttctc
      181 gttcgtaatc cttactctgc agtacgtcat tgctttactt ctaaagtcga tttgcccgag
      241 tttccaaaaa aacatgaacg agtatggctt agcgtgggta atggtttgcg tcaattcagg
      301 gtagttaagc gctgggacat gcaacagggg gaggattgcg tcgaatttga tttcaagcga
      361 tgtctgttag taacgagcga gtttgggatt tggctcgccc tcgagattga ttctcgctgg
      421 aagctatttc acagagaacg ctatgaaaag attatcgaag agagatattt ggctttcact
      481 cgcgcttagc gtcacccact aatcccatgt gggttttttg tttatctata agctaacagc
      541 taaaagctat ttttgcgatt tagcccttcg gagaaatgca ggcacagcgc cccagtcatc
      601 atctgtttcc tctgacgccg caactggcgt catttttttg tcttccgatt taggttcctt
      661 gtcttttgag gagaaactta ttgaattgaa aattttccct ttcttttctt cttcctggtg
      721 gaaaagattt ttcttgaacg acgcttccgg gaatcctgcc gctatcacgg tcacttttat
      781 ttcgttcttc ttcaattttt catcgcgtac cgttccgaaa ataacttttg cattcgggtc
      841 gattgattca gtgatgactt ttgccgcatc ttgaatttca aacatggtaa gatcctctcc
      901 gccagcaatc gaaaagagca ctgccttcgc gccgtttatt gaaatatcta aaagtgggga
      961 attaatagcc agtcgagctg cttcctccgc tcgcttctct ccgcttgccg ttccgattcc
     1021 catgagggcg gagcccgcat tttcgagaac agagcgaacg tcggcgaaat ctacgttgat
     1081 gattccgggt gtcgtgatga gatctgaaat accctcgact gcttgtttta aaacttcgtc
     1141 gcacatggca aacgcctgct gcgcagtcgt ctctttgttg acgattgaaa ggatgcggtc
     1201 attcggaatt acgatcagcg cgtccaccga ctttcttagt tcttcgagac cgatttcggc
     1261 aaatttcatt cgctgttgtc cttcaaaaaa gaatggtttt gtgacaacac caacggtcag
     1321 tatgcccatc tcttttgcaa cttttgccac gactggagcg gcacccgttc ccgttcctcc
     1381 gcctttaccg caggcgatga acaccatatc ggcacccttt attgcttctt ggatttcttc
     1441 ctttgtttct tctgccgcgc gttttccaag ttcagggttc atacctgttc caaggccgcg
     1501 ggtcaaattt ttgccgatat gaatttttct tttagcttga gagttatgaa ggtcttgggc
     1561 gtccgtgttt actacaataa actccactcc gcggaccttg gaattgacca tgtggttgac
     1621 cgcattcttg cctgaaccgc caacgcccac gactttaatc cgtgcaaacg cctctacttc
     1681 tggtgtaatt tgagccattt tatatattta aaatttaaca gttacgagta tacatgcgcc
     1741 cctaactggc gtattcctgc gcaatgattt acccatgata tcagagggtc aaaaaaagaa
     1801 aacttgacct tttatgactt tgttatgtcg ttttgcgtgc tttctgaccg cccggtatcg
     1861 tattttgttg tccttgagcg aggagtgtca ttttgaaaaa acttattatg ttgctgggtc
     1921 ttttctcttc ttttgtacta agcgagcgtt catcttttta gaaacatatg cgggaggtaa
     1981 tctggaaatt acggagactt gtatgaaaaa tcgaactgtt tgagtctagt ctgaggcgag
     2041 ttttcaattt ttcataaaca agacgacgat aatttgtgat taccgaagca tcgtttttta
     2101 aaagttgaac gcgagcgttc tttatggcag aaactgcctg aaccaattaa caatggattt
     2161 tttagtcttc aaaaaagtta gaccagttga atcatcttct ccgcttgtga agccgataat
     2221 agtaagtccg tagacgactg cccatgttgc gtcttttata gtatttttcg caactgggac
     2281 gtgaactata ccgactcggg agggaatatt gagcgaaagt tttgcaaagt tatcaatttc
     2341 agtaagtccc gcgccgccac ccgttatgac aataccggct ggtaacagtc cgtttcggcc
     2401 aatttttttc aagtgtgctt cgataagttc aaaaatatca ctaagccgtg ccccaattat
     2461 ttcctcaatt tttttcttcg aatatggtga attatttaaa ttatggactt ttattacctc
     2521 cgcctcttcg agaggaattt taaggccgag tgcgatgtca ttggttatat cgtttgatcc
     2581 gattggaaaa acttccaaag aaacgggaag attattttca aaaacactta tagacaccgt
     2641 ttcggctcct atattcgcaa gtgcgcagcc tgctactttt tggagtttgg taagcgtcac
     2701 catgcttgcc gcgatcggtg ccgcaatgac atcttccact tgaatgcctg cttcttcggc
     2761 ggcctggact aaatcattga tgtgatgttc aagacacgta ataaaaagag ttttaacctc
     2821 aagtttcgat ccttttaaac cctcagcttt tccgagtgta cttatgccgt ctatcttata
     2881 ttgaagcgga acggtatgaa tgatttttct attgataata agcgggcggg ggatttcgtt
     2941 ttcgctcgag gcaagggctt gctctatgtc gagtgcgctc acttcaccgt cagctttcgt
     3001 aatcatggta gaccctgacg aaacaactgc tcctaagccg attccaccga tagcaagata
     3061 tgcctttttt acctcaacgt ctgccatttt ttccgcctgg cgaacggcag aacggatgct
     3121 ttttgcggcg tc
//
LOCUS       DATDSU010000070         3844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1578562, whole genome shotgun sequence.
ACCESSION   DATDSU010000070 DATDSU010000000
VERSION     DATDSU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578562"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..1033)
                     /locus_tag="VI981_02405"
     CDS             complement(422..1033)
                     /locus_tag="VI981_02405"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY4479181.1"
                     /translation="MHKEILTDEQVALLPLLKYMRKEYGLVGGTAIALHIGHRESIDF
                     DLFSNKEFRTSALKKKIERVVKISHVIVDKSGELTFLVGDVKVTFYHYPFDIAYTEDF
                     EGIIKMPNLVTLAAMKAYALGRRAKWKDYVDLRFIMKGYHSLQEIVQQAESIFGTQFN
                     SKLFRIQLAYFDDVKYDEKVVFMPGVEVSEKKIKKELVEFSLE"
     gene            complement(1026..1289)
                     /locus_tag="VI981_02410"
     CDS             complement(1026..1289)
                     /locus_tag="VI981_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479182.1"
                     /translation="MTIHDFIKERKHLIWWVKDYDKLKAESIVEATLNYGDWDDVQTL
                     IKILGIKKVAKIFREKSKPSKMGRQNYRAEVKHYFALYFNKYA"
     gene            complement(1276..1950)
                     /locus_tag="VI981_02415"
     CDS             complement(1276..1950)
                     /locus_tag="VI981_02415"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY4479183.1"
                     /translation="MTKKILTASQRKVIKKISQAKDINDQFVLTGGTALAGFYLHHRF
                     SEDLDLFSQEEVDPEMIHVFLTSLRKELAITDIDFQHTFNRHIYFLTLKNSEILKMEF
                     TYFPFSPIENPLQQEGIRVESIIDLAVNKLFTIYQRLTARDFIDLFFILQTYPFSLSD
                     ITKKAKIKFDWHIDPLQLGTQFIQAQKLKDYPRMVQPFNHNHWKKFFLKEAEKLEKDV
                     FDYDHS"
     gene            complement(1907..2137)
                     /locus_tag="VI981_02420"
     CDS             complement(1907..2137)
                     /locus_tag="VI981_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479184.1"
                     /translation="MYNWSTDTTALKKGKKQYAKWRLEQLINFGIGKEKLSMQELRKY
                     WDTLNIDPHRREFLNLFIHDKENSYRIPKKSH"
     gene            2872..>3844
                     /locus_tag="VI981_02425"
     CDS             2872..>3844
                     /locus_tag="VI981_02425"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY4479185.1"
                     /translation="MRQKLSFQLYLIKTGDERKDKGNGTAEALLVEITDLDQTGPYEL
                     YCENGIMAEGDRAAIQKFLEAEVAKQPTAEEELLAETQDELATRTSELRAVQGTARQI
                     AGERDVAVAQRDESRLGHDLIVNKLAEVAKANGELQETFEQTYEKLTDAQADVLSAKS
                     ELRTANDALAWMRARMTNFLGMIGLTANLINDSNADHNVTARIYEIKQHTAWYCGVTG
                     LVVGGIIVGAIWLFTTAFDGCAGKPNNQPPTLSVTPTATTVEIGKVATVSYDVTDPDD
                     KKHDVEVVTSPKIGKVTVDQESKTLTYTSDGISLGDDTFQVVAKDKES"
BASE COUNT         1116 a          872 c          807 g         1049 t
ORIGIN      
        1 atctttaaaa ttttgcggaa aatggttaaa gatgttcaaa aaaacatttc cgctatcacc
       61 gatcaagggg tcaaaagcgc cacgatgaca agcactttcc aatcaccatg gttggcgcca
      121 gtgattcgct atggtgactc gcgaagctga cggcatgtca ctgtttaggc gacagctggt
      181 gcgaatgaaa aaccagcaaa agctgatttc acgcgcgcga caagattctt ggcatttgtc
      241 ggcttggtta agagattaac gagcgatcaa ccacgctcga atggccaaaa tatgaaacaa
      301 tctgcttatc gaataatacc atccccatgt ttgtgggcta ttcgtaaaga ttcgtgatgt
      361 attcgtggcg aagtataata aaaaacaacc ctgccgtcaa gcccatcgag caaaaaatag
      421 gttattccaa actaaactca actaactctt tttttatctt tttctcactc acctcaaccc
      481 cgggcataaa aaccaccttt tcatcatatt tgacatcatc aaaatacgct aattgtattc
      541 taaacaattt actgttaaat tgtgtgccaa atatggattc agcttgttgt acaatctcct
      601 gcaacgaatg ataacctttc ataataaaac gcaaatcaac ataatccttc cactttgccc
      661 gccgacctaa agcgtatgct ttcatcgcgg ccaacgtgac taaatttggc attttgatta
      721 taccctcaaa atcttccgtg tatgctatat caaatggata atgataaaac gtaactttta
      781 catcccccac taaaaaggtt aattctcctg atttatcaac aataacgtgg cttattttta
      841 caactcgttc aatttttttc ttaagagcgg atgtcctaaa ctccttgttt gaaaataaat
      901 caaaatcaat tgattctcta tgaccaatgt gtaaagcgat agcagtacct ccaaccaacc
      961 catattcttt cctcatgtac tttaacaacg gcaataatgc tacctgttcg tctgtgagta
     1021 tttccttatg catatttatt aaaatacaag gcaaagtaat gctttacttc cgcccgataa
     1081 ttttgccgac ccatttttga gggtttggat ttttcacgaa aaattttagc aacttttttg
     1141 atgcctaaaa tcttaattaa tgtctgcacg tcatcccaat ccccataatt cagagttgcc
     1201 tccacaatgg actctgcctt tagtttatcg taatccttta cccaccaaat aagatgtttt
     1261 ctttctttaa taaaatcatg aatggtcata gtcaaataca tccttttcta atttttctgc
     1321 ttctttcaaa aaaaatttct tccaatgatt atgattaaac ggttgtacca tgcgcggata
     1381 atctttcaat ttttgcgcct gaataaactg tgtccccaat tgaagcgggt caatatgcca
     1441 gtcgaatttt atctttgctt tcttcgtaat atctgaaagt gaaaatggat acgtctgcaa
     1501 aataaaaaaa agatcaataa aatccctggc cgttaagcgc tggtatatgg taaaaagttt
     1561 attcaccgct aaatctataa tactctcaac ccgtatcccc tcctgctgga gaggattttc
     1621 aatcggagaa aacgggaaat aggtgaattc cattttgagt atttcactgt ttttaagcgt
     1681 caaaaaatag atatgacggt taaaggtatg ctgaaaatca atgtcagtga ttgcaagttc
     1741 ttttcgtaaa gatgtcaaaa aaacatggat catctcaggg tcaacttctt cttgtgagaa
     1801 caaatcaaga tcttcggaaa accgatgatg caaataaaac cctgccaaag cggtaccgcc
     1861 ggttaaaaca aactggtcat ttatatcttt agcttgagat atttttttaa tgacttttct
     1921 ttgggatgcg gtaagaattt tctttgtcat ggataaaaag gttaagaaac tcccgccggt
     1981 gaggatcaat gttcagtgta tcccagtact tgcgaagttc ttgcatagag agcttttctt
     2041 tgccaatgcc aaaattaatt agctgttcaa gtcgccattt agcatactgt tttttacctt
     2101 ttttcagcgc tgttgtatct gtagaccaat tgtacataaa atgtattcta aaccacccct
     2161 atcccctcct tttagaggaa ggatatttac ctgtattata gcatagaatt gaaattttag
     2221 acaataacgt attatcaaaa atagggctta ttttagctaa tctctggttg ttgacaataa
     2281 ttacaagata tggtactatg gcatgttttc tgaaaactga agttgagagt tgatcaaacg
     2341 tgcggtgtaa ggccttgtgt cgtacatcgc gcgctctgat gagctcttaa tacatgtaag
     2401 aatagtacaa atcacgaaaa aactgataca tccaagatat taagcgattg tgagttgcgt
     2461 gacttgtact tattttcatt caattggtgg gcgacctcac cctaaccctc tccttataaa
     2521 ggagagggaa acgaatcccc ccttaagagg ggaacgacgc gcctccttcc ttgaggaagg
     2581 tggcacccgc agggtgacgg aaggtgttac cactaattga cttgcccagt cctcgtgact
     2641 ggggctcact tcatatctga taatacacaa ccgatattcg tatcatttgc atgaatttgt
     2701 atatttgcgt cgcatttgtc agataaaaag tgggactttc gtgagcgtgg gtatgttgcc
     2761 tgcgcttaat gcgtcgttca tcgacaacga gttgaggtta cgttgtcccc tacctcaact
     2821 acacttcaca acgcatcacg caagtgatgc gctctcgtaa ggagcttcaa aatgcgtcaa
     2881 aaactgagtt ttcaactcta cctcatcaag accggtgacg agaggaaaga caaaggaaac
     2941 ggaactgcgg aagcgttgct cgtcgaaatc acggaccttg accaaacggg tccgtatgag
     3001 ttgtactgcg agaatgggat catggccgaa ggcgaccgcg cagcgatcca gaaatttctc
     3061 gaggcggagg ttgccaagca accgacggcc gaagaagagc ttcttgccga gacgcaagac
     3121 gagctcgcca caaggacgag cgagctacgt gctgtccagg ggacggcacg gcagatcgcc
     3181 ggcgagcgcg acgtggcggt tgcccagcgg gatgaatccc gcctcgggca cgatctgatc
     3241 gtgaacaagc tcgccgaagt cgcgaaagcg aatggcgagc tccaggagac gttcgagcaa
     3301 acgtacgaga agctcaccga cgctcaggcc gacgtgttgt cggcgaaaag cgagctcagg
     3361 accgcgaacg atgccttggc ttggatgcgt gcgcggatga caaactttct tggcatgatc
     3421 ggcctcacgg ccaatctcat caacgactcc aacgcagatc ataacgtaac agcaaggatc
     3481 tacgaaatca aacaacatac ggcctggtac tgcggtgtca ctggtctcgt cgtcggcggg
     3541 atcatcgtcg gcgccatctg gctcttcacg acggcgttcg acggctgtgc ggggaaaccc
     3601 aacaaccagc cgccgacact gtcggttacg ccgacagcga cgacggtcga gatcgggaag
     3661 gtcgcgacgg tgtcctacga tgtcaccgac cctgacgaca agaagcatga cgtcgaggtt
     3721 gtgaccagtc ccaaaatcgg gaaggtcaca gtcgaccagg aatcgaagac tctgacgtac
     3781 acctccgatg gcatcagcct cggggatgat acgttccagg tcgtcgcaaa agacaaagag
     3841 tcca
//
LOCUS       DATDSU010000071         3798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_230196, whole genome shotgun sequence.
ACCESSION   DATDSU010000071 DATDSU010000000
VERSION     DATDSU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230196"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..980)
                     /locus_tag="VI981_02430"
     CDS             complement(129..980)
                     /locus_tag="VI981_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY4479186.1"
                     /translation="MKKYFLIAIIVALSSTFFVWRPSVIQDVKHTVGVARDNAELFVT
                     EILPKKLVNPEPLKIFRSVNGTLTVSGIFAETNNERIRNGVAALSLDGTLSSVAAKKA
                     DDMFARQYFEHDSPDGKGAADLADAVGYDYIVIGENLALGNFKDDKDLVRAWMESPGH
                     RANILNKRFSEIGIAAKKGTYEGKNVWLAVQTFAVPASLCPAPSPSLRVKIDASQIEL
                     KKIETELALKKDELESYRGNPSEYNKRAAEFNALVATHNALVEETKKSVALFNAEVRA
                     FNTCIEG"
     gene            complement(1054..1239)
                     /locus_tag="VI981_02435"
     CDS             complement(1054..1239)
                     /locus_tag="VI981_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479187.1"
                     /translation="MTHYVCSGDCGGESETPKVCDVEGCSKEGENLSVCACADGLHAG
                     VVPPPDEPILEDTDEEE"
     gene            complement(1510..2301)
                     /locus_tag="VI981_02440"
     CDS             complement(1510..2301)
                     /locus_tag="VI981_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4479188.1"
                     /translation="MIRKKPAAKEKQKEESKETSWGAVSDWYDDLLGSAETFQAEVIL
                     PHLLRIIEPRTGLQIADVACGQGFFSRAFSTGGASVAACDISKALITLAEKKGGSVKY
                     EVASADHLPWKDGSFDIAALILAIQNIAPLYETMAECARVLKHGGRLIVVLNHPAFRI
                     PKKSSWGFDDVLKIQYRRLDEYASESKAEIKAHPGQQSSAVTISFHRPLQTYFKALHK
                     AGFVVERLEEWISNKKSESGPRKLIEDKARKEIPLFMMLECRVTP"
     gene            complement(2252..2620)
                     /locus_tag="VI981_02445"
     CDS             complement(2252..2620)
                     /locus_tag="VI981_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479189.1"
                     /translation="MAQKKNKILVGAGIATLAALAGAYLLYGDKDGAARRKKIRGWLI
                     KAKGEIIERLEKLEAVNEDVYNRIVEEVVKKYKKAKNVDPAEIQALVKDARAQWKNIR
                     KIFSHDTKKAGRKRKAERRK"
     gene            complement(2601..2906)
                     /locus_tag="VI981_02450"
     CDS             complement(2601..2906)
                     /locus_tag="VI981_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479190.1"
                     /translation="MESIIHADIFFFISTVVLITIGVLGGVALVYVILILHDAKHIST
                     EVRKETNNIATLVRELCAEIRTTGFKMSSILRFIRAIFSSRRFVKSKHSKGNGTEEK"
     gene            complement(2961..3617)
                     /locus_tag="VI981_02455"
     CDS             complement(2961..3617)
                     /locus_tag="VI981_02455"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEY4479191.1"
                     /translation="MRRNISILIPIVILIALALLLYYKMRPTDRYMVKNVTNFEECAE
                     AGNPILESYPRQCKTKDGKSFTEDINQVTVNTFEDCVKAGFPILLSYPRVCDSETASF
                     IEKPLTRSGQDLIRLMSPHLGGIVPSPLTIGGEARGNWFFEASFPVEILDSNGKQIAI
                     APAQAQGDWMTENFVPFRATLVFKTVPTTATGTLVLHKDNPSGLPEHDDELVMPIRFK
                     "
     gene            complement(3614..>3798)
                     /locus_tag="VI981_02460"
     CDS             complement(3614..>3798)
                     /locus_tag="VI981_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479192.1"
                     /translation="VSVEKGGTHAALTVTSTDTAEFTIESRSYTVSIKDVLPAKETGG
                     EIDQKNYKITFEITKK"
BASE COUNT          976 a          921 c          761 g         1140 t
ORIGIN      
        1 cgcggcttct aacggggtaa aattctcaat gttcaattac caatgatcaa tgaattcata
       61 gaacgaatca ttgcccaccc taggcgggtc cgcctttggc ggaaaaattg tgaattgata
      121 cttgaaaatt atccttctat acaggtatta aaggctcgga cttccgcatt gaaaagtgcc
      181 acactctttt ttgtttcctc aacgagtgcg ttatgcgtag cgactaaggc attgaattca
      241 gcggcccgtt tattatattc cgatgggttg ccgcggtagc tttcaagttc atctttttta
      301 agtgcaagtt ccgtttcgat cttttttaat tcaatctgac ttgcgtcaat cttcacgcga
      361 agcgagggcg agggcgccgg gcagagactc gcaggcacgg caaatgtttg gacggcaagc
      421 cagacatttt ttccttcata agtccctttt ttcgccgcaa tgccgatttc gctgaaacgt
      481 ttgttcaaaa tgtttgcacg gtgcccgggg ctctccatcc acgctcgcac aagatcttta
      541 tcatccttaa aattaccgag tgcgagattt tcaccgatga caatgtagtc gtagcccacc
      601 gcatccgcca aatcggctgc gcctttgcca tctggcgaat cgtgttcaaa atattgccgc
      661 gcaaacatat catccgcttt tttcgcagcg acacttgaga gcgttccgtc gagtgacaac
      721 gcggccacgc cgttcctgat gcgctcgttg ttcgtctccg caaaaatacc agaaactgta
      781 agcgtaccgt taaccgaccg gaaaatttta agcggttctg gattgacgag tttcttgggc
      841 agaatttctg tgacaaaaag ctccgcgtta tcccgagcca ctccgactgt atgttttaca
      901 tcctgaatta ctgaaggtct ccagacaaaa aaagtgctgg agagtgcaac aataatcgcg
      961 atcaaaaaat attttttcac ctctccatat tagacagaga atgccattct agcaaacttg
     1021 aagtcttctc aacttgagaa caatgacgaa gccctactct tcttcgtctg tatcctcaag
     1081 tatcggctca tccggaggag gtactactcc tgcatgtaat ccgtcagcgc acgcgcagac
     1141 ggagagattt tctccttctt tagagcagcc ttcgacgtcg caaactttag gagtctccga
     1201 ctcgccgccg caatcacctg aacaaacata atgtgtcata cgtttagaaa aaattattaa
     1261 agaaaatact atttatcatt cccgtcatgt cattttaaca attgatatta tatgaagagt
     1321 tggggtgtta ttaccactcg actgtccaca gaaatgcaac gtgacacatt ttaaaaaaaa
     1381 tgacaagtcc atgcgtacat ttaccccgtt agaaaccgcg atcgctgatc gcgatctgct
     1441 tttacgggat gaatgcaatt tgattttagc gagcatgagc tttattgccc cgtgagcttc
     1501 taacggggtt tatggcgtca cccgacattc aagcatcata aagagcggaa tctctttccg
     1561 cgctttgtct tcgattagtt tgcgtggccc agattcgctt ttcttatttg aaatccattc
     1621 ctcgaggcgt tcgacaacaa aaccagcctt gtgaagcgcc ttaaaatacg tctgaagcgg
     1681 ccgatgaaaa gaaatagtaa cagcgctgct ttgctgcccc ggatgtgctt taatttcagc
     1741 tttgctttcg ctggcatact cgtcgagccg acggtattga atttttagga cgtcgtcgaa
     1801 accccagctc gatttttttg ggattcgaaa tgcaggatgg tttaagacaa caatcaatct
     1861 cccgccatgt tttaaaacac gcgcacattc agccatcgtt tcatacagcg gcgcaatatt
     1921 ttgtatggcc agaattagcg ccgcaatgtc aaacgatccg tccttccatg gcaaatggtc
     1981 agcgcttgcc acttcatact ttacgcttcc tccttttttt tcggccagtg ttataagcgc
     2041 cttagaaata tcacacgcag caaccgatgc tccccccgtt gagaaggccc tcgagaaaaa
     2101 accttgcccg caggcaacgt cagcaatttg aagccctgtc cgaggctcaa taatacgcaa
     2161 aagatgtggc aaaatgactt ctgcctgaaa cgtctccgca gatccgagca gatcatcgta
     2221 ccaatccgaa accgcacccc atgaggtttc tttactttct tctttctgct tttcttttgc
     2281 ggccggcttt tttcgtatca tgagaaaata tttttcgaat gtttttccat tgagcccgcg
     2341 catcttttac gagcgcctga atttcggccg gatcgacatt cttcgccttt ttgtatttct
     2401 ttacaacttc ttcgacaatc cgattgtata cgtcttcatt gaccgcttca agcttctcga
     2461 gacgttcaat aatctcgcct tttgccttaa tgagccagcc acgaattttc tttctacgag
     2521 ccgctccatc tttatcgcca tataaaagat atgctccagc gagcgccgcg agtgtcgcaa
     2581 tacctgcgcc gactaggatt ttatttttct tctgtgccat ttccttttga atgtttactc
     2641 ttaacgaatc tccgactact aaagatcgct cggataaatc gtaaaatgga actcatttta
     2701 aatccggtag tccggatttc tgcgcacagc tctcttacaa gagtcgctat attgtttgtt
     2761 tccttgcgaa cttcggtaga tatgtgtttt gcatcgtgca atattagaat gacgtacaca
     2821 agagcaactc caccaagtac tccgatcgta atcaggacaa ccgtacttat gaagaaaaat
     2881 atgtcagcgt gaataatgct ttccatttca aacttatgat agcaacaact tttaaagctg
     2941 tctaagtaca gtttccatga ctacttgaac ctgatcggca tgacgagctc atcgtcgtgt
     3001 tcaggtagac cagatggatt atccttatgc aagacaagag ttcctgtcgc tgttgtgggc
     3061 acagttttaa aaacaagtgt tgcgcgaaaa ggtacaaaat tttcagtcat ccaatcacct
     3121 tgtgcctgtg ccggtgcaat tgcaatttgt ttgccgtttg aatccagaat ttctacggga
     3181 aatgacgctt cgaaaaacca atttcccctg gcttcgccgc caatagttag aggacttggc
     3241 acaattccac caagatgcgg tgacatgagc cgaatcagat cttgaccact tcgcgtcagc
     3301 ggtttttcta taaaagaagc ggtttcacta tcgcaaactc gagggtatga cagtaaaatc
     3361 gggaatcctg ccttaacaca atcttcaaaa gtattgactg tcacttgatt tatatcctcc
     3421 gtaaagcttt ttccatcctt tgtcttgcat tgccttggat acgactcgag tatagggttt
     3481 ccggcctcag cgcattcctc aaaatttgtc acatttttta ccatatatct gtctgttgga
     3541 cgcattttgt aatagagcaa taatgctaac gcaataagaa ttactatagg aattagaatt
     3601 gatatatttc ttctcatttt tttgttattt caaacgttat cttataattt ttttgatcaa
     3661 tctcccctcc tgtctctttt gcgggcagaa cgtcttttat acttactgta taagaacggc
     3721 tctcgattgt gaattctgca gtatctgttg atgtgacagt gagtgccgcg tgcgtacctc
     3781 ccttttcaac tgaaactt
//
LOCUS       DATDSU010000072         3612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1413719, whole genome shotgun sequence.
ACCESSION   DATDSU010000072 DATDSU010000000
VERSION     DATDSU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413719"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1240)
                     /locus_tag="VI981_02465"
     CDS             complement(185..1240)
                     /locus_tag="VI981_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4479193.1"
                     /translation="MSIVTQKINTRPRFTRHLVARGELRNSPLCVVDVGARGGFEKHW
                     SIYKNQVDLIGFELDREECIRLNKLEKNTHRTYYPIALHEKKGKKKFYIQPHLASSSF
                     YKSDKRFLERFPASKGMIPTKTVMIPTVGLDSFIKEHSIKGLDFIKLDVEGAELDIFK
                     GATGALKNSILGLSAEAVFFPWRHNIPTFTDIDIFLKPYGFVLFDLPIMKWERRSLSP
                     YMFEDKGVFGPTDRGQPVWTQAIYLKDGVNEMRSSKAKRKWWNRNRILKMASIMELFN
                     LEDCAIELIEEALAMEIVGAAEARSMIDLLTPRLNGRDVNYKKYVKYLKLEGPPRYID
                     GKRVSQKEFEKYKSLKH"
     gene            complement(1262..2347)
                     /locus_tag="VI981_02470"
     CDS             complement(1262..2347)
                     /locus_tag="VI981_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479194.1"
                     /translation="MGWEQSGCLALPLRNMKKTVYLLDHRFIPTPYNQRFLIEKIASG
                     FVRHGFDVKVIKKIDEIVNPGFVLVSHHDVFYNSIWGIRLLRADITRFLRPAKLLRSR
                     KVFSGVAYSDGVLGIVAEWMQALVLKKLSKKKDIIVIHWISCLQYWLEKFSMPYLASG
                     EHYRIEIPKNSYLWEWYNFYKTDRRAIPYPLSASVFPGDVGKDCQNEQYDVCYVGNKN
                     YQPDYQALFIGNPRARIVGTPPYIAEEERMRVYKNSKIALGLSYWESIATGMVTDRIV
                     EALAYGAICLSNNPYAPGATDGCAIYVRNKEELLERFNYFVNNTNERHALREKGFAFI
                     REKGTSYHRAAEFIERAKELYQPKFLQ"
     gene            2422..>3612
                     /locus_tag="VI981_02475"
     CDS             2422..>3612
                     /locus_tag="VI981_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY4479195.1"
                     /translation="MKRDVDICIVGGCGHVGLPLGLAFANKGKTVVLEDINEESVKMV
                     NAGRMPFMEHGAKPILTKSLKSGRIRATTDPTVIKSASNVIVVIGTPIDERLDPRTSD
                     VYKAIREIESYLSGEQLLILSSTLYPGTTQKIYQYLRKADKKTLVAFCPERIAQGHAI
                     KELYELPQIVSGCTGKAVVLATSLFKVLTKKIVTMEPTEAELAKLFTNAWRYINFATS
                     NQFYMLAQSNGLDFYKIYNAVTKDYPRMQSFAKAGLTAGPCLFKDTMQLSAFSGNNFF
                     LGHSAMLVNEGLPDFIVKTLQKNHNLTGMKVGLLGMAFKGDSDDPRTSLAFKLKKKLE
                     LESAIVLCHDPYIKDPSFVSLDTIRKKADIIILSAPHSIYKNMRWKKEVIDVWGFYKR
                     RSLLR"
BASE COUNT         1004 a          844 c          765 g          999 t
ORIGIN      
        1 tgagctcgca cttcgtcaac agatccgtcc tcgtactcaa gctcgcgaac atctttataa
       61 acatctgctt ttacttcaat aacagagtgc tcgcttggtg ggaaatcaac gttaatgtac
      121 ccttcgaggt atttctcccc acaacctagg tgcaatttta ctttcttttc ttcgttcaca
      181 agatttagtg ctttaaagat ttgtattttt caaactcttt ttgcgacacc ctcttcccat
      241 cgatatatct cggtggaccc tcgagtttaa ggtatttgac atatttctta tagtttacat
      301 cacgaccatt gagccgcggc gttaacaaat caatcataga acgggcttca gccgctccaa
      361 ctatctccat cgctagagct tcctcaatta gttcaatggc gcagtcctca aggttaaaaa
      421 gctccatgat actcgccatt ttaagaatcc ggtttctgtt ccaccacttc cttttagcct
      481 ttgaggagcg catttcgtta acaccatcct tgagataaat cgcctgtgtc cacaccggct
      541 ggccgcgatc tgtgggaccg aaaactcctt tatcttcaaa catatatgga gatagtgacc
      601 tgcgctccca tttcataatc ggcaggtcga acaatacaaa gccgtagggt tttaagaaga
      661 tatcgatgtc cgtaaaagtc gggatattgt ggcgccaagg gaaaaatacc gcctctgcgc
      721 tcaagcccag aatcgaattt ttgagtgccc cagtagctcc cttgaaaatg tctaattccg
      781 ccccctcaac gtcgagtttt ataaaatcca gcccttttat agagtgttcc ttaatgaatg
      841 aatctagtcc taccgtagga atcataaccg ttttcgtcgg tatcatgccc ttagacgccg
      901 gaaaccgttc taggaaccgc ttatcggatt tataaaaact agacgaagcg aggtgcggtt
      961 gaatataaaa tttcttcttc cccttctttt cgtggagagc tataggatag taggtcctgt
     1021 gggtgttttt ctcaagttta ttcaaccgga tacactcttc acggtcaagc tcaaagccga
     1081 ttaaatcaac ctgattttta tagatactcc agtgtttttc aaatccgccc ctagcaccga
     1141 cgtctacgac acaaagcggc gagtttctaa gctcgcctcg tgcgactaaa tgacgagtaa
     1201 acctaggccg cgtattaatt ttttgagtaa cgatagacac gaaatctttg ctatttgtat
     1261 tctactgcaa aaatttcggt tgatatagct ccttcgcgcg ctcaataaat tctgcggccc
     1321 gatgatagct cgtgcctttt tcgcggataa acgcaaaacc tttttcgcgc agagcgtgac
     1381 gttcgttggt attattcaca aagtaattaa atcgctctaa taattcttcc ttgtttctta
     1441 catagatagc gcagccatca gttgcgccag gtgcgtacgg gttatttgat aagcagattg
     1501 ccccataggc aagcgcttca actatgcggt ctgtgaccat cccagtggcg atggattccc
     1561 agtagcttag cccaagcgca attttggaat tcttataaac acgcattcgt tcctcttctg
     1621 ctatatacgg aggcgttcct acgattctcg ctcgcgggtt accgataaac aaggcctgat
     1681 agtccggttg gtaattcttg ttgccaacat aacaaacgtc gtattgctca ttttggcaat
     1741 ctttaccgac gtcacctgga aaaacactgg ccgaaagcgg atatggaatt gcgcgcctgt
     1801 cggttttata aaagttgtac cattcccaaa gatatgaatt ttttggaatt tcgattcggt
     1861 aatgctcacc agaggcgaga tacggcatgc taaacttctc gagccagtac tgcaaacacg
     1921 aaatccagtg aatcacgatg atgtcctttt ttttacttaa tttcttgagt acaagagcct
     1981 gcatccactc agcaacgata cccaaaacgc catccgagta agcgactccg gaaaaaactt
     2041 tgcggcttct taacaacttg gctggacgta agaagcgggt aatatcggct cgcaacagac
     2101 gaattcccca gatcgagtta taaaaaacat cgtgatggga gacgagcaca aacccaggat
     2161 ttacaatctc atcaattttt ttaatgactt tcacatcaaa tccatgccta acaaaccccg
     2221 atgcgatttt ttcgatcaag aaacgttggt tatatggtgt cgggataaaa cggtggtcga
     2281 gtagataaac agtttttttc atattccgca acgggagcgc caaacagcca gactgttccc
     2341 agcccatagc ttaacttatt tttaaagaaa aaccagcttg tcttgctcgg gacattcgtg
     2401 gttttgtata cttagtattc catgaaaagg gatgttgata tttgcatagt gggtggctgt
     2461 ggccatgtag gattgcccct aggcctcgcg tttgcgaata aagggaaaac agtagtgtta
     2521 gaagatatta acgaggagag cgtaaagatg gttaacgcag gacgcatgcc atttatggaa
     2581 cacggagcaa aacccatact tacaaaatcc ttgaagagcg gacggatccg cgccaccaca
     2641 gatccaacag ttataaaaag tgcgtcgaac gtgattgttg tcatcggcac accgattgat
     2701 gagcgtctag atccgcgtac atccgatgtt tacaaagcaa tacgcgagat agaatcgtat
     2761 ttgtcgggcg agcaattgct tatcttaagt agcacgctct atccaggtac gacacaaaag
     2821 atttaccagt atttaaggaa agcagataag aaaacgcttg tcgctttttg cccagaacgc
     2881 atagctcagg gtcacgcaat taaagaactt tacgaactac cgcagattgt ctctggttgc
     2941 acaggaaagg ctgtcgtgct cgcaacttca ctttttaagg tgctgacgaa aaaaatagtc
     3001 accatggagc cgacagaggc ggagttggca aagcttttta caaatgcgtg gcgctatatt
     3061 aattttgcca cctcaaatca gttctacatg ctcgcccaga gtaacggctt ggacttttat
     3121 aagatttaca acgcggtgac taaagactac ccgcgcatgc aatcgttcgc aaaggcaggt
     3181 ctgactgccg gaccgtgctt gtttaaggac acgatgcaac tttcagcgtt cagtggtaat
     3241 aattttttcc tggggcattc ggccatgcta gtaaacgagg gattgcctga tttcatcgta
     3301 aaaactctgc aaaaaaatca caatctcacg ggcatgaagg taggtttatt gggaatggcc
     3361 ttcaagggag acagcgacga cccgcgcact agtcttgcat tcaaacttaa aaagaaattg
     3421 gagctcgagt cggctatcgt actctgccac gacccctaca ttaaagatcc aagctttgtt
     3481 tctctagaca ccattcgaaa aaaagctgac atcatcatct tgagcgcgcc gcacagcatc
     3541 tacaaaaata tgcgatggaa gaaagaagtg atcgacgttt ggggtttcta caaacgtcga
     3601 tcacttctta ga
//
LOCUS       DATDSU010000073         7566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2077809, whole genome shotgun sequence.
ACCESSION   DATDSU010000073 DATDSU010000000
VERSION     DATDSU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077809"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VI981_02480"
     CDS             complement(<1..1262)
                     /locus_tag="VI981_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEY4479196.1"
                     /translation="MKTILITSFHPLISRNILFSPVLDLLRASGVRVVVIGRTKKKEF
                     FKKELPCVEVVSLERKLNAGDRFLRYLSLAGLPTDSIKLKQKTEMKGSGRFLSLWFRG
                     WFARQSVRLIFKILIRGTRDFISILDEVHPDLIFSTDIQHPSDLGLFAAARRKKISIA
                     GMVRSWDNPTSKGLIGVVPDILLVHNRLVKSEVEALHKILPSKIRVVGVPHYDEYDPM
                     HAEKTESFYKKTGLDPKRPFLLFTPTGDRYISNNTVDRDILDMLDKILPSEYQILVRF
                     PFTDTVAYLDNKKNYGRIFFDRPEMNFELHKNNELSKEGEKHLRLTLALCSLVVTGPS
                     TICIDAAIFDKPIILIAFDGREKRKPLESIARYYMYDHWKPIHASEGASFAGSEQELA
                     TLIERYIRNPALHSGGRKKIVETELEFT"
     gene            complement(1283..2662)
                     /locus_tag="VI981_02485"
     CDS             complement(1283..2662)
                     /locus_tag="VI981_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEY4479197.1"
                     /translation="MSKTIVITSFHTLISRNILQTPIIGLLQKSGFDIVIAVPNFKVP
                     YFEKTFKRLGVTIEGFEIGAPTRTKRVGMLKRLAEALPDSRRAELGRKLTLSGQTKSK
                     FYYYLFYYPAHFLGKSFFLMRLLRVLDYVLSPRGRFYKLFEDHKPILVFSTDIQNEHD
                     VAIMQDAKSRKIPVLGMVRSWDNVVTRAFRFVPKRILIHNAIIGEQANSLYGIDKKRF
                     VVTGVPHYDAYLENSKPDRNQKFKEWQLASLKKTVLFFPLCDYRVVRESNEKQIYMDK
                     LILETLQAFDCNVIVRFPPNETVSIPGFDKPANFFYDRPGVGFDAAGVTTREIRKEDD
                     ESLRAELSLADAVVCGPSTAIIDAALFDKPIVCIDFEIGKKDSLHGFIFEYQSEHIVS
                     VLRTKGIARVKTVEELKTALTAYFKDPSRDQAGRRQIVNEQCYKTDRLSSKRVALEIL
                     EFLTPNPKP"
     gene            2846..4879
                     /locus_tag="VI981_02490"
     CDS             2846..4879
                     /locus_tag="VI981_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEY4479198.1"
                     /translation="MNAKKIWRGLANLKNTEIRRTYVRRFRTRVQTKKRQLFDAHPRV
                     IRAGKAIFGDKLPVFIIAEIGPNHNGSLERALELIDGAHKAGAQAVKFQKRFLEATYR
                     KDVVEHPEMNDQAFQYLIPILKETEFGEAEYKKIKTHCDKLGIFFIATPFDEESVDFL
                     ERIINPPFYKVSSADLVNIPLLEKLLTTKKPLILSTGMSTPDEISEVVGFLHQKKAIF
                     ALMHCQSTYPAAPETLNLAFIKQLKARFGVPVGYSGHEVGIDTTLIAVALGACIVERH
                     ITFDQKALGPDHAASLTPPLFKELVERIRETEQAIGLGGRQLTRGEVMNRLSLRKSLV
                     ATVAIKTGQKIERSMVRAKSPGTALSPLRLYDVVGRIARRDIEADNFIHESDVYEKDT
                     LQKIPHFKTSWGLKARFFEIKDIRKFTPQPSFLEFHVTDKDLDYTFGKDETYPYELYI
                     HAPEYNGKLPVDLASDDDLLWEKSIEIIQRTIDKTRDIAKHFKGTPKIVIHVGGMSIK
                     PNLERKKMLERAIAAFKRLEWEGVEVMPENLPAFGWFFSGLWHINFFGAAEDMIEFCK
                     RLNLPMCLDLSHAWLYCQAAKKDYLAFIEAVAPYVAHLHISDGRGSHKEGLQIGEGDV
                     PFRETLKILEQGFKKSGRKLSWVPEIWQGHLNNYQEFKIALARLAKYPFLGGE"
     gene            complement(4880..6670)
                     /locus_tag="VI981_02495"
     CDS             complement(4880..6670)
                     /locus_tag="VI981_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4479199.1"
                     /translation="MSENVLGIKMIGHDTGAAVISNGRVIAISEERLNRIKHSLNMFP
                     HKSIAYCLEALSLKPEDIHLILIDRIRAPEERGTPEELFKARSIVAFPKAKIVTVNHQ
                     EAHAASTFFASPFEDAGILVYDGFGEDFISHTGVKVVESDTLFFGKGNHFTQIAKTAH
                     RVKGRDFPYTTGIGMLYSKISQKYIRLGKFNEGKMMGLASYGNDSILKMFPPERWFRD
                     YMGSIVVNARIAFARPNFRQLMRNAKSWRARIRFFYYWLTGFASIPWSVFRRWRSKNE
                     LFPELRLPRPRRHPKIDKLPDDYYASVAYAVQKVLEIVCLSWAKKLFAVTQSQNIAIA
                     GGVGLNIDANKKILDESGFKHIFIQPGATDCGIPLGLALYGLHMVLGEPRFFKMQGAA
                     LGRPYTETEIQKALSRFSDKITYKKSATSVHDAAAEIARGKIIGWFTGGSEYGPRALG
                     HRSILCDARNKDMKDIVNARVKHREAWRPFAASVLLEDMGRYFDLKEESPFMILCADV
                     HKNMRTKIPSVVHVDGTCRIQTVTRESNARYHELLSEFKKLTGESIVLNTSFNLGGDP
                     IVETPEDALDSFLRTDIDCLVIEDYIIRKK"
     gene            6751..7512
                     /locus_tag="VI981_02500"
     CDS             6751..7512
                     /locus_tag="VI981_02500"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEY4479200.1"
                     /translation="MKKRGKIYAIIPARGGSKSIPRKNILSFGGYPLVAFSIAAAKLA
                     KKIDRIIVSTDDEKIARVAKKFGAEVPFMRPKEISGDLSTDREFFVHAIKWFEKAESE
                     IPELMVHLRPTSPLRDPKHIDQAIDMIHKDAKATSLRSAYEVAARPQKFFLRENGYFV
                     PLIQEDKKYEYHLTPRQMFPAAYNPDGYLDIVRSSRIFKNEDSLHGNKILAFVSHNTG
                     DIDHTNDLAYAEKLIADKEWLVYEYLKRHFPQDTK"
BASE COUNT         2116 a         1742 c         1697 g         2011 t
ORIGIN      
        1 tctgtgaatt caagttccgt ctccacaatt tttttccttc ctccgctgtg cagtgccgga
       61 ttcctaatgt aacgttcgat aagtgtcgca agttcctgct ccgaaccggc aaacgacgct
      121 ccttccgaag catgtatcgg cttccaatgg tcatacatat agtagcgcgc aatgctttca
      181 agcggcttgc gtttctccct tccgtcaaaa gcaatgagaa taataggctt atcgaaaatg
      241 gccgcatcaa tgcaaatcgt cgaaggaccc gtgacgacta gtgagcataa tgccaacgtg
      301 agacgtaaat gtttttcacc ctcttttgaa agttcattgt tcttatgaag ttcaaaattc
      361 atttccggcc ggtcaaaaaa tatccgtccg tagttttttt tgttatccag atacgcgaca
      421 gtgtccgtaa atggaaaacg gacaagaatc tgatattcac tcggaagtat tttgtccaac
      481 atatctagaa tatcccggtc aaccgtatta ttgctgatat accggtcccc cgtcggcgta
      541 aaaagcagga acgggcgctt gggatcgaga cccgtttttt tgtagaaaga ctctgttttt
      601 tcggcatgca taggatcata ttcgtcataa tgcgggacac ccacaacgcg gattttagat
      661 ggtaatattt tatgaagcgc ttcaacttcg cttttgacga gtctgttatg aactaaaaga
      721 atatccggca caactccgat caatcctttt gatgtcgggt tgtcccatga gcgaaccatg
      781 ccggcgatgg aaattttctt tcttcgagcg gcggcgaaaa gcccaagatc agacgggtgt
      841 tgaatatcgg ttgaaaaaat aaggtccggg tgtacttcat ctaagattga aatgaagtct
      901 cgggttccac gaatgagaat tttgaaaatt aatcggacac tttgccgcgc aaaccaccca
      961 cgaaaccaga gcgacaaaaa gcggccgctt cctttcattt cggttttttg cttcagttta
     1021 atagagtcag tcggaagacc tgcgagcgaa agatatctca aaaaccgatc acccgcgttt
     1081 aattttcgtt cgagggaaac gacttcgaca cagggcaact cttttttaaa aaattctttt
     1141 tttttagtgc gtccgattac caccactcgt acccccgacg ctcgaagtag atcgagaacg
     1201 ggactaaata gaatgtttct tgagatgagt ggatggaagc tggtgataag aattgtcttc
     1261 ataaaaagaa agtgagtagg gtttagggtt tggggtttgg ggttaaaaac tcgaggattt
     1321 cgagggcgac gcgcttcgac gagagtctat ctgtcttgta acactgctcg tttacgatct
     1381 gtctcctgcc cgcctgatct cggctcggat ctttaaagta cgcagtaagg gcagttttca
     1441 attcttctac tgttttcacg cgggctattc ccttagtgcg aagtactgac acgatgtgtt
     1501 ctgactggta ttcaaagatg aagccgtgaa gactgtcttt ttttccaatc tcaaaatcta
     1561 tgcagacgat tggtttatcg aagagcgctg cgtctatgat ggccgtagac ggcccgcaga
     1621 caacggcgtc ggcaagagaa agttccgcgc ggagcgattc atcatcctcc tttcgtattt
     1681 ctcttgttgt cacgcctgcc gcatcaaaac caacgccagg ccggtcgtaa aagaagttag
     1741 cgggcttatc gaatccggga atcgatacgg tttcattcgg tggaaaacgg acaatgacgt
     1801 tacaatcaaa tgcctgaagt gtttcaagaa taagtttatc catatatatc tgtttttcat
     1861 tggattcgcg caccacccga taatcgcaga gtggaaagaa gagcacggtt tttttcaaag
     1921 aagcaagttg ccactccttg aacttctggt tccggtctgg ctttgaattt tcgaggtatg
     1981 catcgtagtg aggtacaccg gttaccacga accttttttt atcaattcca tagagtgagt
     2041 tggcctgttc cccaataatt gcattgtgaa tcaagattct cttcggcaca aagcgaaacg
     2101 ctcgcgtgac tacgttatcc catgatcgca ccatgccaag cacaggaatt tttctcgact
     2161 ttgcatcctg cataattgcc acgtcgtgtt cattctgaat atctgtgctg aaaacgagaa
     2221 tcggtttatg gtcctcgaaa agtttataga agcgaccccg aggagacaaa acataatcaa
     2281 gtactctaag cagtcgcatc agaaaaaatg atttaccgag aaaatgcgcc ggataataaa
     2341 aaagataata gtagaatttg cttttcgtct ggccgctaag cgtcaatttc ctcccgagct
     2401 ccgctcttcg agaatctgga agagcttcag cgagacgttt cagcatccca acccgtttag
     2461 ttctggtcgg cgctcctatt tcaaagcctt cgatcgttac accaagccgc ttgaatgttt
     2521 tttcaaaata tggaacttta aagtttggta ctgcaatcac aatatcaaat ccgctttttt
     2581 ggagcagacc gataatcggc gtctgcaaaa tattgcggga aatcagcgtg tggaagctcg
     2641 tgataacgat tgtttttgac attcgaatgg agatatgcga acttttgtag tctatacgaa
     2701 tttgacacat ttccgcaagg aggaatgccg aaacatccgt gtgtcagtct gtcaacccta
     2761 cattaatttt acgcggccgc acaaaattaa tgtatgttcg aaacattcgt gtcgaaattc
     2821 aagttatggt atatgatttg tgaacatgaa tgcaaagaaa atctggcgag gactggcaaa
     2881 ccttaaaaat acggagataa gacggaccta tgtccgccgt tttcgaacgc gtgtccaaac
     2941 taaaaaaaga cagttattcg acgcacaccc tcgagtcatt cgcgcgggca aagctatttt
     3001 tggcgataaa ttgccggtat ttattatcgc cgaaatcggg ccaaatcata acggttctct
     3061 tgagcgagcg ctcgaactta tcgacggagc gcataaagcc ggagcgcagg ctgtcaaatt
     3121 ccaaaaacgt tttcttgaag caacctatcg aaaagatgtt gttgagcatc ccgagatgaa
     3181 cgatcaagcg tttcaatatc tgattcctat tttaaaggaa accgaattcg gcgaagcaga
     3241 gtacaaaaaa ataaagacgc attgcgataa actcggaatt ttttttattg ccacgccgtt
     3301 cgatgaagaa agcgttgact tcctcgagcg cataatcaat cccccatttt ataaagtatc
     3361 ttctgcagat ctcgtcaata ttccacttct tgagaaattg ctcaccacaa aaaaaccgct
     3421 catactttca acaggcatgt cgacaccgga cgagataagc gaagtggtcg gtttcttaca
     3481 tcaaaagaaa gcaattttcg cgcttatgca ctgtcagagc acatatcctg cggcgccgga
     3541 gacactcaac ctcgctttca taaaacagct aaaagcgcgc tttggcgttc ctgtgggcta
     3601 ttctggccac gaagtcggaa tagacacgac actcattgcg gttgcgcttg gcgcgtgcat
     3661 cgtcgagcgt catattacct ttgaccaaaa agctcttgga ccggaccatg ccgcttctct
     3721 cactcctcct ttatttaaag aattggttga aagaattaga gaaacggagc aagccatagg
     3781 actcggaggc cgccagctca ctcgtggtga agttatgaac cgcctctcgc tccgaaaaag
     3841 tcttgtggca accgtcgcca ttaaaacagg gcaaaaaata gagcggagta tggttcgggc
     3901 aaaaagtccg ggaaccgcgc tctccccgct tcggctgtac gacgtcgtcg gcagaattgc
     3961 gcgacgcgat attgaagcgg ataattttat acacgagagc gatgtgtatg aaaaagatac
     4021 tcttcagaaa attccgcact ttaaaacatc atggggtttg aaggcacgct ttttcgaaat
     4081 aaaagatata agaaaattca cgccgcaacc gtcatttcta gaatttcatg tcacggacaa
     4141 agacctcgac tatacattcg gcaaagacga aacttatccg tatgaactct acatccacgc
     4201 gcctgaatac aacggcaaac tgccggtcga tctcgcctcc gacgacgacc tgctctggga
     4261 aaaatcgatt gaaatcattc aacgcaccat cgacaaaacc cgcgacatcg ccaaacattt
     4321 caaaggcaca ccaaaaattg tcatccacgt tggcggcatg agtataaagc cgaatttaga
     4381 gcgcaagaaa atgctcgagc gtgccatagc tgcgttcaaa cgtctcgaat gggaaggcgt
     4441 cgaagtgatg ccggagaacc ttcccgcttt tggctggttt ttttccggac tttggcacat
     4501 caatttcttc ggtgcagcag aagacatgat tgaattctgc aagcgactta atcttcctat
     4561 gtgcctcgac ctttcacacg catggctcta ctgccaggct gcaaaaaaag attatctcgc
     4621 ttttatagaa gctgtcgcgc cctacgtcgc tcatctccat atttctgacg gccgaggctc
     4681 tcacaaagaa ggactgcaaa ttggggaagg cgatgttccc ttccgagaaa ccttaaaaat
     4741 tctcgagcaa ggcttcaaaa aaagcggccg taaactctcc tgggttcctg aaatctggca
     4801 aggccatctt aataattatc aagaattcaa aatagccctt gcgcggcttg cgaaataccc
     4861 ttttcttggc ggagagtagt tatttttttc gaatgatgta atcctcgatg actagacagt
     4921 cgatatcagt acgaaggaat gaatcaaggg catcttctgg agtctcaaca atcgggtcac
     4981 ctccaaggtt gaatgaggta ttcagcacta tagattctcc ggtcaacttc ttgaactccg
     5041 agagaagctc atgatatcgg gcattcgatt ctctggtcac tgtctgaatg cggcaagttc
     5101 catcaacgtg aacaactgac gggattttgg tccgcatatt tttgtggaca tcggcacaaa
     5161 gaatcataaa cggactctct tccttgaggt caaaatagcg ccccatgtct tcaagcaaca
     5221 ctgaagcggc aaaaggtctc cacgcttcgc gatgtttcac cctggcgtta acaatatctt
     5281 tcatatcttt atttcgtgca tcgcaaagga tggaacgatg gccaagcgcc cgaggaccgt
     5341 attcagaacc tcccgtaaac cagcctatga ttttcccgcg agctatttct gcggcagcgt
     5401 cgtgaacgga ggtggcagat ttcttgtaag ttattttgtc actaaaacgc gagagtgcct
     5461 tttgtatttc agtttcagta tacggccggc cgagtgcggc tccctgcatt ttgaagaatc
     5521 gcggctcgcc gagaaccata tgaagtccat agagcgcaag tccgagcgga attccgcaat
     5581 ctgtggctcc aggctgaata aaaatatgct tgaaacccga ttcgtcaaga atttttttgt
     5641 tggcatcgat gtttaatccc acgccgccgg caattgcaat gttttgagac tgggtcactg
     5701 caaaaagttt cttagcccac gagagacaca cgatctcgag tactttctgc acggcatatg
     5761 caactgacgc atagtagtcg tcgggcagtt tgtcgatctt tggatgccgg cgtggacgag
     5821 gaagtcgtag ttcaggaaaa agttcgtttt tcgatcgcca ccttctaaac acactccacg
     5881 gaattgacgc aaaccctgta agccaatagt agaaaaaacg gattcgggcg cgccatgatt
     5941 tcgcgttgcg cattagttgc cgaaaattcg gccgagcaaa ggcaatccgc gcattgacta
     6001 cgatagatcc catataatct ctaaaccagc gttcgggtgg aaacattttc aaaattgaat
     6061 cgttgccgta cgacgcaagg cccatcattt ttccttcatt aaatttgcca agtcgaatgt
     6121 atttttgcga aatttttgaa tagagcattc cgattcctgt cgtatacgga aaatctctgc
     6181 ctttcactcg gtgagcagtt tttgcaatct gggtaaagtg attgcctttg ccaaaaaaaa
     6241 gcgtatcgga ttccacgact ttcacgcctg tgtggcttat aaaatcttcg ccgaatccgt
     6301 cgtacactaa aattccggca tcctcgaaag gagaggcgaa aaatgtcgaa gcagcatggg
     6361 cctcctgatg attgacggtg acaattttcg ccttcggaaa agcgacaatg cttctcgctt
     6421 taaataattc ctcgggcgtt ccgcgctctt cgggagcgcg gatacgatca attagaataa
     6481 ggtgtatatc ttcgggttta agtgaaagag cctctaggca ataggcgatg gatttatgag
     6541 gaaacatatt gagtgaatgt ttgatacggt tcaaccgttc ttcggatatt gcgatgactc
     6601 ttccgtttga aataacggct gcacccgtat catggcctat catttttatg ccgagtacgt
     6661 tttctgacat gtgagaagta taacaaagac agccagtgca acaattttga ctagtggcag
     6721 gttatcattg tgttgttgta tgatacgcat atgaaaaaac gaggaaaaat atatgccatt
     6781 attccggcgc gcggaggatc aaaaagcatt cctcgaaaga atatcctttc tttcggagga
     6841 tatcctctgg ttgccttttc gattgcggcg gcaaaactag caaaaaaaat tgaccgaatt
     6901 atcgtttcaa ctgacgatga gaaaatcgca cgggtggcaa aaaagtttgg agctgaagtt
     6961 ccattcatga gaccgaagga aatttcaggt gatctctcaa ctgatcggga gttttttgtc
     7021 cacgctatca agtggttcga aaaggcagaa agcgaaatac cggaacttat ggtccacttg
     7081 aggcccactt ccccacttcg agatccaaaa catattgatc aagcgataga catgattcac
     7141 aaagacgcaa aggccacatc gcttcggtct gcatatgagg tagcggccag accacagaaa
     7201 tttttcctgc gggagaacgg ttattttgtg ccactcatac aagaagacaa aaaatatgaa
     7261 taccacctta cgccgcggca aatgtttcct gccgcgtata atccagacgg atatcttgat
     7321 atcgtacgga gctctcgtat tttcaagaat gaagattcgc ttcacggcaa taagattcta
     7381 gcgttcgtca gccataatac gggtgatata gatcatacaa acgacctcgc atacgcggag
     7441 aagctaatcg ccgacaaaga gtggctcgta tacgaatacc ttaagagaca ttttcctcaa
     7501 gacacaaaat gacctgtccc gttagaaatc cgatacgcac aaatatttct agagcttttc
     7561 ctctac
//
LOCUS       DATDSU010000074         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2402346, whole genome shotgun sequence.
ACCESSION   DATDSU010000074 DATDSU010000000
VERSION     DATDSU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402346"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..306)
                     /locus_tag="VI981_02505"
     CDS             complement(109..306)
                     /locus_tag="VI981_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479201.1"
                     /translation="MLSKKLYTLLTGLIFTVVVLIHLARIWAEWAVEVGPYSVPTAAS
                     WIAVVLAGYLAYNGFRLHGKD"
     gene            452..1786
                     /locus_tag="VI981_02510"
     CDS             452..1786
                     /locus_tag="VI981_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY4479202.1"
                     /translation="MDIKIDPLPDEYKKYIGPLVVTVLSLLPVALWVVAKPIEDRFFS
                     IEATALSFGQIAGLVGMALFSVAMILSARLKIITRYFDGLNKEYSTHHTAGIISFILL
                     MAHPLILSFGYLRVSVSAMAQFLLPRNDLAINFGIIALIGLVVLILITLFAKFKYEVL
                     FTLHKFLGLVFVFASVHMLLAPGDLRSNPPLAAYMSLLMAAGIVAFFYRTLLGHFAVS
                     RFSYRVVGAREVGDDVLEIEMEPVGEKIPYRAGQFIFINYVSDTVNTEAHPFSLSSGA
                     EEANVKVAIKNLGDYTKGLFQLEKGSTAEIEGPFGRFFYEYESIRDKVWIAGGIGVTP
                     FLSMARTMRSKMENEYRVFFFYSVKKKSEFVFLDELIAISKVCKWFIFIPYCTDQSGF
                     LTAQIIDKMTRGVRKKDIYICGPLSMMKALGSQFIKLRVPKRHIHMEQFKLL"
BASE COUNT          545 a          407 c          464 g          620 t
ORIGIN      
        1 gactttttga ggagtactca gggtaaagaa atgatccctt tcgggagctc gttatctcgt
       61 aaaccaataa ttgatagcat atagggaaat gcgagacaag tctagttttt agtcttttcc
      121 gtgaagtcgg aaaccgttgt aggccagata gcccgcaagt acgactgcga tccaacttgc
      181 tgcggtgggc acggaatatg gtccgacttc gactgcccac tctgcccaaa ttctcgccaa
      241 atgaatcaag acgacaacgg tgaagataag gccggttagt agtgtgtata gttttttcga
      301 taacatatat ttgagtgatt aattgcttga attgtatctc ttaacgataa cttgtacaaa
      361 atattctggg gatagctcgt ctcgccctgg gtttagacaa atactgctgt tgatggggat
      421 gtaattcaat acggtataat gtgaggcatg tatggatata aaaattgacc cacttcccga
      481 cgaatacaaa aaatacatag gtccactggt agtgactgta ctctcgttac ttcctgttgc
      541 gctctgggtt gttgcgaagc caattgaaga ccgttttttc tctatcgaag ctaccgctct
      601 aagtttcggg caaatagcgg gccttgttgg gatggctctt ttttctgttg ccatgatctt
      661 aagtgcgcgg cttaaaatca taacgcgtta ttttgacggg ttaaacaagg aatatagcac
      721 ccaccatacc gctggaatta tttccttcat tctccttatg gcccaccccc ttattctttc
      781 ttttggttat ctccgtgtct ctgtttcggc catggcacag ttcttgcttc cccgcaatga
      841 tcttgccatt aatttcggga ttatcgcgct tattggatta gtggttctca tattgattac
      901 tctttttgcc aaattcaaat acgaagtgct tttcactctc cataaatttc tcgggcttgt
      961 ttttgttttt gcttcggtgc acatgttact ggctccaggc gacttgagaa gtaacccgcc
     1021 gctcgcggcg tacatgtctc tgcttatggc cgccggaatt gtcgcttttt tttaccgaac
     1081 attgttgggg cactttgcag tcagtcgatt ctcgtatcgg gtcgtaggag ctcgtgaagt
     1141 aggcgatgac gttctcgaga tcgaaatgga accagtcggt gagaaaatac cgtatcgggc
     1201 aggtcagttt atattcataa attacgtgag tgatactgtg aacactgaag ctcatccatt
     1261 ctcactttcg tccggtgccg aggaagccaa cgtaaaggtc gcgatcaaaa atctcggtga
     1321 ctatacaaaa gggttatttc agcttgaaaa gggttcgacg gccgaaatcg agggaccttt
     1381 tgggcgtttt ttttatgaat atgaaagcat tcgagataaa gtatggattg ccggagggat
     1441 tggggtgacg ccctttttga gtatggcgag aacaatgcga tcaaagatgg aaaacgaata
     1501 tcgggttttt ttcttctatt cagtcaagaa aaagagcgaa tttgtatttt tggacgaact
     1561 tatagctatt tcaaaggttt gcaagtggtt tatttttatc ccctattgta ctgatcaatc
     1621 ggggttcctg actgcgcaaa ttattgataa aatgacacgt ggggttcgca agaaagacat
     1681 atacatatgc gggccgcttt ctatgatgaa ggcgctcggc agtcagttta ttaagcttcg
     1741 tgtgccaaag cgacatattc acatggaaca gtttaaacta ctgtagactt aacggtatga
     1801 atggggtagt agaaaatgga atataaaatt aaagcaaaag taaaccaaac attatcgaca
     1861 caaagtgtaa aaaactcatt tagtgtgtcg atgctatttt tcactacccc atgaatcccg
     1921 ttagaaatcc aagtattatg tatttactac ctatctcaat aaaatcaagt agtcgaggta
     1981 ctgagacgct tatactacaa atggcgggtg cgccttcacg aaaaacgtct gacagc
//
LOCUS       DATDSU010000075         2503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_231485, whole genome shotgun sequence.
ACCESSION   DATDSU010000075 DATDSU010000000
VERSION     DATDSU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231485"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..929)
                     /gene="tpiA"
                     /locus_tag="VI981_02515"
     CDS             complement(150..929)
                     /gene="tpiA"
                     /locus_tag="VI981_02515"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY4479203.1"
                     /translation="MKNRNGRKYIVANWKMNPDSLWEAKFFFTEIKKIARVLPRVTTV
                     VCPPAVYLEALTGSYNQNRITLGAQDVSTEERGSFTGEVSAAMFQKIGAKYVIVGHSE
                     RRGRGETDSEVQKKVLACLRHNLAPILCIGEKQRDADGTHLAFIKNELKEDLAKVTYK
                     ELQKIVIAYEPIWAIGKKDTEAMSPPQIEEMLIFIRKELTDLYRGASMKNVRILYGGS
                     VSVRNAESMLENSSIDGFLIGNQSLKPDGFCQILKYAHASR"
     gene            complement(1007..>2503)
                     /locus_tag="VI981_02520"
     CDS             complement(1007..>2503)
                     /locus_tag="VI981_02520"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HEY4479204.1"
                     /translation="EVEVVDIVGGKQLISTATCGSFVRKAKAIMTTGIGAIFNYGIQT
                     GQGGFIMGNATVNGSVYSGGNITGSNNATITGAAVASNPPTASPDQTNITPTPPTNSI
                     NFADGASTQDFAQSFVLSSSGKNSLVQLYIKKVGDPSSATVRIVSNSGGSPGTASLTT
                     GTLNSNFVTTTYGLVDIVFSSNVNLTSGITYWIVVDSPTDSASNYYTIGANSSYTSGL
                     ANIGAFGGSWGDTSPAGLDGYFTIYLEGAPAVIDNIDIGTAGVGDAWAHAVNNSKVNG
                     SLYCQTGSGNTDENSNALSCSVAVDPVSQNMPVSESNIEQWKNDAVTDGEVTCSGGNY
                     TPATGASLGPKKIPCNLVIDNNFTLTGILWVTGNVTTSNGRTVTLASSFGTRSSVIVV
                     EGTTAISNNSLFNGTGQTGSYIMILSTNTGTSPAAIDVGNNAGTVILNAQKGTIHFNN
                     NSGAKEATARLIDMDNGSTVTYDSGLVNTIFSSGPSGGYVFGSWKEVE"
BASE COUNT          674 a          589 c          511 g          729 t
ORIGIN      
        1 ggacgttgtg caaactaaga agtttgcacc cgctcctcgc tagccaaaat aaagttcatg
       61 agacagagcg tttccgtagt taatctaaca atttctatct gtatattatt gtatatcaac
      121 atcgtgatat atacacaaca ataggatctc taacgggaag catgcgcgta ttttaatatc
      181 tgacaaaaac cgtcgggttt caagctttga tttccgatca gaaaaccgtc tatcgacgaa
      241 ttttcgagca ttgattcggc attccgtaca ctgactgaac cgccgtataa aatccgaacg
      301 ttcttcattg atgccccccg ataaaggtca gtcaactctt ttcgaataaa aataagcatt
      361 tcttcgatct gcgggggaga cattgcctct gtatctttct tgccgattgc ccaaataggc
      421 tcataggcga tgactatttt ctgcaattcc ttataggtaa ctttcgcaag atcctcttta
      481 agttcattct tgatgaacgc caagtgtgtt ccgtcggcgt ctctttgttt ttctccaatg
      541 cacaagatgg gcgccagatt gtgtcgtagg catgccagta cttttttttg aacctcggag
      601 tccgtttctc cacggccgcg ccgctcagaa tggccaacga ttacatattt tgcgccgatt
      661 ttttgaaaca ttgcagcaga aacttctcct gtgaaagaac ccctttcttc agtggaaaca
      721 tcttgagcgc ctagtgtaat tcgattttgg ttatatgagc ctgttaacgc ttctaaataa
      781 acagcaggtg gacacactac agtcgtaact ctcggaagta cgcgagctat tttttttatc
      841 tccgtaaaaa aaaatttcgc ctcccaaaga gagtcaggat tcatcttcca gttagcaact
      901 atatattttc ttccgttccg atttttcata cgcaactatt atatcagact gctaacgcat
      961 ctaaacccct tcggaaaagt cacaaccacc tgcaccaagg ataaacttat tcaacctctt
     1021 tccaagaacc aaagacgtac ccaccagatg gaccacttga gaaaatagta ttaacgagcc
     1081 cgctatcgta ggtgactgtt gagccattat ccatgtcaat aagccttgcg gttgcttcct
     1141 tcgcgccaga attattgttg aaatgaatgg tgcctttctg agcgtttagt atgaccgtac
     1201 cagcattatt tccgacatca attgcggcag ggcttgttcc cgtatttgtc gagagaatca
     1261 taatatagct tccagtttgt ccagtgccat taaataagga gttattcgag atcgccgtag
     1321 tcccttcaac gacaatgacg gaactccgag taccgaatga tgatgcgagt gttactgttc
     1381 ttccgtttga agttgtcaca ttacctgtaa cccagagaat cccagtaaga gtaaagttgt
     1441 tatcgataac taaattacag gggatttttt tgggaccgag cgaggctccc gtggcaggag
     1501 tatagttacc tccagagcat gtgacctcgc catcggttac ggcatcattt ttccactgct
     1561 caatattgct ctctgagaca ggcatatttt gtgaaaccgg gtcaactgcc acactacaac
     1621 ttagagcgtt tgaattctcg tcagtgttac cagaaccagt ttggcaatac aagctaccat
     1681 tgacttttga attgttcact gcatgggccc aagcgtcacc tacaccggcc gttccgatat
     1741 ctatattatc tattacggca ggagcaccct caagataaat agtaaaatac ccatcaaggc
     1801 ctgctgggga cgtatcgccc caagagcctc caaacgcgcc tatgttagcg agaccgctcg
     1861 tgtaagaaga attcgcgcct attgtgtagt agtttgatgc tgaatctgtt ggactgtcga
     1921 caacaatcca gtaggtgatt ccagaagtta aatttacgtt acttgaaaaa acaatatcaa
     1981 ccagtccata agtagtagtc acaaaatttg aattgagtgt tccagtagta agactagccg
     2041 tgccgggtga accgcccgaa ttcgagacaa ttctcacagt tgcgctcgac gggtccccta
     2101 ctttctttat ataaagttgt acaagactat ttttaccaga actcgacaac acaaagctct
     2161 gagcgaaatc ttgcgtagaa gcaccgtcag caaaattgat agagtttgtt ggtggtgttg
     2221 gcgttatatt cgtttggtca ggactggcag tcggtggatt tgaggccacg gccgcaccag
     2281 ttatggttgc attattcgaa ccagtaatat tacctcctga gtacacgctt ccgtttacag
     2341 ttgcatttcc cataataaaa cctccttgtc cggtttgaat accgtaatta aatatggcgc
     2401 cgattcccgt agtcattatc gccttagctt ttctcacgaa gctgccgcaa gtggcagttg
     2461 aaataagttg ttttccgcct acgatgtcca cgacttcaac ttc
//
LOCUS       DATDSU010000076         3813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_73317, whole genome shotgun sequence.
ACCESSION   DATDSU010000076 DATDSU010000000
VERSION     DATDSU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73317"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VI981_02525"
     CDS             <1..642
                     /locus_tag="VI981_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479205.1"
                     /translation="VIGPDSLVLTSAPETLFEADPASAVFEPVVAETATTFSTRASFV
                     FFTDVGAHAACAVDAPRTKAAMSAEIAIFVIEILVIIVFVIFLLRPLREYGTRLVSTF
                     GHSVQAEGQFEIVLMLVLNYQDLGLGNYLYGDRLSHKVSQNKKPLVQLSARATLKTHY
                     MGNSLHKINPFSLGFPSREKLTDVHIICSPVGVSMPKYLLRNTNNATVNFTRG"
     gene            complement(906..1229)
                     /locus_tag="VI981_02530"
     CDS             complement(906..1229)
                     /locus_tag="VI981_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479206.1"
                     /translation="MNNELSQKIMQRVRFVFVMKRFVLPVSFKVAVFVASAVALWSFV
                     SLRDVVANMPGLDHAGSVMSFMLSAFASTEFAVQVTLVAMGLFFIWLMRDFAKNFTFR
                     FGVAS"
     gene            complement(1242..1820)
                     /locus_tag="VI981_02535"
     CDS             complement(1242..1820)
                     /locus_tag="VI981_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4479207.1"
                     /translation="MNLLNESEDLGQLSDEEILSLSTENPNHFELLVDRYQQKFIDRA
                     FYILRNKEEAADIVQDTFVKIYLNAKRFRPVPGASFKSWAYRILLNTCFTKCKKAKRE
                     REVFLRIEEETAEFIPDKAELVTAKQRLDTDQVLSMLSRLPVTLSRVMRLHYVEGKSQ
                     EDIATLEGVKNGVIRTRIHRAKKILKNLTTHH"
     gene            complement(2280..3692)
                     /gene="purB"
                     /locus_tag="VI981_02540"
     CDS             complement(2280..3692)
                     /gene="purB"
                     /locus_tag="VI981_02540"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY4479208.1"
                     /translation="MYTGSIVERYASSKMVAIWGPMEKVIMERKLWVTVLKAQDALGI
                     VGRQIPDGAIRAYEKNIANVDLEQIRVRELETRQDVTARIAEFNSLAGYECVHLGMTS
                     RDQTDNTEQCQILASLKIVRDKTVAILSRFAAKALQFKLRNMCGRTHNVPAQTIRLGK
                     RFANFAEELLIAFERLEHLIEVYPLRGIKGATGTQQDMLDLLGSPEMVKRLEEIVAEE
                     LGFRRVLESVGQVYPRSLDYEVLSVLTQLASAPGNFAKMVRLMAGIELAHEGFKEGQD
                     GSSAMPHKMNSRTCERINGLVHVLGGFGEMTKALIGDQWYEGDVSCSVIRRVALPGAF
                     FALDGIYESLMTVLDEMEVFYVMVERELERYLPFVSSTRLLTAVVKKGMDREKARKII
                     KKHATSAIRSMRGGGDNCFAEDLARDEDFPLDGGEIETLLATPKTGLAEEQVDAVCAK
                     VCVLVRRYPEAEVCNPEPLL"
BASE COUNT         1010 a          951 c          789 g         1063 t
ORIGIN      
        1 gtgatagggc ctgattcgct cgtgcttaca tcagcgcctg aaactttatt tgaagctgac
       61 ccagcgtctg ctgtgtttga acctgttgtt gctgaaacag ctacaacgtt ttcaacgaga
      121 gcttcatttg tattctttac tgatgtagga gcccatgctg cttgcgcagt tgatgctcca
      181 agaacgaaag ccgcgatgag tgctgaaatt gcaatttttg tcattgaaat tcttgtgatt
      241 attgtttttg taattttctt actccgtccc ttaagggaat acggaacaag acttgtatcg
      301 acctttggac attcagtcca agcagaagga cagtttgaaa tcgttctcat gcttgtactc
      361 aactatcaag atttaggact cggcaattac ttatacggcg atcgattgtc acataaagtt
      421 tcccaaaaca aaaaaccgct cgtgcagtta tctgcacgtg cgactctgaa aacacattat
      481 atgggaaact ctttgcataa aatcaaccct ttttctcttg gtttcccttc aagagaaaaa
      541 ctcaccgatg tccacatcat atgctcgccc gtaggagtgt caatgccaaa atatctttta
      601 cgaaacacta ataatgcaac tgtcaacttc acacgtggtt aagcgacaga gagccataag
      661 gtatacttat gaaaaacacg tgagcggacg ctaaatctgg gaaacaggct cttacgagcc
      721 aggattaagc gtccactcac gggttcttta ttaaaaagaa aaaggccaca cctataaagt
      781 gcttggcctt ttcgcatact atactcccgc cttaatttgt caataaatta aatttgtcac
      841 ttagtttttc tctaaaaaac acaaaacccc ctttgcagaa tgcgaagggg gttttgtaag
      901 ttactctacg aggctacgcc gaagcgaaac gtgaaatttt tcgcaaaatc acgcataagc
      961 cagatgaaga aaagccccat ggccactagg gtgacctgaa ccgcaaactc cgtacttgca
     1021 aaggcagata gcataaagct cataacagag cctgcatgat cgagtcccgg catatttgcc
     1081 acaacgtccc ggagagagac gaacgaccag agcgctaccg ccgatgccac gaacaccgcg
     1141 accttaaacg acacaggtag aacgaaccgt ttcatcacaa atacaaaacg gactcgctgc
     1201 atgatttttt gactcaattc gttattcatt gcacttaaat attaatggtg cgtggtcaaa
     1261 ttttttaaaa ttttctttgc ccgatgtatt cgggtgcgga tcacgccatt tttcacaccc
     1321 tcgagcgtgg caatatcctc ttgagatttg ccttcaacat aatgtaacct cataactcga
     1381 gaaagtgtta cgggcagcct tgataacata gaaagtactt ggtcagtgtc aagccgctgt
     1441 ttggcagtca caagttctgc cttgtccggt atgaattcgg ccgtttcttc ctcaattcgc
     1501 agaaaaacct ctcgttctcg tttggctttt ttacacttag taaaacatgt atttaaaagt
     1561 attcggtacg cccatgactt aaatgaagct ccgggaactg gcctaaagcg cttggcgttt
     1621 agataaattt taacaaacgt atcttgaaca atatcggccg cttcttcttt gttccgcaaa
     1681 atatagaacg cacgatcaat aaacttctgt tggtatcggt ctaccagaag ttcaaagtgg
     1741 tttgggttct cggtcgaaag tgagagtatt tcctcgtcag aaagctgtcc caaatcctct
     1801 gactcgttta gtaagttcat cccgttagaa ataggaaatg cttttgttta ttcaaaatac
     1861 acttactatt catactttgt aatacatttg gtgccatacg taattcaatt ataaagcatt
     1921 tccgtgccca atttttgaaa agtgaaatga gtccctccta ttttttcttt ttttactaag
     1981 tgattgtaca taaccaagtg atttctaacg ggattcatgt ctagtctaaa gaggggtagt
     2041 cacccctctt tagaaacatt aacttgtttt ctcgatctat acaaccccgc accaaaagga
     2101 acagccggct cgtgctttgc ccgaaaggga tgtgtctatt cgacacgcgc actcgccatg
     2161 gtgttggtgc ggggtttaat atttgcaaac catactttgc ttcgcaaaca tatgggcaaa
     2221 tattaaaaaa acacgcaatg tactgtattg cgtgcgtgtg aaagcgagaa cgacttatgt
     2281 cagagtagtg gctccggatt gcacacttct gcctctggat aacggcgcac cagcacacaa
     2341 acctttgcgc agacagcgtc cacttgttct tcggctaagc ctgttttagg cgtggcgaga
     2401 agcgtctcaa tttcgcctcc gtcgagcggg aaatcttcgt ctctcgctaa gtcttcggcg
     2461 aaacaattgt ctccaccccc gcgcatgctt cttatcgccg aagtggcgtg cttcttaatg
     2521 atctttcgag ctttttctcg atccattccc ttcttcacga ccgccgtgag aaggcgcgta
     2581 gatgagacaa acggtaaata tcgctcgagc tcgcgctcca ccataacata aaacacttcc
     2641 atctcgtcaa gcactgtcat cagagactca taaatgccgt cgagtgcgaa aaaagctccc
     2701 ggcaacgcca ctcggcgaat gacggaacag ctcacatctc cctcgtacca ctgatcgccg
     2761 atgagcgctt ttgtcatttc tccgaaaccg ccgagtacat gcacaagccc atttattcgc
     2821 tcgcaggtgc ggctgttcat tttgtgcggc atcgcagaag atccgtcctg gccttctttg
     2881 aagccctcat gagcaagctc aatgccagcc atgagacgca ccatcttggc aaaattgcct
     2941 ggcgcgcttg cgagctgggt aagaacagag agcacctcat aatcaagtga ccgaggatag
     3001 acctgtccca cactctcgag tactcgtcgg aaaccaagct cctccgccac tatctcttca
     3061 agtcttttca ccatttcagg actgcccaaa agatcgagca tgtcctgctg tgtgcctgtg
     3121 gctcccttaa ttccacggag cggatacacc tcaataaggt gctcaaggcg ttcaaatgcg
     3181 atcagcaact cttccgcaaa gtttgcgaag cgcttgccga gccgtattgt ctgcgcgggc
     3241 acattatgcg tgcgtccgca catattacgc aacttgaact ggagcgcttt ggccgcaaac
     3301 cgtgacaaaa tggccaccgt cttgtcgcgc acaatcttga gtgaggcgag aatctgacac
     3361 tgctctgtat tgtccgtctg gtcgcgggaa gtcattccaa ggtgaacaca ttcataccct
     3421 gcaagagagt taaactctgc gatccgcgca gtcacgtcct gtctagtttc aagctcgcgc
     3481 acgcgaattt gttcgagatc gacatttgcg atatttttct cgtatgctcg aatcgctcca
     3541 tcaggaattt gcctgccgac gatacccaaa gcgtcttgtg ctttgagtac ggtgacccag
     3601 agttttcgct ccataatcac tttttccatt ggtccccaaa tggcaaccat cttgctactt
     3661 gcatatcgct caactataga ccctgtgtac aactcaagat agctttatgt agagtcattt
     3721 tgtattttct cgtttttgtt ttatctactt ttccatcctc tgtgcgtata ccaaagatac
     3781 acttgcttaa gaaagtgtca taaacccact cga
//
LOCUS       DATDSU010000077         8702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1077690, whole genome shotgun sequence.
ACCESSION   DATDSU010000077 DATDSU010000000
VERSION     DATDSU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077690"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /gene="mnmA"
                     /locus_tag="VI981_02545"
     CDS             complement(<1..989)
                     /gene="mnmA"
                     /locus_tag="VI981_02545"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY4479209.1"
                     /translation="MKRQGAKKKVFIGVAPKENSYFPLGQAGGFRKTVYIGLSGGVDS
                     SVSAALLKKQGYDVTGVFIKVWQPNWITCTWKEDRLDAMRVCAKLEIPFLTLDLEKEY
                     KRDVVDYMIREYKAGRTPNPDVMCNKYIKFGAFYNWARKRGADYVATGHYARIEKVTI
                     RELSSINDQLSIENSLKIAVDTTKDQTYFLYGLTSEQLAHILFPVGHLKKPEVRKIAK
                     EFGLSTADKKDSQGLCFVGKVDMTEFLSHFIQKKNGNILDERGQKIGHHEGALFFTIG
                     ERHGFTVTKKSTHDRPLYVVSKDLKKNTITVSPRNREQNTNFDKNLVLLKNIT"
     gene            1019..1204
                     /locus_tag="VI981_02550"
     CDS             1019..1204
                     /locus_tag="VI981_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479210.1"
                     /translation="MKTTAHLKALLTLVIPLIAVLVLAPIIYKNWIDNVFTASFIAIV
                     VVGGSYSAATGLGKISN"
     gene            complement(1399..2535)
                     /gene="mltG"
                     /locus_tag="VI981_02555"
     CDS             complement(1399..2535)
                     /gene="mltG"
                     /locus_tag="VI981_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY4479211.1"
                     /translation="MHQDSENSGVERVPGNNKGSSGQSSEVLEELASLVHRPPEADSI
                     EPLIRPRSDGMPRVLDAPLLASRNKKIWIIASLILIFFAVCIYISTGPRNFEAHSVAI
                     VKGSSLGDAATTLKKENIIRSALLFRVLVIARGKAHSVIAGDYLFDGPQSIATVVSRL
                     TAGETGQSAEKVTIPEGTTTAAIALIVKRQISDFDTVGFLKIAQQKEGYLFPDTYFFN
                     HVITPEEVVEHMSENFEVKIADLAKKIETSGKPLKDVLIMASLIEGEARTAETRKIIS
                     GILWKRLKIGMALQVDAAFQYINGKSSSELTVDDLNIDSPYNTYKYPGLPPTPISNPG
                     TDAIIAALEPEESKYFYYLSDEEGAMHYAVTLEEHVMNKEKYLR"
     gene            complement(2539..3759)
                     /locus_tag="VI981_02560"
     CDS             complement(2539..3759)
                     /locus_tag="VI981_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479212.1"
                     /translation="MLDLLALTDSGVYRGIVVLLTLYPIWLPLVLAAVFWEHWLDYVR
                     ANFIKEQGSVLLEIRLPIDIVKNPKAMEIFYTALYQTGTATYIETYIKGKVKPWFSIE
                     MCSFGGQIKFFIWTWPKYRNLVETQLYGQFPSIEIIEAPDYTKNVHHDPVNLPLWGTN
                     FKMVRDQAFPLKTYFDYGLHTDPKEEYKIDPMTSTIEFLGSAQKGEQIWIQILFQAHK
                     KMGLKEGEIIHEPLWTKESRHEIEQMTNKLRGESEYPRPLTEGERDLVAAMERKVMKW
                     PFEVMIRGFYIATKEANRINLMIAGLLGSFRQYSAPDGFNEIKVGKFTDYDYPWQDFR
                     RQRRDMVEKKMLDAYKKRSYFNYPYENFRGKRYILSTEELATIFHFPGRVATTPTFDR
                     IVSKKGEPPANLPI"
     gene            complement(3869..4900)
                     /locus_tag="VI981_02565"
     CDS             complement(3869..4900)
                     /locus_tag="VI981_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY4479213.1"
                     /translation="MSTLKLKPKQVTKRLLAVLPERAQEVLESRYGLGKDHKRMTLES
                     IGKRYGITRERVRQIENYALNNIRKSENYTKERAAFEELENILHSLGGIVVEDDLLTH
                     ISIDKSIQNHLNFLLVLGESFKRQKEDEHFKHRWHIDDTLSKQVEESLKKLYRNISDN
                     DILTESEIVNTFLEHLKDISEKYKNEEIVRRWLNLSKKISKNPLGEWGSATSPNIKTK
                     GMRDYAFLVIRKHGSPIHFTQVAKTIQKVFNKKAHVATCHNELIKDPRFVLVGRGLYA
                     LAEWGYISGVVKDVIKKIIEKTGPLTKEEIIEKVLKERYVKENTILVNLQNQKFFKKD
                     RDGKYAVVA"
     gene            complement(5052..8039)
                     /locus_tag="VI981_02570"
     CDS             complement(5052..8039)
                     /locus_tag="VI981_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY4479214.1"
                     /translation="MKEYNHREIEKKWRGKWQKDGLYKTSDDLSRPKCYVLDMFPYPS
                     GEGLHVGHPKGYIATDIYSRFKRMNGFNVLHPMGWDAFGLPAENYALKTKTHPRDAVS
                     ANIERFKKQLSLIGFNYDWDREINTTDPAYYKWTQWIFLKLFEKGLAYEAEEPINWCP
                     KDMTGLANEDVENGKCERCGTLVEKKLLRQWKLKITKYAGRLLEDLDNLDWPISIKES
                     QRNWIGKSEGSTFKFEIRNSKSETNSNNTIGEIEVFTTRADTFYGVTYLVVAPEYPWL
                     IEQIQNPIRQSTDEISNKLEIDKYLNEVKNKTEIDRTDDKKEKTGVEIKGVKAIHPAT
                     GEELPVWVADYVLADYGTGAVMAVPAHDERDFEFAQKYKLAIREVVVPLFTREGTFDA
                     WRKNEKETIRPTVVCLIKHWSENKYLFLKKKIGSDSGADLIVAVAGGVDEGEDLILSG
                     KREIEEETGYNNAEFVKSLGTPVFYRYFSINAKQNRNSVLYPMYYKLKNETSVLVSDR
                     EKDLHDTIWLKEEEIPEKYFPGRGFNSIFWDRLQGVARPFLDEGMLISSGEFNHAASE
                     EGRSKIAKKYGQFKVTYKLRDWVFSRQRYWGEPIPMVHCEKCGIVPVPEKDLPVLLPE
                     VDRYEPTGTGESPLANITEWVNVACPKCGGAGKRETNTMPQWAGSSWYYLRYTDPHND
                     KELAEKGKIDYWLGDSWPLRSSAKRNDSGVDMYVGGAEHATRHLIYARFWHKFLFDIG
                     VVGMSEPFRKLQNVGLIGGADGRKMSKRFGNVVNPDRMVELYGADTFRLYEMFMGPFD
                     QAIPWNTDGMMGPRRFIERIWRLQTRVMDGKSDLESGKRSDLDHDVDVLLHRTIKKVS
                     DDIQAFKFNTAISALMILSNELEKKERISKDTYLSFLKLLAPVAPHVTEELWSLAGEK
                     AFIHEAEWPIYDEKQALQKKTTIVVQVNGKVRSSFDIDSDISREELEARAQTLPEIQK
                     WTENKGLKKIIVVPGRLVNIVVE"
     gene            8178..8591
                     /locus_tag="VI981_02575"
     CDS             8178..8591
                     /locus_tag="VI981_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479215.1"
                     /translation="MFASIKALLSGKTLFACSHSARRSASFRMDGEKVTFNLSDKQTE
                     WCPECLSKMTILCVFCARPIFIGDPICLPICKKNKVPPSAVLYEDFGSSGQEGQYVSC
                     LRFNCCDSGANRAGFWQPPGVVARVMRPVPQILDT"
BASE COUNT         2243 a         2050 c         1703 g         2706 t
ORIGIN      
        1 caagtaatgt tttttaacaa aactagattt ttatcaaagt ttgtattttg ttctctatta
       61 cgcggactga ccgtaatcgt atttttcttg agatcctttg atacaacata cagcggccga
      121 tcatgtgtcg atttttttgt gacagtgaaa ccatggcgct cccctattgt aaagaaaagc
      181 gccccctcat gatgaccaat cttctgacca cgttcgtcaa gaatattgcc gttctttttt
      241 tgaataaaat gcgataaaaa ttcagtcata tcaacttttc ccacaaaaca aagtccttga
      301 ctgtctttct tgtcagcagt acttaaacca aattcctttg caatctttct gacttcaggt
      361 tttttaaggt gacccacggg aaaaagaatg tgggctaact gttcactcgt caggccgtac
      421 aaaaaatatg tttgatcttt ggtggtatcc accgcaattt tcaatgaatt ttcaattgat
      481 aattgatcat tgatgcttga taattctcga attgtgactt tctcaattcg ggcatagtgt
      541 cctgtagcca catagtcagc gcctcgttta cgcgcccaat tgtaaaacgc gccaaacttg
      601 atgtatttat tgcacatgac atcaggattt ggcgttctgc cggctttgta ttctcgaatc
      661 atgtaatcga caacatcacg cttgtattct ttttcaagat caagcgtcag aaaaggaatt
      721 tcgagcttgg cacatacacg catcgcatct agtctatctt ctttccatgt gcaggttatc
      781 caattcggtt gccaaacttt tataaaaacg ccggtcacat cgtatccttg ttttttcagc
      841 aaggcggcag atacagatga gtcaactcct ccagacaaac caatatacac agtcttccta
      901 aaaccgcctg cttgccccaa aggaaaataa gaattttcct ttggggcaac cccgatgaaa
      961 accttttttt ttgctccttg tcgtttcatt aaacgcatgg tatcaaatac ttagcattat
     1021 gaaaacaact gcgcacttga aggcgctttt gactttggtc attcctctta tcgctgttct
     1081 tgtcctcgca ccgatcattt acaagaactg gatcgacaat gtattcacgg cctctttcat
     1141 tgcaatcgtg gtcgtcggag ggtcgtactc ggcggcaaca gggctcggga aaatctcgaa
     1201 ctaacaaccg gcaaagccgg tttttttata taaaattttt tgcatttttt tgttaaaatt
     1261 cactggcctt ttcaaataca ctaacatcac acgatcgtgt aatgttagtg tattgatatt
     1321 tttgaatatt ttttatattt aagtatttcg atcaacctca attaagagct gctttttttt
     1381 ctttttagat attagaaatt acctcaaata cttttcctta ttcattacat gctcctcgag
     1441 cgtaactgca taatgcatcg caccttcctc gtcagaaaga taatagaagt attttgattc
     1501 ctccggttcg agagcggcga taatagcatc cgttcccggg tttgaaatag gtgttggcgg
     1561 aagccccgga tatttatagg tattgtatgg agaatcaatg ttcaaatcat ctacggtaag
     1621 ctcactcgat gattttccat ttatgtactg aaatgccgca tctacttgga gcgccatgcc
     1681 gatcttaagg cgcttccata ggattcccga aataattttt ctagtttcag cggtgcgcgc
     1741 ttcaccttct atgagtgagg ccatgataag cacgtcctta agcggtttac cagatgtttc
     1801 tatttttttt gcgagatccg caattttaac ttcaaaattt tcgctcatgt gttcgaccac
     1861 ttcctccggc gtgatcacgt gattaaaaaa atatgtatct ggaaataaat atccctcctt
     1921 ctgttgagcg atttttaaaa atccgaccgt gtcaaaatca gaaatttgtc tttttacaat
     1981 gagcgcaatc gccgctgtcg tcgtgccctc gggaattgtc accttttctg ctgactgccc
     2041 agtctcaccg gcggtaagtc tcgagacaac tgtcgcaata ctttgcggac cgtcgaaaag
     2101 ataatcacct gcgattaccg aatgagcttt tcctcgagca atgacgagca ctctaaaaag
     2161 caacgcggaa cggataatat tttctttttt cagcgtcgtc gccgcgtcgc caagcgaact
     2221 acctttcacg attgcaacac tgtgcgcttc gaaatttcga ggaccagtcg agatatatat
     2281 gcacacggca aaaaaaatga gtatcaaact tgcgataatc catatttttt tatttcggct
     2341 agcgaggagc ggcgcgtcaa gtactcttgg catgccgtcc gagcgaggac gaatcaaagg
     2401 ttcaatactg tccgcctcag gcggacggtg gacgagcgaa gcgagctctt ccaatacctc
     2461 ggagctctgc cccgaggaac ctttattatt gccgggcaca cgctcgacac cactgttttc
     2521 agaatcctga tgcatacgct aaatcggtaa attggcaggg ggctcgcctt ttttggacac
     2581 gattcgatca aacgtaggcg ttgtcgccac ccttccagga aaatgaaaaa tagttgcgag
     2641 ttcttcggtc gataaaatat agcgtttgcc gcgaaaattt tcatagggat aattgaaata
     2701 tgatcgtttc ttgtatgcat cgagcatttt tttctccacc atatctcggc gctggcgacg
     2761 aaaatcctgc cagggataat cgtagtcagt aaatttccca acctttattt cattgaatcc
     2821 gtctggggcg ctgtattggc ggaaacttcc aagaagtcct gcgatcatga gattaattct
     2881 gttcgcctct ttggtagcaa tataaaaacc acggatcata acttcaaacg gccacttcat
     2941 gaccttacgc tccatagctg ccacaagatc tctttcacct tcggtgagtg gccggggata
     3001 ttcgctttca cctcgaagtt tgttggtcat ttgctcaatt tcgtgtctgg attccttagt
     3061 ccatagcggt tcatggataa tttcgccttc tttcagtccc atctttttat gtgcttggaa
     3121 aagaatctgt atccagatct gctcgccctt ctgggctgat cccaaaaatt caatggtcga
     3181 ggtcatcgga tcaattttgt actcttcctt cggatctgtg tgcaatccat aatcgaaata
     3241 agtctttaat ggaaaagctt ggtcacgaac cattttaaaa ttcgttcccc aaagcggtaa
     3301 atttaccgga tcgtgatgaa cattttttgt gtaatcgggc gcttcaatga tctcaatgct
     3361 cggaaattgc ccgtagagct gtgtttcaac gagattacga tatttgggcc atgtccagat
     3421 aaaaaatttt atctgcccgc caaacgagca catttctatc gaaaaccatg gctttacttt
     3481 acctttgata tacgtctcaa tataggtagc cgtaccggtt tggtagaggg ccgtataaaa
     3541 aatttccata gctttcggat tttttacaat atcgataggc aaacgtatct ccaggagtac
     3601 tgatccctgt tctttaatga agttcgcgcg aacgtagtcg agccaatgtt cccaaaagac
     3661 agcggcaagc actaagggca accatatagg atagagggtc aagagtacaa caatgcctct
     3721 ataaaccccg ctatcggtaa gcgcaagtaa atccaacatg aatacaataa tagctaatgt
     3781 cgctgaaaga taaaagtgat atcggcagag cagagtaacg acaaaacgcg ctccacgcgt
     3841 gttttatggt gagtatttcg cgcacatgtt atgcgactac cgcgtatttt ccgtctcgat
     3901 ctttcttgaa gaatttctga ttctgtagat tcacaaggat agtattctct ttaacatacc
     3961 gctccttcaa aactttttcg atgatttcct cttttgtgag aggaccagtt ttttcgataa
     4021 ttttcttgat aacatctttc acaacaccgc ttatatatcc ccattcggca agcgcataga
     4081 gaccacgccc cactaaaaca aatcgggggt cttttatgag ttcgttgtga caggtcgcga
     4141 catgggcctt cttattgaaa actttctgta tcgtcttggc gacctgcgtg aaatgtatcg
     4201 gggagccgtg ttttcgaatt accaaaaaag cgtaatctcg catgcctttg gtcttgatat
     4261 tcggcgacgt tgcgcttccc cactcgccga gcggattttt tgaaattttc tttgagaggt
     4321 tgagccagcg tcggacaatt tcctcattct tatacttttc agaaatatcc ttaagatgct
     4381 ccaggaatgt atttacaatt tccgattccg ttaagatgtc gttatcggaa atatttcggt
     4441 acagtttctt caatgattcc tcgacttgct tcgataacgt gtcgtcaata tgccatcggt
     4501 gtttgaaatg ctcatcttct ttctgtcgtt taaatgattc gccgagcacc agtaaaaaat
     4561 ttagatgatt ttgtatgctt ttatcgattg aaatgtgtgt caataaatca tcctcaacaa
     4621 caatgccccc tagtgagtgt aaaatattct caagctcctc aaacgccgca cgttctttcg
     4681 tgtaattctc ggattttcgg atattgttga gtgcataatt ctcgatctgt cgtacgcgtt
     4741 cacgggttat accatagcgc tttccgatag actcaagtgt cattctttta tgatctttcc
     4801 cgagaccata tctgctctca agcacttctt gggccctttc cggcaataca gcgagaagtc
     4861 tttttgtgac ctgcttcggt ttcaatttaa gcgtactcat atatggagtt cacaacaatt
     4921 agattgataa aattttgcga taaatttttt tcaccacgga gcaataaaat tcaaattatc
     4981 acttaacctt atactagtca agccgagccc ccttgtcaaa ttatgatttt cggggtactg
     5041 ggtaaggatt gttactcgac aacgatattg acaagacgcc ctggcacgac aattatcttt
     5101 tttaggcctt tattttcggt ccatttttgg atttcaggaa gcgtttgcgc tcttgcttcg
     5161 agttcttctc tcgaaatatc gctgtcaata tcaaaactcg aacgtacttt gccgtttacc
     5221 tgcacgacta ttgttgtttt tttctggagc gcctgcttct cgtcgtagat cggccattcg
     5281 gcttcatgta taaatgcctt ttcgcctgca agcgaccata gttcctcggt cacatgcgga
     5341 gcaaccggcg caagtagctt tagaaacgaa agatacgtgt cttttgaaat acgttctttt
     5401 ttttcaagtt cgtttgacaa aatcatgagc gccgaaatgg cagtattgaa tttgaatgct
     5461 tgaatatcgt cactcacttt tttaattgta cggtgcaaga gcacatccac atcgtgatca
     5521 aggtcggacc tttttcccga ttcaaggtcg gactttccgt ccattactcg agtctgaagt
     5581 cgccatattc tttcgataaa acggcgcgga cccatcatcc catcggtatt ccacggaatt
     5641 gcctgatcaa atgggcccat gaacatctca tagagtcgaa atgtgtccgc gccgtagagc
     5701 tctaccatcc gatcggggtt cactacgttc ccgaaacgct tactcatctt gcgcccatct
     5761 gcccctccga tcaatccaac attctgaagt ttcctaaaag gctccgacat gccaactact
     5821 ccgatatcaa aaagaaattt atgccaaaaa cgcgcgtaga taagatgccg cgtcgcatgc
     5881 tccgcgccac ccacatacat atcgacccca ctgtcgttcc gctttgcgga acttcgaagg
     5941 ggccagctgt cacccagcca ataatcaatt ttcccttttt ccgcaagctc cttatcgtta
     6001 tgcggatctg tgtatcttag gtagtaccaa cttgacccgg cccattgcgg catggtgttc
     6061 gtttctcttt ttccagctcc gccacatttc gggcatgcta catttaccca ctctgtaata
     6121 ttcgcgagcg gcgactcacc cgttccagtc ggctcataac gatcgacttc cggaagcaga
     6181 accggtaggt ctttttccgg cacgggtaca atgccgcatt tttcgcagtg taccatcgga
     6241 atcggttctc cccaataacg ctgacgcgaa aagacccaat cacgaagttt gtacgttact
     6301 ttgaattggc cgtatttttt tgcaatttta ctccgccctt cttccgaggc ggcatggtta
     6361 aattccccag acgaaatgag cataccttca tcaagaaaag gcctcgctac cccctgcaaa
     6421 cgatcccaaa atatagaatt gaagcctctt ccaggaaaat atttttctgg aatttcctcc
     6481 tctttaagcc aaatggtgtc gtggagatcc ttttcgcggt cggaaacaag aacagatgtt
     6541 tcgtttttca gtttgtagta catagggtaa agcactgaat ttctgttttg tttagcattt
     6601 atcgaaaagt atctataaaa cactggggtg ccgagagact ttacaaattc ggcattatta
     6661 taaccagtct cttcctcaat ctctcttttt ccggaaagaa tgaggtcttc gccctcgtca
     6721 actccgcccg caacagcaac tatgagatct gccccggagt cagaaccgat tttcttcttc
     6781 aaaaacaaat atttattttc tgaccagtgt ttaatcaaac aaactacggt cgggcgaatt
     6841 gtttcttttt cgttctttct ccaggcatca aaagtaccct cgcgagtaaa cagcggcaca
     6901 acgacttctc ttatcgccag tttgtacttt tgagcaaatt caaaatcacg ctcatcatgg
     6961 gctggaactg ccatgacggc gcctgtgccg tagtctgcca gtacgtaatc ggcaacccac
     7021 actggcaatt cttcacctgt agccggatgt attgctttta ccccttttat ttcgactcct
     7081 gttttctcct ttttatcatc agttctatca atttctgttt tattttttac ttcgtttaga
     7141 tatttatcaa tttcgagttt gtttgaaatt tcgtccgtcg actgacggat cggattttga
     7201 atttgttcaa tgagccatgg atattccggc gcaaccacta aatatgtcac tccgtaaaac
     7261 gtatcagctc tggtcgtaaa gacttcaatt tccccaattg tattattcga atttgtttcg
     7321 gacttcgaat ttcggatttc gaatttaaaa gtggatcctt cactttttcc tatccaattt
     7381 ctctggcttt cttttataga aatcggccaa tctagattat caagatcttc gagaagtctg
     7441 ccagcatatt ttgtgatctt caatttccat tggcgaagca actttttttc cacgagcgta
     7501 ccacagcgct cgcattttcc gttttccacg tcttcattgg caagtcccgt catgtccttc
     7561 ggacaccaat tgatcggctc ctctgcttcg taggcaagtc ctttttcaaa aagctttaaa
     7621 aatatccatt gcgtccattt gtaatacgcc gggtcggtag tattaatttc tcgatcccag
     7681 tcatagttga agccaatgag tgagagctgt tttttgaagc gttcaatatt cgccgaaact
     7741 gcgtcacgcg gatgagtctt cgttttaaga gcgtaattct cggcaggcaa accaaaggca
     7801 tcccatccca taggatgaag cacattaaag ccattcatgc gcttaaaccg cgaatatatg
     7861 tcagtggcaa tgtagccttt gggatgtccg acatgaagcc cttcgccaga aggatacggg
     7921 aacatgtcga gcacgtagca ctttggacgc gacaaatcat cactcgtttt atagagtccg
     7981 tctttctgcc actttcccct ccacttcttt tctatttctc tgtggttata ttctttcatt
     8041 gacctttatt cagattactg taacacaaag ccgaatgcac ataaataatt gcgtaggtta
     8101 gggttgtact actttgcatt tttttgttgc acaatttccc cattatagta ctctcatatg
     8161 agaaaccaca ttcacttatg ttcgcatcaa ttaaagctct tctttccgga aaaacactct
     8221 tcgcatgctc tcacagcgcg agaagatccg catcgttcag aatggatggt gaaaaagtca
     8281 ctttcaatct gtccgataag caaactgaat ggtgccccga atgcctctcc aagatgacca
     8341 ttctgtgcgt tttctgcgcc aggcctattt tcatcggcga cccaatttgc ttgccaatct
     8401 gcaagaaaaa caaagtgccg ccaagcgccg tactctatga agattttggt tcgagcgggc
     8461 aagaaggtca atacgtctcc tgcttgcgct tcaattgctg cgacagtggt gcaaatcgag
     8521 ccggattttg gcagccacca ggtgttgtgg cacgtgtcat gagaccggta ccccaaatct
     8581 tggacacata gtatctctca ttaacagagt aaaatatgtg tcatggaaaa cgattcaata
     8641 attaattaca aaagcaaacg tggtgttcac gttcccaaag acgtaaagga atatattttg
     8701 aa
//
LOCUS       DATDSU010000078         3560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1078050, whole genome shotgun sequence.
ACCESSION   DATDSU010000078 DATDSU010000000
VERSION     DATDSU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078050"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VI981_02580"
     CDS             <1..787
                     /locus_tag="VI981_02580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051763.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY4479216.1"
                     /translation="MDQGKDALVAYDDLTKHAWAYREISLLLRRPPGREAYPGDIFYV
                     HSKLLERAAKLSKEFGGGSLTALPIIETQLSDISAYIPTNVISITDGQIYLESELFYQ
                     GIRPAINVGLSVSRVGSSAQTKAMKKVAGTLRLDLAQFRELAAFAQFGQDLDAATKKK
                     IDRGMRLTELLKQVQYSALPFEEEVVSIFAAVNGHIDTVPVSEVSRFEKEMLSFIRER
                     HKKIFEKIRSGGELTEEITEELAAVIKDFSEHYFKANALNPKP"
     gene            799..1725
                     /gene="atpG"
                     /locus_tag="VI981_02585"
     CDS             799..1725
                     /gene="atpG"
                     /locus_tag="VI981_02585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY4479217.1"
                     /translation="MATSRVLKKRIRSTKNIKQITKAMQAVAAVKMRRSEEVALSARP
                     FAHAALEILRNVTASIGNERALSPFLRSGKGEKPLLVVVTSDKGLAGSLNTNVLRVAD
                     TYLKNATQEVSIVAVGKKGRDYYTRRGKKVIKEFVDFGDSVKIEEVQPLTAYLKNQFI
                     ETACDEVLVIYTNFITALRQEVITRRLLPFDRKTLQEIVEGIAPLQGKYSGPTENLGQ
                     ENREFVFEPSPQEVLSELLPLLFDIEMYQAILEANASEHSSRMVAMKNASENASELIT
                     ELTILYNKSRQAQITRELSEITAGTEALANAN"
     gene            1730..3115
                     /gene="atpD"
                     /locus_tag="VI981_02590"
     CDS             1730..3115
                     /gene="atpD"
                     /locus_tag="VI981_02590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY4479218.1"
                     /translation="MSNMNKNGKISQIIGPVVDVDFETDVPAIHSALQIVVDGKAGLV
                     LEVAQHLGGGRVRAIAMGATDGLRRGDDVKYISESITVPVGKETLGRMFDMLGNPIDG
                     LGEVKGIKEHWPIHRKAPAFTEQSTEVKIFETGIKVIDLIAPFIKGGKIGLFGGAGVG
                     KTVLLQELIRNVATVHKGYSVFAGVGERTREGNDLYHEMKDSGVLGQTTLVFGQMNEV
                     PGARLRVALSGLTMAEYFRDTEGRDVLLFIDNIFRFTQAGSEVSALLGRMPSAAGYQP
                     TLASEMGELQERITSTKKGSVTSVQAIYVPADDITDPAPATTFAHLDSTIVLARSLAE
                     LGIYPAVDPLDSNSTALDPNIVGTEHYKVAREVQRVLQRYRDLQDIIAILGIEELSDD
                     DKLLVARARKIQRFLSQPFMVAEAFTGRQGKFVSRADTVKGFRDIIEGKLDDVPEQNF
                     YMIGTIDEARK"
     gene            3204..3443
                     /locus_tag="VI981_02595"
     CDS             3204..3443
                     /locus_tag="VI981_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY4479219.1"
                     /translation="MRVFIHSLEKTLYEGDGQVVVLPARGGEISVMQNHIPLVAPLKQ
                     GSILVKEGERGEKKFTVSSGFVEVKPSHVIVLLNP"
BASE COUNT         1022 a          767 c          853 g          918 t
ORIGIN      
        1 catggatcaa ggcaaggacg cgctcgttgc ttacgacgac ctcaccaagc atgcatgggc
       61 ataccgcgaa atttctttgc tcctccgaag gccgcccgga cgtgaggctt accccggaga
      121 cattttttac gttcattcaa aattactgga gagggcggcg aaacttagta aggaattcgg
      181 cggaggctca ctgactgcac ttcccattat cgagacgcaa ctttccgata tttcagcgta
      241 cattccgacg aacgtgatct ccattactga tggccagatt tatcttgaat cagaactctt
      301 ttatcaggga ataagaccag ccataaacgt aggactttcc gtttctcgtg ttggttcttc
      361 ggctcaaaca aaggctatga agaaagtggc aggcacactt cgtcttgacc ttgctcaatt
      421 tcgggaactt gccgcttttg cacagtttgg acaggacctt gatgcggcta caaaaaagaa
      481 aattgatcgc ggcatgaggc taaccgagct tttgaaacaa gtccaatatt cggcacttcc
      541 ttttgaagaa gaagtggtat caatttttgc cgcggtgaac gggcatatag atacggttcc
      601 ggtaagtgag gtttcccgtt tcgaaaaaga aatgcttagc tttatccgcg agcgtcataa
      661 aaaaatattt gaaaagatcc gctcgggcgg tgaattgacc gaggaaataa ccgaggaact
      721 tgcagcagtc attaaagatt tctccgaaca ttactttaaa gctaacgccc taaaccctaa
      781 accctaaacg ctattcctat ggccacatca cgagttttaa aaaaaagaat acggtcgaca
      841 aaaaacatta agcaaattac aaaggcgatg caagcggttg ctgctgtcaa aatgcgtcgc
      901 agtgaggagg tagccttgag cgcccgcccg tttgctcacg cagcactcga gatacttcgc
      961 aatgttacag caagtatcgg aaacgaacgc gcgctttctc cgtttcttcg ttccggtaag
     1021 ggagaaaagc cgcttctcgt agtcgttact tcagacaaag ggcttgcagg gtcactgaat
     1081 acaaatgtgc tccgcgttgc tgacacctat ttgaaaaacg ctacacaaga agtatctatt
     1141 gttgcagtgg gaaaaaaagg aagggactac tatactcgcc gcggtaaaaa agtgatcaaa
     1201 gaatttgtgg attttggcga ttcagtaaaa atcgaagaag tccagccgct taccgcatat
     1261 ctcaaaaatc agttcattga gacggcgtgt gacgaggttt tggttatcta cacaaacttt
     1321 atcactgcgc ttagacaaga agtgataacg agacgactcc tgccttttga ccgtaagacg
     1381 ctccaggaaa ttgtggaggg tatagcaccg cttcagggta agtattcagg gccaacagaa
     1441 aatcttggac aggaaaacag ggaatttgtg tttgaaccct caccgcagga agtactaagc
     1501 gaacttttac ctttgctctt tgatatcgaa atgtaccagg caatcttgga agctaacgcg
     1561 tcggaacatt catctcgcat ggttgccatg aagaacgcat cagaaaacgc gagtgagctt
     1621 atcaccgaac taaccattct ttacaataaa tcacgacaag cgcagattac gagggaactt
     1681 tctgaaatta cggcgggaac agaggcctta gctaatgcaa attaataata tgtcgaacat
     1741 gaacaaaaac ggaaaaattt cacaaattat cggtcctgtc gtagatgttg attttgaaac
     1801 agatgttcct gcgattcact ctgcactcca gattgttgtt gacggaaaag caggtctagt
     1861 acttgaggtt gcacagcatc tcggcggcgg gcgtgtacga gccatcgcca tgggtgcgac
     1921 cgacggactg cgccgaggcg atgacgtaaa atatatttcg gaatctatta cggtgccggt
     1981 aggcaaagaa actcttggcc gcatgtttga tatgctcggt aatccgatag acgggctcgg
     2041 agaggtaaag ggcataaaag aacattggcc gattcaccgg aaagcccctg cttttacgga
     2101 acaatcgact gaagtaaaga tatttgagac tggaattaaa gttattgacc tcatcgcacc
     2161 ttttatcaaa ggaggtaaga tcgggctctt tggaggcgct ggggtgggca aaacggtgct
     2221 tctgcaggag cttatacgaa acgttgccac tgtacacaag ggttattctg tttttgcagg
     2281 agtcggagag cgaactcgcg agggaaatga tctttatcat gagatgaagg attcaggcgt
     2341 gcttggccag acaactcttg tttttgggca gatgaacgaa gttccgggtg ctcgtctaag
     2401 agttgctctg tcaggtctta ccatggcgga atattttcgt gatactgaag gaagagacgt
     2461 gttgcttttc atagacaaca tttttcgctt tacacaggcc ggttccgagg tctcggctct
     2521 tttgggtaga atgccttccg ccgccggata ccagcctacg cttgcatcag aaatgggtga
     2581 actgcaggaa cgcattactt caaccaaaaa aggttctgta acatcagtcc aagccattta
     2641 cgttcctgcg gacgatatta ccgatcctgc tcctgcgacg acgttcgcgc acctcgactc
     2701 aactattgtg ttggctcgtt ctctcgccga acttggtatt tacccagctg tcgacccact
     2761 tgattcaaac tcaaccgcgc ttgatccgaa cattgtggga acggagcact ataaagtggc
     2821 gcgtgaagtt cagcgtgtgc ttcagcgcta tcgagatttg caagacataa tcgctattct
     2881 aggcattgaa gaactctctg acgacgacaa gttgcttgtg gcgcgtgcaa gaaaaattca
     2941 gcggtttttg tcgcagcctt ttatggttgc cgaggcgttt accggaagac aaggaaagtt
     3001 cgtgtcgcgc gccgatacgg tcaaaggctt cagagatatt atcgaaggta aattggatga
     3061 tgtacctgaa caaaatttct atatgatcgg aacgattgat gaggcaagga agtaaaaaat
     3121 atcaaattga taataacaaa tatctaatga gaaataataa atcgcttttg aaattagaaa
     3181 ttagaaatta gaaattacat tccatgcgtg tatttatcca ctcactcgaa aaaacactgt
     3241 acgaaggaga cggccaagtc gttgttttgc ctgcacgggg cggcgaaata agtgtaatgc
     3301 aaaaccatat tccacttgta gctccgctta agcaagggtc aatactagtt aaagaagggg
     3361 agcgcggtga gaaaaaattt accgtctctt ctggttttgt ggaagtaaaa cctagccacg
     3421 tcatcgttct actcaatccg tagatattct catataattt tttcatattg aagattctct
     3481 ggagtactat aggtgtacta acttcgtgct atagccactt tgactgaaaa atttggttga
     3541 tggggttact acaatacctt
//
LOCUS       DATDSU010000079         3923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_234082, whole genome shotgun sequence.
ACCESSION   DATDSU010000079 DATDSU010000000
VERSION     DATDSU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234082"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..139
                     /locus_tag="VI981_02600"
     tRNA            68..139
                     /locus_tag="VI981_02600"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Asn,seq:gtt)
     gene            complement(1603..2313)
                     /locus_tag="VI981_02605"
     CDS             complement(1603..2313)
                     /locus_tag="VI981_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479220.1"
                     /translation="MAKISDSFEVHKLDDLQLKGLWVLDKLSDSKDRLSSVEIADYLI
                     EECGISTSRQAITYALEKEGGIVHKNKGGYKLMEAGRKKLAAGNSNSVIVIESGKPFS
                     TKNVIVKDIFNNLKSPVLISDPYLDINTLDLLFKNLDKKKQTKILTHKIADKPSGTFS
                     RHLGELRQEGFQIEIGVYSNSDLHDRYIMDDNSFWLSGNSLNYIGNKESFIVRLGEDI
                     RQSMLATFNNRWKIANKI"
     gene            complement(2319..2738)
                     /locus_tag="VI981_02610"
     CDS             complement(2319..2738)
                     /locus_tag="VI981_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479221.1"
                     /translation="MDDIKQLNEKVSSLEKRVFVLEAKLDNNLVESGVAIDKKLSVKE
                     FILSKGPSGDVQKTLAIACYLEKYEGFSSFNAGDLVHGFRLAKEPVPENINDKINMNI
                     KKAHMDEAKEKKDNKKAWIVTNSGERFMDNGFKEIKK"
     gene            complement(2744..3124)
                     /locus_tag="VI981_02615"
     CDS             complement(2744..3124)
                     /locus_tag="VI981_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479222.1"
                     /translation="MTDKKNTIDDLFSDSGPFDEAEVVKAIQPFVTIQKATNEIFFKS
                     SSQTTEQRILIYGLAKKLLKTKGLIESEMITAVEVQKKTSIKRGSIDPTFKKLKENGF
                     LVGKIDYEIPIPKIPDVIKVINKK"
BASE COUNT          923 a          944 c          866 g         1190 t
ORIGIN      
        1 gcgctcctcg cgaaaggaaa atgacgtctt ttgtctacgg caaacatcgg ctataatccg
       61 cgtacattcc ccggtagcgc aatggtagcg cagctccctg ttaaggagtt ggttgtaggt
      121 tcgagtccta cccggggagc aaggataaaa cttaggtcat ttttagagaa tctttttcgt
      181 gcgacgcgga gcgtcgccca ttgattcatc gggttttctt ggacttcttt gtttcgcaaa
      241 gtcaggttcg agccgaagat tttttggagg gaggattttt tggaggggag gtcgtccgtt
      301 tgagccgttt cgtccagtag cgaagcgtct tttatccacg ctcgcatggg ttcgagccac
      361 gcactcgccc ccttttccaa cttggcgact tgttcctcaa ccgacttccg atccgatacg
      421 agagaatggc gacgctctaa gtagtcgtcg cgttcgatgt cttgcgctat atagaggtcg
      481 gtaagacggg agagttcgcg tgaaaggttt tgagacttgg tgcgtagcac ttccacgaac
      541 gccgccgtct cgtgcgagga cttttccgcg tcctcgtcca tgaggcggta tagctcctgt
      601 gcccacggcg agggcatggc gtagccggaa aggacggcgt tcagttgcgg cactaaaact
      661 tcttcgcgaa tgtatggctg ggagcaaaca cctttctttt ttgtacagcg atagtatgta
      721 tggcctttct gcacttcggc ggtaatcata cagccgcact cgccgcaata cagcaagccg
      781 caaagcggct tcggttctaa aactggtttc tgcgggtggc ctcgctcggt cataactttc
      841 tgcactttgt cgaaaagact tttttcaaca atcggcgtat gccgcccttc gtgcaactct
      901 ccggcatatt tgaataggcc gcaatagaac ggattggaaa ggataaactt cacgcggtct
      961 ttgtgcgtct tgttgccgag gtggcttctc acgccgttat ccgctaggaa ggtagaaata
     1021 tcttcaagac gtgaattgcc tttggcgtat agctcaaagg ccgcgcgtat cgctttatat
     1081 ttcttgcggt caatgacaat cgtcttggcc cgaacatcgt tcaagtagcc gacgggagca
     1141 gtacccggaa actcgccgtg cttcactttg agacgaaggc cacgctttac gttcgcgctt
     1201 aggtcgcgga taaattggtt ggccatgccg aactcaacag acatcataag gacgttgtcg
     1261 gtcgggtaat ggcttcgctc atacgtccgt atgtgctgaa tcgtcccctg ttggagtagc
     1321 caactgatct gtccgccgtc tacgggatta cgggccaagc ggtcgagttt ccaacagata
     1381 acgctttggg cttcgccctt ctgaatacgc tttagcattt cgttaaagac aggacggccc
     1441 ggcattttgg ccgttcgttt ttccacgaat tcggatacaa tctctaaccc ttcgcttttg
     1501 gcgagagtgc gaagctcgga cagttgggct tcaatggaca taacctgcct gtcttcgaca
     1561 tcggtgcttt tgcgggcgta gagaaaatag ttgagcataa aattaaattt tgttggcaat
     1621 tttccacctg ttgttaaaag tagcgagcat actttgtctt atatcctctc cgagccggac
     1681 aataaaactt tctttattgc cgatataatt caagctattt cccgaaagcc aaaacgaatt
     1741 atcgtccata atatagcggt cgtggaggtc agagtttgaa taaaccccta tctcaatttg
     1801 aaaaccttct tgccgtagtt cccctaaatg acgcgagaat gtaccggaag gcttatctgc
     1861 aattttatgg gttaaaatct tggtttgttt tttcttatca agatttttga aaagaaggtc
     1921 gagagtattt atatccaaat aagggtcgga gataagaacg gggcttttga gattattaaa
     1981 aatatctttc acgataacgt tcttcgtcga gaatggttta cccgattcaa tcacaataac
     2041 gctgttagaa tttcctgcgg ccaatttttt acgtcccgct tccattaatt tatagccgcc
     2101 tttatttttg tggacgattc ctccttcctt ctccagcgca taagtaatag cttgtcggga
     2161 ggtacttatg ccgcattcct cgattaagta atcagcgatc tcaaccgaag acaatctgtc
     2221 ttttgaatca gaaagtttat caagaaccca aagtccttta agttgtaggt cgtctaattt
     2281 atgaacttcg aaggagtcag atattttcgc catgtcagtt atttctttat ctctttgaag
     2341 ccattgtcca tgaatctctc gcctgaatta gtcactatcc acgccttctt gttatccttc
     2401 ttctctttcg cttcgtccat atgagccttt ttaatgttca tatttatctt atcgttaatg
     2461 ttctctggga caggctcctt tgcaagccga aacccatgta ctaggtctcc ggcgttgaaa
     2521 gagctaaatc cctcatactt ctccagatag caagctatag cgagcgtctt ttgaacatcg
     2581 ccggaaggcc ctttagaaag tatgaattcc ttaactgaca gcttcttatc aatcgccact
     2641 ccggattcaa caagattatt atctaacttg gcctccaaga cgaaaaccct tttttctaaa
     2701 gaagaaactt tttcgtttag ttgtttaatg tcgtccatat tttttatttt ttgtttataa
     2761 ctttaataac atcgggaatc ttaggtatcg gtatttcata atcaatctta ccgaccaaaa
     2821 agccgttctc cttcaacttc ttaaatgttg gatcaatact acctctttta atgctcgtct
     2881 ttttttgaac ttcaacggcg gttatcatct cgctctcgat aagtcctttt gtctttaaga
     2941 gtttcttagc aagtccgtaa atcaaaatcc gttgttcagt tgtttgggaa gaactcttga
     3001 aaaatatctc attcgtcgcc ttctgaatcg taacgaaagg ttgaattgct tttacgacct
     3061 cggcttcatc aaaggggccg ctatccgaaa ataagtcatc tatcgtattt tttttatcag
     3121 tcatagtatt ttataagttt ttaagcgttc gccgctttag ccttttaatg gcctacggcc
     3181 tattatagag gaagtattat aaaaaagcaa gtaaagggta aaataaactt gccttttact
     3241 atgatttcgg ggagccgttg gtctttgacg acgggatacc tagtgcgcgc atgggtaggg
     3301 tggggcaagc actcctccag tgtgtcgcct tcgtctttta gttcggattt accctccaaa
     3361 aaaatctccc tccaaaaaat cttcgccttc gtatttttga acctgtattt tgcgaaacaa
     3421 agaagttcaa gaaaacccga tgaatcaatg ggcgacgctc cgcgtcgcac gaaaaagatt
     3481 ctctaaaaat gacctaagtt ttatccttgc tgggctgtac gacccaattc gacgatattt
     3541 taccagaaat gcctagtcac agggtagtca tttaggtagt cattcggggt ggtgcggaat
     3601 gactaccctc cgtctctgaa agtgcccgtc gttcttcgct cttttgaggg tgcttctgcg
     3661 aaatctcaag tcctacttca ctccggttta ccccttcaac caccccttca atccggcatg
     3721 ccccgaatga aggggtacgc cgcctcaatc tccccgcaaa gactggcctt tctgacatca
     3781 tgccctcata ttcccaagtc ctacttcact ccgctttgtg cctccgttcg agcctgtggt
     3841 atgaccccac ttttctggtc gcagagtccc tgcctagacc gcccttcggg cggttgcagt
     3901 cgggcggcaa ttccttcccc cca
//
LOCUS       DATDSU010000080        13275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_75843, whole genome shotgun sequence.
ACCESSION   DATDSU010000080 DATDSU010000000
VERSION     DATDSU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75843"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VI981_02620"
     CDS             complement(<1..292)
                     /locus_tag="VI981_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479223.1"
                     /translation="MIYRFKKNSNQWKRGQVMLVSVLVVTSVSLIVILGLASTINEHL
                     ARGGTLLLSGKSYALAESGIEDLAYRIKRNYASNATEVISLDGNSTEVEVVDI"
     gene            complement(294..815)
                     /locus_tag="VI981_02625"
     CDS             complement(294..815)
                     /locus_tag="VI981_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479224.1"
                     /translation="MKSLIQKKGFAIVEMLIYVGITALVITLMCYLIVSITNSSGPIK
                     LNKDLAVNADNTIGRMMLEIRNAASIDTGSSVLDATPGTLKLNTVDSSGAATTIEFYL
                     QNDAIMAKEGTGVGVSLMATGTKALSLIFRRISTGRSEGVKIETQLETTVRQRVKKEH
                     FFGTAVIRGSYQN"
     gene            complement(821..1330)
                     /locus_tag="VI981_02630"
     CDS             complement(821..1330)
                     /locus_tag="VI981_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479225.1"
                     /translation="MQVFHKKQKGSLLVEVLVGTSIFLLTVLSLHDVLTLFLKASRYT
                     LPFVKGGLLIDEGIEAVRSLRDQSWVNTVGTSTSGTFYLSWASSTARWQMQIPTVGKA
                     VPFITERYERSFTISSVNRDDTTKDIVSSGGALDSGTKKITMQVAWKNGNATTTKQTS
                     FYLTDLFAN"
     gene            complement(1362..1820)
                     /locus_tag="VI981_02635"
     CDS             complement(1362..1820)
                     /locus_tag="VI981_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4479226.1"
                     /translation="MIKKKGMTLVEVLISVVVLGFMSAIIITGLFSYRKHQAVDQTAH
                     TVASYLEEARRKTLASENSARYGVHFDTYQVVLYKNTYSSTEPSNRIYTLDSYSQINS
                     ISLLGGGSEVLFERLTGETQATGTITVSLRSDTSKTKKITISQTGVIEKN"
     gene            complement(1817..3028)
                     /locus_tag="VI981_02640"
     CDS             complement(1817..3028)
                     /locus_tag="VI981_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY4479227.1"
                     /translation="MPTFIYKIKKADGTIREDTRDAIDKYSLYRDIRKEGETVVSVTE
                     TAVKKSLWKRLADVSTIKQQEKIVFSRNMASMLEAGLPMSRALTISERQSRNRRFKLI
                     IGELNNEVKAGKSFSEALKKYPRIFPDIFSAMIRAGEESGTLAASLKRLSDQMDQVYK
                     LHKRIKGAMIYPSIILILMVIISIVLLVYVVPTLTSVFKDMDVTLPITTLFIIGASDF
                     VKNHYLLLVVFFISFFGGLYFFNKKQGTKKFLQLILLHIPIVGGIIKESNAAQTARTL
                     SSLLSSGVDYLRAIEITRDVIQNLHHKKVLEEAREKIQKGEPISSTFKKNEHLYPAFV
                     GEMINVGEETGKLAEMLADVALFYENEVEQRTKDMSTVIEPFLMIIIGASVGFFAVAM
                     IQPTYSLLNAI"
     gene            complement(3030..4784)
                     /locus_tag="VI981_02645"
     CDS             complement(3030..4784)
                     /locus_tag="VI981_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY4479228.1"
                     /translation="MYIEEKQLKDFLLDSGLVSKQDVLAAYEEAVGKKTSVGSVLVSQ
                     GKLSEDDLRKMQGYILGVPFVDLKNEHLDREILSLIPEPIARSHNIVAFKRSSDTLEV
                     AMTDTDDLAAIDFIKKKVKLRILARLTDSDSIKSALLQYQKSLKAEFGDIIQKEAEKV
                     KALTAKMSVATGETDLKKMAEELPVIGIVDALLKHAILQNASDIHIEPTDESLLIRYR
                     IDGLLHDAMILPKESASSIIARVKVLAQMKLDEKRLPQDGRFAQDLSGENVSYRVSLI
                     PTYHGEKAVLRVLRENAAGFTLEKLGFHGEGLESLHQALKASSGLILTTGPTGSGKTT
                     TLYTMMDILNTPDVNISTVEDPIEYQMKRINQTQVKPEIGFTFASGLRTLVRQDPDII
                     MVGEIRDVETAGLAINAALTGHLVLSTLHTSSAATSFPRLADMKAEPFLVASTVNVAI
                     AQRLVRRLAEIKQQHFLSSAEIESIGKHANLDLVLSVLKKEQIIGANLDWDKIPFYKS
                     KPSRDSEDGYKGRVVISEVLKVTNTIRDLIVKLETADTIEKQAREEGMMTMLEDGLFK
                     AAEGITTIEEVLRTVSSE"
     gene            complement(5160..9341)
                     /locus_tag="VI981_02650"
     CDS             complement(5160..9341)
                     /locus_tag="VI981_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479229.1"
                     /translation="MRIHHSKKLFKFLFVLFIVTPSIVGIFHPTETVEASAVTNHQRF
                     SNLDYKSAGVGGLRNATSVGINLTGVSGTVTKAFLIWNSVSNGSVGNTTSQLVTIAHS
                     GTAISQRVTGIQTGVSDGNCWDTYTAPIGPWFKSHSFMADVTGTVSGDGRYIISAFSN
                     KGNLNPNGASLLVFFDDGNAANNNNYILTTGNDSNKVSAYDAEGWAISIPDVNAPDGN
                     GTLELHVADGQTYPDPAVKINGSTLMAGGSIFQGRTVPGPNNGPIDNGNLWDIRSWSI
                     SPMLDIATQPVNLTHGYDDTGGDCVSFVAGVITLATIPPVPAPTVTLSANPTSVPSGS
                     ASTLTWSSTNSNSCTATALPTNSNWNGVKAINGNQSSGPLTVTTDFLMNCAGPGGVSP
                     VASAVVTVPVTGTCSVAPTSADTGQNVTWTSTATGGTGIYTYSWSGTDLLSGVSSSVV
                     KSYTAAGTKTGSVTVTSGTASTTIVCDNSVIVTAPPSFDYGLQNSGTITVTQGLSGSN
                     TITLTLITGMAQPVTLTVSGLPAGATAVFDNNPCTPNPSCVSSLTITTLASTPSGSYP
                     IVVSGNSPTKTTTFTLIVNTATQNGSVKGMLVDGLLGAMPGTTWNIDGGVAQNHGSTI
                     VVLSGSHTINATKLSGFTVGVGSCLFAPAECFVNTDVQYSTTNLGLDCAGAACSANIT
                     VPPNNTIKVAFKYVGAPGNLRVNATPAPCNGAINLSWNSVGGATSYNVYRSLSGIPMS
                     FTKINSTPVIVTSYTDTGLTLGMTYYYAVSTVDGSIESPLSSVVSGVATGACSTTGSV
                     KSIRVDQTFNVLPSAANLNNTLWMLDGGAVYGPSSSVNPELRSGVPSGTRIISVSDLA
                     GYTEEISVCNYPSSGPECFMTNPSTYSSVGVTCATGFCSASIPVAVDAVTKVAFRYVR
                     IVVPTGSIRTVRVDENFSTLPSALNPNTTSWGIDGTVISTVNPETRTNISLGNHTIFV
                     TDLPGYTEQWNTCDYAEGATECRMTDVSQYQTAGLTCSQGFCSVNEPVAANRVRKVAF
                     RYVRDVGPILGQVLNARLDNNLALMMPFGRTSWGLDGIPTSAANPAHDTDISIGGHSA
                     NVTNLPNFVERVGSCTYPVGGVECPITSYSTTGVTCTTTVCSANVVVLGNTVTKVFFT
                     YGVPSPPGGVTVDPGATCGSVVVRWDPVPDSTGYNVYRSTNGINFALRAGTIAAQYLE
                     SGLTAGVMYYYRITAVNDAGESDPSVTRSITIASCGCLNPPANALVCAGDDQNLVGNT
                     SSMLVGTCSVPPEAQKCEYTCAPGFVDQGGVCTVIRPLDSLTCSVTPPYPYTHTTHVT
                     WTVNPLPLGSYTYLWSGSVSAVTSINSTTTIYYRSGTKTERVTVFADPAQMYQIGDVQ
                     CPNLNVTLHPGYGEG"
     gene            9683..10876
                     /gene="nusA"
                     /locus_tag="VI981_02655"
     CDS             9683..10876
                     /gene="nusA"
                     /locus_tag="VI981_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY4479230.1"
                     /translation="MFDLKVINSVLKQLEEERGIPREKIVEAIEMALATAYKKEYGKK
                     GQIVRAKFNPDTGKTEFFQVKIVVDETRVIMEEELAEYEAKKTAEQEIEDDRARFNGE
                     HHILIDGAKKIKKDALLDDEIIFALEPKEDYGRIAAQTAKQVIIQKIREAEKVSVLDE
                     YGKREGEIVSGTVQRIERGNIYVDLGRTNGILPYEEQIPGEHYKQGERIRAYLFSVEE
                     SPKGIFLRLSRSHPKFLEELFKIEAPEVASGTVEIKAIAREAGSRSKVAVMSNDPHID
                     PVGSMVGQRGVRVSTVMSELGGEKIDIIEWSVDPKKFIEDALSPAKVSSVEINESETK
                     ATVKVAEDQQSLAIGKGGQNVRLAAKLTNWRIDIQSEKGQIVDETESAPSDSKPEDSA
                     DKKNE"
     gene            11156..13144
                     /locus_tag="VI981_02660"
     CDS             11156..13144
                     /locus_tag="VI981_02660"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY4479231.1"
                     /translation="MIIFSLGSASVAIVAAFILGLRILKRSPGNEKMVEIAKAIQEGA
                     RAYLNRQYKTIAIVALVVALLIGFFINWTTMAAFLVGAIASAIAGYIGMHISVRANVR
                     TAEAARESLRSALTVAFHGGSVTGLLVVGLALLSVTLFFMITRDVTALIGLAFGGSLI
                     SVFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAGMAADL
                     FETYAVTAIAAILLGSLTFPGSESILLFPLAIGGIAALASVLGIFFVRLGKKERIMGA
                     LYKGVAASSILSLIGFWILIKQTDIAPLIDMTDLTVFAALITGIFVTALIVVITEYYT
                     SKSFRPVRSIARSSETGHGTNIITGLSVGKEATALPVIVIVAGILASYSFGGLYGIAL
                     AAMSMLSMAGIIVAVDAFGPITDNAGGIAEMAGLPKEVRVITDALDSVGNTTKAVTKG
                     YAIGSAGLAAVVLFASFVEDLRKAGINEQFDLSNPKVIVGLMIGGMIPYLFASISMKA
                     VGKAASSVVFEVRRQFKEIPGIMAGTARPDYARCVDIVTRAAIREMIIPALLPVIIPI
                     LVAVLLGPSALGGLLVGSIVTGIFVAISMTSGGAAWDNAKKYIEEGYHGGKGSEAHKA
                     AVTGDTVGDPYKDTAGPAINPVIKIITIVALLLIPFLG"
BASE COUNT         3612 a         2982 c         3016 g         3665 t
ORIGIN      
        1 cgatgtccac gacttcaact tcagttgaat tgccatcaag cgagatcact tcagtagcat
       61 ttgacgcata atttctttta atccgatatg caaggtcttc aattccggac tctgcgagtg
      121 cataactttt gcctgacaaa agtagtgttc ctcctcttgc taaatgttcg ttgatcgtgg
      181 aagcaagacc gagaataacg atgagtgaga cggatgtaac aacgagaact gacacgagca
      241 tgacctgtcc tcgtttccat tgattcgaat tttttttaaa acggtaaatc attttaattt
      301 tgatacgagc ctcgtataac agccgtgcca aagaagtgct ctttcttgac cctctgtctg
      361 acagttgtct cgagttgagt ttcgatcttt actccttctg atcttcccgt cgaaatccgt
      421 cggaatatga gggaaagcgc ctttgtcccc gtcgccatca gtgatacacc tacgcccgtg
      481 ccctcctttg ccatgatagc gtcattttga agataaaact ctatagtggt agcggctcca
      541 gaagaatcga ccgtgttcaa cttgagcgtt ccgggagtcg cgtcaagaac acttgagcct
      601 gtatctatac tcgcggcatt tcgaatttca agcatcattc gaccaatagt attatcagca
      661 tttaccgcca agtctttgtt taatttaata ggtccggagg agttggttat cgagacgatg
      721 aggtaacaca tgagcgtaat aacgagcgcg gtaataccca cgtagataag catctcaaca
      781 atagcgaatc cttttttttg tattagggac ttcatgatca ttaattagca aaaaggtccg
      841 tgagataaaa agacgtttgt tttgtggttg tggcattgcc atttttccag gcgacctgca
      901 tggttatctt ttttgtacca gaatcaagcg caccgccaga acttacgata tctttagtcg
      961 tatcatctcg gttcaccgac gagatcgtaa aggagcgctc gtagcgttca gttataaatg
     1021 gtacagcctt tcctacagtt ggtatctgca tctgccatcg tgcggttgag ctcgcccacg
     1081 acaaataaaa agttccggat gttgatgttc cgacggtatt tacccaggat tgatctcgaa
     1141 gtgagcgtac agcctcaatt ccttcatcaa tcaatagtcc gccttttaca aaagggagtg
     1201 tgtatctcga cgctttaaga aataaggtaa gcacatcatg tagtgagaga acggtgagaa
     1261 ggaaaattga cgtcccgaca aggacttcta caagtagtga acctttttgt ttcttatgaa
     1321 aaacctgcat gacacatatt agagcataac actcctaaaa gttaattttt ctcgatgact
     1381 ccagtttgag atatagtgat tttttttgtt ttgctcgtat cactccgaag cgatacagtg
     1441 atcgtcccgg tcgcctgtgt ttcgccggtc aagcgctcga ataaaacttc agaaccgccg
     1501 ccgaggagac tgatcgaatt tatttgtgaa taagagtcga gcgtgtaaat tctatttgat
     1561 ggttcagtcg acgaatacgt atttttatag agcacaactt gataggtatc aaaatgcacg
     1621 ccgtaacgag cgctattttc ggaggcaaga gtctttcttc ttgcctcttc caaatatgag
     1681 gccacagtgt gagccgtctg atctacggcc tgatgcttcc tgtacgaaaa gagaccggtg
     1741 ataatgatag cactcatgaa accaagaaca acgaccgata tgagcacttc taccagggtc
     1801 attccttttt ttttaatcat attgcgttca ataaggaata cgtcggctgg atcatggcaa
     1861 cagcaaaaaa tcctacggaa gcaccgatga taatcataag aaacggctcg atcactgtcg
     1921 acatatcttt agttctctgt tcgacttcat tctcataaaa gagggccaca tccgcaagca
     1981 tttcagccag tttcccagtt tcttcgccga cattgatcat ttctcccaca aaagccgggt
     2041 agagatgttc attcttttta aacgtacttg aaatcggttc gcctttctgt attttttctc
     2101 gcgcttcttc cagaactttt ttatgatgca gattttggat gacgtctctt gtaatttcga
     2161 tcgctcgtaa ataatcgact ccagacgaaa gaagtgatga gagtgtccgc gcagtttgag
     2221 ccgcatttga ttctttgatg atccctccga caatcggaat atggagtaat ataagttgca
     2281 gaaatttttt ggtgccttgt ttcttgttaa aaaaatacaa gccgccgaaa aaactaatga
     2341 agaataccac gagtaacaga taatgatttt ttacgaagtc acttgcccca atgatgaaga
     2401 gggtcgtaat gggcaaggta acgtccatgt ccttgaagac actcgttaag gtcggcacca
     2461 catacacaag tagtacgatg cttatgatga ccatgagaat gagaatgata gacgggtaga
     2521 tcatggcccc ttttatcctt ttatggagtt tataaacttg atccatctga tctgaaagtc
     2581 tcttcagaga tgcagcgagc gtcccactct cttctcccgc gcggatcata gccgaaaaaa
     2641 tatccggaaa aatccgagga tattttttca atgcctctga aaaagactta cctgctttca
     2701 cttcattatt gagctcgccg ataataagct taaatctcct gtttcggctc tgtcgttcgc
     2761 ttatggtcaa agctcgagac atcggcagcc cggcttcaag catcgaggcc atattgcggc
     2821 tgaatactat tttctcctgc tgtttaatcg tactcacatc agcgagccgt ttccaaagtg
     2881 attttttcac tgccgtctcg gttacactta cgactgtctc gccttctttt cttatatcgc
     2941 gatagagcga atacttgtcg atcgcgtcgc gcgtatcttc acgaattgtt ccgtctgctt
     3001 tttttatttt ataaataaat gttggcatat tattcactcg aaacagtccg caacacttct
     3061 tctatagtcg taatcccttc tgccgctttg aacagtccgt cttccagcat tgtcatcatc
     3121 ccctcctctc gagcttgttt ttctatggtg tcagcagttt ctaacttcac gattaagtcg
     3181 cgaattgtgt tcgtaacttt caaaacttcg cttataacca cacgcccttt ataaccgtcc
     3241 tcgctatcac gcgaaggttt tgacttgtaa aagggaatct tatcccaatc cagattagca
     3301 ccgataattt gctctttttt cagtaccgat aatacaagat caagattggc atgtttccct
     3361 attgattcta tttccgcaga agacagaaaa tgttgctgtt tgatttctgc caatcgccgc
     3421 acgagccgtt gagcaatcgc aacattaacc gttgacgcaa caagaaaggg ctcggccttc
     3481 atatcggcaa gacgcggaaa agaagttgcc gctgaactcg tatgcaaagt cgaaagcaca
     3541 aggtgtcccg taagcgcagc gttgatggca agaccggctg tttccacatc ccgaatttct
     3601 ccaaccatga tgatatcggg gtcttgcctc acaagcgtac ggaggccgct cgcaaaagtg
     3661 aagccgattt ctggcttgac ttgtgtctgg tttattctct tcatttgata ctcgattgga
     3721 tcttctacag tagaaatgtt cacatcggga gtattcaata tatccatcat ggtgtagagc
     3781 gttgtagttt taccggaacc ggtgggacca gtggtcaata tgagaccgct cgacgcctta
     3841 agtgcctgat gaagactttc gagtccttca ccatgaaatc caagtttttc aagtgtaaaa
     3901 ccggcagcat tttcgcggag aactcggagt acagcttttt cgccatgata tgtgggaatt
     3961 aaagaaacac ggtacgacac attttcacca gagaggtctt gagcaaaccg gccgtcttgc
     4021 ggtaaacgtt tctcatcgag cttcatttgg gccagcactt tcactcgggc aataatactg
     4081 ctcgccgatt ccttcggtag aatcatagcg tcatgaagta atccgtctat ccggtatctg
     4141 atcaagagtg actcgtcagt aggttcgata tggatgtcgg aagcattttg caaaatcgcg
     4201 tgcttcagca aggcgtcgac aatcccaata accggaagct cttcggccat ttttttgaga
     4261 tcggtttccc cggttgcaac actcattttg gccgtaaggg cttttacctt ttcggcttcc
     4321 ttttggatga tgtcgccaaa ttctgccttc aaactttttt gatattgaag cagtgctgat
     4381 tttatagaat ccgaatcggt gagacgagct aaaatcctta acttgacctt cttttttatg
     4441 aagtcgattg ctgcaagatc atcggtatcg gtcatcgcca cttcaagtgt gtcgctagag
     4501 cgtttaaaag caacgatatt gtggctacgg gcaataggtt ccgggataag cgacagtatc
     4561 tcccggtcga gatgctcgtt ttttagatca acaaacggga ctcccagtat gtaaccctgc
     4621 atttttcgaa gatcatcttc agaaagttta ccttgtgaca caagtacgct gccgacactt
     4681 gttttctttc ctacggcttc ttcgtaggcg gcaagtacgt cttgttttga gacaagtccc
     4741 gaatcaagta gaaaatcttt taattgtttt tcttcaatgt acatcccgtt agaaatagga
     4801 aacgctcaac aaaccttgag aaatagcgtt cgtatcaaaa ttatagccta aatcaggtaa
     4861 taattgctgg ttaatcgtga aggcgtttcc gttaatgact ccaatagtcg aattggcata
     4921 tgtatgttct gcgtaatcac agtttcaaaa tacataactt tttgatttct aacgggatac
     4981 atgccaattt ttgtgaacaa aaatgaacta aaattgaaca ctcgctatgt aaaatccgat
     5041 cgctaactta cgaaacgatc atttcgtctg cctattcgtg atgactcgta gtttgtacta
     5101 ttgtatcaac aaaaaactta cccctgtgtg ggtaagtttt tgttggaaag agtctccgat
     5161 tatccttcgc cgtatccggg atgaagagtc acattaaggt taggacactg cacgtcacct
     5221 atttgataca tttgcgcggg atcagcgaac acagtcaccc gttccgtttt agtgccagat
     5281 ctgtaatata tggtcgttgt tgagtttatt gaagtcacgg cgctaactga accgctccag
     5341 agatacgtgt atgaaccgag aggaagcggg ttgacagtcc atgtaacgtg agtcgtatgg
     5401 gtatacggat atggcggagt tactgaacac gtcagcgagt cgagaggacg tatgacggtg
     5461 cagacgccac cctgatcgac gaaaccggga gcacatgtat attcacattt ttgcgcctct
     5521 ggaggaacgc tacatgtccc tactaacata ctcgaggtat tgcccaccaa attttggtca
     5581 tctccagcgc acacgagtgc gttggctggg gggttgaggc atccgcagct cgctatagtt
     5641 attgatcttg ttacggatgg gtcagattct ccggcatcgt taaccgctgt tattctgtaa
     5701 tagtacataa ctcccgcagt cagtccgctc tcgagatatt gcgccgctat agtacccgcg
     5761 cgcaacgcaa aatttattcc gtttgtgctt cgatatacat tgtagccagt tgagtctggt
     5821 acaggatccc atcggacaac aacagaaccg cacgtagctc caggatcgac ggtcacccct
     5881 ccgggaggag aagggactcc gtaggtaaag aagacttttg tcacggtatt gcctaagact
     5941 actacgtttg cggagcatac agtggtagtg caagtgactc ccgtcgtgct gtaacttgtt
     6001 attgggcact ctacccctcc gaccggatag gtacagcttc cgactcgttc gacaaagtta
     6061 ggaagattcg tgacgttcgc agaatgccca ccaattgaaa tatcggtatc gtgagcagga
     6121 tttgcggctg acgttggtat tccgtcaaga ccccacgatg tccttccaaa aggcatcatc
     6181 aaagctaggt tgttatcaag tcgtgcatta agtacttgcc cgagaatagg gccgacatcc
     6241 cgaacatatc taaacgccac tttgcggacc cgatttgccg ccaccggctc gttgacactg
     6301 cagaaccctt gagagcaggt caaacctgca gtttgatact gcgatacgtc tgtcatgcgg
     6361 cattcagttg ctccttccgc atagtcgcac gtattccact gctctgtgta tccaggaagg
     6421 tcagtcacaa aaattgtgtg atttccaaga ctaatgtttg ttctagtttc agggtttacg
     6481 gttgaaatga cagtaccgtc tattccccac gacgtagtgt ttgggttcag agccgaaggc
     6541 agagtactga aattttcatc cactcgaact gtccgaatac ttccggtcgg aactacaatt
     6601 cgaacatatc taaatgccac tttcgttacc gcatctacgg ccacgggtat tgaagcagag
     6661 cagaaacctg tagcgcacgt gacaccaacc gatgagtacg ttgatgggtt tgtcatgaag
     6721 cactccggac cggaacttgg atagttgcag acgcttatct cttccgtata tccagcaaga
     6781 tcgctcaccg aaattatgcg cgtaccactc ggaactccag aacgtaattc tggattgaca
     6841 gaactcgacg gcccatagac ggcaccgccg tcaagcatcc acaaagtgtt attaagattc
     6901 gcagcacttg gtaaaacatt gaaagtttga tcaacacgaa tactctttac agagccggta
     6961 gttgaacatg cgcctgtcgc cacgccggag actacacttg agagcggtga ttcgatcgag
     7021 ccgtcaacgg tcgaaactgc atagtagtat gtcattccca aagtaaggcc tgtatctgtg
     7081 taagaagtaa cgataacagg agtagaattt atcttcgtaa atgacatagg aatgcccgag
     7141 agagaacggt agacattata tgaagtcgct ccaccaacac tattccatga aagatttatt
     7201 gctccattac atggggccgg tgtcgcatta actcttaaat tccccggggc accgacgtat
     7261 ttaaatgcca ctttaatagt gttatttgga ggcacagtta tattggcact gcatgcggcg
     7321 ccagcacaat cgaggccgag gttagttgtt gaatattgaa cgtcggtatt tacaaaacac
     7381 tccgctggag caaaaagaca tgaaccaacg ccaacggtga agcccgaaag cttcgttgcg
     7441 ttaattgtgt gtgagcccga aaggacaact attgtactac cgtgattttg tgccactcct
     7501 ccatctatat tccatgtggt tccaggcatg gcacctaaga gtccgtctac tagcatccct
     7561 ttgacagagc cgttttgagt agccgtattt acgataaggg tgaaagttgt tgtcttggtt
     7621 ggtgaattgc ctgaaacaac aataggatac gagccgcttg gagtgctcgc aagtgtcgtt
     7681 atagtaagcg aacttacaca cgagggattg ggtgtgcaag gattattatc aaatactgcg
     7741 gtggccccag ccggcaaacc tgatacggta agtgtcacag gctgagccat gcccgtaatt
     7801 aacgttaggg taatggtatt tgagccactc aaaccttgtg tcaccgtaat ggtgccgcta
     7861 ttttgaagtc cgtagtcaaa agatggtggc gctgtaacta tgacagaatt gtcacaaaca
     7921 attgtagttg atgccgttcc tgaagtgacg gtaaccgagc ccgttttagt acccgccgct
     7981 gtgtatgatt taactacaga tgagctaacg cccgaaagta aatccgtgcc gctccatgag
     8041 tacgtatata ttcctgtgcc accagtggcc gtggacgtcc acgtaacatt ttggccagtg
     8101 tctgcagaag ttggcgcaac gctacaagtt ccggtaacag gtaccgtaac gacagcgctc
     8161 gcaaccggtg agacgccgcc tgggccagca cagttcatta aaaaatccgt ggtaacagta
     8221 agcggaccgc ttgattgatt accatttata gctttaaccc cattccaatt cgaattagtc
     8281 ggtaaagcgg ttgctgtgca agagttcgaa tttgttgagc tccacgtaag cgtagacgcg
     8341 ctacccgatg gtacagaagt ggggttggcg cttagagtca cggtcggagc aggtacggga
     8401 ggaattgtag ccaatgttat tactccagcg acaaaactca cacaatcgcc accagtatca
     8461 tcgtagccat gagtaagatt taccggctgt gtcgcaatat ctaacatggg cgatatcgac
     8521 cagcttcgaa tatcccaaag atttccgtta tcaataggtc cgttgtttgg cccaggtacg
     8581 gttctgcctt gaaaaattga tccccccgcc atgagcgtcg agccattgat tttaacggca
     8641 gggtccgggt acgtctgtcc gtcagccacg tgtagctcaa gtgtgccatt gccatccgga
     8701 gcattgacat caggaatgct tatagcccag ccttccgcgt catacgcact aactttatta
     8761 gaatcgttcc ctgttgttaa aatgtaatta ttattgttgg cggcgttacc atcgtcaaag
     8821 aagactagga gcgatgcccc gtttggattg aggttacctt tgtttgaaaa agcgcttata
     8881 atatacctcc catcacctga aacggtacca gttacatcag ccatgaaact gtgggattta
     8941 aaccatggtc caatgggagc agtatatgtg tcccagcaat taccatcaga cacacctgtc
     9001 tggattcctg taaccctttg actaattgca gttccagaat gagcaatggt caccagttga
     9061 gaagtagtat tacctacaga accattcgag acactattcc aaataagaaa agcttttgtg
     9121 acagtaccgc ttacgccagt aagattaata cctacgcttg tagcattgcg caatcctcca
     9181 acaccagcac tcttgtaatc aagattggaa aatctctgat gattggttac tgcagatgcc
     9241 tcgactgtct ctgtaggatg aaatattcct acaatcgatg gggtgacaat gaataaaaca
     9301 aataaaaatt taaatagctt ctttgaatga tgaatacgca taattgagac taattatata
     9361 acagaagcac cactccgagt gtttataacc aaaagctttg ttgcaagaac tgatacaaga
     9421 gctaacagat ggttttaaat gaaactggat gaacctcatt attggaaatt gtcatttatt
     9481 ttgacatcta tagcaacata tactattgtg tagaggtaag ttcgcctcac ggcggactcg
     9541 ttttttaaat gccaatctgc gaatggatac caatcatact aatgaagaaa cagatttcgt
     9601 cattagtatg aattcttaat atcgatatta tctattcgta taattggtac aaattagtat
     9661 attagtataa tgcgaagcaa tcatgtttga tctaaaagta atcaattcgg ttctaaaaca
     9721 gcttgaggaa gagcgcggta ttccgcgcga gaaaatcgtg gaagccatcg aaatggctct
     9781 tgccactgcg tataaaaagg agtatggcaa gaagggacag attgtccgtg ccaagtttaa
     9841 tcctgatact ggaaaaaccg agtttttcca agttaaaatc gtcgttgacg aaacgcgggt
     9901 gattatggaa gaagaacttg cggagtatga agccaaaaaa actgcagaac aagaaatcga
     9961 ggatgacaga gcccgtttta acggagaaca ccatatccta attgacggcg ccaagaaaat
    10021 aaaaaaggat gctttgcttg acgatgaaat cattttcgcg ctcgaaccaa aagaagacta
    10081 cggaagaatc gctgcccaaa ccgcaaaaca agtcattatt cagaagatac gcgaggccga
    10141 aaaagtttca gttcttgacg aatatggcaa gcgcgaagga gaaattgtct cgggaacggt
    10201 tcagcgaatt gaacggggca atatctatgt agacctcggc agaacgaatg gtattttgcc
    10261 ttatgaagaa caaattcccg gtgaacacta caaacaaggg gagcgaattc gagcctatct
    10321 cttttcagta gaggaaagtc cgaagggaat tttcctaaga ctctcccgct cacatccaaa
    10381 gtttcttgaa gaattattca agatagaagc gccagaagtc gcgagtggca cggtagaaat
    10441 taaggccatt gcgcgagaag caggttcccg ttccaaggtc gctgtcatgt ccaatgatcc
    10501 ccacattgac cccgtcggat cgatggtcgg tcagcgaggc gtgcgagttt cgactgttat
    10561 gagcgaactt ggtggagaaa aaatagatat tattgagtgg tcagtcgatc cgaaaaaatt
    10621 tatagaagat gcgctgtctc ccgcaaaagt ttcgagcgta gaaatcaatg aaagcgaaac
    10681 gaaggcaact gtaaaagtcg ccgaggacca gcagtcgctt gcaatcggca aaggaggcca
    10741 aaacgtgcgg ttagcggcta aactaactaa ctggagaatc gatattcagt ctgaaaaggg
    10801 tcaaatagtt gacgagactg aaagtgctcc ttccgattct aaacccgaag actcggctga
    10861 taaaaaaaac gaatagtcgg cggagacgga attcacacga tgatggaccc tgaagtgagc
    10921 tctggcctta cgtaagggta gacagactgg catttgtaag tataatcctg aagtgagttc
    10981 tttaatttga taaatatttc aaatgtgtcc ccgcttgact atattttttg cttgcgaata
    11041 ggaagcaaaa gtgaagtgta ttgaaatatt tcattcattg tgcctttcag gcacaatcaa
    11101 attaaagctc tacttcagga tataatcaca aaaaattaca aataattcta taatcatgat
    11161 tattttttcc ttaggaagtg catctgtggc tattgttgca gcgttcattc ttggacttcg
    11221 gattctaaag agaagtccgg gaaatgaaaa aatggttgag attgcgaaag ccattcaaga
    11281 aggcgcgcga gcatatttga accgtcaata taaaacgatc gctattgttg cgctcgtcgt
    11341 cgccctcctt attggtttct ttataaattg gacaaccatg gctgcatttt tggttggtgc
    11401 tatcgcttct gctatagcag gctacattgg tatgcatatt tctgttcgcg ccaatgtccg
    11461 caccgccgag gcagcaagag agagtctccg ttcggccctc actgttgcat ttcacggagg
    11521 aagcgtcaca ggacttttag tcgtcggtct tgcgcttctt tcagtgactc tcttttttat
    11581 gataactcgt gacgttaccg cactcattgg tctcgctttc ggcggaagcc ttatttcggt
    11641 tttcgctcga ctaggaggag gaatttttac taaaggcgct gatgtcggag ctgatcttgt
    11701 aggaaaagtt gaagctggaa ttccagaaga tgatccgcgc aaccctgcag tcatcgccga
    11761 caacgttgga gacaacgtcg gcgattgcgc aggaatggca gccgaccttt tcgagacata
    11821 tgcagtaacc gccattgcag ctattctgct tggttcccta accttcccgg gttccgagag
    11881 catcttactc ttcccgctcg caatcggcgg tatagcagca cttgcctctg tacttggaat
    11941 attttttgtt cggctcggaa aaaaggaaag gatcatgggc gcattgtaca aaggagtggc
    12001 cgcttcgagt attctctcac tcattggttt ttggattttg attaaacaga ctgacatcgc
    12061 cccgcttatt gatatgactg atctcaccgt attcgccgcg ctcattacgg gaattttcgt
    12121 gacggcgctc attgtcgtaa ttacggaata ttacacttca aaatcgttca gaccagtgcg
    12181 ttcaatcgca cgttcatcgg aaactggtca cggaacgaac atcattacag ggctttctgt
    12241 cggaaaagaa gccacggcac ttcccgtaat cgtcatcgtt gcaggtatct tagcgtcgta
    12301 ttctttcgga ggactatacg gaatagcact cgcagcaatg agtatgcttt caatggctgg
    12361 cattatcgta gctgttgatg ctttcggtcc aatcacggat aacgcggggg gaatcgccga
    12421 gatggccggg ttgccgaaag aagtcagagt cattactgac gcacttgatt cagtcggaaa
    12481 cacgacaaaa gccgtcacaa agggctatgc catagggtca gcaggactgg cggctgtagt
    12541 gctctttgcc tctttcgtcg aagacttgag aaaagccgga ataaatgaac aatttgattt
    12601 aagcaaccct aaggtcatag tcggccttat gatcggtgga atgatcccct atctttttgc
    12661 ctcaatttca atgaaagcgg tgggaaaggc ggcaagtagt gttgtctttg aagttcgaag
    12721 acaattcaaa gaaattcctg gcatcatggc gggaacagcg cgtcctgact acgctcgctg
    12781 tgtagacatc gtaactcgcg cagctatacg agaaatgatc atccccgcat tacttccggt
    12841 aatcattcct atactcgtcg ccgtacttct tggaccttcg gccctcggcg gtttacttgt
    12901 cggttccatt gtcacaggta ttttcgtcgc tatttctatg acttccggag gtgcggcgtg
    12961 ggacaacgca aaaaaataca ttgaagaggg ctatcatggc ggcaaagggt cagaggcgca
    13021 caaagccgca gttaccggag atacagtcgg cgatccttac aaggacacgg caggtcccgc
    13081 cataaacccg gtcatcaaga tcatcactat agttgcgctc ctccttattc cattcctcgg
    13141 ataaccgaga caaaaaatat agtttgaagt cgagctccat tctgtaagaa agcttatgta
    13201 tatgcgtcta ttttgcatta agaaacgcgg gctgtagata gggggatcag atgtataatt
    13261 taatatgaaa tttat
//
LOCUS       DATDSU010000081         6327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2238344, whole genome shotgun sequence.
ACCESSION   DATDSU010000081 DATDSU010000000
VERSION     DATDSU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238344"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1314
                     /gene="ruvB"
                     /locus_tag="VI981_02665"
     CDS             289..1314
                     /gene="ruvB"
                     /locus_tag="VI981_02665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY4479232.1"
                     /translation="MTDVKTKEKAEAAGEEVYLDQTLRPSLWDDYIGQKNIKENLRIL
                     LSAARERKHPPEHILFYGPPGLGKTTLAHLIAKEQNAQMKHTSGPAIEKVGDLASILT
                     NLSAGDILFIDEIHRLNKSIEEVLYPAMESGVLDIIIGKGPSARTIQLELPPFTLIAA
                     TTRVAQLSAPLRSRFSGGVFRLEFYAQSEIEEIIKRSAKILGVEMEKGASIEIAKRSR
                     FTPRTANYLLKRTRDFAQVRKEKLTEALVLEALTLLGVDMLGLAQSDRHILDTIISKF
                     GGGPVGLNTIAAALSEEQATIEEFNEPYLIQMGLLDRTPRGRVVTEHGYRHMGKSVPA
                     NLQEKLI"
     gene            complement(1349..1963)
                     /locus_tag="VI981_02670"
     CDS             complement(1349..1963)
                     /locus_tag="VI981_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479233.1"
                     /translation="MVNQELLNYIKQQTVAGVSREIIVANLLSQGWSQADVDAAFLQI
                     NANASAVPLPQIAQTALLEKKHDVLWVVLNIVLRGGIVGGPIFVLCFIVAGIVTINLG
                     FDAQKIAAYPELKIYFFIGYQLFELIIYPAAYLGIRWGCKYVAKNTFIEEKDFGRIGR
                     YVGLIPAVFSIARSFSIAAWLYGVFLTPNLVKKWLKKYSTEVVV"
     gene            2106..2927
                     /locus_tag="VI981_02675"
     CDS             2106..2927
                     /locus_tag="VI981_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY4479234.1"
                     /translation="MSKIINIPRGQLADLYLAQSFSSYQIAKQLGCSQNTIMERLRVF
                     KIKTRSIQEGKALTKPRYPRKNFYGNKQDKSYLIGFRLGDLYVRKAHLNSPTIQINLN
                     STRSEQVELMKNLFAPFGHVGISRPDKAGATKVRAYVNCSFEFLVPKNKALDSWILKS
                     KKNSIAFLAGYVDAEGSFGLSPRNQPFFSMKSQDKEIMKSIQARILPSVGIDTKLQFV
                     RSAGSIMNNIRCNKDVYGIFIYNKQHLGLLLSALLPLLKHSKRRVDALKVFNRIK"
     gene            2927..3460
                     /locus_tag="VI981_02680"
     CDS             2927..3460
                     /locus_tag="VI981_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4479235.1"
                     /translation="MSGHNRWTQIKRQKGKTDAEKSKVFGKFSRLITEEAKKTAGNLS
                     APSLVAAIDRAKAANMPNDVIDRAVKKATLDKSAALEAILYESYGPGGCGIIISTLTS
                     NKNKAAQEVKFILGKHGSSLAGMGSVTWGFERQRDGTWKPTTMTPLSDEDAEKLGALV
                     EELESNDEVQEVFTNAE"
     gene            3568..4053
                     /locus_tag="VI981_02685"
     CDS             3568..4053
                     /locus_tag="VI981_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY4479236.1"
                     /translation="MRILAIDPGYERIGIAILDKQQPRDILVYSECFKTSARDAFEKR
                     LCDIGLKISSIIDAFSPEVLAIETLFLNTNQKTAMRVSEARGVIIYEAAKRTLRVEEF
                     TPLEIKVATLGYGRGDKKSIMSMVPKLVTIKKAIKHDDEFDAIAAGITCSAHNRIGRL
                     R"
     gene            4203..4355
                     /locus_tag="VI981_02690"
     CDS             4203..4355
                     /locus_tag="VI981_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479237.1"
                     /translation="MAQKHDNDFLDKEKEGEEGIVDDGTEDSNPSVSPDTSDDEEEDF
                     ESRWKE"
     gene            complement(4483..5904)
                     /gene="tyrS"
                     /locus_tag="VI981_02695"
     CDS             complement(4483..5904)
                     /gene="tyrS"
                     /locus_tag="VI981_02695"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY4479238.1"
                     /translation="MAEEKVKDSAVEIETLLRDGVVDFVDPGETLKKKLLEKARGTYT
                     KDIIIKLGADPNRPDIHLGHAVILRKLRKFQDAGCKVVFVVGDYTASIGDPSGKNKVR
                     VEVAEKEIQENMNTYVAQVGKLLKTDPSVFSWIRNSDWFYGITDLIFDEKTKVGIDFR
                     QGAETKHVPVEPNSFIGKALLYENTRMQKTSLGRKETATITLKGLLWTLRHITHARLI
                     ERDMFRVRIQKGEELYMHEMLYPVLQGIDSSVISLIYGSCDLEIGGTDQTFNMLIGRD
                     VLRANKQPEQAVMTMPLLLGTDGKEKMSKSLDNYIGITDEPNNMYGKIMSIPDALIVS
                     YFRLCTYETSGQIAEIEGAMTQGKNPKDLKMKLAREIVALYHGEKKSHEAETNFTNTF
                     SKGGVSEDALAVEVSKGTALSDVALEHELVSSKSEWRRLIDEGAVRNMETDQKIENYD
                     FKIDTGLVVKIGKRRFLKIGIKT"
     gene            6022..>6327
                     /locus_tag="VI981_02700"
     CDS             6022..>6327
                     /locus_tag="VI981_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479239.1"
                     /translation="MHFFLRFLKKAKNRHMLKQGVIVCISLSGIVAGLLILWLATFRL
                     PDLNAFEERKLTQSTKLYDRTGKVLLYDVHEDIKRTVVLYENISRYVKNATVAIEDAE
                     "
BASE COUNT         1840 a         1397 c         1346 g         1744 t
ORIGIN      
        1 tcaaccttga aacaggcaag gcttgcgtgc tttcaaggct gacagttcaa tttccgaagt
       61 tgaaccattt ctctcgatac gtaagtcaaa ctccacttca ggttcaataa ctcacgcttt
      121 attacagcca caagaaaagg cgtttgtgcg cccttttgtt tatatgtatt tttcaattat
      181 gcaccgcgta ccgtccggcc tggtatagat agacactagc ataaattata tttaatgtca
      241 agtagcttgt cgcggcctgc cccgatcctt tttgctacaa tagaagtcat gacggacgtc
      301 aaaaccaaag aaaaagccga ggcggcgggc gaggaagtgt atcttgacca aacacttcga
      361 ccttcgcttt gggatgacta tataggacag aaaaatataa aagagaatct ccgtattctt
      421 ctctctgccg cccgtgagcg taaacatcct ccggaacaca ttctcttcta cggaccccca
      481 ggactcggga agacgactct cgctcattta atagccaaag aacagaacgc gcagatgaag
      541 catacttcgg gaccggctat cgagaaagtt ggcgacttag cgtctattct caccaatctt
      601 tcagctggag atattttatt tatcgacgaa attcaccgtt tgaataaatc tatcgaagag
      661 gtgctctacc ctgccatgga gtccggtgta ctcgacatta ttatcggcaa aggaccgtcg
      721 gcacgcacca ttcagcttga attaccgccc tttactttga tagcggccac aacacgagtc
      781 gcacaactct ccgctccgct tcgttcacga ttttccggcg gtgtttttag actcgaattt
      841 tacgcgcaaa gcgagattga agaaattatc aaacggtcgg caaaaatact cggggttgaa
      901 atggaaaaag gtgcttcgat tgaaattgca aagcgtagcc gattcactcc acgaacggca
      961 aactatcttt taaagcgtac gcgagacttt gcacaagtcc gcaaagaaaa gttgacggag
     1021 gcgcttgttc ttgaagcgct aacattgctc ggcgtggaca tgctcggtct tgcacagtca
     1081 gaccgtcata tactcgacac aattatttca aagtttggag gaggtcctgt tgggctcaac
     1141 actattgcgg cggccctttc tgaagaacag gccacaattg aagaattcaa cgagccatat
     1201 cttatacaaa tgggactttt ggaccgcact ccacgaggac gcgtcgtcac ggaacacggt
     1261 taccgacata tgggaaaatc tgtaccggcg aatttacagg aaaagttaat ttaggttgta
     1321 gggagtaggg ggtagtgagt agggggaatt aaacaactac ctctgtagaa tatttcttaa
     1381 gccatttttt taccaaatta ggagtaagaa atactccgta aagccacgcg gcaatactaa
     1441 atgatctcgc aatactgaac actgcaggaa ttaatccaac ataacgtccg attcttccaa
     1501 aatctttttc ttctataaat gtattcttgg caacatattt acacccccat ctaataccga
     1561 ggtacgcggc gggataaatg attagttcaa ataattgata tccgataaaa aaataaattt
     1621 ttagttccgg ataagcggcg attttttgag catcaaaacc taggttgatc gttacgatac
     1681 ccgctactat gaagcaaaga acaaaaatag ggccacctac tattccgcca cgaaggacaa
     1741 tgttcaatac gacccacaac acatcgtgtt ttttctcaag cagagctgtt tgggcaattt
     1801 gaggcagagg gacagcagat gcattcgcat tgatttgtaa aaaagccgca tctacgtccg
     1861 cttgagacca accctgagaa agaaggtttg cgactataat ttctcgagaa actcctgcta
     1921 cggtctgttg tttgatgtaa ttaagcaatt cctgattaac catgagaata gtttatagtg
     1981 ataatgacat aagtttaccc cccccatata gaataaaagg gaaacaactc gtcgtggtaa
     2041 atttgaactc ttttcttttt tgactgacta tgtttgcgaa gatattaatg tggtacactt
     2101 agtctgtgtc caaaataata aacatcccgc gtggtcaatt ggcggattta tatcttgcgc
     2161 aatcttttag ttcctaccag attgctaaac aactgggatg cagccaaaat acaattatgg
     2221 aacggcttcg ggtttttaag ataaagactc gaagtataca agaaggtaaa gccctaacaa
     2281 aaccacgata tccacgaaag aatttttacg gtaataagca agacaaatct tacctaatcg
     2341 ggttccgttt gggagacctg tatgtgagaa aggcacatct aaatagtccg acaatccaaa
     2401 taaatttaaa tagcactagg agtgaacagg tcgagttgat gaaaaactta tttgctccat
     2461 tcgggcatgt gggaataagt agacctgaca aagcaggtgc aacaaaagtt agggcttacg
     2521 taaattgttc ttttgaattc ttggtgccta aaaataaggc attggattca tggattttga
     2581 aatcaaagaa aaactcaata gccttcttgg cgggatatgt ggacgctgaa ggctccttcg
     2641 gtttaagtcc gcgaaatcag ccgttctttt cgatgaaaag ccaagataaa gaaattatga
     2701 aatcaattca ggctcgtatt cttccatcag tgggaataga cacaaaatta cagtttgtga
     2761 gatctgcagg ctctattatg aacaatattc gatgtaataa agacgtttat ggaattttta
     2821 tatacaataa acaacatcta ggactgttac tttcggcact tttaccccta ctgaaacact
     2881 ccaaacgtcg ggttgacgca ttaaaagtat ttaatcgaat aaaataatgt ctggacataa
     2941 tcgttggaca cagataaagc gccaaaaggg gaaaactgat gctgaaaaga gtaaagtttt
     3001 tggcaagttt tcgagactca tcaccgagga ggccaaaaaa accgccggta atttaagtgc
     3061 gccgtctctc gtagcggcca ttgatcgggc aaaggcagcc aacatgccaa acgatgtaat
     3121 agaccgtgcg gtcaaaaaag cgacgttaga taaatcggcc gcattggaag cgattttata
     3181 cgaatcatac ggcccgggtg gctgcggcat aataatttcg acacttactt caaacaaaaa
     3241 caaagccgcc caggaggtaa aatttatctt ggggaaacac ggttcgtcac tcgcaggtat
     3301 gggctccgtc acatgggggt ttgaaagaca gagggatggc acatggaagc cgacaaccat
     3361 gactccgctg tctgacgaag atgcagaaaa attaggggct ctcgtcgagg aacttgagag
     3421 taacgacgag gttcaagaag tttttaccaa tgctgaatag aaaagaaggg tctagggttt
     3481 agcgataagg gtttagtaac ccggggacat ttcccacaaa aatattcttg tattgcacta
     3541 tacgctaaac gctgtaccct atacgctatg agaatcctcg ccattgatcc tggatacgag
     3601 cgcatcggca ttgctattct cgacaaacag cagccgagag atattcttgt ttattcagaa
     3661 tgtttcaaaa catccgcccg cgatgcattt gagaagagac tttgcgacat aggtttaaaa
     3721 atctcctcaa tcatagatgc gttttctccc gaagtgctcg cgatcgaaac tctttttctc
     3781 aataccaatc aaaaaacggc catgcgggta tctgaagcaa gaggtgtcat tatttacgaa
     3841 gcggccaagc gtactctacg cgtggaggaa tttacaccgc ttgagataaa agttgcaacg
     3901 cttggctacg gtcggggtga taaaaaaagc atcatgagca tggtcccgaa gctcgttacc
     3961 ataaaaaagg ccattaagca cgatgacgag tttgacgcca ttgccgcggg catcacatgt
     4021 tcggctcaca atcgcattgg ccgcttgcgg taaaaaatgc tttagcatat acggcaactg
     4081 tccgttgaaa tttatctctg gttttccaca tgtttttaga tttgcaaaat ataaaagatt
     4141 tgataaaaat atggggtcat gcggtccgta gaccgtaaaa attaaaaata agggaatttt
     4201 ttatggcaca aaaacacgac aacgatttct tggacaagga gaaggaaggg gaagagggca
     4261 tagtcgacga cggcacagaa gactcgaacc cgagtgtttc acctgatacg agcgacgatg
     4321 aagaagagga cttcgagagc cggtggaagg aataaagggc actcgcgtat tcctttttaa
     4381 atgcacgaaa atcttttggt aaagattttc tctgtccggc gccgtcgcgc cttccaaggc
     4441 atttaaaaag gaacgcactc gccgagacga aaggataact ttttatgttt taattccaat
     4501 tttaagaaat ctgcgtttgc cgattttcac cacaagccct gtgtcgattt taaaatcata
     4561 attttctatt ttttgatccg tttccatatt tctcaccgct ccttcatcga ttagccttcg
     4621 ccattcagat tttgatgaaa caagttcatg ttcgagcgct acgtcagaaa gggccgtccc
     4681 tttcgacacc tcaaccgcaa gagcgtcttc cgaaactccc cctttagaaa aggtattcgt
     4741 aaagtttgtc tccgcctcgt gtgatttttt ttctccgtga tagagcgcca ctatctcacg
     4801 tgccagtttc atttttaagt ccttcgggtt tttcccctgc gtcatggccc cttctatttc
     4861 ggcaatctga ccactcgttt cgtaggtgca tagtcgaaaa tacgacacaa taagtgcgtc
     4921 cggaatggac attattttac cgtacatatt gtttggttcg tcagtaatcc caatatagtt
     4981 atcgagactc ttcgacattt tttccttccc gtcggtgccg aggagaagcg gcatggtcat
     5041 gacggcttgt tcgggctgtt tattggcgcg taggacgtct ctaccgatta gcatgttgaa
     5101 tgtttgatct gtaccaccta tttcaaggtc gcaactcccg tagataagcg agatgacact
     5161 tgaatcaatt ccctggagaa ctgggtagag catttcatgc atataaagtt cttcgccttt
     5221 ttgaatacgt accctgaaca tatcccgctc gatcaaacga gcatgggtta tgtgccgcaa
     5281 agtccacaga agccccttca gggttatggt cgcagtttct tttctgccaa ggctcgtttt
     5341 ttgcattcta gtattttcat acagcagtgc ctttccaata aacgagtttg gctccacggg
     5401 aacatgcttc gtctcggcac cttggcgaaa atcaatgccc acttttgtct tttcatcaaa
     5461 tataagatcc gtgattccat agaaccaatc ggaatttcta atccaggaaa aaacggacgg
     5521 gtcagtcttc agcagtttcc cgacttgtgc gacatatgta ttcatatttt cttgtatttc
     5581 tttctctgca acctcgacgc gcactttatt ttttcctgac ggatcgccga tactggccgt
     5641 gtaatctccg acaacaaaaa cgactttgca tcctgcgtcc tgaaatttcc gaagctttct
     5701 caaaatcacc gcgtggccga gatgaatgtc cggtcgatta ggatcggctc cgagcttgat
     5761 gatgatgtct tttgtatacg ttccacgagc tttctcgagt aatttctttt ttagtgtctc
     5821 tcctggatcg acaaaatcaa cgaccccatc tctaagcaag gtctctattt ctaccgcaga
     5881 gtctttgact ttttcttcag ccatgtcaaa tactctacca taaagttcgg tatttttccg
     5941 aaaaacactc ataagatttg gttttctcaa tcgtcatcaa caaacaattt atttgtttcg
     6001 attaacagat ataatacaag gatgcacttt tttcttcgtt ttttgaaaaa ggcaaaaaac
     6061 cgccacatgc tcaaacaggg ggttattgtc tgtatttccc tttcgggcat tgtcgccggc
     6121 ctcctcattt tgtggcttgc aactttcagg ttacctgact taaacgcctt cgaagaacgc
     6181 aagcttaccc aatcgaccaa actatacgat cgcactggca aggtgcttct ctatgacgtc
     6241 cacgaggata tcaaaagaac tgtagtgcta tatgaaaata tttcccgcta tgtaaaaaat
     6301 gcgactgtcg ccattgaaga cgccgaa
//
LOCUS       DATDSU010000082        11383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_743018, whole genome shotgun sequence.
ACCESSION   DATDSU010000082 DATDSU010000000
VERSION     DATDSU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743018"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VI981_02705"
     CDS             <1..621
                     /locus_tag="VI981_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4479240.1"
                     /translation="DEFPEFERRVIDALRQPLEDRVISISRARGSAQFPADFILIATL
                     NPCPCGNFGTKGKECICTASNLIRYQRKISGPIIDRIDMWIEVPNVEHYKLSDDKSKG
                     ENSEKIRERVLAARKLQEHRFSKMKKEIKTNSRMSSRDLEKHVPLSNEVKDILNTSAA
                     KLGLSARAYHRVIKLARTIADLDKKEHIETKHIYEALTYRPKKIGA"
     gene            complement(629..1429)
                     /locus_tag="VI981_02710"
     CDS             complement(629..1429)
                     /locus_tag="VI981_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4479241.1"
                     /translation="MHQNIFETLGLSLNEGKIYETLLERGETTISAMAVAAKIHRRNA
                     YDVMQRLVNKGLCFQIFSTTENRYNAVDPDKLTEILAEKQAKLMEVLPALKKKFGERF
                     APEEAYIYRGFEGQKNIWRDVIRVGQESFFIGAKAGWFDPRLETSRIAFFKEANRKKI
                     KFIQLFDFEVKAMVPNFPAHFPGSLTYRFLPKKYSTNSAIHIFGDYVITYTGLTIGKI
                     DEDVVFFVIHSKGLAESYRTWFWYMWEQSTEDKSPNKKSPAGSGRQEL"
     gene            1542..2321
                     /locus_tag="VI981_02715"
     CDS             1542..2321
                     /locus_tag="VI981_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479242.1"
                     /translation="MKVTMSLIAGTLGLSLSVTSAADKTNCWSPNGSWSLSLQERYIA
                     IRVSRVPHNGLSLWSDLNINLPEGFYVNIWDHHGIEGRDKSKQPDELDLAFGWRRQIS
                     WYDLEVGLATSYLNNSPLGLWLTHDALSQAATISKTYAFGQHTLKPQIRVEWMSLATD
                     FDGGSFILMPNVSHVWRQPFSTKPLCFTHQLFMVHDDGFVGPKNDSEGYFLRWNAGLR
                     WQLSKRMTLTLPSYAAQIPLHKGNDGRGQATSWGTMLTWSF"
     gene            2360..3301
                     /locus_tag="VI981_02720"
     CDS             2360..3301
                     /locus_tag="VI981_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479243.1"
                     /translation="MKAEINRRKNTPEMKKIGEKFMVRRKRLKISQTVVAEWLGVSQR
                     HVSYFEEGKARLSSRQIVTLNDKLAATEKGAPTQIPPKPLNLLSAIDFLQIITETKES
                     DVADVWLFGPEKSAIFLDRALKDSWTACVDTGATLHVVWRLDVKMEKYAMRLFSGVAA
                     RIICDLTRSSSPPSEIGSIDIYAIRGANQGYGGNAESYRDLQDHFKQELFSGRVKVHD
                     PVDISSEKFDRFHSLLDLGRFGPVLAYSGKSNTPGVLVSPIAVGVMIEHVSLKPHTCA
                     DISFEGMREIAESGWVFLATDDMYHLEYRCAEFLKAF"
     gene            complement(3625..4266)
                     /locus_tag="VI981_02725"
     CDS             complement(3625..4266)
                     /locus_tag="VI981_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4479244.1"
                     /translation="MNIKHAIYRTALAWSEQRLGKRLDPIKRELFGNLSGTVLEIGPA
                     TGANLAYFPERISYVAVERFFGLRQILEDKLVHFKFEKAATFHGRAEAMPFERNSVDF
                     VVATFVLCTVQDQREILTEVFRVLKHGGHFVFIEHVGASHGTVTHALQRLVKPFTRFI
                     AYGCEPDRDTIDNIRWVGFRPVSYNTLYVPRLALMPIVYGSAHKFGPLEAKHK"
     gene            complement(4371..4994)
                     /locus_tag="VI981_02730"
     CDS             complement(4371..4994)
                     /locus_tag="VI981_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4479245.1"
                     /translation="MRTKRLRLKTDTSDDVHVDLARRGDTEAFEKLYVKHASNLRAAA
                     LRLTGSHHMADEVCQIAWIKAWKNLPRFKGHSSFNTWLNVIMRYGFYSIIEKRNCLRI
                     IDESQLMPDSEDGGLAELAIDLEPTPAEMCARVETARLVRGAIRNLPDFCRDVARLHL
                     EGHEHHRIAKTLDVAIGTSLSRLHNAKRRLATVLADRLNGEPADQFL"
     gene            complement(5092..6069)
                     /locus_tag="VI981_02735"
     CDS             complement(5092..6069)
                     /locus_tag="VI981_02735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4479246.1"
                     /translation="MVVVVFSFVADVNAGFFSFLADLVSDESKAAPREDRGLNSQTMA
                     LLQAEVGSLPTGGGDTDPTIVDDSAIASSIGPSGTALDMEEKPISHEISVYVVRKGDN
                     LSSIAKMFGVSVNTILWANDMRASTLKEGQQLVILPVTGVKYKIKTGDTLKSIATRYK
                     ADIDEIVAYNELDPLAKLEVGLEIMIPDAEIVIPKATPSYSTPLRGGGGPTLAGYYMR
                     PTTGRKTQGLHGYNGVDLASPAGSPIVASAAGTVIIVKTGGWNGGYGNYIVVKHGNGT
                     QTLYAHNNRNLVSVGDYVSQGQSIGTVGSTGKSTGSHLHFEVRGAANPF"
     gene            6641..8593
                     /locus_tag="VI981_02740"
     CDS             6641..8593
                     /locus_tag="VI981_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY4479247.1"
                     /translation="MQEHLESLNTQQKEAVLHVNSPLLILAGAGAGKTKTITHRILHL
                     IKSGIPPQEIMAVTFTNKAAKEMRDRVMSLIKKDKELNSPILFTETPFVSTFHSLGVH
                     ILKENARLLGLTRHFTIFDRSDSLSAVREAVRETSLDPKQYDPGKFLRAISREKGDMI
                     TVEEYATGARKEYWGKLVISVWQKYEAALRKENALDFDDLLLKTALLLKDHKEVLGKY
                     QSVWKYIHIDEYQDTNRVQYMIAKMLSAKERHICVVGDIDQNIYSWRGADIRNILEFE
                     KDYPDARTVLLEENYRSTQTILETANKVIAKNKKRREKNLFTRAGWGEKIALFEGYDE
                     IDEAHFTATKSKELIAAGTNPNEISLLYRANFQSRALEEAFLAHGVPYQVLGVKFFER
                     KEVKDVLSYLRVALNPQSLSDMKRVVNVPPRGIGKVTLLKIFSGAESNLPVSMKDKLG
                     KFRILLSEIRVDIEKEKASTVLKNLVVESGLESLYKNGTEEDIERLENLYELVTLASK
                     YDLMTAPLGIEKLLEDAALATDQDEMEEEKKGVKLMTVHAAKGLEFDIVFITGLEENL
                     FPHKNLSEESMTEDKAEEERRLFYVALTRARKKLFLTLASVRTIFGSKRLNVPSEFLS
                     DIDEKLIEAVTPPPEGPRREKIIWFD"
     gene            8720..9376
                     /locus_tag="VI981_02745"
     CDS             8720..9376
                     /locus_tag="VI981_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY4479248.1"
                     /translation="MRRVKIATGEHYHVYNRGVDKRTIFSDGRDIDRFMKSVREFNTR
                     TPIGSIHENRYGQAKNQPELQEPLVEFVAYCVLPNHYHFILKQVIDEGIEKFMHRLSV
                     GYSMYFNKKHTRSGSLFQGTYKAQHIDSNEYLLHLSAYINLNNHVHKKWGGNLNFVRS
                     SWKEYLNPMNSDITKKDIILSQYPNSESYQSFAFEALNNIRDRRELSQIVSIDNDLET
                     "
     gene            9431..10204
                     /gene="rsmA"
                     /locus_tag="VI981_02750"
     CDS             9431..10204
                     /gene="rsmA"
                     /locus_tag="VI981_02750"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY4479249.1"
                     /translation="MFQKKSLGQNFLKNPGIISDIVAASKVSPDDIVLEIGPGQGILT
                     RELLIKTKKVIAVEKDDRLISYLQERFHSEIQAGKFEIMHADILDFDLDSALGDKPYK
                     IVANIPYYITGQVIRKFLETNNQPVSMTLLLQKEVAERLVAKDGKESLLSISVKVYGK
                     PKYVRTVARGNFRPIPKVDSAILSVENITKSLFSDVDRDKFFEILKKGFAHKRKLLAS
                     NLEIPKAKRSFFFKAIDINEQTRPEDLTVVEWKKVVSHL"
     gene            complement(10199..10636)
                     /locus_tag="VI981_02755"
     CDS             complement(10199..10636)
                     /locus_tag="VI981_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479250.1"
                     /translation="MKYISIKLTTLIKKELIAMLIAAGVITLSLIYVKNSKFSVLHAQ
                     AQDQTSGASSNTSGSLPFGGQVSVAFYCCEGISYGVVEPVGGEFLWDYSATLYREFMA
                     FEAHDQWMIGNYRPGGVCSDIESECEDSRPMTGTIDMIFGTSL"
     gene            complement(10633..>11383)
                     /locus_tag="VI981_02760"
     CDS             complement(10633..>11383)
                     /locus_tag="VI981_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEY4479251.1"
                     /translation="AGSYTIAKLRTLQKTTGSGQATINGTPYFSLGDILPVGFTPDLQ
                     FDSHVSTIMSVYKDFKEKLRVLYPSSYFGSYNQTLTMYGTGFANSGNVLHFGNNDKIE
                     NLSSPDHVRLSFTIPLGIRAGKHNIWVSNSTGQSNQEQFFIVTPINAEAPRIDAVTPK
                     EGPNGTRVEITGSGFLPVNEIRTSYGTYTVESADRRNISFTVDFHIFDKLQKLRDRGN
                     EKVTATKIPVTLQVMNENGLSDSILFYITLR"
BASE COUNT         3304 a         2629 c         2529 g         2921 t
ORIGIN      
        1 gacgaatttc ctgaatttga aaggcgcgtt atcgatgcac tccgacaacc gcttgaagat
       61 cgagtcatca gtatttcgcg agcacgcggt tccgctcaat ttcctgctga ctttatttta
      121 atagcgacac tgaacccttg cccctgcgga aatttcggaa cgaagggcaa ggagtgcatc
      181 tgtaccgcat cgaatcttat tcgatatcag aggaaaattt caggtccaat tatcgatcgt
      241 attgatatgt ggattgaggt gccgaatgtt gagcactata agctttcaga tgacaaatca
      301 aaaggtgaaa attcggaaaa gatccgcgag cgtgttttgg ccgctcggaa actgcaggag
      361 catcggttct caaaaatgaa aaaagagata aaaacaaata gccgcatgag ttcacgcgat
      421 cttgaaaaac acgttccact ttcaaatgaa gtaaaggata ttctaaatac ctcggccgca
      481 aaattgggac tttccgcccg cgcctatcac cgcgtaataa aactggcgcg taccatcgca
      541 gacctagaca aaaaggaaca tatcgagacg aaacatattt acgaagccct cacataccga
      601 ccaaagaaaa ttggagcgtg attatcactt atagctcttg cctgccagaa ccggcggggc
      661 tttttttatt gggagatttg tcttccgtcg attgctccca catataccaa aaccatgtcc
      721 ggtagctttc cgccaagcct ttgctgtgaa tcacaaaaaa aacaacgtct tcatcgattt
      781 tgccgatggt gagacctgta taagtaatta cgtaatcgcc aaaaatgtga atcgctgaat
      841 ttgtggaata tttcttcggt agaaatcggt atgtcagact accagggaag tgggcaggga
      901 aatttggcac catcgcttta acctcaaaat caaacagctg aatgaatttt attttctttc
      961 tgttagcttc tttaaaaaaa gctatccgac ttgtttcaag gcgcggatcg aaccagccag
     1021 ctttcgcacc gataaaaaaa ctctcctgtc ccacacgaat cacgtcgcgc caaatatttt
     1081 tttgaccttc gaagccacga tagatgtacg cctcctcagg cgcaaaacgc tcgccgaact
     1141 tttttttgag agctggaaga acttccatta attttgcctg tttttccgcc agtatttccg
     1201 tgagcttgtc agggtcgacc gcattatatc gattttcggt agtcgagaat atctgaaaac
     1261 aaagaccctt gttcaccagg cgttgcatca catcataagc gttacgacga tgaatttttg
     1321 ccgctaccgc catcgcactt atcgtggttt cccccctctc aaggagagtc tcgtaaattt
     1381 tgccctcatt caggcttagt cccaatgtct caaatatatt ttggtgcatc gaattaagta
     1441 aatacttgtg gtgtaagcta tcaccacatg gtggtattgg ccactcaact gtagtttata
     1501 taataccttt cggatagtca accttaacaa ggaaataaca aatgaaagtc acgatgagcc
     1561 taattgcagg cacactaggt ctgtccctct ctgtcacaag tgcggcagat aagacgaatt
     1621 gttggtcacc gaatggatcg tggtctctgt ctctacaaga acggtatatc gcgatcagag
     1681 tctccagagt ccctcacaat ggcctgtcgc tttggtcgga tctaaacatc aacctaccgg
     1741 aaggttttta cgtgaacatc tgggatcacc atggaattga gggccgggat aaatccaagc
     1801 aaccggacga acttgaccta gcgttcggct ggcgcagaca aatatcttgg tacgaccttg
     1861 aagttggact cgcgacgtct tatttaaaca actctccgct tggcttgtgg ctgacgcacg
     1921 atgctctgtc tcaagcagca acgatatcga agacttacgc tttcggtcag catacgctta
     1981 agccacagat tcgagtcgaa tggatgtcgc tcgcgacaga tttcgatgga ggctctttta
     2041 tcctcatgcc gaatgtttct cacgtctgga ggcaaccctt tagcaccaaa ccgctctgct
     2101 tcacccacca actatttatg gttcatgacg acggtttcgt cgggccaaaa aatgattcgg
     2161 aaggatattt cctccgctgg aacgcgggct tgcgttggca gttgagcaaa agaatgacgc
     2221 tgactctgcc aagctacgct gcccaaattc cactacacaa gggcaacgac gggaggggcc
     2281 aagcgacttc ctggggtacg atgctgacct ggtcgttctg aaacaatcaa ccgtgtaaaa
     2341 ggaaactaaa gaaagttcta tgaaagccga gataaatcgc agaaaaaaca cacccgagat
     2401 gaaaaagatc ggagaaaaat tcatggtccg ccgcaagcgc ctgaaaatat cacaaactgt
     2461 tgtggcagag tggctcggtg tctcccaacg tcatgtcagt tacttcgagg aaggtaaagc
     2521 gcgtctttca tcacggcaaa tcgtaactct gaacgataaa ctggcagcaa cggaaaaagg
     2581 tgcgcccaca caaataccac cgaagcctct taacctattg tcggcaatcg attttctgca
     2641 gatcataacc gaaacaaaag agagcgacgt cgcagatgtc tggctgttcg gaccggagaa
     2701 gtcggcaatt ttccttgata gggccctgaa ggacagctgg acagcttgcg ttgacacagg
     2761 cgcaactctt catgtcgtat ggagacttga tgtcaaaatg gaaaagtatg ccatgcgact
     2821 gttcagcggg gttgctgccc gaatcatctg cgacctaaca aggtcatcat cacctccatc
     2881 tgaaatcggc tccatcgaca tatatgcgat taggggagcc aatcaaggat atgggggcaa
     2941 tgctgaatct taccgagatc tgcaggatca cttcaaacaa gaattattca gcggccgcgt
     3001 caaagttcac gaccccgtcg acatcagttc cgaaaagttc gaccggttcc attctttgct
     3061 ggacttggga aggttcgggc ctgtcctcgc ctatagcggc aagtcgaaca caccaggcgt
     3121 actggtttct cccattgccg tcggagttat gattgaacat gtatctctta aaccgcacac
     3181 ctgtgccgat ataagctttg aaggcatgcg ggagattgct gaatcaggct gggtctttct
     3241 ggccactgat gacatgtatc atctggaata tcgctgtgca gagttcctaa aagcattttg
     3301 aagtaagacc ttctcatgca aatccgtcag tcgcatactg gcggattttt tcttttgcaa
     3361 accgaccatc aaagaggtca gcttgaaaaa aggcagcatt ttttgcggta cgaacaaatt
     3421 ggtgatcgac ttctaaaaaa caatgaagct ccccgcagca agctgtcccc tcactccgct
     3481 tcgcttcgta tcggcggggt atctaggagc ttcgctctgg attatcttga aaaccaaggt
     3541 tttcaaactt tcctttacgg ctccccgcag tagagctgac ggggtatata taaaataaaa
     3601 aaagcgacat tgctgtcgcg cgactcattt atgctttgcc tctaatgggc caaacttgtg
     3661 tgcactgccg tagacaatgg gcataagcgc gagccgcggc acgtaaagag tgttgtagct
     3721 cacgggcctg aacccgaccc atcttatatt atcgattgtg tcccggtcag gctcgcaacc
     3781 gtacgcaata aagcgcgtaa agggtttaac aagcctctgc agtgcgtgag tcactgttcc
     3841 atgagaagca ccgacatgtt cgatgaatac gaagtggccg ccatgcttca acactcgaaa
     3901 cacttcagtc agaatctccc gttgatcctg taccgtgcag agtacaaaag tagcgaccac
     3961 aaagtccacg ctgtttctct cgaagggcat cgcttccgct cttccgtgga atgtcgcggc
     4021 tttctcgaat ttgaaatgta cgagtttgtc ctcgagaatt tgtcttaacc caaagaagcg
     4081 ttcgactgcg acgtatgaaa tacgctctgg aaaatacgca agatttgctc ccgtggccgg
     4141 accaatttct aagactgtcc ccgacagatt cccaaaaagc tcacgtttaa tcggatcgag
     4201 tcgtttaccg agcctctgct cgctccacgc gagtgcagtt ctgtagattg catgttttat
     4261 gttcatcccg caaaacatta tcagaagatc gaaaaaaaga aaatgacgtc cttcccggac
     4321 ctgcgtgcat gtttttacgg gtaagaaaaa acctgcatca cgatgcaagt tcataaaaat
     4381 tggtccgcag gttcgccgtt aaggcggtct gcgaggaccg ttgccagccg ccttttagcg
     4441 ttatggaggc gcgagagcga agtgccgatc gcaacgtcaa gagttttcgc aattcgatgg
     4501 tgctcgtgtc cttcaagatg cagtctcgca acgtcgcggc agaaatcagg cagattacgg
     4561 attgcccctc gcactagacg tgcagtctca actcgagcgc acatctccgc cggtgtaggt
     4621 tcaagatcaa tcgcaagttc agcgaggccg ccgtcttcag aatcgggcat cagttggctc
     4681 tcgtcgataa tgcgcaagca atttctcttt tcgatgatgc tgtagaatcc ataacgcata
     4741 attacgttga gccacgtatt gaacgagctg tgccccttaa atcgagggag gtttttccac
     4801 gccttaatcc aggcaatttg gcaaacctca tctgccatat ggtgcgaccc cgtcagtctt
     4861 aaggcagccg cgcgtaaatt agaggcatgc ttgacgtaga gtttctcgaa agcctctgtg
     4921 tctcctcgtc gagcaagatc aacatgaaca tcgtctgacg tgtctgtctt caacctaagt
     4981 cttttggtcc tcatacagtt tgtccgggag taattcgatc ataaacgcac aggaattgca
     5041 acaaaaacac ctgaaagtag gcttagtcgt taaaacagtg tcaaaatggt tttagaaagg
     5101 atttgccgcc ccacgaacct caaaatgaag atgcgatccg gtcgatttgc cggtactgcc
     5161 tacagtaccg attgattgac cttgactgac atagtcgcca acactcacaa gatttcgatt
     5221 attgtgggca tacagcgttt gagtgccatt accgtgtttg acaacaatat agttaccata
     5281 accgccgttc catcctccgg tcttaacaat gataaccgtg cctgccgcgg aggcaacaat
     5341 aggacttcct gcaggtgaag cgagatcgac accgttgtat ccgtgtaaac cttgagtctt
     5401 acgtcctgtc gttggacgca tataatatcc agcaagggta ggccctccgc ctccgcgaag
     5461 cggtgtcgag tagctcggag ttgcttttgg aattacgatt tcagcgtcag gaatcataat
     5521 ctcaagacca acttcaagct ttgcgagggg atcgagctca ttgtaagcca ctatctcatc
     5581 gatatcagct ttgtaccttg ttgcgattga ttttaaagtg tctcccgtct taattttgta
     5641 tttaacaccg gtaactggga gaataaccag ttgctgacct tccttcagag tactcgcccg
     5701 catatcattt gcccacaata tcgtgttaac actgacccca aacattttcg ctatagaaga
     5761 gaggttgtcg ccttttcgaa caacatagac gctgatttca tgtgatatcg gtttctcttc
     5821 catatcaagc gcagtgcctg aaggtccgat cgatgaggca atggctgaat catcgacaat
     5881 agttgggtct gtgtctccac cacctgtcgg aagagaaccc acttcggctt gaagcagagc
     5941 catagtctgt gaattaagac ctctgtcttc ccttggagcg gccttacttt catcgcttac
     6001 aagatcagcc aaaaaagaaa aaaaaccggc atttacgtcg gctacaaaag aaaaaacgac
     6061 tacaaccacg agtacattcg tgcaaaataa cttgaaaaat aaggatgtga tagccgtacg
     6121 cctcccaaca aagttcggag caggctcctt ttgatttaga ttaatataag taaagttaag
     6181 ctacatatac gagattttca gtaaaaatgg cttatctaag ccatgccaag agatttgtga
     6241 gccagcggtt cagaatatgt aacgccatct actatagccc atccagagct cgaattgaag
     6301 tgccctgtct gcatgttcga acgaatgtgc tgcgagccgc cgcacgcttt agagccaaaa
     6361 aaacggctac tattccaaaa tagccttatt tttaggtatt taataagttg tggaaatccg
     6421 gtcaaaaaaa cgctattacg aaacaagttt ccactcatgt ctacaaaaat ttgctacttt
     6481 gcagacttat tggattattt gtgaagaatt tcgactctca agcatctcaa aactaaacgc
     6541 cttctctttg ttacaaatct ttgttacaaa aaacttcaga gagcctaccg tcatcgtctg
     6601 ttttttattc ttacgatatc caacatgcta tagtgagcga gtgcaggaac atcttgaatc
     6661 actaaataca cagcaaaaag aagccgtttt acatgtaaac agcccgcttc ttatactggc
     6721 tggggccggc gccggtaaaa caaaaacaat aacgcacaga attcttcatc tcatcaaatc
     6781 gggtattccg ccgcaggaaa tcatggcagt cacgttcacc aacaaggcgg cgaaagaaat
     6841 gcgcgaccga gtaatgtcgc taatcaaaaa ggataaagag ttgaatagtc caattctgtt
     6901 caccgaaacg ccatttgttt cgacttttca ctcgcttgga gttcacattt tgaaggaaaa
     6961 tgcgagactt ttaggcctga cgcggcattt taccatcttc gaccgctccg attcactctc
     7021 cgcagtgcga gaggccgtca gagaaaccag cttagaccca aaacagtatg acccggggaa
     7081 atttttgcga gccatctcaa gagaaaaagg agacatgata acggtagagg aatatgcgac
     7141 aggagcccgc aaagaatatt gggggaaact tgttatttcg gtgtggcaga aatatgaagc
     7201 ggctctgcga aaggagaatg ccctcgactt tgatgacctc ttactcaaaa cggcactgct
     7261 cctaaaagac cacaaagaag ttctagggaa ataccagtct gtctggaaat acatccacat
     7321 agacgaatac caggacacaa atcgagtcca gtatatgatt gcaaaaatgc tttcggcaaa
     7381 ggagcggcac atttgcgtcg tgggggatat cgaccaaaat atttatagct ggcgaggcgc
     7441 ggacatacgc aatattcttg aatttgaaaa ggattaccca gacgcacgca ccgttttact
     7501 cgaagaaaat taccgatcaa cgcagacaat tctcgagact gcaaacaagg ttatcgccaa
     7561 aaataaaaaa cggcgggaga aaaatctctt tactcgggcc ggttggggag aaaaaatcgc
     7621 gctctttgaa gggtacgatg aaatcgatga ggctcatttt acagcaacaa aatcaaaaga
     7681 attgatagcc gcaggcacga atcccaatga aatcagccta ctctaccgcg ctaactttca
     7741 gtcgcgcgca cttgaggagg catttcttgc ccacggcgtg ccgtatcaag ttcttggtgt
     7801 gaaatttttt gagcggaaag aagtgaagga tgttctttca tatctgcgcg tcgcgcttaa
     7861 tccgcagtcg ctgtcggaca tgaaacgtgt cgtaaatgtg ccacctcggg gaatcggcaa
     7921 agtgacactc cttaagattt tttctggagc agaaagtaat ctgccagtat caatgaagga
     7981 caagctcgga aaattcagaa tcctgctttc tgaaatcaga gttgatatcg agaaagaaaa
     8041 agcgtcaacg gtactcaaaa acttagtggt ggagagcggt cttgaatccc tctataaaaa
     8101 cggcacagag gaagacattg aacggctcga aaacctttac gaactcgtga cgcttgcatc
     8161 taagtacgac ctcatgacag ctcccctggg aatagagaaa ttacttgagg atgcggcact
     8221 cgccaccgat caagacgaaa tggaagaaga gaaaaaagga gtaaaactca tgacagttca
     8281 cgccgctaaa ggtcttgaat ttgatattgt ttttataacg ggacttgaag aaaacttatt
     8341 tcctcacaaa aatttgagtg aggaaagtat gaccgaagac aaggccgagg aagaacggag
     8401 gcttttttat gttgccctga cacgcgcccg aaaaaaactc tttctgacgc tcgcttcagt
     8461 acgcactatt ttcggaagta agcgtcttaa tgttccctct gaatttttaa gcgatataga
     8521 cgagaagcta attgaagcgg tcacgccgcc accggaagga ccgcgtaggg aaaaaattat
     8581 ctggttcgac tgacgtaaga tatctgctga agtccctcct cctcctcaac ttggagactt
     8641 agtctccaag ttgaaaaaac aatataaatc gtaaagttat ccacttggag acttagtctc
     8701 caagtgttaa tgtctttata tgagaagggt aaaaatcgcc acgggcgaac actatcatgt
     8761 ctacaatcgc ggagtagata agcgcacgat tttttccgac ggccgagata tcgatcgttt
     8821 tatgaaaagc gtgcgagaat tcaacaccag gactccaata ggtagtattc acgaaaacag
     8881 atatggtcaa gccaaaaacc aaccggagtt gcaagaaccg ctcgttgaat tcgtcgcata
     8941 ttgtgtcttg ccaaatcact atcactttat actgaaacag gtcattgatg agggtattga
     9001 aaaatttatg cataggcttt ccgttgggta ctccatgtac ttcaacaaaa aacacacaag
     9061 atcaggctct ctctttcagg gaacatataa ggcgcaacat attgacagca atgagtattt
     9121 gctacaccta agtgcatata ttaacctcaa caatcacgtg cataaaaaat ggggcggcaa
     9181 tttgaatttt gtccggtcga gttggaagga gtacttaaat cccatgaata gtgatatcac
     9241 aaaaaaggat attattttaa gtcagtatcc gaattcggaa tcatatcaat cgttcgcatt
     9301 cgaggccctg aacaatatcc gcgaccgtcg agagctttct caaattgtgt cgattgacaa
     9361 cgacttggag acttagtctc caagcggggt ctcaaagtgg gataggggag gaaaatttat
     9421 aattataatt atgtttcaga aaaaatcgct cgggcaaaat tttctaaaaa atcccggcat
     9481 catttcagac atagttgccg caagcaaggt atcgccagac gacatcgtgc ttgaaattgg
     9541 accgggacag ggaattttga caagagaact cctcataaaa acgaagaaag tcatcgccgt
     9601 tgagaaagat gatcggctta tttcctatct acaagaaaga ttccattcgg aaattcaagc
     9661 gggaaaattc gagattatgc atgctgatat cttggatttt gacttagatt cggcgctcgg
     9721 agacaaaccc tacaagattg tcgccaacat cccatattac ataactggcc aagtcatccg
     9781 gaaatttctc gaaacaaaca atcaacccgt atcaatgaca ctgcttcttc aaaaagaagt
     9841 ggccgagcgg ctcgtcgcca aggatggaaa ggaaagtctt ctctcaattt cagtaaaagt
     9901 ttatggcaaa ccaaaatatg tacggacagt agcccgaggg aattttagac cgattccaaa
     9961 agttgattca gctattctct ccgtagaaaa tatcactaaa agtctttttt ccgatgttga
    10021 tagagataaa tttttcgaaa ttttaaaaaa aggttttgcc cataaacgca aactgcttgc
    10081 ttcaaattta gaaattccaa aagcaaaacg atcgttcttt ttcaaagcga tagatattaa
    10141 cgaacaaaca cgaccggaag atctcacagt tgtggagtgg aaaaaagttg tttctcacct
    10201 atagggacgt cccgaaaatc atgtcaatag tgccagtcat cggtcgtgaa tcttcacatt
    10261 cggattctat gtcggaacaa acaccaccgg ggcgataatt tcctatcatc cactgatcgt
    10321 gggcttcaaa agccatgaat tctcgataca gcgtagctga gtaatcccag agaaactctc
    10381 ctccgacggg ctcgacaaca ccgtaactta ttccctcaca acagtagaac gcaaccgaaa
    10441 cctgacctcc aaaaggaagc gatccggacg tattcgacga ggcgccggaa gtttgatctt
    10501 gcgcctgtgc atgcaaaaca ctaaattttg aattttttac ataaatgagg gacaaagtga
    10561 tcactcctgc ggcgattaac atcgcaatca attctttttt tataagcgtt gttaatttta
    10621 tggaaatata tttcatcgaa gtgttatgta aaataaaatc gaatcggata aaccattttc
    10681 gttcatgacc tgtagagtaa ctgggatttt tgttgcagta actttttcat ttcctctgtc
    10741 tctcaacttt tgtaatttgt cgaaaatgtg aaaatcaacc gtgaaagaaa tattcctgcg
    10801 gtctgcggat tcaacggtat acgtgccgta acttgttctg atttcattga ctggcaaaaa
    10861 accggatccc gtaatctcta cccgcgtgcc attcggtcct tctttcggag tgaccgcatc
    10921 aatgcgcggt gcctctgcgt taatcggcgt tacaataaaa aattgttctt gattgctttg
    10981 acccgtcgag tttgagaccc agatgttgtg tttgccggca cgaatgccga gtggaattgt
    11041 gaatgaaagc ctgacatggt caggggagct taaattctca atcttgtcat tatttccgaa
    11101 atgaaggacg tttccagaat ttgcaaatcc cgttccatac attgtgagtg tttgattgta
    11161 tgagccgaaa tatgacgaag ggtagagtac tcgaagtttt tctttaaaat ccttgtacac
    11221 gctcataatc gtcgacacat gggagtcaaa ttgcagatcg ggtgtaaatc cgaccggtaa
    11281 gatatcgccg agcgaaaaat atggagtgcc gttgatcgtc gcttgccccg accctgtcgt
    11341 cttttgaagt gtccgtaact tagcaatcgt atacgatccg gca
//
LOCUS       DATDSU010000083         4528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_78626, whole genome shotgun sequence.
ACCESSION   DATDSU010000083 DATDSU010000000
VERSION     DATDSU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78626"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..531
                     /locus_tag="VI981_02765"
     CDS             76..531
                     /locus_tag="VI981_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479252.1"
                     /translation="MHKNWKVICQIPDTHVMWALALLDMDSDLGRTFVGRFPEYFTQE
                     EQAGPVPLSVLRQEAAENAVHQHGGTAGDNHLVSYNAIPAWMPQLYFAAGAALEEYKE
                     QAMPVPSELEKVKVVGTVCGWKGKKLVLVDYLYDGQWWFVPAELIAVDR"
     gene            complement(901..1575)
                     /locus_tag="VI981_02770"
     CDS             complement(901..1575)
                     /locus_tag="VI981_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479253.1"
                     /translation="MSEKERFTPEQERRVITLEDIMDSSRQVPEEYKQLILAQEQRQG
                     DWYLGTRFVDQWHGFDEFPHARDLLKQRLAGDILVDLGGRTGSFSLAHHQFGVTTYIN
                     VDRDYGDYPVNPLAALNEGDIGDLHKLSVKADMLDFVARMRDDSANFMLNGIDFASVP
                     GSERYHEVLAQELVRATKPGGLVFGINATVDHIWREMSDKAPLRDQSLDLPGVIHDSF
                     VYQKPE"
     gene            complement(1979..2494)
                     /locus_tag="VI981_02775"
     CDS             complement(1979..2494)
                     /locus_tag="VI981_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479254.1"
                     /translation="MSKRDDFDRNTIEVIGKRASFVCSNPDCKSLTLCPSEENEDKFI
                     YIGKAAHITAAAEGGPRYDSNLTSEQRKSTNNGIFLCSNCADMIDKNNGIDFSVDQLK
                     QWKKEHEEWATQNLNKSLNSLITTIDGEHTARGKGNVTGIKTTRPTFFKPGTKSTAEG
                     EGNITATHIGS"
     gene            complement(2650..2904)
                     /locus_tag="VI981_02780"
     CDS             complement(2650..2904)
                     /locus_tag="VI981_02780"
                     /inference="COORDINATES: protein motif:HMM:NF021452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HEY4479255.1"
                     /translation="MNEIKRKWDLLPKAKRKSCIDEIITFFKQKRDEQIGIIAAEDIL
                     DFFLQDIGTDIYNKGVDDSKELLKRQFENLEVDLDLLLNK"
     gene            complement(3061..3495)
                     /locus_tag="VI981_02785"
     CDS             complement(3061..3495)
                     /locus_tag="VI981_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479256.1"
                     /translation="MSERLPSSSYGELETRSGEHSMKSFDRDIDKLRSRIDRLSKATE
                     TLDIATQRDVLRFEANEIFLLLQKLFFYLEHDALRGNVGLAASEMRVIHEQLKNNQQE
                     QKLVLERMDKACTMLHTASECSRIADQIYELMVTVSDLEKKL"
     gene            complement(3706..4359)
                     /locus_tag="VI981_02790"
     CDS             complement(3706..4359)
                     /locus_tag="VI981_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479257.1"
                     /translation="MFSEKNFSLSPEKEQEPIIIIKKSTDAPKKVKENPFYDPEFWGR
                     ANSPDDIYLPDSDEAISFAIAAHEIGHLVDEGKRDDARLDNFEATRAEEQRAWDRGWE
                     YLQKYISEYYQDRPEIAPKIQQAFESIKDFLMQATYLSKAMYLEQGVLDNLSNEEIDK
                     VLKEKREKFFSENGEEFKQLFEEMKGEKIGVKPDWDKFVAVVKKAVEDIIKDNEKKE"
BASE COUNT         1129 a          967 c         1026 g         1406 t
ORIGIN      
        1 tttttttgct agacgtgctt aaatgatgag tcctgctagg cagctggata taaccctaaa
       61 cggaggtaag acgccatgca caagaactgg aaggtcatct gccagattcc ggacacccat
      121 gtgatgtggg cgctggcact gctcgacatg gattctgacc tcggtcggac gttcgtgggg
      181 cggtttccgg agtacttcac gcaggaggag caggcggggc cggtgccgct ctcagtgctc
      241 cgtcaggagg ccgcggagaa tgcggtgcat cagcatggag gcacagctgg cgacaaccac
      301 ctcgtgagct acaatgcgat cccggcatgg atgccccaat tgtacttcgc tgcgggcgcc
      361 gcgctcgagg agtacaagga gcaagcgatg ccggtgccgt ctgagctcga gaaggtgaag
      421 gtggtcggca cggtctgcgg gtggaagggc aagaagctcg tcctcgtcga ctacctgtac
      481 gatgggcaat ggtggttcgt ccctgccgaa ctcatcgccg tcgatcgctg atcgagccag
      541 ccccacttgt gtgctagtgc cccgtgtcgg aaagttcttc ggaacaatct gacgcggggc
      601 ctcttttttt caaaaaaatt tcaaaagggc tcaattcaaa ttcaaaatta tttgtttggc
      661 aaaattgccg ataacgttcc agcataacag aagttgcgaa gtgagcgtgg cggtataatt
      721 tttgcgaagc aaaccgccta agcgaacgag caatttgggt gaggggcgag ccgagccccg
      781 aacctgttat gctgtgttag ccgagggtga gccgtagcgt agcggaggca aaagcgcagc
      841 gaccccttgc gtaaataatt tgcccccatt tttcttgttt ttttataagt attctcacat
      901 ttattctggt ttttgataga cgaatgaatc atggatgacc ccagggaggt cgagtgattg
      961 atctcttagc ggtgctttat cgctcatttc tctccatata tgatcaacgg tggcatttat
     1021 tccaaatacg agacctcccg gcttcgttgc acgtactaat tcttgcgcaa gaacttcatg
     1081 gtatctttca ctgcctggga cagaagcaaa atctatacca ttgagcatga aatttgctga
     1141 gtcgtcccgc atgcgagcga caaaatctag catatctgct ttaacgctta gtttgtgtag
     1201 gtcgccgata tccccttcat tcaaagcggc gagcggatta actggataat cgccgtaatc
     1261 gcgatcgacg ttaatgtaag tcgtaacacc gaattgatgg tgtgccaagc tgaaagagcc
     1321 tgtgcggcca ccaagatcta caagtatatc gccagcgagc cgctgtttta acaggtcgcg
     1381 agcatgcggg aattcatcga agccatgcca ttgatcaaca aatcttgtac ctaaatacca
     1441 atctccttgg cgttgttctt gcgcaagaat tagttgtttg tattcttcgg gaacttggcg
     1501 agaagaatcc atgatgtctt ccagtgtgat tactcttctc tcttgttctg gtgtaaatcg
     1561 ttctttttcg ctcataaaaa taaatgtgag aatacctttt agaaaaatgg gggcaaatta
     1621 tttatgtcgg ctaacgtttc gggatatgcg atgttgcgat agcaatatgg gtgaagcgta
     1681 gcggaaccgc atatcccgtg ttaggcgctg tacccattaa caattacatc gaagaaaacg
     1741 ggcctccgca ttgaggacac ttgtctgagt taactagtcc gtaactggct ttgcaatttg
     1801 cgcaatatac atttgctgcg cttgtacgtc cgttgggatc tggaaccatt ggaactttgc
     1861 attgagagca aagtagctca ttactgccag cgacggcggc aatatcaact gtactattgc
     1921 attgtgggca tttgtaccca aaaaatgaac ctgtttgcat attttttctt gttaaaaatt
     1981 aggaacctat atgagttgca gtgatgttgc cttctccctc agctgtggat tttgtgccag
     2041 gcttgaaaaa tgtaggcctg gttgttttga tgccagttac attacctttg cctctagcag
     2101 tgtgttcgcc gtcgatggta gtaatgagag aatttaaact tttgttcaaa ttctgggtgg
     2161 cccattcttc atgctctttt ttccattgtt tcagctgatc tacagagaaa tcaataccat
     2221 tatttttatc gatcatatca gcgcaatttg aacaaagaaa aatgccatta tttgtagatt
     2281 ttctctgttc tgacgttaag tttgaatcat atcttgggcc accctctgct gcagccgtta
     2341 tgtgagctgc tttgcctatg tatataaatt tgtcctcatt ttcttctgat gggcaaagcg
     2401 ttaagctttt gcaatcagga tttgagcaga caaaggatgc ccgttttcct attacttcaa
     2461 ttgtatttct atcgaagtcg tctcgttttg acataatttt gtatgttaat gggtatggcg
     2521 cctaacgttc cagaatatgc gatgtttgcc agtggtataa tatgcccgaa gggcgaccgc
     2581 cggcaaatat gggtgtagcg accatcggga gcgaaaccgc atattctgtg ttatacgctg
     2641 tcacgaacac tatttattca gcaaaagatc taaatctact tccaaatttt caaactgtct
     2701 ttttagtagt tctttggaat cgtcaactcc cttgttgtaa atgtccgttc cgatatcttg
     2761 caaaaagaag tctagaatat cctccgctgc gattattccg atttgttcgt ctcgtttttg
     2821 tttgaagaaa gtgatgattt cgtcaatgca agactttctt tttgcttttg gaagtagatc
     2881 ccatttgcgt ttgatttcat tcatattagt gtgagtgatg gcatataacg ttcgcgtgta
     2941 tgagaagttg cgactgaagg gagcaatatg ggtggagcga agcggaaccg catacacgct
     3001 gttatatgat gtagtgaaaa gcaattttcc gtcagtaaat ttaaatactt acggcaactt
     3061 ttaaagcttt ttttctagat cgcttaccgt taccattaac tcatatattt gatccgctat
     3121 cctcgaacat tctgaggcgg tatgtaacat agtacacgct ttatccatcc tttctagtac
     3181 caacttttgc tcttgttgat tatttttaag ctgttcatga ataacgcgca tttctgaagc
     3241 tgctaggcca acattacccc tcagggcgtc gtgttctaga tagaaaaaaa gcttttgaag
     3301 aagtaaaaaa atttcgttag cttcaaaacg taatacatcg cgttgtgttg caatatctaa
     3361 tgtttctgtg gctttactta atctgtctat tcttgaccgc aatttatcta tatccctgtc
     3421 aaacgatttc atggaatgtt cgccagagcg ggtttctaat tcaccgtaag agctgcttgg
     3481 taatctttca ctcatagtaa tttttttaaa aaagttgctg taagtatttt aaatgcgtag
     3541 cattttgctt ttcactattt catctaacgt ccgcgtgtat gcgatatttg ccactggcat
     3601 aatatgccga aggcttacca gtggcaaata tgggcggagt cttgcggagg tacggagcaa
     3661 gtcgtagccg catacacgct gttatatgat gtttttgtaa aaatcctatt cctttttttc
     3721 gttatctttt attatatctt caacagcttt tttaacaaca gcgacaaatt tatcccaatc
     3781 aggctttact ccaatttttt cgcctttcat ttcctcaaaa agctgtttaa attcctctcc
     3841 gttttcagaa aaaaattttt ctcgtttttc ttttaatact ttgtctattt cctcgttgct
     3901 taaattatct aatacccctt gctcaagata catggctttg cttaaatacg tggcttgcat
     3961 caaaaagtcc ttaatgcttt caaaggcttg ctgaattttg ggggcgattt ctggtctatc
     4021 ttgataatac tcggatatat atttttgcag atattcccag cccctatccc aagctctttg
     4081 ttcttcggcg cgagttgcct caaaattatc taaacgcgca tcatctcttt tgccttcgtc
     4141 aacaaggtgg cctatttcat gagcggctat ggcgaaagaa atcgcctcat cggaatctgg
     4201 taaataaata tcatctggtg aattagcacg cccccaaaat tcaggatcgt aaaatgggtt
     4261 ttctttgact ttctttggag cgtcagtgga cttcttgatg atgattatgg gctcttgttc
     4321 cttttcagga ctaagagaaa aatttttttc tgaaaacata taaagggaat ttttacaaaa
     4381 atttcgtata acggggcgcg gctcatgcga tcgtcaccgc acacctccga acgctcgtga
     4441 ggaggggagg gatgtgcggg gatgtcgcat agccgctgtt agacgcagtc gcgagctgcg
     4501 cgagcgacaa ggcgaacgcg gctagtat
//
LOCUS       DATDSU010000084         7539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_589485, whole genome shotgun sequence.
ACCESSION   DATDSU010000084 DATDSU010000000
VERSION     DATDSU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589485"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VI981_02795"
     CDS             complement(<1..644)
                     /locus_tag="VI981_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY4479258.1"
                     /translation="MITNLAVEAQAIAPYVIEIRHELHSIPETRYETPKTRAVIRREL
                     GTFMPGVLIEESKGGIVANIGDIAGAPRLLFRADFDALPIAEKTGLAFISVNEGKSHA
                     CGHDCHTAMLLGLCNLIGRGWVTPKYNLRLVFQDAEENPGTNPEPISGGDRLVQDGVT
                     KGVERAYAIHVWNHPTGKPGVFLSRPGGLLGNSGRIKFAATSTTNVFPIRHVLAR"
     gene            complement(764..1015)
                     /locus_tag="VI981_02800"
     CDS             complement(764..1015)
                     /locus_tag="VI981_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479259.1"
                     /translation="MMSGTGEDFSFYLEEFRDKQIPGAFIMIGAANSEKGIPKGSHHT
                     EDFRVDEEVLSELASTHVAFVLKALEHYKKTAEQSSEKA"
     gene            complement(1034..2140)
                     /locus_tag="VI981_02805"
     CDS             complement(1034..2140)
                     /locus_tag="VI981_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY4479260.1"
                     /translation="MHNAEQIDLDAPSGAETGIIPTALERVRELQPEMEERYVDFHEH
                     PELGGQEVETARKIEQYLGQCGIEIKATGIGIEREAGEGKAWSKGTGIIGYIKGQDGG
                     PTIALRADMDALPINETDKNPHHSKTPGISHACGHDLHSASLMGAAKTLQELAEKKQL
                     PGNVVLLFQPSEEKTFQKESGAVQMVRALEENGLRDEIKAVLGLHVYRQLERGVYNLV
                     DKGVQISSSGEIDILLSAPGGHVKDAYVLPNLNLIQAEITSRLHKEFEHLGLAQKGVI
                     ASTNTEFEPRGYNVLAERSKANWVVRIASEDYKDISQEALGKIKKVVDEVVAEFGKDK
                     VNVELKPRPGYRPIVHREVDVTKLANDAVQAARP"
     gene            complement(2133..3551)
                     /locus_tag="VI981_02810"
     CDS             complement(2133..3551)
                     /locus_tag="VI981_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY4479261.1"
                     /translation="MNIEELKNKKWYIQSCNATPIMLMSAAESAREMQEKLGFGYTEF
                     LYEMEMDFGKMCYRVDDLERLGKIIYSKLDHDPKYFKKIRALHRRDQKISGKLFKEID
                     STDLGVYTDRELILLIKRAVQAVGTAVGVGHLIEPYALTIDAVLKEELSHFVRDAKDL
                     NQKFITLTTPTDISFVQEAEEALGRIKAAKGASREKYIRDYISGFEWSQNSYAGRAHI
                     DRAMIEKMLLEFKPGAKRDMRTLKREKKKIIKEYNLPKQLVARLETLEFLTSWQDERK
                     KYILIAVGYEDLLLAELARRMSIPIEHLRYLRVEELDSDIKSLAGELEKRRTGSIYVQ
                     RFEKPGSEVATGEEFLKIKKTLESSESLLVKELSGNAASVGKVIGRVKVCTTIASLAK
                     VEDGDVLVASMTRPEYMLAMKKAAAFVTDEGGITCHAAIVAREMHKPCVIGTKNATKV
                     LKDGDLVEVKGSHGLVIILEHA"
     gene            complement(3548..4942)
                     /locus_tag="VI981_02815"
     CDS             complement(3548..4942)
                     /locus_tag="VI981_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY4479262.1"
                     /translation="MSHETGRASWFCQHYFWANQFATADSNWPLPANLANICRNHIVK
                     TYFVVNSTEAFDRALERFLNDGSLPDKLEKYTTDITIETLKEIKDVEFGNLSNEEIAR
                     IVVFYFGQYNKIMRTAGTCRLLDRALIPRLRTLIPNDSNPDETVATASIPFKPTLTVN
                     EELAILTTAAKIDRKEISGNDIEVSIDEICRDFGFGILGYFDEKPRERSYYEERLKEI
                     LGKGARAELEAYNDKIKSEISHRESLKERVDEMGKKIISVASWASYIKDYFKSSTNKI
                     QYYGEAMFSEIAKRLKIDVSIKDLHPDEMVALLLGESVDLDKVRKRAEKSIVMAFDGV
                     VHVLESDDADLFERKYLTFENRGKKEFKGRVASKGHAKGMAKVVLGGADFHKLEQGNI
                     LVVANTSPDFVPIMRKAAAIVAEEGGLTSHVSVVSREFGIPCVVGISHITDFIKDGDV
                     VEVDAERGIVKILK"
     gene            complement(4996..5322)
                     /locus_tag="VI981_02820"
     CDS             complement(4996..5322)
                     /locus_tag="VI981_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479263.1"
                     /translation="MAELHSQTLASRWAKLPLPSQLANIGSEVSRLVLWIKKGNNEQV
                     ERSLARALELVDATLSDPRFGKKREEIEMLKHSIIGITKKNDLGIDPDSILAYFNPFF
                     SMAALK"
     gene            complement(5324..5671)
                     /locus_tag="VI981_02825"
     CDS             complement(5324..5671)
                     /locus_tag="VI981_02825"
                     /inference="COORDINATES: protein motif:HMM:NF039191.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HEY4479264.1"
                     /translation="MTIKIIEKKISKKELQEIAKEVFGDMVKCTADIVQMRLAVGGEL
                     HMDSNELLIKTGSSQQDVWGFNIYPDMPEADLLEFNSLVNIKPRLGNRSANIMDEKVQ
                     KKIREVVKKFVTD"
     gene            complement(5668..6081)
                     /locus_tag="VI981_02830"
     CDS             complement(5668..6081)
                     /locus_tag="VI981_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY4479265.1"
                     /translation="MSKQKVMVFGVFDLLHDGHIYFINEAAKLGELTIVVTPDQIVEH
                     TKGAVPAESIDMRVTNLKKQFPEHTIVHGDASEGTWSVLHTHKPDIIALGYDQRGLKS
                     ALKKHFAKSGKGYKLIEIGAHFPEKYKSSIKRQAK"
     gene            complement(6078..7451)
                     /locus_tag="VI981_02835"
     CDS             complement(6078..7451)
                     /locus_tag="VI981_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY4479266.1"
                     /translation="MDEKRIFKADKFSGLEGVNFALSVVLELIQRFNSLLPVKLHFYG
                     LTFDLVKNQSVEYIKNDLYEAETNKLLDYIKKNGTSLLRSIASETTKDAEVLRKLALE
                     EVKRIPKLSHFELVNRYTFFMNEYARRYAPPVITFLYETTLSERLEVSIRQRYENASE
                     IISSLLYNEYKSFMIESESALKKIYAVKNKAKQRSLIEHYKKQFFYIHSSYRAGPVLD
                     SDMVFEMAEEAAKYDINKNKRIKNTATLTDEEKLIASIFQFTEVIRDQRKKLNLIGSY
                     AMFRFLEEVSRRGTIPIEIAGRAFWNEYADLVFHPEESSKILEERTHASIVCDGASYH
                     YLPYNAISSRSEIENSDGVLRGSPASKGKVSGHARIVLGSEDFGKFQSGEILIATMTR
                     PDFVTIMKKARAVVTDEGGLTCHAAIVARELNIPCIVGTKLATKVFKDGDLIEVDSET
                     ATVRKIL"
BASE COUNT         1860 a         1855 c         1526 g         2298 t
ORIGIN      
        1 cgggccaaga cgtgccgtat gggaaatacg tttgtggtgc tcgtggccgc aaacttgatc
       61 cggccgctgt tgcccaagag tcctccggga cgggagagga aaactcccgg tttcccggtc
      121 gggtgattcc agacatggat ggcatacgcc ctttccacac ctttcgtcac tccatcttgg
      181 accagccggt cgccgccgga aataggttcc ggatttgttc cgggattctc ttcagcatcc
      241 tggaaaacga gccggagatt gtacttcggg gtgacccagc ccctgccaat aagattgcag
      301 agaccgagga gcatggctgt gtggcagtca tgtccgcagg catggctttt gccctcgttg
      361 accgagataa aggccaggcc agtcttctcg gcaatcggaa gcgcgtcgaa gtcagcccta
      421 aaaaggagtc gaggggcacc ggcgatgtcg ccgatattgg cgacgatccc tcctttgctt
      481 tcctcaatga gtacacccgg catgaacgtt ccgagttctc gccggataac ggcccgcgtc
      541 ttcggggttt cgtaccgagt ctccggaatg ctgtggagtt cgtgacgaat ttcgatgaca
      601 taaggtgcga tggcttgcgc ttcaactgcc aggttagtta tcaatagaac ctcactttct
      661 ttttggttgt tgtggtttag gaaatcgcgg gcttaagaag ttcaaactga atcgaacgta
      721 tcaaagagcc tacgcaaaac catagcacac aattaatgaa aaatcaagct ttttcggagc
      781 tttgttcggc cgtttttttg tagtgttcca atgcctttag tacaaaggca acgtgcgtac
      841 tggcaagctc ggagagcact tcttcatcta cgcgaaaatc ctccgtgtgg tgagagcctt
      901 ttggtatccc cttttcagag ttagcggctc cgatcataat aaatgccccg ggaatttgct
      961 tgtccctgaa ttcttcgaga taaaaagaaa aatcttctcc ggtaccagac atcatttctc
     1021 ggatgttttc tgatcatggg cgcgccgcct gcacggcatc gttggcaagc ttcgtgacat
     1081 caacctctct gtgtacgatc ggacgatatc ccggcctcgg ttttaattca acattcactt
     1141 tgtccttgcc aaattccgcg acaacttcgt caacgacttt ctttattttc cccagcgctt
     1201 cttgagaaat atctttgtaa tcttcagatg caattcgcac tacccagttt gcctttgagc
     1261 gctccgccaa gacattgtaa ccgcgtggct caaattccgt gtttgtagag gcgatgacac
     1321 ctttttgcgc aagacctaaa tgctcgaact ctttatgcaa gcgtgacgtt atttctgctt
     1381 gaattaaatt taagttaggc aggacatatg catctttaac gtgtcctccg ggagcagaga
     1441 gtagaatatc tatttctccg gaacttgata tttgaacacc tttatcgaca agattataga
     1501 cgcctctttc aagttgtcga tatacatgca gaccgagcac agcttttatt tcgtctctta
     1561 aaccgttctc ttcgagagct ctgaccatct gtacagcgcc gctttctttt tggaatgttt
     1621 tctcttcgct tggctgaaaa agtaagacga cattgccagg caactgcttt ttttccgcca
     1681 attcctgcag agttttagct gcacccatga gcgatgcgct atgtaggtca tgcccacacg
     1741 cgtgcgaaat tcccggagtt ttggagtgat gcggattttt atccgtctcg ttgatgggca
     1801 gtgcatccat gtctgcgcgg agcgcgatgg tagggccacc atcttgtccc tttatataac
     1861 cgatgatacc cgttcctttg ctccaagctt ttccctcccc tgcctctcgt tcaattccaa
     1921 tacccgtcgc ttttatttca atcccgcatt gaccgagata ctgctcaatt ttacgagcag
     1981 tttctacctc ttgcccacca agttcgggat gttcatgaaa atcaacataa cgctcctcca
     2041 tttccggctg gagttctcga acccgctcca gcgcagtcgg tatgatccct gtttcggccc
     2101 cgcttggagc atctaaatca atttgttctg cattatgcat gttctagaat tataaccaat
     2161 ccatgactgc ctttcacctc tacaagatca ccgtctttta aaacttttgt ggcatttttt
     2221 gtgccaatga cgcagggctt atgcatttcc ctcgccacaa tggcggcatg gcaggtgatg
     2281 ccaccttcgt ccgtgacgaa ggcggccgct tttttcatgg cgagcatata ttcggggcgt
     2341 gtcatggaag caacgagcac atctccgtct tccactttag cgagcgaggc gatggtcgta
     2401 cagaccttca cgcgcccgat gactttacca actgaagccg cattaccgga aagttctttc
     2461 acaagaagcg actccgacga ctcaagcgtt tttttaattt ttaaaaattc ctcacccgtt
     2521 gccacttcac tgcccggttt ttcaaagcgt tgaacataaa ttgatccggt tctgcgtttt
     2581 tcaagctcgc ctgcaagtga tttgatatca gagtcaagtt cctcaacccg aagatatcgt
     2641 aagtgctcaa tgggtatgga catccgtctc gcaagttcgg caagcaaaag atcctcatat
     2701 ccaacagcga tcaaaatata ttttttgcgc tcatcctgcc acgaagtgag aaactcgagt
     2761 gtctcgagac gagccaccag ttgttttggt aaattatatt cttttattat ttttttcttt
     2821 tctcgcttaa gcgttcgcat gtcccgcttt gcgccaggct tgaattcgag aagcattttt
     2881 tcgatcatag ctcgatcaat atgagcacgg ccagcgtatg agttttgaga ccactcaaat
     2941 ccgctgatat agtctcgaat atatttttct ctagacgcac ccttcgccgc tttgatgcga
     3001 ccaagtgcct cttctgcctc ctgcacaaaa gaaatgtcag tcggtgtagt cagggtaata
     3061 aatttttggt tcagatcttt agcatctcgg acaaagtgac taagttcctc ttttaatacc
     3121 gcatcgattg tgagtgcata cggttcaatt aaatggccga cgccgacagc cgtaccaacg
     3181 gcttgcacgg ctctcttaat aagcaagatc aattcacgat cggtgtacac tccgagatct
     3241 gttgaatcaa tttccttgaa caatttgccg gatatttttt ggtcacgccg atgaagcgct
     3301 cgaatttttt taaagtattt tgggtcgtga tcaagttttg aatatatgat tttgccgaga
     3361 cgctcaagat catcgacgcg atagcacatt tttccaaagt ccatttccat ttcatacaga
     3421 aattctgtgt agccaaatcc aagtttttcc tgcatttcac gggcactctc tgccgcggac
     3481 atgagcatga taggcgtcgc attacaactc tgtatatacc attttttatt tttcagctct
     3541 tcgatgttca tttaagtatc tttactatcc cccgctccgc atccacctcc acaacatctc
     3601 cgtcttttat gaaatcagtg atatgggaaa tgcctacaac gcaaggaatc ccgaattctc
     3661 ggctcaccac ggacacatgc gaggttaatc ctccttcttc ggccactatt gctgcagcct
     3721 ttcgcataat tggcacgaaa tcgggactcg tattcgcgac tacaagaatg ttgccctgtt
     3781 caagtttatg gaagtcggcg cctccgagaa caactttggc cataccctta gcgtgaccct
     3841 ttgaggctac gcgcccttta aattcctttt tcccacggtt ttcaaaagtc aggtactttc
     3901 gctcaaatag atcagcatca tcgctttcga gtacatgcac gacaccatca aaagccatga
     3961 ctatgctttt ttcggcacgt tttcgaactt tatctaaatc cacggattcc ccgagcagga
     4021 gcgccaccat ctcgtccgga tgaagatctt ttatcgatac atcaatcttc aacctttttg
     4081 caatctcaga aaacatcgcc tcgccgtaat attgaatttt attcgtcgac gacttgaaat
     4141 aatctttaat ataagacgcc cacgacgcaa ccgatattat ttttttaccc atttcatcta
     4201 ctctctcctt gagagattct cggtgtgaaa tttcggattt aattttatcg ttgtaagcct
     4261 caagctctgc tcgtgcccct ttgccaagaa tttccttcag ccgttcttca tagtagcttc
     4321 tttcccgagg cttttcgtca aaataaccaa gtatgccgaa accgaaatct ctacatattt
     4381 catctatcga cacttcaatg tcgtttcccg aaatttcttt ccgatcaatt ttcgccgctg
     4441 tggtcaaaat cgccagttct tcgttaacag tcagggtagg tttaaagggg atcgaggctg
     4501 ttgccactgt ttcgtcggga ttggagtcat ttgggattaa agttcggagt cttggaataa
     4561 gcgcgcgatc gagaagcctg catgttccag ctgtcctcat gattttgttg tactgtccaa
     4621 aatagaatac gactatccgc gcaatctcct cattactaag gttaccgaac tctacgtctt
     4681 taatctcttt tagtgtttcg atagttatgt cggtcgtata tttttcgagc ttgtcgggaa
     4741 gagacccgtc attcaaaaaa cgctcgaggg ctcggtcaaa tgcctccgta ctattcacga
     4801 caaaataagt cttaacaata tgatttcgac agatgttagc taaattcgcg ggaagcggcc
     4861 agtttgaatc cgcagtcgca aactggttgg cccaaaagta gtgctggcaa aaccacgatg
     4921 cgcggccagt ttcatggctc atttcatacc catttagatt ctgcgtaaat tttgttttgt
     4981 aaggaaaaag cggagtcatt tgagggcagc catggaaaaa aacggattaa aatacgcaag
     5041 aattgaatct gggtcaatcc cgagatcatt tttcttagtt attcctatta tcgaatgctt
     5101 tagcatttct atctcttctc tttttttccc aaaacgtgga tcagaaagtg ttgcgtcaac
     5161 gagttccagc gctcgcgcaa gagaacgttc aacttgctca ttgtttcctt tttttatcca
     5221 caaaaccagc cgtgagactt cagaaccgat attagcaagc tggctcggca gtggaagctt
     5281 cgcccaccga gaggcaagtg tttgcgaatg taattctgcc atgttaatct gtcacaaatt
     5341 tttttacgac ttctcgtatt tttttctgca ccttttcatc cataatattt gcagagcgat
     5401 ttccgagtct gggtttaata ttcacgagcg aattaaattc aagcaagtct gcttccggca
     5461 tatccggata aatattgaag ccccatacat cttgttggct cgatccagtc ttgattaaaa
     5521 gttcattgct gtccatatgt aactccccac caaccgccaa cctcatttgc actatatcag
     5581 cagtacactt taccatgtcg ccaaaaacct cttttgctat ttcttgtagc tcttttttcg
     5641 aaatcttttt ttcaataatt tttatcgtca ttttgcttgc ctcttgatgc tcgacttata
     5701 tttttctgga aaatgcgcac ctatttcaat aagtttatag cctttccctg atttagcaaa
     5761 atgttttttc agggcagatt ttaggccccg ttgatcgtac cctagagcga taatatctgg
     5821 tttatgcgta tgaagcacgc tccacgttcc ctcgctcgcg tctccatgaa caattgtgtg
     5881 ttcgggaaat tgctttttca aatttgtgac cctcatatcg atagattcag ctggcactgc
     5941 accctttgtg tgctcaacaa tctggtcagg tgtgactaca atagtcagtt caccaagttt
     6001 cgcagcttca ttaatgaaat agatatggcc gtcgtggagc agatcaaaaa ctccaaaaac
     6061 cattactttt tgtttactca taaaattttc cggactgtcg cggtttctga gtcaacttca
     6121 atcaaatctc cgtccttgaa aacctttgtc gccagtttag tcccgacgat acatggaata
     6181 tttaactctc gagctactat tgccgcatgg caggtaagtc ccccctcatc cgtgacaact
     6241 gctctggcct ttttcatgat ggttacaaaa tctggtctcg tcatcgtcgc gatcaatatc
     6301 tcacccgact ggaatttccc aaaatcttcc gaaccgagaa caattcgggc atggccgctc
     6361 accttaccct ttgacgccgg gctaccccga agcacaccgt cagaattttc gatttcactt
     6421 ctagaagaaa tagcattgta cggaagataa tggtaactcg caccatcaca gacaatagac
     6481 gcgtgagttc gctcctcaag tattttgctt gactcttcgg gatgaaagac gagatcggca
     6541 tattcgttcc agaaggcgcg tcctgcgatt tcaatcggta tggtgccgcg acgactcact
     6601 tcctctaaga agcgaaacat cgcataactt ccaataagat tcagtttttt tctctggtct
     6661 cggataactt ccgtaaactg aaaaatagat gcgattaatt tctcttcatc ggtcagggtg
     6721 gctgtatttt ttatgcgttt attcttgtta atgtcgtact tcgccgcctc ttcggccatt
     6781 tcaaacacca tgtcgctatc cagcaccggt cccgctcgat aactcgaatg gatgtaaaaa
     6841 aactgttttt tatagtgctc tatgagtgat cgttgtttgg ccttattttt aacagcataa
     6901 atttttttaa gtgcgctctc actttcaatc ataaaacttt tgtattcgtt gtagagaaga
     6961 gatgagataa tttcgctcgc attttcatat cgctgtctga ttgatacctc cagtcgttca
     7021 gagagtgtag tctcatataa aaatgtaatt acgggaggag cataccgtct tgcatactca
     7081 ttcataaaaa aagtataccg gttcacaagc tcaaagtgcg aaagtttcgg aattctctta
     7141 acttcctcga gtgcaagctt ccgaagaacc tcggcatctt ttgtcgtttc cgaagcaatg
     7201 gatctcaaca aacttgtgcc atttttcttt atgtaatcaa gaagtttatt tgtttccgct
     7261 tcatacaaat cattttttat atattcaacc gactggtttt tcacaagatc aaaagtgagc
     7321 ccataaaaat gtaatttaac cggcaaaaga gagttaaacc tctgaataag ttcgagaact
     7381 acagacagag caaaattgac gccctcgagc ccggaaaatt tgtctgcctt aaatatgcgt
     7441 ttttcatcca tgtggatatt tcaactatac agcccttacc gacgattagt aaagtttatt
     7501 atgtttatct tcgcaatcaa gactcccaac taagactta
//
LOCUS       DATDSU010000085         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1594123, whole genome shotgun sequence.
ACCESSION   DATDSU010000085 DATDSU010000000
VERSION     DATDSU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594123"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VI981_02840"
     CDS             <1..1444
                     /locus_tag="VI981_02840"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY4479267.1"
                     /translation="GIPYILDRIGNPPVYTREFGALLIKKRQSEFPHMPAIDFKIVDK
                     NDQSVPISKDLKIRFFGLTHSIPDSSGVIIETPHGDLLFTGDVRVENENGVPSEKELE
                     QYAHFKERNILLLAMDSTGIEKPGWSISEQRVIDTVDRLIKEAPSRVIIATFASQVER
                     IIAFIKIAKKYGRKVVIEGRSMKNNVEIIKFLKLADVEHIITIEQVADHPPHKLLMLA
                     TGAQGEEFAALMRISNKTHKHIGLTRSDTIILSSSVIPGNERAVSILKDNLYRHDCKI
                     ITYLDTDVHTSGHGKRGELEWIHKQIPYKFFMPVHGNHYMLKMHAELAQNLSVSADRI
                     MVPDNGSIVELSSNGSVIKMLKEKAPSGIVMVDGFAVGDIQEVVIRDRQMLAQDGMFV
                     IIATVNSSNGQLKKSPDIISRGFVYLRESQELLRQTRFIIKKTIEESARGANPINFDY
                     VKSNVTDSVAKFLFQKTAKRPIVIPVLLGV"
     gene            1703..2866
                     /locus_tag="VI981_02845"
     CDS             1703..2866
                     /locus_tag="VI981_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4479268.1"
                     /translation="MVGALRTHKVFIIYILGFLYSLHFTLPIYINSSFLSLFATEYYV
                     GVIYSISSILTILTFTFIGPFLNRYGNYKTAFIFFLIELICLIGLALNPSSLVTTVLF
                     VISFVSVAILTFSIDVFLESYSSYSSVGKTRGIFLTYANLAWLIAPLAVSFIMTDSDY
                     WKVYLAAAVLVVPILYIMSVTLRDFEDPEYEKIPFVKTFNQIKTDKNIYGIFIISFLL
                     QFFFAWMVIYTPIYLHEYIKFEWKEIGLMLSIVLLPFVITEIPLGKLADTRWGEREMM
                     SVGFLIIALSTALVSFVDSKSFLLWAAILLVTRVGASIVEVMSETYFFKKVDTSKVHL
                     LSLFRTMRPFAYLLAPLVAISLFNFMEIKYIFVILGLIMFYGLRFSLFLDDTK"
BASE COUNT          790 a          702 c          590 g          830 t
ORIGIN      
        1 cggtattcca tacatcttag atcgcatcgg caatcctccc gtatatacgc gagaattcgg
       61 cgccctgctt atcaaaaaaa ggcagtcgga atttccgcat atgccggcaa tcgatttcaa
      121 aatcgtcgac aaaaatgacc aaagtgttcc catttctaaa gatcttaaaa tcagattttt
      181 tggattaaca cattccattc ctgactcatc aggcgtcatc atcgaaacac cgcacggaga
      241 tctcctattc accggagacg ttcgtgtgga aaatgagaac ggagtgccga gtgagaaaga
      301 attggaacaa tacgctcact ttaaggagcg gaacatactt cttttggcta tggactccac
      361 aggcattgaa aaaccgggct ggtctatttc agaacaaaga gtcatcgaca cagtagatag
      421 actcatcaaa gaggctcctt ctcgcgtcat catcgctacc ttcgcttcac aagtagagcg
      481 cattattgcc ttcataaaaa tcgccaagaa atacggcaga aaagtcgtca tcgagggccg
      541 tagtatgaaa aacaacgtcg agattatcaa gtttctcaag ctcgctgacg ttgagcacat
      601 aattacaatt gagcaggttg cggatcaccc gccgcacaag ttgctcatgc tcgcgacagg
      661 agcccagggc gaagaatttg ctgcgctcat gcgcatctcg aacaagactc acaaacacat
      721 cgggctgacg cgttctgaca cgatcattct ctcgtcatcg gtgattccag gcaacgaacg
      781 ggccgtcagt attttgaaag acaacctgta tcgccatgat tgcaagatta tcacctactt
      841 ggatactgac gttcatacat ccggtcacgg caaacgcgga gagctcgaat ggattcacaa
      901 acaaatcccc tacaagtttt ttatgcctgt ccacggcaac cactatatgc tgaaaatgca
      961 cgcggaactt gcgcaaaact taagtgtttc ggcagatcgg atcatggttc ccgacaacgg
     1021 gtcaatcgtg gaattaagta gtaacggttc ggtcattaaa atgctcaaag aaaaagcgcc
     1081 gtctggaatt gtgatggttg acggttttgc ggtgggcgat attcaggaag tggtaatccg
     1141 ggaccgtcag atgctggcac aggacggtat gttcgttatt atcgcaacgg taaattcatc
     1201 aaatggccag cttaaaaagt cacctgacat catctcccgc ggtttcgtct acttgcgcga
     1261 atcacaggag cttttacgcc aaacacgatt catcatcaaa aaaacaatcg aggaatccgc
     1321 ccgcggcgcc aatccgatca acttcgatta cgtcaaatca aatgtaacag atagtgtcgc
     1381 caaattcctc ttccaaaaaa ccgccaaacg gccgattgtc ataccagtgc tactcggcgt
     1441 gtaattttgg attttggttt tggaaatcgc aaagtccagt ctcaatatcc acaccactcc
     1501 cctcatcaca caatagcaat cgcggaatgt ttcccctaaa agcaatttcg cgcgcatgtc
     1561 tttgtaatat acattaaaat tacgcggccg cgtaatttta atgtatattg acgggctgca
     1621 catttatcgc attatgttat taagggagac gtgtgtccat atccacggcg aatggccgcg
     1681 ttttcttgat atactagaga acatggtcgg cgctcttcga acccataaag ttttcatcat
     1741 ttatatactt gggtttttat actcgctcca cttcaccctg ccaatttata ttaactcttc
     1801 tttcctgtcg ctctttgcga cggaatacta cgtcggcgta atatattcta tttcatcgat
     1861 actcaccatt ttgacgttta cgttcatcgg cccttttctc aatcggtatg gaaattacaa
     1921 gacggctttt attttctttt tgatagagct tatctgtctc attggtctcg ctttaaaccc
     1981 aagctctctc gtgactactg ttctttttgt tataagcttc gtttctgtcg ccattctcac
     2041 ctttagtatc gacgtctttc ttgaatcata ttcaagctac agcagtgtcg gaaaaacaag
     2101 aggtatattt ttaacatacg ccaacctcgc atggcttatc gcgccgctcg ccgtcagttt
     2161 tatcatgacc gacagcgact attggaaagt atatctcgcg gcggccgtgc ttgtcgttcc
     2221 catcctctac atcatgagtg tcacacttcg agatttcgag gacccagagt atgaaaaaat
     2281 accattcgta aagactttca accaaatcaa aacggacaaa aatatttacg gcattttcat
     2341 tataagtttt ctattgcaat tttttttcgc gtggatggtc atctacactc ccatttatct
     2401 gcatgagtac atcaaatttg aatggaaaga gataggcctc atgctttcca tcgtcttatt
     2461 gcctttcgtc attacggaaa ttccacttgg caagttggca gacacacgct ggggggaacg
     2521 agaaatgatg tctgtgggtt ttttgatcat tgcactttcc actgctcttg tatcatttgt
     2581 cgactcgaaa agctttcttt tgtgggctgc aattttactc gtcacgagag tcggagcaag
     2641 catcgtcgaa gtgatgagcg aaacttattt tttcaaaaaa gtggacacaa gcaaagtaca
     2701 tctccttagt cttttcagaa cgatgcgccc ttttgcctat cttctagcac cgctcgttgc
     2761 catttccctc ttcaatttca tggaaataaa atatatattt gtcatactcg gccttattat
     2821 gttttacggt cttcgcttca gtcttttcct cgacgatacg aagtagaccc cgttagaagc
     2881 agatcgcgat caacgatcgc ggcttctaac gg
//
LOCUS       DATDSU010000086         3076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2245491, whole genome shotgun sequence.
ACCESSION   DATDSU010000086 DATDSU010000000
VERSION     DATDSU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245491"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..211
                     /locus_tag="VI981_02850"
     tRNA            140..211
                     /locus_tag="VI981_02850"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:173..175,aa:Glu,seq:ctc)
     gene            2404..>3076
                     /locus_tag="VI981_02855"
     CDS             2404..>3076
                     /locus_tag="VI981_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479269.1"
                     /translation="MEKKRTKRASGDALFVKRVFLTAKKIVSLLLVFLLLAPLWPTSI
                     AFAAGTPSILSVQGRLADASGNLLGGTGTTYYFQFSIWDNATVGSGSRLWPVASPATT
                     TTVVQSGVFNVNIGDTANGYPDVLDYDFSSNTNIYLQIEVSSDNNSSQTLTPRLRIAS
                     NAFAQLSAAVSGSTTPSSFGTTTPITNSIVTIEATSSIAIPLSIRANTSQSANLFQIQ
                     NGVGSA"
BASE COUNT          857 a          667 c          715 g          837 t
ORIGIN      
        1 taaagcagct tatggaacat cccgaggacg ccacgaaact tctcgtgcga caggaagacg
       61 aatggcagag gcaatacaaa taacgccccg ctccctagac agggcgcgct aaattcgtta
      121 ttctgtggaa gttttaattg gccctatcgt ctatcggcta ggacacagcc ttctcaaggc
      181 tggaagcagg gttcgattcc ctgtagggtc attcgcacaa aaatcagctc ctatttttct
      241 ggagcgattt ttgttgaatg agcctaagca gacaactgct tgtctgcgtc agggaatcga
      301 aaggctttgc gttatttatg aagcattcct atgctgaata aatcgcaaag gcgtactgaa
      361 gctgtaagct tcgattccct gtagggttat caacaacaat ctaaaaagcg aagaagacac
      421 aacacatttc acgctgtcct ttatgtcttt taaaggacag ggtaaaagac agagaaaaat
      481 gttaaatgtc ttttactggt gtgagccaga atgccccgtg cgtaagcacg gggatgaatg
      541 gccacagtta tatgggtgcg cggtggcgca cgcttcttcc agaaaaacat cctcggtgcc
      601 gcccgcgaaa tgccccgccc gtaagggcgg ggatagacgg gcggcatgtt ttactggaac
      661 agcagggaat ccacgggact ctgcccctgg gattggatgg tcggacaagt ttacaaaatt
      721 tcgtttaaaa cagatacata agaaggtctt atgtatctgg taactagcat cttgtgtaac
      781 gtgcgaaccg ttgtttttat cggggagggc ttataccagg aatagcacat aaatcttctc
      841 catggtcatt ttttttccta cgttttatga gaaattctat agctaaaaac taagcatgtt
      901 ccagcttaaa aacagagaaa aacggatgct atatttgctg agcaaaagga cgcggtttta
      961 gccgcgacct taaggcatgg agggagttcg atcagtagag ctttagcgac gatactcccc
     1021 acggcttctt tgtctttttg ttcgattact ttcttgagac atcagcaatt tttggtgcga
     1081 aggattatcg tcaggatggg ggatactttt tccgcaaaca atatatccgc cgagaaatgt
     1141 tttcggacgt aggagcttta aaacataggt ttatttactt cggatcagta gagtgtcgta
     1201 cccgcaagtc tctgcgccgc tcgccggtag atgtacataa tctacaacgt gaaagtaccg
     1261 gctctggcat caagcagtat aaggacttat gcataagaag gatgttgtga aagagataaa
     1321 acagtcgcca tgcatcagaa agtttctctg ggggtgttct caaaaatgtt ttacaactcc
     1381 cgccttaagg acgatgaaaa aatacacggt catgtcaggt atctccacaa aatataattt
     1441 ttgaaagaga gtgacctcta cgaaaaaaat ttgtacagtg gggtagtgcg gaagacatca
     1501 gtcgaacaaa aagtggagat caaaaaaaga ggtgacaaaa aaatttgata gaacaaatat
     1561 atttttagat acacagcatc gaaattattt aatgacacat atacggtggt cgatgaggga
     1621 agacttccga ctttttattt cgtgtttgag aaaacataaa aaattatttt tgtcggctca
     1681 tcgtgagtgc tcactatgac cagcatggtc tcgatcgcta cacaaaatat tttgttgtgc
     1741 acgtcgatca agtacatcgc gttaggtgtg agaaaatatc cgcatcaatt attttgtggg
     1801 acacatggtg tccacaaaac atatggcgtg gtgatgagac caaaaggtgc aagacaaaag
     1861 gactgacgga atattcttgg tatttttaca gcattcttgt ttgtgggtga gcgaaggatg
     1921 cccgttctca tcgttcgagc attttcgaag accccaggag tatcttgctt gaatacggga
     1981 ctttttatca tgcaacgttg tgttactcct cggagacact gttcacatag gcttgtgcag
     2041 acttactttg ttggcatttc ttcaacgaag attttttccc gcaccgttga ccctccgcta
     2101 gacagacaga ttcgttcaaa aattattgtg gtcgaccccg tatccctctt cagtaaaagt
     2161 ctttttaaaa ctaagttcgc agttttacct cccgaggttt tcagtgggtg tgtcgacaaa
     2221 taagcagagc ttattgcgag gaaattttga gggtgtgcgt cgtccatatt tcacccaaat
     2281 tccgagaaaa aaccgagatt caaaaaattt ctaaaatatg gctcaacagg acggccgact
     2341 tttccccagt tttctttaat actcgttgag aaataagggt ttttgcgaga taatggacag
     2401 tagatggaaa agaaaaggac aaagcgagct tctggcgacg ctttatttgt taagcgggtt
     2461 tttttgaccg caaaaaaaat agtttctctc cttctcgttt ttctactttt ggctccgctt
     2521 tggccaactt ccatcgcttt tgcagccggc acgccatcga tcttaagcgt tcaagggcgc
     2581 ctcgccgatg caagcggcaa tctccttggc ggaacaggaa cgacatacta ttttcaattt
     2641 tctatttggg acaacgcaac ggtcggaagc gggtcgcgtc tttggccggt tgcatcaccc
     2701 gcgacgacaa cgacagtggt gcagtcgggg gtgttcaatg tgaacattgg tgataccgcg
     2761 aatggttatc cggacgtgct cgactatgac ttctcaagca atacaaatat ttacttgcag
     2821 atagaagttt catcggacaa taattcttcg caaacactta ctccgcgttt gcggatcgcg
     2881 tcgaacgcct tcgcacaact ttccgccgca gtctccggtt cgacaacacc gtcatcattc
     2941 ggcacgacga cgccgatcac gaactcgatc gtcacgatcg aagcgacctc atccatcgcg
     3001 ataccgcttt cgatccgcgc gaacacatca cagtcggcaa atctttttca aattcaaaat
     3061 ggcgtcggtt ccgcac
//
LOCUS       DATDSU010000087         5443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1743564, whole genome shotgun sequence.
ACCESSION   DATDSU010000087 DATDSU010000000
VERSION     DATDSU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743564"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VI981_02860"
     CDS             complement(<1..869)
                     /locus_tag="VI981_02860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479270.1"
                     /translation="MQSKQKKKILFVITKSNWGGAQRYVFDIALALKFEFDVAVVLGG
                     DGPLKDKLVENGLRVISIEELSRDIKLKNEFKVLRNFIRLFKQERPDVVHLNSSKVGG
                     LGGFAARIARVPKIIFTAHGWVFNENRPWYQKMIILFLHWLMVLFTHKTIAVSQKTAD
                     DMRRLPFMSKKIICIYNGINKETYMRKNIAREALISIHPSRSIILKNKKNIWIGSVSE
                     LHKNKGIDVAIHAIGMFVKEWPNTLYLVISDGEEKKNLEALMAREKLMDHVILLGFIE
                     NPSRFLKAFDIFL"
     gene            1055..2422
                     /locus_tag="VI981_02865"
     CDS             1055..2422
                     /locus_tag="VI981_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEY4479271.1"
                     /translation="MASMTAVSNKDAFSLLIGDFAFLFLSLWVALLVRYGGAIPEGAS
                     YAVHLKSFIFVFVASLIVFYVAGLYEKKIMLRRGELPVIIFQSQLVNTALAMAFFYFL
                     PYVDIAPKTNLFIYLGVSLLFLVFWRMYLAPMIGGGGTVERAVIVGEGIEMDSITKEL
                     NNHRVYRTTVSGTVDVSRGDVREQSLIIREYVARQNVSVIIIDTTHEKCAPLFSELYD
                     LFLSHVRFIDLTTLYEDLFYRIPVTRISYGWCLANISVAPKVTYDVLKRIMDIVISLP
                     LAAGSLVLYPFIILLIKLDDRGSVFISQERIGKNDTLVRTSKFRSMTGNDAGEYGSDG
                     LTNLTVTRAGRFLRRTRLDELPQLWAVVAGKLSLVGPRPELPSVVKKYEKEIPYFAIR
                     HLIKPGLSGWAQIYGEHAHHGVDLDKTKDKLSYDLYYIKNRSFLLDLKIALKTIKLLL
                     SRKGI"
     gene            2427..3317
                     /locus_tag="VI981_02870"
     CDS             2427..3317
                     /locus_tag="VI981_02870"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY4479272.1"
                     /translation="MTKVVVTGGAGFIGSHIVDGCVEEGFDAHVIDISSPRKGHTNKK
                     ARYHEADIRNLKSITPIIKDALYVFHLAALPRVQYSIENPEETTETNVVGTMNVLAAA
                     QKGGVKRVIFSSSSSVYGDQPTLPFKEDMTPAPKSPYALHKLVGEQYCSVWNTVYGLE
                     TVSLRYFNIYGPRMSPQGSYPLAIALFIDQRKKGKPMTITGDGKQTRDFTHVSDVVKA
                     NILAAKSSTVGKGECINIGFGKGVSMLEITKLIGGPTKFIAPRLEPKHTLADNAKAKK
                     LLQWKPTISIEEGIKAIKNN"
     gene            3322..4149
                     /locus_tag="VI981_02875"
     CDS             3322..4149
                     /locus_tag="VI981_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479273.1"
                     /translation="MTKDKNKKTVVVIGYGYVGKAVYKFFADHYETLIYDTAMPELSA
                     TKEEINKRADLAVISVPTPMSKNKTADIHIVESTVAWLDVPLILIKSTVPPGTTEALA
                     KKYKKNIAFSPEYIGEGNYSVPFWKGYPDPTNMKNSDFVIVGGEKKNREKVLTYFKSI
                     LGAEPTYIQTTSTTAELAKYMENCFLATKVTFCNEFYDVAKSFGVDYNELREMWLLDG
                     RIGRSHTAVFEDKRGFGGKCLPKDINALVKATEKAGFKPKLLNKVIEVNDEIRSKHS"
     gene            complement(4210..4518)
                     /locus_tag="VI981_02880"
     CDS             complement(4210..4518)
                     /locus_tag="VI981_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479274.1"
                     /translation="MRQTIIVESDLIGKFAWELPPEMERNFKAIGEILRSQHPPEIAT
                     KMEEHGHNELRSFITSHLETLVGAYGIGGTTSNQILVALREYRNGLALAVQEILKNRP
                     "
     gene            4634..5071
                     /locus_tag="VI981_02885"
     CDS             4634..5071
                     /locus_tag="VI981_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479275.1"
                     /translation="MKIATFILIVCVAVGAFYAGARFYPINQGGNITPVPTKIESPPK
                     VTDDVVSNRLNFECTGERKFSLEYMQIDHSPGPGAVYIPETRTSVNLQLESQEIALLN
                     QPSFSDSFSSFISADKSTVLQIKNELASIVQNGKVMYEKCSVK"
     gene            5088..>5443
                     /locus_tag="VI981_02890"
     CDS             5088..>5443
                     /locus_tag="VI981_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY4479276.1"
                     /translation="MKSRITALIILLVAVAIGYFVYSSEMNDGRFKFKLGLDLAGGTL
                     LTYRADTSKIASEDISSSMQSLRDVIERRVNAFGVSEPLVQVEETGALGGNEHKLIVE
                     LPGVSDLQQAINLIGKT"
BASE COUNT         1558 a         1135 c         1189 g         1561 t
ORIGIN      
        1 aagaggaaaa tatcaaacgc tttcagaaat cttgacggat tttcaataaa accgagaagt
       61 atgacatgat ccataagttt ttctcgagcc atgagcgcct ctaagttttt tttctcttct
      121 ccgtcggaaa taacaaggta aagagtattc ggccattcct tgacgaacat tccgatcgcg
      181 tggatcgcaa cgtctattcc tttatttttg tggagctctg aaacagatcc gatccatata
      241 ttttttttgt ttttcaggat gatgctgcgt gatggatgta tggatataag cgcttcgcga
      301 gcgatgtttt tccgcatgta cgtttcttta ttgattccat tataaataca tatgattttt
      361 ttactcataa acggtagccg cctcatatca tctgcagttt tttgagaaac tgcaatcgtt
      421 ttatgtgtaa atagcaccat gagccaatga agaaacaaaa ttatcatttt ttgataccac
      481 ggcctattct cattaaatac ccaaccatga gcggtaaaaa taattttcgg cactcgtgcg
      541 atgcgtgccg caaagccgcc aagcccgccg acttttgaac tgttaaggtg cacgacatcc
      601 ggccgttctt gtttaaacag cctaatgaag tttctcaaga ccttgaattc atttttgagt
      661 tttatatcgc gtgaaagctc ctctatggaa ataacccgta agccattctc gacgagttta
      721 tctttgagag gaccgtcccc gccgagtacg accgcgacgt caaattcaaa cttcagagca
      781 agcgctatgt cgaaaacata acgctgggct ccgccccagt tggatttcgt aattacaaat
      841 aggatttttt tcttttgttt agactgcata tgttgagccc aaaagctctg aatgtccgat
      901 ttttattgag gaagcacaat acactagata acacctcgag tatacaatat gttgccgtac
      961 gagcgttttt cgtactgctg cgtaaaaaat gactcccgcg atacactaga gctcttatta
     1021 ttaaccgttg ccattttttt gtaacctata tattatggcg tcgatgactg ccgtcagcaa
     1081 taaagacgca ttttcgttgc ttatagggga ttttgcattc cttttccttt cgctttgggt
     1141 tgcactcttg gtgcggtatg ggggagccat accggaaggt gcatcatacg ctgtacacct
     1201 taaatcattt atttttgtct tcgtggcgtc tctcatagtt ttctatgttg ccggactgta
     1261 cgaaaaaaaa ataatgctac ggcgcggtga attgcccgtc atcattttcc agtctcaact
     1321 tgtcaatacg gctctcgcaa tggccttctt ttatttcttg ccgtatgtcg atattgctcc
     1381 gaagacaaat ctctttatat atctcggcgt ttcgctgttg tttctggttt tttggaggat
     1441 gtacctggct ccgatgatcg gcggaggcgg taccgttgaa cgtgctgtca ttgtcggaga
     1501 gggtattgaa atggattcaa taacgaaaga gctgaacaac caccgcgtgt accgtactac
     1561 agtgtctggc accgtcgatg tctcacgcgg tgacgtccga gaacagagtc taataattcg
     1621 cgaatacgta gcgagacaga atgtttccgt aataatcata gatacgacgc atgaaaaatg
     1681 tgcaccactg ttttccgagt tgtacgatct ttttctgtcg catgttcggt ttatcgatct
     1741 cacaacgctg tatgaggacc ttttctatcg cataccggtt acccggataa gttacggatg
     1801 gtgtcttgcg aacatttcag tcgcaccgaa agttacatac gatgtgttga agcgaattat
     1861 ggatattgtg atctcactgc cgctcgcggc gggttcgtta gttctctatc cgtttattat
     1921 tttattaatt aaattggatg accggggatc agtatttatt tcgcaagaac gtataggaaa
     1981 aaacgataca ttggtcagaa cttctaagtt tcgaagtatg acgggtaatg atgccggaga
     2041 atacggctcg gacggcctga ctaatttgac tgttactcgg gcaggccgat tcttaagaag
     2101 aactcggctt gatgaactgc cccaattgtg ggctgtggtg gcggggaaac tctcattagt
     2161 gggtccacgt ccagagttac caagtgtggt caaaaaatat gaaaaggaaa taccatattt
     2221 tgcaatccgt cacctcataa agcccggact ttccggttgg gcgcagattt acggagagca
     2281 cgctcaccat ggtgtggatc ttgataagac aaaagacaaa ctttcatatg atttatatta
     2341 cattaaaaat cgctcgtttc tgcttgatct taaaatcgca ttaaaaacta ttaaactctt
     2401 actgtctcgg aaggggatat aaaaaaatga ctaaagtggt tgtcacaggc ggggcaggat
     2461 ttattggatc acacatcgta gacggatgcg tcgaagaagg ctttgatgct catgttatcg
     2521 atatctcaag tccaagaaag gggcacacaa acaaaaaagc tcgctatcac gaggcggaca
     2581 ttagaaattt gaaatcaatt actcccataa taaaagacgc gctctatgtt ttccatcttg
     2641 ctgcgcttcc gcgcgtccag tattcgattg aaaatccaga agaaacgacc gaaactaatg
     2701 tcgttggcac aatgaacgtg ctcgccgccg cacaaaaggg tggagtgaag cgtgttattt
     2761 tttcttcatc aagttccgtc tatggcgacc agccgacatt gccttttaaa gaagacatga
     2821 cgcccgcgcc aaaaagccca tatgcgcttc ataaacttgt tggagagcaa tattgcagtg
     2881 tctggaacac ggtctacggt cttgagacgg tttcacttcg ctacttcaat atttatggtc
     2941 cgcgaatgtc gcctcaagga tcgtacccgc tcgccattgc tctttttata gatcagcgca
     3001 aaaaaggaaa acccatgact attacgggtg acggcaaaca aactcgagat ttcactcatg
     3061 tatccgatgt cgtaaaggcc aatatcctgg ccgctaaaag ttcgacagtc ggaaaaggtg
     3121 aatgtatcaa tatcgggttc ggcaagggtg tttcaatgct tgaaatcaca aaactaatcg
     3181 gaggcccgac gaaatttatt gcgcctcggc ttgaaccgaa gcatacgctt gctgacaatg
     3241 caaaagctaa aaaattgcta caatggaaac cgacgatttc gatagaagag ggaataaaag
     3301 ccataaaaaa taattaaaag tatgacaaaa gataaaaaca aaaagacagt cgtcgtcata
     3361 gggtacgggt acgtcggaaa ggcagtctat aaattttttg ccgatcacta tgaaacactt
     3421 atctatgaca cggcaatgcc tgagctatcg gctacaaagg aggaaataaa caagcgcgca
     3481 gaccttgccg tgatttctgt tcctacgcct atgagtaaaa ataagacggc agacatacat
     3541 attgttgagt caacagtggc atggcttgat gttccactta ttctcataaa gagcactgtt
     3601 cccccgggta cgacagaagc gctcgcgaaa aaatacaaaa aaaatattgc cttttctcca
     3661 gaatatatcg gagagggaaa ttacagcgtc ccattttgga agggctatcc tgatccgact
     3721 aacatgaaaa acagtgattt tgttattgtc ggcggtgaga aaaaaaatcg cgaaaaagtt
     3781 ctcacttatt tcaaatcaat tttgggcgca gaaccgacct atattcaaac tacgagtacg
     3841 acggccgaac tcgcgaagta tatggaaaat tgttttttgg ctacgaaggt tacgttttgt
     3901 aatgaatttt acgacgtcgc aaagtctttt ggagttgatt acaacgagct ccgcgaaatg
     3961 tggcttctcg acggtcgtat cggacgatct cacacggccg tatttgaaga taagagggga
     4021 tttgggggaa agtgtctccc aaaagatata aatgcactcg taaaagcaac agaaaaagca
     4081 ggattcaagc caaaactttt gaataaagtc atcgaggtaa acgacgaaat tcgatcaaaa
     4141 cattcctaat ctctcgttgg aatgatgtaa aaaaataaca aaaaaccaag ggctgccctt
     4201 ggctattctt tatggtctgt ttttcaggat ttcttgcaca gctagagcaa gtccattcct
     4261 gtactctcga agcgcgacaa gtatttggtt gcttgttgtg ccgccgattc cgtatgcgcc
     4321 gacaagcgtt tcgaggtgac tcgttatgaa ggaacgaagc tcattatggc cgtgttcctc
     4381 cattttggtc gctatttcag gtgggtgctg tgatcgaagg atttctccga ttgctttgaa
     4441 attgcgctcc atttccgggg gcaactccca agcgaatttt ccgatgaggt cgctctcgac
     4501 gatgatggtt tgtctcataa attcaggtgc tttcgttatc aaaaatacta tacaaaagta
     4561 tttttgtcaa ccccctagct tttatcatat ttggccgtta gaataacgaa atcttgttaa
     4621 atccatttaa caaatgaaaa ttgccacttt catccttatt gtttgcgtag ccgtaggggc
     4681 attttatgct ggggcgcgtt tttatccaat aaaccaaggc ggtaatatta caccagtgcc
     4741 aacaaagatt gaaagccctc ccaaagtcac ggacgatgtt gtttcgaaca gactaaattt
     4801 tgaatgtacc ggtgagcgga aattttcatt agagtatatg caaatcgatc attctcctgg
     4861 accaggagcc gtttacatac cggaaacaag aactagtgtt aaccttcagc ttgagagtca
     4921 agaaatcgcc ctcttaaatc aaccttcgtt ttcagattcg ttttcgtcat ttataagcgc
     4981 agataaatca acagttcttc agattaaaaa tgagcttgcc tcaatagttc aaaatggaaa
     5041 agttatgtat gagaagtgca gtgtaaaata atttacttca taatttcatg aaatcccgaa
     5101 tcacagccct cataattttg cttgttgccg tcgcaatcgg ttattttgtt tactcaagtg
     5161 aaatgaatga cgggcgattt aaatttaaac taggactcga tctggccggc ggcactttgc
     5221 ttacataccg agccgatacg tcaaaaatag ctagtgaaga tatttcgagt tccatgcaat
     5281 cacttcgcga tgttattgag cggcgtgtca atgcatttgg cgtttcagaa ccgcttgtgc
     5341 aagttgaaga aacgggcgcg ctcggtggaa acgagcataa attgattgtc gaattacccg
     5401 gggtaagcga tttacagcag gcgataaatt taatcggtaa gac
//
LOCUS       DATDSU010000088         3195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_84665, whole genome shotgun sequence.
ACCESSION   DATDSU010000088 DATDSU010000000
VERSION     DATDSU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84665"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VI981_02895"
     CDS             <1..265
                     /locus_tag="VI981_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479277.1"
                     /translation="FLITVGFRARYIAEGALAAGMHESKILQFEDAEEAGEALELMLE
                     QGDVVLVKGSQSMRMEKTVEAVMAHPEDKEKLLVRQEKEWVGR"
     gene            complement(296..556)
                     /locus_tag="VI981_02900"
     CDS             complement(296..556)
                     /locus_tag="VI981_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HEY4479278.1"
                     /translation="MEIVYKPTFVRQFDKLELTLQEETLEKITLLKNTKNHKLLKVHK
                     LHGPLAGRYSFSVNYKIRIVFSYTGRKEVVLLAIGDHAVYDA"
     gene            complement(563..772)
                     /locus_tag="VI981_02905"
     CDS             complement(563..772)
                     /locus_tag="VI981_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY4479279.1"
                     /translation="MTTLSVPLPAHLEEMVKKMAKQRGSNKAAIVREALERFAEEEAI
                     SAVLKAEKEQPLRGDLRVLARKLRS"
     gene            complement(1161..1589)
                     /locus_tag="VI981_02910"
     CDS             complement(1161..1589)
                     /locus_tag="VI981_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479280.1"
                     /translation="MSDIIISILIPAAVSFVVSILIFEHKVRFKELHIERAKVIRELW
                     SRINDTYEKLIEDKQEDNLKKIEGKRHLPIDSWTEIREMKTYFNKNSIYFNDEIAKEF
                     NKVFYKFQGNSTRVDITDLRNNLEALKKPLEKEFRKLLGV"
     gene            complement(1605..2936)
                     /locus_tag="VI981_02915"
     CDS             complement(1605..2936)
                     /locus_tag="VI981_02915"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEY4479281.1"
                     /translation="MRKPLTALSILFISLAFVVPAPALAQTDTGPTPGSFWYGIITTF
                     ENINLFLTFNSEKKAEKALGYAERRLTQAKAAAEGENSKAVETALADYETKINLATES
                     SKKIKDNERAEKLFTSIADNTSKHQEVLSEVLEKIPEEARGAIAKAIEASKRGQEEAM
                     KQVTELKGEVEQLKNDIAELKQNDSDEQTAEIEKLKREVEELKKKPAATPPVVTPTPT
                     TPQATPDKAFLNQTPPPIETWAELEARYFVSADQKGWTSQTITNALGEKRYYRKEGNQ
                     WVRKNSEAEATQPYEDFTRPAVAPSPTQLRRLMAMCSLSDELAATCNTQTFISGYFTN
                     ITFRTLVDELLIRAEQKLAEQKVQNELAQQRYLLYLQTINQPAIAPTTRYETYSLPVY
                     SGGLKLESPQLNTPIKWNVQWTGGGAGFIQDSSGNTTNFQCDSLLGRCFSL"
BASE COUNT          762 a          700 c          722 g         1011 t
ORIGIN      
        1 cttcctcatc accgtcggct tccgtgcccg ttatatcgcg gaaggcgcgc ttgccgccgg
       61 catgcatgaa agcaagatat tgcagtttga agacgccgaa gaggcgggag aggcacttga
      121 gcttatgcta gagcaggggg atgtcgtgct ggtcaaaggg tcgcagtcga tgcgcatgga
      181 gaaaacagtc gaggcggtca tggcgcatcc ggaagacaaa gaaaaattgc tcgtacggca
      241 ggagaaggag tgggtgggga ggtagaaaag agcctattat tttttgagca aagacctatg
      301 cgtcgtatac ggcgtggtcg ccgatcgcca gcaacacgac ttcctttctg cccgtatacg
      361 aaaagacaat tctgatttta tagtttacag aaaaactata gcggccagct agtggcccgt
      421 gtagtttatg aaccttgagg agtttgtgat ttttcgtatt ctttaaaaga gtaatttttt
      481 ctaaggtttc ctcttgaagc gtcagttcaa gcttatcaaa ctgacgaaca aaagtcggct
      541 tgtacactat ttccatacga gcttatgagc gaagctttcg agcaagcact cgaagatcgc
      601 ctcgaagagg ctgctctttc tctgctttaa gcacggcaga aatcgcttct tcctccgcaa
      661 aacgctcaag cgcctcgcgg actatggcgg ctttatttga accgcgctgt tttgccattt
      721 tttttaccat ctcctccaaa tgagcaggaa gcggtacgga tagtgtggtc ataaatatat
      781 gatgttataa tattacaata tcatatacgt atgggattta tctgtcaatt acgtaggtaa
      841 agctcggcaa tggagccctt cctggcgagg actgttcttc ctaggaggga agacaggaag
      901 aagctattag ggcgggagtg ggtggggagg tgattggggg ttgttactga gctagctact
      961 gagcacgctc agtagctcag aacacgatgc ttctgagtac tttgcacacg tggtgcctcg
     1021 ccatgcaaaa ttctttaagg aatggcgaga tgcaaatctg tatgtatttg ccccacccgc
     1081 cgagtgcgca cacatcatcc ccgccgtttt acaatttttt cctaaaacgg cggggattag
     1141 ctcccgtaat ttatcctttc ctatacaccc aatagtttgc gaaactcttt ctcaagtggt
     1201 ttcttcaacg cctctaagtt gtttcttaaa tcggtaatat caacccgcgt tgagtttcct
     1261 tgaaatttat aaaagacttt attgaactcc tttgcgatct catcgttaaa atatatcgaa
     1321 tttttgttga agtatgtttt catctctcga atttctgtcc aggaatcaat tgggagatgt
     1381 ctcttgcctt caatcttctt caggttatcc tcttgtttat cttcaatgag tttttcatac
     1441 gtgtcattta ttctgctcca taattctctg attactttag cccgctcaat atgaagttct
     1501 ttaaatctaa ctttgtgttc aaaaataaga attgaaacaa caaatgatac tgcagcggga
     1561 attagaatag aaataattat atcgctcata tttttgattt caatctagag agaaaaacat
     1621 ctgccaagga gactgtcaca ttggaaatta gttgtgttac cagaagaatc ttgtataaac
     1681 cctgcgccac cgcctgtcca ttgaacattc cattttattg gagtgttgag ttgtgggctt
     1741 tctaatttta gacctccgga atagacaggc aacgagtatg tttcgtatcg tgttgtaggc
     1801 gctattgctg gctggtttat cgtttgtaaa tataaaagat aacgttgttg agcaagttca
     1861 ttttgtactt tttgctcggc tagtttttgt tccgctctta ttagcaactc gtcaacaaga
     1921 gttcgaaacg taatgttggt aaagtatcca ctgataaatg tttgagtgtt gcaagtagcc
     1981 gcaagttcat cactcaaact acacatagcc atcaagcgcc ttaattgagt cggggatgga
     2041 gctacggctg gacgcgtaaa atcttcataa ggttgtgtcg cctcggcttc actatttttt
     2101 cgaacccatt ggttgccttc ttttcgatag tatcttttct ctcccaatgc attagtaatt
     2161 gtttggcttg tccaaccctt ttgatctgcc gatacaaaat atctagcttc aagctctgcc
     2221 catgtttcaa ttggaggagg ggtttggttt aagaatgctt tgtcaggcgt tgcttgtggt
     2281 gtagttggtg ttggagtgac aacgggcgga gtagctgctg gtttcttttt caactcttcg
     2341 acttctcttt ttaacttctc gatttcagcc gtttgctcgt cgctatcatt ttgttttaat
     2401 tccgcaatgt catttttcag ttgctcaact tcacctttaa gctcggttac ttgcttcatg
     2461 gcttcctctt ggccgcgctt actcgcttca atcgctttcg cgattgctcc ccgcgcttcc
     2521 tctgggattt tttccaaaac ctcggatagc acctcttgat gttttgaggt gttatccgca
     2581 attgatgtga ataatttctc ggcgcgctcg ttgtctttta tcttttttga cgattctgtc
     2641 gcgagattta tttttgtttc gtaatcggca agcgcggttt caaccgcttt cgaattttcg
     2701 ccttctgcgg cggctttcgc ctgtgttagc cgcctctctg cgtagcccaa cgccttctcc
     2761 gcctttttct cggagttaaa tgtcaaaaag agatttatgt tttcaaaagt ggtgatgatt
     2821 ccataccaaa aactgcctgg tgttggtccg gtgtctgtct gtgctaacgc cggagcggga
     2881 acgacaaaag cgagcgagat aaacagtatt gataacgcgg ttagcggttt tctcatgtag
     2941 ttacatcata gcacttagta ttttttctac taacgccctc agtggaacgc ttcgcgtact
     3001 aattttatat ggtcacaagt tactaagtag tttggcgaat tcacaccaaa ctacgtaagt
     3061 acttgccgac cccgcctcgc gatcgcacgt gctcgtgcaa tattgctgcg gcttcatcgc
     3121 atagcaaaaa acctgccgca gggcaggtct tttgcttgcg atgaccccac cgggaatcga
     3181 acccggattt agagc
//
LOCUS       DATDSU010000089         6608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1264461, whole genome shotgun sequence.
ACCESSION   DATDSU010000089 DATDSU010000000
VERSION     DATDSU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264461"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VI981_02920"
     CDS             complement(<1..966)
                     /locus_tag="VI981_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY4479282.1"
                     /translation="MQRTLIKDAAQNIGKEVLLKGWVDIRRDHGKLIFLDLRDMSGKV
                     QMVALPNHTEAHEAASKVRSEWVIEVEGKVNERPAKNAKPGLNGNLEIEILELKVLNE
                     AETPPFDLSGDGYEIGEETRLKYRYLDLRRPRLAKNIRMRDKIISFFRDYMHKNDFVE
                     IETPILMKGTPEGSREYIVPSRLEKGKFYALPQSPQQFKQLSMVAGFERYFQIARCMR
                     DEDTRGDRQPEFTQLDYEMSFVTQEDVLQYTEAMFIELAKTLFPEKQISTVPFPKITY
                     KESMEKYGNDKPDLRKDKNDKNELAFCWVLDFPMFEKTDDGKIQAV"
     gene            1071..1718
                     /locus_tag="VI981_02925"
     CDS             1071..1718
                     /locus_tag="VI981_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY4479283.1"
                     /translation="MGIRKHAFAEGEFYHIYNRGVEKKPIFTQQKDVDRFFKSILEFN
                     NITPIGSIYENSFVKESSIYRPLVRFVAYCLNPNHYHFILEPIVNKGIEKFMQRLGTG
                     YTKYFNRRYKRSGVLFQGKFKSSHIDSNEYLLHVSAYVNLNNKVHQLGSQASKLVRSS
                     WNEYVDPARQGICDKRIILDQFSTANQYVEFANSSLPQIIERKNSLKEIQSLLID"
     gene            2118..2609
                     /locus_tag="VI981_02930"
     CDS             2118..2609
                     /locus_tag="VI981_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479284.1"
                     /translation="MNKNPTLVGSYRVRVPAAMTSSVLISAYGGLISNDCLMLLAKEW
                     DDCEREMLRIKVYSKFEDAFNVHKFAQGFTPSDRPRFRQTTIAELVALGAKERDLGLA
                     TFVYGLGSAYINPEERLACGMVEPTFYYPGLESMDGSRQVLARAIHNPWDCDTHIALV
                     ELS"
     gene            complement(2800..4662)
                     /locus_tag="VI981_02935"
     CDS             complement(2800..4662)
                     /locus_tag="VI981_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4479285.1"
                     /translation="MTENQESKEEKKEEEVAEEAKPAQEQEKTKVGKKELKESFSVLL
                     QYLRKYRKETIALSIFGIISAVGNGIVPYVAGRFFDSIITPEVVTFFGALMPLYVALL
                     IVWAIVQLVTYLVDWQINIKSENFSNAVWVDYWGRGIAHLLMLPMAFHKAKKIGEVGE
                     KINRAAFSLETIIGRIVIDLAPQFLSIVIALVIGFYIKPILAVALLAGILIYSIVLFR
                     SVAPIAEIQREYQTHLWRAFGDSFDLIGNAHAIKQATAEEYEANRMSTLLKGPVLNLW
                     GKMNKIWSNLTLIQRVIILATQLFIFVASVYFISRGAMTLGELLAFNAYAAMMFGPFV
                     VLGRNWQTIQNGVIQLEEAEAVLREVPENYHPSGAPVSFNLKGTIKFDNVYFHYEEKK
                     PVLENISFNVTAGEIVALVGESGVGKSTLVELISGYQFPIQGQVRIDGEPIEKIDLIS
                     LRQQIGIVPQEVVLFNDTILHNIRYGNFSATDEAVKNAARSAHAYDFIEKFPEKWQQI
                     VGERGVKLSVGQKQRVAIARAILRNPKILILDEPTSALDAGSEKIITESLEKLMRGKT
                     TFIIAHRLSTVRKADKILVFKDGRLIEQGKHDELLKIPGGEYRRLYELQIGLHG"
     gene            complement(4724..5068)
                     /locus_tag="VI981_02940"
     CDS             complement(4724..5068)
                     /locus_tag="VI981_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEY4479286.1"
                     /translation="MEINHTPTEEEVLLKTALEKRGLRVLSQVPDGHKFIDLAIPSAR
                     INIEVDGIQHLTDSYQIVRDLKREHYSDDLGYDTIHIQNLFIRNNLEQIADALYGAAH
                     YRAQKIKYHSQI"
     gene            complement(5079..5618)
                     /locus_tag="VI981_02945"
     CDS             complement(5079..5618)
                     /locus_tag="VI981_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479287.1"
                     /translation="MNNFKIPTDNLYKFIAIAGILLVLSSALFYLWFVVNTRMEDSML
                     ELEQKSLSIDADMSDKILSVLAQNTGQITNSSEALNYALKLIDESRNTFASLKKKNSE
                     ITTNDESTKYMTRVVKLTCSAMLVVGIILACCGFILWYRRLQRYLDMIIKKKALTESA
                     EKTKATKKEIKQKIGVTVT"
     gene            complement(5622..6575)
                     /gene="trpS"
                     /locus_tag="VI981_02950"
     CDS             complement(5622..6575)
                     /gene="trpS"
                     /locus_tag="VI981_02950"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY4479288.1"
                     /translation="MPNKKKILLSGVKPTGRPHIGNYFGAMKQFVDLQHDYECYFFIA
                     DLHALTLVQNREEMSQAIYDIALDYLAIGLDPTKVTIFKQSDVPAHSELAWIFECITS
                     MPYLMRAHAFKDAEAKNKEISVGTFNYPMLMAADILLYDTDVVPVGQDQKQHLEITRD
                     TAEKFNRTFGQTFRLPEPLILKEVAIVPGIDGQKMSKSYKNTIPLFSTDEEIDKLVMS
                     IVTDSSGETPKNVYEIHKLFRSETELKKLYEEKKGKYKDLKQALADDIKTFIKPLRSR
                     RDSLAQAKSAVLKILQSGAEKANSIASKKLSEAKKRVGVIF"
BASE COUNT         1784 a         1480 c         1380 g         1964 t
ORIGIN      
        1 caccgcctgt atttttccat catcggtttt ttcaaacatc ggaaaatcga gcacccagca
       61 aaatgcgagc tcgtttttat cattcttgtc ttttcgcagg tcgggtttat cgttgccgta
      121 cttctccatg gattccttgt acgtaatttt tgggaatgga accgtagaga tttgcttttc
      181 aggaaaaagt gttttcgcaa gctcaataaa catcgcttcc gtatattgta aaacatcttc
      241 ttgcgtcaca aaagacattt cataatcgag ttgcgtaaat tctggttgcc gatcaccgcg
      301 tgtgtcttcg tcgcgcatac atcgagcaat ttgaaaatat cgctcaaaac cggcaaccat
      361 cgaaagctgt ttaaactgtt gcggcgactg cggtagagcg taaaattttc ctttctccag
      421 tcgcgacggc acgatgtact cgcgagagcc ttcgggcgta cctttcataa gaattggcgt
      481 ttcaatttct acaaaatcat ttttatgcat gtaatcacga aaaaatgaaa tgatcttgtc
      541 tcgcatgcgg atattttttg ccagtcgcgg acgacgcaga tcgagatagc ggtatttgag
      601 gcgagtttcc tcacctatct catagccatc tccagaaaga tcaaaaggtg gcgtttctgc
      661 ttcgtttaga actttgagct cgagtatttc aatctcaaga tttccgttca ggcccggttt
      721 cgcgtttttt gccggccgct catttacctt tccttcgact tctatcaccc attcactgcg
      781 aactttacta gcagcctcat gtgcttccgt gtgattaggc aatgccacca tttgcacttt
      841 tccggacatg tctcgcaaat caagaaaaat cagtttgccg tggtcacgtc gaatatccac
      901 ccaacctttg agcaggactt ctttgcctat attttgagcg gcatctttga ttaacgttct
      961 ttgcatgagc attattctag cagttttttt cgcatatcca agaaggaaac ccacacttgg
     1021 aagctcactt ggaagccagg cttccaagtg gtaaaatgtt tgaaattgac atgggtatta
     1081 gaaaacatgc atttgcagag ggagagtttt atcacatcta caatcgaggt gtggagaaaa
     1141 aaccaatctt cactcagcaa aaagacgttg accgtttttt taaaagcata ctcgagttta
     1201 acaacataac tccaattggc agtatttatg agaactcatt tgtaaaagag tcctccatat
     1261 atagaccgtt agttcgattt gtcgcatact gcctcaaccc aaatcactac cattttatcc
     1321 ttgaaccaat tgtgaacaag ggtatcgaaa aattcatgca gcgtctggga actgggtaca
     1381 ctaaatattt taaccgacgc tataaaagat cgggagtatt gtttcaaggt aaattcaaat
     1441 catcacacat tgattcaaac gagtatcttc ttcacgttag cgcatacgta aatctgaaca
     1501 acaaagtaca ccaacttgga agccaggctt ccaagttggt gcgatctagt tggaatgaat
     1561 atgtcgatcc tgcgcgacaa ggtatctgcg ataaaagaat aatccttgat caattcagca
     1621 ctgccaatca atatgtggag ttcgccaatt cctccctgcc acaaattatt gagcgaaaaa
     1681 atagtctgaa agaaatccag agcttgctaa ttgactgatt gaattaacta aagcggcacc
     1741 atcactgccc cactgcccca cttggaagcc aggcttccaa gtgggttttc agaatcgtga
     1801 tctcgttaaa aaaggaaggg ccggtcacgt gaggtgcgcg accggctcga tactgctgcc
     1861 tcaagaacac gactctaacc tgcgcatgca tgttcgtttt tttcgcaggt gatacgtaaa
     1921 gtgacggtac tggccgacac gatgtcagtt cccgtttgtt acgtatccgt cgttgtgagg
     1981 ttttcctcta gtccggaaag gattgtagca cgcttggaga ctaagtctcc gagtcgggtt
     2041 cgatctccaa gctaactgtg cgcagtattg ccaaacctgc tgaagtatgt agtatttgga
     2101 aagacacatt acaacctatg aataaaaacc caacgctcgt cggttcctac cgcgttcgcg
     2161 tacctgccgc catgacgtcc tctgtgctca tatccgcata cggcggatta atctcaaatg
     2221 actgtctcat gcttctcgca aaagaatggg atgactgtga aagggaaatg cttagaatca
     2281 aggtgtacag caagtttgag gatgcattca acgtacacaa atttgcacaa ggattcactc
     2341 cgtctgatcg cccacgattt cgacagacaa caatcgctga attggtcgcg cttggtgcaa
     2401 aggagcgcga ccttgggctc gccaccttcg tgtacggtct cggaagtgca tatatcaacc
     2461 cggaagaaag actcgcgtgc ggcatggtgg agccaacatt ttattatccc ggcctcgagt
     2521 caatggacgg atcgcgccaa gtactcgccc gggcgattca caatccgtgg gactgcgaca
     2581 ctcatatcgc tcttgtggaa ctgtcttgac gagcgaacac acgaaaccgc aaaaaatagt
     2641 gcggtttttt tacggcagtt atctttactt ggagacttag tctccaagaa atacagtttc
     2701 acaagagaga atctatccgc acacctacaa ctataatctg ctatagcaga ttatagttgt
     2761 aggtgtacac catccatgat aagtatccaa gactggaagc tagccatgca ggccgatttg
     2821 gagctcgtag agtctgcggt attcgccacc cggaatttta agaagttcgt cgtgcttgcc
     2881 ttgctcgatc aggcggccgt ctttgaaaac aagaattttg tcagccttgc gaaccgttga
     2941 gagacggtga gcaatgataa atgttgtttt acctcgcatc agtttttcga gcgactcggt
     3001 gataattttt tccgatccgg catcaagtgc tgaagtcggc tcgtcgagaa tcaggatttt
     3061 tggattgcgt agtatcgccc gagcaatggc cactcgctgt ttctggccaa cggaaagttt
     3121 tacaccgcgc tcgcccacaa tctgctgcca tttttcagga aacttttcta taaaatcata
     3181 tgcatgagcg gagcgagctg cgttcttcac cgcttcgtcg gtggcgctga aatttccata
     3241 tcgaatattg tgaagtatgg tgtcgttaaa aagcacgact tcctggggca ctattcctat
     3301 ctgctgtcgc aaagaaataa gatcgatttt ttcgatcggt tcaccgtcaa tgcgaacctg
     3361 accttgaatt ggaaactggt agccggaaat aagttctacc aaagtagact tgccgacgcc
     3421 cgactcccct acaagcgcca cgatttctcc agccgtaaca ttgaaactga tattctcaag
     3481 cacaggtttc ttttcttcat agtgaaaata tacgttatca aatttaatcg tgcccttcag
     3541 attaaacgac accggagcgc cactcggatg ataattttca ggcacttcgc gaagcacggc
     3601 ttcagcctcc tcgagctgga tcactccgtt ttgaatcgtt tgccagttac gaccgagcac
     3661 aacaaatgga ccgaacatca tggcggcata agcgttgaaa gccagaagtt caccgagtgt
     3721 cattgctcca cgggaaataa agtaaacaga cgcaacaaaa ataaaaagtt gcgtggccaa
     3781 aataattacc cgctgaatga gcgtcaaatt gctccaaatt ttattcatct ttccccacag
     3841 attgaggacg gggcctttga gaagcgtgct catgcggttg gcctcatatt cctctgcagt
     3901 tgcttgcttg atggcatgag cgttaccgat gagatcaaag ctgtctccaa aagctcgcca
     3961 cagatgtgtc tggtattcac gctggatttc tgcaataggc gcaacagatc gaaaaagaac
     4021 gattgagtaa attaaaatac cggcaagcag cgccaccgca agaatcggtt ttatgtaaaa
     4081 tccgataaca agagcaatca cgatcgagag aaactgcgga gcaagatcaa tgactattcg
     4141 accaataatg gtttcgaggg aaaaagcggc acgatttatt ttttctccaa cttcaccgat
     4201 tttcttcgcc ttgtgaaagg ccataggcaa catgagtaga tgagcaattc ccctgcccca
     4261 ataatctacc cacaccgcat tcgagaaatt ttcactttta atatttatct gccagtccac
     4321 gagatacgtg acaagctgaa cgattgccca gacgatcaaa agcgccacat aaagcggcat
     4381 aagcgcgcca aagaaagtga caacttcggg cgtaatgatc gagtcaaaaa atcgtccggc
     4441 aacataaggc acaatgccat ttccaacggc tgaaataatg ccaaaaatgg agagagcaat
     4501 tgtctctttt ctatatttac gtagatattg caaaagcaca ctgaagcttt ctttcagctc
     4561 ctttttacct acttttgttt tttcttgttc ctgcgctggt ttggcttctt cagctacctc
     4621 ctcctccttc ttttcttcct tagattcttg gttttctgtc atttcgcgag tatatcaaat
     4681 atagatactt ttggatatgt tatgtctagt taaaagtcgt gaactaaatc tgtgagtggt
     4741 attttatttt ttgcgcccga taatgagcag ccccatatag tgcatcggcg atctgctcga
     4801 gattattgcg aataaacaaa ttttgaatat ggattgtgtc atagcccaag tcgtccgagt
     4861 aatgttccct cttcaaatct ctaactattt gatacgaatc agtgagatgt tgaataccat
     4921 caacttcgat atttattctt gcactaggga tggctaaatc aataaattta tgcccgtcgg
     4981 ggacttgaga taataccctt aaacctcttt tttcaagggc agtttttaaa agtacctcct
     5041 cctctgttgg agtatgattt atttccataa ttaattattt aagtgacagt cactccaatt
     5101 ttttgcttta tttccttttt tgtagctttt gttttttctg ccgattcggt taatgctttc
     5161 ttttttatta tcatgtctaa atatctttga agtcttcgat accacaatat aaaaccacag
     5221 caagcaagaa taattccaac tacaagcatg gcgctgcacg ttaactttac tactctcgtc
     5281 atgtactttg tcgattcatc atttgttgta atttcgctat tctttttttt aagactagcg
     5341 aacgtgtttc tggactcatc aattaatttt aacgcatagt tgagtgcctc tgatgaattc
     5401 gtaatttgac ctgtgttttg tgctaagacg ctgagaattt tatctgacat atctgcgtca
     5461 atactcagag atttttgttc taattcaagc atggagtctt ccatacgagt atttaccaca
     5521 aaccatagat agaacaacgc ggacgacaac accagcaata tccccgctat cgcgataaat
     5581 ttatacaaat tatctgtagg tattttgaag ttgttcatag tttaaaaaat tacgccgact
     5641 cttttctttg cttcagagag ttttttagac gcaatggaat ttgctttctc agcaccagat
     5701 tggagaattt tgagaacagc ggatttggct tgcgcgagtg agtctctacg actgcgcaga
     5761 ggttttatga aagtttttat gtcgtcggcg agcgcttgtt ttagatcttt gtacttccct
     5821 tttttctctt cgtagagttt cttcaattct gtttcagaac gaaagagctt gtgaatttcg
     5881 tacacatttt tcggcgtctc gcccgatgag tcggtaacga tagacattac aagcttgtct
     5941 atttcttcgt ccgttgagaa aagcggaatg gtgtttttgt agctcttcga cattttttga
     6001 ccgtcaattc cgggcacaat agccacttct tttaaaataa gcggttcggg cagccgaaaa
     6061 gtttggccga atgttcggtt gaacttttcg gctgtgtcgc gagtgatttc caaatgctgt
     6121 ttctgatctt gtcctactgg cacgacatct gtgtcgtata gcaaaatatc agcagccatg
     6181 agcattggat agttaaatgt ccccacggaa atttctttgt ttttggcttc tgcatctttg
     6241 aacgcatgcg cacgcatgag gtatggcatg cttgtgatgc actcaaatat ccaagcgagc
     6301 tcgctgtgag ccggaacatc agattgtttg aaaatagtta ctttcgtagg atcgagcccg
     6361 attgcaagat aatcgagcgc aatatcataa atggcctgtg acatttcttc cctattttga
     6421 accaatgtaa gagcgtgtaa gtcggcaata aaaaagtaac attcgtaatc atgctgaaga
     6481 tccacgaact gtttcatggc tccaaaataa ttgccaatat gcggtcttcc ggtgggtttt
     6541 acaccgctta atagtatttt tttcttgttt ggcatttgca aatactagca aattaaggct
     6601 atgtcgga
//
LOCUS       DATDSU010000090         7172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1428932, whole genome shotgun sequence.
ACCESSION   DATDSU010000090 DATDSU010000000
VERSION     DATDSU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428932"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1722
                     /locus_tag="VI981_02955"
     CDS             295..1722
                     /locus_tag="VI981_02955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4479289.1"
                     /translation="MKVGAKGGGRCIGGSHYEYCSHGEDVALGVDYGAIQREKKTTYP
                     AMIQRETPAFTETHDHLDHCGGVPAFLREHPETVPYMTLMALRGTMLQMRDTLKISRS
                     RAEVDTLRGRKPTPAPFSEEDVQNFFKRARQKTVLNSDWFSPMPGYRMSFRSAGHKPG
                     AAMLLIVFPDGTRWIHACDFSIEESELVLGAKVPDDFKNPDGMTIECTYGSRDEDRQP
                     PDRKQELVRLYQEVTRVFDRSGKLLVPHFASTLQNIAFQLARWGFRTYIDGMGRDFSY
                     LYELTSPWCENDKPFRFDDLDLKDTLSPVGKMGNDREERQSLVERPGPFSIVTSSGMM
                     VGGPSVFYAERFLEDSRNAIILPGYQAPGTMGRMLAELERGRSITFEHEPMAYTKYGK
                     RLLREGWSETHQILCDVLPFRLSGHSGRKMVAEWVCQINPKKVVEVHGDPEAHEGMKW
                     QIQQLNPAIEVFSLGNDEEFDFGAP"
     gene            1918..1988
                     /locus_tag="VI981_02960"
     tRNA            1918..1988
                     /locus_tag="VI981_02960"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1950..1952,aa:Gly,seq:tcc)
     gene            2316..2720
                     /locus_tag="VI981_02965"
     CDS             2316..2720
                     /locus_tag="VI981_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479290.1"
                     /translation="MVKVIFGGLLPLIVMAANFVGYAVCSTLFRGMIEPPTLMLMMLV
                     EMIVFFILPKKFLQKPFAAMGFYIPPLILLSPLLFAMLSASDGNAKYAMAGIVICIFA
                     YTTFLAIRAAILSLKGKDNKMINLVGREVAYR"
     gene            3018..3383
                     /locus_tag="VI981_02970"
     CDS             3018..3383
                     /locus_tag="VI981_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479291.1"
                     /translation="MKLFACLKCLKTSGLTSSLTFFVGTKNVKRCGLCGKKFQLVPKD
                     SAWLFKTIKDMASMAMRNACNATVPHAPHRGYHEYGIMFNPAGRVGCNCTHKAHEFLE
                     SVLILFSEPRKLKKILRRR"
     gene            3518..3745
                     /locus_tag="VI981_02975"
     CDS             3518..3745
                     /locus_tag="VI981_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY4479292.1"
                     /translation="MAKREFTAVYQKRGNRYIAWIEEVPGVNTQGKTRKETKENLKEA
                     LFLILESNRKLASKQRGGLMFREPLCIGVPA"
     gene            4256..4501
                     /locus_tag="VI981_02980"
     CDS             4256..4501
                     /locus_tag="VI981_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479293.1"
                     /translation="MKNSIIGLKELRENADTYISQIERGKTFIVVRRSKPVFKIAPPD
                     ESDELWEPVIDFTEFYKEGIPASQLLKKLRSLNGKSR"
     gene            4485..4727
                     /locus_tag="VI981_02985"
     CDS             4485..4727
                     /locus_tag="VI981_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479294.1"
                     /translation="MGNLDKQLRKFSPKERAEIEDLVEKILERDLTGLNCKKLKGSRN
                     LFRVRKGRIRIIFELIGNKEPNIMTIERRNEDTYKL"
     gene            4997..5350
                     /locus_tag="VI981_02990"
     CDS             4997..5350
                     /locus_tag="VI981_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479295.1"
                     /translation="MEEFPTRKSLKPIIEKMVGGGPGPNEKILEEISTRVTKGMDEAD
                     AMRLELYLMDIMRVVGEGGDKKPKELLATVKNGDSYANLNTVMDVPDSTTLEFIAEVR
                     EKCLLKNKSPLSIEK"
     gene            5459..5815
                     /locus_tag="VI981_02995"
     CDS             5459..5815
                     /locus_tag="VI981_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479296.1"
                     /translation="MLEPYFETGTEGTVWSIFEDGKEGYDGLHMIEEGDHLTIYGENN
                     EVLFDDDIRCDRQAGWTEYPLNPGNGQPSALGLWIHWTQAGWQPDDWAKLFFHPYLQG
                     NEDKTALRAELTKKER"
     gene            complement(5924..6124)
                     /locus_tag="VI981_03000"
     CDS             complement(5924..6124)
                     /locus_tag="VI981_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479297.1"
                     /translation="MFDFIITADSAAEAGELAADNRKTLYDFYGERELVFCKRIGIAQ
                     GLSRPTVILVGRPSEYVSPRFP"
     gene            complement(6296..6472)
                     /locus_tag="VI981_03005"
     CDS             complement(6296..6472)
                     /locus_tag="VI981_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479298.1"
                     /translation="MTYDKQNLDELIEKLLKFGEDAEELGYWQSIFDDLEPGEQEALI
                     SNLRDELEKLEKLK"
     gene            complement(6469..7011)
                     /locus_tag="VI981_03010"
     CDS             complement(6469..7011)
                     /locus_tag="VI981_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479299.1"
                     /translation="MNRFEKLQTPDTSRERETIMENAVEDRGNLAARIFKSRVADLAG
                     NFIPGIDIPKMAFEASIGKTSSSERLSKRQRTAYAIISGGTALAYTLQLLGMTAEAVT
                     LRTAVAGLAQVEFGPEFLREAGEKARGKFPRVAPILEKTANYAAEKRELVALCIKNAQ
                     EKLRRMGNLEGIPLDEKLSL"
BASE COUNT         1994 a         1530 c         1717 g         1931 t
ORIGIN      
        1 gaggcccggt agggtcaaca actagccccc gctaagtggg gcaagcaaca ggcccggagg
       61 ggaggagtga agtcttactt cctccctccg ggccattttc atagatgttt aatgaatatc
      121 tggatgcggg ccattgaacg gctatgaaag tggaagagta aagcaagcgg cttttaaaaa
      181 tctctcaaag agtttacata tggctgtata ggtttattgc cgtatgtaag ctcttcacaa
      241 cttaatagag caattcacca aacattacag gccaaaaagg cccgggagag agacatgaaa
      301 gtcggtgcta agggtggagg gagatgcatt ggaggttccc attacgagta ttgttcgcac
      361 ggtgaagatg tggcgctcgg cgtcgactat ggcgcaatcc agcgcgagaa gaagacgacc
      421 tatccggcga tgattcagcg ggagacgccc gcattcaccg agacgcatga tcatcttgat
      481 cactgcggag gagttcccgc gtttctgcgc gagcatcccg agacggttcc gtacatgacg
      541 ctgatggctc ttcgcggaac gatgttacag atgagagata cgctgaagat ttcgaggagc
      601 cgcgcggaag tcgataccct gcgcggacgc aagccgacgc ctgcaccgtt ttcggaggaa
      661 gatgttcaga atttcttcaa acgtgctcgg cagaagacag ttctgaattc ggactggttc
      721 tcgccgatgc cgggttaccg gatgagcttt cgctccgcag gtcacaagcc gggtgcggcc
      781 atgcttctca tcgtgtttcc agacgggacg cggtggattc atgcctgcga tttttcgatc
      841 gaggagtcag agttggttct gggcgcaaaa gttcccgatg acttcaagaa tccggacggc
      901 atgaccatcg agtgcacgta cggctctcgc gacgaggacc gccagcctcc tgaccggaag
      961 caggaactcg tgcgtctcta tcaggaggtc acgcgggtct tcgaccggag cgggaagctt
     1021 cttgttcccc acttcgcgtc aactcttcag aacatcgctt ttcagctcgc gcgctggggg
     1081 ttcaggacat acatcgacgg catggggcgc gacttctcct atctgtatga actgacctcg
     1141 ccttggtgcg agaacgacaa gccgtttcgg ttcgacgacc tcgacctgaa ggatacgctt
     1201 tctcctgtcg gcaaaatggg taacgaccgg gaggagcgtc aaagccttgt cgagaggccg
     1261 ggaccttttt ccatcgttac gtcatcaggc atgatggtgg gggggccatc ggtgttttac
     1321 gccgagcggt ttctcgagga ttcaagaaac gccatcattc tgccggggta tcaggcccca
     1381 ggcacaatgg gacggatgct tgccgagctc gagcgcggca gaagcattac gttcgagcac
     1441 gagccgatgg catacacgaa atacggcaaa cgcctgctcc gcgaggggtg gtcggaaacg
     1501 caccagattt tgtgcgatgt cctgccgttc aggctttcag gacactccgg ccgcaagatg
     1561 gttgccgagt gggtatgcca aatcaacccg aagaaggttg tggaggtaca cggcgacccc
     1621 gaggctcacg agggaatgaa gtggcagatt cagcagttga atcccgccat tgaagtgttc
     1681 tcgcttggaa acgacgagga gttcgatttc ggcgctccct aggggagata gtagcaagtc
     1741 cggcgttgcc gggcgggctc gagtgcgtca tgcgctcgag cctcttttaa tttaatgcta
     1801 aattatgaat ggcaaatgaa gagtgtgcca gcgacttgaa attaggcatt taatatatat
     1861 aatggggata taaatttcaa agctagagcg tatttagaat ttagaatttt aaatactgcg
     1921 ggtgttgtat aatggtaata cgcgaccctt ccaaggtcga gctacgggtc cgattcccgt
     1981 cacccgctca ttaggggaaa ctcacttctt tttacgagta ctgaaatgga ttttcgatag
     2041 tgccaatgca gatttttatc tcgtaaggac aagttcgaag ctgctctttg gagcagtttt
     2101 tgtttttgtt cattatttcc cgaaaagaat aaaaaattgg cttcacgagc gagttttatc
     2161 aaattcgaag atttgttata ccattccggt tcatctgcct caagcagcag tgttttaatt
     2221 tacgggtgag gagaaattgg aatcagttga ctactataag tcttgatagt atctttgaat
     2281 aagaggcact tttacaaatt tgcgaggcaa ggctgatggt aaaagtaatt tttggtggac
     2341 ttctgccgct tattgtgatg gctgctaatt ttgtaggata cgccgtatgc tcaactctct
     2401 ttcgaggtat gattgagcca ccaacgctga tgttaatgat gcttgtagag atgattgttt
     2461 tcttcatcct gcctaaaaag tttctgcaaa aaccctttgc agcgatgggt ttttacatcc
     2521 caccccttat actgttaagt ccgttgcttt ttgccatgct gtcagcaagc gatggcaacg
     2581 caaaatatgc aatggcagga attgtaattt gtatattcgc ttacaccacg tttcttgcga
     2641 tacgagcagc catactttcc ctgaaaggga aagataataa gatgattaac cttgttggtc
     2701 gcgaagtagc atatcgttag aaaaagtatc cgcttcctct tgaagcggtt ttatattgca
     2761 agacttggta tcaaaagctg tacacctttc tcaaaacaga ttccctattc gaccgaacct
     2821 gtcggctcaa tcaaggggct ttgtaacgcc cctcttttgc tccgccttcg ttaaaacttt
     2881 tgcggatagg taacgcttgg tggacaagta acgcctgctt gccggtaggc aggctgtgca
     2941 cgagccgcgt gctttacaaa acagcatgaa agtgctactt ttgttttagt tcatcacaaa
     3001 accgataggg acattaaatg aaattgtttg cttgtttgaa atgcttgaaa acatccggct
     3061 taaccagttc attgacgttc tttgttggca caaaaaatgt aaaaaggtgc ggtctttgcg
     3121 ggaaaaaatt ccagcttgtt ccaaaagata gcgcatggct tttcaaaacg attaaagata
     3181 tggccagcat ggcgatgaga aatgcttgta acgccacagt tcctcatgcg ccgcacaggg
     3241 gctatcacga atatggaatt atgtttaacc ccgcaggacg ggtcggatgc aactgcaccc
     3301 acaaggccca tgagtttttg gagagtgttc ttatactttt ttcagaaccg cgaaaactaa
     3361 aaaagattct ccgccgccga taaaggcgga tttattttac tcaaaacaag ttccctattc
     3421 tgtcgagcct gccggctcaa tcaatgggct ttaggctagc gtcctgcatt aaccagctcc
     3481 cttgataaaa attttatagt gggtataatt ggagtatatg gcaaaacgag aatttacagc
     3541 agtatatcaa aaacgcggaa atcgctatat tgcgtggatt gaagaagtcc ctggtgttaa
     3601 tacgcaagga aaaacaagaa aggaaactaa agaaaacctt aaagaggcgc tgtttttgat
     3661 acttgaatct aatagaaaac ttgcatcaaa acagcgtggt ggattgatgt ttcgggaacc
     3721 actttgcata ggagtacccg cctaagattt tatgaatcgt cgcgcattac ttttgggcgt
     3781 gggctttggt caatcttggt atagtcgcca ttgtagcttt tgcactatat tttactcata
     3841 gcctgtggtc gtttcttggt cttgtgttct tattttctac aagaagtaag acggtaaata
     3901 caaaatgtcc gaagtgcgac cataagtttg ttgcggtaga aaaagatgac gatgaggacg
     3961 agaaagaaaa gtaggaataa gttaaaccgt tggttcgccc agcggttttt ttattaccaa
     4021 actataagtg agtaacgggg tggagcaaat tcaagcattt ccttatttac ttgcataaaa
     4081 caggttctgg ttcaataaat ttaccattaa acattttatc ctgaacgaag ttgaaggata
     4141 acgtccctcc tccgcgcaag gctatggacg ggcaatgcgt gcatttatcc tgagcgaagc
     4201 cgaaggacta acccgctctt gacacaaggt ttatcctaag ataaacttaa gatatatgaa
     4261 aaactcaatt ataggtttaa aagaactaag agaaaatgcc gatacataca tttcccaaat
     4321 agagcggggg aaaactttta ttgtggtcag gcgatcaaag ccggtattca aaattgctcc
     4381 acccgatgaa tcagacgagt tatgggaacc agtgatagat tttactgaat tttacaaaga
     4441 aggcattccc gcgagtcagc tattaaagaa attgcgttcg ctcaatggga aatctcgata
     4501 aacagttaag aaaattttcg ccgaaagaac gtgctgaaat cgaggatctt gttgaaaaaa
     4561 ttcttgagcg agatttaacg ggtctcaatt gcaaaaaact gaaagggtcc agaaatcttt
     4621 ttcgagtccg aaaaggaaga atccgcatta tttttgaatt gataggaaac aaggagccga
     4681 atataatgac cattgaacgc agaaacgaag acacgtacaa attataaatt ctaccgtgca
     4741 tggtattata tcgaattcaa catcactccg tattgaaaat cagatttttc cgcttgaaaa
     4801 aaggttctta ttcggctttc gctcataatc tttatagtaa tcccgagtct attcaaggat
     4861 ggttcgacaa actcaccatc aattatttat cctgaacttg ttgaaggata acttggtcta
     4921 cccccacact taaatttaag tgtgggggtc tactaacctg cttgtaataa gattattaag
     4981 atgctatatt catattatgg aagaatttcc cacaagaaaa tcattaaaac ccataattga
     5041 aaaaatggtg ggtggcgggc caggaccaaa tgaaaaaata ttggaagaaa tttcaactag
     5101 ggtgacaaaa ggaatggatg aggcagacgc gatgaggctt gaattatatt tgatggacat
     5161 aatgagggtt gtgggtgagg ggggagataa aaagcctaaa gagcttcttg caacggtgaa
     5221 aaacggagat tcgtatgcaa atctaaatac ggtgatggac gtacccgact caacaactct
     5281 tgaatttatt gcagaggtaa gagaaaaatg ccttctcaaa aataaaagtc cgctatctat
     5341 agaaaaatag gttcttaggc cgtgccccag cttgtgaatt tgcaaaatag cacaatcgtg
     5401 ctatttttat tttagttcaa aaaccaacac aaagaggtgt aaagtgaaaa tcattggggt
     5461 gctggaaccg tattttgaaa ccggaaccga aggcaccgta tggtcaatat ttgaagacgg
     5521 aaaagaaggg tatgatggac tgcatatgat tgaagaagga gaccatctca ccatatatgg
     5581 agaaaataat gaagttcttt ttgatgatga catacgctgt gatcgacaag ccggatggac
     5641 ggagtatccg ctcaatcccg ggaatggcca gccatccgct ctcggtcttt ggattcattg
     5701 gactcaagca ggctggcaac ccgatgattg ggcaaaactt ttcttccatc cgtatcttca
     5761 gggaaacgaa gacaaaacag cattgcgagc cgaactgaca aaaaaagaac gatagcaaca
     5821 ccgcgcggta tctccggcgg tttttatttt gtaaaaatat tcttaataag tatacataaa
     5881 aaagccgccg aataatgtca gcggctatac tgtgaaaata atattacgga aatcgcggtg
     5941 agacatattc agaaggcctt ccgacaagaa tgacggtagg acggctaagg ccttgcgcta
     6001 ttccgatacg cttgcaaaaa actaattccc gttcgccgta aaaatcatat agtgttttcc
     6061 tgttgtcggc ggcaagttct ccggcttctg ccgcggaatc tgccgtgatg ataaagtcaa
     6121 acacctctct atcttcccct tttttcttcg gaacagcccg gatccgcaga tgaaaaagcc
     6181 cccttcatgt tttctccaaa gaacgcgccc gaacgtgaaa atatcttact cccctttttc
     6241 cttttttgca agaatctttt tgaaactaat aaaaagcgcg aagagtttcg tcgcgctatt
     6301 ttaatttctc aagtttttcc aattcatccc ggaggttaga aatgagcgct tcttgctcac
     6361 caggctcaag gtcgtcaaaa atgctctgcc agtagccaag ctcttctgca tcttctccga
     6421 acttgaggag tttttctata agctcgtcaa gattttgctt gtcgtatgtc ataaacttaa
     6481 cttttcatcg agcggaatgc cttctaggtt tcccattcgt ctgagttttt cctgcgcgtt
     6541 tttaatacac agagcaacta attcgcgctt ttcagccgcg tagttcgccg tcttttcaag
     6601 aatgggagct actcgaggaa attttcctcg ggccttttcg cctgcctcgc gcaaaaactc
     6661 aggaccgaac tctacttgag caagaccggc aacagcggtt cgcagggtaa ctgcttctgc
     6721 cgtcatgccg agaagctgaa gtgtataggc aagcgctgtc ccgccggata tgattgcata
     6781 cgctgtgcgc tgtctttttg ataaccgctc actacttgac gtttttccga tagaggcctc
     6841 aaacgccatt tttggaatat caattccggg aataaagttt cccgcaagat cagccacacg
     6901 cgatttaaaa atgcgagccg caaggtttcc gcgatcctcc acggcattct ccattattgt
     6961 ttcacgttct cgcgaggtgt cgggtgtttg taatttttcg aaacgattca taatgaaatt
     7021 attatacgac aaaaaacgct tgatttcaag aaaaaatttt gaaaaatctg aacagctgcc
     7081 ggtttggcgc gccctgtctt ctttgtggtt ttttcatgag gttgggctag ggtgggtgag
     7141 ctttgttata ttccatcttc ctttgcaata tt
//
LOCUS       DATDSU010000091         3973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1266201, whole genome shotgun sequence.
ACCESSION   DATDSU010000091 DATDSU010000000
VERSION     DATDSU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266201"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VI981_03015"
     CDS             <1..1268
                     /locus_tag="VI981_03015"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY4479300.1"
                     /translation="LKKHSYKNTETVHLWQAFEKVSKKKVEQMMRTWTSRPGYPVLEA
                     KLLNNKLFLSQSRFFASPASKQKAKDKTIWQIPVSIEDKKGPWAKINIGETGFFRTAY
                     SKELLEKLYAPVKGKILEASDRLGIVRDLFALSEAGTIPTTDALDFLSAYKSEDNYMV
                     WVEIATGLSKIEQLVTKTPMEEDFKKFMHDLFSPLALRFGWNKKKKELHSATLLRPLV
                     LSRAGLAGNKKIIKEAKEKFALLQRGGSVSPDVRSSVYTILARHGGTKEYKILIKKYK
                     KETLHEEKNRIGNALGFFPNPKILAFVCKFSLSKDVRMQDTIGIISSVGTNPAGRDVW
                     WNFVKKNWKLLVSRYGDGGHTLARLVKAISGSPEAKHLFSFKKFFKTRDAPGAKRSIE
                     QVLERLEGNILWLKRDKKLIHTFLSTLLD"
     gene            1464..1859
                     /locus_tag="VI981_03020"
     CDS             1464..1859
                     /locus_tag="VI981_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEY4479301.1"
                     /translation="MSKGMNKNTTIKTVRLIQEIGPKMDWNTRETKQLIAGILELKNS
                     DEAKRFLRDLMTPQEIKEFANRLEAASLLSSLTQYNFITKRTGLSSATIARIAKWLNG
                     SLGGYRLILNRLNHHHNHSKLRKGLSLSS"
     gene            1965..2456
                     /locus_tag="VI981_03025"
     CDS             1965..2456
                     /locus_tag="VI981_03025"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479302.1"
                     /translation="MSRKLQIGVMGSAADLNYSETVAILAEEVGRELALQNCTVVFGA
                     EKDYDSLSTAAGRGARSAGGLTIGITYGKGLVVFDEADVVVTCGAERGGPRESVLVLS
                     CDAIICISGDSGTLTEMLVAYQSNIPIIALTGTGGWSDKIAGQFFDARKRQHNISPKM
                     LDF"
     gene            2621..3508
                     /gene="hisG"
                     /locus_tag="VI981_03030"
     CDS             2621..3508
                     /gene="hisG"
                     /locus_tag="VI981_03030"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY4479303.1"
                     /translation="MKPIDIVVPDGSMQEVVMGLFAKAGMPITIEKKRTKEGKVGVPW
                     IKRVAFQRPQEIPHYLHRGHFDIAICGEDWLANWGYKFPILLKLPIGRSGNKLVKVVL
                     AVSQTSGISRVEDLPLGAEVATEYVQLVEKFFKERGRTDIRVLPSFGNTEHKIAFGAT
                     AIVDVSESGDSIGENQLEAIDMVMESHTVIVANEDSLAEKEKRPYIDCFVRLIRGAYQ
                     ASQYVMVIANVPTNAVEEASRILGGLKGASRSPLAVPDWFALQSIVRREDEQTVIFKL
                     LQIGVTDIIVNRDIPLIMT"
     gene            complement(3732..>3973)
                     /locus_tag="VI981_03035"
     CDS             complement(3732..>3973)
                     /locus_tag="VI981_03035"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY4479304.1"
                     /translation="VPGWNLSEDGKKISKEFKFKDFIGAINFVERVADVAEMEDHHPD
                     IHVYYNKVVLELWTHAIGGLSENDFIVAAKVDAHN"
BASE COUNT         1139 a          918 c          929 g          987 t
ORIGIN      
        1 atttgaaaaa gcatagttat aaaaatacgg agacagttca cctctggcaa gcatttgaaa
       61 aggtctcgaa gaaaaaagtc gagcagatga tgcggacttg gacgagccga cccggctatc
      121 cagtgctgga agccaaactc ctaaataata aattattctt gtctcaaagc agattttttg
      181 caagccctgc atctaaacaa aaagccaaag ataaaacaat ctggcaaata ccagtatcaa
      241 tagaagacaa aaagggtcct tgggcaaaaa taaatattgg tgagacagga ttcttcagaa
      301 cggcttattc aaaggaacta ctggagaaac tatacgcccc agtcaaaggg aaaattttag
      361 aagctagcga ccggctcggt attgtgcggg atttgtttgc tctatcagag gcaggcacca
      421 tccccacgac cgatgctttg gactttttat ctgcttataa aagtgaggac aattatatgg
      481 tttgggtaga aattgccact ggcctaagta aaattgaaca acttgtaacc aaaacaccga
      541 tggaagaaga ttttaaaaaa ttcatgcatg acttgttttc gccgctggct ctgcgttttg
      601 gctggaataa aaagaaaaag gaattgcata gcgccacact tctgcgacca cttgtacttt
      661 cccgggccgg acttgccgga aataaaaaaa taataaaaga ggcaaaagag aaattcgcac
      721 tcttgcaaag gggcggctcc gtctctcctg atgtacgttc tagtgtctat acaatcctcg
      781 cgcgacacgg cggaacgaaa gaatacaaaa ttttaataaa aaaatataaa aaagaaacac
      841 tgcacgaaga aaaaaatcgc attggcaacg ccttgggatt ttttccaaat cctaaaattc
      901 ttgcatttgt ctgtaaattc tctctaagca aggacgtacg catgcaggac accattggca
      961 tcatctcctc cgtgggaaca aatcctgccg gccgggatgt ctggtggaat ttcgtgaaaa
     1021 agaattggaa attgctcgtc tctcgctacg gcgacggcgg gcatactctg gcgcgactag
     1081 taaaagcaat aagcggaagc ccggaggcaa aacatttgtt ttcctttaaa aagtttttca
     1141 aaacgcgcga cgcccccggc gccaagcgtt ctatagaaca agtcctggag cgcctcgaag
     1201 gcaacatctt gtggctaaaa cgtgacaaaa aacttatcca caccttcctg tctactttac
     1261 ttgactaaag ttgtgaattc tagcatatgc taaaattcac gacactaaaa ctcatgagtt
     1321 aactactgag ttatccacac ccccttgtta ctttagttga ctaaagtact ttagtttgct
     1381 aaagtagggg tacctgtccc gtactaaatt tttgattacg ggacataaca ataaacatta
     1441 ttagttaaaa ttttagtact gggatgagta aagggatgaa taaaaacaca acgataaaaa
     1501 cagttcggct gattcaggaa attggcccta aaatggactg gaataccaga gaaaccaagc
     1561 aactaattgc tgggatttta gagcttaaaa acagcgatga ggcgaaacgg tttttgcgtg
     1621 accttatgac tccgcaagaa ataaaagaat tcgccaatcg cctggaggct gcttccctgc
     1681 tctcatccct aacgcaatac aatttcatta cgaaaagaac tggcttgtcc tctgccacga
     1741 ttgctcgcat tgccaaatgg ctcaacggca gcttgggcgg ctaccgctta attcttaatc
     1801 gtttaaatca ccatcacaac cattccaaac ttaggaaagg gttgtctttg agctcgtaaa
     1861 atatttttgt ttgaagttaa cccctcccta ggttttacct agggaggggt tttgcgttgt
     1921 ggcgcaaaac ttagtcaact ttgttctttg agagggagag gcagatgtca agaaaactgc
     1981 aaatcggagt gatgggaagc gcggcagatc tgaactactc ggaaaccgta gccatcttgg
     2041 ccgaagaagt cggtcgcgag ttggcccttc aaaactgcac cgtcgttttt ggggccgaga
     2101 aagattacga ttcgctctcg acggccgctg gtcgcggcgc gcgtagcgcc ggaggtctca
     2161 caatcggcat cacatacggc aagggtctgg tggtttttga cgaagccgat gtggtggtga
     2221 cgtgcggcgc agagcgcggc ggtccgcgcg agtccgttct ggttctttcc tgtgatgcga
     2281 tcatctgcat ctccggagat agcggaacgc tcaccgagat gttggtcgcc taccaatcca
     2341 acattcccat catcgccttg accggcacgg gcggatggag tgataagata gccggacagt
     2401 tcttcgacgc tcgcaaacgg caacacaata taagcccaaa aatgcttgac ttttgacttg
     2461 aaacatgcta tactacaagg atatagcgtt ctttgaatat tttaggtttt ttggagccag
     2521 gcaattaggt tgccttaaaa aagttgctta atcgtctggt tcagtcggcc acgaccccga
     2581 aagaagccgt agctttggct ttgaaagcag tggaaaggaa atgaagccaa ttgacatcgt
     2641 cgtcccggac ggctcgatgc aagaagtcgt catgggtctg ttcgcgaaag cgggcatgcc
     2701 catcaccatc gagaagaagc gcaccaagga aggcaaagtc ggcgttcctt ggatcaaacg
     2761 ggtcgccttt cagcgaccgc aggaaattcc gcactatctt cacagggggc actttgacat
     2821 cgcaatctgt ggagaggact ggctcgcaaa ttggggctac aaattcccca tcttactaaa
     2881 gctgcccatc ggtcgcagcg gaaacaagct agtcaaagtc gtcttggcgg taagtcagac
     2941 cagtggcatc agccgggtcg aagacctgcc tctaggcgca gaggtggcca ccgagtacgt
     3001 ccagcttgtc gagaagttct tcaaagagcg gggtcgtaca gacatccgtg tcctgccgtc
     3061 atttggcaac acggaacaca agatcgcttt cggtgcgacg gcgatcgtcg acgtaagcga
     3121 aagtggagac tctatcggag agaaccaact tgaagccatc gacatggtca tggaatctca
     3181 caccgtgatc gtggcgaacg aggattcact ggcggagaag gagaagcgac cgtacatcga
     3241 ctgcttcgtg cgactcattc gcggcgcgta ccaggcctcg caatatgtga tggttattgc
     3301 gaacgtccca acgaacgcag tggaagaagc tagccgaatc ttgggaggat tgaagggggc
     3361 atctcgttcc cctcttgccg ttccggattg gttcgcgctt cagtccatcg tccgacgcga
     3421 agacgaacag acggtgattt tcaaactcct gcaaatcgga gtcactgaca tcatcgtgaa
     3481 ccgcgacatc ccgctcatca tgacttaaga taacgcttgc tatctacagg ccgagagtcc
     3541 tgatcaactc tcataaagcc agcaacactc tgaaaggaaa atgttcgctg gctttttatt
     3601 ttctaaatac ttcatttctt gctactttag ttgactaaag taatttagtt taataaagta
     3661 aatataatgt gttgttattt tgtgcatatg cataaattaa caacataatt ttatttccgt
     3721 ttttcaaaaa cttaattgtg cgcatccact tttgccgcga cgatgaagtc attctcggag
     3781 agccccccga tggcgtgcgt ccaaagttcc agaactactt tgttgtaata tacatgaata
     3841 tcaggatgat ggtcctccat ttccgctaca tccgccaccc gctccacgaa atttatcgct
     3901 ccgataaaat ccttgaactt aaattctttg gaaatctttt taccgtcttc gcttaaattc
     3961 cagcccggca cat
//
LOCUS       DATDSU010000092        14710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1750905, whole genome shotgun sequence.
ACCESSION   DATDSU010000092 DATDSU010000000
VERSION     DATDSU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14710
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750905"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VI981_03040"
     CDS             <1..413
                     /locus_tag="VI981_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479305.1"
                     /translation="DMAARVISTRPKYEVVEYVFYDGKPVSVNQRRVINFGTAIQLQA
                     TYPRGVVKDPLAAEYSFLGFTYRRRFYLLEDAFLEGTKRTQCPDPRPSPTAEVAPFQP
                     TNEEGTLLATIQQVLSLPQIARQKQQRQSELVVA"
     gene            complement(649..1773)
                     /gene="pilM"
                     /locus_tag="VI981_03045"
     CDS             complement(649..1773)
                     /gene="pilM"
                     /locus_tag="VI981_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY4479306.1"
                     /translation="MNPLTTISNLFRGGGASAVGIDFGSSSVKVVQLGKHAGHAVLET
                     YGELAIGPYGNAEIGNAVKLSVSKMGEALVDVLREAKISSRECGIAIPISSSLVTTIE
                     LPLVGEQQFAQMVPIEARKYIPVPITEVTLDWWVIPKETSGVTYPFGTHEGPDIKTGR
                     MVDVLLAVIHNDAVRNYQDLVKSVGLNSSFYEVEIFSTIRAVLTQELKPLMIFDMGAA
                     TTKLYIVDRGVVKSSHMINRGSQDITQSLSRALALSVKEAELLKRNMGLDTTSGRRDI
                     YELVSTTLAYICSEANRVVLNYEKRTGQSLSKVMLSGGGALLKEFHTFAKDFFETEVV
                     MADPFAKVESPAFLRNTLQRAGPEFSVAIGIALRKLQEMS"
     gene            1956..2621
                     /locus_tag="VI981_03050"
     CDS             1956..2621
                     /locus_tag="VI981_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY4479307.1"
                     /translation="MVALKTVLHRFARGFIDLLLPQAAEVRFLEDASVASLLQKARPA
                     SKELPGIISVFDYRDPLIKRMVWEIKFRGNRRLAKLAADVLYEHLLAELSELKLFSGG
                     SQVLLVPVPLAAKRMRGRGFNQAERIADEMIKIDGGKNFSISTSVVRVKETKSQTQTK
                     SRSERMQNMNGAFSVINKKSVMGKNIIVLDDVVTTGSTIGEIKKVLLQSGAASVIGLT
                     LAH"
     gene            2815..2903
                     /locus_tag="VI981_03055"
     tRNA            2815..2903
                     /locus_tag="VI981_03055"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2849..2851,aa:Ser,seq:gct)
     gene            complement(2979..3242)
                     /locus_tag="VI981_03060"
     CDS             complement(2979..3242)
                     /locus_tag="VI981_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479308.1"
                     /translation="MEFLRYFDTRKNRAQEFIRYQLSRGENWFVLEILQCKSFSADVA
                     TGELYREEFRDESAAEEAYRNKLSLAETLSPSVLIQKEIWNSK"
     gene            complement(3245..5380)
                     /locus_tag="VI981_03065"
     CDS             complement(3245..5380)
                     /locus_tag="VI981_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY4479309.1"
                     /translation="MLTREQRIQLFSQVFRGRQEVFARRWEKWDKSIAGYAPVYTDEH
                     KEAYQPLTPDWIEKHLLGTSTLGIYPLLQDNTCHFVVADFDSDGWQKAVQSFVGICTR
                     HNISLAIERSRSGNGAHAWCFFSDPYSAARARKIFLAVLREAKIIDEYDKDESYDRLF
                     PSQDFLSGKGLGNLIALPLQGIPRKDGNSVFVDVENDFTAYDDQWEFLASVTKLSTVE
                     LNALFAKFSNIPTTTISTKKNTEKNKGVLVLTLRNTLDIHRSAVPPQLISYVREHLNI
                     LNIDRLVKERAGLPTYGLQKYIKTLSVEDDSLHIPRGFQKNLRDWLKEKNIPCGFVDE
                     RPQFDQIEFSQHITLRSYQERAVDAFSNVENGVLVAPAASGKTLMALALIAQKRQPAI
                     ILTHRRQIYEQWRDQIESSFGIPKRKIGQISSTKKQALLPVAVAMVQTLSRMKDWKGL
                     EEKFGTVILDECHHVPARMFRDVLSKFNCRYLYGLTATPERKYNDGTLISVYIGPVVH
                     TVDKHETEKVRETETTVIAPHAEHRVRSIVSDLQVPFGDSLRHFPLIAKVISNDTNRN
                     SLITGDVAATTRDGKKCLILTERKEHVEMLQAYLRKEFEVVMYSGDLSSRARQFALQK
                     IKSGRFQILIATGQILGEGVDIAGLEVLFLAFPVSFHGKLAQYVGRIRREGGAKLIFD
                     YRDPRVPLLEKMWKKRLSYYRKEGFEIQE"
     gene            complement(5387..6439)
                     /locus_tag="VI981_03070"
     CDS             complement(5387..6439)
                     /locus_tag="VI981_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY4479310.1"
                     /translation="MNSRFNKRIRVTQAMLHKIARIDELKGLWRGSAKLNPHVLKQLK
                     ASVIITSTGASTRIEGSRMSDAEVERLLRSLKTKAPKGRDAEEVAGCADLLGRIFDNY
                     KTLKLTEGQVLQFHEILLHFSTKDKLEKGRYKSTDNIVIARGDKGEEIVLFRPTLPFL
                     VKKEMDDVLVWAVNAQQKKELHPLLITANFIFEFLAIHPFHDGNGRLSRALTNLLLLQ
                     SGYAYIPYVSLEEIIENQKDEYYLALRGAQRWHKTGHEDITTWVLFLLDVLLKQAEAA
                     QSLIESDDPEQLLSEGQIAILRLFEGDKTLSPKEVSALLKETPLPTIKQTLTRLVKLG
                     LIKRVGLGRATRYTKL"
     gene            6667..6822
                     /locus_tag="VI981_03075"
     CDS             6667..6822
                     /locus_tag="VI981_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479311.1"
                     /translation="MKTPPFTNITVFILFFGIALIEALQKQNWLEAALFLALGILSLW
                     ADFKKTS"
     gene            6843..8138
                     /locus_tag="VI981_03080"
     CDS             6843..8138
                     /locus_tag="VI981_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479312.1"
                     /translation="MSFETPPPASFEQEGNERKSIRERIVFPHVQPILEALEKQFEGT
                     DKKLDSEVVKDILYEFIEQRINRAGVYESSEQGASFPWGVTDTAELAKKLYSRYSGEV
                     LPEKLEPNPEKRKKEFMFTSLQLVQSGSQFAFMEEPLHQAVKDLPRALSMLKDGKEPE
                     ANEIYTLGSPTNELGSMSPEFLEKIKNGKVFDEFGKLYSEFIKSKIREKQEKDTEILL
                     YGQSMGASFATGTAKHLLDDESVTQSREAAEEKDTPFLQVRLDMPVGSADTPEKRKKW
                     QIPVGFALDTAYTMATDPYLRTAMANDKKFLSSTQEIFTERSMPPVMSEEQVALKKQG
                     IKEAINSLRAGTPIPEDLKVTNVVGLYDPLMYSGEFNKEMKAHKKEHRGSLGKSMVSK
                     SPGQRTFGINMHHTMPYLRENELNRFYQAAKSLKDLKQS"
     gene            8249..9028
                     /locus_tag="VI981_03085"
     CDS             8249..9028
                     /locus_tag="VI981_03085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4479313.1"
                     /translation="MVSPTEVMQGEPVIITIEGTNTLSSIKNISFNGTPLKIFLYQNK
                     PTALLGIDLRQTPGMYKITGKLSDGSILEKILVVDRREKIELPLGIPEKLGGNTPTSQ
                     TTLISTLATENAILASLRTGTHAFWREKFRFPLSATTTITDRYGYFRQTGAYTIAHKG
                     TDFRAAEGTNVFAMNRGVVRLAREFRNYGKTIVVDHGLGLMTFYMHLSRIKVNVGELV
                     LPGQLIALSGQTGYAEKPHLHLTVRIGGISIDPIKFTDFFK"
     gene            complement(9141..9707)
                     /locus_tag="VI981_03090"
     CDS             complement(9141..9707)
                     /locus_tag="VI981_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479314.1"
                     /translation="MQLTEVDHEQVRTARAANALVPVEKHRIQNPAGTILVPCPDGDQ
                     FRDVYGQHCRLCNIERHHPLSLNGGALLLSKHSPVRHAKLKGSALLLDIKETQGLKGM
                     DTLALYVHAPCGVAYGTSLDFFEVMRLLIEAKLRLLAQRTLAKLKIVCFCHVDYPSAT
                     SEVRKRTYFVNRAKTTEFLAANGREVRV"
     gene            9836..11986
                     /gene="recG"
                     /locus_tag="VI981_03095"
     CDS             9836..11986
                     /gene="recG"
                     /locus_tag="VI981_03095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY4479315.1"
                     /translation="METDIALEQILQIRPAQKQALKKLAIESAKDLLYHFPARYETTG
                     KAKTVVELSAGMQAVVFGRVSGLKTKKAFIKKIPMAEASLTDETGSIKIVWFHQPYLA
                     KMLGEGSLVRLEGKVTERKGTLYFSNPKVERVDKIPRATDNLFGKEADAHTLYPVYPE
                     TRGVTSNWIYYGVQKVIQTGILDELKDPLPEALRKKYHLPGLKTAFIWIHAPKNEADA
                     LSARKRFAFEEIFFIQLEKQQERARFAKEAGFSIAINENEFKDFVARFPFKPTDGQTR
                     AIEMIQKDFKKPHPMARLLEGDVGSGKTFVAAVTAHTVVTSRPAGQDFGNLQVAYMAP
                     TEVLARQQFESFIQYFAHIGINVGLITGSGCQKFPSKINPRATTDISRTQLLKWVANG
                     EIPILVGTHALIQKSVRFKHLAYVVIDEQHRFGTGQRQTLANKEAFKPHLLSMTATPI
                     PRTLALTIYGDLDLTVLDEMPAGRKPVITEIVSPERRKVVYEEIRKELAVGRQAYVIC
                     PRINEPDPSKELALLAKSVTEEAVRLKREIFPEYEIAIMHSKMKNEEKESVMNEFKSG
                     KIKILVSTSVVEVGVNVVNATIIIIEGAERFGLAQLHQLRGRVVRSTKQAFCYIFTET
                     KSKNSVARLKALQTAKNGFELAEHDLQFRGSGELYGRKQWGLSDVAMEALKNIKMVEA
                     ARTEAHELIEKDPELKKNSALAEKLSEKKEIHFE"
     gene            complement(12931..14193)
                     /locus_tag="VI981_03100"
     CDS             complement(12931..14193)
                     /locus_tag="VI981_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479316.1"
                     /translation="MNKYWLHITVGIVAILLVGIAIPKLTVHDLVPVETRLRQCATLG
                     VTQHLDNPLERIALFLGESRITRADNNNGIEVESFTIFRIPLGILRGQPDMKLGVFCS
                     PAGDPSGTQVMESPSFADGDTVGLGVFHDSPIANGVKAYQSERLGISFKYPPDYLLFE
                     GKGEGVGGVEYYVITVALEMYVREAIARIGGGEWPASMHLSFYREPTLAVSNIVPLDY
                     LENWIRTKSQSNFVPSDPAQEGILTPTEVAGIPALKYRVRGLYDSDYLAFTYGEWVVL
                     ASADDMGENTDRDFQVILDSIQVGPAHTYTNKSLGFSIDKPTGVYFIGEENGVVAFSL
                     LSPNDPRQASSIGLVNVLTITSARTTPEEGKESEINGVRVVVSSTYGAYGGEKHWSYF
                     FSDHDLLIQYVENKPIYESMISTIRFDE"
     gene            complement(14196..14399)
                     /locus_tag="VI981_03105"
     CDS             complement(14196..14399)
                     /locus_tag="VI981_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479317.1"
                     /translation="MLISKKLGENIKKIRTRKGMSQGDICRALDMDRGYMSAIENGKK
                     NITIQQLERLAQALGVSVDKLLK"
BASE COUNT         4108 a         3532 c         3311 g         3759 t
ORIGIN      
        1 atgacatggc ggcacgggtt atttcgacac ggccgaaata tgaagtcgta gaatacgtct
       61 tttacgacgg caagccagtg tcagtaaacc agcgccgcgt gattaacttc ggcaccgcaa
      121 tacagctaca agcgacatac ccacgtggag tggtgaaaga tccgctcgcg gccgagtaca
      181 gtttcctcgg attcacctat cgccggcggt tctacctcct ggaagacgca ttcctagaag
      241 gcacaaagag gacacaatgt cctgatccgc ggccaagccc gacagccgag gtggcgccct
      301 tccagccaac caatgaggaa ggaactctct tggcgacaat acaacaggtt ctctcgctac
      361 cccagatcgc aaggcaaaaa caacaaagac aatccgagct tgtggtagcg taacgaatca
      421 aaaaaacaaa aggcttcgcg cacacgcgaa gccttttctt tttttacatc aaaagcaggt
      481 tatgtaacct tccgcttcac tctaagcgag ggtgaacttt tgcagaacct tgcgtagcgt
      541 ggcggcgctc gccctaacaa gttttgcttc gcaaaatttg cgagggcgag cagagcaaaa
      601 atcaagcagg attttatgcg tgtttaaaaa tcctaacccg agcgacctct aggacatttc
      661 ttggagtttc ctaagcgcga tgccgatcgc cactgaaaat tcaggccccg cgcgctgtaa
      721 cgtatttcgg agaaatgccg gcgactcaac cttcgcgaag gggtcggcca tgaccacctc
      781 ggtctcaaag aaatctttgg cgaacgtatg aaattctttt agaagcgcgc ctccgccgga
      841 aagcatcacc ttgcttaagc tttgcccggt tcgcttctca taatttaaaa ccacacggtt
      901 tgcttccgaa caaatgtacg cgagtgtggt ggacacgagc tcatagatat cgcgacggcc
      961 actcgtcgta tcaagaccca tattcctttt taagagctcg gcctctttga cggaaagagc
     1021 gagcgcccga gagagcgact gcgtgatatc ttgcgatccg cgattgatca tgtgcgagct
     1081 tttgactacg cctcgatcaa caatatagag ctttgtcgtc gccgctccca tatcaaaaat
     1141 catcagcggt tttaattctt gggtaagcac cgcgcgaatc gtgctaaaaa tctccacttc
     1201 ataaaatgaa gaattgagac cgacagactt cactaaatcc tgataattac gaaccgcatc
     1261 gttgtgaatg acagccaaca gaacatccac catccgcccc gtttttatat cgggaccctc
     1321 atgcgtgcca aacggatagg tcacgccgct cgtctctttt ggaatgaccc accaatccaa
     1381 ggtgacttcg gtaatgggaa cgggaatata tttacgcgct tcaattggca ccatctgcgc
     1441 gaattgctgc tcacccacaa gcggcaactc aatcgtggta accaagcttg aagaaatagg
     1501 tatggcgata ccgcactcgc ggcttgaaat cttcgcctcg cgcaatacat ccacgagcgc
     1561 ttcccccatt ttgctcactg aaagcttgac cgcattgccg atttctgcgt tgccgtaggg
     1621 accaatggca agctccccat acgtctctag cacggcgtgc cccgcgtgct ttcccaattg
     1681 cacaactttc acggatgaag agccgaagtc aatacccact gcgcttgcgc cgccgcctct
     1741 aaagaggtta gatatggttg tcaggggatt catgatgtaa attcttgcca ataaatcaat
     1801 tacctcgggg cagagccccg aggtatgacc cgaggcttcg ccgcataata atcttggaaa
     1861 ccaacggttt ccaaactttc cttagcggaa ccgcgcggca gagccgcggg gaaatccgct
     1921 tcgcgtatta tagcattctg tcgtataact tatatatggt ggctttaaaa acagtcttac
     1981 atcgtttcgc gaggggtttt atagacctcc ttcttccgca agcggcggag gtccgctttc
     2041 ttgaggacgc aagcgttgca agcttgttgc aaaaggcgcg acctgcaagc aaagagctac
     2101 caggaataat ttctgtgttt gattatcgag atccgctgat aaagcggatg gtatgggaaa
     2161 taaaatttag agggaaccgg cggctcgcaa agctcgccgc cgacgttttg tacgaacacc
     2221 ttcttgccga gttaagcgaa ctgaaacttt ttagtggagg aagccaagta ctgctcgtcc
     2281 ccgttcccct cgccgcgaag cgcatgagag ggcgcggctt taaccaagcc gagcgtattg
     2341 cagatgagat gataaaaata gacggcggca aaaatttttc catatcaacg tcagtggtac
     2401 gcgtgaaaga aaccaaaagc caaacgcaaa caaaaagccg gagcgagcgc atgcaaaata
     2461 tgaacggggc gtttagcgtg ataaacaaaa aatcagtcat gggcaaaaat attattgtcc
     2521 ttgatgatgt cgtcaccacc ggtagcacta ttggcgaaat caaaaaagtt cttttgcaat
     2581 ccggcgccgc ttcggtgatt ggattaactc ttgcgcactg aaaaaagtgc gcgtgataca
     2641 aaatttaata tttcgtgtaa aatgtgatag atttaaaaac tctggagacg tggctatgaa
     2701 atatgaactg cttcatattt catccgattc cgcctttttg cggaaacagt ctataccccg
     2761 tttgggttgg ctgtagggag cctggcgaag ccgcgtcgac tgattgacaa tgaaggagac
     2821 gtggctgagt ggtcgaaagc gcctcactgc taacgaggta agccctttaa acggcttcat
     2881 gggttcgaat cccatcgtct ccgtattgac aaattagtat cggcgtgtcc ttgttgacta
     2941 agaagaaggg cgacccgagt gccctaaaca gaagcgcact acttcgaatt ccaaatttct
     3001 ttctggataa gtacgcttgg tgaaagtgtt tccgctaatg atagtttgtt tcgatatgcc
     3061 tcctctgcag cagactcatc cctgaactct tcacggtaaa gctcgcccgt agccacatca
     3121 gccgaaaatg atttgcattg caaaatttcc aagacaaacc aattctcacc tcttgatagt
     3181 tggtacctga taaactcttg cgccctattt ttacgcgtat caaaatacct gaggaattcc
     3241 atagttattc ttgtatttca aatccttcct ttcgatagta cgacaatcgt ttcttccaca
     3301 ttttctctag taacggtaca cgcggatctc ggtaatcgaa tataagtttt gctccacctt
     3361 cacgcctgat tcgccccaca tactgtgcaa gttttccatg gaatgaaacg ggaaaagcta
     3421 gaaataacac ttccaaccct gcaatatcca ctccttcgcc tagaatttgc ccggtggcga
     3481 tgagtatctg gaagcgccca ctttttatct tttgaagcgc aaattgccgc gcacgagaag
     3541 atagatctcc tgaatacata acgacttcaa attctttgcg aagatatgct tgcagcattt
     3601 ccacgtgctc ctttctctcg gtaagaatga ggcatttctt cccgtcgcgt gttgtagccg
     3661 ctacatcacc tgtaatgagt gaattcctat tcgtgtcatt cgaaatcact ttagcgatca
     3721 atggaaagtg cctcaaggag tcgccaaacg gaacctgtaa gtccgaaact atagatcgca
     3781 ctcggtgctc ggcatgtggt gcaataaccg ttgtttctgt ttctcgcact ttttctgttt
     3841 catgtttatc aacagtatgg acaactggtc caatgtaaac ggatataagt gtcccatcgt
     3901 tatattttct ttcaggtgtc gcagtaagtc catacagata tctgcaatta aatttcgaaa
     3961 gaacatcacg gaacatgcgc gcgggtacat ggtgacattc atcaagaatg acggtgccaa
     4021 atttctcctc aagccctttc cagtccttca tacgactcag cgtttgcacc atagcaacgg
     4081 ccactggcag aagtgcctgt ttctttgttg atgatatttg cccaatcttg cgtttgggta
     4141 taccaaaact gctttcaatt tgatctcgcc attgttcgta gatctgtcgc cgatgcgtga
     4201 ggattattgc cggctgtctt ttttgtgcaa taagagcaag agccataagt gtctttcccg
     4261 atgcggcagg tgccaccaga acaccattct cgacattcga aaatgcatcc acagctcgct
     4321 cttgatagga acgtagagta atatgctgag aaaattcgat ctgatcgaat tgtggtcgtt
     4381 catcgacaaa cccgcaagga atatttttct ctttcaacca atctctaaga tttttttgaa
     4441 atcctcgagg aatatggagc gaatcgtcct cgacagaaag tgtttttatg tatttctgta
     4501 gcccataggt gggtaaacct gctcgttctt ttaccaaccg atcaatgttg agaatattga
     4561 gatgttcgcg cacatatgag ataagttgtg gtggaactgc gcttcgatga atgtctagcg
     4621 tgtttcgaag cgtcagaact aacaccccct tgttcttctc ggtattcttt tttgttgaga
     4681 tggtcgttgt tggaatattc gaaaactttg caaaaagcgc attcaactcc actgtcgaca
     4741 gttttgtaac tgatgcaaga aactcccact gatcatcgta agcagtaaag tcgttttcta
     4801 catcgacaaa tacagaattt ccgtctttgc gaggaatgcc ttgaagcggc agtgctatca
     4861 aattacccag ccccttgcca gataaaaaat cctggcttgg aaacaaacga tcataacttt
     4921 cgtctttgtc atattcatct attattttcg cttcgcgcaa aacggcaaga aaaatcttcc
     4981 tcgctcgtgc ggcagaatac ggatcgctga aaaaacacca ggcatgcgcg ccgtttcctg
     5041 aacgggaacg ttcaatcgcg agtgatatgt tatgtctggt gcaaatgcca acgaaggact
     5101 gtaccgcctt ctgccaccca tcactatcaa aatccgcaac gacaaaatga cacgtattat
     5161 cttgaaggag tgggtatatg ccgagtgtgc tcgtgcctag gagatgcttt tcaatccaat
     5221 caggcgtcag tggttgatac gcctctttat gctcatccgt gtatacgggc gcgtatcctg
     5281 caatcgactt gtcccatttc tcccaacgcc tggcgaacac ttcttgtcgt ccgcgaaata
     5341 cttgactgaa tagctggata cgttgttcgc gtgtcaacat atgtacctat agttttgtgt
     5401 accgcgtcgc acgaccgagc ccaacgcgtt taatgagacc aagctttacg agacgcgtca
     5461 gcgtttgctt gatggtagga agaggagtct ctttaagcaa cgcggacacc tctttcgggg
     5521 aaagggtttt gtctccttca aaaagtcgta agattgcaat ctgtccctcc gaaagcagct
     5581 gctcaggatc gtcactttca atgagacttt gcgccgcttc ggcttgtttc aacaacacgt
     5641 caagcaaaaa cagaacccag gtcgttatgt cttcgtgccc cgtcttgtgc catcgctgcg
     5701 cgccacgaag cgcaagataa tattcgtctt tttggttttc gattatttct tccagagaaa
     5761 catatggtat gtacgcgtat cctgattgaa gtaatagcag gttcgtgagg gcgcgagaaa
     5821 gtcgaccatt gccgtcgtgg aacgggtgaa tcgctaaaaa ttcaaagatg aaattagccg
     5881 ttatcaaaag cggatgcagt tccttttttt gttgtgcgtt taccgcccac acaagcacgt
     5941 cgtccatttc tttctttacc aggaatggaa gtgttggcct gaaaagcaca atttcttcac
     6001 ctttgtctcc tcgtgcgata acaatattat ccgttgactt gtacctgcct ttttcgagct
     6061 tgtctttcgt cgagaaatga aggagaattt catgaaattg taaaacctgt ccttcggtga
     6121 gttttagtgt tttataatta tcgaatattc gtccgagtaa atcagcacac ccggcgactt
     6181 cttctgcatc acgtcctttt ggtgcttttg ttttgagact acggagcagg cgttcaactt
     6241 cagcgtcgct catgcgtgac ccttcgattc gagtcgacgc accggttgat gtaattatca
     6301 ctgatgcttt tagctgtttt agtacgtgag gattcaattt agcactacca cgccagaggc
     6361 ctttcagctc gtcgatgcgg gctattttgt ggagcatcgc ctgagtgact cgtattcttt
     6421 tattgaatcg ggaattcata tataatcaac acctgcgatt atatacgatt acattcgatt
     6481 gtcaagtcta aaaaggaaag ctatgacttt gttgagagct ctcaacgagg atattgggtg
     6541 ttaagcgtac cgtataattt gtcgcaaatt cttttttcgt ctgttgtttc gggaaccatt
     6601 atcgcatttg ctttgtcgcc gcagaaaaag aatggttaaa taattattat ttttagacaa
     6661 aaaatcatga aaaccccacc attcacaaac atcaccgtat ttatactctt ttttggaatc
     6721 gcgcttattg aagcattgca gaaacaaaac tggcttgagg ccgcgctctt cctcgcactc
     6781 ggaattctct cgctctgggc tgattttaaa aaaactagtt aaaaagagag tataatcaag
     6841 gtatgagctt tgaaactccg ccaccggcta gctttgagca agaaggaaac gaacggaaat
     6901 ctatccgtga gagaatcgta ttcccgcatg tacaacccat actagaagcc cttgaaaaac
     6961 agtttgaggg gaccgacaaa aaacttgatt cggaagttgt aaaagacatc ctttatgaat
     7021 ttatagagca gagaataaat cgcgcgggag tctacgaatc cagcgaacaa ggagcttcat
     7081 tcccgtgggg agtgaccgat accgcagagc ttgcgaaaaa gctttacagc cgctatagcg
     7141 gagaagtgtt gccagagaaa ctagaaccaa accccgaaaa aagaaagaag gaatttatgt
     7201 tcacctcctt gcaacttgta cagagcggca gtcagttcgc gtttatggaa gaacccctac
     7261 accaggccgt caaagacttg cctcgtgcgc tttctatgct taaagatggc aaagagcctg
     7321 aagcaaacga aatttacacg ctcggttctc cgacaaatga actcggaagc atgtcgccag
     7381 agtttttaga aaaaataaag aacggcaaag tgtttgatga attcggaaaa ctatatagcg
     7441 aatttattaa atctaaaatt cgcgaaaagc aagaaaaaga tacggaaatt cttttgtatg
     7501 ggcaatccat gggcgcaagt tttgcaacag ggactgccaa acatttatta gacgacgaga
     7561 gcgtaaccca atcgcgcgag gcagcggaag aaaaagatac gccatttcta caagtacggc
     7621 ttgatatgcc tgtgggttct gccgataccc cagagaaaag aaaaaaatgg caaatccctg
     7681 taggctttgc gttagacaca gcctacacga tggcgacaga cccctacttg cgtaccgcga
     7741 tggccaacga taaaaaattc ctctcctcca cccaagaaat atttactgaa agaagtatgc
     7801 ctccagtgat gtcagaggaa caagtcgcac tgaaaaaaca agggatcaaa gaggctatca
     7861 atagcctgcg agcaggaacg ccaattcctg aagatcttaa agtaacaaac gtggtaggcc
     7921 tctacgatcc acttatgtat tcaggtgagt tcaacaaaga aatgaaagcg cacaaaaaag
     7981 aacaccgcgg gtcgcttggc aagagcatgg tatcaaaatc ccccggtcaa agaacgttcg
     8041 ggatcaatat gcaccatacg atgccgtatt taagagagaa cgaattgaac cgtttctatc
     8101 aggccgcaaa aagtttgaaa gacctaaaac aatcgtaaat agagagtata atcagggagt
     8161 gaaaaacctc cttataaaat cagttttcag tatagcggta ctgttatcat taccgagtat
     8221 tgtatgggcg gcatccactg tctcccttgt ggtctctcca acagaagtaa tgcaaggcga
     8281 gccggtaata atcactattg aaggcacgaa cacactctca agcataaaaa atatttcttt
     8341 taatgggaca ccactaaaaa tttttctata tcaaaacaaa ccgactgctt tgttgggaat
     8401 agatttgcgt cagacgcctg gcatgtacaa aataaccgga aaactttctg atggtagtat
     8461 tcttgaaaaa atactcgtcg tggatagacg ggaaaagatt gagcttccgc tcggaattcc
     8521 ggagaaactt ggcggcaata cgcctacaag tcaaaccacg ctcatcagca ccctggccac
     8581 tgaaaatgcg atccttgcaa gcctccgaac cggaacacat gcattttgga gagaaaaatt
     8641 ccgcttccca ctttcagcaa cgacaaccat taccgatagg tacggatact tccgtcaaac
     8701 cggggcgtac acgatagcgc acaaaggcac cgatttcaga gcagccgaag gaacaaacgt
     8761 gtttgcgatg aatcgcggcg tggtgcgact cgcgagagaa tttagaaact atggaaagac
     8821 tatcgttgta gatcatggcc tgggcctgat gacgttttac atgcatctct cgcgcatcaa
     8881 agtaaatgtc ggtgaattag ttctgcccgg acaacttatc gcactctcgg gccaaacagg
     8941 ctatgcagaa aaacctcact tgcatctgac ggtccgcatc ggcggaattt ccattgaccc
     9001 gataaagttc acggatttct ttaagtaggg aaacacggaa cattatttaa ttaagctata
     9061 gcttaattaa ataatgttct ctttttcaaa aaaatccgcc agagtgtaaa caccccagcg
     9121 gtttgcataa tccgataaca tcaaacacga acttcacgcc cgttggctgc taaaaactcc
     9181 gtcgtcttcg cacgattcac aaagtacgtg cgctttctca cttcgctggt agccgaagga
     9241 tagtcaacat ggcaaaaaca cacgattttg agcttcgcca gtgtcctctg agcgaggaga
     9301 cgaagtttag cttcgatgag caggcgcatc acctcaaaaa aatcgaggct agtaccataa
     9361 gcgactccgc atggcgcatg aacatacagc gcaagtgtat ccattccctt gagcccctgt
     9421 gtctctttga tatccagcaa gagggcagag ccttttaatt ttgcgtgccg aacgggcgaa
     9481 tgtttagaca agaggagcgc tcccccatta agagagagcg gatgatgccg ctcaatattg
     9541 cacagcctgc agtgctgtcc gtacacgtct cggaattggt ctccgtcagg acatggcaca
     9601 agaatcgtgc cagcgggatt ttgtatccga tgcttttcaa ctggcactag tgcatttgca
     9661 gctcgagctg tacgaacctg ttcatggtcg acttcggtta attgcatagg taggtttcct
     9721 tttgttggag tttaagtttg ggaaaaatga cgaaatacat ctgaaaatac actacattaa
     9781 tttaagattt tgtcaaactg cccgaaaaac tcccatttcg gctacaatag gccttatgga
     9841 gactgatata gcgcttgaac aaattcttca aatccgcccc gcgcaaaagc aagcgctcaa
     9901 aaaacttgcc atagaaagcg ctaaagacct tttgtatcac ttccccgccc gctacgaaac
     9961 gaccggaaaa gcaaaaacag tggtggaact ttctgcaggc atgcaagcgg tagtctttgg
    10021 gcgcgtcagc ggcctcaaaa ctaaaaaagc gttcattaaa aagatcccga tggcagaagc
    10081 gtcacttacc gatgaaacgg gaagtataaa aatcgtttgg ttccatcagc cctatctggc
    10141 taaaatgctt ggcgaaggga gtctggtgcg gttggaaggc aaggttacgg agcgaaaagg
    10201 cacgctgtac ttttccaacc cgaaagtaga acgcgtagat aaaatccctc gagcgactga
    10261 taacctcttt ggtaaagaag cagacgcgca cacgctctac cccgtttatc ccgagacgcg
    10321 tggcgttacg tcaaactgga tctactacgg cgtacagaaa gtaatacaaa cgggcatctt
    10381 ggacgaactg aaagaccctt tgcccgaagc actgcgaaag aaatatcacc tgccaggcct
    10441 caaaactgcg ttcatctgga ttcacgcgcc caaaaatgag gctgacgcgc tttccgcacg
    10501 caaacgattc gcttttgaag aaatattttt catacaactt gaaaagcaac aggaacgggc
    10561 gcgttttgca aaagaagctg gtttctcaat cgccataaac gaaaacgaat tcaaagattt
    10621 tgtagcgcgt ttccccttca aacctactga cggccaaacg cgcgcgatag aaatgataca
    10681 aaaagatttc aaaaaaccac acccaatggc gcgactttta gaaggtgatg tcggttcagg
    10741 aaaaacattc gtcgccgcag tcacggcgca cacagtcgtc acaagcagac ctgccggtca
    10801 ggattttggg aatctccagg tagcatacat ggcaccgacc gaagtcttgg cacggcaaca
    10861 gtttgaatcg ttcatacaat actttgcgca catcggcatc aacgtgggac tcattaccgg
    10921 aagtggttgc caaaaatttc cctcaaaaat aaacccgcgc gccacaacgg atatttcccg
    10981 aacgcaacta ctgaagtggg tggcaaacgg ggagatacca atactcgtag gcacgcacgc
    11041 gcttattcaa aaaagtgtgc gctttaaaca cctcgcgtac gtcgttattg atgagcaaca
    11101 tcgttttggt acgggacaac gccaaaccct cgcaaataaa gaagccttta agccccatct
    11161 gctttcaatg accgcgaccc caatcccccg cacccttgcg cttaccatct acggcgacct
    11221 tgatctgacg gtgttggatg aaatgccggc ggggcgtaaa ccagtcatca ctgaaattgt
    11281 ctcgccagag cgaagaaaag tagtctatga agaaatcaga aaagaactcg ccgtgggcag
    11341 gcaggcctac gtcatttgcc cgcgcattaa tgaaccagac ccaagcaaag aactggcact
    11401 ccttgcgaaa tcggtaacgg aagaggcggt gcgcctcaaa agagaaatat ttcccgagta
    11461 cgagattgcc atcatgcaca gcaagatgaa aaacgaggaa aaagaatcgg tgatgaacga
    11521 attcaagtcc ggaaaaataa aaattcttgt ctccacctct gtggttgaag tgggagtcaa
    11581 tgtagtcaat gcaactatta tcattattga aggagccgag cgttttggtc tagcgcaact
    11641 gcaccagttg cgcgggcgtg tggtgcgtag cacaaaacaa gctttttgct atatattcac
    11701 cgaaacgaaa tcaaaaaatt ctgttgcacg cctcaaagcc ctgcaaacgg caaaaaatgg
    11761 atttgaactt gcggagcacg acctgcaatt tcggggttct ggggaactgt acggaaggaa
    11821 acaatggggt ctctcggatg tcgctatgga agcactaaaa aatataaaaa tggtggaagc
    11881 ggcgcgaacc gaagcacatg aattgataga gaaagatcct gaacttaaaa aaaattcggc
    11941 cctcgcagaa aaactttcag aaaaaaaaga gatccatttc gagtaaaggt gactcaaata
    12001 acagaatcaa aaccactctt caacactaaa aagtgtaccc cccccccacg aaggtttctg
    12061 tcaaaatcaa aagtttataa tttatgcgcg ttcagtgagc tttctctctc gagtactcta
    12121 atcatacact ttgcgcgaac ttatttcaca cgcgcggagc gcgtggtggc ttgaaattga
    12181 atcgtacgct ccaatctgcg cgcctcgtcc gctcccttcg gtcgcgggca aaaaccaaaa
    12241 attttccttc cttatttcag attttcggcg gggggtgtgg ggggagccga caaaaaatgg
    12301 aaaggaaatt tttggttttg cttcgccgct tcagactact aaaagagcaa tcttatttta
    12361 acatttttgt ttgctctttt ctccgtcctt gcggcgagac taggcgcttc ctctcggcgt
    12421 tctgtggtaa ctctaaactc tgtggcttcg ataactggta gcggtaaatc aaaatatgga
    12481 ttgtgctagg cacaaacaaa tctgtatgtg cttgccccgc ccgccctgtg cgcgcacaaa
    12541 tccccgccgt ttttaggaaa cattataaaa cggcggggat tgctcccttg aattcgaatt
    12601 attttataag ctgacaacta ttgttaatac aatcaactcc tttgaactca atacacaaat
    12661 attgacactt gctttcgtag ctttcgagca atttaccaat tcgtgtcgct tcgtttttgt
    12721 tcacatgaac ataacagcca aatggacatt gtgattgggc gaccatttgg caatctgata
    12781 caacttcaca atagttcgct ttggtaattt cttttttgat atgccgctct tgcgctttag
    12841 gactgaaaaa agaaaaccac gccaacaagc agaattacga agaccagtat cgttctcttg
    12901 atttttgtat ccgttttcat atgggacata ttattcgtca aagcgaatgg tgctgatcat
    12961 actctcgtag attggcttgt tctctacata ctggataagc aggtcgtgat cggagaagaa
    13021 gtaactccag tgcttctctc ctccatacgc tccgtaggta ctcgatacca ccactcttac
    13081 cccgtttatt tccgattctt tgccttcttc aggtgtggtt cgtgctgagg taatcgtcag
    13141 cacatttacg agtcctatgc ttgatgcctg tcgaggatca ttgggagaca gcaaagaaaa
    13201 cgcgaccacg ccgttttcct cgccaatgaa atatacgcca gtcggtttat cgatagaaaa
    13261 accgagagac ttgttggtat aagtgtgcgc tgggcctacc tgtatcgaat cgagaataac
    13321 ctgaaaatct cgatcggtgt tctctcccat gtcgtctgca ctcgcaagca ctacccactc
    13381 tccgtaggtg aaggcgaggt agtcgctgtc gtagagacca cgcacgcgat atttgagggc
    13441 aggtataccg gcgacctcgg taggagtgag gatgccttcc tgcgccggat cggaagggac
    13501 aaagtttgat tgagacttgg tgcgtatcca attttcaaga taatcgagcg gtacgatgtt
    13561 cgacacggca agagttggtt cgcggtagaa ggaaaggtgc attgacgcag gccactcgcc
    13621 tccgccgatc cgcgcaatcg cttcgcgcac atacatctcc agagcgaccg tgatcacgta
    13681 atactccact ccgccgacgc cttcgccctt gccttcgaac agaagatagt cgggtggata
    13741 tttgaacgag atgccgagtc gctctgactg gtacgccttt acgccgtttg caatcggcga
    13801 gtcgtggaat actccaagcc ctaccgtatc tccatcggcg aatgatggcg actccatgac
    13861 ttgtgtgcca cttggatcgc ctgccggact gcaaaagacg ccgagcttca tatcgggctg
    13921 tccacgaagg atgccgagcg gtatacggaa aatcgtaaac gactccactt caattccgtt
    13981 attattatct gcccgtgtga tgcgactctc acccaagaaa agtgcaatgc gttcaagtgg
    14041 attgtcgagg tgttgagtga cccccaaggt agcgcattgc ctcaatctgg tttcaactgg
    14101 cacgagatca tggaccgtga gtttcggtat cgcgatacca actagcaata ttgctacgat
    14161 accgacggta atatgtagcc aatatttatt catatttatt tgagcaattt atcaaccgaa
    14221 acgccgagcg cttgcgccaa tctttctaat tgttgaatgg taatattttt cttcccattt
    14281 tcaatggcac tcatataccc tctatccata tcaagtgctc ggcaaatgtc gccttgcgac
    14341 attcccttac gagtccgtat tttcttaatg ttttctccta atttcttact tatcaacata
    14401 agtatagcat acgagatatt gcactttcaa acaatcttgt actataattc aagtggtagt
    14461 ggcgaagcaa aaccaaaaat ttcctttcca ttttttgtcg gctccccccc acaccccccg
    14521 ccgaaaatct gaaataagga aggaaaattt ttggtttttg ctcccgcgac caaagggagc
    14581 ggacgaggcg cgcagattag tccgtttgcc gttcgaaaaa agttcgagca aagtgtatga
    14641 ttagagcacc agatagaaaa atctcaactg cttgagattt ttcgatactg gtgcgagccg
    14701 cagtcatgtt
//
LOCUS       DATDSU010000093         8415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2103879, whole genome shotgun sequence.
ACCESSION   DATDSU010000093 DATDSU010000000
VERSION     DATDSU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2103879"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1136)
                     /locus_tag="VI981_03110"
     CDS             complement(291..1136)
                     /locus_tag="VI981_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479318.1"
                     /translation="MKKISKFCKLVWKKISEGIVSFLIDRAFSYIVGLIAGGTFFSVV
                     SGLVSYFNPALWPWWCTILIVYIPQQAAVYLYIFRDWLIEKEKKEQSKMYNPGISIEN
                     EDDEYEIFKDRRCLRKNTVVIKALQDGVSQFRRSSLNTGARIVKIKIEEGGQLFESDE
                     EADELAWFVAFAPLPKGDTHRIVFRMEVENFQSFLRKHLASTFSTVKLRVVFAKKLII
                     DDEVSLSIKDGTTGKPLIKITDKKVKLNDCTDGSKKEAIWEFGTGKDYPSIADGVYLM
                     SWKWR"
     gene            1302..1523
                     /locus_tag="VI981_03115"
     CDS             1302..1523
                     /locus_tag="VI981_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479319.1"
                     /translation="MVKSVHTKEYAYFVGRLRKARLEARLTQVQVAKKLKRPQSHVSN
                     VESGQQRVDVIELQRFAKLYDKDIKYFVK"
     gene            1531..2028
                     /locus_tag="VI981_03120"
     CDS             1531..2028
                     /locus_tag="VI981_03120"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY4479320.1"
                     /translation="MNCQKCGNKIKEGDEFCQSCGVPVVKVAKGVRKLLRGRVGRKYF
                     FLGLFSLMLAFAVILGIVFICFALLASILPDGIALVIRWFGGALILLCSLIALSFVVS
                     LYVRRIHDIGKSAWWLLLLLVPFLNYVVVFYLLFKKGEASRNRFGDEPLSEAGLMDTI
                     FNRQP"
     gene            2067..3758
                     /locus_tag="VI981_03125"
     CDS             2067..3758
                     /locus_tag="VI981_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479321.1"
                     /translation="METKTLPAVAFFLTLFLATSIVLAAPTAPSKSKTPNIKTLSPNT
                     GGEGEVITMAGSNFGKDPADGIIGLNDGPNYVRVGGFLGTDMISWANNKVQIFAPSLQ
                     DAASNLSRMGIFFTVADSIGKNIPGFNLFLYTMKLFDAVTTQKSTFVDNTAPVTVVVY
                     PLNSSNTEYFTYKAKTYSPGIKGLAEKVIDATDAVHVAKITYDSFGKDIVGLVTNPVQ
                     LVKDKLRDNARSAFETPLLIQDAFSGFKSSEITPSPPEANIVAPTQKQPSPIGEGQKQ
                     PSKETKKDTTTTKVTEQKPPTEQKATRLTIKLVDQYDLPITEVNESEYCRTKLPSEVE
                     RAGACHELGTSPTLLLIDSVGDKVPGFFARFDKNEDFFYGNSSPTSPRGEWTYDRDIV
                     PGKYTLVFYKGGSTENSFTRRFYNSSEKQVSVSNSEGWFVKMTVSQIITHVFFEIHLK
                     IVNQAGTPMDPGGYYNQAGDYVAGFVMKDKNGEDVRIDAKAVENGQYVIRGVSTWLGN
                     WSYPPGDYILRVEKEGYAPVEKTIQIPDLSRISDDEIKLKRQDKIDLGTITLTKI"
     gene            3900..4454
                     /locus_tag="VI981_03130"
     CDS             3900..4454
                     /locus_tag="VI981_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479322.1"
                     /translation="MTKNQIIGVVAVVAVVVGAFFFGTYSSNKASRDGETDYDVALDK
                     AASIVSASLDAVRSEASAKAHDARRITDLKQLELALELYYGSNESYPLSIGRGTIGSL
                     AGAGYISNVPTDPVSNVTYIYAPLAKAGAATAAAICSSTQPCVSYVLLATLENSNTIP
                     NTSIKTNPLGGQNCRTTGVYCVVP"
     gene            4492..5148
                     /locus_tag="VI981_03135"
     CDS             4492..5148
                     /locus_tag="VI981_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY4479323.1"
                     /translation="MHRICKNCGSSNSEHARFCTKCGGALHSHEHAHERTPAEQAADV
                     SEKAKVEANKVIEKTKHIWSGFSKAEKIIAIGAIAGLAAFVLPWVSAGGQSVSGFSAA
                     SNSGYVYLLPLLMVASLLLLYFTQGASDTRKVLMTRWQIIIGSVTSTVGLFMIIFIST
                     IGSLMSQMMGGIGALFGGGSFGVSAGIGVYLYAAGAIAVVVGAFRLQGEVLQRLRDKD
                     "
     gene            5182..5442
                     /locus_tag="VI981_03140"
     CDS             5182..5442
                     /locus_tag="VI981_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479324.1"
                     /translation="MSDPTTSLLGGMFAFYGFTMLLFLVLGIVTFVFWINMLIKAIKN
                     EYEHKTLWILAIIFGQAVGAILFYFIVYKEKIKPQVAKVVSQ"
     gene            5468..5713
                     /locus_tag="VI981_03145"
     CDS             5468..5713
                     /locus_tag="VI981_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479325.1"
                     /translation="MTNSKLVGAGVLIVVGLVVITAGSMALPLLSRGDSYTYKVVERC
                     DWNFHPFEDQLNQTAKDGWEFIAGIGANNCHLVFRKK"
     gene            complement(5722..7218)
                     /locus_tag="VI981_03150"
     CDS             complement(5722..7218)
                     /locus_tag="VI981_03150"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEY4479326.1"
                     /translation="MSGKLEKIKAFFERLFWGENLLNASNKEEATQILQREPTPDELV
                     LGWAEGTDETTKQTTILPKGVSQEHRSTHFYVVGASGSGKTKFLETLAEQDIKNGHGF
                     GVIDAHGDLTEDLKGYLYLLKKDEPEFLRERVVLIDPTDPASTTCFNPLEKTEGITPA
                     GIASELVEVFKKIWKDSWGARMEDLLKNTLIALIENNLTLAELPLFLTDFNFRKFVLE
                     KVEHPICRQYFERYNTLAPHTRNEWIESTLNKVNAFLSDDNVRQMFVSPKSTFNLRDV
                     MDNQKVLLVKLDRGHLKGNADLLGSLLLAKLQMAAFSRTDIPASKRVPFYLYIDEFQN
                     FATESFAQTLAEARKYKLSLILAHQNLAQLLPPLRASILSNCGLQAYFRISRDDSNVL
                     AKESLSSIYNDPPGWEWYIRQLQELPHRACVVKNKIEGGVIAISTLNLAPPYETADMD
                     EEEFRAEVATFRIGGSHLRPRENVEKEYKTRREELIKSAEQESFRESK"
     gene            complement(7225..7914)
                     /locus_tag="VI981_03155"
     CDS             complement(7225..7914)
                     /locus_tag="VI981_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479327.1"
                     /translation="MWTFIIIVVIGVIVYLAYKNKQEGGLKKEKLKGGNIVDGMFGFT
                     KTIKQEKIIDRWSTLIDGANGQGEQVIAETIRIIEKLEAPNIFVSRQKRKPGPGFTKT
                     PREFLIAEHRILDGYDMYIGARDYGKQLFVSWCLIEEPISFWRLFKRNPILAIFKLPL
                     LIFGQAIAKMQGGSGQFMSLLNLFDTEELTAYTTTVHHALLDSVKVVMENQKLDFTGI
                     EKRTKGFLNIA"
     gene            complement(8064..>8415)
                     /locus_tag="VI981_03160"
     CDS             complement(8064..>8415)
                     /locus_tag="VI981_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEY4479328.1"
                     /translation="DIKLARWVQGNDLKSALAKRGANPPLVPDAFFVLETPTHKYPCF
                     LEADRATMTQERYVNKLKMYWRHNREESFKDTLGVSNFRVLTITPNEGRSANLCRSAK
                     DADDRREGSLMRAV"
BASE COUNT         2244 a         1904 c         1952 g         2315 t
ORIGIN      
        1 cccgaagcac cgacattggc tgtgggcttg ggggtaatga atctgatatg aaggatagga
       61 tattcggtct agagatgtca aactacagag gcaataatgc ctcagactcg ttctattcaa
      121 gaaatcaccg ctatcttttc agatgaccta gcgagactta aatacaacaa caaaaaccgc
      181 tcctttttat tccatctctc ccatacgatt acattataac aaacctgatt tttaagttca
      241 atactgctgt atacatatcc acggagtcct tctcggtctt ataaatggtt tcatctccac
      301 ttccaagaca ttaaatatac cccatcagct attgatgggt aatccttgcc agtgccaaac
      361 tcccatattg cttctttttt cgacccatcg gtgcagtcgt ttaattttac ttttttgtct
      421 gtaatcttaa taagcggctt gcccgttgtg ccgtctttta tactcaaact aacctcatcg
      481 tctataataa gttttttagc gaaaaccact ctcaacttaa ctgtgctgaa cgtggacgcg
      541 agatgtttcc gtaaaaagga ctgaaaattc tccacctcca ttcgaaatac tatcctatgc
      601 gtgtcgcctt taggaagtgg ggcaaacgct acgaaccaag cgagttcgtc cgcctcctca
      661 tcactttcga atagctggcc tccttcctct atttttatct tgacgatccg cgcccccgta
      721 tttagagagc ttcgtcgaaa ttgagacacg ccgtcttgaa gagctttaat aaccacagta
      781 tttttccgca agcatcttct atctttgaaa atctcatact cgtcatcttc attctctatg
      841 gaaatccccg gattatacat ttttgattgt tcttttttct ctttttcaat cagccaatct
      901 ctgaaaatgt acaaatatac tgctgcttgt tggggaatat atacgataag tatcgtacac
      961 caccacggcc acagtgccgg attgaagtaa ctgactaaac cagaaacaac agaaaaaaat
     1021 gttcctcctg ctatgaggcc gacgatatag gagaacgctc ggtcaatgag aaaagagact
     1081 atcccttcgg atattttttt ccaaactaat ttacagaatt ttgagatttt tttcatatgt
     1141 ttttgcggga ttttctgtag ctcccacagc cccatactgc gggtcgtttt gtcttgcgtc
     1201 aaggatattt tttggctttg atttccttcc acacaataac tagctcatct atagatatcc
     1261 agactttgga tattgcttat ttggatagac catattacta tatggttaag tccgtacata
     1321 caaaggaata tgcgtatttt gtcggacgat tacgaaaagc aagacttgag gcaaggctaa
     1381 cgcaggttca agtcgctaag aagctcaaac gtccgcaatc acatgtttca aacgtggaat
     1441 cgggacagca aagagttgat gtgattgagc ttcagcggtt cgcaaaacta tacgacaagg
     1501 atataaaata ttttgtgaag taacaaaaac atgaactgcc aaaaatgcgg gaataaaata
     1561 aaggagggcg atgagttttg ccagtcgtgc ggagtacctg tggtaaaagt cgcaaagggt
     1621 gttcgcaaac tgcttagagg aagagttgga agaaaatatt tttttcttgg attattctcg
     1681 ctaatgttag cgtttgcggt aattcttgga attgttttta tatgttttgc tctgctcgct
     1741 agtatcttgc ccgatggcat tgcgttagtg atcagatggt tcggtggcgc actcatctta
     1801 ctgtgctcac tcatcgcatt atcttttgta gtctccttgt atgtaaggag aatacatgac
     1861 atcggtaagt ccgcgtggtg gcttttattg ctacttgtcc cttttctaaa ttacgtcgtg
     1921 gtgttctacc tcctctttaa aaagggagag gcttcaagaa acagatttgg agacgagccg
     1981 ttaagcgagg ctggattgat ggacacgata tttaatcgtc aaccttaaca atttaattat
     2041 cccgcactaa aaaggtcgtc agatttatgg aaacaaaaac actaccagcg gttgcgtttt
     2101 ttttgaccct ttttctggct acgagtatag tgctggcggc tccaactgct ccatcaaaaa
     2161 gtaagactcc caatataaaa actctttctc caaacaccgg tggagaggga gaggtaataa
     2221 ctatggcggg aagtaacttt ggtaaagacc ctgctgatgg catcatcggg ctgaacgatg
     2281 ggccaaatta cgtgagagtc ggcggttttt taggaacgga catgatctcg tgggcaaaca
     2341 acaaagtgca aatattcgca ccctcgttac aagacgcggc gagcaatctt tcgagaatgg
     2401 ggatattttt taccgtcgcc gacagcatcg gtaaaaacat cccaggtttt aacctttttt
     2461 tgtacaccat gaaacttttt gatgccgtga caacgcagaa atcgacgttt gtcgataaca
     2521 cagcgcccgt aacagtcgtt gtgtatccat tgaattcaag caacacagaa tattttacct
     2581 ataaagccaa aacttactcg cctggcataa agggtttggc tgaaaaggtt atagacgcca
     2641 cagatgcagt acacgtggca aaaataacat acgattcttt cgggaaagat attgttggat
     2701 tagtgactaa cccagtacag ctcgtaaagg ataaattgcg agacaatgcc cgtagtgctt
     2761 ttgagacccc gttgcttatt caagacgctt tttccgggtt taagtcatca gaaataacac
     2821 cttcccctcc cgaggcgaat attgtggctc ctacccaaaa acaaccgtcc cctattggtg
     2881 aaggtcagaa acaaccatca aaagaaacca aaaaggatac gaccactacc aaggtaactg
     2941 aacagaagcc gccaaccgaa cagaaagcaa caagattgac gataaaatta gtggatcaat
     3001 acgatttacc aataactgag gtgaatgaga gcgagtattg tagaacgaaa ttgccctcag
     3061 aggtggaacg cgctggagcc tgccatgagt tagggactag tcccactctc cttttaatag
     3121 attccgttgg agataaggtg ccaggatttt ttgccagatt tgataagaat gaagatttct
     3181 tttatggcaa tagctcacca acttccccaa gaggagagtg gacatatgat agagatatag
     3241 taccaggaaa atatactctg gtgttttata agggaggaag tactgaaaat agcttcacta
     3301 gaagattcta taactcttct gaaaaacaag tcagcgtttc taatagtgaa gggtggtttg
     3361 taaaaatgac ggtatctcaa attattacac atgttttctt tgaaattcat ttaaaaatag
     3421 tcaatcaggc cggtacacct atggaccctg ggggttatta taatcaggca ggtgattatg
     3481 ttgcgggctt tgttatgaaa gacaaaaatg gagaagatgt gagaatagat gcaaaggcgg
     3541 tagagaatgg tcaatatgtt ataagaggtg tatctacttg gcttgggaac tggtcatatc
     3601 cgcccgggga ttacatcctt cgtgttgaga aggaggggta tgcgccagtt gaaaaaacta
     3661 ttcaaattcc ggatttgtca cgcatttccg acgacgaaat aaaattgaaa cgacaagaca
     3721 aaatagatct tgggactatt acacttacaa aaatatagta tacacaaaac ttgcaagaaa
     3781 tgcgacgcgt tcaattcgga acatgctgga acgtggacag tggcttcgcc aaagagcgga
     3841 tatgttccgg tgattattaa taataattat tagtattatc aactaatcca cttaactata
     3901 tgacaaaaaa tcagatcata ggtgtggttg cagtagttgc ggtcgttgtc ggtgcgtttt
     3961 ttttcggaac ctacagcagc aacaaggcga gcagagatgg tgaaacagat tacgatgtcg
     4021 ctttggacaa ggcggcgtca attgtttcgg cgtctttaga cgccgttcgt tccgaagcca
     4081 gcgctaaagc ccacgacgca cgccggatta ccgacctaaa gcagcttgag cttgcccttg
     4141 aactctatta tggttccaac gagagctacc ctctctccat tgggagggga actatcggca
     4201 gtttggcggg ggccggttat atatccaatg tcccaacaga tcccgtttct aacgttacct
     4261 acatctacgc gcctcttgcg aaggctgggg ccgctactgc ggcggcgata tgttcttcaa
     4321 cgcaaccatg cgtaagctac gttctactcg caacgctgga aaacagcaat acaattccta
     4381 atacttccat taagactaac ccactcggag gccagaattg tagaacgact ggagtgtatt
     4441 gcgtagttcc ataaattcaa ttatcaattt atcaacaacc aacgtaatca tatgcacaga
     4501 atttgtaaga attgcggttc gtccaactcg gagcatgccc gcttctgcac gaagtgcgga
     4561 ggcgcgcttc attctcacga gcacgcgcac gaacggacac cggcggaaca ggccgctgat
     4621 gtgagcgaaa aggcaaaagt ggaggccaat aaagttatag agaagacaaa acacatctgg
     4681 tctggcttct caaaagccga aaagataata gcaatcggag ctatcgccgg acttgcggcc
     4741 ttcgtcttgc cgtgggtatc ggcaggcggt caatccgtaa gtggattttc agccgcctcc
     4801 aatagcggct atgtctacct gttgccactt ctcatggttg cctctctctt gcttctctac
     4861 ttcacacagg gagcgtcaga cactcgcaaa gtgcttatga cgcgctggca aatcatcatc
     4921 gggtcagtca caagtaccgt cggcctcttt atgattatct tcatcagcac catcggcagc
     4981 ctgatgtcgc agatgatggg cggtatcggt gctttattcg gtggtggcag tttcggcgtt
     5041 agcgcaggaa tcggcgtata tctctacgcc gccggtgcta tcgccgttgt cgtgggggct
     5101 ttcagacttc agggcgaagt cttgcaacgc cttagagaca aagattgatt attaatttat
     5161 cagtaactaa tttctaaacg tatgtcagac ccaacaacat cattactcgg cggcatgttc
     5221 gccttctacg gttttacgat gttactgttt ctcgtcctcg gcattgttac gttcgtcttc
     5281 tggatcaata tgctcatcaa agcgataaag aacgaatacg agcacaagac gctctggatt
     5341 ctcgcaatca ttttcggcca agccgtcgga gctattctgt tctacttcat cgtctataaa
     5401 gagaaaatca aaccgcaggt ggctaaagtc gtttctcaat aagattataa gagattaaga
     5461 ataatatatg acaaatagta aattggtcgg agcgggcgta ctgatagtcg tcgggttagt
     5521 ggtaattacc gcaggaagca tggcgttgcc gttgctaagt cgcggcgact catatacgta
     5581 taaagttgtt gagcgttgtg attggaattt ccatccgttt gaagatcagt taaatcaaac
     5641 agccaaagat gggtgggagt tcatagcggg tatcggcgca aataattgcc atcttgtttt
     5701 taggaagaag tgagcggtag tttatttact ctccctgaaa ctttcttgtt ccgcgctctt
     5761 tataagctcc tcgcgccgcg ttttatattc tttctctacg ttctcgcgcg ggcggaggtg
     5821 tgagccgccg atacgaaagg tggcaacctc cgccctgaac tcctcctcgt ccatgtctgc
     5881 cgtttcgtaa ggcggagcta aattgagggt gctgatggcg ataacgccgc cttcaatctt
     5941 gttcttgacg acacaagctc ggtgaggcaa ctcttggagt tgccggatat accactccca
     6001 cccgggcggg tcgttgtaga tggacgagag agattctttg gcgaggacgt tggaatcgtc
     6061 gcgagaaatg cggaagtagg cctgtaagcc gcagttggaa agaatggagg cgcgaagcgg
     6121 cggaagaagc tgggcgagat tctgatgcgc cagaataagc gacagcttgt attttcgcgc
     6181 ctcggcgagc gtttgcgcga agctctcggt agcgaagttc tgaaactcgt ctatgtaaag
     6241 gtagaacggc acgcgcttag aagccggtat atcggtgcgg gagaaagccg ccatctgcaa
     6301 tttcgccaag aggagcgagc cgagcaaatc ggcgttgcct ttcaagtgtc ctctgtccag
     6361 tttcaccaaa agaacttttt ggttatccat cacgtcgcgg agattgaacg tgcttttggg
     6421 cgaaacgaac atctgccgca cgttgtcgtc cgagagaaac gcattaactt tgttgagcgt
     6481 ggattctatc cattcgttcc gcgtatgcgg agcgagcgtg ttgtagcgtt caaaatactg
     6541 gcggcagatg gggtgttcta ctttctccaa aacaaacttg cggaaattga agtcggttaa
     6601 aaaaagcgga agctcggcga gcgtgagatt gttttcaata agggcgatta gcgtattttt
     6661 gagcaagtcc tccatacgcg ctccccaaga atctttccag attttcttaa aaacctccac
     6721 cagctcgctc gcgatacccg ccggagttat gccctccgtc ttttcaagag gattaaagca
     6781 cgtcgtagaa gcggggtcgg tcgggtcaat caagacgacg cgttctctca aaaactccgg
     6841 ctcgtccttt ttgagcagat acagatagcc ctttaaatct tccgtcaggt cgccgtgcgc
     6901 gtctatgacg ccgaagccgt ggccgttttt aatgtcctgc tccgcaagcg tttctaaaaa
     6961 cttcgtcttg ccgctaccgg aagcaccgac gacataaaag tgcgttgagc ggtgttcctg
     7021 cgaaacgccc ttcggaagaa tagtcgtctg tttggttgtc tcgtccgtgc cttccgccca
     7081 gcccagcacc aattcatccg gcgttggttc tcgttggagt atctgcgtgg cctcctcttt
     7141 gttggaagcg ttcaacaaat tctcgcccca aaagagacgc tcaaagaagg cttttatttt
     7201 ctcgagtttg ccggacatga atgattaggc gatattaagg aatccttttg ttctcttttc
     7261 tattccggta aagtctagtt tttgattttc catcacaact ttgactgaat ctaaaagtgc
     7321 gtgatgaacg gttgtcgtat aggcggtcaa ttcttcggta tcaaagagat tgagcaggct
     7381 cataaactgg ccgcttccgc cctgcatctt ggcgatagcc tgtccgaata taagaagcgg
     7441 taatttgaaa atcgcaagga ttgggtttcg tttaaagagc cgccaaaagg aaattggctc
     7501 ctcaatgaga caccatgaga caaagagttg tttgccgtag tctcgcgctc cgatatacat
     7561 atcgtaaccg tcaagaattc ggtgctccgc gatgagaaac tcgcgcggcg ttttggtgaa
     7621 tccggggccg ggcttgcgct tctggcggga aacgaagatg ttaggcgctt caagcttttc
     7681 aataattcgt atggtttctg cgataacctg ttctccttgg ccgtttgccc catcaatgag
     7741 cgttgaccaa cggtcaatga tcttttcctg ttttatggtt ttggtaaatc cgaacattcc
     7801 gtcaacaatg ttcccgcctt tgagtttctc ttttttcaaa ccgccctcct gtttgttttt
     7861 ataagccaga taaacgatga ccccgattac gacgatgatg ataaacgtcc acatagagaa
     7921 agattattaa aagcgaataa tataaacgag ctactttcag acgattgcct gcgggtcgtc
     7981 gttcttcggc gaccgccacg ctggctttag caacgcctcc ggcgtagccg tattgtatcg
     8041 cgtttccgaa aggaagagat acatcaaact gccctcatca aactgccctc gcggcggtca
     8101 tccgcgtctt tcgcactgcg acagagattt gctgacctgc cctcgttcgg cgttatcgtt
     8161 agcacgcgga aattagaaac gccgagcgtg tctttgaagc tctcctcgcg gttgtgccgc
     8221 caatacattt tcaacttatt tacataccgc tcctgtgtca tcgtcgctcg gtcggcttca
     8281 agaaaacacg gatatttatg cgtcggcgtt tccaaaacga aaaaagcatc tggcactaac
     8341 gggggatttg cgccgcgctt cgcaagcgcg gattttaaat cgttgccctg cacccatcgc
     8401 gccagtttta tatcg
//
LOCUS       DATDSU010000094        10297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2422205, whole genome shotgun sequence.
ACCESSION   DATDSU010000094 DATDSU010000000
VERSION     DATDSU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2422205"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1339
                     /gene="serS"
                     /locus_tag="VI981_03165"
     CDS             83..1339
                     /gene="serS"
                     /locus_tag="VI981_03165"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY4479329.1"
                     /translation="MLDIKFIRENLEIVKAGAKKKRIEIDLDKLISIDDGRRELMTHV
                     ETKRAEQNSYNQKIAGASAEARAPLLAQMKELKDALQKEEEKLKEIMKEWHALMLSVP
                     NIPDISVPEGKDEADNVEVQRWGTVPSFSFPVKDHIEIMEKLRMVDFERGTKAHGFRG
                     YFLTGQGADLSWALWNYARDFYGKRNFVPFITPTIVKKEFFYGTGHLPREAEDLFKTQ
                     DDDYLSGTAEVPMMAYHSDEILKESDLPKRYLAFSPCYRREAGSYSKDTKGILRVHEF
                     FKVEQLIISKADHVESAKLHEEINRNFEEFIESLNLPYRRLLICGGDLSASKVKQYDT
                     EAWFPSQKAYRELSSASYYHDFQTRRFNTRYDEAGKKRYAHSLNCTAVATPRIIAALV
                     ENNQQADGAVKIPQVLEKYMGFDIIR"
     gene            1463..2278
                     /locus_tag="VI981_03170"
     CDS             1463..2278
                     /locus_tag="VI981_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479330.1"
                     /translation="MRVFRSTRNHRSRHQFFLECSLTLVFALCFLGALAGLSRLEFLT
                     IDSIDVAGTKALSKDAVIKSVERELEGSKLLLFPKRNIFIADTDALSRTLQDEIKVID
                     EVRVSFRDVHRLVVNISERTPHYLWCGENVSDGESCFFLDQGGFIFSPAPKFTGIVYL
                     RVYGALEGQGTIGNGFLDEVTFRKIGSFVTAMKQAGFNSSKLIAKGDAEFELYLENGT
                     KILFNSSQNLDRALDNLLLVFSPSESVKKKTTAGLLYIDLRFNNKVFFKEKES"
     gene            2296..3192
                     /locus_tag="VI981_03175"
     CDS             2296..3192
                     /locus_tag="VI981_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479331.1"
                     /translation="MLAKTIVLNNRVFTYVKSRTYMPVSVYKGKNVFLRVGPEELIKK
                     EIKSSKELFKLGYPVAEILESGHLDGQYYFIENSLGEIQFSSIFESDTEKEGKINDEH
                     FALFLDVSIRLAEAQIKTASKKELWDEFMGGIHLGHIINELPQLASQTKIATEKVRNR
                     LKAMPRVMSHGDFNPYNSFPGGIIDIERPFAAPLGYDLASNIIQTFMFPSKNNYEKHR
                     KYEFNPSQIDRYVLEMDRLLHKEDLLELSKILDDFIFLRLIWSVVRMEKTPLLQKWRY
                     GLYTRVLMAYISNFPIKPLVFR"
     gene            3236..3622
                     /locus_tag="VI981_03180"
     CDS             3236..3622
                     /locus_tag="VI981_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479332.1"
                     /translation="MHIALIGGSYMYWHYVLAWKSIWAIWKNIAWFLTHFFSLRLLTR
                     TFFTPWKRMQEERKRGFSAADFFSSILVNTILRLVGMAARAAVLLVGLVALIVHAVIG
                     IVVFAAWLFVPFVLLAGFIKGVLMIF"
     gene            4000..6414
                     /locus_tag="VI981_03185"
     CDS             4000..6414
                     /locus_tag="VI981_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4479333.1"
                     /translation="MKFIDIKKEAKIFRPAILLDKIISRTHRYFIRNMSGYCAVLFAG
                     YFVFVKEDERIGGLFFITISIWALFLLLDFFYNSNYVGSAIALHRQNISFAAAALLYE
                     TLDDDITRGLLFSRLGRILRIRLGIDQASFAAFFETRKTPLRSEAFEVSDNTLTVALY
                     AKAVLDNDKEFKDMLIKLGITESIFLGAVSWIEEAEHLRLEQFRWWTRDRLSQKSQIG
                     KNWSYGGAFFLERFAKDIKSMSNTLGFLDVPGYERKEGKEIEAVLSRRREANALIISE
                     KGEGGLETVFHLWKRIHNGTVPPELCLKRVMVLDTNMVVAFAKDKATFEQTLIKILND
                     AVKAGNIILVIEDLPSFVLSAKGLNSDIADLVEQYLASPSLQVIALSDRGRYHEFVEP
                     IKIFGERFEIIKIKAEDDNALLAILKHEALVVENRDKIFFTYQALDTIVKDAERYFSE
                     GVTLDKVIDLLEEIVPKAKQEGVVLITRTFVENLVEQKTGIPLGDVSETERQKLLTIE
                     DDMRGRIVGQEIALRAIGSALKRSRGGVANTKRPIGSFLFLGPTGVGKTETTKAIADL
                     YFGNEQSLIRFDMTEFTGQDGLAKLIGSFEGSKVGLLVKALRDRPYGVLLLDEFEKAS
                     TEVRDLFLQVVDEGFFTDSHGNRVNALNLIIVATTNAGSSLIWEIMRKGDDLLKAKDT
                     IIDHIIKEGIFRPELLNRFDEVVLFHPLDKEQLAKIAELMLGKLKRRLEDKGVVFEGD
                     ADLVPYLVEIGSTEQFGARAMNRAIQDSVEKFIAEEILEKNLRTGATVHVSVSNLTKI
                     PIQHTI"
     gene            6669..7727
                     /locus_tag="VI981_03190"
     CDS             6669..7727
                     /locus_tag="VI981_03190"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY4479334.1"
                     /translation="MRKLARLEDERVSKYVTLPTPRELKQQYPITAAGKRTVAAARQA
                     IIDMMTGKDRRMLAIVGPCSLHNEHEALKYAHRLRECAETVQDDVVILMRAYFEKPRT
                     TIGWKGMIYEPDLNGESNIQKGVRLTRKVASSIVNLGVPIATELLSPNLFQLCLADLV
                     SYASLGARTSSSQIHWEISSGLTCPVGVKNPVTGDVTPAIDALESIRHVHSFASITQQ
                     GKFAEVFTKGNKWSHIILRGGKVPNYDSDAVGAAQDQLRDRKLSPFIIVDCSHGNSGK
                     IAANQKRVLESVIKQRLLGNNDLGGVMIESNLASGKQPVQPLENLLPGISVTDDCLGI
                     DDTISLLRAMRLALQKAR"
     gene            7843..>10297
                     /locus_tag="VI981_03195"
     CDS             7843..>10297
                     /locus_tag="VI981_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479335.1"
                     /translation="MHSRLPLITVSFAIVVLIGTFIFDQYINSLQSIPTEEGQSKEQK
                     LVAAVKQASGKKDNSEIYNTVSESADLIARLAGDLSVLVEDSKKTSLNSDSLKKVKLA
                     RAIASERKKKALDLMKSDPLLFSKKAVKKDVWKNLPPEIQKDVEEEVTLTGTLQVIHV
                     DDFEDGGKSDFIYKLKSNGKEYNLYLSKKRPLMSGTEIRVQGFLLDNILADAGGAGEI
                     QIMADAPAVEAVGNQRILVLLLTPSSTLTLPFTRSQAEGFAFSGQFANFMREQSYNKV
                     SFSGDVYDWIVYPDASSCTILDAPTLGNIASSKGIDLSLYDRIVYFINGSYGGCSTIG
                     KSDYFINGFHYRFSQASVSALGSQINTVMPFSWTSLDSTLSHEIGHSLGVMHANGWDC
                     DSSILYGYCNHVEYGNPFDVMGTRYVSLNFNALYKESLGWLEPSRILTITNSGTYTIN
                     NLESPTGIASVKIKTLDGLSTPFIVERRRPIGFDAGIGANDYKNNSNGLLINKIVDDY
                     FPFPRLLDMSPVAGAWRIDALNAALTGSQVFHDEGTGISVGPIRSVSDTAVSFDVSVA
                     TPTCVRLVPQQINATPFSTRSVRGSLYPITYSVNALNGDIGCGDSRFRIGVISPVGWG
                     MSPFFETELVSTGERALGSASLLIPSTISLGTYLVVIEVSNNQSGLSTTISVQLEVVD
                     PPRLDSLNPASGPGGTSVILSGKNFYADSTGNVTVNFRGASGFGSIVGSFTNNTTATF
                     NIPTTVTVCDADCREVATPDGLYDISIVGVGGTVSNTLSFEVKPLPLTVIYPNGGEKW
                     QVGETYTIQYDLPTPSHKAL"
BASE COUNT         2879 a         2116 c         2460 g         2842 t
ORIGIN      
        1 atccgactca ttgtcggttt ttttattttt ggaatttccg ctatactaaa ctctacacgc
       61 taaaacctaa acccttctgc ctatgcttga tatcaaattt attcgggaaa acttagaaat
      121 cgtaaaagcc ggcgctaaaa aaaagcgcat tgaaattgat ttggacaaat tgataagtat
      181 tgatgacggg agacgtgagc tcatgacgca tgttgaaacg aagcgtgcgg agcagaattc
      241 ttataaccaa aaaattgcag gagcgtctgc tgaggcgcgg gctccgcttc ttgctcaaat
      301 gaaagagcta aaggatgcct tgcaaaagga agaagaaaaa ctcaaagaaa ttatgaagga
      361 atggcatgca ctcatgcttt ccgtcccaaa cattccggac atatcagttc cagaagggaa
      421 ggatgaggcg gacaatgttg aagttcagag gtggggtacg gttccctcgt tctcgtttcc
      481 cgtgaaggat cacattgaaa ttatggagaa acttcggatg gttgattttg aaagaggtac
      541 gaaggctcat ggtttccgcg gctatttttt gacgggacaa ggtgcggacc tctcgtgggc
      601 gctttggaac tatgctcggg atttttatgg aaaaagaaat tttgtgcctt tcataactcc
      661 cacgatcgtc aaaaaagaat ttttctacgg cacgggacat ttgccgagag aagcagagga
      721 tctttttaaa acgcaggacg acgattatct gtcgggaacg gccgaggtgc cgatgatggc
      781 gtatcactcg gacgaaatat tgaaagaaag cgatctgccg aagcggtatc ttgcattttc
      841 tccttgttat cggagagagg cgggaagtta cagcaaggac acgaagggca tactgcgtgt
      901 gcatgaattt ttcaaagttg agcagcttat aatttcaaaa gccgatcatg tagaatctgc
      961 gaaactgcat gaagaaatta atcgaaactt cgaagagttt atcgagtcgc taaatttgcc
     1021 ataccgtcgt cttttaattt gtggaggtga tctctcggcg agtaaagtaa agcagtatga
     1081 cacggaagca tggttcccgt cacaaaaggc ataccgtgag ctctcaagcg catcgtatta
     1141 ccacgacttt caaactcgtc ggttcaatac tcgatacgat gaggcgggaa aaaaaagata
     1201 tgcccattct ttaaattgta ctgccgtagc aacgccgcgc attattgcgg ctcttgtgga
     1261 gaataatcaa caggcagacg gcgctgtcaa aattccccaa gtgctcgaga aatatatggg
     1321 atttgatatt atacgatgag tcccgttagt ggccgcggtc acgggggaac aaagtgatca
     1381 gtctgatgaa aaactttgat tttacatttt attcattaaa aagcaggggg aaaatgaccg
     1441 accccgatct gccactaacg ggatgagggt atttcgaagc actagaaatc accgctcgcg
     1501 tcatcagttc tttttggaat gttcgctcac actcgtgttt gcgctctgtt ttctaggggc
     1561 gctcgcgggc ctctcaagac tcgaatttct tacgatcgat tcgatagatg ttgccggtac
     1621 gaaggctctc tcaaaggacg ccgttatcaa aagtgtcgaa cgcgagcttg aaggctcgaa
     1681 gctcctactg tttcctaaac gcaatatttt tatcgccgac acggacgcac ttagccgaac
     1741 gcttcaagat gaaataaagg ttattgatga agttcgggta agtttcagag atgtccaccg
     1801 gctggtcgta aatatttcgg agcggacccc gcactacctt tggtgtggag agaatgtttc
     1861 ggatggcgaa tcgtgtttct ttctcgatca aggcggattt atcttttctc ctgctccaaa
     1921 atttaccggc attgtatatt tgcgagtgta cggcgcactg gagggacagg gaaccatagg
     1981 aaatgggttt cttgacgaag ttacgtttcg aaagataggg tcgttcgtga cggcaatgaa
     2041 acaggcagga tttaattcgt cgaagcttat tgccaaaggc gatgcggaat ttgagcttta
     2101 tcttgaaaat ggcaccaaaa tacttttcaa ctcttcccaa aatctggatc gcgcgctcga
     2161 caatttactg ctcgtatttt caccaagcga gtctgtgaaa aaaaagacga ccgccgggct
     2221 tttgtacata gacttgcgtt tcaacaataa agtttttttc aaggagaagg agagttagaa
     2281 gactaatacg acctcatgct ggcgaaaaca attgtattaa ataaccgagt ttttacatat
     2341 gttaagtcaa ggacatatat gccggtttct gtatataaag ggaagaatgt gtttctgcgt
     2401 gtcggaccgg aagaattaat aaaaaaagaa ataaaatcga gtaaagaatt attcaagttg
     2461 ggttacccag tcgctgaaat tttagaatca ggacatcttg atgggcaata ttattttatt
     2521 gaaaattcgc tgggtgagat tcagttctct tcgatttttg agagtgatac agagaaagaa
     2581 ggcaaaatca atgatgagca ctttgcttta tttcttgacg tatcgattcg tcttgcggaa
     2641 gcccagataa aaaccgcatc aaagaaagaa ctatgggatg agtttatggg aggaattcac
     2701 cttggccata tcattaatga gctgcctcaa cttgcatctc aaactaaaat cgcgacagaa
     2761 aaagtccgaa accgattaaa agctatgccc agagtgatgt ctcacggtga ttttaatccg
     2821 tataacagtt ttcctggagg cataattgat atagaaagac catttgcggc ccctcttggg
     2881 tacgatctgg cttcaaatat tattcagacg ttcatgtttc cgagtaagaa taattatgaa
     2941 aaacacagaa aatatgaatt taatccgtcc caaattgatc gctatgtcct agaaatggat
     3001 cgcttgctcc ataaggaaga cttgctcgaa ttgagcaaaa tcttggacga ttttattttt
     3061 cttcgcctta tttggtctgt tgtaagaatg gaaaaaactc cattgttgca aaaatggcgt
     3121 tatggtctat ataccagagt gcttatggcg tatataagca atttcccaat taaaccactc
     3181 gtcttccgat aaatttccac agacgaatat tctcgggcat cgtataatac tgtctatgca
     3241 cattgcgctt attggcggct catatatgta ttggcattat gtcttggcgt ggaaaagcat
     3301 atgggccata tggaaaaata tcgcatggtt tttgactcac tttttttcac ttcgacttct
     3361 gactcgcact ttcttcactc cgtggaaacg aatgcaggaa gagcgaaaaa ggggattttc
     3421 ggcagccgat tttttctcat cgattcttgt taacacgatt ctaagactcg ttggtatggc
     3481 ggcgcgcgcg gctgtgctcc tcgttggact tgttgcgctc attgttcacg cagtaatcgg
     3541 catcgtagtt tttgcagcat ggctttttgt gccttttgtt ctgcttgctg gatttataaa
     3601 aggtgtactc atgattttct aggatgaatc ccgttagaaa tccaagtgtt atgtatttac
     3661 tacctatctc aataaaatca agtaatcgag gtgctgagac gcttataata catgtgacgg
     3721 gtgcgccttc acgaaaaacg tctgacgtta ccaacggtgc acatgcatta ctagctaaga
     3781 gcgctcttga gacacgtatg ctgggcgcac cctatttcta acgggatgaa tcccgttaat
     3841 cccgattaga agtaagaact tctaacggga tttaagaatt cgagattaag ggacttgtcc
     3901 cgttaaaaaa taaactttgc atgacttcca aaatatttaa caagcaaaag acaaaggacg
     3961 agcgtattac agtttcaaga attttaattt tttaacggga tgaaatttat agatataaaa
     4021 aaagaggcca agatttttcg tccggcgatt ctgcttgata aaataatttc acgcacgcac
     4081 agatatttca ttagaaatat gagtgggtac tgtgcggttc tctttgcggg atattttgtc
     4141 tttgtgaagg aggacgagag aatcggcggt ttatttttta ttacgatttc tatctgggcg
     4201 ctatttctac ttctagattt tttctacaat agcaactatg ttgggagcgc catagcgctc
     4261 cataggcaga acatttcctt tgccgcggcg gctcttttgt atgagacgct ggatgatgat
     4321 attacgcgag gcctcttgtt ctctcgcttg gggcgaattt tgcgtatacg tctcggcatc
     4381 gatcaagcgt ctttcgcagc tttttttgaa accagaaaaa caccgcttcg aagcgaggct
     4441 tttgaagtgt cggacaacac tttgacagtg gctctctacg cgaaagccgt cctcgacaat
     4501 gacaaagagt ttaaagatat gctaatcaaa ctcgggatca ctgaaagcat ttttcttggt
     4561 gccgtttctt ggatagagga agccgaacat ctgcggcttg aacaatttcg ctggtggacg
     4621 cgggatcgct tgtcacaaaa gagccagatc ggaaaaaatt ggtcatacgg aggagcgttc
     4681 tttcttgagc ggtttgcaaa agacataaaa agcatgtcga atacgcttgg gtttctcgat
     4741 gttccggggt acgaacgtaa ggaaggaaag gaaatagaag ctgtgctctc gcgccgcaga
     4801 gaagccaatg cgctcattat ctccgagaag ggcgaaggtg gcctcgagac cgtttttcat
     4861 ttgtggaagc gtattcacaa tggcactgtg cctccggagc tctgcttaaa gcgcgtcatg
     4921 gtcctcgata ccaatatggt cgtggccttt gccaaagata aagccacgtt tgaacaaacg
     4981 ctcatcaaaa ttctaaatga tgccgtaaaa gcagggaaca taattctcgt cattgaggac
     5041 ttgccttcat ttgttctttc tgccaaaggg ctgaactctg atattgctga tctcgtagaa
     5101 caatatcttg cgagcccgtc acttcaagtg atcgcgctct ctgatagggg acgatatcac
     5161 gagtttgtgg aacctattaa gatattcggt gaacgttttg aaatcataaa aattaaggca
     5221 gaagatgaca atgcgcttct cgcgattttg aaacacgaag cactcgtggt ggaaaatcga
     5281 gataaaatat tttttactta tcaagcgctc gatactattg tcaaagatgc agagcgctat
     5341 ttcagtgaag gcgtaacact cgacaaggtg attgatcttt tggaagaaat agttcccaaa
     5401 gcaaagcagg agggcgtcgt gctcattacc cgtacctttg ttgaaaatct ggttgaacaa
     5461 aagaccggaa ttcctttggg agacgtttct gaaactgaac ggcagaaatt gctcacaata
     5521 gaagacgata tgcgcggacg tattgtcggg caagaaattg cacttagggc tatagggagc
     5581 gcgctcaagc gttctcgcgg aggtgtggca aacacaaagc gcccaatcgg ctcgtttctc
     5641 tttttggggc cgacaggcgt tggcaaaact gaaaccacaa aagcgattgc agacctctac
     5701 tttggcaatg agcagtcgct tatcaggttt gacatgacgg aatttacggg acaggatggt
     5761 ttggcaaaac ttatcggttc atttgaaggc tcgaaagttg ggcttcttgt aaaagcgctc
     5821 cgagacaggc cgtacggcgt attacttctc gatgaatttg aaaaagcaag cactgaagtt
     5881 cgagatcttt tcctgcaagt agtcgatgaa ggatttttca ccgatagtca cgggaacagg
     5941 gtcaatgccc taaacctcat tattgtggcg acaacgaatg ctggcagttc gcttatttgg
     6001 gaaattatgc gaaaagggga tgatttgctg aaggctaaag acacgatcat tgatcatatt
     6061 attaaggaag gaatattcag accggagctt ttgaaccgtt ttgacgaagt cgtgctgttc
     6121 catccgctcg ataaagaaca gcttgcgaag attgcagaat tgatgctcgg taagcttaaa
     6181 aggcgccttg aggacaaagg cgttgtgttt gaaggtgatg ccgatcttgt gccgtatctt
     6241 gttgaaatcg gaagcacaga acagtttgga gcgcgcgcaa tgaacagagc gattcaagat
     6301 tcggttgaga aatttatcgc cgaagaaatt ttggaaaaaa accttagaac cggtgccact
     6361 gtgcacgtta gcgtttcgaa tttgacaaaa atacctattc agcatactat atagtcatga
     6421 attttaggta tggctttagc ttcttctatt tttggagctc tctcgagagt ccatctttgc
     6481 tgtgttaaag gccaaaacct tctagaacat caaaaacgga ctctcgagag ggtccgtttt
     6541 tgtttatcct gagcttgtcg aagagtttgc ccatcgatta agtcggataa acaaaatttg
     6601 ttcttttaaa atccgagaca ctggtgtctt tgatgcgatt ctttacaacc ctgaactcca
     6661 accgtacaat gagaaaatta gcaagactag aagacgagcg tgtttccaaa tacgtgacgt
     6721 taccaacgcc tcgagaactc aaacaacagt atccgattac tgccgccgga aagcggacgg
     6781 ttgctgctgc acggcaggcc attatcgaca tgatgacggg caaggaccgg agaatgctcg
     6841 ccattgtcgg accctgctca cttcacaacg aacacgaggc tttgaagtac gcacatcgcc
     6901 tgcgtgagtg tgccgaaaca gttcaagatg acgttgtgat tttgatgcgc gcctacttcg
     6961 aaaaaccgcg cacgacaatc ggttggaagg gtatgattta cgagcctgac ctaaacggcg
     7021 agagcaacat tcagaaaggc gttcggctaa ctcgaaaagt ggcctcgagc atcgtcaatc
     7081 tgggtgttcc tattgccacg gaacttctca gccccaactt gtttcagttg tgtttggcgg
     7141 acttagtgag ctatgcgtca cttggcgcgc gtacaagctc ctcccaaatt cattgggaaa
     7201 tctcaagcgg gctcacctgt cccgtgggtg taaaaaaccc cgtaacaggt gacgtcacgc
     7261 ctgcgattga tgcgctcgag tcaatcaggc acgtgcacag tttcgcgagc atcacacagc
     7321 aaggcaagtt tgccgaagtg ttcacaaaag gaaacaaatg gtcgcacatc attcttcgcg
     7381 gtggcaaagt gccgaactat gattctgacg cggttggagc tgcgcaggat caattgcgcg
     7441 accgaaaatt gagtcctttt atcattgtcg actgctcgca cggaaactcc ggcaaaatcg
     7501 cggcaaatca gaaacgagtt ctggagtccg ttatcaagca acgactactg ggaaacaacg
     7561 accttggagg tgtgatgatc gagagcaatc tggcgagcgg taaacaacct gtacagccgc
     7621 ttgaaaattt actgccgggc atctcggtca ctgacgactg tctcggaatt gacgacacca
     7681 taagccttct tcgggctatg aggttggctc ttcaaaaagc tagataatat cccacacgaa
     7741 ttttcggtgg gattttttat gaaagtctgt tctccaattt tagaaaacta gtgatcagtg
     7801 tggatgcgct cgagtgtatg cggatgtaca attgtcccat ctatgcattc acgtcttccg
     7861 ctcattacgg tttcatttgc tatcgtcgtt ttgatcggca ctttcatatt tgatcagtat
     7921 ataaactcgt tacagtccat accgactgaa gagggtcaat cgaaagaaca aaaattggtg
     7981 gctgcggtta aacaggcatc tggaaaaaag gacaattcgg agatttataa tactgtatcc
     8041 gaatcggcag atttaatagc gcgtttagcg ggtgatcttt cggtgcttgt tgaggactca
     8101 aaaaaaactt cactaaactc tgacagtctt aaaaaagtta aactggcgag agccattgcg
     8161 tcagagagga aaaaaaaggc actggacctt atgaaatctg atccacttct tttttcaaaa
     8221 aaagccgtga aaaaagatgt ttggaaaaac ttgcctcctg aaatccagaa agatgttgaa
     8281 gaggaggtca ctttaactgg tactttgcaa gtcatccacg tggatgattt cgaggatggg
     8341 ggtaagtctg atttcattta taaactgaaa tcaaatggaa aagaatataa tttatattta
     8401 tcaaaaaagc gaccccttat gtcaggtacc gagatacgag ttcaggggtt tcttttagac
     8461 aatatcttag ctgacgcggg aggggcaggc gaaattcaaa ttatggctga tgcgccggca
     8521 gttgaagctg ttggcaacca gcgaatactc gtgttactgc tcacgccgtc gtcgactctg
     8581 actctgccgt ttacgagatc gcaggctgaa ggctttgcat tcagtgggca gtttgcgaac
     8641 ttcatgaggg aacaatcata taataaagtc tcattttccg gagatgtcta tgattggatt
     8701 gtgtatcctg atgctagctc gtgtaccatc ttggacgctc cgactttagg aaatattgcc
     8761 tcatcaaagg gcattgattt gtcattgtat gaccggattg tttattttat aaatggatcg
     8821 tatggaggtt gctcgactat cggtaaaagc gactacttta taaatggttt ccactatcgt
     8881 ttttctcaag cgtcggtcag cgcattagga tctcaaatta acaccgtcat gcctttcagt
     8941 tggacgtctt tagactccac gcttagccat gaaatcggac atagccttgg agtcatgcac
     9001 gcaaatggct gggactgcga ctcctcgata ctctatggat attgtaatca cgttgaatat
     9061 ggcaaccctt tcgatgttat gggtacccga tatgtgtctt tgaattttaa tgcgctgtat
     9121 aaggaaagtt taggatggct ggaaccgtca cgaattttaa cgattacgaa ttctggaacg
     9181 tatacaatca ataatcttga gagccctacg ggaattgcct ccgttaaaat aaaaactttg
     9241 gatgggctaa gtactccttt tattgttgag cggcgcagac caatcgggtt cgatgcgggt
     9301 atcggggcaa atgattacaa aaataattca aacggtctct tgatcaacaa aattgtggac
     9361 gactatttcc cgtttcctcg cttgctcgat atgagtccgg tggcaggcgc atggagaata
     9421 gatgctctta acgcggcact caccggttct caagtatttc acgatgaagg aacaggtatt
     9481 tccgtaggac ctatacgcag tgtttctgac acggcagttt cttttgatgt gtctgttgcc
     9541 acccccacgt gcgtgagact cgttccgcaa caaataaatg caacaccctt ctctacgcgg
     9601 agtgttcgtg gttcgctgta tcctattact tattctgtca atgcattaaa tggcgatatt
     9661 ggttgcggag actctagatt tagaatcggg gtgatttccc ctgtcggatg gggcatgagt
     9721 ccctttttcg aaaccgaatt agtgagtacg ggtgagaggg cgttaggttc agccagcctc
     9781 ctcatccctt ctactatttc attaggaaca tacctagtcg ttattgaggt ttctaataac
     9841 cagagcggtt taagcacaac aatctccgtc cagctcgaag ttgtcgatcc accaagactt
     9901 gacagtttga atcccgcgtc aggtccggga ggcacatccg ttatattaag tgggaagaat
     9961 ttttatgccg atagtactgg gaatgtaact gtaaatttta gaggtgcaag tggctttggc
    10021 tcgatagttg gatcgttcac gaataacact actgccactt tcaacattcc gacgaccgtt
    10081 acggtatgtg acgctgattg cagagaagta gccacaccgg atggtttgta cgacatttcc
    10141 attgtcggag tggggggtac agtaagtaat actttgtctt ttgaggtcaa gcctctgccg
    10201 ctcacagtga tctaccccaa cggaggcgaa aaatggcagg tcggagagac gtacactata
    10261 caatatgacc tgcccacacc aagccacaaa gcactca
//
LOCUS       DATDSU010000095         3143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1274371, whole genome shotgun sequence.
ACCESSION   DATDSU010000095 DATDSU010000000
VERSION     DATDSU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274371"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..993
                     /locus_tag="VI981_03200"
     CDS             184..993
                     /locus_tag="VI981_03200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY4479336.1"
                     /translation="MPIQKPYYEKPKFKLYHADSLKLLQEMPENSVDMVFADPPYLLS
                     NGGFTVHAGRRVSVDKGAWDKSNGLKKDFEFHLEWIQAVWRILKPHGTIWISGTYHSI
                     YQCGFALQVAKFHILNDIAWFKPNASPNLSCRYFTASHETLIWARKDKNAKHIFNYDL
                     MKNGNWPEDQLKKPGLQMRSVWAMGTPKPIEKKFGKHPTQKPEDLLKRIVLASTNKGD
                     LVLDPFTGSSTTGIAAHLSGRHFIGIDNDRKFLDLSIKRFEDLERNLRGKK"
     gene            1000..1620
                     /locus_tag="VI981_03205"
     CDS             1000..1620
                     /locus_tag="VI981_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutH/Sau3AI family endonuclease"
                     /protein_id="HEY4479337.1"
                     /translation="MKIITRAVAIKNLKKHIGKDLRKLALEHGITTYETGKQNKGWKG
                     LVLERLAGLQNNVSKAPNGLTYELKSVSFHEIGNKLVPKETMAITMINPAELKAHDFF
                     ESHCWAKLKTIVFCAVMWHGQNSEKAELLKVTSLDFAEDDELIKEIKADYDFIRNKLK
                     TKGFAALTGADGKWIQARTKGPGHGSISRAFYARTALVKKIFEIAS"
     gene            1870..2958
                     /locus_tag="VI981_03210"
     CDS             1870..2958
                     /locus_tag="VI981_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479338.1"
                     /translation="MFKQIMNWDLVITWGKDILIVIGGMTVFFGFIKGLSDVIPQLGK
                     FKSWFWLQTAERLKNRTLVKNAIASDIETHVNEVVADLQRELPLGWIRKASIEWVTES
                     KENDIEEGEIILRVKPLENQDQNFLAGVYTFFSKALFPRTKEVIPTNVRKAAVLRISH
                     RTISSKKPYLRESFENSFLETAIREDPTIATYLGRYENLDKKGFFTGSFLREIHEIAT
                     KTRFTDLRNRMNQEVDEILTHIEGFISQLGVYPARTIPQGGWYRIGPATSYAFMLVAR
                     PEHGGVGPYVNRAKERLAQNIERLYVMGAKAEEPFARYVISEIAKLPNYKLVEVFELN
                     KDYRGNTGGIGALFVISQTGVSEEASEA"
BASE COUNT          975 a          638 c          766 g          764 t
ORIGIN      
        1 ccctcatatt cccaagtcct acttcactcc gctttgtgcc tccgttcgag cctgtggtat
       61 gaccccactt ttctggtcgc agagtccctg cctagaccgc ccttcgggcg gttgcagtcg
      121 ggcggcaatt ccttcccccc aattgagttg agttctcgat gaaattgaga gagaatacat
      181 taaatgccga tacaaaaacc atattacgaa aagcccaagt ttaagctcta ccacgcggac
      241 agcttgaagc tcctgcaaga aatgccggag aactcggtag atatggtttt tgccgatcct
      301 ccgtatcttc tttcaaacgg cggttttact gtccatgccg gacgacgcgt tagcgtagat
      361 aaaggagcgt gggataagag caacggacta aaaaaggatt ttgagtttca cttggagtgg
      421 atacaggccg tatggcgtat actgaagccg cacggcacaa tttggattag cggaacgtat
      481 cactccattt atcagtgcgg cttcgcgctc caagtcgcca aatttcatat cttgaacgat
      541 atagcatggt tcaaaccaaa cgcgtccccc aacctaagtt gccgctattt cacggcgagc
      601 cacgagactt taatatgggc gcgaaaggac aaaaatgcca agcatatttt taactacgac
      661 cttatgaaaa acggcaactg gccggaagac cagttaaaaa agccgggcct tcaaatgcgt
      721 tcggtttggg ctatgggaac gcccaaaccc atagagaaaa aattcggcaa gcacccgaca
      781 caaaagccgg aggatttgtt aaaaagaatc gtgcttgcga gtacgaataa gggtgacttg
      841 gttttagatc cgttcaccgg aagctcgacg acaggcatag ccgcccatct ttcggggcgg
      901 cacttcatcg gcattgataa cgaccgcaag tttctggact tatccataaa gcggtttgag
      961 gatttagaaa gaaatttgag aggtaagaaa taaaacaata tgaaaatcat cactcgcgct
     1021 gtcgcgatta aaaacctcaa aaaacacatc ggtaaagact tacggaaatt ggccttagag
     1081 cacggcataa caacttacga aaccggcaag caaaacaagg gctggaaggg actcgttttg
     1141 gaacgactgg ccggattgca aaacaacgta tcgaaagctc cgaatggcct tacctacgaa
     1201 ttgaagtcgg tgagcttcca cgaaataggc aataaactcg tgccgaaaga aacgatggcg
     1261 ataacaatga taaatccagc agagctaaaa gctcacgatt tttttgagag ccattgttgg
     1321 gcgaaactca aaaccatcgt tttttgcgcc gtaatgtggc acggacaaaa ttcggagaaa
     1381 gcggaattgc tgaaagtgac gagcttggat tttgctgagg acgacgagct tataaaggag
     1441 ataaaggctg actacgattt tatccgcaac aaactcaaga ccaaaggttt cgccgcattg
     1501 accggagcgg acggaaaatg gatacaggcc cgaaccaaag ggccgggcca cggatcgata
     1561 agccgtgcgt tttacgcacg aacggcgtta gtgaaaaaga tttttgaaat tgcttcataa
     1621 atatatgaga ctcttttatt tcaaaattaa gaatttcttc aagacattac ctgaactgtt
     1681 taagagtacc aaagtgaaaa tggtttatga ctccgatttg agcaatcttg tcgaatcact
     1741 gggtataaaa acgaaaattg aaaacggcga ttttctttgt agattttgca atgttcaaat
     1801 cactcttgaa aatttggggt caatacaaaa agatgttggc gagttgagat ttacctgtac
     1861 aaatcttgaa tgtttaagca aattatgaac tgggacttag ttattacttg gggaaaagac
     1921 atcctgatcg ttatcggggg gatgactgta ttctttggct ttataaaagg attgtccgat
     1981 gttatcccgc aactagggaa gttcaaatct tggttttggc tacaaactgc agagagactg
     2041 aaaaatagaa ctctcgtaaa aaatgcaatt gcaagcgata tagaaactca cgttaatgag
     2101 gtggtggccg atctccagag ggaacttcca ttaggttgga ttagaaaggc ttcgattgag
     2161 tgggtcacgg aatctaaaga gaatgacatc gaagagggag aaatcatctt aagagttaag
     2221 ccgcttgaga atcaagatca gaactttcta gctggtgttt acacattttt ttcaaaagcg
     2281 ctctttccac gcacaaaaga agttatacct accaatgtaa gaaaagcggc tgtactaaga
     2341 atctcccacc gcactatttc gagcaagaaa ccatatcttc gagaatcatt tgaaaactcg
     2401 tttttggaaa ctgcaatcag agaagatccg actatcgcaa cttatttagg ccgatatgaa
     2461 aatcttgata agaaaggatt ctttaccgga agctttttgc gtgaaataca tgagattgca
     2521 acaaaaacaa gatttactga tttaagaaac agaatgaatc aagaagttga tgagatacta
     2581 actcacattg aagggtttat atctcaacta ggagtttatc ctgcgagaac gataccgcag
     2641 ggagggtggt accgtattgg ccccgcaact agttatgctt ttatgttagt cgcccgtcca
     2701 gaacatgggg gtgtcggacc ttatgtaaat cgtgccaaag aaaggcttgc ccagaatatt
     2761 gaacgacttt acgttatggg agcaaaagcg gaagagccat ttgcaagata cgtgatatca
     2821 gaaattgcga agttaccaaa ttataaactt gtagaggttt ttgagctgaa taaggattat
     2881 cgagggaata caggaggtat cggagcctta tttgttatct cccaaacagg ggtatcagag
     2941 gaagcttcag aggcttgaga gagtatgggc tttcctcccc tgtcgggcgg gcaaggaagg
     3001 ggtgggggga aggaattgcc gcccgactgc aaccgcccga agggcggtct aggcagggac
     3061 tctgcgacca gaaaagtggg gtcataccac aggctcgaac ggaggcacaa agcggagtga
     3121 agtaggactt gggaatatga ggg
//
LOCUS       DATDSU010000096         9567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1276912, whole genome shotgun sequence.
ACCESSION   DATDSU010000096 DATDSU010000000
VERSION     DATDSU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276912"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..826
                     /locus_tag="VI981_03215"
     CDS             122..826
                     /locus_tag="VI981_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY4479339.1"
                     /translation="MSRNIDISIGEFYHIYNRGTEKRKIFLDDSYFERFVALLYLCNS
                     SFPIVMRRYQGQALAEIMASFERGEALVDIVGYCLMPNHFHLILREKRDRGISKFMLK
                     LLTAYSSYFNIRNDRSGTLFQGRYGAKHLHDDQYVKYCFSYIHLNPVKLLEPYWDVKG
                     IENIPNALDFLGRYEFSSYKDYIGQNRPCGRLLRKDAFPDFFNTPVDIRGEIKNWLNY
                     KNNFDYQGQALVGEHE"
     gene            819..1598
                     /locus_tag="VI981_03220"
     CDS             819..1598
                     /locus_tag="VI981_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY4479340.1"
                     /translation="MSEFIAIAVFVFGTIVGSFLNVVSLRYNTGLSIRKGRSKCFSCG
                     YALAWLDLIPILSFLFLGGRCRKCQSKISLQYPLVECATGLLFVFTFLVESNPPLLLI
                     LWIAFSLLVVIFVYDLKHKIIPDALVYAFDALGLIFLVYGVYRAQGHMPSAHELLAGV
                     YLALPFALLWVVSKGRWIGLGDAKLALGMGWFLGLASGFTAIVLSFWIGAVVSVGILL
                     VSKILGQHKFSMKSEIPFAPFMIISFLIVYFFTFTIFPIPF"
     gene            1710..2342
                     /locus_tag="VI981_03225"
     CDS             1710..2342
                     /locus_tag="VI981_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY4479341.1"
                     /translation="MFFRESNQTKNGRTGGFTLIEAVISIGIFVLFASIVLLRYGDFQ
                     GSILLTNLAYDVALQIRQAQVYGIAVREGEDSVGTKTFKRAYGIHFDLATDTKYLAFS
                     EPSSLGPGSDRKYDPADVNDINEREYNLNGGYLIKDLCVDVAAGTLCGADGVDIVDIV
                     FVRPNLGALIYYKQGLTEGTATNAKIEFASKSGGVRSVTVRTTGQISVRE"
     gene            2378..3061
                     /locus_tag="VI981_03230"
     CDS             2378..3061
                     /locus_tag="VI981_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479342.1"
                     /translation="MKRKFKKSGGFTRLVEDGVAPKDFWKSILHAINLPVNLLLRFIS
                     KGRKKSTPSFTTGFMLIEVIVATTLFVVVMTVAATAILGVVDATRKAQAISTIMTNLH
                     FVLEDISRQVRAGELSACTGGSPCTQIDIDQQLRVVTYAYDATNKKITQQINDAINGL
                     GPLLDLTSPQEVVLEDVEFYVSGVGDDNLQPRVLIVIRGHTVGKENVQSPFNLQTSVT
                     QRIDEIADF"
     gene            3302..3925
                     /locus_tag="VI981_03235"
     CDS             3302..3925
                     /locus_tag="VI981_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479343.1"
                     /translation="MQKGRKKSTPSFTTGFTLIETFVAILILTLAITGPMSIAQKGLQ
                     SSILAKDKTTAAYLAQDAIEFIRSIRDSNFYVGASGGGGGNWFDNLSGKCENPADYCI
                     VDTTLSSLDPNAVINCGGIYNCLPLNIDKIGGSYRYGYDASWDVTKFTRRVRVIRQPV
                     YADETDPDALIDQVPEALVDVWVSWRTGIIDRDYYLRTTIMNMKGSF"
     gene            3928..4587
                     /locus_tag="VI981_03240"
     CDS             3928..4587
                     /locus_tag="VI981_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479344.1"
                     /translation="MVTTHKKGFTLLYAVLIGSILLTIGTSIYNVSIKQVNFISLAGE
                     SVAAIYAAEAGFECAQYWNRKHPNGSAFSTTPPEKAIMCNGTKIGTYVDPNTDHDDDG
                     DSNGDESFTIHQTIPGTGETSVIGGKGGTDVCNRELPLFDPESLDCLSIFYFELPDPS
                     PGGRGSSSVDPITPCVIVYTREEQSASEGLYHLFTARAYNTCDLNDPRRLERALVNIK
                     I"
     gene            4715..5311
                     /gene="cas2"
                     /locus_tag="VI981_03245"
     CDS             4715..5311
                     /gene="cas2"
                     /locus_tag="VI981_03245"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEY4479345.1"
                     /translation="MNKKIGKIQKKVFTTLLMGIAFSLSRSPRQQWKIITDDLPKELE
                     KINIQNLEYGLHRLVSGQYIFYKNIGSGLYEACLTPAGQEYAKTLNLRNLVIAKPKRW
                     DQKWRVVFFDIPEKLRRKRNLFRDNLKALGFMEVQRSAFCHPYPCGEEIAALVNNYKL
                     RACVEYIVAEELSNKNYYLKKFNLYPVRSRTPRGLPRG"
     gene            5551..7623
                     /gene="ligA"
                     /locus_tag="VI981_03250"
     CDS             5551..7623
                     /gene="ligA"
                     /locus_tag="VI981_03250"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY4479346.1"
                     /translation="MAKKVPKEIIARLEKLRKTIDHHRYLYHVLDREEISAEVLDSLK
                     KELVDIEREYPELITPDSPSQRIGGKPLAEFKKIVHKMPQWSFNDAFTSEDISSFDAR
                     VKRFLKQRPDLFPDSRLRRASDGQARSDLEIDYTCELKIDGLKIVLEYEKGLLRSAAT
                     RGDGKVGENVTENVKTIESVPLRLNRPVDIIVEGEIWMSKREFEKQNKERAKRGEPLY
                     ANPRNVAAGSIRQLDPKIVKERNLKTFIYDIAQSSDDIPDTQAEELKYLDTLGLKVNR
                     NYIVCNSVDKMVEYWNTWQKRAPKEDYLIDGIVVKVNEKRYQDALGYTGKAPRFGIAF
                     KFPAEQVTTVVEDITLQIGRTGVLTPVAHLRPVLVAGSTVSRATLHNEDEIRRLDVRV
                     GDTVILQKAGDVIPDIVKVLTEMRTSKEKPFKWPQFVEACGGEGKIERIPGQAAWRCV
                     NKNSFAQQRRKLYHFVSKKAFDIDGLGPKIIDSLLANNLISSASDFFELKYGDLNNLE
                     RFAEKSASNIIESIEKAKKTTLSRLIIALSIPQVGEETAHDLAERFKTIRALQDASYD
                     DLEKIEGIGPVVAKSITLWFADTANKKLLNNITKHVTVEKNSARRQFKKEFENKTFVL
                     TGTLTSMSRDDAKAKIKSLGGSISGSVSSKTDYVVAGENAGSKFEKAETFGVRILSEA
                     QFLAMLEK"
     gene            7728..8012
                     /gene="gatC"
                     /locus_tag="VI981_03255"
     CDS             7728..8012
                     /gene="gatC"
                     /locus_tag="VI981_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY4479347.1"
                     /translation="MLSIKEIEKLAALSRIKLTDAEKDKFQKEIESILSYVNQIKEVS
                     AKLPEAPLPETRNVFRDDRDALLSGEFTDSILKNAPQSEAGYLKVKKILT"
     gene            8009..9424
                     /gene="gatA"
                     /locus_tag="VI981_03260"
     CDS             8009..9424
                     /gene="gatA"
                     /locus_tag="VI981_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY4479348.1"
                     /translation="MIETNGLTIVSAREHLDHGDFRAVELAEAYLKNIEAKNKTINAY
                     LEVFDDVVEQAKEADKKIQAKKSGTLTGIPIAVKDNILIKGRRAGSASKILEGFVAPY
                     DATVIEKLKKVGAVFLGRTNMDEFAMGGSNENSAYGPVKNPHDLSRVPGGSSGGSAAA
                     VAMDGALAALGSDTGGSVRQPASFCGVVGLKPTYGGISRHGLMAMAASLNQVGTLAKT
                     VEDTKLLFDVIRGKDPMDSVSIDLAYSNESKKITVGVPWHFMSEGVDPDVLTNFNESI
                     KQLEKKGCTIREISIPHISYSLAIYYVLMPAEATSDLARFDGVKYGMRKEGNGLLGDY
                     LETRTVGFGAEVRRRIMLGNYILSSGYYDAYYNKANLVRQLLRKDFEEVFRGVDVIMT
                     PTSPVPAFKIGERASDPLSMYLADIFTVTANLVGGPAISIPSGFANRDGKNLPLGLQF
                     TAPHGREDLLFQVSHLLSSTV"
BASE COUNT         2766 a         1997 c         2210 g         2594 t
ORIGIN      
        1 tctagcgctc gcccccgacc ccgacaaggc caggccttgg cgggggacat cggagctttt
       61 accaaggcct ggccttggta aaaaatgaaa aattgaaagc ccagacagta ataggacgaa
      121 aatgagcaga aatattgata tatcaatcgg cgagttttac cacatctaca accgaggcac
      181 ggaaaaacga aagatttttc ttgatgattc ctattttgag cgatttgtgg cactgttata
      241 tctttgtaat tcgtcattcc caatcgtaat gcgaagatac caaggccagg ccttggcaga
      301 gattatggcg tcattcgaga gaggagaggc gttggtcgat attgtcggat attgtctcat
      361 gccaaaccac ttccacctga ttttgcgcga aaaaagggac cgcggtattt ctaaattcat
      421 gctgaaactt ctgacggcat acagtagcta tttcaacatt cgaaacgacc gatctggaac
      481 tttgtttcaa gggcggtatg gagcaaaaca tttacacgac gatcagtatg tcaaatattg
      541 tttttcatac atccacctaa acccggtcaa acttttggag ccatattggg acgtaaaagg
      601 tattgaaaat attccaaacg cactcgactt tcttggtcga tacgagtttt caagttacaa
      661 agactatatt ggacagaatc gaccgtgcgg aaggttgcta aggaaagatg ccttcccgga
      721 tttttttaat acccctgttg atattcgggg tgaaattaaa aattggctga attataagaa
      781 caattttgat taccaaggcc aggccttggt tggagaacat gagtgaattt attgctattg
      841 cggtctttgt ttttggcacg atcgttggaa gtttcctaaa cgtcgtgtct cttcgataca
      901 acaccggcct ttcaatcaga aaaggtcgct ccaagtgctt ctcgtgcggc tacgcgctcg
      961 catggcttga tctcataccg atactaagtt ttctctttct cggcggtagg tgtcgaaaat
     1021 gccaaagtaa aatctctctc caatatcctt tagttgagtg tgctacgggg cttcttttcg
     1081 tgttcacttt tctcgtcgaa agtaatccac cgttgctgct cattttatgg atagctttct
     1141 cacttctggt tgttattttt gtctatgatc tgaaacacaa aattattcca gacgcactcg
     1201 tgtacgcgtt cgacgcgctc ggacttattt ttctcgtgta tggtgtatac cgagcgcaag
     1261 gccatatgcc ttccgcacat gaactactgg ccggcgtcta tcttgctctg ccttttgcgc
     1321 tgttgtgggt tgtctcaaaa gggcggtgga ttgggcttgg agacgctaaa cttgccctcg
     1381 gaatggggtg gtttctgggt cttgcgtctg gttttacggc gatcgtgctt tcgttttgga
     1441 ttggtgcggt cgtaagcgtc ggaattttac ttgtctctaa aatactggga caacacaagt
     1501 tttcaatgaa aagcgaaatt ccgtttgctc cgtttatgat aatttccttc ctgatcgtat
     1561 attttttcac ttttaccatt ttcccaattc cattttaaat tactttacca aggccaggcc
     1621 ttagcataac tcatatgcta aggcctggcc ttggtaaaag gtgccaattt tccctgtcct
     1681 cctgcgtaaa aaagcctata atgtcaacta tgttctttcg ggagagtaat caaactaaga
     1741 atggccgaac aggcggtttt acccttatcg aagcggtgat ctcaatcggt atttttgtgc
     1801 tgtttgcgag tatagtacta ctgcggtatg gagattttca gggaagtatt ttgctcacta
     1861 atcttgcata cgacgtcgca ttgcagatac gccaagctca agtctacgga atagctgttc
     1921 gtgagggtga agacagtgtg ggcacaaaaa cttttaagcg ggcctatgga attcattttg
     1981 accttgcaac tgatactaaa tatttagctt tttccgaacc ctcgagtctc ggcccaggct
     2041 ctgacagaaa atatgaccct gctgatgtaa atgacattaa cgagcgagag tacaacctaa
     2101 acggcggcta cctgataaaa gacctatgtg tggacgtggc cgcagggacg ctttgcggtg
     2161 cggacggtgt ggatattgtc gatattgttt ttgttcgtcc aaatcttggc gcgcttattt
     2221 actacaaaca agggctcaca gagggcactg cgacaaacgc caaaattgaa tttgcatcca
     2281 aaagtggagg tgttcgatca gttactgtcc gcacaacggg ccaaatttcg gttagggaat
     2341 agtttatgga tcgcttttag cgaataggtt catacgaatg aaaagaaaat ttaaaaaaag
     2401 cggagggttt acccggttag tagaagatgg cgtcgcgccc aaagattttt ggaaatctat
     2461 tctgcatgct atcaatttgc ctgtgaattt gttgctaagg tttatctcta aagggcgtaa
     2521 aaaatcaacg ccatctttca ctaccgggtt catgttaatc gaagttattg tcgccacaac
     2581 gctttttgtc gttgttatga cagttgccgc cacagccata ctcggtgttg ttgacgcaac
     2641 gagaaaagcc caggcaatct cgaccattat gacaaatttg cacttcgtac tcgaagatat
     2701 ttcacggcaa gtaagagccg gggaactctc tgcatgcacc ggcggtagcc cgtgtacaca
     2761 aattgacatc gatcagcaac tgcgcgtcgt tacatacgca tacgatgcga caaataaaaa
     2821 aattactcaa cagattaatg atgcaattaa cggtcttggc ccgctgttgg atctcacttc
     2881 tccccaagag gttgtgctgg aagatgttga attttatgtg agcggtgtcg gtgatgacaa
     2941 cctccaacct cgggtactca tagttattcg cggtcataca gttggcaagg aaaatgtgca
     3001 gtctccattt aatctccaaa ccagtgttac tcaacgtatc gacgagatcg ctgactttta
     3061 gcatgaaacg ccaaactcta aataaaaaaa ggggctttac cctgaagcga tcacaccaaa
     3121 caatgaattt atgatcaatt ttttattaaa atcaaaacac cgaatttcgt ctacaaagcg
     3181 atctgtttcg agatctactt cagggtttac ccggttagta gaagatggcg tcacgcctaa
     3241 agattttcgg gtattttttt ccaaaatttt caacttgcca atagatctgt tatttagata
     3301 catgcaaaaa gggcgcaaaa aatcaacgcc atctttcact accgggttta ctttgatcga
     3361 aacatttgtg gcgatcctca ttcttacgct cgccataact ggtcccatga gtattgcgca
     3421 aaaagggctt caatcatcta ttctcgcgaa agacaaaacg acagccgcat atttagcgca
     3481 agatgctatt gaatttatac gctcgatcag ggattctaat ttttatgtcg gcgcgtctgg
     3541 cggcgggggt ggaaattggt tcgacaattt atccggaaag tgtgaaaatc cagcagatta
     3601 ctgtatcgtg gatacgacgc tctcgtcact tgatccgaat gcggtcatca actgtggagg
     3661 catctataac tgcctgccgc taaatattga taaaattggc gggagttatc ggtatggata
     3721 cgacgcctcg tgggacgtga cgaagtttac aagaagagtg agagttattc gacagcccgt
     3781 gtatgctgac gagacggatc cagacgcact tatagaccaa gtccccgaag cgctagttga
     3841 cgtttgggtg agctggagga cgggcatcat cgaccgagat tactatctac gtacgacgat
     3901 tatgaatatg aagggaagtt tttaactatg gttactacac ataaaaaagg atttaccctt
     3961 ttgtacgctg tgcttattgg cagtattctt ttaacgattg gtacttctat ttacaatgtc
     4021 agtattaaac aggttaattt tatttctctc gccggagaat ctgtggcagc gatttatgcg
     4081 gctgaagcgg gattcgagtg tgctcaatac tggaacagaa agcatcccaa cggatcagca
     4141 ttttctacca ctcctcctga aaaagcaatt atgtgcaacg ggacaaaaat tggaacttac
     4201 gtcgatccaa ataccgatca tgacgacgat ggagacagca atggtgacga aagttttacg
     4261 atccaccaga cgattccggg aacaggcgaa acaagtgtca tcggggggaa agggggtacg
     4321 gacgtgtgta atcgtgaatt gcctctattc gatcccgaaa gtcttgactg tcttagtatt
     4381 ttctattttg agcttccaga tccaagcccc ggcggccgcg gttcatcaag cgtcgaccct
     4441 attactccat gcgtcattgt ttatacgcgc gaggaacaaa gtgcttcaga aggtctctac
     4501 cacttattta ccgctcgcgc atataacacc tgtgatctaa atgatccaag gcgactggag
     4561 cgcgcactcg tgaacatcaa aatttagcct cctttatcac gatttgccaa catggttcgg
     4621 gtccatggtc cgcgcagctc accccttcac ctagtaaata ttatatagaa acgatcgtcc
     4681 caatatcata aattaatcgg agaagaaagg aataatgaat aaaaagattg gaaaaattca
     4741 aaagaaagtt tttacaacat tgcttatggg aattgctttc agtttaagca gatctcctcg
     4801 tcaacagtgg aaaataatca ctgatgactt accaaaagag ctagaaaaaa taaatataca
     4861 aaatctcgaa tacggacttc ataggttggt ttctggtcaa tacatatttt ataaaaatat
     4921 agggagcgga ttatacgagg cctgtcttac acctgctggg caagaatatg ccaaaacact
     4981 gaacttgcga aatcttgtaa tcgcaaagcc aaaacggtgg gaccaaaaat ggcgcgttgt
     5041 tttctttgac attcctgaaa aactaagacg taagcgaaat ttatttcgcg acaatttaaa
     5101 agcactcggt tttatggagg tacaacgttc tgcgttctgt cacccatacc catgtgggga
     5161 ggaaatagcc gcattggtta ataactacaa gttgagagct tgtgtcgaat atattgtggc
     5221 tgaagagcta agcaataaga attactatct aaaaaaattt aatctttacc ccgttagaag
     5281 cagaacgccc cgcggcttgc cgcggggatg aatgcaattt gattttagcg aacacggggt
     5341 ttaatgcccc gtgtgagctt ctaacggggt caactaaaaa attaagacct cacgtcaatt
     5401 tatcactttg taatattcaa acgatcgtca cgatattata atttcagggg aacgatgtaa
     5461 attctataca atagtacaaa tatctttagt tcaaatctcc atagcacaaa tctccttgcc
     5521 cagcgttttt tgaagaagta tactctcgac atggctaaaa aagtgccgaa agaaataatt
     5581 gcccgtctcg agaaattgcg gaaaactatt gaccaccata ggtatcttta ccatgtgctt
     5641 gaccgtgagg aaattagcgc agaagtgctc gattcgttaa aaaaagaact tgtcgatatc
     5701 gagcgcgaat acccagaact cataactcca gattctccgt cacagcgtat cggcggaaaa
     5761 ccgctcgctg aatttaaaaa aattgttcat aaaatgccgc aatggtcttt taatgacgca
     5821 ttcacgtcag aagatatttc aagttttgac gcgcgtgtta agcgtttttt gaaacaacgg
     5881 ccagaccttt tccccgattc ccgccttcgc agagcttcgg acggtcaggc aaggtcggac
     5941 cttgagattg attatacctg tgagcttaaa attgacggtc tcaagattgt gcttgagtat
     6001 gaaaagggac tgctccgaag cgctgccacg cggggcgacg gcaaggttgg cgagaatgtc
     6061 acagagaatg taaagacgat tgaatcggtg ccgcttcgcc tcaacaggcc agtcgacata
     6121 attgtcgagg gagaaatttg gatgagcaaa cgcgaatttg aaaagcaaaa taaagaacgt
     6181 gcaaaaagag gcgagccgct ttatgcaaat ccgcgcaatg ttgccgcggg ttcaatccgc
     6241 cagcttgatc cgaagatcgt taaagagcgg aacttaaaaa cattcattta tgatatagcg
     6301 caatcgagtg acgatattcc agacacccaa gccgaggaac tcaaatatct tgatacgctt
     6361 gggctcaagg tcaatagaaa ctatatagtg tgcaattctg tcgacaagat ggttgagtat
     6421 tggaatacat ggcagaaacg cgctccaaaa gaagattatt taattgacgg aatagtcgta
     6481 aaagtaaacg aaaaaagata tcaggacgcg ctcggctata cggggaaagc gccgcgtttt
     6541 ggcatcgctt ttaaatttcc agcggaacaa gtaacgacag tcgtagagga tataaccctc
     6601 cagattggac gcaccggggt tctcacgccg gtagcgcatt tgcgtcccgt acttgtggcg
     6661 ggctcgacgg tttctcgcgc cacgcttcac aacgaagatg aaatacggcg tcttgatgtt
     6721 cgtgttggag ataccgtaat tctccaaaag gcgggagacg ttattcctga tattgtgaaa
     6781 gtcctcaccg aaatgcgaac aagcaaggaa aagccattta agtggccgca gtttgtagag
     6841 gcctgcggcg gcgaaggcaa aattgagcgt attcccggcc aggcggcgtg gcgctgcgtc
     6901 aataaaaatt cttttgccca acagcgaaga aaactctatc atttcgtgtc taaaaaagct
     6961 ttcgatattg acgggctggg tcctaaaata attgattcac ttcttgccaa caatcttatt
     7021 tcttcggcgt ctgacttttt tgaattaaag tatggagact tgaacaactt ggagcgattt
     7081 gccgaaaaat cggcaagcaa tatcatcgag tcgatcgaaa aagcgaaaaa gacaacatta
     7141 tcgcggctca ttatcgcgct ctctattccg caagtcggag aggagacagc gcacgatctt
     7201 gcggaacgct ttaaaacgat acgagcactt caggacgcct cgtatgacga ccttgagaag
     7261 atcgaaggta taggtcctgt cgtggcgaaa tctataacgc tctggtttgc cgacacagca
     7321 aacaaaaagt tgctgaacaa catcacaaag catgttacgg tggaaaaaaa ctctgctcgc
     7381 cgtcaattca aaaaggaatt cgaaaataag acatttgtcc ttacgggaac gcttacttcg
     7441 atgtctcgag atgacgcaaa agcaaaaatc aaatctctcg gcggctctat ctccggatct
     7501 gtttcttcaa aaactgatta tgttgtggct ggagaaaatg caggttctaa atttgagaag
     7561 gccgaaacat tcggggtgcg aattttgagc gaagcgcaat ttcttgctat gcttgagaaa
     7621 taaacatacc aatatacaaa ttttacgaat aatacgaata atactaatta tcaaaatatg
     7681 ctattcgtat ccatttgtat attcgtatcc attagtatat ttgtatcatg ctaagcataa
     7741 aagaaataga aaagcttgca gccctgtcac gcatcaaact gactgatgcc gaaaaagata
     7801 agtttcaaaa agaaattgaa tcgattttgt cgtatgtcaa tcagataaag gaagtttcgg
     7861 ctaagttgcc agaagcaccg ctacctgaga ctcgaaatgt ctttcgtgac gaccgagatg
     7921 cacttttgtc cggagaattt acagattcca tactcaaaaa tgcgccgcag tcggaggccg
     7981 gatatctgaa agtcaaaaaa atactgacgt gattgaaacc aatggactta caattgtgag
     8041 cgcgagggaa cacctagacc atggtgattt tcgagcagtt gaactggcag aggcatatct
     8101 taaaaatatc gaagcaaaga acaaaactat aaatgcatac ctggaagttt ttgatgatgt
     8161 agttgagcag gctaaagaag cggataaaaa aattcaggcc aaaaaatcgg gaactcttac
     8221 cggaattccg attgccgtta aagataatat tttaattaaa gggcgtcgcg ccggttcggc
     8281 ttcaaaaatt cttgaaggat tcgtggctcc ctatgatgcg actgtgatcg agaaactgaa
     8341 aaaagtggga gccgtttttc ttggccggac aaacatggac gagtttgcca tgggaggttc
     8401 gaatgaaaat tcggcatacg gaccagtgaa aaatccgcat gacctttctc gtgttccggg
     8461 tggttcatct gggggaagtg cggccgctgt tgcgatggat ggagctcttg ccgctcttgg
     8521 atccgataca ggcggctctg taaggcagcc ggcaagcttt tgtggcgttg ttggtctaaa
     8581 accaacgtac ggtgggatct cgcgtcatgg tcttatggct atggcagctt ccttaaatca
     8641 ggttggaaca ctcgcaaaga cggtcgaaga taccaagctg cttttcgacg tgatccgagg
     8701 taaggatcct atggatagtg tctcaattga tctcgcctac tcaaacgagt ccaaaaaaat
     8761 tacagtaggt gtaccgtggc attttatgag tgagggagtg gatcctgatg tacttacaaa
     8821 tttcaatgag tcaataaagc aactggaaaa aaaaggttgc acgattagag agatatcaat
     8881 accgcacatt agttactctc tcgctatata ttatgtactg atgccagcgg aggctacatc
     8941 tgacctggcc cgttttgatg gcgtgaagta tggaatgcga aaggagggta acggactgct
     9001 cggtgattat cttgagacgc ggacagttgg ttttggtgcg gaagtgcgca gacgaattat
     9061 gctcggtaac tacattcttt cttccggcta ttacgacgca tattacaaca aagccaatct
     9121 tgttcgtcag ctcttgagaa aagatttcga agaagtattc agaggtgtgg atgtcataat
     9181 gacaccaaca tcaccagtac cggcatttaa aatcggagag agggcttccg atccactttc
     9241 tatgtatctg gccgatatat ttacagtgac ggcaaatctt gtcggaggtc ctgcgatttc
     9301 tattccatcg gggtttgcaa accgtgacgg aaaaaattta ccgctcggac tacagttcac
     9361 ggcgcctcac ggcagggaag atctcttgtt tcaggtgagt cacttgctct caagtacagt
     9421 ttaagtactg ttcgttgaaa agaaacggtt ggaaattgat aattactaaa tataatggac
     9481 gacgacgaaa aaaaaccagg attgacgtgg gatgccgttg agatcggatt tgtcttatta
     9541 atcgctgtgc tcgtaatcca aggtctt
//
LOCUS       DATDSU010000097         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2109734, whole genome shotgun sequence.
ACCESSION   DATDSU010000097 DATDSU010000000
VERSION     DATDSU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109734"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VI981_03265"
     CDS             <1..658
                     /locus_tag="VI981_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEY4479349.1"
                     /translation="LVSGNPDGTYEPAENINRAEFLQLAMNVYLYLNTDMESEYNILA
                     DGTATDYYADLDTSAWYAGVVTAATDWDFVSGSDCDEDKCFNAGSSITRAEATKILYN
                     MFLDMLAYEGVTMELDLDSQTDDGISGTATLTQTGGRTWVSVMLDVYGESDHPAHIHA
                     GDCEDTGDIEWSLEDVVAGSSTTELEVSMATILDNLPLYLNVHMSEDEMDTAVACGNI
                     "
     gene            777..1907
                     /locus_tag="VI981_03270"
     CDS             777..1907
                     /locus_tag="VI981_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4479350.1"
                     /translation="MKYKRILFKSLYQNIGKKPITVLYGARQVGKTTLTKSLMEKFSG
                     ALYLQGDDPKDAALLEQKSAKELSQLVSGYKLIVIDEAQRIQNIGLTLKLLADNITGV
                     QVLATGSSSFELANKLSEPLTGRNRKFHLYPLSIKELVDAYGQITVQKSLEDLLSFGM
                     YPQIVNASARKDKILLIKELTGDYLFKDLFFLGDIRNSFAFEKLVKLLALRVGSEISY
                     PELAKEVGVSRDTIYSYITLLEQAFITFRLTPLYTNKTKEINKSHKIYFYDTGIRNAL
                     IGNSDPLGVRPDKGAIFENFFIAEKIKERAYEMRLSEIHFWRSRQGAEIDFIESADSN
                     KGISAYECKWKKKTEAPLLFKKIYPKAQFKTITMENIVSHFI"
     gene            complement(1908..>2397)
                     /locus_tag="VI981_03275"
     CDS             complement(1908..>2397)
                     /locus_tag="VI981_03275"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY4479351.1"
                     /translation="EAAKCGDLFITVTGNRDVLTSANVRLMKNGAILANSGHFDCEID
                     VKGIRSRSLRSRVLRPFLEEFVLRGGWKVIYVLAEGRLVNLSAAEGHPSEVMSMSFCG
                     QVLACEYLVKNHKKLGKKVYALPSTVDDTIAALQLETMGIQIDSLSAKQKKYLSSWQT
                     GT"
BASE COUNT          718 a          541 c          527 g          611 t
ORIGIN      
        1 tcttgtttcc ggcaatccgg atggaaccta cgaacctgcg gagaacatca accgcgccga
       61 attcctgcag ctcgccatga acgtgtacct ctatttgaac acggatatgg aaagtgaata
      121 caacattttg gcggacggaa cggccaccga ctactacgcc gatttagaca cttcagcttg
      181 gtacgccggt gtggtaacgg ccgctaccga ctgggacttt gtttccggca gcgactgtga
      241 cgaagacaaa tgtttcaacg ccggtagctc catcacacga gcggaagcca ccaagatttt
      301 atacaacatg ttcttggata tgcttgcgta cgaaggggtg acgatggaac tcgacctcga
      361 ctcacagacg gacgatggca ttagcggaac ggcgaccctc actcaaacgg gtggccgcac
      421 atgggtttcg gtgatgctgg acgtatacgg cgaatccgat catcccgcgc atattcatgc
      481 cggagattgt gaagacaccg gagatattga atggtcactt gaggacgtag tggctggatc
      541 ttccaccacc gagcttgaag tgagcatggc aacgattctg gacaatcttc ctctctactt
      601 gaacgtacat atgtctgagg acgagatgga cacggctgtg gcctgtggaa atatttgaaa
      661 caggatgaaa tcctagtttc aaatctccct tataaacaaa aaagccgacc tccgaaaagg
      721 ggccggcttt taaaatatat cttgcatttt gagcgcttat tttgtatact atactcgtga
      781 aatataaaag aatactcttt aagtcgctgt atcaaaatat aggcaaaaaa cctattacag
      841 tcctctatgg agcgagacaa gtggggaaaa caacactgac aaaatcattg atggaaaagt
      901 tttccggcgc actttattta caaggagatg atccgaaaga tgccgctttg ctggaacaaa
      961 aatcggcaaa agagctttca cagctggttt cggggtataa acttattgtt attgatgaag
     1021 ctcaacgtat acaaaatata ggcctcaccc tgaaactctt ggccgacaac ataacgggag
     1081 tgcaggtgct ggcaacaggc tcttcttctt ttgaattggc caataaatta agtgagccgc
     1141 ttacaggcag aaatcgaaaa tttcatttat atcctctatc cattaaagaa ctggtggatg
     1201 catacggaca aataactgtt caaaaaagtt tggaagatct cttgagtttc ggtatgtatc
     1261 cgcaaattgt taacgcttct gcacgaaaag ataaaatact tcttattaaa gaactaacgg
     1321 gagattatct gttcaaagac ttatttttcc ttggagacat ccgaaattcg ttcgctttcg
     1381 aaaaattggt aaagttattg gcgctaagag tgggcagtga aatttcttac cccgagttgg
     1441 ctaaagaagt aggtgtgagc agagacacga tttatagcta cataaccctc cttgaacaag
     1501 cttttatcac ctttcgatta actcctctgt acaccaacaa aaccaaagag atcaataaaa
     1561 gtcacaaaat ttacttttat gatacaggca ttcgaaacgc actgattgga aacagtgatc
     1621 ccttaggggt cagacctgat aaaggggcta ttttcgagaa tttttttatt gcagaaaaga
     1681 taaaagaacg tgcctacgaa atgagactga gcgagattca cttttggaga agtagacagg
     1741 gcgcggaaat agatttcatt gaatctgcag attcgaacaa aggaatctca gcttatgaat
     1801 gtaaatggaa gaaaaagacg gaggcacctc ttctattcaa aaaaatctat ccgaaagctc
     1861 aatttaagac cattactatg gagaacatag tgtcacactt catttaatca cgttcccgtc
     1921 tgccacgaac tcaaatactt cttttgtttc gcactcagcg aatcaatttg aatgcccatt
     1981 gtctccaatt gcagagccgc aatggtgtca tccaccgtgc tcggcagcgc gtaaactttc
     2041 ttgcccagtt tcttgtgatt tttcaccaaa tattcgcagg cgagcacctg accgcagaaa
     2101 ctcatggaca tcacttcgct ggggtggcct tcggcggcgg aaaggttcac aagtcggcct
     2161 tcggcaagaa cgtaaatcac tttccagcca cctcgcaaaa caaattcctc caaaaatggg
     2221 cgtagaacac gcgagcgaag agagcggctc ctaatccctt tcacatcgat ttcgcagtcg
     2281 aaatgaccgc tattcgctaa aattgcacca ttcttcataa gccgcacatt ggcggacgta
     2341 agcacatctc tgtttccagt gacggtgatg aacaaatccc cacattttgc cgcctca
//
LOCUS       DATDSU010000098         4488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2110006, whole genome shotgun sequence.
ACCESSION   DATDSU010000098 DATDSU010000000
VERSION     DATDSU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110006"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VI981_03280"
     CDS             complement(<1..516)
                     /locus_tag="VI981_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479352.1"
                     /translation="MIILGIETSCDDTAVSLVKGSGGRRRPRFQILAQVVSSQTAAHA
                     PWGGVVPNLAKREHAKNLIPVLKEALAKARLIRGSTSYFRLKVESCRKILAREPELLK
                     QFLAVIPTLAPPKINAIAVTVGPGLEPALWTGINLARALALVWKKPVIPVNHLEGHIV
                     SVLAPRGRLLGQ"
     gene            complement(1446..2105)
                     /locus_tag="VI981_03285"
     CDS             complement(1446..2105)
                     /locus_tag="VI981_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479353.1"
                     /translation="MDYLNRLRYAQSQAGDLTGKAGAEAAYEVAQCLGGCRISGTTIP
                     NEEDGTLDFELALKAAQHARWLTQRIKQRVSQLISSQNEDVESDDEFYESDCRASAVL
                     MRHELWCAMHAIHEADDVLSRANEGKAELTDELTLLIEELTDLDALMIQNIGIFGCVA
                     DLPLLTNLKAKVKTESLSELEPLPWWLDGTIEKAAEQAMKKLMEWLPKPSEIERKLRC
                     R"
     gene            complement(2370..2930)
                     /locus_tag="VI981_03290"
     CDS             complement(2370..2930)
                     /locus_tag="VI981_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4479354.1"
                     /translation="MGNPLEMLEVIDENDNVVGLENRAKIHQDRLLHREIHIWFLTPK
                     SEIVFQHRAKNKDTYPDKLDATVGGHVGPKMSYEETTVKECKEETGIDIDLNKLVFIK
                     KMKKKSFDEATGLTNNTIRSQYAYLYDGSINDLQIEDGKSQGFVAWKIDELSHLSETD
                     KNKFIPLILEQDMLDIFNQARSIFKL"
     gene            complement(3147..>4488)
                     /locus_tag="VI981_03295"
     CDS             complement(3147..>4488)
                     /locus_tag="VI981_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY4479355.1"
                     /translation="DRLARNSVDGGKIIYLVDINRIASLRFPQFWFEPTPQGKFMLQV
                     AFGQSKYFSDNLVENVKRGFRQKLRRGEWLTKAPYGYVNNLKTKNIEPHPTYSKIVTR
                     AFVEYATATYTIESLAKFLAELGLTTHTGTPLGKASIVRMLTNRAYLGFIKHHDEWYQ
                     GSFAPILSPTLFEAVQKVLQTKRRPRHSKVRYDFPFVQFVRCGECGGMITAQYTTNRF
                     GTKYTYYHCTKKHGVCHQPYVAADSIASQIKSTLQSVSLPLEEIEKMEKLITTWESES
                     ISSSGTVAQNLKSKLSETQEKLDKLISVYLDDDIERPIYLERKDLLLRQKMSIEESLR
                     DFGQQRKNWIEPLRSFVLSLREAVELEKTDNHLEWKRFFQKIGSNPSLNDKTLSIGWG
                     ELWEFTASAIGGKETDSAILASAKSLRYCNFDNVSLGELIISFARILHERKQDE"
BASE COUNT         1139 a         1068 c          991 g         1290 t
ORIGIN      
        1 ttgccccaag agtcgtcctc ggggagccag caccgacacg atatggccct ccagatgatt
       61 gactggaata accggttttt tccaaactag cgccagcgcc cgcgccagat tgatgccggt
      121 ccagagcgcc ggttccaatc cagggccgac cgtaacggcg atggcgttaa ttttcggcgg
      181 cgccaaagtc ggaatgactg ccaaaaattg tttcaataat tccggctcgc gcgctaaaat
      241 ttttcggcaa gattcaacct taaggcggaa atacgaggtc gaacctctaa ttaatcttgc
      301 tttcgccaac gcttccttca gcactggaat caggtttttc gcgtgttcgc gtttggccag
      361 attcggcacc accccgcccc acggcgcatg cgcggcggtc tgcgaactga ccacctgcgc
      421 caggatttga aatcgcggcc gccgccgacc accgctccct ttaacaagac tgacagccgt
      481 atcgtcgcag ctggtttcaa tgccaaggat gatcataatt tttttaactc taacaaaagt
      541 cgccgcgatt cacaaaaaaa gagaccccgc agggtctcgc atcggatcat ctggtcaggt
      601 tatcatttgg ccggtccaga tggcgaccag tttatcactg accgagtgtt accgatgtat
      661 ctcatcatcc gcagtgtttt tattttggtt acgaagcact aattttatga actaatcata
      721 tcattcgctc gaattttgca cgaacggaaa caggacgaat gaacgccccg cccgcctccg
      781 ctaacgctac ggcggacacc tcggcaaaat ttcctttaca atttcggatt ttgcgcgcga
      841 ccaatttttc caattaagga aaaggaaatt ttgcctcggt gtttcgccct caagaattcg
      901 ggcgggcggg gcttcggaat ttcggcgtgc ctcgttgggt taagtaaaat tattgagaaa
      961 cgtttttaag gtgaaaaaga ttttactgtg aaaaactgaa agagtaaaat gataaagacc
     1021 agagtttcct ctggtcttta gagcgtttca ggctaccctc gtcaccttgc tcgcgttctc
     1081 gcgagtggca tggtggcagc ccttgttcgg ggattgcctc cacaccttgg gcatgtgacg
     1141 gaacgatgtt gctgctcaac tagggcagaa cagccattca cacgaactgg ctcgattgac
     1201 ccaaccgtgg ttcgacaaca gccattcaca caccgcttac ctgcttgggc taaaatgtag
     1261 ccagtgtcgt tgcatttcga acacatactt catgctcctc gtttgatttc taagatcaac
     1321 tatttcgacc ctttggggtc agcagatgta atctgctagt cctcacggac ttcatcaggc
     1381 aaaccacaca gtttgcaata gaagagtttt tttagaaggg tctcttaacc ttcataccgt
     1441 ttgcgctagc ggcatcggag cttgcgctcg atttcgctcg gctttgggag ccattccatg
     1501 agtttcttca tggcttgctc cgccgctttt tcaatggtgc catcaagcca ccaaggaagc
     1561 ggttccaact ccgagagcga ttcggtttta accttcgctt tcaggttggt aagcagagga
     1621 agatcggcta cgcaaccgaa gattccgatg ttctgaatca tgagcgcgtc cagatcggtg
     1681 agttcctcga tgagtaatgt tagctcatcg gtaagctctg cctttccttc gttggctcgt
     1741 gaaagaacat cgtcggcttc atggattgcg tgcatagcac accacagctc atgtcgcatg
     1801 aggacagcac tcgcccggca gtccgattca tagaattcgt catcggattc tacatcttcg
     1861 ttctgcgagg agatcagctg agacaccctc tgcttgatgc gctgtgtcag ccaccgtgcg
     1921 tgctgcgctg ccttaagggc aagttcgaaa tccagggtac cgtcttcctc atttggaatt
     1981 gtggtaccag aaattcgaca cccgccgagg cattgcgcaa cttcgtaggc agcttccgct
     2041 ccagctttgc cagtgagatc accggcttgg ctttgtgcgt atctcaaacg attgagatag
     2101 tccatggcgc tctccttcct ttttccccga ccatcttatt tccctcattt cagcgagcac
     2161 agtatgaact cgtcttatag aggtattcga caatcggctt aaatttccaa agaactgttt
     2221 tactgaacta ctagtatgct agcacagtga aagaaatctg tcaagcattt tacaagatgg
     2281 tgagaaatca tgcttaaaaa gctatattta gagccatatt ttggaataga gataaaatct
     2341 gcgggttatc aacaaaataa attttgtttt catagtttga aaatacttct cgcttgatta
     2401 aaaatatcta acatatcttg ttcaagaata agaggaataa acttgttttt atctgtttct
     2461 gaaagatgag aaagttcgtc aattttccaa gcaacaaaac cttgtgattt tccgtcctca
     2521 atctgtaaat catttattga accgtcgtaa agataagcat attgactgcg aatggtgttg
     2581 tttgttaaac ctgttgcttc gtcaaaactt ttctttttca tcttttttat aaaaacaagt
     2641 ttattcaaat caatatctat tcccgtttct tctttacatt ctttaacagt agtttcttca
     2701 tacgacattt tcggtccaac atgtccgcca actgtcgcgt ctaatttgtc gggataagtg
     2761 tctttatttt ttgctctatg ttgaaaaacg atctctgatt ttggagtaag aaaccaaata
     2821 tgaatttcac gatgaagcaa tctatcttgg tggattttag cacgattttc taaaccaaca
     2881 acattgtcgt tttcgtctat aacctccaac atttctaatg gattgcccat aaaatgattt
     2941 tatcattttt ctagtatttt ttctacttgc tagtcccgcc aaaaacgaat tccgaagccc
     3001 cgcccgcccg aattcttgag ggcgaaacac cgaggcaaaa tttccttttc cttaattgga
     3061 gaaattggtc gcgcgcaaaa tccgaaattg taaaggaaat tttgccgagg tgtccgccgt
     3121 agcgttagcg gaggcgggcg gggcgttcat tcgtcctgtt tccgttcgtg caaaattcga
     3181 gcgaatgata tgattagttc accaagagaa acattgtcga aattacaata acggagcgat
     3241 tttgcggaag ccaaaatcgc gctgtcagtt tcttttccgc ctatggcgga agccgtgaat
     3301 tcccaaagtt cgccccagcc aattgaaagt gttttgtcat tgagagaagg gttagagccg
     3361 attttttgga aaaaccgctt ccattcaaga tgattgtcgg ttttttccaa ttctacggct
     3421 tctctcaaag acaaaacaaa actccgcaag ggttcgatcc aattctttct ctgttgtcca
     3481 aaatctctca aactttcttc gattgacatt ttttgacgca ggagaaggtc tttgcgttcg
     3541 agatagattg gacgttcaat atcgtcatcg agataaacag aaattaactt gtccagtttc
     3601 tcctgcgttt cagaaagttt ggacttcaga ttttgggcaa cagttccgct tgaagaaatt
     3661 gactcacttt cccaagtggt aatcaacttt tccattttct caatttcttc aagcggaagc
     3721 gacactgatt gaagggtgct tttgatttga cttgcaatcg aatcggcggc gacatacggc
     3781 tggtggcaga ctccgtgttt tttggtacag tgatagtagg tgtacttggt gccgaagcga
     3841 ttggtcgtat attgcgccgt gatcatgccg ccacactcgc cacaacgcac aaattgcaca
     3901 aacggaaagt cgtagcgcac cttgctatgt cttggccgcc gtttcgtttg caagaccttc
     3961 tgcaccgctt caaacagtgt cggagaaaga attggcgcaa agcttccttg ataccattcg
     4021 tcgtgatgtt tgataaagcc gagataggca cggtttgtta acattcgcac tatggaagct
     4081 ttgccaagtg gcgttccggt gtgagtagtc aaaccgagtt cagccaaaaa cttcgccaag
     4141 gattcaatgg tataagtggc ggtggcgtat tctacaaaag cccgcgtgac aatttttgaa
     4201 tatgttggat gtggctcaat atttttcgtt ttcaaattgt tcacatagcc ataaggagct
     4261 tttgtgagcc actcgccacg gcgaagtttt tggcgaaacc cccgcttcac attttcaacg
     4321 agattatccg agaaatactt actctgcccg aacgccactt gcaacatgaa tttcccttgc
     4381 ggagttggct caaaccaaaa ttgcggaaag cgcaaagaag caattctgtt gatgtccaca
     4441 aggtaaataa ttttaccgcc atcaacagaa tttcgggcga gacgatcc
//
LOCUS       DATDSU010000099         5325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_261641, whole genome shotgun sequence.
ACCESSION   DATDSU010000099 DATDSU010000000
VERSION     DATDSU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261641"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..1704
                     /gene="ahcY"
                     /locus_tag="VI981_03300"
     CDS             445..1704
                     /gene="ahcY"
                     /locus_tag="VI981_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY4479356.1"
                     /translation="MKYDVKNIDLAKRGLERIRWAERDMPVLASIKKRFKKEKPLKNQ
                     TIAACLHVTSETATLMSVLKEGGANVFLCASNPLSTQDEVAASLVEDYKIPVFAVKGE
                     DTKRYFSHINAVLDQKPTITMDDGADLVSELHKNRVNQISKIICGTEETTTGVIRLKS
                     LAAAKKLRFPILAVNEAMTKHFFDNRYGTGQSTMDGIIRATNRLIAGSNFVVAGYGWC
                     GRGLASRARGMGANVIVTEIDPVKALEAVMDGFRVMPITEAARHADFICTVTGNRAVV
                     GAKEFAVMKDGCIVSNSGHFDVEIDLKNLRKIATSHMSVRDFVEEFTLKDGRHIYVLG
                     EGRLINLASADGHPASVMDMSFANQALGVEYMLHNYLSLEPKVYVIPEAVDRNIASLK
                     LAALQVSIDSLTPEQKKYLSSWDEGTS"
     gene            1719..2678
                     /locus_tag="VI981_03305"
     CDS             1719..2678
                     /locus_tag="VI981_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY4479357.1"
                     /translation="MKKRALVVGSVAFDVIFDIHGKLQDEILIENGKLGRQNLMFTAK
                     ERRQFFGGTGANISYGLGLLHAKPLLFSVAGNDFSGQFSKHLEKSGVTLRIVEEKGWT
                     ATFYGMSDTLNQQIGVYQPNAYKNIDSVSLSQSLSASDFKEIGVAIFSAGTGKSIHKH
                     IKELRQKKGSDAVIIFDPGQVISVFYNKKLLEETAKLADIFIGNEIEFKQLETILGYS
                     HSKLLHKGLRAVIKTQGERGSTIYEKNRTTHVKSVEPSKTIETTGAGDAYRAGLIYGI
                     LKKLDLESACRIGAYLGSKCVETVGGQAYKINLREIQKLFLRN"
     gene            complement(2863..>5325)
                     /locus_tag="VI981_03310"
     CDS             complement(2863..>5325)
                     /locus_tag="VI981_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY4479358.1"
                     /translation="DCTESYVDNTVVFIAAVPDSGSTFTGWTGACTGAGTCSVVMNSA
                     KLVGATFTGGNTTGNLSLTVSTLGTGIVTGTGISCGVDCTESFPANTAVTLTAVPSGT
                     ASFTGWSGACSGVGSCLVTLNSNKSVVASFSDAQFGLQPLTVLKQGGGTGTVTGTRII
                     CGADCTDSYANNTLVSLTAVPDFGFTFGGWTGDCSDSTICAIRMNGSKTVIATFLAPL
                     EVMPAPPLGLTAFTSASCQSVDVSWQPVAGATSYSLYESGTSNRLYSGPATTFTQGDL
                     IPNTGHSYTVTATLGGVTSRHSGQSPAGIASGSCATQASLKVMLVDASLDPLPNVTWI
                     VDAGIPQLHGAMVTLDSGTHEVRAKRRSGYILGVATCSYYGTSECFLNSPSEYATTNN
                     GLNCSGVYCTATVDVPAGGTLKVAFKYVKAPGNITANPVTDPPPCGGKASLSWTAVNG
                     ATGYNVYRSNDATGPFVQINAFPVLFPMYLDSGLTPLSTHYYSVSTLAGPIETPLTKP
                     SLGCSSELDVMLVLDRSGSIQQSDGVEALQTAAKNLISTLNPTADGMHMGLATFSGQA
                     TLNVPLGANASAMVNVIDNLSFGSGTQIDDGLRTAINELAGPNNRPDISDVIILITDG
                     EPTSDDCQPGVVPTLDEDHCGRLNALVEASRARDAGIDLFIVGVALDGSGRTFLPKLA
                     PDANHYVDAQFGTLDDELKSLTSCLPQSEILSVMAPASAVCPIVLDSIVCSSRPTPGY
                     TIQPIVWSTLALPVRDYTYEWHFNGLGPGLVEGAAPISTTTKYTSAGTKTTRVEVFFN
                     GERLGEASCNVEIFRLPGYQEG"
BASE COUNT         1523 a         1226 c         1241 g         1335 t
ORIGIN      
        1 tctctactcc ttgttggcct atgtgttggg ctgacgaaat tgttttatga aaactgactt
       61 ttgcgagaac tgcggacgac tgcggttgtt tgagatggat taaaaaagat gaggtgagag
      121 agttttgtaa ttcaaagtat attaccctgc gtcttgccgc aggggccata tttcaagata
      181 gttgaatctt ctcactcaaa caacaattga agcaaaatat tatatacgga cgatcgtttc
      241 tatataatat ttgtgatata cagtcgtgtc tgccaaaacg tgggtagtgg aagtaccgaa
      301 tatagtgtgt tggtaaattt ttaagattct ttggcggtaa gacgaatttt tgatctacac
      361 ttattcaggt ttcattacta ccaagttgaa attgcaccgg cccaaaaagt aagctagtat
      421 tgtcaaataa tttagagtaa tcgaatgaaa tacgacgtaa aaaatattga tctcgcaaag
      481 cgcggcctcg agagaatccg gtgggcagaa cgagatatgc cggtacttgc ctctattaag
      541 aaacgcttta aaaaagaaaa gcctctcaaa aatcaaacga ttgccgcgtg tctgcatgtc
      601 acctccgaaa ctgccacact tatgagtgta ctcaaagaag gcggcgccaa tgtttttctg
      661 tgcgcttcca accccctttc tacacaggac gaggtagcgg cttcactagt tgaggactat
      721 aaaattcctg tctttgccgt gaaaggtgaa gatacaaaaa ggtatttctc tcatataaac
      781 gcagttctcg atcagaaacc tacaatcacc atggatgacg gagcagattt agtttcggag
      841 cttcacaaaa atcgtgtcaa tcagatttca aaaatcatat gcggaacgga ggaaacaacg
      901 acaggagtca tccgcttaaa atcattggcg gccgccaaga aattgcgttt tccaattcta
      961 gctgttaacg aggcaatgac taagcatttt tttgataatc gctatggcac cggacagagc
     1021 acaatggacg gaattatccg tgcgactaat cgtctcattg ccggatcaaa ctttgtcgtt
     1081 gccggttacg gttggtgcgg acgaggcctt gcgtcgcgtg cgcgcggaat gggagcaaat
     1141 gttatcgtca ctgaaattga ccccgtaaaa gcactcgaag ccgtcatgga tggtttccga
     1201 gtcatgccaa taactgaagc cgcacggcac gccgatttta tttgtacagt caccggcaat
     1261 cgcgcagttg taggagcgaa agaatttgct gtaatgaaag acggttgtat cgtctcgaac
     1321 tccgggcact tcgacgttga aatagattta aaaaatttac gaaagatcgc gacatcacat
     1381 atgtccgtcc gagatttcgt agaagagttt actttaaaag atggacgcca catttacgtc
     1441 ttaggcgagg gtcggctcat aaatctcgct tccgccgacg gccaccctgc gtctgttatg
     1501 gacatgagtt ttgccaatca ggcgctcggc gtggaataca tgctacataa ctatctatca
     1561 ctcgaaccga aagtttacgt cattcccgaa gccgttgatc gaaatatcgc ctccctcaaa
     1621 ttggcggctc tacaagtttc tattgattct cttacgccag aacagaagaa atatttaagc
     1681 agttgggacg aaggaacgag ttaagtaaag ccccgtctat gaaaaaaaga gctcttgttg
     1741 tcggctcggt cgcttttgac gtcatttttg atatccacgg aaaactccag gatgagattt
     1801 taattgaaaa tgggaaactg ggccgacaaa acttaatgtt caccgcgaaa gaacgcaggc
     1861 agttttttgg cggcacggga gccaatattt cttatgggct aggactactc cacgcaaaac
     1921 cactgctttt ctccgttgct ggaaacgact ttagcggtca atttagtaaa cacctcgaaa
     1981 aatcaggcgt tacacttcgt atcgtggaag agaaaggctg gacggcaact ttttacggaa
     2041 tgtcagacac tctaaaccag cagatcggcg tgtatcagcc aaatgcttac aaaaacattg
     2101 attcagtttc actttctcaa tcgctatcgg ccagcgactt caaagagatt ggcgttgcaa
     2161 ttttttcagc cggaacaggc aaaagtatcc acaaacacat aaaagagctg cggcaaaaga
     2221 aaggttctga cgcggtgata atttttgatc ccggacaggt gatttcagtt ttttataaca
     2281 agaaactctt ggaagaaact gccaaattgg cggatatttt tatcggaaat gagatcgaat
     2341 tcaaacaact cgaaacaatt ttagggtaca gccacagcaa actgcttcat aagggcttga
     2401 gagcagtcat caaaacccag ggagaaagag gcagtacaat ttacgaaaaa aatcgcacca
     2461 cacatgttaa atccgtagaa ccttcaaaaa caattgagac aaccggggcc ggagacgcct
     2521 atcgagctgg tcttatatac ggaattctaa aaaaacttga cttagaatcc gcctgtcgga
     2581 ttggagcgta tctcggctca aagtgcgtcg aaaccgtagg aggtcaggcc tataagatta
     2641 atcttcgtga aatccaaaaa ctatttctta gaaattagtt cataatttca ctggagcgag
     2701 aattgtcttt tacaaaacat tgcgcaggac aaatcgcttt tttcctttca actaagcgag
     2761 cacattcctt tttaaatgcc ttggaaggcg cgacggtgcc ggacagagaa aatctttacc
     2821 aaaagatttt cgtgcattta aaaaggaatg agcgagcgcc ctttatcctt cctgataacc
     2881 aggtagtctg aagatttcaa cattacagct cgcctctcca agacgctcgc cgttaaagaa
     2941 gacttccact cgcgtcgttt tcgttcctgc cgaagtatat tttgttgtgg ttgaaatagg
     3001 agccgctccc tctaccaaac ccggtccaag cccattaaag tgccactcgt atgtataatc
     3061 gcgcacggga agtgcaagtg tgctccagac gatcggctga atcgtgtatc ctggagtcgg
     3121 ccgcgaactg cacacaatac tatcaagaac aatcggacag acggcgctag ccggagccat
     3181 tacggaaagg atttcagatt gtggcagaca gctggtgagc gacttcaatt catcatcaag
     3241 tgtgccaaac tgcgcatcaa cgtaatgatt cgcatctgga gcaagtttcg gaaggaaagt
     3301 tcgaccgctt ccatcaagcg ctacaccgac gataaacaga tctataccgg catcacgggc
     3361 acgcgatgcc tctaccaatg cattgagacg gccacaatga tcttcgtcca gagtcggtac
     3421 aactccgggc tggcaatcgt cgctcgtcgg ttcaccgtca gtgattaaaa tgatgacatc
     3481 cgaaatatcg ggacggttat ttggtccggc gagttcattt atcgccgtcc ttagtccatc
     3541 gtcaatttga gttccgcttc caaaactaag attgtctatg acgttaacca ttgccgaggc
     3601 atttgcacca agcggaacat tgagcgttgc ttgcccagaa aacgtggcaa gtcccatatg
     3661 cataccatct gccgtcgggt tgagcgtgct aataagattt ttcgcagccg tttgaagtgc
     3721 ctcgacacca tcagactgtt ggatactccc cgaccggtca agtacgagca tgacgtcgag
     3781 ttccgaacta caaccaagag agggttttgt gagcggagtt tctatggggc cagcgagggt
     3841 agagacagaa taataatgtg tggaaagcgg tgtgaggcca ctgtctaaat acatcgggaa
     3901 aagaacagga aacgcattga tttgtacaaa cggcccggta gcatcgtttg aacgataaac
     3961 attgtatccg gtagccccgt ttaccgctgt ccacgagagc gatgcttttc cgccgcacgg
     4021 cggaggatca gtgacagggt tggcagttat atttccaggc gctttcacat atttgaaagc
     4081 aactttcagt gttccgcctg ctgggacatc aaccgttgca gtgcagtaga cgcctgaaca
     4141 attcaatcca ttatttgttg ttgcgtattc agacggagaa ttcagaaaac attcggaagt
     4201 accataatat gaacatgtcg ccacaccgag tatgtagccc gaacgacgct tggcgcgaac
     4261 ttcatgagtt cctgaatcga gagtaaccat agcaccgtgc aattgtggga ttcccgcatc
     4321 aacaatccaa gttacgttag gaagcggatc aagacttgca tcaacaagca ttactttcaa
     4381 tgaagcctgg gttgcgcagg agcccgaagc gattccagcc ggtgactggc ctgaatgcct
     4441 cgacgtcact ccaccgagag ttgccgtcac tgtatatgag tgtccagtat ttggaataag
     4501 atcgccttga gtaaatgttg ttgctggacc actatacaat cgattcgatg tgcctgattc
     4561 ataaagagaa taacttgtcg ctccagcgac tggctgccaa ctgacgtcaa cagactgaca
     4621 acttgctgaa gtaaatgcgg ttagaccaag cggcggcgcc ggcataactt cgagcggagc
     4681 aagaaatgtg gcaataaccg ttttcgaccc attcattcta attgcgcata tcgtactgtc
     4741 ggaacaatct ccagtccagc cgccaaaagt aaaaccaaag tcgggtacag ctgttagtga
     4801 cacgagcgtg ttgtttgcat acgaatccgt acaatcggcg ccacaaatga ttctggtgcc
     4861 ggtcactgtg ccggttccgc cgccttgttt taagacagtc aggggttgaa gaccaaattg
     4921 agcatcagaa aatgaagcga ctactgattt gtttgagttt aaagtgacaa gacacgatcc
     4981 aactccgctg cacgctccgc tccagccggt aaaactggcc gtaccagatg gcaccgctgt
     5041 caatgtaacg gcagtattgg ctggaaaaga ttcagtacag tcaacgccgc aggaaattcc
     5101 cgtacccgtt actattccag taccgagcgt agaaacagtg agagataaat tacccgtcgt
     5161 atttccgccg gtaaatgttg ccccgaccaa cttcgcgcta ttcatgacca ctgaacacgt
     5221 ccctgctccg gtgcatgcac cagtccaacc tgtaaatgtc gagccactgt ctggcactgc
     5281 ggcaataaag acaacagtgt tatcaacata tgattctgtg cagtc
//
LOCUS       DATDSU010000100        16456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1764951, whole genome shotgun sequence.
ACCESSION   DATDSU010000100 DATDSU010000000
VERSION     DATDSU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16456
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764951"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VI981_03315"
     CDS             <1..762
                     /locus_tag="VI981_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479359.1"
                     /translation="PADTLMGKWGKFVGINKSLSEGVPVLAREDAYVYGEPNGDFLAM
                     VRKETKGRITEGPSIAGNETWWRVFFESEEDGWVREIQIERDLTGAGLKAFGSSLWYF
                     IRFFGTFLSLILLFGIVMVTWRFHGLIEPHQPYAEEVTAEPEAVPKEENKKWLRVLDH
                     TESLNPNDWRLAILEADIMLDDMLEIMGYRGPTLGDKLKTIEPSDFTNLDKAWEAHRT
                     RNLIAHEGADFQISQREARRIINLYKDVFKEFRYI"
     gene            1021..1093
                     /locus_tag="VI981_03320"
     tRNA            1021..1093
                     /locus_tag="VI981_03320"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:Met,seq:cat)
     gene            1224..1577
                     /locus_tag="VI981_03325"
     CDS             1224..1577
                     /locus_tag="VI981_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479360.1"
                     /translation="MRKYILFGLSQERNHMRLTMMAALLLLPLVVSANVPDYKPIVMQ
                     GRVHKEKAPMQQTDPKQSRASVTNDAIVVSVPLRKRAVQKKTEWFPEANSPSSRLDRP
                     KWPERRGLIIFERRF"
     gene            1701..2384
                     /locus_tag="VI981_03330"
     CDS             1701..2384
                     /locus_tag="VI981_03330"
                     /inference="COORDINATES: protein motif:HMM:NF025881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY4479361.1"
                     /translation="MYRSVNKNFFKKWTPKMAYVLGYFMADGYVTQNKRGANYFCFQI
                     CDLDILEKIKKELGSDHKISLKQKYKDEHSIQYRLQIGSKEICEDLSKLGVIAGKTNN
                     MKVPVVPRKYLHHFVRGYFDGDGNIWMGRIHKHRKNPTITIATMFTSCSRHFLRKLHE
                     KLRAAGIHGGSIYKSRENYSRLQFSSGPSLKLYDFMYNQCVNKSGLFLERKKSVFEKY
                     IQQKIAAVV"
     gene            2370..2444
                     /locus_tag="VI981_03335"
     tRNA            2370..2444
                     /locus_tag="VI981_03335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2405..2407,aa:Val,seq:gac)
     gene            2538..3125
                     /locus_tag="VI981_03340"
     CDS             2538..3125
                     /locus_tag="VI981_03340"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048044041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY4479362.1"
                     /translation="MRKLILASGSPRRKELLEKAGLQFEIVKSDYEEDMTLDLPPHEL
                     VEHLSLGKALDVAKKFNDAVVISADTIVVLDGEILGKPHTPENATEMLKKLNGKKHSV
                     FTGFTIIDIENNKTVTRSVETFVFFKNNAEAEIDAYVETGEPLDKAGAYAIQGLGGRL
                     IERIEGSLSNVIGLPVEELMGKLKELGIEDWKEAH"
     gene            3402..3680
                     /locus_tag="VI981_03345"
     CDS             3402..3680
                     /locus_tag="VI981_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479363.1"
                     /translation="MEEEYKKILKREGFNHVYEWHDKANQKYEEHAHKGKVSIFITGG
                     SITFNLDGKEYMFQKGERFNVPIGRPHIAIIGPSGCSYVIGEMVKGDT"
     gene            3743..5827
                     /locus_tag="VI981_03350"
     CDS             3743..5827
                     /locus_tag="VI981_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479364.1"
                     /translation="MDPNANEASQFTPQGISDPQPAVPPPVPPSETMPLTPNLSPCGH
                     TRGKLAVLIIISLIVVVALSGGGYYVVQNGVFKGAPYKEESIFSDSLKRASEIRQATY
                     SLKTSLSVVPRESGAVPFTIDNSNSPQLRAQYERDSKRANDISAILGALHYSYGDDGY
                     IPGVAGFGAQGKPASKPYPETLEALRSDLTKRNSYQANFLGTDPVTKNAYEYRLTENG
                     KNFELTAHFETTGATRSIKKSYSYVATTTLVNGQTVTFTKKSSTYLYVPSVPPKPFLV
                     MLGEQMRSMPPDVNMNGEVKATADFATDGIPDWDFNMDFSGSLGDLTYKFNADAKKKA
                     KDYFFRINNMPSIFFSALSSIKGQWIRVTSDSTSPKSEKDPYSYYYNEFEDLARELPK
                     LEESYKKGRGQFVDMLVKLAKIADEEKLTSIKERVGKEKVNGETLYRYKLELNEAAIV
                     SFYRRAAAELATGKNRAWYQDVVEDQGFLEYLESKEFKDLFAYYNQNTFFTLWVNKEG
                     VPARAEYRFRIVPPDTAIALRDKQVNLVFTMSLSNVNEPVTIEVPSSARSIRELMDEF
                     KKNQYEYSPEATLKSEDASLKASLSGIRASAEIVYDSNSGYEKKAFPLGPCSKKEGTL
                     FADSNVYKSLEMATANNVSNAICISVLKAGKVESYAVSISLPSSPDYSWCIDSSGNSM
                     QIAGSISKAQCK"
     gene            complement(5984..6277)
                     /locus_tag="VI981_03355"
     CDS             complement(5984..6277)
                     /locus_tag="VI981_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479365.1"
                     /translation="MLHHPGKRDELSYGVFNAFRDIIEINGERLSVDHAHNKAVWWAI
                     LEAVYASPFVVTRPGLSGIGHLFEIITHLRVERRADEMIYHVSVGNHPSCTVL"
     gene            6514..7203
                     /locus_tag="VI981_03360"
     CDS             6514..7203
                     /locus_tag="VI981_03360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4479366.1"
                     /translation="MSLHKKFWNKEYKNPKHFSLSDEASEDLEKFTRFLMRESGKKNL
                     NVTTNVLDMGCGNGRNLIYLSKTFGIHGTGIDISEEALHRARKMAETLPLKFEIGSVS
                     DPLAAADASVDIALDMMVSHFLTLHERTNLREEIVRVLKPDGWLFWKGPLLEEDQNAL
                     QMLNKYPGSEKNTYIHPTLKVQEYVWTEEVFEAFYEKEFEIIKTEKSYLHTLNGKAFK
                     RRTFTAYLRRK"
     gene            7254..7709
                     /locus_tag="VI981_03365"
     CDS             7254..7709
                     /locus_tag="VI981_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEY4479367.1"
                     /translation="MQQNKAIKILSWIVVLGAVFFLGFSSGHRGGTEAPVETQTEQKV
                     SAVSVMVDFGNGVIKTFNDVSLSVNASLFDVLKQLETENKIKLTVKDFGGDLGFFVEE
                     IDGIGKDPKGEKWWQFWVNNKYSDKGMSSNVMTKGDVVELKFIKGQQSQ"
     gene            7706..8248
                     /locus_tag="VI981_03370"
     CDS             7706..8248
                     /locus_tag="VI981_03370"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEY4479368.1"
                     /translation="MRKNATLKIGIAILLVVFGVLSRWLPHLGNFTPVFAITLFSAVY
                     LGRAYHIVVPISLMFMSDIGIGFYEWPIMISVYGSFILGGFLGELIKKSHLAVTVAAS
                     SFLGSLVFFLATNFAVWKWSPMYEQTFRGLMQSYEMGLPFFRNTLLGDLFYVALLFGA
                     YELVLYVVRKRREALSFAKS"
     gene            8339..8411
                     /locus_tag="VI981_03375"
     tRNA            8339..8411
                     /locus_tag="VI981_03375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8372..8374,aa:Thr,seq:cgt)
     gene            8749..8824
                     /locus_tag="VI981_03380"
     tRNA            8749..8824
                     /locus_tag="VI981_03380"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8782..8784,aa:Thr,seq:ggt)
     gene            8844..9125
                     /locus_tag="VI981_03385"
     CDS             8844..9125
                     /locus_tag="VI981_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479369.1"
                     /translation="MSIESGENPEKIIELKPEEYKTFARPFIIGSEHRDPEVYYRIPE
                     DVPFQNWRRDNGLEYNTGKKKVRLNGEEYYFNTDEKSYGALSFVEEPST"
     gene            9127..9810
                     /locus_tag="VI981_03390"
     CDS             9127..9810
                     /locus_tag="VI981_03390"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY4479370.1"
                     /translation="MEIPKSLESFAEPPTWKDLIKNLIFIAVAVGVAYFVTVRIGIDD
                     IRASVESAGIFAPLIIVFLKATTIVVVPLGGTPLYPIAGALYGFWPALGITLMGDAIG
                     SAIAFYLSRFFGKKILHFFMARNHMSMVEKLVEKASDTKVFLKARIFFTGFPELFAYA
                     AGLTKIRMPVFLVIHVGIHAIPASLLVVFGNLLVAGNMKTILLVGLVSTLLAFGGIVW
                     FHLNLTKGN"
     gene            10089..10964
                     /locus_tag="VI981_03395"
     CDS             10089..10964
                     /locus_tag="VI981_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEY4479371.1"
                     /translation="MTDKQKRIREIEEQMGNADFWADKTKSQAYIRELNELKAEKDGQ
                     NKYDRGNAIVTIFSGAGGDDAEDFSAILFRMYRKYIDKKGWSVVLLHSHENDHGGFRN
                     VSFEIEGKNVYSTLKHESGVHRLVRISPFNAKKMRHTSFSMVEVVPKLDKTDTNIQIP
                     DNELEVTVAKAGGPGGQNVNKRETAVRMVHVPTKLSVHVATERSLLQNKEKALEILKG
                     KLYKMREEERKAKEAGLSISKTTSIEWGNQIRSYVMHPYKMVKDHRTNLETSQIDKVL
                     EGHIDEFIEAEKSLN"
     gene            11207..11887
                     /gene="ftsE"
                     /locus_tag="VI981_03400"
     CDS             11207..11887
                     /gene="ftsE"
                     /locus_tag="VI981_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY4479372.1"
                     /translation="MIYFDKVSKIYNANSIALKDVTVTIEHKEFVSIVGHSGAGKTTL
                     LKMILAEERPTKGVVYFDSQNIHKLRPKMMNTIRRKIGTIFQDFKLLPNKTAFENIAF
                     AMEVAGRPDEEISADVPHVLELVGLGNKMESFPGELSGGEKQRVAIARAIVNHPDIIM
                     ADEPTGNLDPVNAFEIIHILKKIHDLGTTVILTTHNKGVVDSVGKRVITMEDGKITRD
                     DKHGKYSL"
     gene            12026..12910
                     /locus_tag="VI981_03405"
     CDS             12026..12910
                     /locus_tag="VI981_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY4479373.1"
                     /translation="MNFWRNSFVSLSSILIMSVTLLVIGCVIFTGVTLKASLAELEKK
                     VDINVYFMDTAPESDVLEIKKSLEALPEVLAVEYISREQAIADFKERHKDDQLTLQAL
                     EELGKNPLGATLNIKAKEPSQYAGIDKFLSGDSVIYKNGVRVIDETNFNDNKTAIERL
                     NKIMDSARKLGLVITLVLTLLSVLITFNTIRLTIFVSREEIQVMQLVGASYKFIRGPF
                     VVCGVLYGLVAGVITLIIFYPVTFWVRDATADFFGTINLFDYYLANFSQMFFIVIGAG
                     VLIGALSSYLAVRRYLRL"
     gene            13154..14848
                     /locus_tag="VI981_03410"
     CDS             13154..14848
                     /locus_tag="VI981_03410"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY4479374.1"
                     /translation="MKKQNHKYIFVIGGVMSGVGKGIATSSIGKILEAKGFNVNPIKV
                     DPYLNVDAGTMNPTEHGEVFVLNSGLETDQDMGNYERFMEKDLTPEDYMTSGMVYKSV
                     IDRERALGYGGKFVEAIPHITDEIKRRIVRAAHVNKSDISLIEIGGTVGDYQNILFIE
                     AARTLKIKNPHDVFFILVSYLPIPSKLGEMKTRPTQNAVHQLNSYGVQPDMIIARSHE
                     PLDHKRKEKISTWCSIPAQNVISAPDVDSVYDVPLNFEKDKISDIILKNLNLAPKKRG
                     DVLKDWRKFVAKIKNGTQDLHIAVVGKYFDTGDFFLSDAYISVIEAVKFSSYWQNTRP
                     RLTWINSKEFETGKLRIEMLMEFDGIIVPGGFGSSGIDGVIETISFARKNKIPYFGLC
                     YGMQLMVVEYARNVVGLKSAHTAEIDPGSKHLVIDIMPDQKKKIADGNYGGSMRLGSY
                     KCVLGKNTLAAAAYAEKKIKERHRHRYEVNPEYIERLEKAGLVFSGRSPDGTLMEIAE
                     LPASEHPFFLGTQFHPEFQARPLHPHKLFTAFIKAVIQEKKKKTKVPAYVEVTEKV"
     gene            14926..15198
                     /locus_tag="VI981_03415"
     CDS             14926..15198
                     /locus_tag="VI981_03415"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4479375.1"
                     /translation="MKKDIKKLTTSFKALANERRLLILKFLRKKGEASVSEIAREIKL
                     SIKSTSRHLSVLRAVDFVECEQRSLLVFYCVSRTLDREIKHLVTLF"
     gene            15383..>16456
                     /locus_tag="VI981_03420"
     CDS             15383..>16456
                     /locus_tag="VI981_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEY4479376.1"
                     /translation="MKTKSRSSKLKLEPLIPLDLKKCRTVSEIVGGMSHCAFGARMLG
                     EVCAMLFDWTNRNGKPYVIRDHLDLANEKLLGVMYQHGWFQGPYTSLEFIDHCRRAGF
                     KERKALVLGNYHEGTADELYRCANEAIFVNQHGFARPGQIQDGFFPNAVFIDPNLALP
                     IFFAVLKERKGFGKVSVPELFLRLKQHAGLAHDLVAGGETLLAMYRDKHCVRFLTLAG
                     AMTIAKMQLLICDMIDNCLVDHISSTGALMAHGLIEGIGDRHYKHNPENDDKLLAARG
                     INRVTDSLEPETNFDHLDDVMDGVLDGFTGTRDIAPSEIHARIGQYLREHHPTCRGVL
                     RSAYDKRVPVCVPAFVDSEIGNDV"
BASE COUNT         4791 a         3405 c         3834 g         4426 t
ORIGIN      
        1 cccgccgata cactcatggg taagtggggc aaattcgtgg gaattaataa gtctctgtct
       61 gaaggagtcc ctgtccttgc acgcgaggat gcgtatgtgt atggagaacc aaacggcgac
      121 tttttggcca tggtccgaaa agaaactaaa gggagaatta ctgaaggccc gtccattgct
      181 gggaacgaaa cttggtggcg cgtatttttt gaatcggaag aagacgggtg ggtacgcgaa
      241 atacaaatcg aacgagatct aactggtgcg ggcctcaaag ctttcgggtc gtcactctgg
      301 tacttcatca gattttttgg cacttttctt tcgctaatcc ttctcttcgg tattgtcatg
      361 gtgacgtgga gatttcacgg gctgattgag ccgcatcaac cgtatgccga agaggtgaca
      421 gcggagccgg aagctgttcc gaaagaagag aataaaaaat ggctgcgcgt acttgatcac
      481 actgaatccc tgaatccaaa tgactggcgg cttgccatcc tcgaagctga catcatgctc
      541 gacgatatgc ttgagatcat gggttatcgg ggtccaacgc ttggtgataa attaaaaacc
      601 atcgagccgt ccgattttac aaacctcgat aaggcctggg aggcgcacag gactagaaat
      661 ctgattgctc atgaaggcgc ggatttccaa atttctcaac gtgaagcgcg aagaatcatc
      721 aatctatata aggatgtttt taaagagttt cgatatatct aaacttctgg gttttgataa
      781 acgaaaaacg ccccatgaag gcgtttttcg ttagtttgtt gcgggggccc gaattgcacg
      841 ggcatctcaa ggttatgagc cttgcgaatt actgttactc taccccgctg tatgatactc
      901 gctgattata gccaaaaata taaatatatc aaacttaccc aacattactc caattagaaa
      961 tcttttgagg cattttacta attgtcttca tgaagcaggt atactatatt gatgtttatc
     1021 gtcggggtag ctcagttggt agagcacagg actcataagc ctgttgtcgc gagttcgatc
     1081 ctcgcccccg acacactaca ttatcagtac ctctcatttt aagaaataga aacgatcgtc
     1141 cggatattac taattatgga cgcggtattg tgcgactttt attatgccaa aggtgtgcta
     1201 ctattttcgg cggcttatgc tctatgcgaa aatacattct cttcgggtta agccaagaaa
     1261 ggaatcacat gcgactaacc atgatggcgg ctctactact gctgcctctc gttgtctcgg
     1321 cgaatgtgcc tgactacaag ccaatcgtga tgcagggacg ggttcataag gagaaagcac
     1381 caatgcagca aactgatccc aaacagtctc gcgcgtcggt aaccaacgat gcaattgtcg
     1441 tctccgtgcc gctccgcaag cgtgccgttc aaaagaagac cgaatggttc cctgaagcca
     1501 actcaccaag cagtcggctc gaccgtccta aatggccgga aaggagggga ctgattattt
     1561 tcgaacgacg attttaacgc gcaggcatcg cccgcgcttt tttgtacaaa aaatctcaaa
     1621 tgctcgtgcg atcaagttat aaaaatgggg acaattggtt agttatttag cctgtcacgt
     1681 gatatacttg aattgtgcca atgtatagat cagtaaacaa gaattttttc aaaaaatgga
     1741 caccaaaaat ggcctatgtt ctcgggtact tcatggcaga cggatacgtt acccaaaata
     1801 agagaggagc aaattatttt tgctttcaaa tatgtgattt agatatttta gaaaaaatta
     1861 aaaaggagct cggctctgat cataaaatta gtctgaaaca gaaatataag gatgaacatt
     1921 caatacagta tagattgcaa atcggtagca aggaaatatg cgaagaccta agtaagctag
     1981 gagttattgc gggaaaaacc aataatatga aggttcctgt cgtgcctcga aaatatcttc
     2041 accattttgt tcgaggttat tttgacggag acggaaatat ttggatggga cgtattcata
     2101 aacatcgtaa aaatcccact atcactatag cgacgatgtt tacgtcttgc tcgaggcatt
     2161 ttttgcggaa attgcacgaa aaacttaggg cagcaggaat acatggagga tctatctata
     2221 agtctaggga aaattactct agattacaat ttagctctgg tccttctttg aaattgtatg
     2281 attttatgta taatcaatgc gttaataaaa gtggtttatt tctcgaacgt aagaagagtg
     2341 tttttgagaa atatatacag caaaaaattg cggccgtggt gtagtctggt taacataatc
     2401 ccctgacacg ggatagatcg ccggttcaaa tccggtcggc cgcgcagacg aagtgaatca
     2461 catgcaattc acaaaaagta cacaggagat accacacttt ggtattgccg ctatttaaaa
     2521 aagctaagaa ttccattatg cgaaaattga tacttgcatc tggttcaccg cggcggaagg
     2581 aactgcttga aaaggcgggt ttgcaatttg aaatagtgaa aagcgactac gaggaggata
     2641 tgacgctcga ccttcctcct catgaacttg tagagcatct ttcacttggc aaagcccttg
     2701 atgtagcaaa aaaatttaat gatgcggttg ttatttcagc cgatacgatc gttgtattag
     2761 acggcgagat tttaggaaaa ccgcacacgc cagaaaatgc aacagaaatg cttaaaaaac
     2821 tgaatggtaa aaaacattca gtctttaccg gatttacaat tattgatata gaaaataata
     2881 aaactgtaac tcgaagcgtc gaaacttttg tattttttaa gaataatgcc gaagcggaaa
     2941 tagacgcgta tgttgaaacc ggtgagccgc ttgataaggc aggcgcatac gcaatacagg
     3001 gtcttggagg aagactcata gaacgtattg aagggagcct ctctaatgtt attggtcttc
     3061 ccgttgaaga attgatggga aaactcaagg aattgggaat tgaagactgg aaagaagcgc
     3121 actaaaacaa gtgccgtttt tgtggtatac tttctaaaat cgaaagttac tcacgatatt
     3181 ttcgtcataa tagctcttgg gcaaggcacc atatatttac tgttggcctt gcttatctat
     3241 ttttcacgat taattaactc atatatgtat ggatggggta gtagaaaatg gcattggata
     3301 acaaaatatt agaaaaatgc aaacagagat aaatatattt acaatatcga tttgattagc
     3361 acacgctctc caagtagggt gatgccattt ttcactaccc catggaagaa gaatataaaa
     3421 aaattctcaa gcgcgaaggc tttaatcacg tatatgagtg gcacgataag gccaatcaaa
     3481 aatacgaaga gcatgctcac aagggtaagg taagcatatt tatcaccggc ggttcaataa
     3541 cgtttaattt agacggaaaa gaatatatgt ttcaaaaagg tgagcgattc aatgttccta
     3601 tcggaagacc tcacattgcc atcatcgggc cttcgggatg cagctatgtc ataggagaaa
     3661 tggtaaaggg ggatacgtaa tttgtcccta gacgtcatct aggagtcgtt agataattcc
     3721 agcattaatt gttctaacga ctatggatcc aaacgcaaat gaagcaagtc agtttactcc
     3781 gcaaggcata agtgacccgc aacccgccgt gcctcctcct gtgcctcctt ccgaaacgat
     3841 gccgcttacg cccaatttgt ctccatgtgg tcatactcgc ggaaagttgg cagtcctaat
     3901 cattatttct cttattgtag ttgtcgcgct aagtggcggg ggctattatg ttgtccaaaa
     3961 tggcgtcttc aaaggagcgc catataaaga agaatcaatt ttttcggatt ctttaaagcg
     4021 cgcatcagaa atcagacagg caacctattc tcttaaaaca tcgctttccg tcgtcccgcg
     4081 agaatccggc gcagtgccgt ttacgattga taattcaaat tccccacaat tacgtgctca
     4141 atatgagcgc gactcgaaac gcgcaaatga tataagcgcc atacttgggg ctctgcacta
     4201 tagctatggc gacgatggat atattccggg agttgcaggt ttcggtgcac aggggaaacc
     4261 tgcgtcgaag ccatatccgg aaacgcttga ggcgttgaga agtgatctaa caaaaaggaa
     4321 ctcataccaa gccaattttt tgggtacgga tcctgtgacg aaaaatgcct acgaatatcg
     4381 tttaaccgaa aatggtaaaa attttgagtt aaccgctcat ttcgaaacta caggagctac
     4441 tagaagtata aaaaaatcgt attcgtacgt ggccacaacg actctggtaa atggacagac
     4501 tgttacgttt acaaagaaga gttccacata cctatacgta ccgagtgttc cccctaaacc
     4561 ttttcttgtc atgcttgggg aacagatgcg aagtatgccg ccagacgtaa atatgaatgg
     4621 agaagttaaa gctactgctg actttgccac tgacggaatt cctgattggg actttaatat
     4681 ggacttctcc ggttctttgg gtgatctcac ctacaaattc aatgctgacg ctaaaaagaa
     4741 agcaaaagat tattttttca gaatcaacaa tatgccaagt attttcttct cggcgctgtc
     4801 gtcgataaag ggacaatgga tacgagtgac aagcgattca acgtcgccga aatctgaaaa
     4861 agatccttat tcgtattact acaatgaatt cgaggacttg gcacgcgagc ttcctaaatt
     4921 ggaagaatca tataaaaagg gccgaggaca gtttgtggat atgcttgtta aactcgcaaa
     4981 gattgccgat gaggagaaat taacttcaat taaagaaagg gttggtaagg aaaaagttaa
     5041 cggtgaaacc ctctatcgct ataagcttga attaaacgag gctgccattg tttcttttta
     5101 cagacgtgcc gctgctgaat tggcaacggg caaaaatcgg gcgtggtatc aagatgtagt
     5161 ggaggatcaa ggttttcttg agtatctcga gagtaaggaa tttaaagatt tattcgcata
     5221 ttacaatcaa aacaccttct tcacactttg ggtaaacaaa gaaggtgttc cggcgagagc
     5281 ggaatatcgt tttagaattg tcccacccga cactgctata gcgcttagag acaaacaggt
     5341 gaatctcgta tttaccatgt cactctcgaa tgtaaacgaa ccagttacaa tcgaggttcc
     5401 ttcgagtgct cgttccatta gagagcttat ggatgaattt aaaaaaaatc agtatgaata
     5461 cagtccagaa gcgacgctga aatcagaaga cgcgtccctc aaggcttctt tgtcggggat
     5521 acgcgcttct gcggaaattg tatacgattc gaacagtggt tatgaaaaaa aagcttttcc
     5581 tctggggccg tgcagtaaaa aagagggcac gctcttcgct gactcaaatg tatacaaaag
     5641 tctcgagatg gctactgcaa ataatgtttc gaacgcgatc tgtatctctg tattaaaagc
     5701 gggcaaggtt gaatcatatg ctgtttcgat ctcgcttcca agttctccgg attatagctg
     5761 gtgtattgat agctcgggaa actcaatgca aatagcaggc tctatctcta aagcacagtg
     5821 taaatagggg ctaaggtagg aattttagga tttatatctt aagctctcaa aatacaataa
     5881 tgatgcgacc gcattattat tgtgcttata gacgagaaaa ctctcaagtg gccttatggc
     5941 gagagtacgg catattattg cgacgcgtta caaaaatgca ctgctagagt acagtgcatg
     6001 aggggtggtt accgacagaa acgtggtaaa tcatttcgtc cgcacgtctc tcaactctca
     6061 agtgcgtaat gatttcaaaa agatgaccga taccggataa cccggggcgc gtaaccacga
     6121 atgggcttgc gtagactgct tcgaggattg cccaccagac ggccttgttg tgagcatggt
     6181 cgaccgaaag acgttcaccg ttgatctcaa taatgtcacg gaaggcattg aaaacaccat
     6241 aagacaattc gtcgcgtttc ccgggatgat gaagcatata tttttctttt ggtatactaa
     6301 cataacatgc aaacaggtaa aaaattttgg aataaagtac gctcggacat ggtcttttaa
     6361 aaaacacaga taaaacgttg ctacgttttt cttctgctcg cgccgccgcg ctgcgcaagg
     6421 tattttaaaa gaatgtcctc gctctggcgt gagatgcacg tattaagcgg caccgatctt
     6481 gtattgtgct cggtacttgc ttattatgcc tagatgtctc tgcataaaaa attctggaat
     6541 aaggagtata aaaatccgaa gcatttctca ctatctgacg aagcttcgga agatttggaa
     6601 aaatttacgc gttttctcat gcgcgaatcg gggaaaaaaa atctcaacgt aaccacgaac
     6661 gtgctcgata tgggctgcgg caacggtcga aatctaatat atctctcaaa aacattcgga
     6721 attcacggga caggcataga tatttccgaa gaagcgcttc atcgggcgcg aaaaatggca
     6781 gaaactctgc cgctcaaatt tgaaattgga tcagtttccg acccattagc agcggccgat
     6841 gcttccgtcg atatcgcgct tgatatgatg gtcagtcatt ttttgacctt acacgaacgc
     6901 acgaatttgc gtgaagaaat agttcgagtt ctgaaaccag acggatggct attttggaag
     6961 ggaccgcttc ttgaggaaga tcaaaatgct ttgcaaatgc tcaataaata tcccggttct
     7021 gaaaaaaaca cctacataca tccaaccttg aaggttcagg aatatgtttg gactgaagag
     7081 gtttttgagg ccttttacga aaaggaattt gaaataatca aaaccgagaa atcctatcta
     7141 cataccctga atgggaaagc attcaagcgg cgcacgttca ctgcatattt gagaagaaaa
     7201 taattgtttt ttagcaaaag acaagtacaa tagtgagaca tttctaaacg catatgcaac
     7261 aaaataaagc tattaaaatt ttgagctgga tcgttgtgct aggtgccgtt ttcttcttag
     7321 gtttttcgag cggccacaga ggcggtaccg aggcgcctgt ggaaacacaa accgaacaaa
     7381 aagtttctgc tgtttcagta atggttgatt ttggaaacgg cgtaatcaaa acgttcaatg
     7441 atgtatcttt gtccgtgaat gcgtcgctct tcgacgtttt aaaacagctt gaaacagaaa
     7501 acaagatcaa gctgacggtc aaggattttg gaggagatct cggattcttt gttgaagaga
     7561 tcgacggcat tgggaaagat ccgaaagggg agaaatggtg gcagttttgg gtaaacaaca
     7621 aatatagcga caaaggcatg agttcaaatg tgatgacgaa aggtgatgtt gtcgagctga
     7681 aattcattaa aggtcagcag agccaatgag gaagaatgca actctaaaaa tcggcattgc
     7741 gattttgctc gtcgtgtttg gtgtactctc gcgttggttg ccgcaccttg gcaactttac
     7801 gcccgttttc gccataaccc ttttttccgc tgtctatctt gggcgggcct atcacattgt
     7861 cgttccgata tcactcatgt ttatgagtga tatcggcatc ggtttttacg aatggcccat
     7921 catgatttca gtctacggct cttttatttt aggcggtttt ttgggcgagt taatcaaaaa
     7981 atctcatttg gccgtgaccg tcgcggcgtc gagttttctc ggctcccttg tgtttttttt
     8041 ggcaacaaat ttcgccgtat ggaagtggag tccaatgtat gagcagacat ttagaggtct
     8101 catgcaaagt tatgagatgg gtttgccttt ctttcgaaat acattgttag gcgatctttt
     8161 ttacgttgcg cttctctttg gagcgtatga acttgtgctc tatgttgtcc gaaaacgcag
     8221 agaagctttg tcttttgcaa aaagctagga aataaaatac gatacgatca accaaggctg
     8281 ccaccctagc tcccagcctg aggctggtcc gcctaaggcg gaagttggtc ccgttagagc
     8341 cgaagtagct cagttggtag agcaatcgct tcgtaagcga tgggtcgcca gttcaattct
     8401 ggccttcggc tctaacggga tgaatgatgc accgttttcg cgcgccccgt agtaaatgcc
     8461 agtatgacaa gataatgtta attataagcc agcatttttc gaatgggtta ttaaattatt
     8521 taagcctacg actgtaagca tgttactact ggggtaaagt gggagcccgg agttcgattc
     8581 cccggggtgg cccacgaata atttgaaaat ctctaataca ctaactacat acgatcgtgt
     8641 gctattagtg tatttgggtc cgtgagaaat tatttgcttt ttcaagcgta tttattgtcg
     8701 atctcccaat tgaaaaactg tgactattac tttatgattc agcgatatgc cgatgtagct
     8761 cagcgagtag agcaactcca tggtaaggag taggtcgcca gtgcaaatct ggccatcggc
     8821 tccagttact atattaaaaa tttatgagta ttgaatcagg agaaaatcca gaaaaaataa
     8881 ttgaattgaa accggaagag tataaaacat tcgctcggcc atttattatc gggtctgaac
     8941 acagagatcc agaggtctat tatcgtatac ccgaagatgt accgtttcag aattggcgca
     9001 gagacaacgg actcgaatat aatacgggaa agaaaaaagt acgattgaac ggagaggagt
     9061 attattttaa taccgacgag aaatcctatg gggcgctctc tttcgttgaa gagccatcta
     9121 cgtaatatgg aaattccaaa aagccttgaa tcatttgccg agccgccgac atggaaggat
     9181 ttaattaaaa atcttatttt tattgccgtc gccgttggtg ttgcctactt tgtaaccgta
     9241 cgtattggca tcgatgacat acgcgcttcg gtggaatcgg cgggaatttt tgcgccgctt
     9301 atcattgttt ttttgaaagc gacgacgatc gttgtggtgc cgcttggcgg aacccctctc
     9361 tacccaatag caggagcgct ttatggtttt tggccggcac ttggcatcac gctcatgggc
     9421 gacgccatag gttcagcgat cgctttttat ctgtcgcgtt ttttcggcaa aaaaatcctt
     9481 cattttttta tggcgcgcaa ccatatgtct atggttgaga aactggtaga gaaggcaagt
     9541 gacacaaaag tttttttgaa ggccaggatt ttttttacgg gattccctga attgtttgcc
     9601 tatgccgcgg gactgaccaa aattcggatg ccggtatttc tagttattca tgtcggcatt
     9661 catgccatcc ccgcgtctct cctcgttgtg ttcggcaact tgctcgtcgc aggaaatatg
     9721 aaaactattc tattggttgg gcttgtaagt acactgcttg cgttcggggg gattgtttgg
     9781 ttccacctaa atctcaccaa aggaaattaa gggttttcaa accaaaactt gtgtttgtac
     9841 aaagcgagaa tataatcgaa taggagtatc tcgctttgaa tcttgaaccg ctggggctgt
     9901 tgggacgcac ctcctggctg gcgcacctta ctggcgacgc gatgcccctt cctatcggag
     9961 agcttgtttg attcttcgat gtaaaaaagc caacggcggt tcagaatttg ggggctctgt
    10021 ttgacgacaa gcccccttct gttttacaga cggaaccaaa aatggtctgt gttatactcc
    10081 tctttaccat gacagataag caaaaaagaa taagggaaat cgaagaacaa atgggaaatg
    10141 ccgacttttg ggctgataaa acaaaatcac aggcttatat cagggaattg aacgaactca
    10201 aggctgaaaa ggatggtcag aacaagtatg atcgcggaaa cgccatcgta accatttttt
    10261 ccggcgccgg cggcgacgat gccgaagatt tttcggccat tctttttcgc atgtatcgga
    10321 aatatatcga taaaaaaggc tggagcgtcg tacttttgca ttcgcacgaa aatgatcatg
    10381 gcggatttcg aaatgtttct tttgaaattg agggaaaaaa tgtttacagc acgctgaaac
    10441 atgagtctgg tgtgcatcgg ctggttagaa tatcgccctt taacgccaaa aaaatgcgcc
    10501 acacctcttt ttcaatggtg gaagttgtgc caaagctcga taaaaccgac acaaatattc
    10561 aaattcccga taatgaactc gaagtaactg tcgcaaaagc aggagggccg gggggacaaa
    10621 atgttaataa gcgcgagacg gcagtgcgca tggtgcatgt gccgaccaaa ctttccgtac
    10681 acgtggccac ggagcgttca ctgctgcaaa ataaagaaaa agcacttgaa attttgaaag
    10741 gcaagctcta caagatgcgc gaggaggagc gaaaagcaaa agaggctggg ctttctattt
    10801 ctaaaactac aagtatagaa tggggcaacc aaattcgctc ttacgtcatg catccataca
    10861 agatggtgaa ggatcaccgt actaacctag agacatcaca aatagacaaa gtcctcgaag
    10921 gccatattga tgaattcatt gaagcggaga aaagcttaaa ttaacgcctc ttgcggtttc
    10981 ggtgatctca agcaaagttt tatggcgccg caccaaattt gggggtgttt agggatgtgg
    11041 tatgattgct aatgcgtttt gaatatgatc ccagtagtaa agtcttaaaa gctgagaaag
    11101 tggtttaaaa ctgtgagcag gcatctcgtt tttctctgag ccatttgatt cggctgaact
    11161 tactacggga ttcatgattt tttaatcgct ttgctcaaga aaatctatga tctacttcga
    11221 caaagtatct aaaatctaca atgcaaattc cattgcgctc aaagacgtta cggtaaccat
    11281 tgaacacaaa gagtttgttt ctattgttgg ccattcagga gcgggcaaaa cgacgctttt
    11341 aaaaatgatt cttgcagaag aacggccgac gaaaggcgtg gtgtattttg attctcaaaa
    11401 catacacaag cttcggccga aaatgatgaa caccatacgc aggaaaatag gcactatttt
    11461 ccaggatttc aaacttcttc caaataaaac tgcttttgaa aatatagctt ttgccatgga
    11521 agttgctggc cgaccggatg aggaaatttc cgctgacgta ccgcatgttc ttgagcttgt
    11581 ggggcttgga aataaaatgg agagttttcc gggtgaactc tcgggcggag aaaaacagcg
    11641 tgtcgccata gcacgagcca ttgtcaatca ccctgatata attatggctg atgagccgac
    11701 ggggaacctc gatccagtta atgcatttga gattatccac attctcaaaa aaattcacga
    11761 tctcggcaca acggtgattt taaccaccca taacaaaggg gtagtcgact cggtcggtaa
    11821 acgcgtgatt accatggaag atgggaagat tacgcgagat gataagcatg gaaaatatag
    11881 tttgtagcga tgcgaatata ccaatgaatg cgaatgatac aaataaaata catcttgcat
    11941 tccattcgtt tcattcgtac cattcgttaa ttagtatcga tacatgtttc taaccaatgt
    12001 aaaaagaata ctgcgctccg gcctcatgaa tttttggcga aacagtttcg tctcgctctc
    12061 atcaattctc atcatgagtg tgacgcttct cgtcattggc tgtgttatct ttacgggcgt
    12121 tacgctaaaa gcctcgctcg ccgaacttga gaaaaaagtt gacatcaatg tgtactttat
    12181 ggatacggcg ccggaaagtg acgtgctcga aatcaaaaaa tcactcgagg cgcttcctga
    12241 agttttagca gttgagtata tttctcgaga acaagctatt gctgatttca aagagcgaca
    12301 taaagatgac caactgacgc ttcaggcact cgaggaactc ggcaagaatc cgctcggcgc
    12361 gacactcaac atcaaagcca aggagccttc gcagtacgcg ggaatcgaca aatttctctc
    12421 gggtgattct gttatttata aaaatggggt tcgtgtaatc gatgagacaa atttcaacga
    12481 taacaaaacg gcgattgagc gactgaataa aatcatggac tcggcgcgca agctcggact
    12541 cgtcattacc ctcgtgctaa cgttgctctc tgtcctgatc acgttcaata cgattagact
    12601 cacgattttt gtctcgcgcg aggaaattca ggttatgcag cttgtcggcg cgagctataa
    12661 atttatccga gggccgtttg tcgtctgcgg cgtgctctat gggctggtgg ccggtgtcat
    12721 aacgcttatc attttctatc cggtgacttt ctgggtccgc gacgctacgg cagatttctt
    12781 cggaaccata aatttatttg actattatct tgccaatttc agccagatgt tcttcattgt
    12841 cataggagcc ggtgtcctaa tcggcgccct ttcgagctat ttagcagtca gacggtatct
    12901 tcgtctataa tacgaaatac ggaatctcac ccgaattatg aatggcagat aggattacat
    12961 aggcccctgt ggtctttgcc caggggccta tattcatagc tgaaggcgca gggtttaaag
    13021 ttgggtctcg ttgcgcaatt tagggatgat agtgtgaagt ttaaaatagc actcaatata
    13081 cgataaaatg catggtatta ttttcatacg tcttgttatt cgtatttcgt actgattagt
    13141 tattcgtatt gtcgtgaaaa aacaaaatca taaatacatt tttgtaatcg gaggcgtcat
    13201 gtccggcgtt ggcaagggga ttgccacttc atccatcggt aaaattctcg aagcaaaagg
    13261 tttcaacgta aatccaatca aagttgatcc gtatttgaat gttgacgccg gtaccatgaa
    13321 tcccactgaa cacggggagg tttttgtttt gaattctggg ctcgaaactg accaggatat
    13381 gggaaactac gagcgcttca tggaaaaaga tctgacgccg gaagactata tgacgagcgg
    13441 catggtttat aaaagcgtta ttgatcggga gcgagcgctc ggctatgggg ggaaatttgt
    13501 cgaagcgatt ccgcatatta cggacgaaat aaaaagacgt atcgttcgcg ccgcccacgt
    13561 aaacaaatcg gatatttcgc tcatcgaaat cggtggaacc gtcggcgact atcagaacat
    13621 tctctttatc gaggccgcgc gaacgcttaa aataaaaaat ccacacgatg tattttttat
    13681 tttagtaagt tatcttccca ttccaagcaa actcggtgaa atgaaaaccc gtccgacaca
    13741 aaacgccgtg catcaactca attcctacgg tgttcagcca gatatgatca ttgcccgtag
    13801 ccacgagccg cttgaccaca agcgaaaaga gaaaatttcc acatggtgca gtattccggc
    13861 acagaatgtc atatcggctc ccgacgtaga cagtgtctac gatgtgccgc ttaattttga
    13921 gaaagataaa attagcgaca ttattttaaa aaatcttaat ctggcgccga aaaaacgggg
    13981 ggacgttctc aaagactgga gaaagtttgt cgcaaaaatt aaaaatggaa cgcaggattt
    14041 gcatattgcg gtggtaggaa aatatttcga tacgggcgac ttcttccttt cggatgcgta
    14101 catttcagtt atagaggccg tgaaattttc gtcctactgg caaaatactc gtccaagact
    14161 tacctggata aactcaaaag aatttgaaac aggtaaactc cgcatcgaaa tgctgatgga
    14221 atttgacggc attatcgtgc ccggaggctt tggcagttcc ggtatcgatg gggtcataga
    14281 gacaatcagt tttgcgcgga aaaacaaaat tccgtatttt ggcctctgct acggcatgca
    14341 gcttatggta gttgaatacg cgcgtaatgt ggtaggcctc aagagtgctc acacggccga
    14401 gattgatccc ggatcgaagc atttagtgat tgacatcatg ccggaccaaa aaaagaagat
    14461 tgccgatgga aactacggag gaagtatgag actcggttct tataaatgtg tgctcgggaa
    14521 aaatacactc gcggccgcgg cctatgcaga gaaaaaaatc aaagagcgcc accgccaccg
    14581 ctatgaagtc aatccagaat acatcgagcg gctcgaaaaa gcagggcttg tattttccgg
    14641 acgttccccg gacgggacgc tcatggaaat cgccgaactg cctgcatccg aacatccttt
    14701 ttttcttggt acgcaatttc atcccgaatt tcaggctcgg ccgcttcatc cgcacaagct
    14761 tttcacagct tttataaagg ctgttattca ggaaaaaaag aaaaaaacta aagttcccgc
    14821 gtatgttgaa gtcactgaaa aagtttgatc gggtaagtta cttcttacgg cactcttcta
    14881 ttctttaaga ctaattcgtg agaataagag acgatagaaa taaaaatgaa aaaagatata
    14941 aaaaaattga cgacttcttt taaggcgctc gccaatgaac gaagactgct cattttgaaa
    15001 tttttaagga aaaaaggaga agcttccgta tccgaaatag ctcgcgagat aaaattatcc
    15061 ataaaatcga catccagaca tctctctgtt ttaagggcag ttgattttgt tgaatgcgag
    15121 cagagaagtt tgctagtctt ttactgtgtg agtagaacac tagaccgaga aatcaaacat
    15181 cttgtaactc tcttttaaaa taaatacatg catgttgtta gtcccatgcc tttgtctatg
    15241 gacgtattcg tgagaattag aggtagtggg cagaaaatgg cagacacaga ataacgcagg
    15301 gttccgattg aaattatttg agttgacata gcttattttt cccgtacgat aggcggcagt
    15361 attccgaacg aaagaaaaaa acatgaaaac aaagtcccgt tcttccaaac tcaaactcga
    15421 accgctcatt ccgctcgact tgaaaaaatg tcggacagtg agcgagatcg tcggcggcat
    15481 gagccattgc gcgttcggtg cccgaatgct cggtgaagtc tgtgccatgt tattcgactg
    15541 gactaatcgc aacggcaaac cgtacgttat tcgagatcac cttgatctcg caaatgaaaa
    15601 gctgctcggc gtgatgtacc agcacggctg gtttcagggt ccatacacat cgcttgagtt
    15661 tatcgatcac tgccgccgcg ctggcttcaa agagcgaaaa gcgcttgtcc tcggaaatta
    15721 ccatgaggga actgcagacg aactctatcg ctgcgcaaac gaggccatct ttgtgaatca
    15781 gcacggtttt gcaaggcccg gacaaatcca agatggcttt tttccgaacg ccgtcttcat
    15841 cgacccgaac cttgcgcttc ctattttctt tgcggttctc aaggaacgca agggttttgg
    15901 gaaggttagc gtgcccgagc tttttctgcg gctgaaacag catgctggtc ttgctcacga
    15961 tctcgttgcc ggcggggaaa ccctgcttgc catgtatcgc gacaaacact gcgtccggtt
    16021 tctgacgctc gctggggcca tgaccatcgc caagatgcag cttctcatct gcgacatgat
    16081 cgacaactgc ttggttgatc acatttcaag tacgggagct ctgatggcgc acggtttgat
    16141 cgagggtatc ggcgacaggc attacaagca caatccggag aatgacgaca aattgctcgc
    16201 ggcaagaggc atcaaccgag tgaccgactc acttgagcct gaaaccaact tcgatcatct
    16261 tgacgacgtc atggacgggg tcttggacgg cttcaccgga actcgggaca ttgcaccgtc
    16321 cgaaattcac gctcggatcg gccagtacct gcgagagcat catcccactt gtcgcggcgt
    16381 actgcgctcg gcgtacgaca agcgagtgcc ggtctgcgtg ccggcttttg tggattcgga
    16441 gatcggcaac gatgtt
//
LOCUS       DATDSU010000101        13408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2263688, whole genome shotgun sequence.
ACCESSION   DATDSU010000101 DATDSU010000000
VERSION     DATDSU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263688"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..720
                     /locus_tag="VI981_03425"
     CDS             460..720
                     /locus_tag="VI981_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479377.1"
                     /translation="MNRQRGLVKLVIVIVIGLIVLGYFGLDLKEILQKPVVHNNLVYV
                     WNFIVIVWNRFIEAPATWIWREIFIEIIWYKLLLPLITKAKA"
     gene            complement(992..1612)
                     /locus_tag="VI981_03430"
     CDS             complement(992..1612)
                     /locus_tag="VI981_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY4479378.1"
                     /translation="MINRIYVIIAILVVVVGVFIYFSERDVVVTNYPGSGTSIIALGD
                     SLTAGVGATEGKDYVSRLNDKLGVLIINAGKSGDTTETALLRLYDDVLIKKPKLVIVM
                     LGGNDYLRKIPKEITFENLDTIIKRIHSSGSMVLLVGVQGGIIGDPYKREFEALAEKH
                     NTGYVSNILENIVGHNDRMSDGIHPNDLGYDKIAVRLEPAVKKLLK"
     gene            complement(1704..2921)
                     /locus_tag="VI981_03435"
     CDS             complement(1704..2921)
                     /locus_tag="VI981_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY4479379.1"
                     /translation="MDYKAEITDPNDMASQFARKIALESHNNCPAITVRPHDARNWRG
                     GTGVLWAKHVLEAMLQSHNSIGRSVMLEEMENDGAGGKPQFFTLHGSDRAFSGLGWEV
                     IVMCDHDFWRSGRFPAYICNQIDTKGLTPQNFRLFKAMMEGYGLALQRSGLANITGEI
                     AICPNQITAFCDQNSPSQLIVTWGAAVVGLSHCDRLIDGYLIEPGMDIVGLLENGYRC
                     NGGTWLTRLVLTKWGPSIDAIRNNPEAMDFIEMLTIPSVSYSSFLRVLIGWNPDASLR
                     PPAGRVYGIAHITGGGLAKFQEALPNGIGACLDEMPRPPEVLLQAQRYAAEKMTLSDE
                     AAYRTFHGGCGMMLVCHPDDTSNIIRLAHAHRHVACHVGKTCWSPTKEIRVTSRFRAK
                     LGTKIWPLLPDAK"
     gene            complement(3221..3688)
                     /locus_tag="VI981_03440"
     CDS             complement(3221..3688)
                     /locus_tag="VI981_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479380.1"
                     /translation="MTIIRLSAIVFSFATTFNNSTPATGVPFKSEDTTAIVEMLPAIP
                     EDKKSVSVEPHVKAYFADIPVLIKIAKCESTFRHADSEGEVVRGVVNKYDVGVMQINE
                     QYHKERAEKMGIDLHTLEGNLAYARFLYENHGTSPWSASELCWGKKAVELAKK"
     gene            complement(3901..4497)
                     /locus_tag="VI981_03445"
     CDS             complement(3901..4497)
                     /locus_tag="VI981_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY4479381.1"
                     /translation="MIVIDMESTGVDPIKHSLLSLGAVDFKNPKNQFYMECRAWEGAH
                     IDDEALLVNGFSKEQAFDQTKATDRELVEKFLTWVEAIPERTLAGQHPATDRDFLKYT
                     CFRHHINWPFAQRSIDLHTVAYYHMIKRGVSIPLKNGHSALNLDAILRYVGMETEPRP
                     HNGLTGAKVEAETFSRLFYGKSLLSDYERLPLPDFLKL"
     gene            4498..4665
                     /locus_tag="VI981_03450"
     CDS             4498..4665
                     /locus_tag="VI981_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479382.1"
                     /translation="MEIISTSEERVEAEGLHVVRTPFEYETNKVSALMSHLAIFNATG
                     TMWRENNRSST"
     gene            4687..6309
                     /locus_tag="VI981_03455"
     CDS             4687..6309
                     /locus_tag="VI981_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479383.1"
                     /translation="MKEIAVISAFNKNEKLLVFARGLKELGMEIWASRGSAAFLNNFD
                     VPAVDIASKVGEPILGHQVVTLDRGIYCKILGRPERQDELAELKKHGNEIIRVVRVDL
                     YPTEETIAKTGATLADVLDNMDIGGPTLLSAASKRRIAVCDEADHEPVLKELRRAKDA
                     GQPFDPEFREILGTKADFFVSWYRSLTANFRGPGRFLTIHATHLHDCAYGENHRQTPA
                     RVFTIKTGEQDPLRYEAFKHVGGSSPSYNNNLDLMGALRTVTELAATWKVNFGEVPYI
                     AVAVKHTNPCGVGVGAKPEIALKKAIDGNRKAIFGGCLMVNFPITNELALVIRDYNTA
                     GDSRRILDAVIASSFDDEAIKELERAKGKCRFFENGKLAELDEHSLYRGPLFAPLWGA
                     IETQPSVPFILDLKDTRIVRHGDKLTPQQERDMLIARVVCGVTTSNTISLAKDDMLKG
                     NGAGQQSRVIAARVALMVADDGGHNAGGAVGASDSFFPAPDGPEVLAKAGIGAMFTTS
                     GSRRDDETIAVFNWYRIPLVMLPDVVARGFRH"
     gene            6424..6678
                     /locus_tag="VI981_03460"
     CDS             6424..6678
                     /locus_tag="VI981_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479384.1"
                     /translation="MNKTVRPNNSEVPEEKPTAAALWTNMTVPERLGCLEYVPDTLFE
                     LQAVEERHDRMTELANRAFYTLSPSLRQSLERPDSVLFVH"
     gene            complement(6745..7683)
                     /locus_tag="VI981_03465"
     CDS             complement(6745..7683)
                     /locus_tag="VI981_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479385.1"
                     /translation="MKYRLFLFVFAFSALSTATAAENARQNLGGKDPSTNRSSNVTFR
                     LPLIRDTSSFWYRVEEIYERRTRIVFENSLRSVKSLKWQLRLQDHDDLGEKYERTAGS
                     AFVRSVSYSMRELVVETPLVVRSRSAIEERFGFLGRFLSDSFSNSLAAVDEDEIRVHG
                     ASFVVTDRTWLEGSYTNSSSGVADKSFLSRLGRLYDAGVLRPGANLFHENPYVFVTSR
                     IQLGGETWLIAHVRYRAMHLIGDHRVETLLSVPLHDGFYFDTGFSFEPWVKKDKERLS
                     FRVTKRWGRGARANAFFVGYEPREARILSGLAFDWP"
     gene            complement(7851..8228)
                     /locus_tag="VI981_03470"
     CDS             complement(7851..8228)
                     /locus_tag="VI981_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479386.1"
                     /translation="MSNLRKIIGHYGKDLAVVLIVALVAMASFQIGRLSMSGNSASPI
                     RLLQANLLGATNKNANAVEQLPQVPKLKAGGEVVASKNGTRYYFPWCKAASRINDENK
                     IYFASEEKAIEAGYTAAQNCAGL"
     gene            8402..8674
                     /gene="rpmA"
                     /locus_tag="VI981_03475"
     CDS             8402..8674
                     /gene="rpmA"
                     /locus_tag="VI981_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY4479387.1"
                     /translation="MATKKAGGSAKNISHQNPKYLGIKLYAGEKAFSGSIIVRQRGTR
                     ILPGENVGMGKDHTLYAEKEGTVSFGSKRKTNYDSSVSIKKIVHVK"
     gene            complement(8721..9161)
                     /gene="rplI"
                     /locus_tag="VI981_03480"
     CDS             complement(8721..9161)
                     /gene="rplI"
                     /locus_tag="VI981_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY4479388.1"
                     /translation="MKVILLKDVAKLGKKFDVKNVSDGHALNYLIPRGEVEHATDALI
                     KKIDETKVQEKAKIEKALHESAEKMQALSKQGIIMSGKANEKGHLFAAIHKAEIAAEL
                     KNKGILIDEDHIELEKPIKEIGDHTVGFRLGDQMTKIKIGVKKS"
     gene            complement(9348..10610)
                     /locus_tag="VI981_03485"
     CDS             complement(9348..10610)
                     /locus_tag="VI981_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY4479389.1"
                     /translation="MHFQTVWTKLRGFFTSNGVLIIIAIVVIGISFGSGVYTGLYHRP
                     EIEKVKDVTNKDNIVSAEADFEPFWKVWNVLNEKFVSTKKSKGDISAQDRVWGAAEGL
                     AASLGDPYTVFLPPVESEFFQTEISGNFEGIGAEVGMKDNILTIIAPLKDNPAYKAGI
                     LAGDKILKIDDTLTSDLSIDEAIRKMRGKKGTPVKLTILREKKKEPFEVTIIRDTIAI
                     PNVETEKRTDGVFVIGLRSFSAVSPDLFRNALKEFVLANTDKLVLDLRGNPGGYLEAA
                     VDMASWFLPEGKIIVRESFGPNKEETVYRSKKGYNIFTDKIKFVILVDVGSASASEIL
                     AGALQEHGVAKLVGTQTYGKGSVQELVPITKDTNLKVTIAQWLTPNGISISDGGVKPD
                     VEVKLSPEDIDAKNDLQKKKAIELVKSL"
     gene            11034..11948
                     /locus_tag="VI981_03490"
     CDS             11034..11948
                     /locus_tag="VI981_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY4479390.1"
                     /translation="MINRHIYKNLVWIDLESPTHEEIEEIAGQYKIGHLVTQELARPS
                     FKPKVETYPDYIYLILHFPVIRHSHSLDTDQEIDFILGKDFLITAHYDTVDPLHKFSR
                     IFEVNSILDKSNIGDHAGFIFFYMMRELYTSLANELEFVFETLKDIEARIFRGKEKEM
                     VFALSNVSRILLDFRQATIHHKDILDSFDEPARALLGNGFGPYVRATRDEYFKVTNTI
                     RSHGELLSELRETNNSLLNTKQNEIMRHLTMMAFVTFPLSLVVALLAIDTEYNPVLGL
                     PQDFWLIAGIIVFGMASIIVYFKWKKWL"
     gene            12027..>13408
                     /gene="cysS"
                     /locus_tag="VI981_03495"
     CDS             12027..>13408
                     /gene="cysS"
                     /locus_tag="VI981_03495"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY4479391.1"
                     /translation="MPLYLFNTLTKKKEEFIPLEPEKVRMYNCGPTVYNYAHIGNLRA
                     VLFVDLLRRALELSGYEVTQVMNITDVGHLSGDNDDGEDKMTNALRREGKAMTLQAMK
                     EIGEFYTQKFMDDLARLHVKTDNTKFPRASEHISEQIKLIQKLEQKELTYRTDDGVYF
                     DTSKISDYGKLGGVSREASVARIEMKSGKKSPRDFALWKFSDTGKDELGWQSLWGVGF
                     PGWHIECSAMAMKYLGETFDIHTGGVDHIPIHHNNEIAQSENATGKPFAHYWLHNAFL
                     NIRGEKIAKSVGNALFLHNLRSPLAYRYLILGATYRTQIDYTDESYEAAENAYKKFIK
                     EFSVLPKETGAVSESYKKDFDLALADDLNTPQALALAWQLLKDKSVRAQDKRATLLYF
                     DQIFGFNLEAESEISAKLEIPSKIEVLAKERDKARAAKDFKEADEIRLQIEKNGYMIE
                     DTPEGTRIVSA"
BASE COUNT         3636 a         3300 c         2934 g         3538 t
ORIGIN      
        1 aatcacgcga aaatcatccg aattgatata gcgacgtggt agagtgttat tttaatccta
       61 ttgaggcgat cggatcggaa gtgatcgatg tgttacgaac cgatgtgcga tatcggcaga
      121 gactctaagt ttctttcttc cgcgcctcgt tttccacact cgccaggttt ttttgcacac
      181 tacacattgc cgtctgcgcc cgccgaagcg gcgcacctgt cttcgacagc agggagattt
      241 ctcgtgtttt ttcttcatac acgagaaagt gtcctacgga aagaagaaat gtccctcatt
      301 tgaaggacat tctggatacg acatcaacca aatttttcag tcaaagtggc tatagcgtca
      361 aatcattgta catggatttt tagtggtaat tatgtaattg ggggatacat ctgaagatat
      421 tcacaacgaa ctttgacgta gaacggtata atactgccta tgaatcgtca gagaggactt
      481 gtgaagcttg tcattgtaat cgtgattgga ctcattgtcc tcggctattt tggccttgac
      541 ctaaaggaaa tactgcagaa acccgtggtc cataacaacc ttgtgtacgt ttggaatttt
      601 atagttattg tgtggaatcg gtttattgaa gccccagcaa cctggatctg gcgagaaatc
      661 ttcatcgaaa taatttggta taagctgctt ttgccactga tcaccaaagc caaggcttag
      721 tttctccgca taaattaggt acgccacggg acatggacat tagccgatcg acccagaaat
      781 gatctaaagc aggcgaatat tgtacgatgc ctgcgttgca caatattcgc tacattttac
      841 gacgcccgca gaaaataagt tctcaatgtt aaaaatactg atgcaatagg gtagacaccg
      901 ccacgagaga attgctaagc tgggcgtcgc aaaatgtata ttgtgcgaca tgcctatggt
      961 cagtctctcc acagagaaac ccctctcccc tctacttcaa aagctttttt actgccggtt
     1021 caagcctcac cgcaattttg tcatatccaa gatcattcgg atgaattccg tcgctcatgc
     1081 gatcgttatg gccaacaata ttttccaaaa tgtttgagac atatccggta ttatgttttt
     1141 cagccagtgc ctcgaattcg cgtttataag gatcacctat aatacctccc tgcaccccca
     1201 ccagtagcac catggaaccg gatgaatgga ttcgttttat aattgtatct aaattctcaa
     1261 aggtaatttc tttaggaatc tttcgtaaat aatcattccc accaagcata acgatgacaa
     1321 gctttggttt ttttatgagc acatcgtcat agagcctaag aagtgccgtt tctgtagtat
     1381 caccactttt gccggcattt ataatcaaaa ctcccagttt atcattgagc cgtgagacat
     1441 agtccttgcc ctccgtggcc ccgacaccgg cagtcaaact atctccaagt gcgatgatac
     1501 tcgtgccgct cccgggataa tttgtaacaa ccacatcacg ctcggaaaaa tatataaata
     1561 ctccgacaac tacgactaaa atagcaatga taacgtagat tcgattgatc ataatcgctt
     1621 ttcaatattc atattgtgag cttgagtata ctttaatttt tccgatatta aaaaagccag
     1681 actgattggt ctagcttgcg ttctcatttt gcgtcaggca gaagtggcca tattttagtc
     1741 ccgagcttcg cgcgaaaccg tgaggtaacc ctgatctctt tggtcggaga ccagcacgtc
     1801 ttccccacgt ggcacgcaac atgacgatga gcgtgtgcga gtctaatgat atttgaggtg
     1861 tcatcggggt gacaaacaag catcatgccg caaccgccat gaaaggtcct atatgctgct
     1921 tcgtctgaga gcgtcatttt ttcggccgca taccgttgtg cctgaaggag aacttccgga
     1981 gggcgtggca tctcgtctaa gcatgccccg ataccgttag gcagagcctc ttggaacttt
     2041 gcgagtccac cacctgtaat gtgcgctata ccgtacactc ggcccgctgg cggccgcagg
     2101 ctcgcatcag gattccagcc tatgagtacc cggagaaaac ttgaataact cacggatggt
     2161 atggtcaaca tctcaataaa atccatggcc tccggattgt tacgaattgc atcaatcgac
     2221 ggaccccatt tcgtgagaac cagccttgtt agccatgtcc ctccattaca gcggtaccca
     2281 ttttccagca gcccgacaat atccatgccg ggctcaatta gatacccatc aatcagccgg
     2341 tcacaatgcg agagtccaac gaccgcagct ccccaagtga cgataagctg gctcggagaa
     2401 ttctgatcac agaaggccgt aatttgattg gggcagatcg ctatttcccc ggtgatgttg
     2461 gcgagtccgg acctttggag tgcgagacca tacccttcca tcatcgcctt aaaaagacgg
     2521 aagttctggg gcgtcaggcc ttttgtgtcg atctggttgc aaatgtatgc aggaaatctt
     2581 cccgagcgcc agaagtcgtg atcacacatc actatgactt cccatccgag cccactgaaa
     2641 gctcgatcgg acccgtgtag tgtaaaaaat tgaggttttc cgccagctcc gtcgttctcc
     2701 atctcttcaa gcatgaccga acgaccaata gagttatggg actggagcat tgcttcaagc
     2761 acatgtttgg cccagagaac gcctgtcccg cctctccaat ttctcgcatc atgcggcctg
     2821 acagtaatag ctggacaatt attatgactc tcgagagcaa tttttctggc aaactgcgaa
     2881 gccatgtcgt ttgggtcggt gatttcagcc ttatagtcca taggaatttt ctttcggttg
     2941 tgcctgtcac acgtacgatg acccgtacac ggttgctgat taagagaaca gcatttctgc
     3001 gtaaagacta ctttagacca actacaaggt caacaaagca gagtacttta taaaagtctt
     3061 attttttttg agacttctgt taagaactcg tctatttaat tgccctagca tttaccagcg
     3121 atcgctggta aatgctaggg ttatgttcga tactcacagt ggggctacta gagagacacc
     3181 ccaactcaag aagttcaagc caaagttttc caatcagtct ttacttcttt gcaagttcga
     3241 cagctttttt gccccaacag agttctgacg ctgaccatgg tgatgtgcca tggttttcat
     3301 atagaaatcg ggcatatgcc aaattcccct ctagggtgtg caaatctatt cccatctttt
     3361 ctgcccgttc cttgtgatat tgctcgttga tttgcataac tccgacgtca tatttattga
     3421 cgacacctct taccacttca ccttcgctgt ctgcgtggcg aaaagtcgat tcgcacttgg
     3481 cgatttttat caggaccggg atatcggcaa aataggcctt cacgtgaggc tctacagaga
     3541 ctgatttttt gtcctccggt atagcaggga gcatttccac gatagcggtc gtgtcttcgc
     3601 ttttaaatgg aactccggta gctggagtgc tgttgttaaa tgttgtggca aaagaaaata
     3661 cgatggccga tagtcgaatt atagtcataa atttgtttag ttttggtcga tatttccatg
     3721 aatcaaatga aaaacccaga ccatctgagt tttaactgac ttatttgatt atgtccgcta
     3781 ttatagcaca cattgatctc ttgtcaagat ttcaccacgc attggagacg gagtcaaagt
     3841 caaaaatagg ctcttgtaga gccatttatt ccattgacgt atcgcccgcg acctgtccaa
     3901 ttagagcttc aagaaatcag gcaatggaag cctctcatag tcggaaagca gacttttccc
     3961 gtaaaagagt ctcgaaaatg tctctgcctc cactttcgct ccggttagac cattgtgagg
     4021 cctcggttca gtctccattc caacatatcg aagtatcgcg tcgaggttta gtgcagaatg
     4081 accattcttg agaggaatag acactcccct ttttatcatg tggtagtagg caacggtgtg
     4141 aagatcaatg gacctttgcg caaagggcca gttaatgtga tgccggaagc acgtatattt
     4201 caaaaagtcc ctgtctgtcg caggatgctg gcccgcaagt gttctttcgg ggatcgcttc
     4261 aacccacgtt aaaaatttct cgaccaattc cctatcagtc gcctttgtct ggtcaaacgc
     4321 ctgttccttt gaaaagccgt tcacaagaag tgcctcatca tctatatggg ctccctccca
     4381 agcacggcat tccatataaa attgattttt tgggttttta aaatcaacag ctccaagact
     4441 caagagcgag tgttttattg gatctactcc cgtcgattcc atatcaatga ctatcatatg
     4501 gaaattatat caacaagtga ggaacgagtg gaagccgaag gcttacacgt cgtccgaacg
     4561 ccgttcgaat atgagacgaa caaagtgagt gctcttatgt cacatcttgc aattttcaat
     4621 gcaacaggca ctatgtggag agaaaataat agatcttcta catgaagatc gcaaccaacc
     4681 ccccccatga aagaaatagc tgtcatctct gccttcaaca agaacgaaaa actgctggtc
     4741 tttgcccgag gtctcaaaga gctcggtatg gaaatctggg cctctcgcgg atcagcggca
     4801 ttcctaaaca actttgacgt gccggcggta gatattgctt caaaggtcgg cgaaccaatc
     4861 ctaggacacc aagtcgttac gttggatcga ggtatttatt gcaaaatcct cggacggccg
     4921 gaacgtcaag atgaattggc cgaactcaag aaacacggga acgagatcat cagagttgta
     4981 cgtgttgacc tctacccgac cgaggagaca attgctaaaa caggagcgac ccttgccgat
     5041 gttctcgaca acatggacat cggcggtcca actttgcttt ctgccgcctc gaagaggcgc
     5101 atcgccgtct gcgacgaggc ggatcacgag cctgttctca aggaacttcg tcgggcgaag
     5161 gatgcaggtc aacctttcga cccggaattt cgcgagattc tcggcacgaa agctgacttc
     5221 tttgtcagtt ggtaccgatc gcttacggcg aacttccgcg gtcccggaag gttcctgacg
     5281 attcacgcta cccacttgca tgactgcgcg tatggcgaga atcaccgcca aacgccggca
     5341 cgtgtcttta ccattaagac tggggagcaa gacccgcttc ggtacgaggc attcaagcat
     5401 gtcggtggat cttcaccgag ttacaataac aatcttgacc tcatgggggc acttcgaaca
     5461 gtcactgaac tcgccgcaac atggaaggtt aacttcgggg aagtccccta tattgcagtc
     5521 gcagtcaaac acaccaaccc atgtggagtt ggcgtaggcg ccaaaccaga gatcgcatta
     5581 aagaaagcga tagacggcaa tcgcaaagcc atcttcggcg gatgcttgat ggtaaacttc
     5641 ccgatcacaa acgagctcgc acttgtcata cgagactaca acacggctgg ggacagcaga
     5701 cgcatccttg atgcagtgat cgcatcgagc tttgacgacg aagcgattaa ggagctcgag
     5761 cgagccaaag gcaaatgccg ttttttcgaa aacggaaagc ttgccgagct cgacgagcat
     5821 tcactctata ggggacctct ctttgcgcca ctctggggag ccatcgagac ccagccaagc
     5881 gtcccgttca tccttgacct caaagacaca cgcatcgtcc gccatgggga caaactcacc
     5941 ccacagcaag agcgcgacat gctgatcgcg cgtgttgtat gcggggtcac gacgagcaat
     6001 accatatcgc tcgccaaaga tgacatgttg aagggaaacg gggccggaca gcagtcgcgc
     6061 gtcattgcgg cacgagttgc tctcatggtc gccgacgacg gcggacacaa cgcaggagga
     6121 gctgtcggcg caagtgacag cttcttccct gcaccggacg gtccagaagt ccttgccaag
     6181 gccggtatcg gcgcgatgtt cacaacaagc ggatcgcgaa gggacgacga aaccattgcc
     6241 gtcttcaatt ggtatcgcat ccctcttgtt atgcttccag acgtagtcgc tcggggtttc
     6301 cgacactgac caaccgactc acagaacttt ctgtgggttt ttttgttgac agcggaaacg
     6361 gaacatatat aatcacctgc ggctcggaga caaaaaaaca atcaaacaag gggtagtatg
     6421 cctatgaaca aaaccgttag accgaataac agcgaagtgc cagaagaaaa accgacagca
     6481 gccgccctct ggacaaacat gacagttcca gaacggttgg gttgccttga gtacgtgccg
     6541 gacacgctgt ttgaattaca agccgtcgaa gagcggcacg atcgaatgac cgaactcgca
     6601 aacagggctt tctacacact gtcacccagt ctgcgccagt cactcgagcg gcctgattca
     6661 gtactcttcg tgcattgaac cgcatgctca ccggctgcgg cttttttata aaaaaaaaga
     6721 acgatctcat gatcgttcaa ctgtctaggg ccagtcaaat gcaaggcctg atagaatacg
     6781 cgcctcccgc ggctcgtatc ccacaaaaaa agcattcgct cttgctcccc gcccccatct
     6841 ttttgttact ctaaatgaga ggcgttcttt gtcttttttc acccaaggct caaaagaaaa
     6901 tcccgtatcg aaataaaacc catcatgtag cggaaccgac agtaacgttt caactctgtg
     6961 gtctccgatg agatgcattg ctctataccg tacatgcgcg atcagccatg tttcgccccc
     7021 caactgaatc ctcgaggtca caaaaacgta cgggttttcg tggaataggt tcgccccagg
     7081 gcgcaaaaca ccagcgtcgt agagcctgcc aaggcgcgaa agaaatgact tgtcggctac
     7141 accactcgaa gaattggtgt aagaaccctc aagccacgtt cgatcggtga ccacgaagga
     7201 agcgccatga acccgaatct cgtcttcgtc aacggccgcg agcgaatttg aaaacgagtc
     7261 tgacaggaag cgaccgagaa atccaaatcg ctcttctatc gcgcttcgac ttcggacaac
     7321 aagcggagtc tcgacaacca gttcgcgcat tgaatacgaa actgacctta caaaagcact
     7381 gcccgctgtg cgttcgtact tctctccaag atcatcgtgg tcctgtagcc gcaactgcca
     7441 cttcagtgac ttgactgatc gcagactgtt ttcaaaaact attcgagtac gccgctcgta
     7501 aatctcctcc actcgatacc aaaacgatga agtatcacga attagaggaa gtctgaacgt
     7561 tacatttgaa ctccggttgg tagagggatc cttcccgcct agattctgcc gcgcattttc
     7621 cgccgccgtg gcagttgaga gtgccgaaaa agcgaacaca aagaggaata gcctgtattt
     7681 cataagagct tttctggatt tttcgatccg aaaccagcga caagtatacg ttttagttca
     7741 gcgtggtcaa tcagaaatgt tcataaaaga acttgacata aaaacgcgct ctaacaaagc
     7801 cttcttttga caaacgatga ttgtcgacag tatttaactt atgtgttgct ttatagacca
     7861 gcgcaatttt gagcggccgt ataacctgcc tcaatggctt tttcttccga agcaaaataa
     7921 attttgttct cgtcgttgat gcgactggcc gccttgcacc agggaaaata gtagcgagtt
     7981 ccattttttg aggccactac ttccccaccc gccttcaact taggtacctg cgggagctgt
     8041 tctacggcat ttgcgttttt gttggttgcg ccgagaagat tcgcctgtag caaccgtatt
     8101 ggtgacgcgc tgtttccact catggacaat cgcccgattt ggaacgaagc catcgcgaca
     8161 agagcaacaa tcaaaacgac cgccagatct ttgccgtagt gcccaataat ttttctaagg
     8221 ttcgacatag gtcttgccaa tcacagatta gtcatatata ctgcaatttc aataagtata
     8281 ccccgaagtg agctcacttc ccacgccgcg cttatgtgca aacttttggg gtttcgaata
     8341 ttattaaatt tcacggtaga ttgaaagtgt atcaagatct acttcggggt ataccaaaac
     8401 tatggcaacc aaaaaggcag gcggatcagc aaaaaacata tcgcatcaaa acccgaaata
     8461 tctcgggata aaactctatg ctggcgaaaa agccttctct ggatcgataa ttgtgcgcca
     8521 aagaggcact cgcattcttc ctggcgagaa tgtcggaatg ggaaaagacc acacgctcta
     8581 tgcagaaaaa gaaggtacag tttcatttgg cagcaagcga aaaacaaatt acgacagctc
     8641 cgtctcaata aagaaaatag ttcacgtaaa gtaaaatccg cctaaggcgg attttacttt
     8701 gtgtcccctt ccttcttcac tcacgatttc ttaacgccaa ttttgatttt tgtcatttga
     8761 tcaccgagcc taaacccaac cgtatgatct ccaatttcct tgataggctt ttcgagctct
     8821 atgtggtctt cgtcgattag aatacctttg tttttcagtt cggccgctat ttctgcttta
     8881 tgtattgccg caaagagatg tcctttctca ttagcctttc cagacatgat aatgccttgt
     8941 ttcgataacg cctgcatttt ttcggcagac tcatggagtg ctttttcaat cttagccttt
     9001 tcctgcactt tcgtctcgtc aattttttta ataagcgcgt ccgttgcatg ctcaacttct
     9061 ccacgcggaa tcaggtaatt cagcgcatga ccgtccgaga cgtttttgac atcgaatttt
     9121 ttaccaagtt tagcaacatc ttttagcaat atgactttca tcccgttagt ggcaggtcgc
     9181 ggtcgattgt tcttcccgcc ccgccttgtt ttaattgcac tgtaaaattt ttacgttcta
     9241 gatccctttt tgcgaccatg accgcggcca ctaacgggat tcatgcctgt gagtttcatg
     9301 tatcaacata tcggatttaa tttgggaaag caagcgacta caagattcta aagactcttg
     9361 acaagttcaa tggctttttt cttctgcaaa tcgtttttgg catctatgtc ttcgggcgag
     9421 agttttacct caacatccgg ctttacacct ccatcagaaa tacttatacc gtttggcgtc
     9481 agccattgcg cgatcgttac cttgagattt gtatcctttg taatcggcac aagctcctgc
     9541 acagaccctt ttccgtatgt ttgtgtaccg accagttttg caaccccgtg ttcctgaagc
     9601 gcccccgcta aaatttcaga ggccgaggcc gatcccacat cgacgagaat gacaaattta
     9661 attttatcag taaatatatt gtatcctttt ttgctcctgt agacggtttc ttctttattc
     9721 gggccaaaac tttcgcgcac aattatcttg ccctcgggca gaaaccaact tgccatatct
     9781 acagccgctt cgagatatcc tcctggatta ccacgaagat cgagcaccag cttgtcagta
     9841 ttcgcaagaa caaactcctt aagcgcattt ctgaagagat ctggggatac tgcggagaaa
     9901 ctgcgaagtc ctatcacaaa aactccgtca gtgcgctttt ccgtttctac attgggaata
     9961 gctatagtgt ctcggataat cgtaacttca aaaggctctt tcttcttttc tcggaggatg
    10021 gtcaatttta caggagtgcc ttttttcccg cgcatttttc tgattgcttc atcaatcgaa
    10081 agatcacttg tcagcgtgtc gtctattttt aaaattttgt ctccagcgag aatacccgcc
    10141 ttgtaagccg ggttatcttt gagcggcgca ataatcgtaa gtatattgtc cttcatgccg
    10201 acttcggctc cgattccctc aaaattgcca ctaatttctg tttgaaagaa ttcgctctcg
    10261 accggcggca gaaaaacagt atacggatct ccaagagacg ccgcgagacc ctctgcggct
    10321 ccccacacgc gatcctgggc ggaaatatca cctttgcttt ttttggtgct tacaaacttc
    10381 tcattgagca cattccacac tttccaaaaa ggctcaaaat ccgcctcagc ggaaactata
    10441 ttatccttat ttgtgacatc tttaactttc tctatttcag gcctatggta taagcctgta
    10501 taaacgcctg acccaaagga tatgccgata acgacaatcg cgataataat cagaaccccg
    10561 ttcgacgtaa aaaaaccgcg caattttgtc cacactgttt gaaaatgcat cccgttagta
    10621 gtagaaaatg ctcccgctca tcaggatgca tttattattt gttaataatt cagcacttgg
    10681 cgccacgcgt tcaaaaacaa caaagcattt tcgtggcata tcccgatgaa gaagcattgt
    10741 attatttaag ttttatattt taataatcga gaatacataa ctaagggact tctaacggga
    10801 tgcatacaag taagcatcat tatgccatag gtgtgaaccg agacaaaata cccacgcacc
    10861 tacgcttgca ctgtttccac aaaagtatga tggaagcgcg ttcacttcag ggcctgcccc
    10921 gtagtagacc caggtgtcgt cgaacgtgag tattttatga ataaataaat gagcgatcaa
    10981 tttcaaacag taaaaagatt gtgagggtct tactatgggg tataattgta tgaatgataa
    11041 atcggcacat ttacaaaaat ctcgtctgga tagatttaga gtctccgacg catgaggaaa
    11101 ttgaggaaat agccggacag tataaaatag gccacctcgt gacgcaggag ctcgcccgcc
    11161 cttcttttaa acctaaagtc gaaacatacc ctgattatat ttatcttatt ctgcactttc
    11221 cggtcattag acactctcat tcgctcgata ctgaccagga gattgatttc attttaggaa
    11281 aagattttct tatcacagct cactacgaca ctgtagatcc gctccataaa ttttctcgaa
    11341 tcttcgaggt caactcgatt ctcgataaga gtaatattgg agaccatgcc ggttttattt
    11401 tcttttatat gatgcgcgag ctgtatacgt cgctcgccaa cgaacttgaa tttgtcttcg
    11461 aaactctcaa ggacatcgag gcgcgtatct tcagaggcaa agaaaaggaa atggtttttg
    11521 cgctttcaaa cgtcagccgt atcttactcg atttcagaca agcaacaatt caccacaagg
    11581 atatcttgga ttcattcgac gaaccggcga gagcgcttct cggaaacgga ttcggaccgt
    11641 atgttcgcgc aactcgagat gaatatttta aagtgactaa caccattaga agtcacggcg
    11701 aacttttaag cgaactgcgg gagaccaata actccttatt aaacacaaaa cagaatgaaa
    11761 taatgaggca tcttaccatg atggcattcg tcacttttcc tctttcactc gtcgtcgcat
    11821 tgcttgccat tgataccgaa tacaatcccg ttctcggact gccccaggat ttttggctga
    11881 ttgcgggaat tatcgtcttt ggaatggcct caatcattgt ctactttaaa tggaaaaagt
    11941 ggctgtaaaa aaacgtatag cgtttagcgt atagcgttta gagaaaaaag aattatactt
    12001 ccactatacc ctttactcta aactctatgc ccttgtattt attcaacacg ctcacaaaaa
    12061 agaaggagga atttatacct ctcgagccgg aaaaagtccg catgtacaac tgcggaccga
    12121 ccgtatacaa ctacgcgcac ataggtaatt tacgcgcagt gctttttgtc gatctcctgc
    12181 ggcgagcact tgaactttca ggttacgagg tgacgcaagt gatgaatatt actgatgtag
    12241 ggcatctctc cggcgacaat gacgacggtg aagacaaaat gaccaacgca cttcgtcgag
    12301 aaggcaaagc aatgacgctt caggcgatga aggaaatcgg cgagttctat acgcagaaat
    12361 tcatggatga tctcgcacgt cttcatgtaa agaccgataa tacgaaattt ccacgggcaa
    12421 gtgagcatat ttccgaacaa ataaagctca tacagaaact cgaacaaaaa gaactcacct
    12481 accgcaccga tgacggggta tattttgaca cgtcaaaaat aagcgactac ggcaaacttg
    12541 gcggcgtctc gcgagaggca agtgtcgctc ggatcgaaat gaagtccgga aaaaaaagtc
    12601 ctcgagactt cgcactctgg aagttctctg atacgggcaa ggatgagctc ggctggcaaa
    12661 gcctctgggg cgttggcttt cccggctggc acattgagtg ttcggccatg gccatgaagt
    12721 atctcgggga aacgtttgat attcacaccg gcggagttga tcacattccg atccatcata
    12781 acaacgagat cgcccagtcc gaaaacgcaa ccggaaagcc gtttgcacac tactggctgc
    12841 acaacgcgtt tctcaatatt cgcggtgaaa agattgcaaa atctgtaggc aatgcgcttt
    12901 ttcttcataa cttaagaagt cctctcgcct atcgctatct tattctcggt gcgacttaca
    12961 gaacacaaat cgactacacg gacgaaagct atgaggccgc agagaatgca tacaaaaaat
    13021 ttataaaaga gttttcagtg ctaccgaaag agaccggcgc tgtcagcgaa tcctataaaa
    13081 aagattttga tctcgcgctt gcggatgatc ttaatactcc tcaagccctc gcgcttgcgt
    13141 ggcaacttct taaagataag tctgtacgcg cgcaagataa gcgcgccacg ctcctttact
    13201 tcgatcaaat attcggtttt aaccttgagg cagagtcgga aatttcggca aagctagaga
    13261 taccgtccaa aatagaagtc ctcgctaaag agcgagataa agcccgggcc gcgaaggatt
    13321 ttaaagaagc cgatgaaata cgcttacaaa tcgagaaaaa cggctatatg atcgaagaca
    13381 cgcccgaggg cacgcgaatc gtaagcgc
//
LOCUS       DATDSU010000102         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1926783, whole genome shotgun sequence.
ACCESSION   DATDSU010000102 DATDSU010000000
VERSION     DATDSU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926783"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..977)
                     /locus_tag="VI981_03500"
     CDS             complement(294..977)
                     /locus_tag="VI981_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY4479392.1"
                     /translation="MRKVAFFDVDGTVFRSSLLIELIEALIERDQIPEAARNMYIVAE
                     LSWKNREGSYEQYIASVIKAFMKHIKGVSYKSFGETIEEVITHQKKRVYTFTRDLILD
                     LEKKKYYLVAVSQSPKGILDAFCREYGFDKIYGRFYELGPQDRFTGGVIDEHLIMNKS
                     NIVKRVIEREKLTLKNSVAVGDTEGDIPMLDLVENPICFNPNLKLYKHAKRNGWKIVV
                     ERKDVIYEL"
     gene            1498..1568
                     /locus_tag="VI981_03505"
     tRNA            1498..1568
                     /locus_tag="VI981_03505"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1530..1532,aa:Gln,seq:ttg)
     gene            1652..1906
                     /locus_tag="VI981_03510"
     CDS             1652..1906
                     /locus_tag="VI981_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY4479393.1"
                     /translation="MAKSNSAFMKPLNVSADLAMVVGNGPMPRSEVVKKLWEYIKGKN
                     LQDPKNKRNINADANLKKVFDGKSVVNMFEMTKLVSKHLS"
     gene            1922..2377
                     /locus_tag="VI981_03515"
     CDS             1922..2377
                     /locus_tag="VI981_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479394.1"
                     /translation="MNREILDYVARERVGVLAVEMLDGWPHAAAVHFVCQEKPFIFFF
                     ETDRTYKKSEPLLSRELSRASFVLGSDESALKTFQLDGDVRVVKKVELETFESVYYCK
                     FPEKTSKAGPDSVFFIFTPRWWRYTDYKKPGGKFILSSDNAVDSDRELT"
BASE COUNT          715 a          540 c          535 g          738 t
ORIGIN      
        1 catctaagag agtccacgtt tgtgttttct tgtgaagatc gacgcctaag taatatgtca
       61 tacgatatgt atgattggtt tgtaaatatc actgacgagg tgacagcata acggtagcag
      121 agtgggtcta gaaggtcagc gagtacatct ttgtaaggaa ctttgttcat atttcttgtc
      181 tttcctaact gggtttcaga acttacccaa tcgtactttt ttcatccaaa aaagcaacaa
      241 aaaaatgctg ccattttaag gcctcattcg tgcgggaacg aaaacaataa aaattaaagc
      301 tcgtaaatca catccttcct ctccaccaca atcttccagc catttcgctt agcgtgctta
      361 taaagtttta gattcggatt gaaacaaatt gggttttcaa cgaggtcgag cattggaata
      421 tcgccctctg tgtctccaac ggctacggaa tttttcaatg taagtttctc tctttcgata
      481 acacgtttga cgatattcga tttgttcatg atgagatgct cgtcaataac gcctcccgta
      541 aaacgatcct gcgggccaag ctcataaaat cttccataaa ttttatcgaa accatactca
      601 cggcagaatg catcgagaat tcccttgggc gactgcgaaa cagccactaa atagtatttt
      661 ttcttttcga gatccagtat gagatcgcga gtaaaggtgt aaactctttt tttctggtgg
      721 gttataactt cttctatggt ctcaccaaat gatttgtaac tcacaccctt aatgtgcttc
      781 ataaacgctt tgatcacgct cgctatatat tgctcatacg atccttctct gtttttccaa
      841 gatagctcgg ccactatgta catgtttctc gccgcttcag ggatttgatc ccgctctata
      901 agcgcctcaa tcaattcgat aagtaaactc gaacgaaata cggtcccatc aacatcaaaa
      961 aaagcaactt tgcgcataat gcaattttat cagagattct ggcatctttt aactaacgcc
     1021 ccttctgcac tggaaacacc cgatcgctct aatcatgtag tttatgctcc tgccatgtcg
     1081 cacattcgcg cgaatgtgcg aataatgata ctgtatgcag tatgaaaaat ttagaaagat
     1141 acctcagggc attggccaat aagcgtagac ttacaattat gcaattttta aaaaaggagc
     1201 gtagggccac agttggcgag attgctcgtg ctatcaactt atcggttaag tcgacatcaa
     1261 agcatctttt gaagctgggt gccgccgatt tggttgattc tgatcaacaa agtataaatg
     1321 tctggtataa actggcgccg cctcccaaat cagtttcatc cataatctcc tgtctttaaa
     1381 gcgacgacta gctatttaga cgcgtgcttc tcacacatac tcgcgaatgt gcgattagaa
     1441 attaattgat atttcgctaa attttcagta tcgtacagtg tacattctgc gcatagctgg
     1501 gggttcgtct aatggtagga ctctgggttt tggtcccagc tatcggggtt cgagtccctg
     1561 acccccagct atggacggaa taaatatgag agagtgcgat ttctcgattt agtgttataa
     1621 tcaattcact taataagtaa cattttattg tatggcaaaa tcgaattccg cgtttatgaa
     1681 gccgctcaac gtcagcgctg accttgcaat ggtagtggga aatgggccaa tgcctcgttc
     1741 tgaagttgta aaaaagcttt gggaatacat aaaaggcaaa aatcttcagg atccgaaaaa
     1801 caagagaaat atcaatgcgg acgcaaacct caagaaagtt tttgacggaa agagcgttgt
     1861 gaatatgttt gagatgacga agctcgtttc caaacacctg tcataatagt aaacacaaga
     1921 gatgaaccgc gagattctcg attatgttgc tcgagaacgt gtcggggtac tggcagtcga
     1981 aatgctcgac gggtggccgc atgcggccgc agttcatttt gtgtgccaag agaaaccgtt
     2041 tatattcttt tttgaaactg accgtaccta caaaaaaagt gagccgctgt tgtcgcgtga
     2101 gcttagtcgt gcgtcatttg tacttggctc cgacgaaagc gctctaaaga cgttccagct
     2161 tgatggggac gtacgggtag tcaaaaaagt ggagctcgaa acttttgagt ccgtttacta
     2221 ctgcaaattt ccggagaaaa caagtaaagc cggaccagat agtgtttttt tcatttttac
     2281 accccgctgg tggcgctata ctgattacaa aaagccaggt ggtaaattta tccttagttc
     2341 agataacgca gtcgacagtg atagagagct tacatgacaa caagagcaat ttttagtctt
     2401 ctgtcttcgg tcgtgttact agccggtgca ataccctact ttatagacat tcatcacgga
     2461 aaagcgcgcc cccatatttt aagctggatc gggttggcgt ttattacagc gcttggagca
     2521 agcgcaat
//
LOCUS       DATDSU010000103         5202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2114806, whole genome shotgun sequence.
ACCESSION   DATDSU010000103 DATDSU010000000
VERSION     DATDSU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114806"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1556
                     /gene="tsaD"
                     /locus_tag="VI981_03520"
     CDS             408..1556
                     /gene="tsaD"
                     /locus_tag="VI981_03520"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY4479395.1"
                     /translation="MIILGIETSCDETSLCLIKYGRNTIEIQGDETLTQTVHAEYGGV
                     YPNLAKREHAKNLIPLFKRVLSTHTVVHHPGSVPLREYTELKDIFSKEPELYKSFLDY
                     IPTIEKPAIDAIAVTHGPGLEPALWVGVNFARALSLVWNIPLYPINHLEGHIRVALLH
                     QISESQFSITKPAFPAIALIVSGGHTELVLMNDTVNFHVIGATKDDAVGEAYDKVARI
                     IGLRYPGGPKIEKLAEQYRSERLKSPFVFPRPMIASNDLNFSFSGLKTAVLYKAKELK
                     NIDENAKKIIAFEFEQAVIDVLSAKTKKALEIHGAKSLILGGGVVANGAIRHSFLQLG
                     AKMSIQTFIPQLAHSTDNAFMIAVTGGFHVGNKKPSAFFEAEGNLKLE"
     gene            complement(1557..2558)
                     /locus_tag="VI981_03525"
     CDS             complement(1557..2558)
                     /locus_tag="VI981_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479396.1"
                     /translation="MKLHITVGIVGLFLASALFAHAETLPQVVLTATQTASSSDEATS
                     AVTVATVSSPMVTQVGAYLVEEGSMGEVTIRWANTSSDVPWVFFDIKSHASETRAVYP
                     FVESATTVSGMSRVKISGLTPGKTYYFRVGGSRSTFAFAASEFSYAIHYPQTEPCRLL
                     NAPLGSGKANDTTEVIKLQQFLRYGEHLEVDMNGSFDQVTEAAVVAYQEKYKGEVLAP
                     WNLSQGTGYVYITTLKKINSSFCGKTMTLTADELEIIRTGSTMHQLTVQNTTPSVGIG
                     PLPGLDVVTPTTPPPASSVKATTSTTVKKANPVSSFFKRLFGRGSKATTTTPTATKK"
     gene            2892..5057
                     /locus_tag="VI981_03530"
     CDS             2892..5057
                     /locus_tag="VI981_03530"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY4479397.1"
                     /translation="MSSNEQQPIEIDEKLLSPYKPQEVEERIYALWENSGFFNPDNLS
                     NHGKPFTIIMPPINANGSLHAGHGLVMTIEDIMVRYKRMQGFKVLWLPGLDHAGFETQ
                     VVYEKQLEKEGRSRFSMEPKDLYNEIMKFTLQNSKNIKSQIRKMGASCDWSREKFTLD
                     ADVIKTVYDTFKKLSEDNLLYRGKRIISWCPKHRTSFSDLEIKDEERKDPFYYLKYGP
                     FTIATARPETKFGDKYVVMHPDDTRYSSFKEGQKIDLEWINGPITATVIKDSVIDMSF
                     GTGVMTITPWHDATDFEIAERHKLDKEQIIDESGKLLPIAGEFAGLSINKARPLIVEK
                     LKKKGLVEKVEEEYKHAVRTCYKCGTVIEPQIRSQWFIKMKPLAERALQKIQSKEITY
                     IPEQYEKITTHWLLNILDWNISRQIVWGIPVPAKICDHCDRGFVDLENEIKTCPECGG
                     VVRQDPDTFDTWFSSGQWPYASLGFPKNHDFDMYYPTDIMETAGEIIFFWVSRMIMLG
                     LYVTGKIPFKTVYLHGLVLDAKGQKMSKSKGNVINPLNLTEKYGTDALRIGLVMGNTP
                     GTSLALSEDKIRGYKHFANKLWNISRFILSNTAGVLLDPKFTDYTDSDQKLRQERHAL
                     LVDVTADMDGFRFYLAAEKLYHYTWHQFADKILEESKPIFESSDANAIASRQQLLLHT
                     IIKLLTSLHPFMPFITEELWQIVHKGKAGLLMIAPWQIK"
BASE COUNT         1521 a         1158 c         1145 g         1378 t
ORIGIN      
        1 tagcatatac cagcgatcgc tggtatatgc taggtaggtt ttattcaaac aatgaacctc
       61 ccgcggcaag ctgtcccctc actccgtttc gctccttatc ggcgggttat ctaggagctt
      121 cgctccggat taccttggaa accaaggttc ccaaaccttc cttagcggag acccacgagg
      181 cagatcctcg gagtatatgc ttcgctaata aaacatacgg aggttgtaca ataaaatcca
      241 ttcagttaat gtagtctcat aagtctcaaa attaatgaat attctgaaat taacatgcgt
      301 attttaggta tagagacccc agtagtagat ccgcagggcg gctcactacg gggcaagaag
      361 ttccgacaca aaattaatct gccatatttt ttaggctaca tattattatg atcattttag
      421 gtatagaaac aagttgcgat gaaacatcac tctgcctcat caaatacggg cgcaatacaa
      481 tagaaataca gggcgacgaa acgctgacgc agactgtgca tgccgaatac ggcggtgtgt
      541 atcctaatct tgccaaacgg gagcatgcaa aaaatttaat accgcttttc aaaagagtcc
      601 tatcaacaca tactgttgtc caccatccgg gttctgtacc cttacgagaa tacacagaac
      661 tcaaggatat tttttccaaa gagcccgagc tttataagag ttttttagac tatataccaa
      721 caatcgaaaa accggccatc gatgcaatcg cagtgactca cgggccaggt cttgagcctg
      781 ctctgtgggt cggagtaaat tttgcaagag ctctttctct cgtctggaat ataccgctct
      841 atcccatcaa ccatctagaa ggacatattc gtgtagcttt gctacaccaa atttccgagt
      901 ctcaattttc aattaccaaa cccgcgtttc cggcaattgc tcttattgtg agtggagggc
      961 acacagaact tgtcttgatg aacgacacgg taaatttcca tgttatagga gcaacaaaag
     1021 atgatgcagt cggagaagcg tatgataagg tagctcgaat tattgggctt cgttatccgg
     1081 gcggccccaa aattgaaaag ctggccgagc aatatagatc agaacgattg aaaagcccgt
     1141 ttgtctttcc tcggccgatg atcgcgagca atgacttgaa tttttctttt tcaggcctga
     1201 aaactgccgt gttgtataag gcaaaggaac ttaaaaatat cgacgagaat gcaaaaaaaa
     1261 taatcgcctt tgaatttgag caggcagtaa tagatgtcct ttccgcaaag acaaaaaagg
     1321 ccctcgaaat tcacggggct aaaagtctaa tacttggagg cggtgtcgtc gccaatggcg
     1381 caatcagaca ttcatttcta caactcggag caaagatgag tattcagacc ttcattccac
     1441 aactggcgca ttcgacagac aacgctttta tgatcgcggt aaccggtggc ttccacgttg
     1501 gtaacaaaaa accctccgcc tttttcgagg cagagggtaa tttgaaactc gagtagttac
     1561 tttttggtag cagttggagt tgtcgttgtc gctttacttc cacgtccgaa taacctttta
     1621 aaaaatgaac tcaccggatt ggctttcttg acggtcgtac ttgtagtagc tttgacagat
     1681 gaagcgggcg gcggcgtagt aggcgtcaca acatcaagtc cagggagcgg accaatccct
     1741 acagaaggtg tcgtattctg gaccgtcagt tgatgcatgg ttgaaccagt tcgaatgatt
     1801 tcgagctcgt ctgccgtaag tgtcatcgtc ttaccacaaa atgaactgtt gatcttttta
     1861 agcgtcgtta tatagacata gccggtacct tggcttaaat tccacggcgc caaaacttca
     1921 cctttgtatt tttcctgata cgcaacaaca gcggcttcgg taacttgatc aaatgatcca
     1981 ttcatatcga cttcaagatg ttctccgtat cggaggaact gctgaagctt gatgacttcc
     2041 gttgtgtcat tcgccttacc agagcctaac ggagcgttaa gcaaccggca gggttctgtc
     2101 tgcggataat ggatagcata actgaattca gaagcggcaa aagcaaatgt gcttctcgac
     2161 cctccaacgc ggaaatagta ggttttccca ggtgtaagtc cagaaatctt gactctagac
     2221 atacctgaca ctgtcgttgc agattctacg aagggataaa ctgctcttgt ctcgcttgcg
     2281 tgactcttta tgtcaaaaaa tacccaaggc acatcagatg atgtgttcgc ccagcgtata
     2341 gtgacttctc ccatacttcc ctcttcgacc aaatacgctc ccacttgtgt caccattggg
     2401 gaggacacag tagctactgt gacagcactc gtcgcttcat ctgaggatga tgcagtttga
     2461 gtagccgtca gaactacttg cggaagggtt tcggcgtgcg caaacagagc cgaggccaaa
     2521 aacaatccga caattccgac agtgatgtgt aatttcataa gctattaacg attctctgat
     2581 ataccgctag cctaatactc gacagggcaa agtcaagatg acaaatactc aatttatggc
     2641 tctgtaaagc caagattcaa tatgtccttt acaaaacctg gcagcctcaa tgtcctaaac
     2701 aaacgactaa gaatgagcac agcgtaaagg acattttgcg cttgtttgtt tttgctcgta
     2761 tgacttttat atgtacttta aactcaaaaa acaatagaaa acatttatta ttttagcgca
     2821 agaactctct cctctttttc ttttggattt gtaggttttt cctgttacca ttgagacact
     2881 agtttttgaa aatgtcgagc aacgagcaac aaccgattga aattgacgag aagctgcttt
     2941 ctccctataa gccacaagag gtagaggaac gcatttacgc gctatgggaa aatagcggtt
     3001 ttttcaatcc tgataatctc tccaatcatg gaaagccttt cacgatcatt atgcccccca
     3061 tcaacgccaa cgggtcactt cacgccggtc atggacttgt catgaccatt gaagacatca
     3121 tggtgcgcta caaacgcatg caaggcttta aagtactgtg gcttccggga cttgaccacg
     3181 ctgggttcga aacgcaagta gtatacgaaa aacaactcga gaaagaaggg cgttcacgct
     3241 tcagcatgga acccaaagat ctctataacg agatcatgaa gttcactttg cagaattcga
     3301 agaacattaa atcccaaatt agaaaaatgg gggcttcatg cgattggagc cgtgaaaagt
     3361 tcacgcttga tgcggacgtc ataaaaactg tatacgatac cttcaaaaaa ctatccgagg
     3421 ataatcttct atatcgcggc aaaagaatca taagctggtg tccgaaacat agaacttctt
     3481 tttcggatct tgaaataaaa gacgaggagc gtaaagatcc gttttattat ttgaaatacg
     3541 gaccattcac gattgccacg gcgcgcccag aaacaaaatt tggagataag tatgtcgtca
     3601 tgcatccgga cgataccaga tatagttctt tcaaggaggg acaaaagatc gatcttgagt
     3661 ggatcaatgg gccgataaca gcaacggtaa ttaaagattc ggttattgac atgtcatttg
     3721 gaacaggtgt catgaccatt acgccatggc acgacgcaac cgacttcgag attgcagagc
     3781 ggcacaaact cgataaagag caaatcatcg atgaatcggg gaaactgctt ccaattgcgg
     3841 gtgagtttgc cggcctatca atcaataaag ctcgacccct aatcgtcgaa aaactcaaga
     3901 aaaaagggct tgtagagaaa gtagaagaag agtataaaca cgccgtacgc acttgctaca
     3961 agtgtgggac agtcattgaa ccacaaatac ggagtcaatg gtttataaaa atgaaaccgc
     4021 ttgccgagcg tgcgctccaa aaaattcaat caaaggaaat cacctatatt ccggaacaat
     4081 atgaaaaaat aacgactcat tggcttctaa acattctgga ctggaacatt tcaaggcaaa
     4141 ttgtgtgggg tataccggtt cccgctaaaa tctgtgacca ctgcgatcgt gggtttgttg
     4201 atctcgaaaa cgaaattaaa acctgcccgg aatgcggcgg cgtggtgaga caggaccccg
     4261 atacttttga cacgtggttt tcgtctggtc agtggccata cgcctcgctc ggatttccca
     4321 aaaatcatga ttttgatatg tattatccaa ctgacataat ggaaacagcc ggggagatta
     4381 ttttcttttg ggtttcaagg atgattatgc ttggacttta tgtgaccgga aaaattccct
     4441 tcaagacagt ctatttgcac gggctcgtgc ttgacgcaaa agggcaaaag atgagcaaga
     4501 gcaagggcaa cgtcataaat ccccttaatt tgacggaaaa gtatggcact gacgcgctgc
     4561 gaatcgggct tgtgatggga aacactccgg ggacatcgct cgcgctctct gaagataaaa
     4621 tcagagggta taagcatttt gcgaataaat tgtggaatat cagccgtttt attctttcaa
     4681 acacagcggg ggtcttgctt gacccaaagt tcactgatta cactgattct gatcagaaac
     4741 tgcggcagga gcggcatgcc ctgctcgttg atgtaactgc cgatatggat gggttccgct
     4801 tttatctcgc cgcagagaag ctgtatcatt atacgtggca ccaatttgca gataagattc
     4861 ttgaagaaag caaacctatt tttgaatcga gtgatgcgaa cgccattgcc tcgcgccagc
     4921 agttattgtt gcataccatt attaaactct taacttcatt acaccccttt atgccgttta
     4981 tcaccgaaga attgtggcaa atcgttcaca aaggcaaagc cggtctcctc atgatcgctc
     5041 cttggcaaat taaataatga attcaataat tttcttcata aaagatattt tggattacag
     5101 catgattacg gtgttcaaca ctccggacag gttttttctg gcgctaactt tcggaatact
     5161 gccggcactg ctgtggcttt ggttctggct tcacgaggat cg
//
LOCUS       DATDSU010000104        14956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_934078, whole genome shotgun sequence.
ACCESSION   DATDSU010000104 DATDSU010000000
VERSION     DATDSU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934078"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..848
                     /locus_tag="VI981_03535"
     CDS             21..848
                     /locus_tag="VI981_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4479398.1"
                     /translation="MNTLDTFNLNGRIALITGAAGLLGEKHAEAILDGGGRVVLTDIN
                     TTLLDEKARQLVKKYGKNKVWQYTCDITDENAVKSIIEKINMEAGPIDILINNAANNP
                     KVEELQKNFSRLENFKREMWDKDIAVGITGAFLLSKYIAPEMAKRGKGVILNIASELG
                     VIAPDQRLYRNQQAAENNQPVKPVTYSVVKHGIIGLTKYLATYWLGEKVRCNAVAFGG
                     VENNTPKDFLDRIGKLSVMGRMAKPDEYKAAVLFLCSDASSYMTGSVITIDGGRTVW"
     gene            842..1429
                     /locus_tag="VI981_03540"
     CDS             842..1429
                     /locus_tag="VI981_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY4479399.1"
                     /translation="MVEKLTISKWAREYIEKSARANASIDLEAVRAIIQKVAEAGRNG
                     RKIFVIGNGGSASNASHFATDLGKSASDAVGKRFFILSLNDNTSWITALGNDYSYDEI
                     FSGQLKNYANTGDLLIVLSVSGSSPNLVKAAELAKTMGLHTIALIGGKRGTISKVADT
                     VVVVEDTHYGRVEDAEMTICHMICYALIEHPEWAD"
     gene            complement(1471..1968)
                     /locus_tag="VI981_03545"
     CDS             complement(1471..1968)
                     /locus_tag="VI981_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY4479400.1"
                     /translation="MNEELRNKISRIKLIGTDFDGVWTDGKVTTDQNGNESVTCSRKD
                     TLRIKDVKAAGIEVFVISKEQNAVVARRCEKMKVEFKQGIDGKLAVFKALLADRGLTP
                     DEAIFIGDDTNDLDCLKHAGLAMTVADGDPLCKAEADYITTRNGGDHAMREIFDLVLS
                     VRAPQ"
     gene            complement(2007..3389)
                     /locus_tag="VI981_03550"
     CDS             complement(2007..3389)
                     /locus_tag="VI981_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479401.1"
                     /translation="MMVSLKQKFFYGETSFALWDIVSKAIGLLNTFFILKSLSLYEYG
                     VFQLLLSGYAFISNFTSPAGNAVGNDISRLIGERREPEAKKLFWEYNRLRILGGGILC
                     LVAFFGSYLFLFKYDYNFIINLQLLAVITMLEILFTTLKAVLRLRLFFGQVASRSTFY
                     KVAQLIFLSASYFFFNLGTTEVLWSMIAGSLLSSLLLVPVVARAYGPWRGLAMSNQKF
                     LIPILKSQGKWEIFSVGFSKLSTYFQPWITKILINTEAVAIFSIAQTMLGTIYSFFPT
                     KTISALVPLKMEDKELIQRVYTIAVKYLVFFGVACGLVALVLSGPVIRQFFEQYTVSL
                     PYFYALLPIIPVTAASLVTSAFLVAHRKQKFLFLEKILKSAIGIPLYFILLPLFDIWG
                     LVAQSLIVSVVLFSVMYFYTVKWQVGIKLELRRILSFGAEDREFVKKFMESTVNFIKL
                     KFRFLRNNLT"
     gene            complement(3386..4762)
                     /locus_tag="VI981_03555"
     CDS             complement(3386..4762)
                     /locus_tag="VI981_03555"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEY4479402.1"
                     /translation="MTKTIFVDLFAINAARNLIMPDGVVDLLTRPGDMRVVLLIPNLP
                     EFEALVSPYSSRPHVVVEKISKTFPETFLEKAFQFFYSYLVFTPTTALLATFGARADA
                     PPAGGNRHLWIVKAFIANTFGRFKLLKRKIVPAIYMRIFHTRPYSYLFSKYHPQLVFL
                     PNVAFNPGIELTAESKRQKVRTVAMPANWDHLNKYYIPTHADFLLVQNEPMMREAIDY
                     HAYRKEQIVPVGFPQFDDYTRRAYVRPREEFFEKFGFNKDERFILYISGSVYAMDEAD
                     ILAELYAWIKAGKFGPGVRLMVRPYVGLREIELEKKKYEKLMQKPDIIFNWRTNDENS
                     ENKKDFTSMLYYSSLIISVFSTTAIEAALFDKPIITLGFDGYSKQPYHKSIARLERLS
                     HFKHVLDTGSVRVARSFRELEELASLYLKNPELDKDKREILTKKMCYMADGHASERIA
                     EFILNKAK"
     gene            4905..6122
                     /locus_tag="VI981_03560"
     CDS             4905..6122
                     /locus_tag="VI981_03560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4479403.1"
                     /translation="MRIAMIFRKLNVKGGTQRQGLSLARELKKMGHDIKIYTLNLDRE
                     KCYPELLNLFEVKTLKDNEKDAPYYPMFLTKIGVTEVSKENKLAQDLAEIMDDNFDLL
                     NPHDQVSYKVAYYYKKQKKNIPAVWNMNDLPLKQWGYARMKGCDERFHQPWYRHLAYK
                     LFDFYDKHKFIKHMDKIVVVDFFNRELVKKYLGLKADTVRSGPDFEHFIFEAKSHPGK
                     KMKILTSGILMPHRRFEDTIAALPILQKRGYSPTLTIIGDTNNDKKYFARLGTLVEEL
                     NLKNVVKFTGRVSEEDLVKSYQTHDLYIFQHHLQSDGLSSFEAAACGMPFIVSKTAGC
                     HEVLTHGENALLIEAKNPDDIADKVEQLIKDPELYAKLAKNAHAFVHENFSWPKYAKG
                     VLDIMELALRKYE"
     gene            6115..6834
                     /locus_tag="VI981_03565"
     CDS             6115..6834
                     /locus_tag="VI981_03565"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4479404.1"
                     /translation="MSDNYSNEGDATLLSLDQAHNFTDWMYAQIKPYLRGDILEIGSG
                     IGTYSKKTIRDFSQSRICLSELDPVYCKKLSEQNFGNSRIEIRSLNLEQETDFLPISQ
                     KFDCAFALNVIEHVNEHELALKNVYRALRPGGVFIVLVPAHPLLFNNLDRAFGHYRRY
                     TRRGFKELVARTAFKIEHLFMFNALSMLGWFVNGTLFRKSVLHDKTLASFNKLVPIIR
                     PIETFILQKKIGISLIAVLKK"
     gene            complement(6870..7580)
                     /locus_tag="VI981_03570"
     CDS             complement(6870..7580)
                     /locus_tag="VI981_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY4479405.1"
                     /translation="MRVKSYGRGSYVHIIQRGTRNLPIVLDDHDRKNFLRSLFYLNRT
                     DVPQNWKRDCLTYSRNFPMQFPPKWHTAKSLVSILAYALVENHFHLILEESTENGISR
                     FMHKMCTSLAKSYNEKNKTVGTLFQSSYKAKIIDTDSYMRIISVYVQVKNGFDLLPGG
                     LTQAKKSFQRSFDKVLENPYVSLGDYLGTRRTNILTLDGLFSSIHGGAGSYKNFAKEY
                     IKNYKKTDTAELELLDRI"
     gene            complement(7604..8479)
                     /locus_tag="VI981_03575"
     CDS             complement(7604..8479)
                     /locus_tag="VI981_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4479406.1"
                     /translation="MRILVTGALGLLGKEISSFKQNDFEIIALSHKDIELTNQTSADE
                     AISKYKPDAIINTAVIVSVDKCEADEKLCFDVNTRGVEYLIYAFQKSGARGPFIQISS
                     SEVFGRVREGEYKIEGYSEEEKPMPVSAYQRSKTEAEEIVKMAGEKQKTPWYVVRAGW
                     LYGKGRETFVEQFIKKLQEEKEVEVIVDQWRSPTWTGDFVKSLFDLIKSKRESGIYHI
                     APDIKVGEATTMDVINEISEFLGKKAKATYKMVKRDSVFKVPRAPSNVILNTKLPKLP
                     YWRDSLRNYLQSLYN"
     gene            8630..9418
                     /locus_tag="VI981_03580"
     CDS             8630..9418
                     /locus_tag="VI981_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479407.1"
                     /translation="MNEKVVLAMVTYYPDPEGRDKIRFTLALNTVRNAVAAEHGVVVV
                     DDSPSLPVSGPYVSKRLAEVGARVFPQLHRGLGPAKRQSFFHALELCVARSHAGILAT
                     EAEKDMAPDIPRMLAALEQQDCSICVPRRTSETWPTWPTHQQRTEKEANAGFEAATNR
                     NFDVMFGPVLFTLEAASHFVIQEPAKVGFPDQYAQHFALEQATFAGRRICECDSSFTY
                     PAAQRAEEEGALSDEMRQKRIRQRDTLITGYEHVARFMKQLAQS"
     gene            complement(9649..11844)
                     /locus_tag="VI981_03585"
     CDS             complement(9649..11844)
                     /locus_tag="VI981_03585"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY4479408.1"
                     /translation="MPKRQKRALITGITGQDGYYLTHFLLKKKYVVFGLCRSEKAAMH
                     YFRDFSLEEKKSLTFIEGDLRDLATLRKAVETSKPDEVYNLGGQSDVKKSFEIPEETM
                     EVNYYGLARLVNETVRVNSKARLYQASTSEMFGNREGARDEKTPFAPVSPYGVSKVRA
                     HEDFVVGARARGVYISSGILFNHESPKRREQFVTKKIVRAVARMAEGGDDVLELGNLD
                     AKRDWGYAGDYVEAMWLMLQQDKPDDYVIATGTARSVRDFVSAAAKAAGLKLTWKGSA
                     IRETALDQHGKVRIRVNKSFYRPTEIYEVIGDSSKAKRELGWTPEVTFEELVERMVQE
                     EITVLTSGNAELIRLVHSTFYNNDSTKRRLADFVIRSQTLSMGKETKTFELNFAKKQG
                     ARFALMTGSGSAANLLLVQTMMNLGRWKKGDRIGVSALTWSTNVMPLISLGLTPVALD
                     CSLQTLNISPQILEKEINAIDGLFITNALGFSDDIKKIRALCDRRGIALLEDNCESLG
                     SRVSGRLLGNWGLASTFSFFVAHHLSTVEGGMICTDDVELYHALLMCRAHGWDRNLPE
                     PVQKELRKKHGVDDFYAKYTFYDLAYNARPTDMQGYLGQIELEYWDIIVTKREASFKR
                     FESVIEKNPDFLPLNMSHMDFISGFAIPVICTDEKVFKKYKEKFEKANVEIRPIITGD
                     ITKQPFYKKYVKEKTICPNADFLHRSGFYFSNSPGLTGEETERLLKLLQ"
     gene            complement(11846..12865)
                     /gene="gmd"
                     /locus_tag="VI981_03590"
     CDS             complement(11846..12865)
                     /gene="gmd"
                     /locus_tag="VI981_03590"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY4479409.1"
                     /translation="MPAKKYSKPKKALVTGITGQDGSYLTELLVSKGYEVHGLLRRSS
                     TFHTGRIGYIIPQKQAEAHVFLHYGDLADSSNLARLIEKINPDEIYNLGAQSHVRISF
                     DVPVYTGDVTGLGTLRLLEAIRDTRIKTKFYQASSSEMFGRVGEVPQKETTPFYPRSP
                     YGTAKVFAYWTTVNYREAYNIFACNGILFNHESPRRGENFVTRKITISIAEILAGKRK
                     YIYLGNLEAKRDWGYAPEFVEAMWLMLQQDKPDDYVIATGETHTIRDFLEEAFKIAGI
                     LKWEKYIKIDPRYYRPTEVDHLLGDASKARKQLGWKPKMKFKELVRIMLVADAKRLGV
                     EDKLT"
     gene            complement(12949..>14956)
                     /locus_tag="VI981_03595"
     CDS             complement(12949..>14956)
                     /locus_tag="VI981_03595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479410.1"
                     /translation="NLPGENWKTEALGNGRFWLDRIKKVPCDVYLFNTPVLPFWFKPK
                     RSIVIALDYPYNYLKATSIKDSLRRILVRWYHHRSLKRAEHIIAVSHSTKNDTMRFYN
                     IPSDKISVVYHGYKDVCKLPEKKVVTPSRFFFFAGTIKERKNILNIIKAFLIFSDSEA
                     GVGFSLVLGGKKEGAYCEEMQRFVQEHNLNEKVLFLGHLKEDELSYVYKRSEGLVFPS
                     IVEGTGFPILEAMAGGVPVITSNIFGPAELGAHGSAFLVDPYNAAEIAASMTTIVTNP
                     EARARHISAGFEQVKKFNWTNTGKETRELLERTARSLRITYLTHNVRLDNGGGVLSRH
                     IVDGFRSRGATVHLFTSLFSKLPGEEKLVMTPFSFFQTLFRLRRAARASDVIHAFDAY
                     PYGFLAWLVNNGLGKKIVLTLVGTGTLHFLRRRFIGFFARAALKGAYARVAISTFIKK
                     EVEKIAPRISLGVITPGVERNMPVADDHRARMYKPYILSVGAIRWRKGYKRSIEAFAE
                     VTREFPELNYVIVGKKYKDGYYDELQKIIEKHDLSKRVHVISDAEEAELQGFYKNAEL
                     FCLLSQNFGSDVEGFGIVFLEAALHGLALVGTSDCGVEDAVAEHENAFLVEYTDVSGF
                     AHAILQILKDPALRKKMGEKSLSFVQKFDWKNKIDEYERLYRGR"
BASE COUNT         4242 a         3501 c         3093 g         4120 t
ORIGIN      
        1 tgaattagta tattagtagt atgaacacgc tcgatacatt caatttaaac ggcagaattg
       61 cgctcataac gggcgccgcc ggtctacttg gcgaaaagca tgccgaagcc attttggacg
      121 gcggcggaag agtcgtactg acagacatca acacaacgct gcttgatgaa aaagcgcgtc
      181 agctcgtaaa aaaatatggc aaaaacaaag tatggcagta tacgtgcgat atcactgatg
      241 aaaacgccgt aaaaagcata atcgaaaaaa ttaatatgga agctggtccg atagatattc
      301 tcataaacaa tgccgcaaac aaccctaaag tcgaagaact gcaaaagaac ttttctcgac
      361 ttgagaattt caagcgcgaa atgtgggata aagatattgc ggtcggtata acgggtgctt
      421 tccttctttc aaaatacata gcgccagaaa tggcgaagcg cggtaaagga gtaatattaa
      481 atatcgcatc cgaacttggc gttatcgcac ccgatcagcg cctttataga aaccagcagg
      541 ccgcggaaaa taatcagccc gtaaaaccag tcacatactc cgtggtaaaa catggaatta
      601 tcggcctgac aaaatatttg gcgacgtatt ggctcggaga aaaagtgaga tgcaacgctg
      661 tagccttcgg cggcgtcgaa aacaatacac ctaaagattt tctcgatcgg attggtaaat
      721 tgtcagttat ggggcggatg gcaaaaccgg acgaatataa agcggcagtc ctatttctct
      781 gctctgatgc ttcctcttat atgacagggt ccgttattac catagatggc ggcagaactg
      841 tatggtagaa aaacttacta tcagtaaatg ggctcgggaa tacatagaaa aaagcgctcg
      901 ggcaaacgct tcaattgatc ttgaagcagt tagggcaatt atccaaaaag tcgcagaagc
      961 gggacgtaat gggagaaaaa tttttgttat cggaaacggc ggaagcgctt caaacgcatc
     1021 acacttcgcc accgatcttg ggaaaagtgc gtccgatgca gtgggaaaac gtttttttat
     1081 attgtctcta aatgacaaca caagctggat tacagcactc ggcaatgatt attcgtatga
     1141 tgaaatattt tcgggacaac taaaaaatta tgcgaacaca ggtgatctcc tgattgtgct
     1201 tagcgtcagc ggctcatctc ccaatctcgt caaagccgca gaactcgcca aaaccatggg
     1261 ccttcacaca atcgcgctta tcggcggcaa acgaggaacc atatccaagg tcgccgacac
     1321 ggttgttgta gtcgaggata ctcattacgg ccgcgtagaa gacgctgaaa tgaccatttg
     1381 ccacatgatc tgctacgccc tgatcgagca tccagaatgg gccgactaaa aaatccgcta
     1441 caagtgagcg gactcgagtt ttgacttgct ctattgagga gcgcgaaccg ataaaacaag
     1501 atcaaagatt tcacgcatgg cgtggtctcc cccattcctc gtcgtaatgt agtccgcctc
     1561 cgctttgcaa agcggatctc cgtcagcaac tgtcatggcg aggccagcgt gctttaggca
     1621 gtcgagatcg tttgtgtcat caccgataaa gatggcctca tctggcgtga gaccgcggtc
     1681 cgcaagcaga gccttaaaaa cggcgagctt gccatcaatg ccctgcttga attcaacctt
     1741 catcttttcg cagcgtctgg caacgacagc attttgctct ttcgagatca caaatacctc
     1801 aattccggcc gccttcacgt ccttgattcg aagcgtatct ttacggctac aggtaacgct
     1861 ttcattaccg ttctggtccg tagtcacctt tccgtcagtc cagacaccgt caaagtccgt
     1921 gccgataagc ttgattcgtg aaattttatt tcttagttct tcattcatat gttgtcactc
     1981 ttcgttaatc atgcattcaa aaataattat gtcaaattat tccttaagaa tcgaaatttc
     2041 aatttgatga aattaaccgt gctttccata aactttttaa caaactctct gtcttccgcc
     2101 ccaaatgata atatccgcct caactcaagt ttgattccta cttgccattt tacggtatag
     2161 aaatacatca cactaaagag cactaccgat acgataagtg attgcgcaac cagcccccaa
     2221 atatcaaaaa gcggcaacaa aataaaataa agaggaattc cgatggctga ttttagaatt
     2281 ttctccaaaa ataaaaattt ctgttttctg tgggcgacta aaaatgcaga agttacgaga
     2341 ctcgccgcag tcactgggat aattggtaac agagcataaa aatagggaag agacacagta
     2401 tactgctcga aaaattgtcg tattaccggc ccggagagaa caagggccac aaggccgcac
     2461 gccacaccaa agaaaactag gtatttgacg gctatcgtat atacgcgttg tatcaattcc
     2521 ttatcttcca tttttagcgg cactagcgcg gaaatagttt ttgtcggaaa aaagctataa
     2581 atcgttccga gcattgtctg ggcaatactg aatatggcaa ctgcttccgt atttattaaa
     2641 atttttgtaa tccagggttg aaaataagta gacaactttg aaaaaccgac gctaaaaatt
     2701 tcccatttcc cctgtgactt gagtatcggt attaaaaact tctgattaga catggccagt
     2761 cctctccatg gcccgtaggc cctggcaact acgggaacta gcagaagtga cgagagaagt
     2821 gacccggcaa tcatcgacca gagaacttct gtggtaccga ggttaaaaaa gaaatatgaa
     2881 gcagataaaa atataagttg agcaacttta taaaaagtcg atcgcgacgc cacttgtcca
     2941 aaaaaaagtc tcaacctcaa aactgccttc agggtcgtga ataatatttc aagcatcgta
     3001 atcacagcaa gcaactgaag gtttataatg aaattgtagt cgtacttgaa caaaaacaag
     3061 tatgatccaa aaaaggcaac tagacacaag atgcctcccc ctaaaatacg gagcctgtta
     3121 tattcccaga aaagtttctt tgcttcaggc tctcgcctct caccgatgag ccgagaaata
     3181 tcgtttccta cagcattgcc cgcaggggat gtaaagtttg aaatgaaagc atatccactt
     3241 aagagcaatt gaaaaacgcc atattcatag agagaaaggg attttaaaat aaaaaatgtg
     3301 tttaacagac caatagcttt tgaaacaatg tcccacaatg cgaaacttgt ttctccgtaa
     3361 aaaaatttct gtttgagtga taccatcatt tcgctttatt tagtataaat tccgcgatac
     3421 gctctgatgc gtggccatca gccatataac acattttctt tgtcagaatc tctcttttat
     3481 ctttatcgag ctcgggattc tttaaataaa gcgacgcaag ttcttctagt tctcgaaaac
     3541 ttcgagccac gcgaacagat cccgtatcaa gaacatgctt aaaatgtgag aggcgctcaa
     3601 gcctcgcaat cgatttgtga tacggctgtt tgctgtaacc gtcaaaaccg agggtaatga
     3661 ttggcttatc gaaaagagcg gcctcgatag cagttgttga gaaaacagaa atgatgagcg
     3721 acgagtaata gagcatactc gtaaaatctt tcttattttc agaattttcg tcatttgtcc
     3781 tccagttgaa aataatgtcc ggtttttgca tcagtttttc atatttcttt ttctccagct
     3841 caatctcgcg aagtcccaca tacggccgga ccataagacg cacgcccggt ccgaacttcc
     3901 ctgcttttat ccatgcatac agttccgcga gaatatcggc ctcatccata gcatacacag
     3961 accctgaaat atacaaaata aaacgctcat ctttattaaa tccaaatttt tcaaaaaatt
     4021 cttcccgcgg ccgcacatat gccctccgtg tgtagtcatc aaactgcggg aaaccaacgg
     4081 gcacaatttg ttctttccta taggcatgat agtcgattgc ctcccgcatc ataggttcgt
     4141 tttgaacaag caggaaatca gcatgcgtag gaatataata cttattcaaa tgatcccaat
     4201 tagcgggcat ggcaaccgtt cgcacttttt gacgtttact ttcagcagta agttcaatgc
     4261 cggggttaaa tgccacattc ggtagaaaaa caagttgcgg atgatatttc gaaaagagat
     4321 acgagtaagg acgcgtatga aaaatacgca tgtaaatcgc cggcacaatt tttcgtttca
     4381 gaagtttaaa tcgtccgaac gtattcgcaa taaatgcttt tacaatccac aaatgacggt
     4441 tacctcctgc gggcggtgca tcagctctcg ctccaaaggt ggcgagaagc gccgtcgtgg
     4501 gcgtaaacac cagatatgag taaaaaaact gaaacgcttt ctcgagaaac gtctccggaa
     4561 aagtcttgga aattttctcc acgacaacat gcggccgtga gctgtatgga gacacgagtg
     4621 cttcaaattc gggaaggttc ggaataagca aaacgacgcg catatcaccc ggtcgcgtca
     4681 gaaggtcaac aacgccgtcc ggcataataa ggttgcgggc ggcgtttatg gcaaacaggt
     4741 ctacaaatat cgttttagtc attggccatg cgtgttcgat agtatcacta aaagtgccct
     4801 attaaaaccg cctgcagcct ccccgcgtta ctttgcaagc aatataaatg ttgtagaatg
     4861 tgacacatgt gcgctgtttc aatacgcaca taatttttcg agaaatgcgt atcgcaatga
     4921 tattcagaaa gctaaatgtc aaagggggca cccagcgcca gggcctttca cttgcccggg
     4981 aactaaaaaa gatggggcat gacatcaaaa tttacacact caacctagac agagaaaagt
     5041 gttatccaga attgttaaat ttattcgaag taaaaacgct caaggacaat gaaaaagacg
     5101 ctccctatta tcctatgttc ttaaccaaaa tcggcgtcac tgaagtgagc aaggaaaaca
     5161 aattggcgca ggatctcgcc gagataatgg acgataattt tgatctatta aatccgcacg
     5221 atcaagtgag ttacaaagtt gcctattact acaaaaaaca gaagaagaat attcctgctg
     5281 tctggaacat gaacgattta ccactcaagc agtggggata tgcccgcatg aagggctgtg
     5341 acgaaagatt ccatcagcca tggtatcgcc accttgctta caaacttttc gatttttacg
     5401 acaaacacaa atttatcaaa catatggata agatcgtggt cgtcgacttc tttaaccgcg
     5461 aacttgtaaa aaaatacctc ggtttgaaag ccgatactgt gcgaagcgga cccgatttcg
     5521 agcatttcat ttttgaggca aaaagccatc ctgggaaaaa aatgaaaata ttgacgagcg
     5581 gcattcttat gcctcatcgg cgatttgaag acactattgc ggcgctccct attcttcaga
     5641 aaagaggcta ctcacccacg ctcaccatca tcggcgacac gaataacgac aagaaatatt
     5701 ttgcaagatt gggcactctc gttgaagaac tcaatttaaa gaatgttgta aaattcaccg
     5761 ggcgcgtttc tgaagaagac ctcgtcaaaa gctatcaaac ccatgacctc tatatttttc
     5821 aacaccattt gcagagtgac gggctatcat cgtttgaagc ggcggcctgc ggcatgccct
     5881 ttattgtttc taaaacagcg ggttgccatg aagtgctgac gcatggtgag aacgcactat
     5941 tgattgaagc caagaaccca gatgatatcg ccgataaagt tgagcaatta attaaagatc
     6001 ctgaacttta tgcgaaactt gcaaaaaatg cccacgcttt tgttcacgag aatttttctt
     6061 ggcccaagta tgcaaaagga gtgctcgata ttatggaatt agcattgcgc aaatatgagt
     6121 gataactact caaacgaagg agatgcgacc cttctctctt tagatcaggc gcacaacttc
     6181 acagactgga tgtacgcgca aatcaagcct tacttgaggg gcgacattct agaaatcgga
     6241 agcggtattg gcacctattc aaaaaagact attcgtgatt tttctcaaag ccgcatatgc
     6301 ctctctgaac tcgaccccgt ttattgtaaa aaactgtctg agcaaaactt tggaaacagc
     6361 aggatcgaaa tacgttcact taatctcgag caagaaacag acttcctacc catttcccaa
     6421 aaattcgact gtgcctttgc tcttaatgtc attgagcatg ttaatgagca cgaactcgcg
     6481 cttaaaaacg tatatagagc gctccgccca ggaggagtct ttatagtgct tgtaccagca
     6541 catcctttgc ttttcaacaa tcttgatcga gccttcggtc attaccgtcg ctacacacgc
     6601 aggggtttta aagaattagt ggcaagaaca gcctttaaaa tcgaacattt gtttatgttc
     6661 aacgccctgt cgatgctcgg ctggtttgta aatggaacac tgtttcgaaa aagcgtactt
     6721 catgacaaga ccctagcatc tttcaataaa cttgttccaa ttatccgccc aattgaaact
     6781 tttattttac agaaaaaaat cggcatatca cttatcgccg ttctcaaaaa atgagagaag
     6841 tttaacttct ttcatttaaa caatgtattc taaattctgt ctagtaattc gagttcggcg
     6901 gtatcagttt ttttataatt cttgatatat tcttttgcaa aattcttata gcttccagca
     6961 ccgccatgta tactactaaa taatccatcc agagtcagga tatttgtacg tcttgtccct
     7021 agataatcac ccaagctcac ataaggattt tcaagaactt tatcaaatga tcgctgaaaa
     7081 gatttttttg cttgtgttag acctcctggt aatagatcaa atccattctt aacctgtacg
     7141 taaacgctta taatccgcat atagctgtct gtgtcgataa tttttgcctt gtaggaactt
     7201 tggaataaag tgccaacagt tttatttttt tcattatagg actttgccag acttgtgcac
     7261 atcttatgca taaatcggct aattccgttc tccgtagatt cctcgagaat taaatggaaa
     7321 tgattttcaa caagggcgta agccagtatc gaaaccaatg atttagccgt atgccatttc
     7381 ggagggaatt gcattggaaa gtttctgcta taagttagac agtccctttt ccaattttga
     7441 ggaacatcag ttctgtttaa ataaaagagg cttcttaaaa aatttttacg atcatggtca
     7501 tcaagcacga taggaagatt tctggtcccc ctttgaatta tatgaacata cgaccctctg
     7561 ccgtaactct taactctcat atttgagcga agtttaactt ccctcaatta tacaatgatt
     7621 gtaaatagtt tcgcaagctg tcgcgccaat acggcagttt tggtagcttc gtatttaaaa
     7681 taacattcga aggtgcgcgg ggaactttaa agactgaatc ccgtttaacc attttgtatg
     7741 ttgccttggc cttcttccca agaaactctg aaatctcatt aataacatcc atagtagtcg
     7801 cttcgcccac ttttatgtcc ggcgcaatat gataaattcc tgattctctt ttagatttaa
     7861 tcaaatcaaa aagtgatttc acaaaatccc ctgtccatgt cggcgagcgc cactgatcta
     7921 ctataacttc aacttccttt tcttcctgga gcttctttat gaactgttcg acgaaagtct
     7981 cccttccctt cccgtaaagc cacccggctc gaacgacata ccacggtgtt ttttgttttt
     8041 ctcctgccat cttcactatc tcttccgctt cagttttcga tctctgatat gcgcttacgg
     8101 gcatcggttt ttcttcctcg gaatatcctt caattttata ctctccttct cttactcgtc
     8161 caaaaacttc agacgaacta atttgtataa aagggcctcg tgctccactt ttttgaaatg
     8221 cataaatcaa atattctact ccgcgagtat ttacatcaaa gcagagcttc tcgtccgctt
     8281 cacatttatc aacactcacg attaccgccg tgttgataat agcgtctggt ttgtatttag
     8341 aaattgcctc gtcagctgaa gtttgattcg taagttcaat atctttatgc gacagcgcaa
     8401 taatctcaaa atcattttgc ttaaaggaag aaatctcttt tcccaaaagt cccaaggctc
     8461 cagtcactag aatgcgcatg gtgaaataat agcccaaaaa atggctctat tccaagcgtt
     8521 tcataattga cactacatat attttgtgct aacattttga cagaacattt gagtgctaag
     8581 gtgcccaagt aagcactttc ccgctcgcca acacacagaa attcccgtta tgaacgaaaa
     8641 agttgtccta gcaatggtca cttattaccc tgatccagag ggaagagaca aaatcaggtt
     8701 tacactggcg ttgaatactg taaggaatgc agttgcggcg gagcacggag ttgtcgtagt
     8761 ggacgactca ccctcactcc ctgtgagcgg accttacgtc tcaaagcgtc ttgccgaggt
     8821 aggcgcaaga gtgtttcctc aattgcatcg cggcctcggc ccggccaagc gccaatcttt
     8881 tttccacgct cttgagcttt gcgtcgctag gtcacatgcc ggcattctgg ccaccgaggc
     8941 ggaaaaagac atggcgcctg atattccgag gatgcttgcg gcgttggaac agcaggactg
     9001 ttccatctgc gttccgagac gcacttccga aacatggcca acgtggccaa cgcaccaaca
     9061 gcgaaccgaa aaagaagcca acgccggatt cgaagcggca acgaatcgca acttcgacgt
     9121 gatgtttggg cctgttttat tcacgctcga ggcagcgtcg cacttcgtca ttcaggaacc
     9181 ggcgaaggtt gggttccccg atcagtatgc acaacatttc gctctcgagc aggccacctt
     9241 tgctggccgt cgcatttgcg agtgcgattc ctcgttcact taccccgccg cacagcgggc
     9301 cgaggaagag ggtgcgctct ccgacgaaat gcggcaaaaa cgcattcgtc aacgtgacac
     9361 gcttatcacc ggttacgagc atgttgctcg ttttatgaag caactggcac aaagctgaag
     9421 aaaggctcct caccaggcaa tccgccacgc ggattgcctg ttttattttg gctagccgaa
     9481 ataaaagaat accgtcatcg aagaaaacag ctagttacca aactcactca ataggtataa
     9541 aatacacaca ctaactttac acgatcgtat aaagttagtg tgtgcgacct cggaccatga
     9601 tcgaccggaa attcgtgact taaatgagaa tttatcgagt agcaaaaatt actgaagcag
     9661 tttaagcagt ctttcggttt cctccccggt taatccaggg ctgtttgaaa agtaaaaacc
     9721 cgagcggtgg agaaaatctg catttggaca gattgttttt tcttttacgt attttttgta
     9781 gaatggctgt ttggtgatgt caccagtgat gatcgggcgg atttcaacgt tggctttttc
     9841 aaatttttct ttatacttct taaaaacttt ctcatctgtg cagataactg gtatcgcgaa
     9901 gcctgaaata aaatccatgt gagacatatt gagcggaagg aaatcaggat ttttctcaat
     9961 cacactctca aatcgtttaa aggacgcctc tcgttttgtg acgattatat cccaatactc
    10021 gagctcgatt tgaccaagat aaccctgcat gtcagtcggg cgcgcattat acgctaaatc
    10081 ataaaaagtg tatttggcat agaaatcatc gactccgtgt tttttacgaa gttctttttg
    10141 cactggttca ggtaaattgc gatcccagcc gtgtgcccga cacatgagaa gtgcgtgata
    10201 aagttccacg tcatctgtgc atatcatccc cccttcaact gtcgacagat gatgggcaac
    10261 aaaaaaagaa aatgttgaag caagccccca attgccgaga agccgacccg acacacgaga
    10321 gcctagtgat tcgcaattat cttcaagcag tgcaatgcct cgcctatcgc agagtgctct
    10381 gatttttttt atatcatcag aaaagccgag cgcatttgta ataaacaaac cgtcgattgc
    10441 attgatttct ttctcgagaa tttgggggga gatattgagt gtttgtagcg agcaatcaag
    10501 cgccacaggt gtgaggccga ggctaatgag cggcataaca ttggttgacc atgtcagcgc
    10561 cgacacaccg atgcggtcac cttttttcca gcggccgaga ttcatcattg tctgcacaag
    10621 aagtaaattt gccgcactac cgcttcctgt cataagggca aagcgtgcac cctgtttttt
    10681 tgcaaaattt aattcaaacg ttttcgtctc cttccccatg ctaagtgtct gacttcgtat
    10741 gacgaaatca gcaagacgcc gctttgtact gtcgttgttg taaaaagttg agtgtacaag
    10801 tctgatcagc tctgcatttc cagatgtaag aaccgtgatc tcttcttgca ccatacgctc
    10861 caccaattcc tcaaaagtga cctctggagt ccagccaagt tcccgctttg ccttggagct
    10921 gtctccaatc acctcatata tttcagttgg ccgataaaat gatttattga ctcgtatacg
    10981 cactttccca tgctggtcaa gcgccgtctc gcgaatagcg cttcctttcc atgtgagctt
    11041 caagccggcg gctttggccg ctgcgctcac aaaatcacgg actgaacgcg cagtgccagt
    11101 cgcgatcaca tagtcatccg gtttatcctg ctgaagcatg agccacatgg cttcaacgta
    11161 gtcgcctgca tagccccaat ctcgtttcgc atcgagattg ccgagctcaa gcacatcatc
    11221 tcctccttca gccattcgtg cgactgctcg tacaattttt ttcgtcacaa attgctctcg
    11281 ccgcttcggt gattcgtggt tgaagagaat tccggaggag atataaaccc cgcgagctct
    11341 cgcgcctacg acgaaatcct catgagcccg caccttcgaa accccgtatg ggctgactgg
    11401 agcgaaaggg gttttctcgt cccgcgctcc ttcccgattg ccgaacattt cactcgtcga
    11461 cgcctggtac agtctcgcct tcgaattaac ccgcaccgtt tcattcacca gccgtgcgag
    11521 gccataataa tttacttcca tcgtttcttc gggtatttca aacgattttt tcacgtcaga
    11581 ttgaccgcct aaattgtata cctcgtccgg ttttgatgtt tctactgctt ttcgcaaagt
    11641 cgcaagatca cgcagatcgc cttcgataaa agttaaactt ttcttctctt ctaaggagaa
    11701 atctctaaaa tagtgcatag ccgccttctc gcttcggcat aggccaaaca cgacatattt
    11761 ttttttgagc aaaaaatgag ttaagtaata tccgtcctgt cccgtaatgc cagttataag
    11821 cgccctcttt tgtcgttttg gcatattagg taagtttgtc ctcaactcca agccgctttg
    11881 catccgcaac gagcattatg cgcactagct ctttaaattt catttttggc ttccagccca
    11941 attgttttcg cgcctttgaa gcgtcgccaa gcagatgatc aacttctgtg ggtcggtaat
    12001 atcgtgggtc gatcttaatg tatttttccc atttcaaaat acccgcaatc ttaaatgcct
    12061 cttcaagaaa atctctgatc gtatgggttt ccccggtggc aatgacgtaa tcgtcaggct
    12121 tatcctgctg aagcatgagc cacatggcct ctacaaattc cggcgcgtat ccccaatcgc
    12181 gcttagcctc aagatttcca agatagatat attttcgttt gcccgccaag atttccgcga
    12241 tgcttatcgt gatttttctc gttacaaaat tttccccacg ccgcggcgac tcgtggttaa
    12301 ataaaattcc gttgcaggca aatatgttat aagcttcacg ataatttaca gtggtccagt
    12361 aggcaaaaac ttttgcagtt ccgtacgggc tccgtggata aaaaggggtt gtctcttttt
    12421 gaggcacctc gcccacacgt ccaaacattt cagacgaaga agcctgataa aatttcgttt
    12481 taatgcgcgt atcgcgaatg gcctcgagta gtcgtaaagt gccgagacca gtgacatcgc
    12541 ctgtatagac tggaacatca aagctgatgc gaacatgact ctgcgctccc aaattataaa
    12601 tctcgtcagg attaattttc tcaataagtc ttgctagatt gctggaatcg gcgagatcac
    12661 catagtgaag aaacacgtga gcctctgcct gtttttgagg gatgatgtag ccaatgcggc
    12721 ccgtatgaaa tgtcgacgag cgccgaagta gtccgtgaac ttcatacccc ttggatacaa
    12781 gaagttcggt gagataggaa ccatcttgac cggtgattcc ggtcacaagg gcttttttcg
    12841 gttttgagta tttttttgct ggcatagatg taactgtggc actatatcgt ttttagtcca
    12901 ttttgaaaag tagtacgcaa caatagaacc tccccagata gcctttcgtt accggcctct
    12961 gtagagcctt tcatactcat caattttatt tttccaatca aacttttgca caaaagaaag
    13021 cgatttttct cccattttct ttctaagcgc gggatctttc aatatttgca aaatagcgtg
    13081 agcgaaacca ctgacgtcag tgtattcgac tagaaaagca ttctcgtgct ccgccacggc
    13141 gtcttccaca ccacagtcgg aagttcccac aagcgccaga ccgtgaagcg ctgcttcaag
    13201 aaaaacaatg ccaaagccct caacatcact gccgaagttc tgggacaata gacaaaataa
    13261 ttcggcattc ttataaaatc cttgaagctc cgcttcttct gcgtctgaaa tcacatggac
    13321 ccgttttgac aaatcgtgtt tttcaataat tttctgcaat tcatcataat aaccgtcttt
    13381 gtattttttt ccgacgataa cataattgag ttccggaaac tcccgagtga cttcggcaaa
    13441 agcttcaatg cttcttttgt atccttttcg ccatcgaatt gcgccgacgg acagaatata
    13501 tggcttatac atgcgtgcac gatgatcgtc tgcaacaggc atatttcttt ccactcctgg
    13561 agtaattact ccaagtgaaa tgcgcggagc gattttttcg acctcttttt taataaacgt
    13621 gctaatggca acacgagcgt acgctccctt aagagcagcg cgcgcaaaaa aacctataaa
    13681 ccgacgccta agaaaatgaa gtgttccggt tcccacgaga gttaagacaa ttttttttcc
    13741 gagtccatta tttacgagcc acgcgagaaa tccataggga taggcgtcaa atgcatgaat
    13801 gacgtcgctc gctctggccg cacggcgcaa cctaaaaagt gtttgaaaaa aactaaaagg
    13861 cgtcattacc aatttctctt cgccgggaag tttcgagaaa agagaagtaa agaggtggac
    13921 cgtggctccg cgcgatcgaa atccgtccac gatatgccgc gagagtacac cacctccatt
    13981 gtcaaggcgc acgttgtgtg tgagatatgt tattcgaagt gatcgcgctg tccgctcgag
    14041 tagttctctc gtttcttttc ctgtgttcgt ccaattgaat ttcttcacct gttcaaagcc
    14101 cgcactgata tggcgcgccc tcgcctcggg attggttaca atagtggtca tagacgccgc
    14161 gatttctgcg gcattatatg ggtcgacaag aaaagcgctc ccgtgagccc cgagttccgc
    14221 cgggccgaaa atattcgagg tgatgactgg cacccctccc gccatcgctt caagtatcgg
    14281 aaaacccgta ccttccacga tgcttggaaa tacaagtccc tcggagcgct tatacacata
    14341 acttaattcg tcttctttta aatgtccgag aaatagcact ttttcattaa ggttatgttc
    14401 ctgcacgaac cgctgcattt cttcgcaata tgctccttct ttttttccgc cgagcacgag
    14461 tgagaatccc actcctgctt cactgtccga gaaaattaga aaagccttta taatatttaa
    14521 aatatttttt cgttctttga tcgttccagc aaaaaagaaa aaacgagacg gggtgacgac
    14581 ctttttttcg ggcaatttgc agacgtcttt gtatccgtga tagacgacag aaattttatc
    14641 gcttgggatg ttataaaaac gcattgtgtc gttttttgtg gaatgggaaa ccgcaataat
    14701 atgttcagcc cgcttgagtg agcggtgatg ataccatcga actaaaattc ttcgcagact
    14761 gtcttttatc gatgtcgctt ttaaatagtt gtatggataa tcaagggcga tgacgatcga
    14821 acgtttaggt ttgaaccaaa aaggcaaaac tggggtattg aacaaatata catcgcaggg
    14881 aacttttttt attcggtcga gccagaatcg tccatttccg agcgcttccg tcttccaatt
    14941 ttcacccggc aaattg
//
LOCUS       DATDSU010000105        11842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_934088, whole genome shotgun sequence.
ACCESSION   DATDSU010000105 DATDSU010000000
VERSION     DATDSU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934088"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VI981_03600"
     CDS             complement(<1..425)
                     /locus_tag="VI981_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479411.1"
                     /translation="MLLVALGSAAVSAQEGGFALPNSYNKLPLSIELAPEFPGPFEAV
                     ILKAVSFSFDLDRSTLTWRVNGTIVKRGVGEKTAEIKTGNVGSETSVTLEAVTDRGTQ
                     STSIVIRPGEVGLSWEALSYVPPFYKGKALATFESEVRV"
     gene            complement(532..1773)
                     /locus_tag="VI981_03605"
     CDS             complement(532..1773)
                     /locus_tag="VI981_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479412.1"
                     /translation="MASQSTAKILWEARKELTPLFKKAKQKELNEEELQSLIHNKPEK
                     THFPFFMLFFAIVKDALDAILTAAAIPSGGVTEVVLIIVSAICAVILFIWFLYRIGFK
                     QGATRQQAKGLAKKLTFQVRRQGKGFGWKVGGTIVGENVPVLNVIPFNTILVLWTYFS
                     EKKIVRLFLQSKQILEKRGLQIESRIRGATSDINRSGGIQTDVDGISPPPIPDDMLER
                     GQGGARAMPPQRPPLLPQSYTDRSLKPPPLPQSYLDRAHKPPPIPNRHQTRERAAEGA
                     VPPQMPPPLPHEKPPKLQAGMPHRRQKEEPQEQGDEDEESDTDWSAILDELEDEYLET
                     ENEEVFQEMLNDKALEHLLREMPEGFSDEFSGEVSEDGFLIDLDGNETEVRAIDCIHP
                     DDLDEIKVRTLHDFLELRDKK"
     gene            complement(1882..3693)
                     /locus_tag="VI981_03610"
     CDS             complement(1882..3693)
                     /locus_tag="VI981_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEY4479413.1"
                     /translation="MSFLSSLTSKREKVPQQITSILPHEIYEAGVLELKDIIAPSALR
                     ISPKELNLGEKVVRTFFVMSYPRFLSEGWFSPIINLDKVFDISIFIHPIETSKVLRQF
                     QKKVAEVQSQIHTREEKGLVRDPMLDTAYQDLENLRDALQQAQEKLFDVVVYISLYGE
                     NDAELDKVESEVKSILEAKLVYIRPAVFQQEQGFRSTLPLANDEIQVHSKLNSSPLSS
                     LFPFVSFDLTSDKGILYGINRHNSGLVLFDRFSLENYNSITFAKSGSGKSYCTKLEIL
                     RTLMFDTEVIVIDPEREYEYMAQASGGKYFNISLNSPHHINPFDLPVPREGESTADVL
                     RSNIINLVGIFRIMMGGLTPEEDAIIDRAITETYALKDITAESDFSNIEPPLMSDFEL
                     VLAGMQGGESLVQRLSKYTRGTWAGFINRPTNVDINNKFIVFSLRDMEDELKAVAMYI
                     VMHYIWNAVRKNLKKRLLVIDEAWWMMKSEDTASFLLSLAKRGRKYFLGLATITQDVD
                     DFLKSPYGLPIITNSSIQILLKQSPTSIDRVQTTFNLTDEEKYLLLESDVGEGLFFVG
                     LKHVAIKVIASYTEDQIITSDPSQILAIKKAKEEMGS"
     gene            complement(3759..4136)
                     /locus_tag="VI981_03615"
     CDS             complement(3759..4136)
                     /locus_tag="VI981_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEY4479414.1"
                     /translation="MSANDANEKIIYRDLSYKLTGLFFDVHNEIGRFEKEKKYADAIE
                     KKLLEKNIAHKREFCIDSLGNTVDFLIEEKLILEVKAKQLIVKNDFYQTQRYLQSSQM
                     KLGLIVNFRNRYLKPIRIIRINR"
     gene            complement(4159..4797)
                     /locus_tag="VI981_03620"
     CDS             complement(4159..4797)
                     /locus_tag="VI981_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479415.1"
                     /translation="MVTSALSSQKFVPIEEIRDGTVILKDGSLRGILMTSSLNFALKS
                     EDNQKSIIYQFQNFLNSLDFSTQIYVQSRKLDIRPYIALLEDRYREQRGELLKIQTRE
                     YIEFIKSFTETTSIMTKSFFVVVPFTPSAMGEKKNGISSMLFGRKGKTKAQEKQDSFE
                     EHKSQLLQRISVIEQGLLRTGIRVVALGTEEIVELYYKIFNPGATEKPLQLS"
     gene            complement(4846..5289)
                     /locus_tag="VI981_03625"
     CDS             complement(4846..5289)
                     /locus_tag="VI981_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEY4479416.1"
                     /translation="MRFQVPQFIGVEDKIIGPLTLKQFIYLAGGGALCFMLFQFTPKI
                     LAFPLMVPVAVLTAALAFYRFNNKPFISVVESAFWYVFGGKLYIWKKEDKKIIPKTVV
                     KKTDEPLMYVPKLSDSKLKDLTWSLDINESLNPVTHDAKHPPHSS"
     gene            complement(5309..5665)
                     /locus_tag="VI981_03630"
                     /pseudo
     CDS             complement(5309..5665)
                     /locus_tag="VI981_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(5753..6301)
                     /locus_tag="VI981_03635"
     CDS             complement(5753..6301)
                     /locus_tag="VI981_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479417.1"
                     /translation="MEYSQETINEKIASLPSDVKDAINSVDIGMRVQTIGSKYHLQID
                     ELNELEKTTAYVMLGLCHPSSYIEVLKTKLAISNETAEAIALDINETIFKPLRASLRK
                     MHSLSEQEEILSAAPQKADATAPKVQSFMGPYSSSTPAAPLNPPATPQKTTDTPIPAS
                     KPEVMPPTPNMPKYADPYREPV"
     gene            complement(6340..7125)
                     /locus_tag="VI981_03640"
     CDS             complement(6340..7125)
                     /locus_tag="VI981_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479418.1"
                     /translation="MDFNPQQIKHRLIKLPPVLQDALISSDVIEKIVAVAIDNDIPQS
                     SAEALISEVMWILLGLKTTRDLAKNIENKVGVAKETAQKICEDLYLYVFKPLEEPLRM
                     IHNLGRLPFFGGPVTLPDLKPAQITPAKPLIGYIGPYTTDTTPSAGAKLPTAPTSRQS
                     ESVPVGGQTFTPFTPSPSQPRETPLYNALSANREFTPHSEVHKETGTLSSDAPNSKIP
                     EIRPNTLPSLIEQKLRGSISMQKEDKEVPPPQSIIKNDESKNN"
     gene            complement(7244..9238)
                     /locus_tag="VI981_03645"
     CDS             complement(7244..9238)
                     /locus_tag="VI981_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479419.1"
                     /translation="MKPLYKKIILSIFVVGVLLSFAAIANAQKTALNLLSEAPIIGPF
                     ARAASIASPLLNKLADQGGFFKWLDPDQWFPAVITLAGTYIIAAVSVFTGICGTFFNF
                     AINLNIVRMTGIVNSLDFITDSWTFFRDALNLFFIFSLLYIAITTILGQENGGTKKLL
                     GNLIIAALLINFSLFITKAVIDVSNIASFGFAKQLPEFGYGDNVAALAYETTNTGPAG
                     SVMDALKLTTIYDAREALADDFAEIAATTPKLVTAVIMSSIFLIGLAFLYLTAAILFT
                     IRFVLLILLMVTSPFMFAGMVLPALSGISEKWWSTFWHQIFFAPAFMMLFMLALKFVT
                     SPGFNKIVNTSDASFMAFFADAGNGTISIVFNYFIAMAMLISAVAAAKSLGATGAEFA
                     GKVGGKLSFGLAAGLARSTVGRMSYQYTKNKARRDWATESWVGEQAMKVNRFLSERSF
                     DLRNIAGAAKPYVGEPHEGGYRGSIKKSDEEKKRLIQRMTGTSTKHIARQLKFAKRKL
                     AWTGIEEYKDPATGETKLRGVPWKNLLNFMLGRSRLEFSGYSEHIADIMKTYNKRAVT
                     SLREDLKKLRNLLEKPLVPALDAAGNPILDADGNPKVTENFSVIQRGRTLANPLASST
                     DDDANDMKDVVFKTKKEVEEQIEKLTNRIGQINEEVIGED"
     gene            complement(9248..10210)
                     /locus_tag="VI981_03650"
     CDS             complement(9248..10210)
                     /locus_tag="VI981_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479420.1"
                     /translation="MLWHESLFTLTNKKILAIFWLLVFVATLFFIGPLKSSAQLFGAC
                     NKITVPPVPQGPVVPVYDSNVARSTANINRSTTNQENKECLFDGWAKFAARSVINQLT
                     TSLIQWIRTGFEGSPTFIQDPVGFFGNIADEELGRLLDSSELGFLCDPFLLQIKFGLW
                     ARSNRYKYPSRCTITGAVNNIANFTSGTAGSFKDGGWDGWFRMVNTPQNNPYGGYIYA
                     NELYNIRVAGRQNSVLNKLNWGNGFHSLEKCTRYVDTDDNPSTPDECAQFEIQTPGSL
                     VNDQLQQWTGQDLEYLNLAKEFDDIVLALVELGVSQVMQNGVAR"
     gene            complement(10229..11221)
                     /locus_tag="VI981_03655"
     CDS             complement(10229..11221)
                     /locus_tag="VI981_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479421.1"
                     /translation="MLTRIKLPSKKIMMIAAAALFLIIVIIYANREKQALKNPNEGLL
                     ASEKKLESKIVELDTDQDGLKDWEEILWGTNPSLADTDGDKTEDGDEVEANRNPARAA
                     PNDSLDAATTTLPATLTAPAIKLNTTERIGRGLWERYLAIKLSGGELSADDRVALIQS
                     VVQEEEGMPIGKSYTRTDIKIIPQSDNETLKRYAEAMGAVLERNQSQESELEIINRYT
                     LDETKTAELEKLGTIIALYENVIAEALKIEVPQPLLEFHLSFLNISVAIKTSIMGMQK
                     IASDPIIALDAMGNYNSATEQFVAIMQDLNRYYTTFLVEFNENSPGYSFTHLYE"
     gene            11401..>11842
                     /locus_tag="VI981_03660"
     CDS             11401..>11842
                     /locus_tag="VI981_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY4479422.1"
                     /translation="MTCPEVSSSYALSAVQTFRTLLFIENLSEIKNVSRSTSGYNYPM
                     KRGRSSSLCVAVSTILLTFVFCVGQVAFAQSYFFRTLKLGASGADVRELQKILNSDPD
                     TVIAKSGPGSPSNESTYFGALTKGAVMRFQQKYKNDVLIPAGLTA"
BASE COUNT         2984 a         2533 c         2730 g         3595 t
ORIGIN      
        1 atgactctca cctcgctttc gaacgtggca agcgccttgc ctttgtagaa aggaggaacg
       61 taggaaagcg cttcccacga aagtccgact tcacctgggc ggatgacaat agaagttgac
      121 tgggttccac ggtcggtcac ggcttctaaa gtgacgcttg tttcagaacc cacatttccc
      181 gttttgattt ctgcggtttt ttctcccaca cctcttttta ctatagtgcc gttgacacgc
      241 catgtgagtg tacttcgatc gaggtcaaac gagaaactga cagcttttaa gatgacggct
      301 tcaaatggcc ccggaaattc cggtgcgagt tcaatcgaca ggggtaactt attatatgaa
      361 tttggcagtg caaaacctcc ttcttgtgcg gaaactgctg cggagcctaa agcaaccagc
      421 aacatacaaa aaaccgaaat ggcaattaac cgccgcatag gccctaattt tatcatgcgg
      481 aaaagagaat agctgttgat accctttgag agaggtagta agtatgcgta tctatttttt
      541 gtctcgtagt tccaggaagt cgtggagtgt ccgaaccttt atttcatcta ggtcgtcagg
      601 gtgaatgcaa tctatcgccc gcacctctgt ttcgttgccg tcgaggtcta ttagaaagcc
      661 gtcctccgaa acctctccgg aaaattcgtc ggagaatcct tcgggcattt cccgcaacaa
      721 gtgctcaagt gctttatcat tcagcatttc ctgaaatact tcctcatttt cggtttcaag
      781 atattcatct tcaagttcat ccaaaattgc cgaccagtca gtatcggatt cttcgtcctc
      841 gtccccttgc tcttgaggtt cttctttctg tctcctgtgt ggcattccgg cttgcagttt
      901 aggcggtttt tcgtgcggta atggaggagg catttgcggt ggcacagctc cctcggccgc
      961 cctttctctt gtctgatgac ggtttggaat tgggggcggt ttatgagctc tatcgaggta
     1021 actctgcggt aggggtggcg gtttcagaga tctgtcggta taactttgcg gcaagagcgg
     1081 cggcctttgc ggtggcatgg cacgtgcccc tccctgccct cgctccaaca tgtcatccgg
     1141 aattgggggt ggggatattc cgtcaacgtc cgtttgtatt ccgccgcttc ggttaatgtc
     1201 gctggtagct ccccgtatcc ggctctctat ttggagccct cttttttcaa gtatttgttt
     1261 tgattgcaaa aagagtctca ctattttttt ctctgaaaaa taggtccata aaacgagtat
     1321 tgtattaaac gggataacgt tgagcactgg gacgttttca ccaacaattg tgccacctac
     1381 cttccagccg aaaccctttc cctgtcgcct gacttggaaa gtcagttttt ttgccagacc
     1441 tttcgcctgt tggcgcgtcg ctccttgctt aaaccctata cgatacaaaa accagataaa
     1501 taagatgact gcgcaaatgg cgctcacaat tatgagaaca acttccgtga caccaccgct
     1561 cgggatcgct gccgctgtca atattgcgtc taaggcgtct ttgacaattg caaagaaaag
     1621 catgaaaaaa gggaaatgtg ttttttcagg cttattatga atgagcgact gaagctcttc
     1681 ttcgttcagt tctttttgtt tggctttttt aaaaagaggc gtgagttctt tccgtgcttc
     1741 ccataatatt ttggctgttg attgtgaggc catgatgcaa tactaatatg ccaatgagta
     1801 ctaatatact aatggatacg aatggggttg ttcattagta ttattcgtat attagtaaca
     1861 attagtatat ttgcattagc gttagctccc catttcctcc ttggcttttt ttatggcgag
     1921 aatttgggac gggtcggatg tgataatctg atcttccgtg tatgaggcaa tgactttgat
     1981 ggcgacatgt ttcaatccaa caaagaataa gccttcgccg acatcagatt caagcaaaag
     2041 gtacttttcc tcgtctgtca agttgaatgt ggtctgaacc cgatcaattg acgtcggcga
     2101 ttgcttgagc agtatttgta ttgaagagtt ggtaatgatg ggcagaccgt agggagattt
     2161 caaaaagtcg tcaacgtctt gagtgatcgt cgctaggccc aaaaaatatt ttcttccacg
     2221 ctttgcaaga gaaagtagaa atgaggccgt atcttccgat ttcatcatcc accacgcctc
     2281 atcgatcaca agcaggcgtt tcttgagatt ttttctcact gcgttccata tataatgcat
     2341 gacgatgtac atggcgactg ccttaagttc atcctccata tcacggagtg aaaagacgat
     2401 aaacttatta ttgatatcta cattcgtcgg acggtttata aacccagccc atgttccacg
     2461 agtatatttt gacaacctct gtacgagcga ttcaccaccc tgcataccgg ctaggacaag
     2521 ttcgaagtca gacataagtg gcggttcgat atttgaaaaa tctgactccg cagtaatatc
     2581 tttaagcgca taggtttctg tgattgccct atcgataatg gcgtcttctt ctggagtgag
     2641 tccgcccatc atgattctga agattccgac taagttgatt atgttcgatc gaagcacatc
     2701 agccgttgat tcgccttccc gaggcacggg caaatcaaaa gggttgatgt ggtgaggcga
     2761 gttgagagaa atattgaaat actttcctcc gctcgcttga gccatatatt catactcgcg
     2821 ctccggatca atcacgatca cttctgtgtc gaacataagt gtccgcagta tctcaagttt
     2881 agtgcaatag gattttcctg aacctgattt tgcaaaagta attgagttgt agttttctag
     2941 tgaaaatcga tcaaagagga caaggccgga gttgtgacga ttgatgccgt agagaatgcc
     3001 tttgtcagag gtcaagtcga aagagacaaa aggaaaaagg ctggaaagcg gcgaagagtt
     3061 gagttttgaa tgcacctgta tttcatcgtt tgccaaagga agtgtgcttc gaaagccctg
     3121 ctcctgctga aatactgcag ggcggatata cacgagtttt gcctcaagaa tcgattttac
     3181 ttcagattcg accttatcga gctcggcgtc gttttctccg tatagtgaaa tatatactac
     3241 gacgtcgaac aatttttcct gggcctgctg gagtgcgtcg cgcaaatttt cgaggtcctg
     3301 atatgcagta tcaagcatcg gatctcgcac cagccctttc tcttctctgg tatggatctg
     3361 gctctggact tcggcgactt ttttttgaaa ttgtcttaag acctttgacg tttcaatcgg
     3421 atgaataaaa attgaaatat caaaaacctt gtccaagttt atgattggtg aaaaccagcc
     3481 ctcggaaaga aagcgcggat acgacataac aaaaaaagtc ctgaccactt tttcaccgag
     3541 gttgagttct tttggagaaa tacgaagggc agaaggcgca ataatgtctt tgagttcaag
     3601 cacgcccgct tcatagatct catgtggcaa gattgatgtt atctgctggg gaactttctc
     3661 cctttttgat gtaagtgaac taagaaaact catgggttac attatatcat caggtatcga
     3721 tacgaatatg cgaatcattc gaatgaaacg aatatatgct aacggttaat tctgatgatt
     3781 cttatgggct ttaggtaacg gttcctgaag ttaacgatga gtccgagttt catttgcgag
     3841 gattggagat atcgttgtgt ttggtagaaa tcatttttga ctattagttg tttggcttta
     3901 acttctagta tcaacttttc ttcgatcaaa aaatctacag tgtttcctaa ggaatctata
     3961 caaaattctc ttttatgagc aatatttttt tcgagaagct tcttctcgat ggcatctgca
     4021 tatttctttt ccttttcaaa tcttccaatc tcgttatgta cgtcaaaaaa caatccggtt
     4081 agtttatatg aaagatctcg ataaataatt ttttcattcg catcattcgc actcattcgt
     4141 atattggcat cgatacagtt agcttaactg aagtggtttt tcggtagctc cagggttgaa
     4201 aatcttgtaa tacagttcca ctatttcctc tgtaccgagt gcgaccactc taatgcccgt
     4261 tcggagcaag ccttgttcga tgacagaaat gcgttgcagt agttgagact tgtgttcttc
     4321 gaatgagtct tgtttttcct gcgccttcgt tttgcctttt cggccgaaaa gcatactgct
     4381 aatgccgttt tttttctctc ccatggcgga gggtgtaaac ggcactacga caaagaagct
     4441 ttttgtcata atgctcgttg tttcggtgaa acttttgatg aactcaatat attcgcgggt
     4501 ttggatctta agcaactcgc cgcgctgttc tcggtatcgg tcttcaagga gcgcaatata
     4561 gggtcgaata tccaattttc tcgattgaac gtaaatttgt gtcgaaaagt cgagcgagtt
     4621 taaaaaattc tggaactgat aaatgatgga tttttggttg tcctcggatt tcagggcaaa
     4681 gttgagcgat gaggtcatga gaataccgcg aagtgacccg tcttttaaaa taaccgtacc
     4741 gtctctaatc tcctcgattg gaacaaattt ttgcgaagat aatgcgctcg ttaccatgag
     4801 atgtggattg taaaattgta aaatgcctct ttatacattg gtgtgttatg agctgtgagg
     4861 tgggtgtttc gcgtcatggg tcacagggtt cagcgattca tttatgtcaa ggctccaggt
     4921 taggtctttg agcttgctat cagagagctt gggtacatac atgagcggct catcagtctt
     4981 tttaaccaca gtctttggaa ttattttctt atcttctttt ttccaaatat ataatttgcc
     5041 tccaaaaaca taccaaaaag cgctctcaac aaccgatatg aacggtttgt tgttaaaacg
     5101 atagaaggcc agtgcggcgg tgagtacggc gacaggaacc ataagcggga aagccaaaat
     5161 tttcggtgta aattgaaaca gcataaagca caaagcacca cctcctgcaa gataaataaa
     5221 ctgcttaaga gttagtggac cgatgatctt atcttctaca ccaataaatt gaggaacttg
     5281 gaatctcata taaagaagcg tataggggtt agcgtttagc gtttagtttt ctaatcatgc
     5341 tcattaacat tttttggatt tcttcaacaa gtggtaataa tttatttgtt tctcgtttat
     5401 ataattgatt acagagcaat agttgtgttt ccaattctgc taccgaacca tccgcaattg
     5461 cttaaaattg cacatactgc ccgagaccct gccttctgta cccttccgcg atatttgagg
     5521 gaacagaaat tgcgcatctt cgcatctggc ttattaaatt gaaccgctca tcttctggca
     5581 gttgtttagt aaaaatatat acttcactaa cgagtgtcat tgctttttgc caaacaacca
     5641 gttttttata tgattcgaca tacattttat catttttaaa ccctaagcgt taatctctat
     5701 aggctattta tttttccttc tatacgctat accctaaacc ctgcacgcta gttcacaccg
     5761 gttctctgta cgggtcggca tatttaggca tatttggagt tggcggcatg acttcgggtt
     5821 ttgaggccgg gatgggagtg tctgttgttt tttgcggagt tgccggagga ttcaaaggtg
     5881 cagccggagt cgaactcgag tagggcccca taaatgattg aactttcgga gccgtcgcat
     5941 cggctttttg gggagctgcg ctaagaattt cctcttgttc actaagggaa tgcattttcc
     6001 gtaaagaggc acgcaatggt ttaaaaatcg tttcatttat atcgagtgcg atagcctcgg
     6061 ccgtttcatt agaaattgcc aattttgttt ttagtacttc aatataagat gaaggatggc
     6121 aaaggcccag cataacgtag gccgtcgttt tctcgagttc gtttagttca tcaatttgaa
     6181 gatgatattt tgacccaatg gtttggactc tcatgccgat atcaaccgaa tttatggcgt
     6241 cttttacgtc gctcggtaag gaagcgattt tttcatttat tgtttcttgg gagtactcca
     6301 tattaagaag cgtttagggt atagcgtata gggtttagtc taattatttt ttgactcatc
     6361 atttttaatt atcgattgcg gtggtggaac ttctttatct tctttttgca tggaaatcga
     6421 gcctcttaat ttttgttcaa taagagatgg aagtgtattt ggccttattt cgggtatctt
     6481 gctattggga gcatcggaag aaagtgttcc tgtctctttg tgcacttcgg agtgaggcgt
     6541 aaattctctg ttggcgctta aggcgttata tagaggcgtt tctcggggtt gcgacggaga
     6601 tggtgtgaat ggggtgaacg tttggccgcc aactggcacg ctttcgcttt gccgcgacgt
     6661 tggtgctgta gggagcttag ctcctgcgga aggggtggtg tcggtagtgt acgggcctat
     6721 gtagccaatg agtggttttg ccggggtaat ttgtgctggc ttcaagtctg gcaaagtgac
     6781 tggtcccccg aaaaagggta acctacccaa gttgtggatc attcggagcg gttcttcgag
     6841 gggtttgaag acgtagagat ataaatcttc gcatattttt tgcgccgtct ctttcgcaac
     6901 accgactttg ttttcaatat tttttgccag atctcgagtg gttttgagac caagcagtat
     6961 ccacataact tcgcttatca acgcttcggc actagattgg gggatgtcgt tatcaattgc
     7021 aacggccaca attttttcta tcacgtcact tgagataagt gcgtcctgaa gcacaggagg
     7081 tagtttgata agtcggtgtt taatttgttg tgggttgaag tccatacgta tagattatag
     7141 ctgtgagcct tttggttggt gtttgagaat ccgaacggtt tctttttcaa ataacatttg
     7201 tttttaattt ctatacccta gaagccaaca gctcaaaact gttctaatct tctccaataa
     7261 cttcttcatt aatctggccg atacgattag taagtttttc gatttgctct tcaacttctt
     7321 tcttagtctt aaataccacg tccttcatgt cgtttgcgtc atcgtcggtc gagctcgcaa
     7381 gcggatttgc aagtgtccgt cctctctgta taactgaaaa gttttcggtt actttcggat
     7441 ttccatccgc atctagaatt gggttgccgg cggcgtcaag ggcgggcaca agtggttttt
     7501 ccagaagatt ccgcagtttt ttgagatcct ctcgaagtga agtaactgct cgtttgttgt
     7561 aggttttcat tatatccgct atgtgctcgc tatacccgga aaactcgagt ctgcttctgc
     7621 cgagcataaa gttgagcagg ttcttccatg gtacgccacg aagtttggtt tcgccggtag
     7681 caggatcttt gtactcttcg ataccagtcc acgccagctt tcgctttgca aatttaagct
     7741 ggcgagcgat gtgttttgtc gacgttccag tcatcctctg tatgagccgc ttcttttcct
     7801 cgtcagactt tttgatcgag cctcgatagc cgccttcatg gggttctccg acatagggct
     7861 tggctgcccc ggcaatattt ctcaaatcaa atgaacgctc ggacaaaaag cgattaacct
     7921 tcatagcttg ctcgccgacc caactctcgg tagcccagtc tcgcctagcc ttattttttg
     7981 tatattgata ggacattctg ccaacggtac ttcgggcgag cccggccgca agtccaaagc
     8041 tcaatttacc gcctacctta cctgcaaatt cagcaccggt tgcccccaga gattttgcag
     8101 cggcaacggc cgagataagc attgccatgg ctatgaagta gttgaagacg atagaaatgg
     8161 tcccatttcc tgcgtcggcg aagaaggcca tgaaggaagc gtcacttgta tttacaatct
     8221 tattaaaacc cggagaagtg acgaatttaa gtgcaagcat gaagagcatc ataaatgcgg
     8281 gagcgaagaa aatctgatgc caaaaagtac tccaccactt ttctgatatg ccagaaaggg
     8341 caggtaaaac catacccgca aacataaatg gtgaagtaac catgagtaag atgagaagca
     8401 caaatcgaat ggtaaagagt attgcggccg taaggtacaa gaatgccaaa cctataagga
     8461 atatactgct cataatcacc gcggttacaa gttttggagt ggtagctgct atttcggcaa
     8521 agtcatctgc cagtgcttca cgggcgtcat atattgtcgt tagtttgagt gcatccatga
     8581 cggagcctgc gggcccggta tttgtagttt cgtaggctag tgccgcaacg ttgtcgccat
     8641 agccaaattc aggtaactgt ttggcaaaac caaatgaagc aatgtttgaa acatcgatga
     8701 cggctttcgt aataaaaaga ctgaaattta taagtaaggc ggcaattatc aaattaccga
     8761 gcaatttttt agttccgccg ttttcctgcc ctaaaattgt ggttatggca atgtagagta
     8821 gagaaaaaat aaagaatagg ttaagagcgt ctctaaagaa agtccaacta tcagtgataa
     8881 aatccagtga gttaacgatt cctgtcatgc ggacaatatt gaggtttatc gcaaagttga
     8941 aaaacgttcc gcatattccg gtaaatacgg aaacggccgc gatgatatag gtgccagcaa
     9001 gggttattac tgcaggaaac cactggtcag ggtcaagcca tttaaagaaa ccaccttgat
     9061 cagcgagctt gttcaataac ggggaagcaa tggacgcagc tcgagcaaat gggccgataa
     9121 tgggagcctc gctaagtagg ttgagtgccg ttttttgagc gttggcgatc gcggcaaatg
     9181 aaagcaacac gcctacgaca aagatcgaga gaataatttt tttatacagt ggtttcatct
     9241 ggtttcatca tcgtgctact ccattttgca tgacttgact gacaccaagt tcgaccaaag
     9301 ccaaaacaat gtcgtcaaat tcttttgcca aatttagata ctcaagatcc tgccctgtcc
     9361 actgttggag ctggtcgttt acgagtgagc ctggggtttg aatttcaaat tgggcgcact
     9421 catctggcgt gcttgggttg tcgtctgtgt ccacgtatcg cgtgcatttt tcgagcgaat
     9481 gaaacccgtt tccccagttt agtttattaa gtactgaatt ttgccgaccc gcgactcgaa
     9541 tattgtatag ttcgtttgcg taaatatatc cgccgtacgg attattttga ggtgtgttta
     9601 ccatgcggaa ccatccgtcc caaccgccat ccttgaaact gccagccgtg ccgcttgtaa
     9661 agtttgcaat attattgacg gcgcccgtga tggtgcaccg agacggatac ttatagcgat
     9721 tgcttcttgc ccaaagcccg aattttatct ggagtaaaaa aggatcgcag agaaaaccga
     9781 gttccgaaga atcgagcaaa cggccaagtt cttcgtcagc aatgttccca aaaaaaccga
     9841 ctgggtcttg gatgaaggtc ggactaccct caaatccggt ccggatccac tgaataagcg
     9901 atgtggtgag ctggtttatg acactgcgag ccgcgaactt tgcccaaccg tcaaaaagac
     9961 attctttgtt ttcctgatta gttgtcgatc ggttgatgtt tgccgtgctt cgcgcgacgt
    10021 tcgaatcata taccgggaca acaggcccct gcggtacggg cggaaccgtg attttattgc
    10081 acgctccgaa aagttgggcg ctacttttta agggccctat gaaaaataat gttgcaacaa
    10141 aaaccagaag ccaaaaaata gccagtatct ttttgttcgt aagcgtaaaa aggctttcgt
    10201 gccaaagcat aataccccta attttaaact actcgtatag gtgagtgaag gaataaccgg
    10261 gtgaattctc gttgaactcg acaagaaaag tggtgtagta acggttgaga tcctgcatga
    10321 ttgccacaaa ttgttcggta gcactgttgt agtttcccat ggcatcgagt gcgataatcg
    10381 ggtcgcttgc tattttttgc atccccataa tgcttgtttt tatagctacg gaaatattaa
    10441 ggaatgaaag atgaaactca agcaatggct ggggcacctc gattttgagc gcttcggcga
    10501 tcacgttctc gtacaaagct ataatagtgc cgagcttttc aagttctgcc gtctttgttt
    10561 catctaatgt gtatcggttg atgatctcaa gttctgattc ctggctctga tttctttcaa
    10621 gtactgctcc catggcctct gcgtagcgct taagtgtctc gttgtctgat tgcggaataa
    10681 tttttatgtc cgttcttgtg tacgatttac cgatcggcat cccctcttct tcctggacca
    10741 cactctgaat gagcgcaacc ctgtcgtcgg cagacaattc gccgcccgat agcttgatcg
    10801 ccaaataccg ctcccagagt ccgcgcccga ttctctctgt cgtattcaac ttgattgcag
    10861 gcgcggttag agtagccggc agagtcgtag tcgcggcatc tagcgaatca tttggggcgg
    10921 cacgagcagg attgcggttt gcctcgactt cgtctccgtc ttctgttttg tcgccgtcag
    10981 tgtcggcgag tgagggatta gtcccccaga gaatttcttc ccagtctttt agtccgtctt
    11041 ggtcggtgtc aagttcaacg attttcgatt ccagcttttt ttcgctcgct aagagccctt
    11101 cgtttggatt tttaagcgct tgtttttcgc ggttggcgta tataatgacg ataatgagaa
    11161 agagagccgc cgcggctatc atcattattt tttttgatgg caatttaata cgggtaagca
    11221 tgttcaagcg cccattatat cacgctgttt aaaggttcga ccttaaatcg gtaaaccccg
    11281 ttagaagcag acacgggtca ctgaaccttg tgttcgctaa aaccaaattg cgttctgctt
    11341 ctaacggggt aaaaaggtcg aaccttggga attcaacttc ccgaatttaa aaatttcacc
    11401 atgacgtgcc ccgaagtgag ctcaagctat gcattgtcag ctgttcaaac gtttagaaca
    11461 ttgttgttta tcgaaaattt gagcgagata aaaaatgtat caagatctac ttcggggtat
    11521 aattatccaa tgaaacgtgg acgatctagt tctttgtgtg tggcggtaag cacgatactc
    11581 cttacctttg ttttctgtgt cgggcaagta gcgtttgcgc agtcttattt ttttagaacc
    11641 ttgaaactcg gcgcctcggg agctgatgtc agggaattgc agaaaatttt aaatagtgac
    11701 ccggatacgg tgatcgcaaa atcgggaccc ggctccccga gcaatgaaag cacttatttc
    11761 ggtgcgctca caaagggtgc agtcatgcgt tttcagcaga aatacaaaaa cgacgtgctt
    11821 attcctgcgg gcctcactgc gc
//
LOCUS       DATDSU010000106         3282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1620041, whole genome shotgun sequence.
ACCESSION   DATDSU010000106 DATDSU010000000
VERSION     DATDSU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620041"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..602)
                     /locus_tag="VI981_03665"
     CDS             complement(303..602)
                     /locus_tag="VI981_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479423.1"
                     /translation="MKKSADKNQLENIVNKISIQIEKLALSTASGFEDVQKQFGILDN
                     RIGGIEIGIAKGNQKIDGLANRLDDFSNTRVKIDIHENLAKRVERIESKLGIKHG"
     gene            complement(632..2425)
                     /locus_tag="VI981_03670"
     CDS             complement(632..2425)
                     /locus_tag="VI981_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4479424.1"
                     /translation="MRSKAESGIKYPKVGLSDVFWHYWQSAKLNSVSLFLTLFGIISA
                     NILEIIVPTFYKKFFDVFEMPGTTSEIGDKLLHIIVMIAVMHGVVWLSYRIATLSNNN
                     FTSSALANIRQRAFDYMIGHSYSFFSNNFVGSLTQRLSRYARAFERISDRIIWNVLPL
                     LVRVTGIIIVMWYEMPPIAILVLVWLVVFVCFNYLFSSWKLKYDIRVAEIDSQTSGLL
                     ADDLTNHTTIQLFTGKVREFFRFRDQTLTQAKASRFTWNLDAAMEAVQSFLMFAIEFF
                     VFYFSIKYWRSGLISVGTFVLFQTYVIGMFGRLWDFTRIIRDAYQGYADAKEMVEIME
                     LPYQIADTEGARELSVREGKIEFKSLSFSFNKMRQVLNGIDLAIHPGEKIALVGPSGA
                     GKSTLVRLVLRFYDPEGGVILVDGQDITKVTQESLRANIALVPQDPILFHRTLMENIR
                     YGRPDATDEEVLDAAKKAHCHEFIDSLPLRYETYVGERGIKLSGGERQRVAIARAILK
                     NPPILILDEATSSLDSHSEAYIQDGFDTVMKGKTTIVIAHRLSTIRKMDRIIVLHDGK
                     IVEEGTHKSLTEREGSLYRKLWELQAGGFIS"
     gene            complement(2628..2822)
                     /locus_tag="VI981_03675"
     CDS             complement(2628..2822)
                     /locus_tag="VI981_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479425.1"
                     /translation="MPSAEFFDILGTIGFIYIIAFSTWAFVRAKDLPRWPIVILLVIG
                     IIGFLVDSTISFKYLSVFQD"
BASE COUNT          937 a          778 c          658 g          909 t
ORIGIN      
        1 ctatctgctc attgccgtgc acaggagagg tttagtgcaa tccctcgttg gtcgtcttcc
       61 tttcggaacg acagggtctg ggaatgaaat gctaaacctc gcctttacac tctaatacaa
      121 gagaactatt tctctcatac tagggtcttc gagaaagtgt caaaatagtc gcacacttga
      181 aaatatgtga tttataatcg gtgtactaac ttcgtgctat agcacgaagt tagcacgcct
      241 agtttttttg agtttgcgag acaaagtcac caaacagggc gcattaaaaa gttccagctt
      301 ttctaaccat gtttgattcc taactttgac tcaatacgtt caactctttt agctaaattt
      361 tcatggatgt ctatttttac tctagtattc gaaaaatcgt caagtcgatt cgccaatcca
      421 tcaatcttct gatttccttt tgctattcca atttctattc ctccaattcg gttatcgagt
      481 attccaaatt gtttctgaac gtcctcaaac ccgcttgcag ttgatagagc aagtttttct
      541 atctggatac ttattttatt cactatgttt tctaattgat ttttatcagc actctttttc
      601 ataaagatat tttatcatgt tttaaatgtt gtcacgaaat gaacccaccg gcctggagtt
      661 cccagagttt ccgatacaag ctaccctccc gttcagtgag gctcttatga gttccctctt
      721 caacaatttt tccatcgtga agcacgatga tccgatccat ttttctgatc gttgaaagcc
      781 tgtgcgcaat aacaattgtt gtttttcctt tcataaccgt atcaaaacca tcttgaatat
      841 acgcttcgga atgagagtcg agacttgacg tggcctcatc gagaataagt atcggcggat
      901 ttttcaatat ggctcgcgca atagcgacac gttggcgttc accacctgat agcttgatac
      961 cacgctcccc tacatacgtc tcatagcgaa gcggtaagct gtcgataaac tcatggcagt
     1021 gcgctttctt ggccgcatca aggacttcct catctgtagc gtccggtctg ccatagcgaa
     1081 tattttccat aagcgtccgg tggaataaga ttgggtcttg cggcacaagc gcaatatttg
     1141 cccgaagact ttcctgcgtc acttttgtaa tatcttgtcc gtcaactaaa atcacgcctc
     1201 cctccggatc ataaaaacgt aacacgagcc gtacgagtgt agattttccc gctcccgacg
     1261 gaccaacaag cgcaattttc tcccccggat gtatcgcgag atcaatgccg ttaagcacct
     1321 gacgcatttt attgaatgaa aacgaaaggc tcttgaattc gatctttcct tctcgaacgg
     1381 agagttcccg tgctccttcg gtatcagcaa tctggtatgg taactccata atctcgacca
     1441 tttcctttgc gtcagcataa ccctgatacg cgtcacgaat gatgcgggtg aagtcccaaa
     1501 gcctcccaaa cattccgatt acgtatgtct gaaaaagtac gaatgttcca accgagatga
     1561 ggccagagcg ccaatatttg atcgagaaat aaaatacaaa aaattcaatg gcaaacatga
     1621 gaaacgactg cactgcttcc atggccgcat caagattcca cgtaaaacgc gacgcttttg
     1681 cctgtgtcaa agtctgatcc ctgaacctaa aaaattctct cactttgccg gtgaagagct
     1741 gaatggtcgt gtgattggtg agatcatctg ccaaaagtcc ggatgtttga gaatcgattt
     1801 cagccacacg gatatcgtat ttgagcttcc aggaagaaaa taaataattg aaacagacga
     1861 agacaacgag ccagacgaga accaaaatcg cgattggcgg catctcgtac cacataacaa
     1921 taataattcc cgtaacccga actagaagcg gcaaaacatt ccaaatgatg cggtcactaa
     1981 ttcgctcaaa agcccgggcg taccgactca gacgttgcgt caacgaaccg acgaaattat
     2041 tgctgaaaaa actatacgaa tgtccgatca tataatcgaa ggcccgctga cggatattag
     2101 cgagcgcgct tgacgtaaaa ttattgttcg acagtgttgc tattcggtat gagagccaga
     2161 ctacaccgtg cataacggct atcatcacta ttatgtgaag caatttatcg ccaatttcgc
     2221 ttgttgttcc cggcatttca aaaacatcga agaatttttt ataaaacgtc ggaacaatga
     2281 tttcaagaat atttgcagaa atgattccga ataacgtaag aaagagcgag acactattga
     2341 gtttcgccga ttgccaataa tgccaaaaaa cgtcgcttag accgactttt ggatatttaa
     2401 taccactttc tgctttagaa cgcatagaac taagagtacc cgattttcac aaatttagaa
     2461 atcagaagcg gatgcgttat cgatattcac atttaaaaag agtgccccgc gcggttggta
     2521 gtctgaacgc aactgaccgc ttcagggaac aataacgaac tggtcagcct acgaaagacg
     2581 ccagtcgctt atattattat tacatattaa taaacatgcg aaaataatca gtcttgaaaa
     2641 acggagagat atttgaaaga tatggtgcta tcaactaaaa aaccaatgat accgatcacg
     2701 agcaaaataa ctatgggcca tcgcggtaaa tctttcgcgc gaacaaatgc ccatgtcgaa
     2761 aaggcaatta tgtagataaa cccaatcgtt cccaaaatat cgaaaaattc agcagacggc
     2821 ataatttttt tgtggcgccg cagattgtct gcggcatcct acaaaattat agtcgtcttc
     2881 gactccttaa ttttgtagac ctagcgagaa tcggctacca cgtagccagg tggttttgtg
     2941 tctcgccgac tcgacataca aaaccctttc gactctcggc gcaaagcaag cagttctcca
     3001 tcgctgtggt cacaaatatt tctcctgacg gagaaatttc gcgaccccgg ctcgtggttt
     3061 ttgcctttcg ctccgctcaa acaaaaacat cactgcgcct tccacaacgt caaaaaatct
     3121 gccctgcggc agatttgttg tacgttgtgg tgttattcta gcatttgcgc gaaccttttt
     3181 tgaacgaaag gactaatcgc acgcgccgcg tgcgtggtgg cttgggactg catcatacgc
     3241 tccgattgcg ctggaatgaa tcccgccacg cgcggagtac cg
//
LOCUS       DATDSU010000107         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1620639, whole genome shotgun sequence.
ACCESSION   DATDSU010000107 DATDSU010000000
VERSION     DATDSU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620639"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VI981_03680"
     CDS             <1..673
                     /locus_tag="VI981_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479426.1"
                     /translation="VTIIEKDERAERPVRQEPVTTDSGNFSVTYRGIFQSEYSYGLVI
                     EDKEGRKTQAQFYTIDTVSNSLTQKDIFLPPTVGLYRTAVTKGDFIKIIGYASPGNTV
                     LIQLEKDISYKVNVDDAGKYQVLINTARLSFGKHSFRARQQDAGTKKESEWSPTKLFS
                     VVAVTVPEADFTGDGVVDVKDWSVFLFRWQAKSGSSGIFDLNRDGKMDISDLSVFLQT
                     FRTLR"
     gene            684..2396
                     /locus_tag="VI981_03685"
     CDS             684..2396
                     /locus_tag="VI981_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEY4479427.1"
                     /translation="MKNLFLTLSTILFSFFPFGAADAATLYFVPESGEFRAGQDIVVE
                     FKADSDGQAFNAAQASVQFSKDTLQVKAIDYSPTSSIFNFWLENPAFSNTDGNISFIG
                     GTTNGISGKSIPLLKIIFVAKGAGEAALTLSDTAITASDGSGTNILETMRPAIFKIVP
                     ETVTPQPVLPVPVRIERTPVAATRLPTAPVLSVPLYSDSSQWHNLISDFLVQWTLPAD
                     ISGISTALNDNPQFVPQAASEGLFDSKMFRGLKNGVQYLHVRFRNTVGWGPTAHYRLA
                     IDTVPPLPFKVESKDGFKTDNPSPTLSFATNDSISGIMHYIAHVDTGDEVTSLKGEFI
                     TPLLPPGLHRIVIRAFDRAGNFTEDAVDMDIIPLPAPVVGFVSRLVLFGEPVFASGKT
                     EPGLGIFARLTDGKSQEVFSGKGTSDTVGNWELIIKNPLSGGRYALTVTAVDERGAQS
                     YPVAAEMVTIRTKPVLSLGPIDLGWFEILLIVIVLTVSGMAIAGQYYISEKKTREAYR
                     IIVGRDIDKFYNLLSDSVKDLEGFKEPRDSVSFVRASASVRKIQETIEKLRKYLRQEI
                     DDIR"
     gene            2754..>3050
                     /locus_tag="VI981_03690"
     CDS             2754..>3050
                     /locus_tag="VI981_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479428.1"
                     /translation="MSSNYWTEEDRWGLEMAKNYRDLNKIALRVLKRMPEPRGELCGP
                     ITSGGAGSVKANLKRFDRAIKKLIADGTEIFSQMPFEAIIQDMRERMNITEYDTY"
BASE COUNT          835 a          622 c          778 g          815 t
ORIGIN      
        1 agttacgata attgaaaaag acgaacgagc cgaaaggccg gttcggcaag agccagtgac
       61 gactgactcg gggaattttt ctgtgaccta tcggggcatt tttcaaagtg aatattcata
      121 cggattagtt atagaggaca aagaggggcg taagacacag gcacaatttt ataccataga
      181 cacggtttca aattctctta cccaaaaaga tatttttttg ccgccgacgg ttggcctcta
      241 ccgcacggcc gtcacgaagg gggattttat aaaaataatc ggctatgcat cgccgggaaa
      301 cacagttttg attcagcttg aaaaagatat tagttataaa gtaaatgttg atgatgcggg
      361 gaaatatcaa gttttgatta acacggctcg tctttctttt ggcaaacact catttcgtgc
      421 caggcagcag gatgctggta ctaagaagga aagtgaatgg tcgcccacga agctattttc
      481 agttgttgct gtcacggttc ctgaagctga ttttaccggg gatggtgttg ttgacgtgaa
      541 agattggagc gtttttcttt ttcgttggca ggcaaaatcc gggtcgtctg gtatttttga
      601 cttaaatcgg gacggcaaaa tggacatttc tgacttgagc gtatttttac aaacatttcg
      661 gacgttaaga taatatacat actatgaaaa atttatttct aacattaagc acaattctgt
      721 tttcgttttt tccgtttggg gcggcagatg cggcaacact ctattttgtt cccgaaagtg
      781 gagagttccg cgctggccag gacattgttg ttgaattcaa ggcggatagc gacgggcagg
      841 cgtttaatgc ggcgcaggca agcgttcaat tttcaaaaga cacacttcaa gttaaggcaa
      901 ttgattattc tcccacgtct tcaattttta acttctggct agagaatcca gcgttctcaa
      961 acacggatgg aaacatatcg tttattggag gaacgacgaa tggtatttct ggcaagtcaa
     1021 ttcctttgtt gaagataata tttgtcgcga agggcgccgg cgaagcggca cttactctaa
     1081 gtgatactgc aattaccgcg tcagatggga gcggcacaaa tattttagaa acaatgagac
     1141 ctgccatatt caagatagtg ccggagaccg tgacgccaca gccagtgcta ccagtgccag
     1201 tgcggataga gaggacgcca gtagccgcga cacgtctccc cacagcacca gtgctttcgg
     1261 taccgctcta ttccgattca tcacaatggc acaatttgat ttccgacttt ttagtacagt
     1321 ggacattgcc ggcggatata tcgggtatca gcaccgcttt gaacgataat ccgcagtttg
     1381 ttcctcaagc ggcatccgaa ggattatttg acagtaaaat gtttagaggt ctcaagaacg
     1441 gcgtgcaata ccttcacgtg cgtttccgga acacggtagg gtggggacca actgcgcatt
     1501 atcgtcttgc catagacact gttccaccgc ttccttttaa agttgaatcc aaagatgggt
     1561 tcaagacaga taatccttct ccaacgcttt cttttgcgac gaacgatagt atttcgggaa
     1621 ttatgcatta tattgcgcac gttgatacgg gcgacgaggt tacttctctc aagggggaat
     1681 tcattacgcc gcttcttcct ccggggctgc atcggatagt gattcgcgca tttgaccggg
     1741 caggtaattt tacggaagat gccgttgaca tggacattat acccttgcct gcccccgttg
     1801 tcggttttgt ctccagattg gtattatttg gtgaaccagt gtttgcgtcc ggcaagacag
     1861 aaccagggct tggaattttt gctcggctta ctgacggaaa gagccaagag gtattttcag
     1921 ggaaaggaac atctgatacg gttggtaact gggaactcat cattaaaaat ccactttcgg
     1981 gcgggcggta cgcgcttacc gtaactgcag tggacgagag gggtgcacag agctatcctg
     2041 tcgccgcaga gatggtaact attcgcacaa agccggtgct ttctttgggc cctattgatt
     2101 tggggtggtt tgagattctt cttattgtca tagtgctgac agtctcgggg atggcgattg
     2161 ccgggcagta ttatatttca gaaaaaaaga cgcgcgaagc atatcgtatc atagtaggtc
     2221 gagatattga taagttttac aacttgttat ctgactctgt gaaagacctc gagggtttca
     2281 aagagccgcg tgattctgtt tcctttgttc gcgccagtgc atctgttaga aagatacagg
     2341 aaacaatcga aaaactaaga aaatatcttc ggcaggaaat agatgacata cgctaacgta
     2401 gagcttaaat tttaggatag agaaatagag gagaaaaggc aaaagagaag tcgggacggc
     2461 atcttcaggg tcagaaaaac aatatctatt attgtactat ttttattacg acgagcgagg
     2521 agtaaaagga aaacttgttt tcattttgtc cgagcgacat cgtgtcaaaa ggtttaatac
     2581 tccacccctt tggggcggga gagagtgagc cccgtcgctc gcgagcgacg gggcgagcaa
     2641 cgcgaacaca aaatgggtgc tccgacttta gtcagagtgg ttagtaccct ttatcttgag
     2701 gttagaacga gtgccatact tcacttttat atttaaggtg ataggataac gggatgagtt
     2761 caaactattg gacagaagaa gatcggtggg gtttagagat ggcgaaaaat tatcgcgacc
     2821 tcaataaaat tgcgcttcgg gttctgaagc ggatgccaga accgcgaggt gaactgtgcg
     2881 gaccaatcac gagcggcggg gccggatccg tgaaagcaaa cctgaaacgt tttgatcgcg
     2941 ccataaaaaa acttattgcc gacgggacag agatctttag ccagatgcca tttgaggcaa
     3001 taattcagga catgcgcgag cgcatgaata tcacagaata cgacacgtac
//
LOCUS       DATDSU010000108         2262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_269786, whole genome shotgun sequence.
ACCESSION   DATDSU010000108 DATDSU010000000
VERSION     DATDSU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269786"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1060)
                     /locus_tag="VI981_03695"
     CDS             complement(215..1060)
                     /locus_tag="VI981_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4479429.1"
                     /translation="MNRKTLLGLFGVLGIWLTAGLALPFVNVLNMFTPPQLMVFRGFL
                     TAAMALIGLRGVIGRVDKYTYLIALVLPFATLGLFEGIRHWGAGPTIIIITATPLVNL
                     VIGTFLGRRVSRASIIGLVLVLGGVVMARWGGHFQWEGFAWTLFGTIMNGILYELFAR
                     AKATSLQKCFYACMGMGTLGLVLSTGASWSAIAEPKLILLVLGFAFVGGFLYWIANLM
                     AFENLPTTEASVLAQGETPAVIFGASVMLGEHLTLVQWVGVVIALYGAWYLSRWLSKQ
                     AVKEQ"
     gene            complement(1181..1837)
                     /locus_tag="VI981_03700"
     CDS             complement(1181..1837)
                     /locus_tag="VI981_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479430.1"
                     /translation="MEVLAKILNWLSENYSWFISLLIQSFIAYHIFFLSKRLSDKAKL
                     EHKERIKKKAEELKLGKEIYLVNVKRYFKDYPSNKEKMFSGYSHIKAEIKTTRFDGIE
                     FICGIKEIYRKPDGSLTLNGKSKKTAREKIKVFEVGVMPYEWIEYIDLRGDEHGFVPL
                     FFGYFKGKRYWQKSWKRFLPFGYPYKEIIYYKESGVYHEGSDPVDMKFSFIDEPISKK
                     "
     gene            complement(1842..2048)
                     /locus_tag="VI981_03705"
     CDS             complement(1842..2048)
                     /locus_tag="VI981_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479431.1"
                     /translation="MSDSSKKLALNMKKIRTRKHMSQGDICRELGVDRAYISTIESGK
                     QNPTLSTIERIAKALGVSVDELLK"
     gene            2082..>2262
                     /locus_tag="VI981_03710"
     CDS             2082..>2262
                     /locus_tag="VI981_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479432.1"
                     /translation="MTQWCDKIQYRYADGEAKPKISFPFLFPLRGGGVGGGGARKMQG
                     NFLVFAHASPRACPRR"
BASE COUNT          616 a          530 c          482 g          634 t
ORIGIN      
        1 ccgactgagt tcggtggtaa ctcataaagt ggattgtgct aggcacaaac aaatctatat
       61 gtgcttgccc cgcccgccct gtgcgcgcac aaatccccgc cgtttttagg agacattata
      121 aaacggcggg gattgctccc ttggattaaa atttatagta aagaaaaagg tctcgaagcc
      181 gaagctccga gacccatgat gtggcttacg ccatttactg ttccttaacc gcttgcttcg
      241 agagccagcg cgagagatac cacgctccgt agagcgcaat cactacgcca acccactgaa
      301 caagcgtgag atgttcaccg agcatgacgc ttgcaccaaa gatgaccgca ggggtttctc
      361 cttgggcaag gacgcttgct tccgtcgtgg gcagattctc gaacgccatc agatttgcaa
      421 tccagtagag gaatcctcca acgaacgcaa atccgagaac gagcagaatg agttttggtt
      481 cagcgatcgc tgaccacgat gcaccagtgc taaggacgag gccgagcgtt cccataccca
      541 tgcaggcata gaaacacttt tggagcgatg ttgctttcgc tctagcaaag agttcgtaga
      601 gaatgccgtt catgatagta ccgaacagcg tccacgcgaa cccctcccac tggaaatgtc
      661 cgccccatct tgccatcacc acgccaccga gcaccagaac gagtccaatg atcgaagctc
      721 gcgaaacccg tcggcctaag aaagtaccga tgacgagatt caccaacgga gtcgcggtaa
      781 tgatgatgat tgtcggacca gcgccccaat gacgaatgcc ctcgaacaat ccgagtgtgg
      841 cgaacggcaa gaccaaggcg atgaggtagg tgtacttgtc cacgcgtccg atgactccgc
      901 gaagtccgat gagtgccatt gcggcagtca agaacccgcg aaagaccatc aactgcggcg
      961 gcgtgaacat gttgagaacg ttgacgaaag gcagggccaa ccctgccgtt agccaaatgc
     1021 ctagaactcc gaagagtccc agcagggttt tgcgattcat ttctggaccc tcctttcagt
     1081 tttttggttt ggaaatgtgc tgtctttgag ccagaatagc tcaaaaatcc ttttgctatc
     1141 ctagcataag gatagcattc tgtcaagaca aaaggtattt ttatttcttt gaaatcggct
     1201 cgtctataaa actaaatttc atatcgactg ggtcgctacc ctcatggtaa actccacttt
     1261 ctttgtaata aataatttct ttataaggat agccgaaagg taaaaaacgt ttccaagatt
     1321 tttgccaata tcttttacct ttaaagtagc cgaagaatag cggcacgaag ccgtgctcgt
     1381 ctccccgcaa atcaatgtac tctatccatt cgtaaggcat tacaccaacc tcaaatactt
     1441 taattttctc tcgtgctgtt tttttgcttt taccattcag cgttaaactg ccatcaggtt
     1501 ttctgtaaat ttctttgatg ccacaaataa attcaatgcc atcaaatctt gtagttttta
     1561 tctctgcttt tatgtgagaa tatccactaa acattttttc tttgtttgat ggataatctt
     1621 taaaatatct ctttacattt acaagataaa tttctttgcc cagttttaat tcttcagctt
     1681 ttttctttat tctttcttta tgttcaagtt tcgctttatc tgataatcgt ttagatagaa
     1741 aaaagatgtg ataagcaata aaactttgta tcaaaagact tatgaaccag ctgtagtttt
     1801 ctgataacca atttaagatt ttagctaaaa cttccataaa attatttcaa tagttcgtca
     1861 accgaaacac caagagcttt ggctattctc tcaatagttg aaagtgtcgg attttgtttc
     1921 cccgactcta tggtgctgat ataggcacga tcaacgccaa gctctcggca tatgtcgcct
     1981 tgcgacatat gctttcgtgt cctgattttc ttcatattca aagctagttt tttcgatgaa
     2041 tctgacataa ttgttatctt gagtatagac aatgttgtgc cttgacacaa tggtgtgata
     2101 aaatacaata tagatatgcc gacggcgaag caaaaccaaa aatttccttt ccatttcttt
     2161 ttccgcttcg cggcggaggg gtggggggag gaggcgcgag aaaaatgcaa ggaaattttt
     2221 tggtttttgc acacgcgagt ccgcgagcgt gtccgaggcg cg
//
LOCUS       DATDSU010000109         5518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_271164, whole genome shotgun sequence.
ACCESSION   DATDSU010000109 DATDSU010000000
VERSION     DATDSU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271164"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VI981_03715"
     CDS             <1..260
                     /locus_tag="VI981_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479433.1"
                     /translation="VGEEQYLRAQGAVELIFVVVLLAWFIPRKYVFAVASLIFVEMLS
                     ILILVGIKGDTFRDIGLVGAALALMVLLKKKSSPQTIHESR"
     gene            552..2837
                     /locus_tag="VI981_03720"
     CDS             552..2837
                     /locus_tag="VI981_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY4479434.1"
                     /translation="MSDLPPAKLGGRIRSYLVISVFALFAVILFGLLWLFFLAPSQQS
                     GFGWYVFSFAAGLSMIVLPCTLPLAFVIVPLSMGKGVAKGLGIALSFGSGVALMLSIY
                     GVIAAAVGGAAINGLGADLETVKNWVYFIAGIFALIFALGEIGLLKFRMPSYTGAAPA
                     FIQKKQDFIKAFLLGMFLGNIGVGCPHPATPLILVEIASSGQVFYGWTLFLIHAIGRI
                     LPLLFLALLGILGVNGLSWLVARKEKVEKATGWAMVFVAGFILTLGLFTHKWWVNSGI
                     HTLLERVTGEEQFLEVVRTNLNSTQTHRHGLEMGTGLFGLPLWLGNWFLVLVWVVPLW
                     WWWFRERKRVSGIPPDAKIDEKNAEHKILGWKEKFITILMILFGAIFIYILPQNFLRQ
                     QSGDMHALSESADHEHTPEEMMDPDHIDEMANDSSHMNEMGESDGHGHSAQYHEETFV
                     KEGIVVNLGVPANISAGETANLAFYVNTKPASTPVVNLELGHKKYLHVIGVRSDLSQF
                     FHIHPENSGSGFWSSNYAFKEEGKYKLFTDIKYAGQQHTFGHGAIAVRRTLTSPKTPA
                     PQVQFFDNVIVGNYQVALDHNVPLVKGKPMQFIFTYQDVLGNGVELEPYLGADMHLVV
                     IKDDLSEYVHAHPDDSTTSSDDGHDHEHTFLGAQQAYAHTGATTGGPDPAEIPFTVTF
                     PKEGIYKLFGQFRPKGTDLPVETALLAEFYVKVESSGVSVPPEYHDHESSGVPSRALM
                     VVVSLILMTALSYGVAKFLKK"
     gene            2941..3378
                     /locus_tag="VI981_03725"
     CDS             2941..3378
                     /locus_tag="VI981_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY4479435.1"
                     /translation="MNKYVIILIVLAVIVGASIVFKDSLGTNDNKVVATGIVREITIR
                     AKQNEWRFDPDVIEVDQGDTVKLTVINEDDFDHGIQIDAFGVSKRIPANGTVKAEFVV
                     TKSGEFPFFCSVPCGDGDVADGTHRGHFDQKGKIVVRNAIKTQ"
     gene            3409..3729
                     /locus_tag="VI981_03730"
     CDS             3409..3729
                     /locus_tag="VI981_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479436.1"
                     /translation="MNLPIKKLFWLKSALVGFLISIIFFWVKLMLTTSDVAPPICNYD
                     GDWEIVGMPCSATEFIVQNYIEEIITVTIVFAILGWLYSKIFTRGGSAVGGKNRRNSV
                     LPKP"
     gene            3915..>5518
                     /locus_tag="VI981_03735"
     CDS             3915..>5518
                     /locus_tag="VI981_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY4479437.1"
                     /translation="MTKLVLKVKGMHCASCSVLIDKLLIKQLGVFSVKTSYGAEKTAI
                     EFDEGKITLNKIDEFINKLGYDLIRPEEESATLEEEETKEAKLIKQAKKRVIAAFVLA
                     APIIVYYMLIHMFNLKHVHEIAGLDLNYVYWILSTPIQFIIGWPFYRNAFTSIRVGAA
                     NMDVLVVLGTSAAYLYSMIGFLFFELDHPFWESSAALLSFILLGRYIEVLAKGRASNS
                     IKELLKLEAKDAHVVRDGHEVTIPINQIALGDIVVVRPGEKVPVDGIIVEGKSHIDEK
                     VVTGESFPVGKKVDDEVIGATINQEGLFKFKVTKVGKDTLLNQIIKMVEEAQASRAPI
                     QDLVDKISEYFVPAVIVLSVLVFCGWYFFVLGGDIGVNATNATAFRSALMKMIAVLVI
                     SCPCAMGLATPTALVVGIGRAAKYGIILKGGEALESAYRVTAIIFDKTGTLTIGKPQV
                     TDVVMLTSDYNDQNAVKFAASLEQGSEHPLARAVVEKGKNMFHELDPVSEFEAIAGKG
                     LKGKVADKRVDIGNLTYMESLAVDVK"
BASE COUNT         1515 a         1122 c         1297 g         1584 t
ORIGIN      
        1 cagttggtga agagcaatat ctgcgcgcgc agggagcagt cgagcttatt tttgttgttg
       61 tattgctcgc atggttcatt cctcgaaaat atgtgttcgc ggtagccagc cttatttttg
      121 tcgagatgct gtcaatcctt attttggttg gaataaaggg cgacacattc cgcgatatcg
      181 gtcttgttgg agcggcactt gcgcttatgg tcttgcttaa gaaaaagtca tctccacaaa
      241 cgatccatga gtcccgttag agattaaata gttatgtata atcatcggcc gaaaaataaa
      301 aaattcactg gaaagaaacg attttggcta ggaattttac ggaaatgcaa tattgattta
      361 tccgttgccg caccgaattg gattataatc gaagataatg aatgttctgc aaacaggcag
      421 atgcatttcc tacctgcccg ccacagccag agcgaaatac gcagtagctt taatataatg
      481 atgatattac caattaatta ctctacctcc cattatgtgt gtcaggcgat ggcaggcggg
      541 tctctaacgg gatgagcgat ttgccgcctg caaaacttgg aggaagaatc cgttcgtacc
      601 tcgtcatttc tgtttttgca ctttttgccg taatcctatt tggactactt tggctatttt
      661 ttctagcgcc atcacaacag tctggatttg gctggtatgt ctttagtttc gcggctgggc
      721 tttcgatgat cgtgctgccg tgtacattac cgcttgcatt cgtgatcgtt cctctttcta
      781 tgggaaaagg tgtggccaaa ggactcggga ttgcgcttag tttcggatcg ggagtagctt
      841 taatgctttc gatttacgga gtcattgccg ccgctgtcgg aggagcagct atcaatggac
      901 ttggagctga tcttgaaacg gtcaaaaatt gggtttattt catagccgga atttttgcgc
      961 tgatttttgc actcggtgaa atcggacttc tgaaattcag aatgccgagc tatacaggcg
     1021 cggctcctgc attcatacaa aaaaaacaag attttattaa agccttttta ctcggaatgt
     1081 ttctcggcaa cattggcgta ggctgtccgc accctgcgac acccctcata cttgtcgaga
     1141 tagcatcatc tggccaggtt ttttacggtt ggacactttt cctgattcac gcgatcgggc
     1201 gcattttgcc gctattattt ctagcgctac tcgggattct gggagtcaat ggtctgtcgt
     1261 ggctcgtggc gcgaaaagaa aaagtagaga aagccacagg gtgggcgatg gtatttgtgg
     1321 ccggattcat tttgactctc ggactcttta ctcacaaatg gtgggtaaat tcaggcattc
     1381 atacactact agaaagagtg accggcgaag aacagtttct cgaagttgtc cggacaaatt
     1441 taaacagtac gcagacacat cgtcatggtc ttgaaatggg aactggcctt tttggcttgc
     1501 cgctctggct cggaaactgg tttttagtgc ttgtatgggt tgtaccgctt tggtggtggt
     1561 ggtttagaga acgcaagcgc gtttcgggaa ttcctcccga tgcaaaaata gacgagaaaa
     1621 atgccgagca caagattctc ggctggaaag aaaaattcat aacgatactt atgattttgt
     1681 tcggagctat ttttatttac attcttcctc aaaatttctt gcggcaacag tccggcgata
     1741 tgcatgctct ttctgaatcg gcggatcacg aacacacgcc ggaagaaatg atggacccgg
     1801 atcatataga cgaaatggcg aatgattcta gccacatgaa tgagatgggc gaatctgacg
     1861 gacacggtca cagcgcccag tatcatgaag agacttttgt taaagaaggt atcgtagtca
     1921 atctcggcgt cccagccaat atctccgcgg gggagactgc taaccttgcg ttttacgtga
     1981 atacgaaacc agcgagtact ccggtcgtga atcttgaact tggacataaa aaatatctcc
     2041 acgtcatcgg tgtacgcagc gacctaagcc aatttttcca cattcatccg gaaaattctg
     2101 gatcgggttt ctggagctca aactatgcat tcaaggaaga aggaaaatat aaattattta
     2161 cggatataaa atatgcgggg cagcagcata cgtttggaca cggagcgatc gctgtacgac
     2221 gtaccctaac ttctcccaaa acaccggctc cgcaagtgca atttttcgat aatgttattg
     2281 ttggcaatta tcaagttgcg ctcgaccata atgtgccgct cgttaagggg aagcctatgc
     2341 aatttatttt tacgtatcag gacgttttgg gaaacggagt ggaacttgag ccttatcttg
     2401 gcgcggatat gcatcttgta gttatcaaag atgatctaag tgagtatgta cacgcccatc
     2461 ccgatgattc gactacaagt tctgatgatg gtcacgatca tgagcataca tttttgggag
     2521 cacagcaggc ctatgcacat acgggtgcaa cgaccggagg gccggatccc gctgaaattc
     2581 cgttcacagt aacgtttccg aaagagggaa tttacaaact cttcggccaa ttcaggccca
     2641 aaggaacaga cttaccagtc gagactgcgc ttctcgctga attttacgta aaagttgaaa
     2701 gttcgggtgt ttcggttccg ccagaatatc atgatcatga atcttctggg gttccgtcgc
     2761 gcgcgcttat ggtcgttgtc tcccttatac ttatgacggc tttgtcctat ggagtggcta
     2821 agtttctaaa gaagtaaaat accaatatac gaatgaatgc taatatgcaa attaatacta
     2881 atccctcatt cgtattcatt agtatattcg taataattag taaattagta ttgtgtcagc
     2941 atgaataaat acgtcataat tctaattgtt ctcgcggtaa tcgtcggggc cagtattgtt
     3001 tttaaagata gcctgggtac aaatgataac aaagttgtcg ccacgggaat tgttcgagaa
     3061 attacaataa gagccaagca gaatgaatgg cgttttgacc ccgacgtaat tgaagtggac
     3121 cagggcgata ctgtaaaact gacagtaatc aatgaggatg attttgatca cggtattcag
     3181 atcgatgctt ttggagtgag taagcgcatt cccgcaaatg gtacggtgaa agctgaattt
     3241 gtcgtgacga aatccggcga atttccattc ttttgttcag tgccgtgcgg tgacggagat
     3301 gttgcagacg gtacacatcg cgggcatttt gatcagaaag gaaagatagt ggttcgaaat
     3361 gcgattaaga cacaataata attttcaagt atcaatttac aattttcaat gaacttaccg
     3421 attaaaaaac tattttggct aaagagtgct ctggtaggct ttttgatcag cattattttt
     3481 ttctgggtaa aattgatgct tactactagc gatgtagcac caccgatctg taattatgat
     3541 ggagactggg aaatagtagg tatgccatgt tctgcgacgg aatttattgt tcaaaattac
     3601 attgaagaaa ttattacggt cactatcgta tttgctattc tcggctggct ctacagtaaa
     3661 attttcaccc gaggcggatc cgccgtaggc ggaaaaaatc gtcgaaattc tgttttacct
     3721 aaaccctaaa cgctactccc taaacgcttc ttaaaaatat gaccaaactt gttttaaaag
     3781 taaaaggaat gcaatatgga aaattcgaaa ttcgaaattc gaaattcgaa gcaagaaagt
     3841 tttttgaatt taattatttt aatttagata ttgttttgga tttcggattt agtgcttcgg
     3901 atttataaaa atatatgact aaattggtgt taaaggttaa ggggatgcat tgtgcttcat
     3961 gtagcgtact tatcgacaaa ctgcttatta aacagctagg cgtcttttct gtgaaaacaa
     4021 gttatggtgc ggagaagact gcaattgaat ttgacgaggg gaaaattacg ctcaataaaa
     4081 tagacgaatt cataaataaa cttggctacg atttaatacg acctgaagag gaaagtgcaa
     4141 cacttgaaga agaggagacg aaggaagcga aactaatcaa gcaagctaaa aaacgggtta
     4201 tcgcagcctt cgtgctcgcc gcgccgatta tcgtctatta catgctcatt cacatgttta
     4261 atcttaaaca tgttcatgag atagcgggat tagatctcaa ttacgtatac tggattctct
     4321 ccactcctat tcaatttatt atcggctggc cattttacag gaacgcattt acgtcaattc
     4381 gagtaggggc cgcaaatatg gacgtactcg tagtacttgg cacgtccgct gcttatctct
     4441 acagcatgat cggttttctc ttctttgaac tcgaccatcc gttttgggaa agttcggcgg
     4501 cccttctctc gtttattttg ctcggacgtt atatcgaagt acttgccaag ggccgtgctt
     4561 caaattctat taaagaactc cttaagcttg aagccaaaga tgcgcatgtc gtgcgcgacg
     4621 gacatgaagt gacgatcccg ataaatcaaa tcgccctcgg cgacattgtc gttgtccgcc
     4681 cgggtgaaaa agtgcccgtt gacggcatca ttgttgaagg caaatcgcat atagatgaaa
     4741 aagttgtaac cggtgaatct tttcctgttg gtaaaaaagt tgatgacgag gttatcggcg
     4801 cgacgatcaa ccaggaaggt ctcttcaaat ttaaggtaac taaagtcgga aaagacacat
     4861 tgctcaacca gattatcaaa atggtcgaag aagcgcaggc aagtcgtgct ccgattcagg
     4921 atcttgtcga taaaatcagc gaatatttcg ttcccgccgt catcgtgctt tctgtgcttg
     4981 ttttttgcgg ttggtatttc tttgtgctcg gcggtgatat tggagtcaac gctaccaatg
     5041 ctactgcctt ccgttcagcc ttgatgaaga tgatcgctgt actcgttatt tcttgcccgt
     5101 gtgccatggg cctggccacg ccaacggcgc ttgtagtggg tatcggacgc gcggcaaaat
     5161 acggaattat cttgaaaggt ggagaagcgc ttgaaagtgc ctatcgagtc accgctatca
     5221 tttttgataa gacaggaacg ctcactattg gtaaaccgca ggtcacagat gtcgtcatgc
     5281 ttacatccga ctacaacgat caaaatgccg tcaaatttgc cgcctcgctt gagcaagggt
     5341 cggagcatcc gcttgcacgc gcagttgtcg aaaaaggcaa aaatatgttc cacgaacttg
     5401 atcctgtttc tgaattcgag gcgatcgcag gaaaaggact gaaaggaaaa gtagccgaca
     5461 agcgcgtcga tattggtaac ctaacctata tggaatcttt ggctgttgac gtaaagga
//
LOCUS       DATDSU010000110        27090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_940386, whole genome shotgun sequence.
ACCESSION   DATDSU010000110 DATDSU010000000
VERSION     DATDSU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 27090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27090
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940386"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /locus_tag="VI981_03740"
     CDS             <1..2133
                     /locus_tag="VI981_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479438.1"
                     /translation="KEGVKIQDIADERLTFVSETHTGNINAGYKGSAPYNPSSRMVKW
                     HTTFFRAGDAGTVTWRAKIGVTEEECSVVEIPNSARIGAILGDDEDEWVWTYSNTVKT
                     TVGSTCECRLVISKLVNIANAGNGDVLTYTVNFKNEGDADCTGGGVKIEDTLDSHLVY
                     LSEAHASNVTPGYGSRGVYTASDRIVRFNAGTLNPQESGWFSFNVRVADAPCSGYDVP
                     NRARASAEELNWNWIESNTVSTHIESNDCNQSVLYGSCEASPTSGRVGDGFVFSANAT
                     GGNGVFSYSWTGTDGLRGSSRNVNKTYTSAGLKNATVSITSGNTIINKTCTVNIQEPP
                     VVGGNFSAVCSVNQTSGVQGSLFTFNAIPTGGTGYYTYSWTGDDNLIGTSQSVQKGYM
                     TDGRKDATVAVRSGHETITRTCSVDVTHTTTGCSTCGGIGVDQPVAKLTSSKVPTQPE
                     AAFVFLSQIPYTGIDTYGEIFLYFLGVALWSVLAAGYVVYRKTRKLPILKFSRTEAYA
                     SGSMATVPMNLPTEARAPQIRAELTLIEKLEQYARENSALVSLGGLNKVIELSGSDEV
                     KAKEILKYVVESYKANDTYDWVTLDEAKVEQSLAVNESPFSRFVSALEQNDVDRIRMF
                     LKEVEKTGMTLKQFAESVVRELDEVYRHRTGVKNSANMHIVERLKEWRHEHIHAVKTA
                     MENSLNSALHTPHVAVKLGILRAAEIIA"
     gene            complement(2349..3212)
                     /locus_tag="VI981_03745"
     CDS             complement(2349..3212)
                     /locus_tag="VI981_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479439.1"
                     /translation="MRHPEAFIGENEKFVNVPAAYERAEKVLANPIDPRDFADIYKDV
                     DADIKWVGEMEAKFREDERQHPERKHFMMVGRCLEAIVYESDQHNWFGENATILQTSR
                     FDDIKNGTDSIVEIQEGSGASHLALGIDVTTSTRLAENLERILTEIRDNRLAEIKYFQ
                     SDHLSIRGHMSNIPRVVIGADKASVYEVIDTWMSGKNKELAIHPMQHKILEEIRIQLG
                     VFREYAEHIGKTDLARIYENTLLKINEIIESNPKVSDETMLAIRSDEVFDSIAVYLDS
                     IRVGMRNEQAA"
     gene            complement(3209..4957)
                     /gene="thrS"
                     /locus_tag="VI981_03750"
     CDS             complement(3209..4957)
                     /gene="thrS"
                     /locus_tag="VI981_03750"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY4479440.1"
                     /translation="MISAMASVNEIGNIRHSLAHLLAAAVLELWPTAKNTIGPAIENG
                     FYYDFDFGSEKISDADLPKIEKKMREIYKSWREFGYKEVREAEAREIYKDNSYKLELL
                     NDIKSRNEKITLYTSGAFTDLCRGGHVDEMRSIAIDSFKLSHIAGAYWKGDASRPMLT
                     RIYGLAFHKKEELQKYETQLEEAKKRDHKKLGRELGLFTLSPLVGSGLPLMQPKGMII
                     RKAIEDFLWDLHRKRGYSRVWTPHIAKEELYVTSGHAAKFGDELFRVQGKEEKFIMKP
                     MNCPHHMQIFADNQWSYRDLPVRYFEHATVYRDEKSGQLGGLTRVRAITQDDGHLFCR
                     VDQIKEEVSTIVQVIREFYDRFSLNDYWVSLSVRGEDKSKYLGAEDSWKKAEDALDDA
                     AKANNLNYTRVVGEAAFYGPKLDFIFKDSIGREWQLATIQCDFNLPERFDLSFTNDRS
                     GKERPVVIHRAISGSLERFMGILIEHYAGAFPYWLAPIQVAIVPVSEKHHEYANKCEV
                     LLNDGDIRTEMFNSNDTVGKRIRLFKMQKIPYAIVIGDKEQADDTVTLENRDTGKSTV
                     LKQDTLVDFFKNNLLK"
     gene            5025..5402
                     /locus_tag="VI981_03755"
     CDS             5025..5402
                     /locus_tag="VI981_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479441.1"
                     /translation="MTRENTRQNETVFGISFVFGRPLRIGAGKDTYSRRQESRSALAV
                     SEVKSPRVSGPTTRKSEMDRSIRINPVIRQTTKRQRETSRTIILCIAGEYSCTKRQKN
                     KKKREPCGAVRALAGFGWRHYRV"
     gene            complement(5535..8717)
                     /gene="dnaE"
                     /locus_tag="VI981_03760"
     CDS             complement(5535..8717)
                     /gene="dnaE"
                     /locus_tag="VI981_03760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY4479442.1"
                     /translation="MSKFIHLHTHSHYSLLNALPQIPDLVKRAKDNEMSALALTDSGN
                     LYGAIEFYKKCKDFGIKPIIGVDAYVALRSRHDKQAGIDNRRSRLVILARNNEGYKNL
                     IKMVTDSYLEGFYYKPRIDDELMEKYKSGLLAIIPSFSGETSQSLKSSDPSKAAEIVG
                     KYKSIFGAENVYLEISHHPEIEGHADLMKKVERLGEATTTPLVAAHDVYYLDPEDRRA
                     RETLMSVQTNADFIERRDSSGTEENFSFTSGKQMESYFRSMPSAIANSFEISEKCNVE
                     LELGKWVFPNYVVESGLSYNDELRRLVYEGIKKRELQETAEITKRVEYELGVIFNKGY
                     SPYFLVVSDLLRFAHERDILTTIRGSVAGSLVTYLAGITNVNPLEYKLPFERFLNPER
                     PSAPDIDMDYADNRRDEVIEYAKQKYGKDRVAQIGTFGTMMARGSVRDTARALGYPYS
                     LGDRIAKLIPMGAQGFPMTINRALDEVPDLKKIYKAEKEVKDIVDMAKKIEGCARHIS
                     VHAAGVVIAPSALTNYVPLQFDPKGEGKIITQYDMHAVDDAGLLKFDFLGIKNLSILS
                     DSVKVTKKVYNVDVEIEHIPLNDKKTFSLLAKGETIGLFQLNGAGMTAYLKQLRPTTI
                     FDINAMVALYRPGPMESIPLYIERKHNTERVSYLDGRLADILSQTYGVITYQDDVLLI
                     AVNLAGYSWLEADKLRKAMGKKIPSVMEAEKEKLTNGLVKNGMTEANALELWKLIEPF
                     AAYGFNKAHAASYGRVAYQTAYMKANFPAVYMASVLTADSGDVEKIGEIIAECKRMKI
                     PVLAPDINGSFSGFTVVRKDGKEENMIRFGLTTIKNFGEGIANTIIAERKKNGKFGSL
                     ANFLDRIKDRSLNKKSLEALIKCGALDQLGERGIMLANIEDLLAYNKEKTNENDNQNS
                     LFGATAPDANTLTLRQAEPASQGNKLAWEKELLGLYISGHPLDKFKDKIDKHDMPIKK
                     IKEQMKEGMMTVVAGIIEEAKDLLTKKGDKMMFLTLADFTGNIETVIFPRVYEENKSM
                     LQLEKCIAIKGRFSERNGRQSIIAEKIKEL"
     gene            8833..9462
                     /locus_tag="VI981_03765"
     CDS             8833..9462
                     /locus_tag="VI981_03765"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY4479443.1"
                     /translation="MLSETAQRCAIKYLIGIDEVGRGPLAGPFTVGAVAIPFGSKLYL
                     GAVKDSKQLLPEDRDRLFRKINALRKDGKLRHSISHIAPHVLDKIGLTKSAHSAVARS
                     LKKLKVKPDECLVLLDGGLRAPNEFLNQRTVIKGDEKIKIIALASIVAKVTRDRTLIR
                     LSKKYPEYDFHVHKGYGTKRHYERLRLYGPCEIHRKLFLRKFFESCSTV"
     gene            9517..9792
                     /gene="rpmF"
                     /locus_tag="VI981_03770"
     CDS             9517..9792
                     /gene="rpmF"
                     /locus_tag="VI981_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY4479444.1"
                     /translation="MVVRMRHNRSQTGNTRSHHALKPARLSKCQDCGHMHLRHSMCQN
                     CGKYMGVVVVDVHAKMEKKEARRKARLKERGEEVKKAETETADVKKS"
     gene            9869..10798
                     /gene="nusB"
                     /locus_tag="VI981_03775"
     CDS             9869..10798
                     /gene="nusB"
                     /locus_tag="VI981_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY4479445.1"
                     /translation="MANRHLARSTVLQTLFEWDFAGKKDTDAQEILMRNAKEFASGIG
                     DTSFLQHLLGNVLGQREKLDEIITKAAPEWPLEKISVVDRNILRMGLFELLFSDRNEV
                     PAKVAINEAIELAKTFGGENSGRFINGVLGSVYKEIGEPGKDSPARPRKKVVDLKDMP
                     IEKLAGAVVYARREEDIFLALVHDIFGHWTLSKGHLNEAEIEKEGVVRVIKEEINLDL
                     EVREELGRNEYIASDPEKGKIRKQVIYFLAESPFVDIVLAKSGGLDDARWFKLSDILD
                     LNFYNDILPLVTKAVQILLSRSAEETVMSRKTK"
     gene            10943..11686
                     /gene="rnc"
                     /locus_tag="VI981_03780"
     CDS             10943..11686
                     /gene="rnc"
                     /locus_tag="VI981_03780"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY4479446.1"
                     /translation="MPRAVLRLKTIRGNTSKYMDYPDFEKHIGVSFKNKALLLQAFTH
                     RSYLNENKRNSLEHNERLEFLGDAVLELVITVFLFNKYPDKPEGELTSYRAAVVNATT
                     LSNVAKSLLMEPLLRLSHGESKDTGKARQIILANTIEAFIGAVFLDQGYGTVEKFILK
                     HIASLLEKIVEKGAWIDAKSYFQEQAQEQVGITPAYRVVRENGPDHDKKFIIGVYLRD
                     EKVAEGEGKSKQEAEQMAAEAGLKAKKWK"
     gene            11877..12389
                     /locus_tag="VI981_03785"
     CDS             11877..12389
                     /locus_tag="VI981_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479447.1"
                     /translation="MDTSFVMLRLNEVAEQLKPIAGYLGFSRLGEIMDLKTPIGHTQT
                     RLFRLFPRQVYVPLDALCFDSRVRRLIDFAKPDFSAPITTVDQLVIAQDLRWFRWKTV
                     YRLVSETYAEALSSTMQLVVDRSSVPEYTVWLERLDKAIDEWIVESMGRRSEICTKLE
                     MVRRKHFETA"
     gene            12533..13027
                     /locus_tag="VI981_03790"
     CDS             12533..13027
                     /locus_tag="VI981_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479448.1"
                     /translation="MKPFNSIAHELQSCKDALRFNKVSDYLNRQEHDVPIQIVSGHLL
                     LPVGIGLDSPLKILYTVSDDYEGDCKQFCREELGYTRAYRPWIRWRTVYERASLPHTC
                     YFGRESTIHVADMYQVLAIFSNPDRRSELEASVVEWIAESVKRRLALIEALKESSDRL
                     TTGT"
     gene            13138..15423
                     /locus_tag="VI981_03795"
     CDS             13138..15423
                     /locus_tag="VI981_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4479449.1"
                     /translation="MLKSLELSGFKSFAKKTDLDFKTAITAIVGPNGSGKSNVAEAFR
                     FVLGEQSIKSMRGKKGEDLIFNGSNKIPKMNRGGAKVIFDNRKRILNIDFDEVSIERV
                     VHRDGINEYSINGSQVRLKDIIELLAGAHIGATGHHIISQGEADKILNANVRDRREMI
                     EDALGLKIYQYKRQESARKLEKTAENIAQVESLRREIAPHLKFLRKQIEKLARAEVMK
                     QELVLLYCQYFKRENIYLTSEKNFIETEKRKPVAELRALEEELTHARKQLEGMSDAKM
                     ATQLFELEGKIAGARHAKDTLTRDFGRVEGEIAGVERARKREEDAAAEHDLHTLSFKE
                     FESLVESIFESIHEAEKTDALDKIKSLLAHVRESIKKFIEKNRASSHRKNGEIFKTEL
                     ERLGGEKTALERDLSRAVDTERSHVKEYESIKSNIEMERRGGLEAEKNILRIMTRQNE
                     INSAYASVKAREERHHIELADFNRDLTEAGHIAGHDAINFEDTVIVRQDKTSIGEREI
                     AEEPRDSQRERRRVIERIKIRLEEAGLAGSADLIKEFKESEERDQFLGKEIEDLTKSS
                     VALQALIDELQQKIDSEFKEGIKKINAQFQDFFALMFGGGSASLDVVEEIRKRASAIG
                     EIDADGIDELSVTDETEVPEIGVEIKVHLPHKKIRGLEMLSGGERALTSIALIFAMSQ
                     VNPPPFLVLDETDAALDEANSRKYGDMIENLAKYSQLILITHNRETMSRAGILYGVTM
                     GGDGTSKLLSIAFEEAVNVSK"
     gene            complement(15778..17745)
                     /gene="rnr"
                     /locus_tag="VI981_03800"
     CDS             complement(15778..17745)
                     /gene="rnr"
                     /locus_tag="VI981_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY4479450.1"
                     /translation="MDKTHKRDRAKEKNTEVSLQGTINVTSKGVGYFEHDDYAEDIEI
                     QPELLNKALNNDEVLVKVLRTKKNKRIQGEVVRVTKRAKTEFVGTLECEQGSYFLVPD
                     DRRFYKDISLGKLQNMESGVKALVSLSRWDKKGPEGKLIKVLGKKGLHDVEMQSIIYD
                     KGFETTFHADVEREAEHIAKTLLPIPQVEINTRRNFRDVTTFTIDPVDAKDFDDAISI
                     RSVGTNIYEIGVHIADVSHYIRPHTKLDKEAQKRAFSVYLVDRTIPMLPEALSNGICS
                     LKPDEDRLTFSVVFKIDMSGTILDRWFGKTIIHSAKRFSYEEAQKVLDARSGGHFESL
                     HILNSIAHRLRVHKFAKGAIDFEKEEVKFELDSNGKPIRVYKKDRLDTHMLVEDYMLL
                     ANREVAEYIVKENKKRKIDGGAFIYRIHDTPNQDRLADLELFIKALGYDLESHDGKLT
                     AKSIKKLLNDVTGKPEESLVKTATLRSMAKAIYATSNIGHFGLAFEHYTHFTSPIRRY
                     PDLLVHRVLFEYLNGREIPKHELAFYYKMAIQSSEREVLAAEAERASIKYKQVEYMKE
                     HIGEEFNGIISGVTEWGIYVEEVETKCEGMIKLRDLGDDFYALDQKNYRVVGEKAKKT
                     YSLGDKIRFRVAGADMERRTLDFQLAERGFM"
     gene            17949..18347
                     /gene="rpsP"
                     /locus_tag="VI981_03805"
     CDS             17949..18347
                     /gene="rpsP"
                     /locus_tag="VI981_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY4479451.1"
                     /translation="MLKIRLQRVGRKKQPYFRFVLTDSKNSTKSGKFHEVLGFFDYRK
                     GGEHKIDKARVVFWMSKGAQLTPSVHNYLVGEKIIDGKKINVLPRKSPPKPEEAKKED
                     KKTEKAAKVKNEETITEAPKQENTVESTAS"
     gene            18482..18790
                     /locus_tag="VI981_03810"
     CDS             18482..18790
                     /locus_tag="VI981_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY4479452.1"
                     /translation="MEQDAQFLDYVIKALVDNPNDVKITRTVDEMGVLLTLTVNPADM
                     GKIIGRMGNTAKAIRTLLRVVGMKNNARVNLKINEPEGGTKPERASKTVDEAMEDLKI
                     "
     gene            19668..20432
                     /gene="trmD"
                     /locus_tag="VI981_03815"
     CDS             19668..20432
                     /gene="trmD"
                     /locus_tag="VI981_03815"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY4479453.1"
                     /translation="MTFHIITLFPSMFDSYLKESILARAIKEKKVQILFYNPRDFAKP
                     TKKQNTKEKPYFRVDDKPYGGGPGMVMQAEPVLKAIEAAMKKLKLKAKSYLSAGRGSR
                     AMSRDKLKARLIWLTPSGKQFDTKYARNIAQKYEHVIIVCGRYEGIDERVKKIYKMED
                     VSVGPFILTGGELPAMIIADSVSRQIKGVLGNFDSREESRMSSSEVYTRPETLRWKSK
                     KYTVPRILLSGNHAEIDKWRADRRLKKDHKSSRNSK"
     gene            20719..23949
                     /locus_tag="VI981_03820"
     CDS             20719..23949
                     /locus_tag="VI981_03820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY4479454.1"
                     /translation="MSDEKKPKRILVEGKVSGAGRVVKSFASYREALIPSPNLVESQT
                     DSFNWLIEKGLKEVFGEFSSVSDYSGKKFDLKFTDFKLSEPKYDEFYAKDNKLSYEAQ
                     LKATVELKNKMLGTSKEQEIFLSDFPLMTSHGTFIVSGIERVIVPQLARSFGVFFTDT
                     EIKGRKQFGAKIIPARGVWIEIETDSDGSLYVRIDRKRKFSVTSLLRVFGVKNDAEMF
                     ALFSKNPHAKQQLLASLTKDHAKTPDESFIEVHKRLRDGDLATVDNARDFIRSIFSEE
                     RYDISKVGRFRFNKRFGKKMEGKEVERKIVNLDDLITIVDHIITLNNTQGALADDIDH
                     LGSRRVRYVGELLQQKIRVGMSQIKRNIQDRMSTVEPDVTQPLSFISPKPLQARLKEF
                     FTTNQLSQFMQQENVLTELEHLRTLSALGPGGLTRERAGFEVRDVHPSHYGRLCPIHT
                     PEGPNIGLILRLATYARINDFGMIETPYARVKNGKVSDEVIYLNALEEENYAIAHAAT
                     KVDETKKIADSLVEVRINGRPSVVSREKVDYMDVGTAQAFSIATAMIPFLNHDDANRA
                     LMGSNMQKQATPCIIPEAPLVATGIEEKAARDSGRLVVASEEGTISGVDAKEVRVKAT
                     RTGKDKKYSLVSFSRTNGFTVFHQRPIVDVGQKVKKGDVLADTSSTDNGQIALGQNIL
                     VAFMSWSGANYEDAIILSERLVRDSKFTSIHIEEFVVNVRDTKLGPEVTTHDIPNVGE
                     AKLKNLDEDGVVRIGAEVRPGDILVGKITPKGETQLTPEERLLRSLFGEKARDVKDTS
                     KRMEGGKRGRIISVKIFSREKGDKLESGIIKRIHVEVAQLRNVTVGDKLAGRHGNKGV
                     ISKILPVEEMPFMEDGTPIDVILTPLGVPSRMNLGQILELHLGLAAHTLNYQAIVPPF
                     SGATDAEIKEELEKAGYARNGKMKLFDGRTGVKFDQDIAVGFMYILKLHHMVEDKIHM
                     RSIGPYSLITQQPLGGKAQGGGQRFGEMEVWALLGHGVAHTLREMLTIKSDDIVGRSA
                     AFDSLVKGEKINQSNIPASFNVLLNNLRGLALDVEVKKDASVIREK"
     gene            23955..>27090
                     /gene="rpoC"
                     /locus_tag="VI981_03825"
     CDS             23955..>27090
                     /gene="rpoC"
                     /locus_tag="VI981_03825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY4479455.1"
                     /translation="MNHRTKNINVNDFDSITLKLASPQRIKEWSYGEVTKPETINYRT
                     QRSEKSGLFDEKIFGPDRDYECYCGKYRGIRYKGIVCEKCGVEITRSIVRRERMGHID
                     LATPVSHIWFLRSMPSRIGLVLGMSTADLEKVIYFAGYIITHVNEDEREQLLKDLDSE
                     YKAKVKNLQEEKSKDAIKELLLNAKKEIDMIQEGVVLDEVQYHNYSVKYGTLFEAEIG
                     AEAVYKILKELDMDKLLERLVREKDKAGSIEREKINKRISLLKSMISAAIRPEWMFLT
                     SIPVIPPALRPMVPLDGGRYATSDVNDLYRRVINRNNRLRKLKEINAPDVILRNEKRI
                     LQEAVDALIDNSIRHGNSNAGALNQMQRRPLKSLSDNLKGKRGLFRQNLLGKRVDYSG
                     RSVIVVGPDLKLNQCGLPKHMALELFRPFVIAQLLKKEYAYNIRGANRLIEEDLPEVW
                     AILEEVIADKHVLLNRAPTLHRLGIQAFRPVLIEGNAIQIHPLVCTAFNADFDGDQMA
                     VHVPLSDEAQIEAREIMSADRNILKPGNGEPTVSGKLLDIILGCYWMTKETTGGLGEG
                     KFFPSPNAAITAFDFGVVEFRAKIKVLATDTVKYKQFDSKIFETTVGRLLFNSVLPSD
                     YPYINKEIARKQMAALIDDLIDKYGIDNVSPMLDKIKGFGFKYATHSGTTWGIDDIQE
                     PIAKDGVIKEAQKKAAEVDEHYDLGFLSKEERLRKTIEIWQACKAEVEKLIPGTLDKN
                     SSVYDMVYSGARGSFSQITQMAGMKGLIANTAGETIEFPILSSSKRGLTPIEYFITTH
                     GSRKGLTDTALNTARAGYLTRRLFDVAQDAIITEEDCGTKESILIRKETASGMEVSIV
                     KNIKGRVLGEDVTDKKGKVLFKRGHLITKEDGDNIEAAGVTEVKARSPLVCKTGNGIC
                     IKCYGTDLGKNVLVELGEAVGTVAAQAIGEPGTQLTMRTFHAGGTASVGGDITQGLPR
                     VEEVFEKRKPKNPAIVSKVDGVVTAVKDLGKEKIIVVLPEVDSKSKTKKKAEVEYTVN
                     YKRMALVKVGGKVKK"
BASE COUNT         7566 a         5884 c         6573 g         7067 t
ORIGIN      
        1 aaagaaggcg taaaaattca ggatatcgca gatgagcgtc taacgtttgt aagcgaaaca
       61 cacaccggta atattaacgc cggttataaa ggcagtgctc catataatcc ctcaagccgc
      121 atggttaagt ggcatacgac ttttttccga gcgggagatg ccggcacggt gacatggaga
      181 gcaaaaatcg gcgtgacgga agaagagtgc agtgttgttg aaatcccaaa cagtgctcgt
      241 attggagcga ttcttggaga cgacgaagat gaatgggtgt ggacatatag caatactgtt
      301 aaaacaactg taggcagtac ctgcgaatgt agacttgtta tctcaaaatt agttaacatc
      361 gcgaacgctg gtaatggaga tgtacttacc tatactgtga acttcaaaaa tgagggcgat
      421 gccgactgca cgggcggcgg agtcaaaatt gaagacacgc ttgattcgca tcttgtgtac
      481 ttgagtgagg ctcacgcttc aaacgtaacc ccaggctatg gcagcagagg tgtatacacg
      541 gcttcggaca gaattgtgcg tttcaatgcg ggcacgctca atccccaaga atccggctgg
      601 ttctcgttta atgtccgcgt ggctgatgct ccgtgttccg gatacgatgt tccaaatagg
      661 gcacgagcga gcgcggagga attaaattgg aactggatcg aaagtaatac agtctcgacg
      721 catattgagt caaatgattg caatcagtcc gttctttatg gcagctgcga agccagtccg
      781 acttcgggac gagttggaga tgggttcgta tttagcgcaa acgcgacggg cggaaacggc
      841 gttttctcat attcatggac gggaaccgac ggactccgcg gctcgtcacg caacgtcaat
      901 aagacatata caagcgctgg tctcaaaaat gctactgtca gcataacgtc agggaatacc
      961 atcataaata aaacttgtac ggtgaatata caggaaccgc cagttgttgg cggaaacttc
     1021 agtgctgttt gctccgtcaa ccagacgtcg ggagttcagg gctcactatt tacgttcaac
     1081 gcaattccta caggtggaac cggatattat acatacagct ggacaggaga tgataatctt
     1141 attggcacat cgcaatcagt acagaagggc tatatgacag atggtcgcaa agacgcaact
     1201 gtcgccgttc ggtcgggaca cgagacaatt actcgaacct gttctgtcga tgtcacccac
     1261 acgacaacgg gatgttcaac gtgcggcggc atcggagtcg atcagccggt agctaaactc
     1321 acaagctcta aagtgccgac acagcctgaa gccgcttttg tgttcttaag ccagattccg
     1381 tataccggaa tcgatacata tggagaaatc tttctctatt ttctaggagt ggcgctctgg
     1441 agcgtcttgg ctgcaggata cgtggtgtat cgcaaaacta gaaaacttcc catcctcaaa
     1501 ttttcgagaa ccgaggcata cgcgagtggc tccatggcaa cagtaccgat gaatcttccg
     1561 acagaggctc gtgcaccgca gatccgcgcc gaactcacgc tcatcgagaa actcgaacag
     1621 tacgcacgag aaaattccgc actcgtttct ctcggcggac tgaacaaagt cattgaactt
     1681 tcaggaagtg acgaagtgaa agcaaaagaa atactgaaat acgtcgttga aagctataag
     1741 gcgaacgata cctatgactg ggtcacgctt gatgaggcca aggtggaaca aagccttgct
     1801 gtcaatgagt ctccattctc gcgttttgtg agcgctttgg agcaaaacga cgtagatcgc
     1861 atccgcatgt ttcttaaaga agtcgaaaaa accggtatga cgctcaagca gtttgccgag
     1921 agtgttgttc gtgaactcga tgaagtttac cgacatcgaa ccggagtaaa gaattctgca
     1981 aatatgcata ttgtagagcg gctaaaagag tggcgtcacg agcatattca cgcagtaaaa
     2041 actgcgatgg aaaattcact gaattccgcg cttcatacgc cgcatgtggc agtaaaactt
     2101 ggcattttgc gtgccgcaga aatcatcgcc taaagaacac tcgaataatt ctcttttaaa
     2161 acgcgcattc cgcctcaggc ggatactatg ctcggcccga cggcctgcgc aatgtttaaa
     2221 aatagaaata ttctcgcttg gatggtaagg ggtaaattct ctttcgcaag tctttttata
     2281 atgatatgcg tccgcttagt tggaaggtaa ccctagcatt taccagcgat cgctggtaaa
     2341 tgctagggtt acgcagcctg ctcgtttctc ataccgacgc gaatactgtc aagatataca
     2401 gcgatagaat caaaaacctc atcagagcga atagcgagca ttgtttcgtc ggaaactttc
     2461 ggattagact cgattatttc gttgattttc aaaagagtat tttcatatat ccttgcaaga
     2521 tccgttttgc cgatatgttc ggcgtattcc ctgaataccc caagttgtat gcgaatttcc
     2581 tcgagtatct tgtgctgcat cggatgaatt gcaagctcct tattttttcc tgacatccat
     2641 gtatcgatta cttcatatac ggaagctttg tccgctccga tcacgacacg aggaatatta
     2701 ctcatgtgac cccggatgct taagtggtcc gactggaaat atttaatttc ggcaagcctg
     2761 ttgtcacgaa tctctgtcaa aattcgttct aggttttcag caaggcgagt actcgttgtt
     2821 acatctatgc cgagcgccaa atgagaagca cccgaacctt cctggatttc aactatcgaa
     2881 tcagtcccat ttttaatgtc gtcgaatctc gatgtctgta aaatcgtcgc attttcaccg
     2941 aaccagttat gttggtctga ctcatacaca atagcttcga ggcaacgccc gaccatcatg
     3001 aagtgtttac gttccggatg ctgacgctcg tcttctctaa atttcgcctc catctcccca
     3061 acccacttta tgtccgcgtc aacatcctta taaatatcgg cgaagtctct aggatcaatg
     3121 ggatttgcca acactttctc ggccctctca tatgcagcag gaacgtttac aaatttctca
     3181 ttttcaccga taaaagcttc gggatgtctc atttcaataa attattttta aaaaaatcaa
     3241 cgagagtgtc ttgcttaagt acagtgctct ttccggtgtc gcgattttcg agtgtgaccg
     3301 tatcgtcagc ctgttccttg tcaccgatta ctattgcgta tggaattttc tgcattttaa
     3361 aaagtctgat tctttttcct acagtgtcat ttgagttgaa catttctgtc ctaatgtcgc
     3421 catcattgag gagtacctca catttgtttg cgtactcatg gtgcttttca ctgaccggaa
     3481 caatcgccac ttgtatcgga gcgagccaat agggaaatgc gcctgcataa tgctcgataa
     3541 gtatgcccat aaaccgctcg agcgatccag agattgctct gtggatgacg acaggtcgtt
     3601 ctttcccaga tctatcattt gtaaatgaaa gatcaaacct ctccggcagg ttaaaatcgc
     3661 actgaatagt ggcaagctgc cattctcgtc caatcgaatc tttgaatatg aaatcgagct
     3721 tgggaccata aaacgctgct tcacctacga ctcgcgtgta attgagatta ttggccttgg
     3781 cggcatcgtc aagagcatcc tcggctttct tccatgaatc ttcagcgcca agatatttac
     3841 tcttatcttc tcctcgaacg gaaagactga cccagtagtc attcaaactg aatcgatcgt
     3901 aaaattctct gatcacttgg acgatcgttg atacctcctc ttttatttga tcaactcggc
     3961 aaaaaagatg cccatcatct tgcgtgatag ctcggaccct ggtcaaccca cccaactgtc
     4021 ctgacttttc gtcacggtat actgtggcgt gctcaaaata gcgaacaggc aaatcacgat
     4081 aactccactg attgtctgca aaaatctgca tgtggtgcgg gcaattcatg ggcttcatga
     4141 tgaatttctc ttcttttccc tgaacccgaa acaattcatc tccaaacttc gccgcatgac
     4201 ccgaggttac gtataactct tccttggcaa tgtgcggcgt ccaaactcgt gaatagcccc
     4261 gcttacggtg caaatcccat aggaaatctt ctatggcttt gcggataatc atgccttttg
     4321 gttgcataag cggaagtcct gatccgacga gaggagagag tgtaaaaaga cccaactctc
     4381 tcccaagctt tttatgatct cgcttttttg cttcctcgag ctgtgtttca tatttttgca
     4441 gttcctcctt tttgtgaaag gcgagcccgt atattcgagt aagcatcggc cgtgatgcgt
     4501 cacctttcca atacgctccc gcgatgtgag aaagcttgaa tgagtcaatg gcaatggaac
     4561 gcatctcgtc aacgtgtccg ccacggcaca aatctgtaaa agcgccggac gtgtataggg
     4621 taattttctc atttctggat tttatatcat tgagtagttc taatttatat gagttatctt
     4681 tgtatatttc ccgcgcttct gcttcgcgaa cttctttgta tccaaactcc ctccatgact
     4741 tatatatttc tcgcattttt ttttcgattt ttgggaggtc ggcgtcgcta atcttttccg
     4801 acccaaaatc gaaatcatag taaaaaccgt tttctatggc agggccgata gtatttttcg
     4861 cagtaggcca tagctcaagc acggccgccg ccagaaggtg cgcgagtgag tgccgaatgt
     4921 ttccgatttc gttgactgaa gccatggcag aaatcatagc atttttgaca atgaagacaa
     4981 caaaaaaacg cctctagagg cgtccaaaaa tgtgaaggag aacgatgaca agagagaata
     5041 cgagacagaa cgaaacggtc ttcggaatct cctttgtgtt tgggaggcct ttgcgaattg
     5101 gagcaggaaa agatacgtac agtagaagac aggagagcag aagcgcgctc gcggtgagtg
     5161 aggtgaagag ccctcgtgtg tctggtccta ccacaagaaa gagcgagatg gacagaagca
     5221 taagaataaa ccccgtaata aggcagacaa caaaaaggca gagagaaacg agcagaacga
     5281 tcattctatg catagctgga gaatactctt gcacaaaacg tcaaaaaaac aagaaaaaaa
     5341 gagaaccctg cggtgccgtg agggcactcg cagggttcgg ttggagacac taccgtgtgt
     5401 aggaacggcc tacaccgtcg caggtcgcag gaggggtagt gaatttttct tgaggtaaca
     5461 agcctcaaga acggtatcaa ccaaaccgtg tgcatgtcga ccgagtgata gcataccaca
     5521 aaacggctag gactttaaag ctcctttatt ttttcagcga tgatgctttg gcggccattg
     5581 cgctctgaaa aacgaccttt aattgcaatg catttctcga gttgtagcat gcttttattt
     5641 tcttcataca ctcgaggaaa aatgacggtt tcgatatttc ccgtaaaatc cgctagagtg
     5701 agaaacatca tcttgtctcc cttttttgtc agaagatctt tggcctcttc aataatgccc
     5761 gcgaccacgg tcatcatgcc ttccttcatt tgttccttta tttttttgat tggcatatcg
     5821 tgtttatcga tcttgtcctt aaatttgtcg agcggatggc ctgaaatata gagacccaac
     5881 agctcctttt cccaggcaag cttattacct tgactagcag gttcggcttg ccgcagtgtg
     5941 agcgtgttgg cgtcaggcgc agtcgcaccg aaaagagaat tctgattgtc gttttcgttc
     6001 gtcttttcct tattgtatgc aagcaggtct tcgatattcg caagcataat gcctcgttcg
     6061 ccaagttgat cgagtgctcc gcatttaatg agtgcctcga gtgatttttt gtttaagctg
     6121 cgatccttga tgcggtcgag aaagttcgcg agcgagccaa attttccatt cttttttctc
     6181 tcggcaatta tcgtatttgc aataccctcg ccaaaatttt ttatggtagt cagtccgaaa
     6241 cggatcatgt tttcttcttt cccatctttc cgaaccactg tgaatccgct gaagctcccg
     6301 ttaatatcgg gcgcaaggac gggaattttc attcgcttgc attcagcgat tatttcaccg
     6361 attttttcga cgtcaccgga atcagcggta agcacgctcg ccatataaac ggcagggaag
     6421 ttggctttca tatatgccgt ttgatatgcc acacgtccat aacttgccgc atgggcctta
     6481 ttaaatccat atgcggcaaa gggttcaatt agtttccaca attcgagagc gttggcttcc
     6541 gtcatgccgt ttttgacgag gccattggtg agcttttcct tttcggcctc catgactgat
     6601 ggaatttttt tgcccattgc ttttcgaagt ttgtccgcct caagccacga gtatcccgca
     6661 agattgaccg cgatcaaaag cacgtcatcc tgataggtga tgactccgta tgtttgtgaa
     6721 agaatatctg cgaggcggcc gtcaaggtag ctcacacgct ctgtattgtg cttacgctcg
     6781 atgtagagcg gtattgattc catgggtccg ggccggtaga gcgccaccat agcgttaata
     6841 tcaaaaattg tggtagggcg aagctgcttt aagtacgccg tcatgcccgc gccgtttaac
     6901 tgaaaaagtc caatcgtctc tcccttcgcg agcaagctaa atgtcttttt gtcattgagt
     6961 ggaatgtgtt cgatttctac gtcgacattg tagacctttt ttgtcacttt cacggaatcg
     7021 gaaagaatgg aaagattttt tattccaagg aaatcaaact taagaaggcc tgcgtcgtcg
     7081 acggcatgca tgtcatattg agtgataatt tttccctcac cttttgggtc aaattgcaat
     7141 ggtacgtagt tggtaagtgc gcttggtgca atcacaacgc ctgcggcgtg aacagaaatg
     7201 tgtcgagcac aaccttcaat tttctttgcc atgtcaacga tatccttcac ctctttttcc
     7261 gccttatata ttttttttag atcagggact tcatcgagcg cgcgatttat ggtcatcggg
     7321 aaaccctgtg cacccatggg aataagtttt gcaatgcggt ctccgagcga atagggatat
     7381 ccgagcgctc gagcagtgtc tcgcactgat ccgcgggcca tcatggtgcc aaaggttcct
     7441 atttgagcaa cgcggtcttt gccgtatttc tgtttcgcgt attcaataac ttcatcgcgg
     7501 cggttgtctg cgtaatccat gtcgatatca ggcgcacttg gacgttccgg attgagaaat
     7561 cgctcaaaag gcaatttata ttcaagcggg tttacgttcg tgatgccagc aagatacgtg
     7621 acgagcgatc ctgcgaccga gcctctaatc gtggttaaaa tgtcacgctc atgggcgaat
     7681 cgaaggagat cggatacaac caaaaaataa ggcgaatagc ctttattgaa gatcactcct
     7741 agctcatatt cgaccctctt tgtaatttcc gcagtctcct gtagttctcg tttcttgatg
     7801 ccttcataga cgaggcggcg taattcgtcg ttgtaggaaa gtccgctctc gacgacgtaa
     7861 tttggaaaca cccatttacc gagctccaat tcgacattgc atttttccga gatttcaaaa
     7921 gaattcgcaa ttgcggaggg catggagcga aaataacttt ccatttgttt tccggatgta
     7981 aatgagaaat tctcctccgt gccgctcgaa tctcgacgct ctataaagtc tgcgtttgtc
     8041 tgaaccgaca taagtgtctc gcgtgcccgt ctgtcttccg gatcgagata atacacatca
     8101 tgagcagcca caagaggcgt ggtggttgct tcaccaagcc gttcaacttt cttcatgaga
     8161 tctgcatgac cttcgatttc aggatgatgg gagatctcga gatacacatt ttcagccccg
     8221 aagattgatt tgtatttgcc aactatttcg gcggcttttg aagggtccga gctcttcaat
     8281 gactgtgatg tctcgccgct aaaagaggga atgatggcga gcaaaccact tttgtatttt
     8341 tccatgagct cgtcatctat ccgcggtttg tagtagaacc cttcgaggta cgagtcggta
     8401 accatcttga tgagattttt gtaaccctcg ttatttcggg ccagaatgac aagacgcgaa
     8461 cgccggttgt ctatgcctgc ctgcttgtcg tggcgggatc gtagggctac ataagcatcc
     8521 acaccgataa ttggtttaat cccaaaatct ttgcactttt tatagaactc tattgctcca
     8581 tataaatttc cgctgtcggt cagtgcgagc gccgacattt cattatcctt tgcgcgtttt
     8641 accaaatctg ggatttgggg taaggcattt agcaggctgt aatggctgtg agtatgtaga
     8701 tgaataaact tgctcattgc gatatgaaga cttgccccgt agtagacgcg gagcggctta
     8761 cttcggggtg gataaacttt gacatgtgat caaattatag ctcatacgca gatacgtcgt
     8821 ataatatgca caatgctttc agagactgcg caaaggtgcg ccatcaaata tcttatcggt
     8881 atagacgaag tcggacgcgg gccgctcgcc gggcctttta cagtaggagc cgtagcgatt
     8941 ccttttggtt cgaaactgta tttgggcgcc gtcaaagact caaaacagct cctacccgag
     9001 gatagggacc gactttttag gaaaataaat gcattaagaa aagatgggaa attgcgccat
     9061 tctatttcgc atattgcgcc gcacgtactt gataaaattg gtctcactaa atctgcacac
     9121 agcgcagtag cgaggtctct taaaaaactc aaagtaaagc ctgatgaatg tttagttttg
     9181 ctagatggtg gattaagggc accgaatgaa tttctcaatc aaagaacagt cataaaaggg
     9241 gatgaaaaaa taaaaatcat tgcgcttgca tcaattgtgg cgaaagtgac gcgagatagg
     9301 actctcatcc ggctctccaa aaaatatccg gaatatgatt tccatgttca taaaggttac
     9361 ggcaccaaac gtcactacga gagactccga ctttacgggc catgcgaaat tcaccgaaaa
     9421 ttatttttac ggaagttttt tgagagctgc tccacggttt aacgtcgcgt tgaaaccatt
     9481 gccttttttg gtaactaggc tatactaagc cgcactatgg tcgttcgaat gcgtcacaac
     9541 cggtcacaga ccggaaacac aagaagccac cacgccttaa aaccggcacg gctttcaaaa
     9601 tgccaggact gcggtcatat gcatttgagg cattcaatgt gccaaaattg cggcaaatat
     9661 atgggagtgg ttgtcgttga tgttcacgcc aaaatggaaa agaaagaggc ccgacgaaaa
     9721 gctagactga aagagcgggg ggaagaagta aaaaaagcag aaaccgaaac agcggacgtt
     9781 aaaaaatcat aaagttatag cagtcaaggg tactaatccc ttgaaacgtc cgccgcggcg
     9841 ttttaaaata cacaggtata tagtacatat ggcaaacagg caccttgcac gatcgacagt
     9901 cctgcagaca ttatttgaat gggattttgc gggaaagaag gatactgacg cacaagaaat
     9961 tcttatgcgc aacgctaagg agtttgcttc tggcattggt gatacttcgt tcctacagca
    10021 cctcctcggc aatgtcttgg gacagcggga gaaactggac gaaatcataa caaaggctgc
    10081 tcccgagtgg ccgcttgaaa aaatttctgt agttgaccgc aacattctaa gaatggggct
    10141 gtttgagtta ttgttttccg atagaaacga ggtacccgcc aaagtggcca ttaatgaggc
    10201 gatagaactt gccaaaacat ttggaggaga aaacagtgga cgttttatca acggcgtttt
    10261 agggtcagtt tataaggaaa tcggcgaacc tggaaaagat tctccggcgc ggccgcgcaa
    10321 aaaagtggtt gatctgaaag atatgccgat cgaaaaattg gcgggggccg tcgtgtatgc
    10381 aagaagagag gaagacattt ttcttgcact tgtgcacgat atatttggac actggactct
    10441 ttcaaagggg catttaaacg aggctgaaat cgagaaagag ggtgtagtgc gggttataaa
    10501 ggaggaaatt aaccttgacc tcgaagttcg tgaggaactc ggacgtaacg aatacatcgc
    10561 ctctgatccg gaaaaaggga aaattcgaaa gcaagtcatt tattttctgg ccgagagtcc
    10621 gttcgtggac attgtactcg cgaaatcggg cgggctagac gatgcccgat ggtttaaact
    10681 ttcagatatt cttgacctga atttttataa tgatatttta ccgcttgtta ccaaggccgt
    10741 gcagatactt ttgagcagaa gtgctgaaga gacagtcatg tccaggaaaa caaagtaaac
    10801 cccgttagaa gctcacaagg ggcattaaac cccgtgttcg ctgaaatcaa attgcgtttt
    10861 gcttctaacg gggtaaaggg cggcaatttg tctttttttc ataaataagc ataattgact
    10921 cattatcagt gaatataagg tattgcctcg agccgtcttg cgactgaaga ctattcgcgg
    10981 aaatacctca aaatatatgg attatccgga ttttgagaag catattggtg ttagtttcaa
    11041 aaataaagca ctgcttttac aggcatttac gcatcggtcg tatctcaatg aaaataagcg
    11101 taatagctta gagcacaacg agcggctcga gtttttgggt gatgccgtgc tcgagcttgt
    11161 tattaccgtt tttttattca ataaatatcc cgacaagcca gaaggggaat taacttcata
    11221 tcgagcggcg gtcgtaaatg cgacgactct ttcaaatgtg gcaaagagtc ttttgatgga
    11281 gccgcttctc cggctttcac acggtgagag taaagacaca ggaaaggcac gacaaattat
    11341 tctggcaaac acgatcgaag catttattgg ggcggtattt cttgatcaag gatatggaac
    11401 agtggagaaa tttatactga aacatattgc gtcgctctta gaaaaaatcg ttgaaaaagg
    11461 agcgtggatt gacgccaaaa gctatttcca agagcaggcg caggaacaag tcggaattac
    11521 accagcgtac agagtggtgc gagagaacgg accagaccat gataagaaat tcataatcgg
    11581 ggtttatttg cgcgatgaaa aagtggcgga aggagagggg aaatcgaagc aggaggctga
    11641 acaaatggcg gctgaagccg gactcaaagc taaaaagtgg aagtgataat cttcttctga
    11701 caaaaaactc ggctgtctct gctgtttttc aaatacacta actttcacga tcgcgaaagt
    11761 tagtgtattt gatgtttgac aaatgactga tctggagata gtgtttgtta gggtccgctc
    11821 gcgatacgtc aaaggcgcgc ataacagcac ataaaaacta aaatgaaagg tgacttatgg
    11881 atacgtcctt cgtcatgttg agactcaatg aagttgccga acaacttaaa ccgatcgcgg
    11941 gctatcttgg cttcagtcgg ctcggcgaga tcatggattt aaagaccccg atcggacata
    12001 cgcaaacgag gttatttagg ctttttcccc gacaagttta cgttccgctc gacgcactct
    12061 gctttgactc tcgggttcgg cgactcattg attttgcgaa gcccgatttc tccgcaccaa
    12121 ttacaacagt tgaccagctt gtaattgcgc aagatctgcg ttggttcaga tggaaaaccg
    12181 tttaccgact tgttagcgaa acctacgctg aagcgttgag ctctactatg cagctcgtcg
    12241 tggacagaag ttctgttccc gaatataccg tatggctcga gcgtctagat aaggcaatcg
    12301 atgagtggat cgttgaatcc atgggtcgac gaagcgaaat ctgcaccaag ctcgagatgg
    12361 ttcgacggaa acactttgaa actgcttaac ggcagttttt tacttgtgcg taatacagat
    12421 attgccatag cttaaattca gtggtagtgt gattgtgggt gttttgacgt aagtccgagt
    12481 acaagtaagt tcaactcaca aaagggccgc ggcccggaaa ggtatacagc ttatgaaacc
    12541 gtttaatagc attgcgcacg aacttcagtc atgtaaagac gcgctgcgat ttaacaaggt
    12601 cagtgactat cttaaccgcc aagaacacga tgtgccgata cagatcgtgt ctgggcacct
    12661 cttgttaccg gtcggaatag gcctcgactc gcccctcaaa atcctctata ccgtttcaga
    12721 cgactatgag ggggactgca aacagttttg tcgcgaagag ctcggatata cgcgtgcgta
    12781 tcgaccttgg atacggtgga ggacggtcta cgaacgggcg agtctcccgc acacctgtta
    12841 tttcgggcgg gagtccacga tccatgtcgc tgacatgtat caagttttag ccattttttc
    12901 aaatcctgac cggcgctccg agttggaagc aagtgttgtt gagtggattg cagagtccgt
    12961 taagcggcgt ctggctctca tcgaggctct gaaggaatcg agtgatcgtc tgacgactgg
    13021 gacatagcat ttccgtaagc tccgcacgcg cggcgctttt tttatacccg ctataacgtt
    13081 ataattctag tcacctgtga agaaatttac ttacaagacc ataatgtttt taatgggatg
    13141 ctgaaatcac tggaactatc ggggttcaaa tcgttcgcca aaaaaactga cctggatttt
    13201 aagaccgcta tcacggcgat cgtcggcccc aatgggtccg gcaagtcaaa tgtggcggag
    13261 gcttttcgct ttgttttagg cgagcagtcg atcaaaagca tgcgcggcaa gaaaggagaa
    13321 gacctaatat ttaacggttc gaataagata cccaaaatga atcgcggggg cgcgaaagtc
    13381 atattcgaca acagaaagcg aattctcaat attgatttcg atgaggtttc catcgagcgt
    13441 gttgttcacc gcgatggtat aaacgaatat tctataaacg gaagtcaggt gcgcctcaaa
    13501 gacataatcg agcttttggc gggggcgcat atcggtgcca cggggcatca tatcatttca
    13561 cagggtgaag cggataaaat tttaaacgcc aatgttcgtg acagacggga aatgatcgaa
    13621 gatgcgctcg gactaaaaat ttatcaatac aaaaggcagg aatcagcgcg gaaacttgaa
    13681 aaaacagctg aaaatatcgc ccaagtggaa tcgcttcggc gggaaattgc gccacacctc
    13741 aaatttttac ggaagcagat tgagaaactt gcgcgcgccg aagtgatgaa acaagagcta
    13801 gtgcttttgt attgccaata ttttaaaaga gaaaatattt atttaacatc cgagaagaat
    13861 tttattgaaa ccgaaaaacg gaagcctgtg gcggagttgc gcgcgcttga ggaagaactc
    13921 acccatgctc ggaaacagct cgagggtatg tcagacgcaa aaatggcgac acaactgttc
    13981 gagctcgagg ggaagattgc cggtgcccgc catgcaaaag acacgctcac aagagatttc
    14041 ggcagggtag aaggggaaat agctggcgtc gagcgagctc gaaaaaggga agaagatgcc
    14101 gcagcggagc acgacctgca tactctgtcg tttaaggaat ttgagtctct tgttgaatct
    14161 atatttgaat cgatacatga ggctgaaaag acagatgcgc tcgataaaat taaaagcctc
    14221 ctcgctcatg ttcgggaatc aataaaaaaa ttcatcgaga agaatcgcgc gtcaagccac
    14281 cgaaaaaacg gcgagatatt caaaaccgaa ctcgaacgac tggggggtga gaaaactgca
    14341 ttagagcgtg acctatctcg cgctgtagat acggaacgtt cgcatgtaaa ggaatatgaa
    14401 tccataaaaa gcaatatcga aatggagcgt cggggcgggc tggaggcaga aaaaaacatt
    14461 ttaaggataa tgactcggca gaacgaaata aattcggcgt atgcgagtgt taaggcgagg
    14521 gaagagcgac atcatatcga gctcgcggat tttaatcgcg accttacgga agcgggccat
    14581 attgccggac acgacgccat taattttgaa gataccgtca ttgtgcgaca ggataagacg
    14641 tcgataggtg agcgtgagat agcggaggag ccacgagaca gtcaacgtga gcgacgcaga
    14701 gtcattgagc gaataaaaat caggcttgag gaagcaggat tggcgggtag cgcggacctc
    14761 ataaaagaat tcaaagaatc ggaagaacgt gaccagtttt taggcaagga aatcgaagat
    14821 ctgactaagt cgtcggtagc tctccaggcg cttattgatg aactacagca aaaaatcgat
    14881 tcagagttta aagaagggat caaaaaaatc aacgctcaat tccaggattt cttcgccctt
    14941 atgttcggag gaggttctgc ttcccttgat gttgtagagg aaatccgaaa acgcgcgagt
    15001 gcaatcggtg agatagatgc cgatggcata gatgaattga gtgtgaccga tgaaaccgaa
    15061 gttccggaaa taggagtgga aataaaagta cacttgccac acaaaaaaat acgaggactt
    15121 gaaatgctct ccggcggtga aagggcactt acttcaatcg cgcttatttt tgccatgtcc
    15181 caagtaaatc cgccgccgtt tttggtactt gacgagacgg acgctgcgct tgacgaagca
    15241 aattcaagaa agtacggcga catgatagaa aatctggcca aatattcgca gcttatttta
    15301 attacgcaca atcgggaaac gatgtcgcgg gcggggattc tctacggcgt aaccatggga
    15361 ggggatggga cctcgaaact tctctcaatt gcgtttgaag aggcggtgaa tgtttcaaag
    15421 tagagggcgc tcggatagtt tgttcgaaag gtgcacggat atccgccagc tggcggattc
    15481 ctcgagctcg cgccgtcgcg ctcgctaggc actttcggcc aactaccctc gctccgacga
    15541 tgggaaaaaa agaaaaaacg aggcagtgat gcgctcgttt tatagagatg gaggccgaag
    15601 cgacgaaagt gtcgcaccgg cctcggagta catgagagtc ccagcccggg atctcgagca
    15661 agctcctcct ttccgccatt cggaagggtg cgtcctgata agaagaaccc aaccaatgat
    15721 gtattatact tcctttcttt ttgagtaaaa agcacctcta acatgtaatg cgacagccta
    15781 catgaaaccg cgctctgcga gttggaaatc cagcgttctt cgttccatgt cggcaccggc
    15841 aactctgaag cggattttat cgccgagaga atatgttttt tttgcttttt caccaacgac
    15901 ccgatagttt ttctgatcca gtgcgtaaaa gtcatcgccc aaatcacgaa gctttatcat
    15961 gccttcacat tttgtttcca cctcctcgac ataaattccc cattcggtaa ctcctgaaat
    16021 gattccgttg aattcctcgc cgatgtgttc tttcatgtac tcaacctgct tgtatttaat
    16081 agacgcacgt tcggcctcgg cggctaaaac ttctctttca ctcgattgga tcgccatttt
    16141 ataataaaac gccaactcgt gcttcggaat ctctcttccg tttagatatt caaatagcac
    16201 tctatggacg agaaggtcag gataacgacg gatgggcgat gtaaaatggg tataatgttc
    16261 aaaggcgagg ccgaagtgac cgatattgct tgtcgcgtaa atggctttgg ccatagaccg
    16321 caatgttgct gtctttacaa gagattcctc gggttttccc gtaacatcat ttagtaattt
    16381 tttaatgctt tttgcggtaa gtttaccgtc gtgcgattcg agatcatacc caagtgcctt
    16441 tatgaaaagc tcgaggtcgg caagccgatc ctggtttggc gtatcgtgta ttcgataaat
    16501 aaaagcgcca ccgtcgatct ttcttttttt attttctttg acgatatatt ccgccacttc
    16561 gcggttggcc aaaagcatat agtcttcgac cagcatgtgc gtgtctaggc ggtctttttt
    16621 ataaacgcgg ataggtttgc cgttcgagtc aagctcgaat ttcacttctt ctttttcaaa
    16681 atcaattgct ccttttgcaa atttatgaac acgaagccta tgcgcgattg aatttaaaat
    16741 atgaagggat tcgaaatgac ccccggatct cgcgtccaat actttctggg cttcctcata
    16801 actgaaacgc ttagcggaat gaattatagt tttgccaaac caacgatcca gtattgtgcc
    16861 gctcatgtct attttgaaaa cgacggaaaa agtcaaccgg tcttcatcgg gcttaaggct
    16921 gcagatgccg ttcgaaagtg cttcgggtag catgggaatt gtgcggtcga caagatacac
    16981 tgaaaaagcg cgcttttgag cctctttatc cagctttgta tgcggccgaa tatagtgtga
    17041 tacgtctgca atatgcactc cgatctcata gatatttgtt ccgacggatc gaatcgatat
    17101 tgcgtcgtca aaatccttgg cgtcgacggg gtcaatggta aacgttgtaa cgtccctgaa
    17161 gtttctgcgc gtgttgattt caacctgtgg aatgggaaga agtgttttag caatgtgctc
    17221 tgcctcacgc tctacgtcag cgtggaatgt cgtttcgaat cctttgtcat agatgatcga
    17281 ctgcatttcg acgtcatgaa gtcccttttt gccaagaact ttaatgagct ttccttctgg
    17341 ccccttttta tcccatctcg aaagagacac gagcgctttt acgccgcttt ccatattttg
    17401 gagcttacca agactgatat ccttgtaaaa gcgcctgtcg tcgggcacaa gaaagtagct
    17461 gccctgttca cactcgagtg ttcccacgaa ctctgtttta gctcttttag tgacacgaac
    17521 aacctcacct tgaattcgct tattcttttt agttctcaag accttaacca gcacctcgtc
    17581 attgttcagt gccttgttta gaagctcggg ctgtatttca atatcttcgg cgtagtcgtc
    17641 gtgctcaaag taccccactc cctttgaggt cacattgatt gtcccctgta gcgaaacttc
    17701 tgtgtttttt tctttagccc tgtctctttt gtgtgttttg tccattgtga gagtctagca
    17761 aatatgcttt ttttatgcat cccgtggttg ctttagtaga cttgtcgaat cttgccattt
    17821 tattgttgct ctgttagtat ttcggtcacc tgtcccgtag taaattcaac tttgttatga
    17881 gacatgtgct gggcatacta tattctaata ttgccttaat taataaacat taaaatttta
    17941 ctaccgggat gctaaaaatc aggttacaga gagtgggtag gaaaaaacag ccgtattttc
    18001 ggtttgtttt gactgattca aaaaattcta cgaaaagcgg taaatttcat gaggtgctcg
    18061 gtttttttga ttaccgaaaa ggtggcgagc ataaaattga caaagcgaga gtcgtttttt
    18121 ggatgtcaaa aggtgcgcag ctaacgccga gtgtccacaa ttaccttgta ggggagaaaa
    18181 tcattgacgg aaagaagata aatgtattgc cacgtaaaag tccgccaaaa ccggaagagg
    18241 ccaaaaaaga agacaaaaaa acggaaaaag ctgcgaaagt aaaaaacgaa gaaactataa
    18301 ccgaggcccc taaacaagaa aatacagtcg aaagcactgc ttcttaagta gccagtctct
    18361 ggctcgagtg ccgagtcagg ttatcttgaa attccttttg ttttctgtac aatgtagtga
    18421 gcagattgaa aggaggaatc gcttttcgct gcctgattaa taggaatgtt catataaaca
    18481 catggaacaa gacgcacaat ttttagatta tgttataaag gctcttgtcg ataatccgaa
    18541 tgatgtcaaa attactcgga ctgttgacga gatgggagta ttgctgacgc tcactgtaaa
    18601 ccccgctgat atgggaaaga ttataggtcg aatgggtaac acggcaaagg ccatcagaac
    18661 actgcttcgg gtcgtaggga tgaaaaataa cgctcgagtt aaccttaaaa taaacgagcc
    18721 ggaaggagga actaaaccgg aacgagcttc aaagacggtt gacgaagcta tggaggacct
    18781 caagatatag aatttttact aaagcgctgc gcgagttgga aattgcaatc agcttagatc
    18841 gaaaattcaa aactcctaga aaccccgcat aagcggggtt tcttcgtcaa acagttgaat
    18901 tttcgatcgt cacactcctc aatttccaaa ctcgcttcgc taacccgtaa aaatctcata
    18961 tctttcggtc tacaaaagta ggactgcgta atgttcacaa taaattaata ctcacatgga
    19021 cgaaaatacc ctactaacgc tcaaagaaaa tatatttttt agtagacggc gaaaatggca
    19081 cgcatgtcgt cacatgtgta attgttgatg ggtctaggta cgtatggtat tctctcttat
    19141 ggctcggtgg attcgcgtct cgttgacaca aaactctaac cacaaatcca atgcatgaat
    19201 accgcaatgt tcgaaatcgt ggaagtggct cgcgttagat tggcctgctg caattgcgac
    19261 tcaccacttt gtgatgggac gtatgttgtc gagtgcagac aagatgatga cgtcggtctc
    19321 cttggcgggg tgtcatatca atgctgtggg caaactcgcg caggttttgt actttgcaac
    19381 tcgattcgcg aggcattaga ggtgattgta cacgcgcaag tggcgcatga aatcacactc
    19441 gttcctacgg ataaaattcc cgggtacaaa tgctaccgta attagttctt gagctgcgag
    19501 ttaaactcgc ggttttttct tttgtaggaa acccgagata caagtacgga ggaagttatt
    19561 ttcttgcctt tgattcgtta tattcgtatc atttccgcca taggcggatc cgcctatggc
    19621 ggacgcaagg taatacattc gtatatttgt atcgtgtagc gtagcgtatg acttttcaca
    19681 taatcacact gtttcctagt atgtttgact cgtatctcaa ggagtcgatt ttggcgcgcg
    19741 ccatcaagga aaagaaggta cagattcttt tctacaatcc ccgcgatttc gcaaaaccaa
    19801 caaaaaaaca gaacacgaaa gagaaaccat actttagagt tgatgataaa ccatatggcg
    19861 gaggtcccgg catggtgatg caggcagaac ctgtacttaa ggctatcgaa gccgcaatga
    19921 agaaattaaa gctaaaagct aaaagctatc tttcggcagg ccgaggatcc cgagcgatgt
    19981 cgagggataa gcttaaagct agattgattt ggctcactcc atctggaaaa cagtttgata
    20041 caaaatatgc tagaaatatc gcacagaagt acgagcatgt gattattgtc tgtgggcggt
    20101 atgagggaat tgatgagcgg gtaaaaaaaa tatacaaaat ggaagacgtt tcagtcgggc
    20161 cattcatcct gactggcggt gagttgccgg caatgattat tgctgactcg gtttctcgtc
    20221 aaataaaagg cgtgcttggt aattttgact cgagggaaga gagtagaatg tcgagtagtg
    20281 aggtctatac gcgtccggaa actttgcgct ggaaaagtaa aaaatacacg gttccgagga
    20341 tacttctatc tggcaatcat gccgagattg acaaatggcg tgcggaccgt cgactaaaaa
    20401 aggatcataa atcaagtcga aactcaaaat aacgcaattt catgtttgtg aaacccacgc
    20461 agtgctatat aatttgcaac ctatgtcaac acacgcgtga tttgactttt gcaacccatt
    20521 cgatataatc ccaaaatcac aattgcaaga ttgagtaacg gcaggtggca attaatggct
    20581 taaaagctta ataaatagcc agtatcttgc ttaaaatttc atactgacag agccgatcga
    20641 agagaggcct cggattgagg cgctctttag atgtctgtgg ataacttttt ggcggcgtac
    20701 tttttttaat aattacacat gagcgacgaa aaaaaaccaa aacgaatttt agttgagggc
    20761 aaggtaagcg gcgcggggcg tgtggttaaa agttttgcga gttatcgaga ggcacttatt
    20821 ccttcgccca atcttgttga gtctcaaact gattcgttta attggctgat tgaaaaggga
    20881 ctgaaagagg tgtttggaga gttttcatct gtcagcgatt attctggtaa aaaatttgac
    20941 ctcaagttca ccgactttaa actatcggaa ccgaagtacg atgagtttta tgccaaggac
    21001 aataaacttt cctacgaggc tcaactgaag gcgactgtgg agctaaaaaa caagatgctc
    21061 ggcacatcca aagagcagga gatttttctt tctgattttc ctttgatgac aagccatggc
    21121 acgtttattg tgagtggaat cgagcgcgtc atagttcccc agctcgcgcg gtcatttgga
    21181 gtttttttta ctgatacgga aatcaagggc cgaaagcaat ttggcgcgaa aataattcct
    21241 gcccgaggag tttggattga aattgaaacg gattccgatg gttcgttgta tgtgcgcatc
    21301 gaccgaaagc gaaaattttc agtgacgtct cttcttcgcg tgtttggggt aaaaaatgac
    21361 gcggaaatgt tcgctctttt ttcgaagaat ccacatgcga agcaacaact cttggcttcg
    21421 ctcacgaaag accatgcaaa aactccggat gaatcgttta ttgaagtaca taagcgcctt
    21481 cgcgacggag atcttgccac ggtcgacaac gcccgagatt tcatccgttc gatattcagt
    21541 gaggagcgct acgacatttc aaaagttggt cgcttccgct tcaataagcg atttggcaaa
    21601 aaaatggagg gtaaggaagt cgagcggaaa atcgtcaatc tcgatgatct cataactatc
    21661 gttgaccaca ttattacgct caataacact caaggtgcgc ttgccgacga tattgatcac
    21721 cttggatcga gacgtgttcg gtatgttgga gaactgcttc agcagaaaat tcgcgtcggt
    21781 atgtcgcaga tcaagagaaa tatacaagac agaatgtcga ccgtggagcc ggacgtcacc
    21841 caacctctaa gttttatctc tccaaagcca ctacaggcaa gacttaagga attctttacg
    21901 actaaccaac tttcgcaatt catgcagcag gaaaatgttc tgacggaact tgagcatttg
    21961 cgcacgctct cggctctcgg tcccggcggt cttactcgag aacgagcggg gtttgaagtg
    22021 cgcgacgtac acccctcgca ctacggaaga ctctgtccga tacacactcc ggagggtccg
    22081 aatatcggtc ttatcttgag gcttgccacg tatgcacgca tcaatgattt tgggatgata
    22141 gaaactccgt atgcgcgcgt aaaaaacgga aaagtttcgg acgaagtcat ctatctgaat
    22201 gcattggaag aagaaaatta tgctattgca catgccgcca caaaagtaga cgagactaaa
    22261 aagattgccg actctcttgt tgaggtgcgt atcaatggcc gtccgagtgt cgtgtcgcgt
    22321 gaaaaggttg actatatgga cgttggaacc gctcaagctt tttcaatcgc gacagcaatg
    22381 attccctttc tcaatcacga cgacgcaaac cgagcactca tgggttcaaa catgcagaag
    22441 caagcgacac catgcattat tcccgaggcg cctctagtgg cgactgggat cgaggagaaa
    22501 gcagcgcgtg attccggaag attggtcgtt gcgagcgagg agggaacaat atccggcgta
    22561 gacgcgaagg aagttcgcgt gaaggccacg cgaaccggca aggacaagaa atatagcctc
    22621 gtctcctttt ctcgaacgaa cggttttact gtgtttcatc agcgtccgat tgttgacgtc
    22681 ggtcaaaaag taaaaaaggg cgatgtcctt gctgatacat cttcaacaga caatggtcaa
    22741 atcgcgctag gtcagaatat tctagtggcg tttatgtcgt ggtcaggagc aaattatgaa
    22801 gacgcgatca tcttatcgga acgtctcgtt cgcgatagta aatttacttc aattcacatc
    22861 gaagagttcg ttgtaaatgt ccgtgacacg aaactgggac cggaggttac cacgcacgac
    22921 attccgaatg tcggcgaagc taaactaaaa aatcttgatg aagatggagt agtgcggatc
    22981 ggcgcggaag tgcgtccggg agatattttg gttggaaaaa ttacaccgaa aggtgagact
    23041 caactcactc cagaagagcg cttactccga agtttgttcg gcgaaaaagc gcgagatgta
    23101 aaagatacgt caaaacgcat ggaaggaggg aagcgcggtc gtattatttc agtaaaaata
    23161 ttttctcgtg aaaaaggaga taagttggag tcgggcatta taaagagaat tcacgttgag
    23221 gttgcccagc ttcgtaacgt caccgttgga gataaactcg ccggacgaca cggaaacaaa
    23281 ggtgttattt cgaaaattct gccagtagag gaaatgcctt ttatggagga cggaactccg
    23341 attgatgtca ttttgacgcc gcttggcgtg ccgtctcgta tgaatttggg tcaaattctc
    23401 gagcttcact tgggacttgc cgcgcataca ctgaactatc aagccatagt tcctccattt
    23461 tccggcgcca cagatgctga aattaaggaa gagctcgaaa aagcgggata tgcgcgaaat
    23521 ggaaaaatga aactttttga tgggcgaacc ggtgtgaagt ttgatcagga tatcgcggtg
    23581 ggtttcatgt atattttaaa acttcaccac atggttgaag acaagattca tatgcgctcc
    23641 attggcccat actcgctcat tacccagcag ccgctcggtg gcaaggcaca gggaggtggc
    23701 cagcgatttg gggagatgga ggtctgggca ctgctcggcc atggcgtggc acatacgctt
    23761 cgggaaatgc tcacgatcaa gtcggatgat atcgttgggc ggtctgcggc atttgattct
    23821 cttgtaaaag gcgagaagat aaatcagtcg aatattccgg cgtcgtttaa cgtcctgtta
    23881 aacaatttgc gggggcttgc gctcgatgtc gaagttaaaa aagatgcgag cgttataaga
    23941 gaaaaataaa gaaaatgaac cacagaacca aaaacataaa tgtgaatgac ttcgactcga
    24001 taacgttgaa actagcatca cctcagcgca ttaaagaatg gtcatatggc gaggtcacaa
    24061 aaccagaaac gatcaactat cgaacccagc gcagtgaaaa aagtggctta ttcgatgaga
    24121 aaattttcgg gcccgaccga gattatgaat gttactgcgg aaaatatcgc ggcatcaggt
    24181 acaagggtat agtttgtgaa aaatgcggcg tagaaataac gagatcaatc gttcgacgtg
    24241 aacgaatggg gcacatcgat ctcgccactc ccgtttctca tatttggttt ttgagaagta
    24301 tgccgtcgcg catcgggctt gtgctcggta tgtccactgc cgacctcgag aaggtaattt
    24361 actttgcagg ctacattatt acgcacgtaa acgaggacga gcgcgaacaa ttacttaagg
    24421 atctcgactc ggaatacaag gctaaggtta aaaatttgca ggaggagaaa tccaaggatg
    24481 ccatcaaaga gcttctgctt aatgccaaaa aagagatcga tatgattcag gaaggtgtcg
    24541 tacttgatga ggttcagtat cacaactatt cggtaaaata cggaacgctt tttgaggctg
    24601 aaatcggtgc tgaagccgta tacaaaattt taaaggagct tgatatggac aagttactgg
    24661 agcggcttgt tcgtgagaaa gataaggcgg gttcaataga acgagagaaa ataaataaac
    24721 gtatttcgct tctgaaatcg atgatcagcg ccgctattcg tccggaatgg atgtttctga
    24781 cgtccattcc cgtaattccg cctgcccttc gaccgatggt cccgctcgat ggcgggcgtt
    24841 atgccacttc ggatgtaaac gatctctatc gccgagttat caacagaaat aatcgcttgc
    24901 gaaaacttaa ggagatcaat gctccggatg taattttgcg aaacgaaaag agaattctgc
    24961 aagaagcggt ggatgcgctt atcgataact cgattcgtca cggcaatagc aacgcagggg
    25021 ctttaaacca aatgcaacgt cgaccgctga aatcgctctc ggacaacttg aaaggaaagc
    25081 gcggactgtt caggcaaaac cttctcggca agcgtgtaga ctattcgggt cgctcggtga
    25141 ttgtagtggg acctgatctt aagctcaatc agtgcggact gccaaagcac atggcgcttg
    25201 agctgtttcg gccattcgtc attgcccagc ttttgaagaa ggaatatgca tataacatcc
    25261 gcggggccaa ccgtttgatt gaagaggact tgcctgaagt ctgggcgatt ctcgaggaag
    25321 taattgcgga caagcacgtt cttttgaatc gtgctccgac gcttcaccgc ctcggtatcc
    25381 aggcattccg gcccgtgctg attgaaggta atgcgatcca gattcatcca cttgtctgta
    25441 ctgcattcaa tgccgacttc gacggtgacc agatggctgt gcacgttccg ctttcggatg
    25501 aagcgcagat tgaggcacgc gagatcatgt cagcggatag aaacatttta aagcccggaa
    25561 acggcgagcc gacagtatcc ggcaagctgc tcgatattat cctaggatgt tattggatga
    25621 caaaggaaac gactggcggc ctcggggagg gaaagttttt tccgtcaccg aacgcggcca
    25681 taactgcatt tgacttcggc gttgttgaat ttcgcgcgaa aataaaggtg cttgccactg
    25741 acaccgtaaa atataagcaa ttcgattcca agatttttga gactactgta ggacgcctgc
    25801 tcttcaattc cgtcttgccg tcagactatc catatatcaa taaagaaatt gcgcgaaaac
    25861 agatggctgc gctaatcgac gacctgatcg acaaatacgg aattgacaat gtttctccga
    25921 tgctcgacaa aatcaagggg ttcggtttta agtacgcgac gcactcggga accacgtggg
    25981 gcattgatga cattcaggag cctattgcca aagatggagt tatcaaggaa gcgcagaaaa
    26041 aagccgccga ggttgatgaa cattacgact tgggttttct ttcaaaggag gagcgccttc
    26101 gaaaaaccat cgagatatgg caagcgtgca aagctgaagt ggaaaaactg attcctggaa
    26161 cgctcgataa aaatagttcg gtgtatgaca tggtgtactc cggtgcacga ggctcattct
    26221 cacagattac gcaaatggcg ggcatgaaag gattgatcgc caacacggca ggagagacga
    26281 ttgaatttcc cattctctct tcatcgaaac gcggtctgac tcctattgaa tacttcatta
    26341 caacgcatgg atctcgaaaa ggtctgactg atacggcgct taatactgct cgcgcaggat
    26401 atttgacacg ccggctattc gacgtggcac aggatgcaat cattaccgaa gaggattgcg
    26461 ggacaaaaga aagcattcta attcgaaagg aaacggcatc cggaatggaa gtatcaatcg
    26521 taaaaaacat caagggtaga gttcttgggg aggacgtcac ggataaaaaa ggcaaagtat
    26581 tgttcaagcg agggcacctc attaccaaag aggacggcga taatatcgag gccgcaggag
    26641 tgactgaagt aaaggctcgt tcaccgctcg tctgcaaaac cgggaacggt atttgtatta
    26701 agtgctacgg aaccgatctc ggaaaaaatg ttctcgtcga gcttggcgag gccgtgggaa
    26761 ccgttgccgc tcaagcaatc ggtgaaccgg gaacgcaact taccatgaga acctttcatg
    26821 cgggcggaac ggcatcggta ggcggagaca ttacgcaagg actcccgaga gtagaagagg
    26881 tattcgagaa gcgaaagcca aaaaacccgg ccattgtatc gaaggtagat ggagttgtga
    26941 ctgcagtcaa ggatctcgga aaggagaaga tcatcgtggt tcttccagag gttgattcaa
    27001 aatccaaaac caaaaagaag gctgaagtgg aatacaccgt gaattacaag cgcatggcct
    27061 tggtaaaagt tggaggcaag gttaaaaaag
//
LOCUS       DATDSU010000111         2735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1293450, whole genome shotgun sequence.
ACCESSION   DATDSU010000111 DATDSU010000000
VERSION     DATDSU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293450"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VI981_03830"
     CDS             <1..187
                     /locus_tag="VI981_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479456.1"
                     /translation="EIRGVAKEVSRFTLNGYPIFINEAGEFREERILSPGVSIIELRA
                     EDKFGHEALVRRTVFMK"
     gene            249..557
                     /locus_tag="VI981_03835"
     CDS             249..557
                     /locus_tag="VI981_03835"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEY4479457.1"
                     /translation="MKFYKFTAVFEPEKGEDEVYNVSVPALLGCFSFGDSREEARYNI
                     REAIELYLETFLEEGRPIPADKNKIRVPRGALKEEITVGVDFQVKSGFPAKALITHGR
                     "
     gene            547..771
                     /locus_tag="VI981_03840"
     CDS             547..771
                     /locus_tag="VI981_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEY4479458.1"
                     /translation="MADKLPVLKTDEIVRALFKLGFRSKRKRGSHLILERSGRVIVVA
                     IHEGRDVPRGTLKRILRQANVSLEEFIKYL"
     gene            774..2588
                     /locus_tag="VI981_03845"
     CDS             774..2588
                     /locus_tag="VI981_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479459.1"
                     /translation="MSSETRTCQNCKNSFAIEPEDFKFYEKIKVPPPTWCPECRLIRR
                     LAWRNEYTFYRRKCSAPGHDEELISTFSPDKPFPVYDPHFWWGDDWDSMNYGREYDFS
                     RPFFEQFRELVWAVPKPSLTGEYFSMVNSDYSNWAGHLKNCYLITDADYVEDSAYGTQ
                     ICRVKDSLDNDFLLDSQRSYFNFDVFKSYLIFFSVGCRDSYELYFCKNCVGCNNCFGC
                     TNLRNKSYHIFNKPYVKEDYQKKLSELNLGSHRSLMALKTKAEEFWKQYPEKEMRGYH
                     NLRVTGDYIYNSKNTSHGFFVTDVEDSKFVALTHAKPTKDCFDYTDWGENAQLVYEGM
                     SVGLGAYNIKFSQLIFKEVRDIEYSYFCGNSSNLFGCAGLLKKQYCIFNRQYTRDDYE
                     KLVLKIKKHMDEMPYVDKKGRVYKYGEFFPPELAPFAHNETIIREFFPVKKEEVLANG
                     WLWKDPEPRNYQITLKPGDIPDHIKDVSDSVLSDIIGCEHEGKCDEQCTTAFRLIPQE
                     FAFLKRMGLPLPRLCPNCRHYQRIRQRNPLKLWHRRCECAGSTSSPQAGTEYTYKNTA
                     DHIHHKKDEPCQNEFETTYAPQRPEIVYCEQCYLREVV"
BASE COUNT          778 a          579 c          656 g          722 t
ORIGIN      
        1 ggaaattcgc ggcgtcgcca aagaggtgag ccgtttcact ctgaacggct atccgatttt
       61 cataaacgaa gccggggagt ttcgtgaaga gcgcattctg tccccgggtg tttccatcat
      121 tgaacttcgc gccgaggaca agttcgggca cgaagcgctc gttaggcgga cggtgtttat
      181 gaagtaaatt ccacctggtg tccggagtgc cgcttgccgg tttttctaaa aattgttaat
      241 atttaaacat gaaattttat aaatttaccg ccgtttttga gcctgaaaaa ggagaagatg
      301 aagtttataa tgtttcggtg ccagcgcttc taggctgttt tagtttcgga gacagtcggg
      361 aagaagcccg ttataatatc cgcgaggcca tcgaacttta tctggaaact tttctggaag
      421 aagggagacc aataccggct gataaaaata aaataagggt gccgcgtggc gccttgaaag
      481 aagaaataac cgttggcgtt gattttcagg taaaaagcgg ctttcccgca aaagctttaa
      541 ttactcatgg cagataagtt gcccgtcttg aaaaccgatg aaattgtccg ggctttgttt
      601 aagttaggtt tccgctcaaa aagaaaaaga ggaagccatt taattttaga gcggagcggt
      661 cgggtcatcg tagtcgcaat tcacgagggt cgcgatgttc ctcgcggcac cttaaagcga
      721 attttaagac aagccaatgt ttcgctggaa gaatttataa aatatctttg actatgtctt
      781 ccgagaccag aacctgccag aactgtaaaa attcgttcgc cattgaaccg gaggatttta
      841 agttttacga aaaaatcaaa gtccctccgc ccacctggtg cccggagtgc cgattgatta
      901 ggcggcttgc ttggagaaat gaatatactt tttaccgcag gaaatgtagt gctccggggc
      961 acgatgagga gcttatttca actttttcac ccgataaacc tttcccggtc tatgaccccc
     1021 acttttggtg gggggatgac tgggactcaa tgaactatgg gcgcgagtat gatttttctc
     1081 gtccattctt tgagcaattt agagagctgg tttgggcagt tccgaaaccg agtctgacgg
     1141 gcgagtattt ttccatggtt aatagtgact attctaactg ggcgggacac ttgaagaatt
     1201 gctatctcat cacggatgcg gactatgtcg aggattcggc atacggcacg cagatttgcc
     1261 gcgtgaaaga ctcgcttgac aatgattttt tattggattc ccagcgctct tatttcaatt
     1321 ttgacgtttt caaaagctat ctcatctttt tctccgtagg atgccgcgat tcttacgagc
     1381 tctatttctg caaaaactgc gtggggtgca ataattgttt cgggtgcaca aatctccgga
     1441 acaagtccta tcatattttt aacaaaccct acgtcaaaga agattatcag aaaaagctct
     1501 cggaattaaa cctcggctct catcgcagtt taatggcgtt aaagaccaaa gccgaggagt
     1561 tttggaaaca ataccccgag aaagaaatgc gcggttatca caatctgcgc gtaacagggg
     1621 attacattta taattctaag aatacctcgc atggcttttt cgtaacggac gttgaagaca
     1681 gcaagttcgt ggccctaacc cacgccaaac ccaccaagga ctgttttgac tacaccgact
     1741 ggggcgagaa cgcccagctg gtgtacgagg ggatgagcgt ggggctcggt gcttataaca
     1801 ttaaattcag tcagctgatt ttcaaagagg tacgcgatat tgaatatagt tatttctgcg
     1861 gcaactcttc gaatttgttt ggttgcgctg gtctgcttaa aaaacaatac tgcattttta
     1921 acagacagta taccagagat gattacgaaa agcttgtttt aaaaataaaa aaacacatgg
     1981 atgagatgcc ctacgttgat aaaaagggga gagtttataa atacggtgaa tttttccctc
     2041 ccgaactggc tccgtttgcc cacaatgaga ctattatccg tgagtttttc ccggtaaaga
     2101 aagaggaagt gcttgcgaac gggtggcttt ggaaagaccc cgagccgagg aattatcaga
     2161 taaccttaaa acccggcgac attcccgacc atataaaaga cgtatccgat tctgttttaa
     2221 gcgatattat tggctgtgag catgagggaa aatgcgatga gcagtgcact acggcattca
     2281 gattgattcc tcaagagttc gcgtttttga aacgaatggg gctccctctg ccgcgactct
     2341 gccccaactg caggcactac caacgcattc gccagcgaaa tcctctcaaa ttatggcaca
     2401 gaagatgcga atgcgcgggt tcgacaagct caccgcaagc aggcactgaa tatacctaca
     2461 aaaacaccgc agaccacatc catcacaaga aagacgagcc ctgtcaaaac gaattcgaga
     2521 ctacctatgc tccccagaga ccggagattg tatattgtga acaatgctat ttaagggagg
     2581 tggtataatg gttgagggta atccgcctat gccaaggctt cggcggatgc tcaatttttg
     2641 gcaaaaatta tcagaataat tttttaaaaa ttggcaaaaa agtctgaaga aaaatcggag
     2701 aaactcgcgg gagtggaatc ggctattaaa gaaat
//
LOCUS       DATDSU010000112         4111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_278270, whole genome shotgun sequence.
ACCESSION   DATDSU010000112 DATDSU010000000
VERSION     DATDSU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278270"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..417)
                     /locus_tag="VI981_03850"
     CDS             complement(205..417)
                     /locus_tag="VI981_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479460.1"
                     /translation="MLQKRAGRYHTRFWFEDFTKGRKFRRDFGTNLAVARRKKRLTKK
                     QCTELEFFFRVAIEQIDKSRTESLNA"
     gene            complement(465..881)
                     /locus_tag="VI981_03855"
     CDS             complement(465..881)
                     /locus_tag="VI981_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479461.1"
                     /translation="MKRQSRKKAKAGDLAYLFFKATRFDNPFPAFYTRDSSFETNEVS
                     PARNTLRKHKGSFMEVFTAVGATVINGFYIILRPQMVSGSTRGKRIGVRLSEQHMYLD
                     GTRHIRGVNRVTKQVLYGCLSMKNGCGKFWYVPDVG"
     gene            1221..2225
                     /locus_tag="VI981_03860"
     CDS             1221..2225
                     /locus_tag="VI981_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY4479462.1"
                     /translation="MNELIKKLDKKYGPNRVFWGLIVIFVIVVLIGLVVVDATPSSQW
                     RRSTSGGVAPYGDTAMGKIAYPSIGTVSQEMSQGMMNNIYPVPPPIFYGGYASSKIMP
                     SGITVIRNGSLDLVVENSESALGEIRSITQTNGGFIEYSVLREVSDGVKSGHVTIRIP
                     QDNFDTVFSALKLLARRVNHEVSDVQDMSALSVDLEAQIVNLRAEEEQYRKLLDGSKK
                     IEDTLLITARLSDVRIRIDMLEGQLNNLARESDMASISISLTSEADVEVFGIIWKPLT
                     VLKHALKTMLADLITIVNWLIFFVFRLPGLVITVALLVLFFVVIYKVALWLKVVLTRL
                     "
     gene            2257..2820
                     /locus_tag="VI981_03865"
     CDS             2257..2820
                     /locus_tag="VI981_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY4479463.1"
                     /translation="MEIIYIVIGVIVLVLLWVAFAYNRFVSLINRAKEAWSDIDVQLK
                     RRYDLIPNLVSTVKGYATHESSAFEKVTTARAAAMGAGASPAAKGQAENMLTGALKSL
                     FAVAEAYPDLKANANFLELQHELSDTENKIQAARRFYNGNVRDLNTAVESFPGNMIAG
                     AFHFGKMEFFELEEGEEAAKQPVAVKF"
     gene            2879..3352
                     /locus_tag="VI981_03870"
     CDS             2879..3352
                     /locus_tag="VI981_03870"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4479464.1"
                     /translation="MTPNNQELHRVVATVFIYKDGKYLLVKRSSHTKIFPDKWHVPGG
                     GLATSDYINAKPDTSAGQWYYALTKGLFREVKEEVNLEIEEPKYLLDLAFIQPDGTPV
                     LCLVYYARYKSGEVKLDADGVEFVWVSAVKAKRYDLIPGIEHEIEMVDEILRETN"
     gene            3455..4009
                     /locus_tag="VI981_03875"
     CDS             3455..4009
                     /locus_tag="VI981_03875"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479465.1"
                     /translation="MRTRLGKNQQKLITLLYLGVAFSLCRSPRRQFKIITVDLPKEFA
                     KINMQTLEYGLHRLVDLKYIYVRKIQDGHYQARLTADGCRHVQSINIRNLKIAKSDKW
                     DKKWRMVFFDIPEKMNKKRNLFRENIKAIGFLEVQRSVFCYPYACGTEIIKIANFYGL
                     SDYIHYAVVEELSDAGHYRKKFGL"
BASE COUNT         1156 a          837 c          956 g         1162 t
ORIGIN      
        1 ttgattaggg ttatagtaaa gtttttgaat gatccaaaat gcggtatctt gttcatagtt
       61 catgaccata ccgaaaaatt ttttacttgc aatgggagag ataagtatca tacttggaga
      121 cttagtctcc aagggggggt atgttagcaa ggccttgcta acattagctc ccagtttctt
      181 gtttgccgat ataaaaaaac cgaatcaggc attgagtgat tcggttctgg atttgtcgat
      241 ttgctcgatg gctactcgaa agaaaaattc gagttcagta cactgctttt ttgtaagccg
      301 cttttttctg cgtgcgacag cgagattagt gccaaaatct cttcggaact ttcgcccttt
      361 ggtgaaatct tcaaaccaga agcgcgtgtg ataccttccg gctctctttt gcagcatgcg
      421 ttctggtcca acacatgact caatgagttt ggctacgaag atttttaccc cacgtcgggg
      481 acataccaga actttccgca cccgttcttc attgagaggc atccgtatag cacttgcttt
      541 gtcacgcggt ttacgccccg tatgtggcgg gtgccgtcca gatacatgtg ttgttcggaa
      601 agccgaacgc cgatgcgttt cccgcgcgtg gagccggaaa ccatttgggg acgcaagatg
      661 atatagaagc cgttgatgac cgtcgcgccg acggcagtga atacttccat aaaagaaccc
      721 ttgtgtttgc gaagcgtgtt ccttgcgggc gacacttcat ttgtttcaaa gctactatcg
      781 cgagtgtaga aggcgggaaa gggattgtca aatcttgtag ccttgaaaaa caaataggct
      841 aaatctccgg ccttggcctt ttttctcgat tgtcttttca taatctctat tgggttcaga
      901 ttcagaggag taaaccccgt tagaagctca tacgggcatt aaaccccgtg ttcgctaaaa
      961 tcaaattgca ttcatccacg cggcaagccc cgttagaaac tttcaatata tggaacaagg
     1021 aaaatatcgt atgtctcgct tccgcatggc ttggtttcta acggggcagg ccgcggggcg
     1081 ttctgcttct aacggggtaa acaagaagta gccgtttaat gtcacttgtg gtccaagtta
     1141 tccacggcac tttttttaaa tatgcagtaa aatataaaca ttagtggcgc agaaatgtgc
     1201 caagctctta atgtctaaca atgaatgaac ttataaaaaa actcgataaa aagtacggcc
     1261 cgaaccgagt gttctggggc ctcatagtca tttttgtcat agtagtactg attggtctgg
     1321 ttgttgttga cgccactccg agttctcaat ggagacgaag cacgagcgga ggcgtcgcgc
     1381 cgtatggcga cacggctatg ggtaaaatag cttatccatc aatcggtact gtgtctcaag
     1441 aaatgtctca aggaatgatg aataacatat atccggttcc acctccgatt ttttatggcg
     1501 gctatgcatc ctcaaaaata atgccgtctg gaattacggt catccgaaac ggttcgcttg
     1561 atctcgttgt cgaaaattct gaaagcgccc tcggcgagat acgaagcatt acccagacga
     1621 acggcggttt tatcgaatat tctgttttgc gtgaagtttc tgacggtgtg aagtccggcc
     1681 atgttacgat tcgaattccg caggataatt ttgacacggt attttctgca ctcaaattac
     1741 tcgcgcgaag agtcaatcac gaggtttctg atgtgcaaga tatgagtgcg cttagcgttg
     1801 acctcgaagc tcaaatagtc aacttacggg ctgaagaaga acaatatagg aaacttctcg
     1861 atggatcgaa gaaaattgaa gatacacttc tgattacggc aaggcttagc gatgttcgaa
     1921 taagaatcga catgctcgaa gggcaactca ataatctggc gcgcgagagt gatatggcat
     1981 caatctcgat ttcgcttacc tctgaagcgg acgtagaagt ttttggaatt atctggaaac
     2041 cgctcaccgt attgaagcat gcactgaaga caatgctcgc tgatctcatt acgattgtaa
     2101 actggcttat cttcttcgta ttccgtctgc cgggtcttgt aatcacagtc gcgctccttg
     2161 tcctcttctt tgtggttatt tataaggtag ctctctggct taaggttgtc ttgacgaggt
     2221 tatagacgta taatcttgtt ttgatatact tcatttatgg aaattatcta catagtgata
     2281 ggtgtcatag tgcttgtgct tttgtgggta gcttttgcct acaatcgttt tgtctctctt
     2341 attaatcgcg caaaagaagc gtggtcagat atagatgttc aattaaagcg ccgatatgat
     2401 ctcattccga atctcgtaag cacggttaag ggttacgcaa cccacgaaag ttctgcattt
     2461 gagaaagtga cgacagcacg cgcggcggcc atgggtgcgg gcgcaagtcc tgcggcaaaa
     2521 ggccaagctg aaaatatgct aacgggagcg cttaagtcgc tttttgcggt ggccgaggcg
     2581 taccctgatt taaaagcaaa cgcaaacttt ctggaacttc aacatgagct ttctgacaca
     2641 gaaaataaaa ttcaagccgc tcggcgcttt tacaacggta atgtgcgcga tttaaacacc
     2701 gcagtcgagt cttttccggg aaatatgatc gcgggcgcat tccactttgg taaaatggaa
     2761 ttcttcgagc ttgaagaagg agaagaagcc gccaaacagc cggtcgcagt gaaattctag
     2821 ttgtaagtct gcttggcgcc aagtttcaat cttacagctc ggaaaaaacc ggaaacgtat
     2881 gactcccaac aatcaagaac tccacagagt tgttgcaact gtgtttatct ataaggatgg
     2941 aaaatatctt ttagtaaagc ggtcttctca cactaaaatt tttcccgata agtggcatgt
     3001 tcccggaggg ggattggcga caagcgatta tataaatgcc aaacccgaca catctgccgg
     3061 acaatggtat tacgcgctca caaaaggcct tttccgcgag gtgaaagagg aagtaaatct
     3121 tgaaattgaa gagccgaagt atttactcga tcttgctttc attcagcctg atggaactcc
     3181 agttctttgc cttgtttatt atgctcgata caaaagcgga gaagtaaaac tcgatgctga
     3241 cggtgtggag tttgtctggg tgagtgcagt caaggcaaaa aggtatgacc ttattccggg
     3301 cattgagcac gaaatagaaa tggttgatga gattttgcgc gagacgaact aacaattatg
     3361 ccacctatat gctatcatca ttatttatct acgatcgtag ataaataata attctaagca
     3421 tctagtaaga taccagatgg ggatttgaaa atacatgcgt acacgtttag gaaaaaacca
     3481 acagaaatta attactcttt tatatcttgg ggtagcattc agtctctgca ggtctcctag
     3541 aaggcaattt aaaattatta ccgtagatct gcctaaagaa tttgcaaaaa taaatatgca
     3601 gactcttgag tacggtttgc accgattagt agatttaaaa tacatatacg taaggaaaat
     3661 acaggatggt cactatcagg ctcggctcac agccgatggt tgcagacacg ttcaatcgat
     3721 taatatacga aatctaaaaa ttgccaaatc cgataaatgg gataagaagt ggagaatggt
     3781 gttttttgat attcctgaaa aaatgaataa gaagcgtaat ttgtttaggg aaaatataaa
     3841 ggcaatcggt tttcttgagg ttcaacggtc tgtattctgt tacccatacg catgcggtac
     3901 tgaaattatc aagatagcaa atttttatgg gcttagtgat tatatacact atgctgtagt
     3961 ggaggagctg agcgatgcgg gtcattatcg gaaaaaattt ggtctataaa atcagattta
     4021 tctacgatcg tagataaata ataatagaaa tataggcccc tgcggcctgc cgcagggtaa
     4081 tctaggaacc cgcttcgctc gtctaccgtc c
//
LOCUS       DATDSU010000113        22771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2278747, whole genome shotgun sequence.
ACCESSION   DATDSU010000113 DATDSU010000000
VERSION     DATDSU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 22771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22771
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278747"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /gene="thyA"
                     /locus_tag="VI981_03880"
     CDS             <1..560
                     /gene="thyA"
                     /locus_tag="VI981_03880"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY4479466.1"
                     /translation="DLGPGSYGAAFHDFPTSEGQPFNQWKHIVEQIIEEPQLRTHFVS
                     PWIPQYMGRGKGKQQKVVVCPCHGWVHVRIIDGKLSLHMFQRSCDVPLGLPQNMVQYS
                     ALTLALAQVTGYEPYEYIHSFSDAHIYVNQIPAVETMLAREPRKLATMKVDPNIKDMF
                     AFRKEHFTLEDYNPHPGIKGIPVAV"
     gene            667..1164
                     /locus_tag="VI981_03885"
     CDS             667..1164
                     /locus_tag="VI981_03885"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY4479467.1"
                     /translation="MISIIAAVSKNGVIGKDNDLPWNLPNDRKYFADTTRGKPVIMGR
                     KTFESIVARIGKPLPGRRNIIITRNTDYKPSGTEIVHSLKDAVSLVKAESEAFIIGGA
                     EIYKDAIPFADRIYLTQVETEIEGDAYFPDVTIDKWDLKRAVSNKKDAKNEHDYTFLI
                     YERKR"
     gene            1148..1660
                     /locus_tag="VI981_03890"
     CDS             1148..1660
                     /locus_tag="VI981_03890"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY4479468.1"
                     /translation="MKEKDKKSKKKTIEENRYINMRTIRRVDQRKVMEKIKKAGHCPF
                     CRENLDKYHKNPILKEGKYWLLTDNQWPYEKIKHQMLAIYKTHVEHLKDIDPEAGKEL
                     FEMFGQISKERGMSGGGVAMRFGSSLHGNYGSSVLHIHAHLIEPDLEKLNSDEAWRFK
                     FGQPKNYKKS"
     gene            1732..2307
                     /locus_tag="VI981_03895"
     CDS             1732..2307
                     /locus_tag="VI981_03895"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY4479469.1"
                     /translation="MPELIIPEITDASTLACISRSRKFTQYARAVDNALKGHCPFCKI
                     DKAYNKVEIENDLCLAWHSNPPEDNTALHFLIVPRRHVKDSMELTDVELLTMWKTIPR
                     ELVKRFAIKSRGLLMRDGDATLSAGTIEHLHLHLMVPNGTGRVESPFYKGAAEESKSL
                     SRAIVFEKLRTGVAFEALGDAERELVRDRLI"
     gene            complement(2968..3804)
                     /locus_tag="VI981_03900"
     CDS             complement(2968..3804)
                     /locus_tag="VI981_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEY4479470.1"
                     /translation="MNKVIVAYVPVIHEGYRRFFESHATEARDLYIFGPEIIKHYRQI
                     DKDINALPPEVTKKFIEALGFFKHVHILDEKNILDINGEALIMPDEDVSRTLAQKYLS
                     KSGITWDSVFLRWDKHKTMEAKPVEADQKISREAFDKSVVSKLKKESEKSSDFWRRIG
                     AAVVKNGEIILTAYNEHLPSAHSPHIHGDPRNNFHKGVALELSTAIHSEARLVAEAAR
                     KGIPLEGVSLYVTTFPCPPCAKQIAFSGIKKLFYTGGYVMLQQDKILKSRGVEIIFVE
                     GN"
     gene            complement(3864..4478)
                     /locus_tag="VI981_03905"
     CDS             complement(3864..4478)
                     /locus_tag="VI981_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY4479471.1"
                     /translation="MKELNLNTHIGIGIFGAGSNFEERWIRDDRKLSKFVENCKGLGL
                     KIVLTMGTYDMVHIGHARYFEAAKKHGDILIIGVDSDEKVRARKGPDRPVVPQAERLE
                     MVTHLRSVDVVYLKKHKAPRWHLIKLIRPDTLVATKRTYSRLDLKNLKKFCKEIVVLE
                     PKATTTTSAKIRLLQISTAKKLERTLTPKLLDVIKESLSAVDKK"
     gene            complement(4536..4820)
                     /locus_tag="VI981_03910"
     CDS             complement(4536..4820)
                     /locus_tag="VI981_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479472.1"
                     /translation="MEEKLEKNRKRVGVTLQIMGAIVVSIDLFGLVLLFLLSRPTSFS
                     LFVESNARLLYEIVAAVLVIYSGYLYERAKVRHLYFVFLSVCLLAASFIV"
     gene            complement(5413..5835)
                     /gene="rplK"
                     /locus_tag="VI981_03915"
     CDS             complement(5413..5835)
                     /gene="rplK"
                     /locus_tag="VI981_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY4479473.1"
                     /translation="MAKKITKKLKLVIPAGKANPAPPVGPALGQAKINIGEFVTRFNE
                     ATKGMMGDIVPVEVLVYEDRTFDMVLKTPPASALILKAIGAEKGSGKNKVQKAGTISR
                     SKIKEIAEKKMKDLNANDINAAMKIIEGSARSMGVEVK"
     gene            complement(6000..6545)
                     /gene="nusG"
                     /locus_tag="VI981_03920"
     CDS             complement(6000..6545)
                     /gene="nusG"
                     /locus_tag="VI981_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY4479474.1"
                     /translation="MSKQKLKEERNWYAIHTYAGYENAVARNLKQRIESLGMENKIFN
                     VLVPTEKKIKIKAGKRVEEEEKIYPGYILVDMIVTDDSWYVVRNTPRVTGFVGSGVYP
                     VPLERKEVDILFKRMNSETVKHNIDVQPDDPIIIVDGPFKDLEGKVSEVDEERGKVKV
                     LVSMFGRETPVELDFLQVKKI"
     gene            complement(6738..6914)
                     /gene="secE"
                     /locus_tag="VI981_03925"
     CDS             complement(6738..6914)
                     /gene="secE"
                     /locus_tag="VI981_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY4479475.1"
                     /translation="MKIIDYFRETKAEMKHVSWPTRQQAIFYTVIVIAISLVTGAILG
                     LFDYLASFVIEFLI"
     gene            complement(7301..8092)
                     /locus_tag="VI981_03930"
     CDS             complement(7301..8092)
                     /locus_tag="VI981_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY4479476.1"
                     /translation="MDENKTKMWQALRFALFIVAIFLVVKTIGEIKGIGYVGKNPTQQ
                     NTISVSGEGEVFVKPDIAQLTFDVFSEKSTVSAAQEDVTKRMNEIIAYLKAEGIEEKD
                     IKTINYQESPKYDYQARYQPCLPNGLCPPQGVPKLVGYTVTHSIQLKIRKIDDSGKIL
                     EGLGSRKVSNMGSLYFTVEDEKVPQNESRKMAIDDAREKAQRLARDLGVKLVRVVSFN
                     ESGNYPIYFKAEAMDRGADVSSAPPEIPTGENKISSNVTILYEIR"
     gene            complement(8410..9291)
                     /locus_tag="VI981_03935"
     CDS             complement(8410..9291)
                     /locus_tag="VI981_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479477.1"
                     /translation="MKKISKTIITLGLLGSFLFSASTANAALTQSQIDAVIGLLQAFG
                     AEAKVINDVRASLGGQAPAPQSATFCYNWTRNLRVGDKGADVDALGKVLQKEGLNIPG
                     YAYSGIDDSLTYNVFVVTAVTGFQEKYASEILKPNLLSRGTGYVGAATRAKLNALYGC
                     SKPATPTACSNGATNYPRCNANLLFNPSITVLSPNGGETFRSDDTISISFKTILQEGP
                     FNLYLKNGREDMAYMSIDSYISGSNIVAFRPSARLLAGGTLVLGTNYRITVCNRNADV
                     TKIICDSSDAPFTISAN"
     gene            complement(9482..10036)
                     /locus_tag="VI981_03940"
     CDS             complement(9482..10036)
                     /locus_tag="VI981_03940"
                     /inference="COORDINATES: protein motif:HMM:NF044910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479478.1"
                     /translation="MGVIEKRVRKTRIKKILLETVGAVGGLALAMAAPNMLKLFRYKT
                     SNSSAYQSIRRLAESGFIVISGEQGMRTCALTKKGDLYIKRIRELEKFSIPRRRWDGK
                     WRILSFDISEKNRNNRNLLRGTLSKVGFVRLHHSAWVYPHNCEDFIQLLKLDFKFGRN
                     VLYIVAEEIENDYWLRKHFGLPLR"
     gene            complement(10166..11035)
                     /locus_tag="VI981_03945"
     CDS             complement(10166..11035)
                     /locus_tag="VI981_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY4479479.1"
                     /translation="MAKLLDGKMARQARALSLKAEIEKMGAKPTLAIIQVGDRAESSA
                     YIEAKKKFGKEIGAIVIHIQCPTESKTSEIQEKIDELNENPKINGIILQLPVPPSIDA
                     YLLVEKIDPSKDVDGQTGESMRRLLRAERGFIPATPKGIFMLLDFYGIPVEGKHIVII
                     GRSYLVGKPAGLLGLNRNATVTICHSKTSNLQEISKSADIVIAAAGQPGLIDKNFVRT
                     GQVIIDVGINEKKGGHLEEIAGRRLVGDVNFADVEPIVEAISPVPGGVGPMTVCALFE
                     NLVLAATLQQNSK"
     gene            complement(11339..12475)
                     /locus_tag="VI981_03950"
     CDS             complement(11339..12475)
                     /locus_tag="VI981_03950"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEY4479480.1"
                     /translation="MDPVPGKKNISLSASEKDRIKESILFFMQTHPLKGKGKESIFIW
                     AGFSLFFTRYSAATLTLIVLILFGTGSALASNALPNEFLYPLKRGVNERVAGWIAIGA
                     KAEAELHADLAMRRLEEAEKLAVQGELDAETSARIRGDFVAHSESARVNVARLRSNNG
                     HEDALDIAGKMESGFAVHDTVLAQIKTDNSVLDGISSSVNAQVSSAAEARIDAESTVR
                     SHAKFNSAPIAEAARKKAEKRIKKTRDFAKSKKVNGAVTIELDHASENYAEGEAYVSV
                     NAYNNAVVAFKNGEKKAEGARLAVRVKDELGIDVMREVNASIAADTALMLNATSSSGV
                     STTTDSSDEETKEEEADRPIKADKRDKKGDEPEPKPPIDIDIGL"
     gene            complement(12476..12988)
                     /locus_tag="VI981_03955"
     CDS             complement(12476..12988)
                     /locus_tag="VI981_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4479481.1"
                     /translation="MRTPNEDISENFIKTYDELADPLYRRFCFRLSNRERSKELVQET
                     FTRSWEYLVEGKEIKNLKSFIYKIANNLIIDEYRKKKSVSLDVMRDDGFDVSTEVDGH
                     ESIVKQAETEHMRKAIDQLPPKYREIVVMRYIDGLTLGEISAILGETENTVSVRINRS
                     IKKIQDLLKI"
     gene            complement(13212..13916)
                     /locus_tag="VI981_03960"
     CDS             complement(13212..13916)
                     /locus_tag="VI981_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY4479482.1"
                     /translation="MTYILILLLIALAGFYGYMRKSNLFDARQKNLFLIGFAFYALFC
                     GVVLFLAFPFVARLNDAIDLLFWNVQSDTADAFFKIIAFLFSTKVIGIFVLLAAAHAY
                     FSARKTEAFSIAVSLVAAASVGEILKSFFQIERPLGPLVDTSGFSFPSGHAILTAVLF
                     FSLTNIIGRIPRRGTRIFLNSAIFVLCLAVLISRMYLHAHWFTDIAGGFFLGTSIALL
                     FNCASQVFKLKSTRTW"
     gene            complement(13928..14377)
                     /gene="mscL"
                     /locus_tag="VI981_03965"
     CDS             complement(13928..14377)
                     /gene="mscL"
                     /locus_tag="VI981_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY4479483.1"
                     /translation="MRNVLREFKEFAVRGSVIDLAVGVIIGAAFNKIVNSLVNDILLP
                     PIGLLLRRVDFSNLFVNLGGERFGTIADAQKVGAPTLNYGLFINALISFLLTALAVFM
                     VVRAINRFRRREEAKVAPELMACPFCRLDIPKKATRCPHCTSTVSSS"
     gene            complement(14379..15017)
                     /locus_tag="VI981_03970"
     CDS             complement(14379..15017)
                     /locus_tag="VI981_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4479484.1"
                     /translation="MIITKIGHCCLVIEENGLRILTDPGSYSTGQSDVRDLDVILITH
                     EHADHLHIPSLRAILKNNPKALVLTNRGVAAILDAEKISYTVLEDGESTVEREVTIAG
                     HGKKHALIYPGWKEVVNTGYFIANKFFYPGDAFLDPKIPVEVLALPVAGPWMKISEAI
                     DYALIIKPKIAFPVHDGMLKPIAKTHHLVPGKILAEHEIEFRAMNAGDRTSF"
     gene            complement(15004..15432)
                     /locus_tag="VI981_03975"
     CDS             complement(15004..15432)
                     /locus_tag="VI981_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479485.1"
                     /translation="MKTKTKFITLTVVMAVATFLLTHVIWPNDPSMQSPSSTLIPFFI
                     ILDIIESIAFGVGVSFVVWGRVYIWRFGIWRQPAGWAFGYIVWALVSWWPHDNFHRVI
                     GMNLKHLLYVEYIFHVTLILGAMLAAYWFYFASHIFHDHH"
     gene            complement(15437..15694)
                     /locus_tag="VI981_03980"
     CDS             complement(15437..15694)
                     /locus_tag="VI981_03980"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY4479486.1"
                     /translation="METGSFKEKVFSIVSKIPRGSVLTYKEVALLCGRPLAYREVGNI
                     LNTNYKRSIPCHRVVRSDGSAGGYNRGTKNKIILLEKERAY"
     gene            15967..16755
                     /locus_tag="VI981_03985"
     CDS             15967..16755
                     /locus_tag="VI981_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY4479487.1"
                     /translation="MKTIILAAGYATRLYPLTLTKPKPLLQVAHKPMLERVLEKLALA
                     KDDEVIIVTNEKFRGNFERFADSYKRIHGISLKVLNDGSSSDENKLGAIGDLHLAIEK
                     ENIAEDILVIAGDNLFSESLAPFREYAVRKNAPVLAVYDIGSIEATKKFNAISVNEES
                     VITFFEEKPSRPQSSLMGIALYYYPKNIVPLIKTYIQEGNNADQPGRFIQWLYKKMPV
                     YTWRVPGIWFDIGSKETLDEANQAFAEIALEKKGAHVPELEYEV"
     gene            complement(16766..18064)
                     /locus_tag="VI981_03990"
     CDS             complement(16766..18064)
                     /locus_tag="VI981_03990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY4479488.1"
                     /translation="MSWNHFSHAILHTDGDSFFASCEVAQNPALRGKPVVTGKERGIA
                     SAMTYEAKARGVKRGMSLFEIRRVCPEAIILPSDYETYSLFSKRMFEIVRRYTPIVEE
                     YSIDECFAQLSGLRRTFRCSYGEIAAKIKHDLQTELGLTFSMGVAPTKVLAKVASKWK
                     KPDGLTVISQNEIESYLCNLPCAKIWGIGENTAAFLEKKGVHTALQFIEKPNWWIMEH
                     LSKPFQEIWLELRGEMVYKLNTAEKHEYQSISKTRTFTPPSKDRAFVFSQLSKNIENA
                     CIKARRHKLVAAKFSFYLKTQEFRYHGFDLKLSHTSALPEELVPLARRYFDEVWQEKT
                     LYRATGAVLFELQGAEISQPDLFGGCIKAEAVSLVHQKIDEIALRHGKHTVFLASSLA
                     AMNTPQHDGDRGFKALRKRNLFYGESARRRLNIPLLGEVT"
     gene            complement(18224..18811)
                     /locus_tag="VI981_03995"
     CDS             complement(18224..18811)
                     /locus_tag="VI981_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479489.1"
                     /translation="MKTTTRSRQFARGPASESNPQPAVAPPPPQSSPQTPPPAPEKTP
                     TRQQCFRDELAAIYAGRITPEAEIYSPLVEADILKDQLVGVLSSQRAKEFFSLGTRLR
                     NEALLRPAPRVPGDVEKWSHERAEIEALSELAAKLFWMELRAEFPEAEKKGDVLGVTS
                     DWRVTVSSHSSREDSSGLPLGLLASLLRHGFPGVE"
     gene            complement(18950..19207)
                     /locus_tag="VI981_04000"
     CDS             complement(18950..19207)
                     /locus_tag="VI981_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HEY4479490.1"
                     /translation="MIILFNKNFVKKQKKLSAKVVARLLGRLELFESDFQNPTLNNHS
                     LQGKLNGYWSINITGDLRAVYYFKTTESIVFTDIDTHSNLY"
     gene            complement(19204..19488)
                     /locus_tag="VI981_04005"
     CDS             complement(19204..19488)
                     /locus_tag="VI981_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479491.1"
                     /translation="MKTSIINIKTDPKLKREAQKVARELGVPLSVPINAYLQQFIATK
                     EVHLYTPQKLSARYEKKLKKWEDDIKRGRNLSPAFTNMKDAIKYLKKQRA"
     gene            complement(19503..19781)
                     /locus_tag="VI981_04010"
     CDS             complement(19503..19781)
                     /locus_tag="VI981_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479492.1"
                     /translation="MFYTYILQSERNDEIYIGFAVDLKRRLIEHNQGLNPSTKRYMPW
                     ILIYYEACMNKKDALRRERYFKTTQGRRLLKRRIKEFFYEQKSKDLTN"
     gene            complement(19862..20017)
                     /locus_tag="VI981_04015"
     CDS             complement(19862..20017)
                     /locus_tag="VI981_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479493.1"
                     /translation="MIKDSENDKKDLSIDNRLNDVTKMNAYSGSFDFLKEEPDLYKDY
                     NFRKASE"
     gene            complement(20096..20659)
                     /gene="lexA"
                     /locus_tag="VI981_04020"
     CDS             complement(20096..20659)
                     /gene="lexA"
                     /locus_tag="VI981_04020"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY4479494.1"
                     /translation="MESYKKKITHFYKTHRRLPSYAEIAVLLGFRSKNAAYKLAQKLI
                     DLKFISKDKEGKLIPQNIFGDLRLLGLVEAGFPSPAEEELADTMSLDEYLIPNKEASY
                     MLKVKGDSMKNAGICEGDMVIVERTNSPKAGDIVIAEVDGAWTMKYLRKRGTHIYLEA
                     ANEKYKPLVPKNDLKIAAVVRAVVRKY"
     gene            21359..21733
                     /locus_tag="VI981_04025"
     CDS             21359..21733
                     /locus_tag="VI981_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479495.1"
                     /translation="MMPNSEKFARFVLFTIYIWFGLLKIWGTSPANPLVEALLSRTLP
                     FVSFPTFMLFLGTFEVIIGLLFLIPLWTRATKIIFAIHMAVVFLPLIFLPAIAWQGTF
                     VPTLEGQYILKNLALIALVAGL"
     gene            complement(21773..22255)
                     /locus_tag="VI981_04030"
     CDS             complement(21773..22255)
                     /locus_tag="VI981_04030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4479496.1"
                     /translation="MPAKKISLENAKKDKLFYFVANVIVFRETDGRCLLLKRDLREKV
                     HPSKWGVIGGKLGWSDLPLDAPSRRNGDVLDFENTVEDLLVREAKEEAGIEIEPKLTY
                     INSVAFVRPDGIPVAMVKFDAPYKGGEVLLERGAFTDFKWVDAEEAKRLDCIEEFLWK
                     "
     gene            complement(22255..22599)
                     /locus_tag="VI981_04035"
     CDS             complement(22255..22599)
                     /locus_tag="VI981_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479497.1"
                     /translation="MGDTSRMSGELKKQIDNERLKVSSFYNEIRNKKSELERKKTELT
                     SREREVDALKQKISELERKNAEVIGSRQEINTLTEKIKELEREAAKVESAERTHQHEL
                     YQIEQEANKLKK"
BASE COUNT         6356 a         5223 c         4573 g         6619 t
ORIGIN      
        1 gagatctggg acccggatcg tacggcgcgg catttcacga ctttcccacg tcggaaggac
       61 aacccttcaa ccaatggaaa catattgtcg agcaaatcat tgaagagcct caattgcgca
      121 cgcattttgt gtcaccgtgg attcctcaat atatgggccg cggaaaaggc aaacaacaaa
      181 aagtggtggt ctgcccgtgc catggatggg tacatgttcg aataattgac ggaaagctta
      241 gtctgcacat gttccaaaga tcgtgcgatg tccctcttgg cctgccgcaa aacatggtgc
      301 aatattcggc tcttactctg gcgctcgccc aagtcaccgg atatgagcca tatgaataca
      361 ttcacagctt ttcggatgcc catatttatg taaaccaaat accggcggtt gaaacaatgc
      421 ttgctcgaga accacggaaa ctggcgacca tgaaagtgga tccgaatata aaagatatgt
      481 tcgccttcag aaaggagcat tttacgctgg aagactacaa tccgcatccg ggaataaaag
      541 gtatccccgt agcggtctaa taattttcaa ttctcaatta tcaatgatca atgacgtctc
      601 aatcaataaa ttttcaaata acaaaatatt gaaaatggca gaactgaaac ttgataatct
      661 actttaatga tttcaattat cgcggcagtc tcaaaaaatg gcgtcattgg aaaagacaac
      721 gatctgcctt ggaatttgcc aaacgatcga aaatactttg ccgatacgac acgaggaaag
      781 cccgtcatca tgggcaggaa aacttttgaa tccatcgtgg cacgaatcgg caaaccactt
      841 ccaggccgac gaaatattat catcacacgg aatacagact ataagccgtc tgggactgaa
      901 attgtccatt cacttaagga cgcggtaagt ctcgttaaag cagagtctga agcgttcatc
      961 atcggcggcg ccgagatata caaagatgcc attccttttg ccgatagaat atatctcacg
     1021 caagttgaaa cagaaattga gggtgatgca tactttcccg acgtaactat agataaatgg
     1081 gatttaaaac gagcagtgtc gaacaaaaaa gatgcgaaaa acgagcacga ctacactttt
     1141 ttaatatatg aaagaaaaag ataaaaaaag taaaaagaaa acaattgagg aaaatcgata
     1201 catcaatatg cgcacgatcc gacgcgtcga ccagcgaaag gtcatggaga aaataaaaaa
     1261 agccggtcac tgcccttttt gcagagaaaa tttagacaaa tatcataaaa atccaatcct
     1321 aaaagaagga aagtattggc tcctcactga caatcaatgg ccctatgaaa aaataaaaca
     1381 tcagatgctc gctatttata agactcatgt tgagcactta aaagatattg atccagaagc
     1441 gggaaaggaa cttttcgaaa tgttcggcca gatctcaaaa gagaggggta tgagtggagg
     1501 cggagttgca atgcgcttcg gttcatctct ccacggaaac tacggctcaa gtgtgcttca
     1561 cattcatgcg cacttgattg aaccagatct ggagaaatta aattctgacg aggcatggcg
     1621 ttttaaattc ggccagccca aaaactacaa aaaaagttga caacatctgt ctggaagcta
     1681 tagtgaagtt cgctacagaa ccatcaacac caaaatgaaa gcgagatact catgcccgaa
     1741 ctcatcattc cagagatcac tgacgcaagc acgcttgcat gcatttcaag atcaagaaag
     1801 ttcactcaat acgcgcgagc cgtcgataat gcactaaagg gacactgccc tttttgcaaa
     1861 atcgacaagg cctacaacaa agttgaaatc gaaaatgacc tgtgccttgc gtggcattcc
     1921 aatccgccag aggataacac cgctctccat ttcctcatcg tgccgcggcg gcacgtcaag
     1981 gacagtatgg agcttaccga cgtggagctc ctcacgatgt ggaagacaat tccaagagag
     2041 cttgttaagc gatttgcgat caaaagccgc gggcttctta tgcgcgacgg cgatgccacg
     2101 ctttctgcag gaactatcga acatctccac cttcacctca tggtgccaaa tggcacagga
     2161 cgagtggaat cgcctttcta caaaggcgca gcagaagaat ctaaaagtct ctcccgcgcg
     2221 atcgtttttg agaaactgcg cacaggagtc gcttttgaag cactcggcga cgccgagcga
     2281 gaactcgtca gagatcgcct catctaatcg cttcacacgt ggttcatata atcaaaacag
     2341 taccctcatg gtactgtttt ttctatgagc ggccatcggc gcgcggctag accacgggtc
     2401 tcgaaaaaaa catatgctgt tgaaagaaag cttgtgcatc ttatatgggc tgtgtggaca
     2461 cgaggcacgc cgtttgagaa tcggtaacag aacttaaggt ggcaaacata accggcagac
     2521 gggtgtcggc tattccacat gtttgctgtt tgacttttat atacctagtt tttttattca
     2581 tctaaactca tccagatttt ttaccaaagg cgccaccaca tccctttctc ttcgaaattg
     2641 tgcgacgctt ccccaccctc tatccatacg ttatgtacgt attctccaca tacattactt
     2701 ctacgcacgt gcgtaaaatt aatgtatgtg gcattttacg gaaatttaat caaaacgcca
     2761 ataaattttc acatcatgat gcaataattt tgacaagacg aaattcgggt tcattattca
     2821 caaaatatac acagcttata aacaggttta tccactgttt tcgatatctg cataaataat
     2881 ttcaacttct ttgcttttta tttttcacac ctacattaaa attacgcggc cgcgtaattt
     2941 taatgtatgg ctgtttggaa tcgtagttta atttccttca acaaaaataa tttcgacgcc
     3001 tctgcttttt aatattttat cctgctggag cataacgtag cctcctgtgt aaaaaagttt
     3061 tttgatgcca gaaaaggcta tttgctttgc gcatggaggg cacggaaatg ttgtgacgta
     3121 aagcgaaaca ccttcaaggg gtatcccctt tcgggcggcc tccgctacaa gccgagcctc
     3181 ggagtgaatt gctgtggaga gttcaagcgc cacacctttg tgaaaattat tgcgcggatc
     3241 accgtgaata tgcggcgagt gggcgcttgg cagatgctca ttgtatgccg tcagtattat
     3301 ttcaccattt ttgacgacag ccgctccaat gcgcctccaa aagtcagagc ttttctcgga
     3361 ttcctttttt aatttgctta ctacactttt atcaaaagct tcacgagata ttttttgatc
     3421 ggcctcgaca ggctttgctt ccattgtttt atgtttgtcc caacggagga aaaccgaatc
     3481 ccaggtgatg ccgcttttgc ttaaatattt ttgagccaaa gttcggctca catcctcatc
     3541 aggcataatg agtgcttccc cgtttatgtc gagaatattt ttctcatcta aaatatgaac
     3601 gtgtttgaaa aaaccaagcg cctcaataaa tttctttgtc acttctggag gcagggcatt
     3661 aatatcttta tcgatttgcc gataatgctt tattatctcg ggtccaaaaa tatacaaatc
     3721 tcgtgcttcc gttgcatgag actcaaaaaa ccttcggtat ccttcgtgaa taacaggaac
     3781 gtaagcaact atcactttat tcatacgatg gtttatccaa ggtcgaacct ttttttcgat
     3841 ttaaggttcg accttggatt tcattatttt ttatcaactg ccgacaggga ctccttaatt
     3901 acatcgagaa gtttaggagt tagcgtgcgt tcgagcttct tggccgtact tatttgaaga
     3961 agacgtattt tagcactggt agttgtcgtc gctttaggct cgagaactac tatttcttta
     4021 cagaatttct tgagattttt cagatctaat cttgaatatg ttcgcttcgt tgcgacaagc
     4081 gtatccggcc gtatcaattt aatgaggtgc caacgcggag ctttgtgttt tttgagatac
     4141 acaacgtcaa ctgatcttag atgagtcacc atctctagtc gctccgcttg aggtacaact
     4201 ggcctgtctg ggccttttcg ggcacgcact ttttcatcac tatcaacacc gataattaaa
     4261 atgtcaccat gtttcttggc cgcctcaaag taccgcgcat ggccgatgtg gaccatatca
     4321 tacgtgccca ttgtgagaac gatcttgagt cccaatccct tgcagttttc tacaaatttt
     4381 gacagtttcc tatcatcgcg aatccatcgc tcttcgaaat tggagcccgc accaaaaata
     4441 ccaataccta tgtgcgtgtt tagatttaac tctttcatag tttagctatt atatactgaa
     4501 gtcgaagaaa aatacggaga ccaaaaaacg aacgtttaga cgataaaact cgccgctaac
     4561 agacaaaccg acaaaaacac aaaataaaga tgccgtactt tcgcccgctc ataaagatat
     4621 ccggaatata tgacgagcac agcggccaca atctcataga gcaaccgagc attcgattca
     4681 acaaagagag aaaaagacgt gggccgagag agcaaaaaaa gcaataccag tccgaaaaga
     4741 tcgatcgaaa ctacgatcgc gcccattatt tggagcgtaa ctcctacgcg tttacgattt
     4801 ttttcaagtt tctcctccat cccgttagag acccgcctgc catcgcctga cacactgatg
     4861 ggaggacgtg tgattaattg gtaatatcat cttcttgtat taaagccact gtgtatttcg
     4921 ctctggctgt ggcgggcagg taggaaacgt cttgactaat gcggagccaa tcgtttgacc
     4981 tatgatttat tgataatgga gtcactattg ggaaaatggc gcggaaaaac gtttccgtaa
     5041 taaatcgcct gattttgatt tgaatattat attgtgtact caacatagta aaaaatacat
     5101 aacaataaga tctctaacgg gatcattaat ataactatac caaaagaaaa gacgcaatgc
     5161 gtcttttctt ttctacgact tattgagcgc aatggatctt cttgcatctt aagagaaaag
     5221 tgcgagtgtc ctacttcact tccagtcaaa aatatttttc tactgaaaaa ttttctgacc
     5281 acgcctcact cccctacggt cgttccggcc ggttgaggtt tctctacttc acctcaaccc
     5341 acgcataatt ttctgctgaa aatttgctcg ggcccgcctc ggctcgccag cctccggctc
     5401 gtggaagttt ctttatttca cttccacgcc catcgatctt gcagaacctt cgatgatttt
     5461 catggctgcg ttaatgtcat tggcgttcaa atctttcatc ttcttttcag caatttcctt
     5521 gattttcgat ctggaaatcg tgcctgcttt ctgaaccttg tttttgcctg atcctttttc
     5581 tgcgccgata gctttcaaaa taagtgcgga ggctggagga gttttgagta ccatgtcgaa
     5641 tgttctgtct tcatagacta aaacctcaac aggcacaata tcacccatca tgcctttagt
     5701 agcctcgtta aaacgggtga caaattcacc aatatttatc ttggcctgac caagtgcagg
     5761 accaactggt ggtgcgggat tcgcttttcc cgctgggatg acgagtttta atttttttgt
     5821 gattttcttg gccatgattg tttttcttac gaacgaagtg agttagaaac aacaatcacc
     5881 ccgttaaata ctctgtgtgt taagtacaac tcatactctc aaaaagtgcc taattagtca
     5941 attttcttga ttttcaagac ctatttaacg gggtcaagaa aatattagtc gcttcgcttc
     6001 tatatttttt taacttgcaa gaaatcaagc tctaccggag tttcacggcc aaacatagat
     6061 acgagaactt ttaccttacc gcgctcttcg tccacttcgg agacttttcc ttcaaggtct
     6121 ttaaatggtc cgtcaacaat aataatcggg tcgtccggct gaacatcgat attatgtttt
     6181 accgtttccg aattcattcg cttaaagagt atgtcaactt ctttcctctc gagcggtacg
     6241 ggataaacgc ctgaaccgac gaagccagtg acgcgcggag tgtttcgaac cacataccac
     6301 gaatcatccg tcacaatcat atctacaaga atatagcctg gatatatttt ttcctcttct
     6361 tcaactcgct ttccagcctt gatttttatc tttttttcag tcggaaccag aacgttgaaa
     6421 atcttatttt ccatccctag cgattcaata cgctgtttca gattgcgggc aacggcattt
     6481 tcataccctg cgtaagtatg aatagcgtac caatttcttt cttcttttaa tttttgcttg
     6541 gacatggttg attcatttat ccttcgattt gactcaggat aaccctgaat cccattgaag
     6601 ggttatgagc gtagcgaatt gataaatcac caccccgtgc aatataaacc tttaggttta
     6661 tatttgcggc cactttttgg ctcgcaaata tattgcgtgg ggtaaagaaa aatatgttcg
     6721 ctttgctcat tattttccta aattaaaaac tcaataacaa aactcgctaa ataatcaaac
     6781 aggcctagga ttgctcccgt tacgagagaa atagcgataa caataacggt ataaaaaata
     6841 gcttgctgtc ttgtcggcca actcacatgc ttcatctccg ctttggtttc tctaaaatag
     6901 tctataattt tcatcccgtt agtgacaggt cgcggtccca cgaattggga caatggcact
     6961 gcctattaac aatactatat tcctgttttt cagttttccc cgtctaccgt gaccgcggtc
     7021 actaacggga ttcatttttt cttactaaaa aaccccgccc gggggtattc actataatac
     7081 gcctttcgcc aaaagtgtca aataaataaa ttacagtcca cgttaactat aaccgcctaa
     7141 cagcattttt tctatttact aattcgcgcg aatgatataa tagagaaata agataaaaca
     7201 cctcacatcg ttttttgcca taatatcgcc aaacacaaaa cggctaattg ccagttttgt
     7261 gtttggggta agtccgcgtt cagtccgtat cagtcgcgtc tcaccgaatc tcgtacagta
     7321 tagtgacatt tgagctaatt ttattttctc cggtaggaat ctcgggggga gcagaagaca
     7381 catcggctcc tcgatccata gcctctgctt taaagtagat cgggtagttt ccagactcat
     7441 tgaaactaac aactcgtacc agtttaacgc ccaaatcccg agccagtctc tgggcttttt
     7501 ctcgagcatc atctatagcc atttttctcg actcattttg aggaactttt tcatcttcga
     7561 cagtaaagta aaggctgccc atatttgaaa ccttccgcga cccaagtcct tcgaggattt
     7621 ttcccgaatc atctatttta cggattttca gttgaattga atgcgtaacc gtgtagccaa
     7681 ccaatttcgg aacaccttgc gggggacaaa gaccattcgg aagacatggc tgataccgcg
     7741 cttgataatc atattttggg gattcctgat aatttattgt tttaatgtct ttctcctcta
     7801 ttccttcagc ctttaagtag gcgataattt cgttcatcct ctttgttaca tcttcctgcg
     7861 ccgcagacac tgtcgatttt tcggaaaaaa catcgaatgt aagttgtgct atatcaggct
     7921 taacaaaaac ttcaccttct ccggaaaccg aaatagtatt ttgctgtgtt ggatttttgc
     7981 ctacatagcc gatgcctttt atttcgccga tggttttaac cactagaaat atcgccacga
     8041 tgaagagtgc gaatcgcaga gcctgccaca tttttgtttt attttcgtcc atcccgttag
     8101 aaataggaaa cgttcaacgt atgttgctta ttcctattta taatgtaatt aattaataaa
     8161 ttttttgacc actttaaaat taaaataaga cgtttccgcc taaatttact gccgtaaaat
     8221 acaccgcaac tttatcgctc aaaccacgac tttctgcatt ccacgacagc agatttctaa
     8281 caggatccat agttttattt attttaaaca gctccaatag ggttattttt aaagcttagc
     8341 atagaggacg actaatgtcg gtaaatctta ctgggaattt tgagatacac gacactaaag
     8401 tatcccgtgt taattagcag aaatcgtaaa cggcgcatcg cttgaatcgc atattatttt
     8461 agtaacatct gcgtttcgat tacatacggt aattcgatag ttagttccga gaaccagggt
     8521 gcctccagct aataatcgtg ctgagggtct aaacgcgaca atgttactac cagaaatata
     8581 gctatctatc gacatatatg ccatgtcttc acgaccattt ttcaagtaaa gattaaaggg
     8641 accctcttgc aatatcgttt taaaagagat cgatatagta tcatccgaac gaaacgtttc
     8701 tcctccgttt ggtgagagaa ccgtaatgga aggattaaaa agcaaatttg cgttacagcg
     8761 aggataattg gttgcaccat ttgaacaagc cgttggggta gccggtttac tgcatccata
     8821 caacgcattc aattttgctc gggtagccgc tccaacatac ccagttcctc gagaaagcaa
     8881 gtttggcttc aaaatttcag atgcatattt ttcttggaaa ccagttacag ctgtgacgac
     8941 gaaaacatta tacgttagag aatcatcaat tccgctataa gcataaccag gaatatttaa
     9001 tccttccttc tggagcactt tccctagggc atccacgtcg gcccccttat caccaactcg
     9061 taaattccta gtccaattgt agcaaaaggt ggcagactgc ggagctggtg cttgaccacc
     9121 aagcgacgca cgtacgtcat ttataacttt tgcttctgca ccgaatgctt gaagtaagcc
     9181 aataacagca tcgatttgac tttgagttaa cgccgcgttc gctgttgatg cactaaatag
     9241 aaaacttcca agtaatccga gagtaataat tgttttagat atttttttca tagtctcacc
     9301 tttacattta cagatattct aatattccta ttatgcctca aaaaaagcgt cgtatatgtg
     9361 cacaactact tatgagtatg atcactaaag agtatacgat caaaccctaa taagcttctc
     9421 tccaaatcat ttattctaat tcgacggccg tcttttaata ctaaatgacg atataattgg
     9481 gctatcgcaa aggcaatcca aagtgcttac gaagccagta atcgttttct atctcctcag
     9541 caacaatgta gagaacgtta cgtccgaatt taaaatccag ttttagaagt tgaataaaat
     9601 cttcgcagtt atgcggatac acccacgcgc tatggtgtag tcttacaaaa cccaccttcg
     9661 aaagtgttcc gcgtaaaaga tttctattat ttcggttctt ctcgcttatg tcgaaactta
     9721 aaattctcca cttcccatcc cagcgtcgtc tagggataga aaatttctct aactctcgaa
     9781 ttcgctttat atacaaatcg ccttttttag tgagagcaca agtgcgcatt ccctgctctc
     9841 ctgaaatcac aataaaacca gactctgcca gtcgtcgaat cgactgataa gcactcgagt
     9901 tacttgtttt gtatctgaag agcttaagca tgtttggagc agccatagcg agagctagac
     9961 ctccaacggc cccaacagtc tcgagcaata ttttttttat cctcgtcttt cttacccgtt
    10021 tttcaatcac tcccataaag aatacataaa aattatgaaa agtagctacg acatgcaatt
    10081 tattctaaca tgacggccgt cgtattaaaa taaaaagaaa catgttgaga ttgaattgga
    10141 tgaaaactaa gtcacaattt tagttttact ttgaattttg ctgaagcgtc gccgctaaaa
    10201 ctaagttttc gaagagcgca cagacggtca taggaccgac tccgccggga actggagaaa
    10261 ttgcctcgac tataggttcg acgtcggcaa agttcacgtc gcccaccagc cggcgtccag
    10321 cgatttcttc gagatggcct ccttttttct cgtttatgcc gacgtcaatg ataacctgtc
    10381 cagttcttac aaaattttta tcaataagtc ccggctggcc ggcggcggcg attactatat
    10441 cggcgctttt ggatatttcc tgaagattag aggttttact gtgacaaatt gttaccgttg
    10501 catttcgatt caaaccgagc agtccagccg gcttgccgac caaataagac cggccgataa
    10561 tcacaatgtg tttcccttcg accggaatac cataaaaatc gagtaacata aaaatacctt
    10621 taggcgtggc cggaataaac ccgcgttcgg cgcgcagaag ccggcgcatt gattcaccgg
    10681 tctgaccgtc aacatctttc gatggatcga ttttttcgac aagaagatag gcgtcaatcg
    10741 acgggggcac gggaagttgg aggattatgc cgtttatttt tggattttca tttagttcgt
    10801 cgattttctc ttggatttca gaagttttcg attcggtagg acattgtatg tggatcacta
    10861 ttgcaccgat ttcttttcca aatttctttt tcgcctcaat atatgcagac gactccgcgc
    10921 gatctccaac ttggataatc gcgagtgtgg gctttgcacc catcttttct atttcggctt
    10981 ttagactaag agcccgtgcc tgtcttgcca ttttgccatc aagaagtttc gccatataca
    11041 aataatacag ctaacatacg aaattaatat caattataca aatctaatgc gttctcttca
    11101 ttagtattat tggtacccat tcgtatattg gcatttctac aaggaaaaca atagcacaac
    11161 ggcaaggagc gtcataatga cgtcctctcc taaaatccta gccaaaaaga tcaaagcgtt
    11221 tgtaggacat ggcaaaacta ttccactccg tcaatttgag cgtgcctgaa attattaaaa
    11281 ttgttcctaa gacgaattgc atacagcccc cgccggacgg cgggggctgt gaatgggctt
    11341 ataggccaat atctatatct ataggcggtt tcggttctgg ctcatcaccc tttttatccc
    11401 gcttgtccgc cttgattggc cgatcagctt cctcctcttt agtctcttcg tctgaggagt
    11461 ctgtggtggt cgatacaccg gagctcgatg ttgcgttcaa cataagcgct gtatcggcag
    11521 caatgcttgc gtttacctct ctcatgacgt ctatccccag ctcatctttc acccgcacag
    11581 ctaaccgtgc gccctctgct tttttctcgc catttttaaa ggctaccact gcattattgt
    11641 aggcatttac actgacatat gcctcaccct cggcataatt ttccgatgca tgatcgagtt
    11701 ctatcgttac ggcgccgttt acttttttgc tcttcgcaaa gtccctcgtt tttttaattc
    11761 ttttctccgc cttctttcgt gccgcctcgg cgataggagc gctgttaaat ttcgcatggc
    11821 tgcgcactgt cgattcagca tcaattctgg cctcggcggc cgatgaaact tgagcattta
    11881 ctgatgaaga aataccgtcg agaaccgagt tgtcggtttt tatttgggcg agaactgtgt
    11941 catgcacagc aaatcccgat tccattttcc cagcgatatc aagcgcatct tcgtggccat
    12001 tattgctacg aagacgagca acgtttactc tcgccgattc tgaatgcgcg acaaaatctc
    12061 ctcgaatacg ggccgacgtt tccgcgtcga gctccccctg aacggccaat ttttctgcct
    12121 cctcgagtcg acgcatggca agatcggcat gtagttctgc ctctgctttt gccccgatcg
    12181 caatccaacc tgctacgcgt tcatttacac ctcgcttcaa aggatacagg aattcattgg
    12241 ggagcgcgtt tgaagcaagt gcacttcccg taccaaaaag tatgagcaca ataagcgtga
    12301 gcgttgcagc actataccga gtaaaaaaaa gggagaaacc cgcccatata aaaatagatt
    12361 cttttccttt tcctttgagc gggtgcgttt gcatgaaaaa aaggatgctt tctttaatgc
    12421 ggtccttctc acttgcggag agcgaaatat tcttttttcc ggggactggg tccatttaaa
    12481 ttttaagcaa atcttgaatc ttttttatgc ttctgttgat gcgaacggaa accgtatttt
    12541 cagtctcccc gagtatcgca gaaatttcac ctagagtaag accgtcgatg tatcgcatta
    12601 ccactatctc tcggtactta ggaggaagtt gatctatggc ttttcgcatg tgttctgttt
    12661 ccgcttgctt gacgatgctc tcatggccgt caacttcggt agatacatca aatccgtcgt
    12721 ctctcataac atcaagcgat accgatttct tttttctgta ttcgtcgatg ataagattgt
    12781 tcgcaatctt ataaataaaa gacttaagat tttttatctc tttgccctct acgagatatt
    12841 cccagctgcg ggtaaatgtc tcttgaacca actctttgga tctttcgcga ttagatagtc
    12901 tgaaacaaaa ccgtctatat agtgggtccg ccagctcgtc ataggtcttt atgaagtttt
    12961 cactaatgtc ctcgtttggt gttcgcatat gaaggacgaa ataagcttaa ctttcttact
    13021 gccgaaatta ggctatctaa gaagttaata atctgaaaaa attaaatttt aaaaaacacc
    13081 caaactctcg ggtattatct taccctactc ttttaaaaca tatgaaacat cgactcaaga
    13141 ttttccggcc ttttctgtcc tcatttcaaa tataacagcc gacctttttt tgtgtacttc
    13201 catatgcact attaccatgt tctagtgctc ttcagcttga acacttgaga cgcacagttg
    13261 aaaagcaaag ctatagatgt tccgagaaaa aatccccctg ctatgtctgt aaaccagtgc
    13321 gcgtggagat acattcttga aatcaaaacg gccaagcaga ggacaaaaat tgcactattt
    13381 agaaatattc tagtcccccg ccgaggaatg cgcccgatta tgttggttag gctaaaaaac
    13441 aaaactgccg tcagaatggc gtggccggac gggaaactga agcccgacgt atcgacgagc
    13501 ggaccaagcg gtcgctcgat ttgaaaaaac gacttcagga tctcgccgac agacgccgcc
    13561 gcgacaagcg agactgcgat tgaaaacgcc tctgtctttc tggcactgaa gtatgcgtgc
    13621 gccgctgcga gcagtacaaa aattccgatc actttcgtcg agaaaagaaa tgcgattatc
    13681 ttgaagaatg cgtctgctgt gtcggattgt acgttccaaa acaagaggtc aattgcatcg
    13741 ttcaatcgcg cgacaaatgg aaacgcgagg aagagaacta cgccgcagaa aagcgcatag
    13801 aaagcaaacc cgattaaaaa aagatttttt tgtctcgcgt cgaacagatt actttttcgc
    13861 atatacccgt agaaaccggc aagcgctatc aataatagaa ttaaaatata ggtcacgatg
    13921 aaaatattta cgaactactg accgttgatg tgcaatgggg acatcttgtg gcctttttgg
    13981 ggatgtcgag acggcagaaa ggacaggcca tgagctccgg agcgactttt gcttcttctc
    14041 tacgacgaaa gcgattgatt gcccgcacaa ccatgaacac tgcgagcgcg gttaagagaa
    14101 aactaataag cgcgttgatg aaaagcccgt aattcagcgt tggtgcgccg accttttgcg
    14161 cgtcagcgat tgtaccaaac cgctcaccgc ctagatttac aaaaagattt gaaaaatcaa
    14221 cccgtcgcag taaaaggcca attggcggaa gtaaaatatc gttgacgagc gaattgacga
    14281 ttttattgaa cgcggcgcca atgataacac cgacggcgag atctatgacg cttcctcgga
    14341 ccgcaaattc tttaaattct cttaaaacgt tacgcatttt aaaatgaggt gcggtccccc
    14401 gcgttcatcg ccctaaattc aatctcgtgt tcggccaata tttttccagg aacgaggtgg
    14461 tgagtcttgg caatcggttt cagcattccg tcatgcaccg gaaatgcgat tttgggcttg
    14521 atgataaggg cgtaatcaat ggcttcagaa attttcatcc aaggacccgc aacgggaagc
    14581 gccaagactt cgacaggaat tttaggatct aaaaaagcat cacccggata aaaaaactta
    14641 ttcgcgatga aatagccagt attaacgact tccttccaac cgggatatat aagtgcatgt
    14701 ttttttccgt gccccgcgat agtgacctct cgttcaacgg tcgattcgcc gtcctcaagt
    14761 accgtatatg aaattttctc cgcatcaaga atcgcagcca cacccctgtt cgtgaggacg
    14821 agggcctttg gattattttt cagaatcgcc ctaagagaag ggatgtgcag atgatccgca
    14881 tgctcgtggg taatcaaaat aacgtcgaga tcacgcacat cactttggcc tgttgaatac
    14941 gaaccggggt cggtcagaat gcgcagacca ttttcctcaa ttacgagaca gcaatggccg
    15001 attttagtga tgatcatgaa agatatggct tgcaaaataa aaccaatagg ccgcaagcat
    15061 ggcacccaaa atcaacgtca catgaaaaat gtattctaca tagagcaaat gttttaagtt
    15121 catcccgata acacggtgaa agttatcatg cggccaccac gaaacaagtg cccacactat
    15181 atagccaaag gcccaaccgg ccggttgtcg ccatatccca aaccgccaga tatatactct
    15241 tccccagaca acaaaagaga caccgacacc aaatgcaatt gattcgatga tgtcgagaat
    15301 aatgaagaac ggaataagcg tactcgacgg actctgcatt gatggatcat ttggccagat
    15361 gacatgcgta agcagaaaag tcgcaactgc cataacaaca gttagagtga tgaattttgt
    15421 cttcgtcttc atatggttaa taagctctct ctttttccag aagaattatt ttattttttg
    15481 ttcctctatt ataacccccg gcagaaccgt cagagcgaac tactctgtgg cacggaatcg
    15541 aacgcttata attggtattt aaaatattcc cgacctctct atatgcgagc ggacgcccgc
    15601 acaagagtgc cacttccttg tacgttaaaa cagaacctcg aggtattttt gagacaatac
    15661 taaacacttt ttccttgaac gaccctgttt ccatatatac attgtagcga atctaaatcc
    15721 acaaaccaac aaaccccgcg agttcacgaa ggttgtcgca tgggatcgtc tgaaaatcac
    15781 acgcagacgt aggtgcacat aaaccttcct ctacttcaca atgtatgtta tggtctcacc
    15841 catatatccc gaaatttcta tatgcctagg ctataagaaa cgaaattcat ctgctaatag
    15901 tttaaagaga aagataatgc tgaatgtttc gtctactaaa aagttacagc tagataaatt
    15961 tattatatga aaacaataat tttagctgca gggtatgcaa ctcgtttgta tccgcttaca
    16021 ctcaccaaac cgaagcccct attgcaggta gcccataagc cgatgctcga gcgggttctc
    16081 gagaaattag cgcttgccaa agatgatgaa gtaatcatag tcacaaatga aaaattcaga
    16141 ggcaactttg agcgcttcgc tgattcgtac aagcgaattc acgggatttc attaaaagtg
    16201 ctgaacgacg ggtcctcgtc cgatgaaaac aaactcggag ctatcggtga cctccacctt
    16261 gcaatcgaaa aagaaaatat tgccgaggat attttagtaa ttgccggaga taatctcttt
    16321 agcgaatcgc ttgctccatt tcgggaatat gccgtgagaa aaaacgctcc cgtattggct
    16381 gtatatgata tcggtagtat agaagccacg aaaaaattca acgcaatcag tgttaatgag
    16441 gaatcggtaa ttaccttttt tgaagaaaaa ccttctcggc cgcaaagctc gcttatggga
    16501 attgccctct attattaccc gaaaaacatc gtgccactaa ttaaaaccta cattcaagag
    16561 ggtaacaatg cggaccagcc gggacgcttc atccagtggc tctacaaaaa aatgccagtc
    16621 tatacgtggc gcgttcctgg aatctggttc gatatcggct cgaaagagac gctcgacgaa
    16681 gcaaaccagg ctttcgccga aatcgctctt gagaaaaaag gagcgcatgt cccagaacta
    16741 gaatacgaag tttaaatttc gccttttatg ttacctctcc gaggagagga atgtttaatc
    16801 tgcgtcgcgc agattctccg taaaaaagat tccttttgcg cagtgctttg aatccccgat
    16861 cgccgtcatg ttggggagta ttcatggcgg caaggcttga ggcaagaaaa acggtgtgtt
    16921 tcccgtgtcg cagagcgatt tcatcaattt tttggtggac taaggagact gcctccgctt
    16981 ttatgcatcc gccaaaaaga tccggctgac ttatttcagc accttggagt tcaaacaaca
    17041 cggcacccgt ggcgcgatag agagtcttct cttgccatac ctcatcaaaa taccttcggg
    17101 ccaacggcac aagttcttca ggaagcgcgc ttgtatgaga caacttgaga tcaaatccat
    17161 gataacgaaa ctcttgtgtc ttaaggtaaa aagaaaattt agcggccacc agtttgtgcc
    17221 gtcgcgcctt aatgcatgcg ttttcgatat tcttactaag ttgcgaaaac acaaatgctc
    17281 gatctttcga aggaggtgta aaagtacgcg ttttgctgat tgattggtac tcatgctttt
    17341 ccgcagtgtt aagtttataa accatctcac cgcgaagctc gagccatatt tcctgaaagg
    17401 gttttgaaag atgctccata atccaccaat ttggtttctc gataaactga agagcggtgt
    17461 ggactccttt tttttcaaga aacgccgccg tgttttctcc tattccccaa atttttgcgc
    17521 agggcaaatt acaaagataa ctttcaattt cattttgaga aatgaccgtg agcccgtcag
    17581 gttttttcca ctttgatgcg actttagcaa gtacttttgt aggcgccacc cccattgaaa
    17641 atgtgagacc aagctctgtt tgcaaatcat gctttatttt tgctgcaatt tctccataag
    17701 aacagcgaaa ggttcgtcgt aaaccagaga gttgcgcaaa acattcatca atactgtatt
    17761 cttcaacaat cggtgtatag cggcgcacaa tttcaaacat ccgttttgaa aaaagactat
    17821 aggtttcata atcagatggc aaaataatcg cctcggggca cacgcgacga atttcaaaaa
    17881 gtgacatgcc gcgctttacc ccccgcgcct tagcctcata tgtcatggcg cttgctatgc
    17941 cccgctcttt tcccgtcacc acaggtttcc ctcgtagagc tggattttgc gccacctcgc
    18001 aagaagcgaa gaacgagtct ccgtcagtat gaagtatggc gtgtgaaaaa tgattccagg
    18061 acatgtgtta agcgtaaagc gtaaagcgaa tagggtttag ttaattctaa acgctagacg
    18121 ctcaacccta aacgcttctt atataatagt gtacgttcgt tcacttgtcg aggaaacaaa
    18181 aaaacggcca catgggaccg tttgaaatac aaatttcgga aagctattcc acacccggaa
    18241 aaccgtgacg gaggagagac gccaataacc cgagcggaag accggaggaa tcttcccgcg
    18301 aagaatgcga ggagaccgtg actctccagt cagaagtaac acccaaaacg tcacctttct
    18361 tttccgcctc cggaaattca gccctgagtt ccatccaaaa aagcttggcg gccagctcgg
    18421 aaagagcctc gatttctgcc ctctcgtgac tccacttctc aacgtcaccc ggaacacgag
    18481 gggcgggacg caggagcgcc tcattacgca ggcgggtacc gagggagaaa aactccttgg
    18541 cccgctggga agagagaacg cccaccagct gatccttcaa aatatcggct tcgaccagag
    18601 gactgtagat ctccgcctcg ggcgtaatac gacccgcata gatggccgcc aactcatcgc
    18661 ggaaacattg ttgacgcgtc ggcgtctttt cgggagctgg cggcggagtc tgtggcgagg
    18721 actgtggcgg gggcggtgct actgcgggct ggggattgga ttcagatgcg gggcctcgag
    18781 cgaactggcg ggaacgtgtc gttgttttca tcggtgttag tagtgcagta ttttggttta
    18841 tggcttcatt ctactttgtt ggagaactat gccacgagta aacatacaag tatgtctata
    18901 gtcatgtcaa acccacatat acttcaacga agacgtcttt cggggattat taatacagat
    18961 tgctgtgagt atcgatgtca gtaaagacta ttgattctgt agttttaaaa taatagacgg
    19021 cgcggaggtc accggtgata ttgatactcc aatagccatt caatttaccc tgcaatgaat
    19081 gattgtttaa cgttggattt tgaaaatcgc tttcgaaaag ctctaaacgt ccgaggagtc
    19141 gagcgactac ttttgcgcta agtttctttt gtttctttac gaagttctta ttgaaaagaa
    19201 taatcatgct ctctgttttt ttagatattt gatcgcatct ttcatattcg taaaagcagg
    19261 cgatagattt cttccacgct taatatcatc ttcccatttc ttgagctttt tctcgtatcg
    19321 agccgacagt ttttgaggcg tatataaatg aacttctttc gtggcaataa actgctgtag
    19381 ataggcattt atcggtacgg atagtggaac acctagctct cttgcgactt tctgagcttc
    19441 tcgcttaagc tttggatcag tttttatatt tatgattgat gttttcatgg ggtagtgaaa
    19501 ttttaatttg ttaaatcctt cgacttttgt tcataaaaaa attctttaat tctccgtttt
    19561 agtaatcttc tgccttgagt cgtcttgaag taccgttctc gtcttaaggc atccttttta
    19621 ttcatgcatg cctcataata aattagtatc cacggcatat acctctttgt cgaaggattt
    19681 aatccttgat tatgttctat gagtcgtcgc ttaagatcaa cagcgaaacc aatgtaaatt
    19741 tcatcgttgc gttcactttg caagatgtat gtatagaaca taatcgaatt gaaattctac
    19801 taccccattc atattccaag tatacacaaa gtatatacat tgtaaactat taatcaatag
    19861 ctcattctga tgctttccta aagttgtaat ctttgtacag atccggttct tctttaagaa
    19921 agtcaaagct tccagaatag gcattcattt tggtaacatc attgaggcgg ttatcaatgc
    19981 tcaaatcctt tttgtcgttt tctgaatctt tgatcatagt tttattttac caaagatggt
    20041 tcacaataaa taaggaaact taaaaactta ccgacttaac aacttataaa ctaatctaat
    20101 acttcctcac cacagctctc accacagcag caatcttgag atcatttttg gggacaagag
    20161 gtttatattt ttcgttcgcc gcctcgagat agatgtgtgt tccacgttta cgaagatatt
    20221 tcatcgtcca tgctccgtcc acttcagcaa tcacaatatc ccccgccttt ggactgtttg
    20281 tgcgctcgac gatcaccata tccccttcgc aaatacccgc atttttcata gaatcgcctt
    20341 taactttgag catatagctt gcttccttgt ttggaatcaa atattcatcg agactcatgg
    20401 tatcggcaag ttcctcttct gcaggagacg gaaaaccggc ctccacgagt ccaagcagac
    20461 gtaaatcacc gaaaatattt tgcggaatga gcttgccctc tttatcttta gaaataaact
    20521 tcaaatcaat aagcttttgg gcgagtttgt atgccgcatt ttttgaccta aatccaagaa
    20581 gcaccgcaat ttccgcatag ctcggcagac gtctgtgcgt tttataaaaa tgagtgattt
    20641 tctttttgta agattccata caaaaataat tttcaatccg tcagctgacg gatcaacgat
    20701 caattttcaa caaaagcacc gatcttaatt tttgaaaatt gcaaaattga taattgatac
    20761 tttattttag tgtaccttcg ttcactttgc aagccaacaa aaaagccgag ccgcaaaagg
    20821 ctaggctgca aactcaaatt cgagctgaac tgttggatct aacctgtagt tcagatagtt
    20881 ccaggcaaca gcgaccgcaa gagcccctgt acaccgataa aatctgatca gcatctcgtg
    20941 tcgagcgaag tgatccatcc gcctaagtcg agagacgagg acattgatgg ccctctgtcg
    21001 aaagataagc cagccgacga aatcctcgat caaccggtgg atgggatgaa gtccccgacc
    21061 agcccaggga taaggacagc cgatgactct gattaattgc cgagtcgtga gtcgcgaaac
    21121 attgattttt gttctcatac taaaaaatat aataacgata attttttgtt aagtcaagat
    21181 tgcgacttcg gctttgcctt cgcctttggc gaaaagaaga tagcgctcac ttcagctcaa
    21241 gtcccatcta ttttgtgaaa tgctataatt gctagtgagg gagaaatgaa tatgtcttta
    21301 tattcatttc gaacgaatga cgcaattata tgtgactcca atcatgtaat tttaattgat
    21361 gatgccgaac tctgaaaaat ttgctcgttt tgttctcttc accatatata tttggtttgg
    21421 tctcttgaaa atttggggga caagcccggc aaatccactc gtcgaagcac tgcttagtcg
    21481 aactctgcct ttcgtatctt tcccgacatt catgcttttc ctagggacct ttgaggtcat
    21541 tattggatta cttttcctta ttccactctg gactcgggcg acaaaaatca tctttgccat
    21601 tcatatggcg gttgtttttt tgcctcttat ctttcttccg gcaatcgctt ggcaaggtac
    21661 atttgtgccg acactcgagg gccaatatat tttaaaaaat ctggcactca ttgccctagt
    21721 tgcaggatta tagtttaaag tggttatatt tttccagttt ttagaagcac ttctatttcc
    21781 acaggaattc ttctatacag tcgagtctct tggcctcttc tgcatctacc catttaaaat
    21841 ctgtgaacgc gcctcgctcg aggagaacct ctcctccttt atagggagcg tcaaatttaa
    21901 ccatagccac gggaattccg tccggtctga caaaagcaac ggaatttata tatgtgagtt
    21961 ttggttcaat ctctattccc gcttcctctt ttgcttcacg cacaagcaaa tcctccactg
    22021 tattctcaaa atcaagcaca tcgccatttc gccgagaagg cgcatcaagc ggcaaatcac
    22081 tccaccccaa ttttcctccg atcactcccc atttactcgg atgaactttc tcgcgtaaat
    22141 cgcgttttaa aagcaaacat cgcccgtctg tttcccgaaa gacaatcaca ttcgccacaa
    22201 aatagaatag cttgtctttt tttgcgtttt caagacttat ttttttcgcc ggcattattt
    22261 tttgagcttg tttgcttcct gctcaatttg atagagttcg tgctggtgag tgcgttcagc
    22321 actctcaact ttcgccgcct cccgttcgag ttcttttatt ttttcggtta gagtgtttat
    22381 ttcttgacgc gacccgatta cttcggcgtt ttttcgttcg agttcgctga ttttctgctt
    22441 aagcgcatcg acttcacgtt cgcggcttgt taattcagtc ttttttcgtt caagctcact
    22501 ttttttattg cggatttcat tgtagaagct ggagactttg agccgttcgt tatcaatttg
    22561 ttttttgagt tcgcctgaca tgcgtgaagt atcgcccata gtaggtagat tatatcacgc
    22621 gcgtatagca gtgaacattt ccagtatata gtggtgtata atttgctgat gaaaaagtcc
    22681 agggcaggcc aagcgaaaaa gctcttaatc ttgttttgct ttgcgctgtt gccttactat
    22741 ttaaactttg aattacaaaa ctctctcacc t
//
LOCUS       DATDSU010000114         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1298965, whole genome shotgun sequence.
ACCESSION   DATDSU010000114 DATDSU010000000
VERSION     DATDSU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298965"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VI981_04040"
     CDS             <1..232
                     /locus_tag="VI981_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479498.1"
                     /translation="MGIERSVPPEFGGEFFRRMRESVEIGDVPETPADEDKLIIDAAW
                     ENNLKKTMNTGTSAMAEWEKEKEKKEDEQAKR"
     gene            252..1418
                     /locus_tag="VI981_04045"
     CDS             252..1418
                     /locus_tag="VI981_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY4479499.1"
                     /translation="MENQENTKKSDGINKLWIFLLIIVLIIAAGTLFSIAKPNKSPYR
                     PELASDVKDISANATDVFPTADARDNKAVLIKLKATQVVSELAPGVSYEYWTYNSQVP
                     GPFIRVKEGDTVEIELTHEHAHNVDSHAQADSEPTFLVNFIPTVFADTEGDNVDGHMM
                     ANDTAEHMAAGHGEHSIDLHAVIGPGGGAEATRVGPNQTKTFKFIATRPGIYVYHCAS
                     PHVPTHIANGMYGLILVEPKAGLPPVDKEYYVVQGEFYTKGRVGDKGHQEFDKDKLLN
                     EKPEYIVFNGRKGSLSGDRALHAKVGDRIRIFFGVSGQLASNFHVIGEIFDKVYPEGD
                     LVSPPHKNIQTTLVPAGGATMVEFTAEVPGKYLLVDHALTRAVDRGALGELIVE"
     gene            1469..1541
                     /locus_tag="VI981_04050"
     tRNA            1469..1541
                     /locus_tag="VI981_04050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1502..1504,aa:Ala,seq:tgc)
     gene            1801..1876
                     /locus_tag="VI981_04055"
     tRNA            1801..1876
                     /locus_tag="VI981_04055"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1834..1836,aa:Ala,seq:ggc)
     gene            1915..1985
                     /locus_tag="VI981_04060"
     tRNA            1915..1985
                     /locus_tag="VI981_04060"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1947..1949,aa:Ala,seq:cgc)
BASE COUNT          620 a          444 c          548 g          551 t
ORIGIN      
        1 tatgggaatc gagcgaagtg tccctccaga atttggcggc gaatttttca gacgaatgcg
       61 tgaaagcgtc gaaataggag acgtgcctga aactccagcc gatgaggaca aattgatcat
      121 agacgctgct tgggagaaca atcttaagaa aacaatgaat accggaacat cagcaatggc
      181 agagtgggaa aaagaaaaag aaaaaaagga agatgaacag gctaaacgat agaagaaatc
      241 tgcataggaa aatggaaaat caagaaaaca caaaaaaatc agatgggatc aataaacttt
      301 ggatattttt attaataatc gtgctcatta ttgccgcagg cacacttttt agcatagcaa
      361 aaccgaataa atcgccgtat cgtccagagt tagcttctga tgtgaaggat attagtgcaa
      421 acgcaaccga cgttttccca acagctgatg ctcgtgacaa taaagctgtt ttaataaaat
      481 taaaggcgac tcaagtggtg tcggaacttg ctccgggcgt gagttatgaa tattggacat
      541 acaatagtca agtgcccgga ccgtttatac gggtaaagga gggcgatacc gttgaaattg
      601 aacttaccca tgaacatgcg cacaacgtgg attcccacgc gcaggctgac agcgagccga
      661 cttttctcgt gaattttatt ccgacagttt ttgcggacac agaaggagat aacgtagatg
      721 gccatatgat ggctaacgat accgcggaac acatggcggc tggacacggc gaacattcta
      781 tcgatcttca tgctgttatt ggaccgggtg gcggagcaga ggcaacgaga gttggcccaa
      841 accagacgaa gacttttaaa tttatagcaa ctcgccctgg aatatatgtg taccactgcg
      901 caagtcctca tgttccgact cacattgcaa atggaatgta tggcttgatt ttagtagaac
      961 caaaagcggg tcttccgcct gtagacaaag agtattatgt cgtgcagggg gaattttaca
     1021 caaaaggtag agtgggagac aaaggtcatc aagaatttga caaggataaa ttgctcaatg
     1081 aaaaacctga atatattgtg tttaacggca gaaaggggtc actttcggga gatcgcgcgc
     1141 ttcacgcgaa agttggtgac aggattcgca ttttctttgg agtgtctgga cagcttgcct
     1201 caaattttca cgtcatcggc gaaatttttg acaaagtata cccggagggt gatctagtat
     1261 ctccgccgca caaaaatatt caaacgacac ttgtgccagc tggaggggca acaatggttg
     1321 aatttacagc tgaagtacct ggaaaatacc ttcttgtcga ccacgcactc acgcgtgctg
     1381 ttgatcgtgg tgcactaggt gagcttatcg ttgagtaaaa atcatttttt gcttagaaac
     1441 aataattgtg tagaatacct ccattcatgg gcccgtagct tagttggtag agcgcctcat
     1501 ttgcaatgag gaggtcaccg gttcgaatcc ggtcgggtcc acaattctga atttgatgaa
     1561 agattgacct cgcccgcgcc tttggcgcac ttggtttcga ggagaaaccg aggttcagat
     1621 atcgtctccg gctgaccaag taagatcgag attctacaag aatgtcgatc ttttttattt
     1681 tggctagcag gctttcctgc cggcccgata ttagcaatag gtgtttgggt ggggtgagtg
     1741 cgattgccgg tttgatcacg gctgccccga taattaaatt tatattacaa taatatccct
     1801 gggccactag ctcatttggt agagcgcttc catggcatgg aagaggcgac cggttcgatt
     1861 ccggtgtggt ccaccaaaaa aatttacttg atggtatagt atttcaatac ttttgggcct
     1921 atagttaagt ggtaaaatgc tcgattcgca ttcgagaggc gcgagttcga ttctcgctag
     1981 gtccacaacg tacaacaaat ctgccgcagg gcagattttt tgacgttgtg gtgttattct
     2041 agcatttgcg cgaacctttt ttgaacgaaa ggactaatcg cacgcgccgc gtgcgtggtg
     2101 gcttgggact gcatcatacg ctccgattgc gctggaatga atcccgccac gcgcggagta
     2161 ccg
//
LOCUS       DATDSU010000115         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1943534, whole genome shotgun sequence.
ACCESSION   DATDSU010000115 DATDSU010000000
VERSION     DATDSU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943534"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..851)
                     /locus_tag="VI981_04065"
     CDS             complement(90..851)
                     /locus_tag="VI981_04065"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEY4479500.1"
                     /translation="MRRINSIVVLFLFLLIAVLAAIGFSREGNEGINFRETGNIVLGG
                     AGRLPSVWYLIYEEPDAPSLAFQLVFNDTSKCEKNREVIECGLTLSNGARAEIGGFRE
                     KDILIVKDLLIELEGEPLSDTLPVQLFYYDAKKDKDALGVILCSAKGLVPVERKVPNE
                     NSIEETIKLLIHGELTQLEKKAGITTEFPLAGLTLANSSLQNGTLILTFNDPQHKTSG
                     GSCRAAVLSHQIIETARQFPEVSNVKMLPEELFQP"
     gene            complement(917..1501)
                     /locus_tag="VI981_04070"
     CDS             complement(917..1501)
                     /locus_tag="VI981_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479501.1"
                     /translation="MNDNPNMEAGNVPQGSMRKTIGIIIVVVAVIILLILLMGRGEDK
                     TPTGQENVASESSNPAPQNESSFSGTFQELAMRGGDYMCTFDSGAAGGNVSGTMYVSG
                     KNIRGTFKTTGAGAVESNMVMDGNYSYVWTSAYPQGFKTKIDQTASTQTSASSGQNSV
                     DANAKYDYKCQSSKVDAAMFTLPMNIVFNETPMR"
     gene            complement(1568..2251)
                     /locus_tag="VI981_04075"
     CDS             complement(1568..2251)
                     /locus_tag="VI981_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4479502.1"
                     /translation="MIQTIKLTKSFKNGDIETKVLKGIDFSATEGQFVAIMGRSGAGK
                     STFLYQMSLLDSPTGGDVILNGTNTKNMGPDEKMKFRLIQFGYVFQDYALLPDLTALE
                     NVALPLLMQGFSRRDANKKSKDVLAKMGLAHRVDNLPSQLSGGEQQRVSITRAVAHDP
                     KILFADEPTANLDNESSRKVIEIFKELHKEGQTIIMVTHELEYAKEAQIIVHLDDGKI
                     TEITKNRSV"
     gene            complement(2408..>2608)
                     /locus_tag="VI981_04080"
     CDS             complement(2408..>2608)
                     /locus_tag="VI981_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479503.1"
                     /translation="LVYAFLVPVIADHPINFPFSDGILVAPLDQTLFRVGLLVVSTII
                     AGYIPAWMIIRKNTLDSILGRN"
BASE COUNT          697 a          604 c          527 g          780 t
ORIGIN      
        1 aaaaaacctt acggagggcg ttgagcccga gtagagaaat cgccagcagg cgattatcgt
       61 gtttttttaa ataacaatct tcgcgccctt tacggttgaa aaagttcttc ggggagcatt
      121 ttgacattgc ttacttccgg gaactgtcta gccgtctcaa taatttgatg agagagcact
      181 gccgcgcggc acgagccgcc cgaggtctta tgctgcggat cattaaaagt tagaataagc
      241 gttccatttt ggagagacga attggcgagc gtaagtcccg caagcggaaa ttcagtcgtt
      301 atccccgcct tcttctcaag ttgagtaagc tcaccatgaa taagcaattt gatagtttct
      361 tcaatactat tttcatttgg aactttccgc tcaaccggca caagcccttt tgcactgcac
      421 agaattacac ccagtgcatc tttgtccttc ttcgcatcgt agtagaaaag ttgcactggt
      481 aatgtatcgg aaagcggttc gccttcgagt tcaatcaaaa gatctttcac aatcaaaata
      541 tccttttccc tgaaacctcc gatttccgcc cgagcaccgt ttgaaagcgt cagtccacac
      601 tcaatcactt cgcgattttt ttcgcacttt gacgtgtcgt taaagacgag ttggaacgcc
      661 aacgaaggtg catccggctc ttcatatata agataccaaa cgcttggcaa tctccccgct
      721 ccacctaaga ctatattgcc tgtttcgcga aaatttattc cttcattgcc ttcacggcta
      781 aagccgatcg cagcaagtac ggcaataagc aaaaacaaaa acaaaacaac gatactattt
      841 atacgtcgca tgaccttatc ttactatttt gggtcaaaaa aaacacactc gcagagtcgg
      901 gggtgttttt aaaaacttat cgcatcggtg tctcgttgaa tacgatattc attggcaaag
      961 tgaacatggc cgcatctacc ttcgaagatt ggcatttgta gtcatacttc gcgttagcgt
     1021 ctacactgtt ttggcctgac gatgcagagg tttgcgtcga tgccgtttga tcaatttttg
     1081 ttttgaaacc ctgtggatat gccgaagtcc aaacatagct ataattgcca tccatgacca
     1141 tattgctttc aacggcccca gcacccgtgg tcttgaaggt tcctcggatg ttttttcctg
     1201 aaacatacat tgtccctgaa acatttccgc cggccgcacc ggaatcaaac gtacacatat
     1261 agtctcctcc tctcattgcc agttcctgaa acgtgccact gaaagagctt tcgttttgag
     1321 gagccggatt cgaactttca gacgcgacat tctcttgccc cgtcggcgtt ttatcttctc
     1381 ctcgacccat caagagtatg agcaggataa taacggcgac gactacgata ataatgccga
     1441 ttgttttacg catacttcct tgaggcacgt tcccggcctc catatttggg ttatcgttca
     1501 ttacattagt ataaaaataa taattttgtt ccccagtgac taaatagtat cacagcgttt
     1561 ttgcttttca tactgatcga tttttagtaa tttcggttat tttgccatcg tccaaatgta
     1621 cgataatttg ggcctctttc gcgtattcga gttcgtgcgt gaccataatt atcgtctgac
     1681 cctctttatg cagttctttg aaaatttcga ttacttttct cgaggattca ttgtcgagat
     1741 tggctgtagg ctcatcggca aacaatattt ttgggtcatg cgccacggct ctcgtaatag
     1801 aaactcgctg ttgttcacct cctgaaagtt ggcttggtaa attatcgact cgatgggcaa
     1861 gacccatttt tgcgagaacg tcttttgatt tcttatttgc atcacgcctt gagaacccct
     1921 gcattaaaag cggaagagcg acattttcaa gtgctgttaa atccggcaag agcgcgtagt
     1981 cctgaaaaac ataaccgaac tgaataagtc gaaatttcat tttctcatca ggtcccatgt
     2041 tcttggtgtt agttccgttc aatatgacat caccgccggt cggcgaatca agaagactca
     2101 tttggtaaag aaaagtactt ttaccggcgc cagatcggcc cattatggca acaaattgtc
     2161 cttccgtcgc cgaaaaatcg atgcctttca gaacttttgt ctctatatcg ccgtttttga
     2221 atgattttgt tagtttgatt gtttggatca tagattttct tgagttatac gattagaaaa
     2281 tcatgaatcc cgtagtaagt ctcactaaaa tagagaaagt gtaccgagta aacaaaactt
     2341 cgatcattgt aatttatcac tgtatctaaa cttaaagact ttactactgg gatcatgcct
     2401 ttttttatta gtttctcccg agaatcgaat cgagcgtatt ctttctgata atcatccatg
     2461 ccggaatata gccagcgatg atcgtcgaca cgaccaagag tccgactcta aacaatgtct
     2521 gatcaagggg ggccacgaga ataccgtcgc taaatggaaa atttatcgga tggtcggcaa
     2581 ttacaggtac aagaaacgcg tacacaag
//
LOCUS       DATDSU010000116         3275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1944729, whole genome shotgun sequence.
ACCESSION   DATDSU010000116 DATDSU010000000
VERSION     DATDSU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944729"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VI981_04085"
     CDS             complement(<1..488)
                     /locus_tag="VI981_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY4479504.1"
                     /translation="MEASFIFQFIILIISVIIHEVAHGYMALWLGDPTAKYEGRLTLN
                     PVKHIDLWGSIIIPIITTLAGFTFGWAKPVPYNPYNLRNQRWGDALVAIAGPASNIIL
                     ASLFGLVIRLGMHFSFLSAEFISLASLVVLVNIVLALFNLVPIPPLDGSKIFFSIWGP
                     LR"
     gene            664..906
                     /locus_tag="VI981_04090"
     CDS             664..906
                     /locus_tag="VI981_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HEY4479505.1"
                     /translation="MAKVCPIMHIGSRVGGGYSNRTRATQFNPTGRVRKYPNLQKKKI
                     YIPELKKSVTVLISTKAIKTINKNGAYQTLRKAGII"
     gene            complement(974..1825)
                     /locus_tag="VI981_04095"
     CDS             complement(974..1825)
                     /locus_tag="VI981_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4479506.1"
                     /translation="MQKFLKTLPLFLLAGLAATTFFIWRTIPTSEQLLKVDFLNVGQG
                     DAIFIESPTATQVIVDGGPDKKILSELGDIMPFYDKFIDLLVVTNPDKDHFAGFIDVL
                     SFYKVGNVLVPGTKTDTAVYKEFERLLKEKNITPIVARRGQVFDLGGGALFTVLFPDR
                     DVSTLGTNEGSIVARLSYGNNSVLFMGDAPSNIEAYLEDLDGEALRSTILKVGHHGSK
                     TSTSDDFVKMVKPEMALISAGADNKYGHPHKEVTDILTKEKIEILGTYKDGRTVFYSN
                     GLEFTRK"
     gene            1916..2521
                     /locus_tag="VI981_04100"
     CDS             1916..2521
                     /locus_tag="VI981_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEY4479507.1"
                     /translation="MKYEEQKFNIPDLKGITKKNIEEHLKLYAGYVKHTNLILEKIEE
                     YKKDAEKNAYALGEIQRRFSFEFNGMRNHEYYFKSLEGESKVLTITSPLKKAIESHFG
                     TWSLWLAMFKSIALTRGIGWAILYFDPIAQNLVHAWVDEQHLGQLNGCHIVLAIDMWE
                     HSYVADYQPSGKQQYVEDFFTNLNWTTIEENYNNASTYIRN"
     gene            complement(2659..2973)
                     /locus_tag="VI981_04105"
     CDS             complement(2659..2973)
                     /locus_tag="VI981_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479508.1"
                     /translation="MRLSRARKKRPLVSIVTFTNRPYPALRIIAIEACKTRGVKSIAV
                     KTKDGRRHVAVFRWFTRPRKILPTPAECLPCSSSEGSAFLGWIRVLAVAWPNPALKTG
                     KP"
     gene            3037..>3275
                     /locus_tag="VI981_04110"
     CDS             3037..>3275
                     /locus_tag="VI981_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479509.1"
                     /translation="MFGMSIQGDLHSSFRLAELCSFDDRAAFTSIFCEIGKNFISKTA
                     YRKYTAISNQTGKYEIVREANEKAEKNETTRIIYS"
BASE COUNT         1040 a          727 c          674 g          834 t
ORIGIN      
        1 tagcgcaggg gaccccagat cgagaaaaat atttttgagc cgtcgagtgg aggaatcgga
       61 actaaattaa aaagtgcgag aacaatattg accaagacca caagactcgc gagggaaata
      121 aattctgcgc ttaaaaagct aaaatgcatt ccgagacgaa tgacgagccc aaataaactt
      181 gccaaaataa tatttgaagc cggcccagca atagccacaa gggcatctcc ccagcgttgg
      241 ttgcgtaaat tgtagggatt gtacggcacg ggttttgccc aaccgaaagt gaagcccgca
      301 agcgttgtga taatggggat gatgattgaa ccccaaagat caatatgctt tacgggattg
      361 agcgttagtc ttccttcata ttttgctgtc ggatccccta accaaagagc catatatcca
      421 tgagcaacct catggataat cacagaaatg atgagaatga tgaattggaa aataaatgaa
      481 gcctccattt gcgtatttat tcgattatga taacatcgag aatctaacga gcatagtgag
      541 tatagattct ccgaccccat gcaatatatt tgcgagccaa gaagtggccg caaatataaa
      601 cctaaaggtt tatattgcac ggggtggaga ttttaaaatt cgctccgctc aagaaaatca
      661 ttcatggcca aagtctgccc aatcatgcat atcggttcac gcgttggagg cggctactcc
      721 aaccggactc gtgctaccca gtttaaccca acgggtcgcg ttcggaagta cccaaatctc
      781 caaaaaaaga aaatctacat tcccgaacta aaaaaatcag tgacggtcct catttcaaca
      841 aaagccatca aaacaataaa taagaacggc gcgtatcaga cactccgaaa agctgggata
      901 atttaacatc ttcataaata agttggttaa tatagagaat ccacaatgcg gttttttgtt
      961 tttgtctaga aaattatttc cgtgtaaatt ccaatccatt ggaatagaaa accgtccgcc
     1021 cgtctttgta cgtaccaagt atttcaatct tctctttagt gagtatgtca gttacctctt
     1081 tatgtggatg accgtactta ttgtctgcgc ccgcggaaat tagcgccatt tctggcttaa
     1141 ccattttcac aaaatcgtca ctcgtcgatg ttttagaccc gtgatgaccg accttaagta
     1201 tcgtactcct caatgcttcc ccatcaagat cttccaaata cgcctcaatg ttcgaaggtg
     1261 cgtcacccat aaacaacact gaattattac catagcttaa ccgagcaaca atggatcctt
     1321 cattcgtgcc gagtgtagag acatcgcggt caggaaaaag tacggtaaaa agtgcacctc
     1381 ccccgagatc aaacacttga ccgcgccgag cgacaatcgg tgtgatattt ttttctttaa
     1441 gcaatcgctc gaattcttta tacaccgccg tatccgtttt agtccccggg acgagtacgt
     1501 ttccaacttt gtaaaacgag agcacatcaa taaaaccggc gaagtgatct ttgtctggat
     1561 tcgtgaccac caaaagatcg ataaacttgt cataaaatgg catgatatcg ccgagttcgg
     1621 agagaatttt cttatccggt ccgccgtcaa cgattacttg cgtcgccgta ggactttcga
     1681 taaatatggc atcaccttgg ccgacgttta agaaatccac cttaagaagc tgctcggaag
     1741 tcggaatagt ccgccatatg aagaaagttg ttgcagctag tccggctaac aaaaacaatg
     1801 gcagtgtttt caaaaatttc tgcatgattc aattgtagcg tcattctaag tggtagttaa
     1861 gttatttctc ggggtaacgt ccactgctgt ataatagagt tattaaacaa aatttatgaa
     1921 atacgaggaa caaaaattta acattccgga cttgaagggt ataacaaaga aaaatatcga
     1981 ggagcatttg aaactttacg cgggctatgt aaaacatact aatctcatcc ttgagaaaat
     2041 cgaggaatac aagaaagacg ctgaaaaaaa tgcgtatgca cttggtgaga tacagaggcg
     2101 tttcagtttt gaattcaatg gcatgcgaaa ccatgaatac tatttcaaaa gcctcgaagg
     2161 agaatcaaaa gtcctcacaa tcacaagccc tctcaaaaaa gcaattgagt cacattttgg
     2221 tacatggagc ctatggctcg ccatgtttaa atcgattgca ctgacacgcg gcatcggttg
     2281 ggccatactt tatttcgatc ctattgccca gaatttagtg cacgcatggg ttgacgaaca
     2341 gcatcttgga caactaaacg gttgccatat cgtgcttgcc atcgatatgt gggaacactc
     2401 atatgtcgcc gactatcaac caagcggaaa acaacaatat gttgaagatt tcttcaccaa
     2461 cctaaattgg acgacaattg aagaaaacta caataacgcg agtacataca taagaaatta
     2521 atggactacg tgagtcgctc gtcacacttg gagacttagt ctccaagtgt gaaaaagggc
     2581 tatttgtgtc aaaacccgac ttggagacta agtctccaag tcgatccatg gtcaaatata
     2641 aaaaaaccgc agacgctact acggtttgcc agttttgagt gccggattag gccacgccac
     2701 ggcaagaaca cgtatccatc cgagaaaagc actcccctca ctcgatgaac atggaaggca
     2761 ttcagctgga gtcggtaaaa tctttctcgg ccgcgtgaac cagcgaaata cggcaacatg
     2821 acgacgaccg tctttggtct tcacagctat agatttcact cccctcgtct tgcaagcttc
     2881 aatagcaata atacgaagtg ctggataagg ccggtttgtg aacgtaacaa tgcttacgag
     2941 aggacgcttc ttccgagccc gactcaacct catgagttca tcgcgaatcc ccgttccttc
     3001 ataatacttg acccgataag gtggtaacct ttcttcatgt ttggcatgtc tattcaagga
     3061 gatcttcatt cttcattcag actagcagaa ttatgttctt ttgacgacag agccgctttc
     3121 acgtctattt tctgcgaaat cggaaaaaat tttattagca aaactgcgta tagaaaatac
     3181 accgccataa gcaaccagac gggaaaatat gaaattgtga gagaggcaaa tgaaaaagca
     3241 gaaaaaaacg aaacgacacg aatcatatat tcaag
//
LOCUS       DATDSU010000117         5357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2282448, whole genome shotgun sequence.
ACCESSION   DATDSU010000117 DATDSU010000000
VERSION     DATDSU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282448"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="VI981_04115"
     CDS             complement(<1..2054)
                     /locus_tag="VI981_04115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY4479510.1"
                     /translation="MTPQSPTEEVFRLQDQQKKALKKLGIQTVRDLLHHFHTRYGDTS
                     EIKNINSLVAGESAVIFGKISGLKASKAFRTKITMAEGRITDDTGTIKALWFNQPYIA
                     KMIGDESLVRVEGKISRRRKTDELYFSNPKIEKVEKLPTGVGDSLFGKNGETHSLYPV
                     YPESRGVTSNWFFHAIQKVLVSGALEFVEDPIPKIVLEKYHLPTLKTALIWIHAPKNE
                     NDALSARKRFAFEEVFYIQLERQKERLLLKEQPTHFIQITPDKIKQFLSRFPFSPTSA
                     QRKAIDVILQDLKEGVAMSRLLEGDVGSGKTLVAAATAYGVIETPPKDNKFGRLQVAY
                     MAPTEILARQHFESFIQFFKHLPVNIALITGTECLKFPSKINPNSSTHISRNQLLKWV
                     KGGEISILVGTHALIQKSVEFKHLGYVVIDEQHRFGVNQRKALVRKHKTIPHLLSMTA
                     TPIPRTLALTIFGDLDLTLLDEMPAGRKLIITEVVLPGKRDDVYEKARAELAAGRQAY
                     VICPRIDEPDPSKEMALNVKSAKEEAKRLKESVFPEYEIAVMHGKLKSGDKDTIMADF
                     KTGKIHILVSTSVVEVGVNVPNATVIIVEGAERFGLAQLHQLRGRVLRSTHQAFCYIF
                     ADVKSQKTLDRLSALTKAKNGFELAEYDLQFRGAGELGGRKQWGITDIAMEAIKNIKM
                     VEA"
     gene            2141..2587
                     /locus_tag="VI981_04120"
     CDS             2141..2587
                     /locus_tag="VI981_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479511.1"
                     /translation="MKKIVILEDDHLLLDALCDAFPILVSPELSVTPFLSESAIVQAL
                     PRIAVKPPGLFIFDVMVRFDDFGRTQHPDGRFHAGLRVMRHVRAKQRLASVPIIFHSC
                     LELADLQRAGLERDEATGDILYIAKQTGSKVLFDEVRRLLCEHIAA"
     gene            2655..3002
                     /locus_tag="VI981_04125"
     CDS             2655..3002
                     /locus_tag="VI981_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479512.1"
                     /translation="MNTTIPNFDVALLLNGLQASIRCREQFQSMELKVLREVEMRELS
                     ILVAARYLLNSAERQSSLDSRILTGAVSALERALQCVLIDVHRQELERLLGELKAVER
                     KLKHAADEEAMAV"
     gene            3136..3402
                     /locus_tag="VI981_04130"
     CDS             3136..3402
                     /locus_tag="VI981_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479513.1"
                     /translation="MKVSIAKKKAVWSAETDIVDSARSLLATCEAVKSWQFVSAGFVS
                     RTRGILQDAIGRVGLGDDRKHLEKMLARFEELVSDADEECTFVA"
     gene            complement(3795..4682)
                     /locus_tag="VI981_04135"
     CDS             complement(3795..4682)
                     /locus_tag="VI981_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY4479514.1"
                     /translation="MNINSNRGFAPIIIVIIALLVVGGAGASIYSKNKSKKAAEKAKI
                     ALEEEKKKTDNAMMAKTFQFDLAEQNKSGLTGKVVVNEENGKAKVIVNITGKPSGVPM
                     PAHIHLSSCATIGAVKYPLMSVENGASQTTLTVSLSELLSSLPLSVNVHKSAEEPNIY
                     VACGDIPADSIQSKSDEMMENKDNMMGSDKSKDSADMGPGMMNGVVVVEYTESGFSPK
                     EVTINKGQTVKFINKTSSTFSVASNPHPTHTKLPALDQWKDPKFKSQTTYEFTFTQAG
                     KFDYHNHMKESDGGAVIVK"
     gene            complement(4776..>5357)
                     /locus_tag="VI981_04140"
     CDS             complement(4776..>5357)
                     /locus_tag="VI981_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY4479515.1"
                     /translation="CGGNRNTSEFWLMDPIDGTAHYIRGLPFCTTMIALVQDGKINFS
                     AIYDFVNDHMFFAERGKGAFRDETRLHVSTRSLQESYLCWETHLDNPENMKIFLELRK
                     KVILCNTITAGWECAMIAEGKYDGRITFDPHGKDYDFSPGSLLIEESGGVCKNLYSES
                     YDYRNTSFIAANPKVYEELTSGFDALFPIRAQK"
BASE COUNT         1321 a         1198 c         1250 g         1588 t
ORIGIN      
        1 gcttcgacca tttttatatt ttttattgct tccatggcga tgtctgttat gccccattgt
       61 tttctgccgc cgagttctcc cgcgccacga aattgcagat catattcggc gagctcgaaa
      121 ccattttttg cttttgtgag cgccgacagt cggtcgagcg ttttctggct ttttacatcc
      181 gcaaaaatat agcaaaacgc ttgatgagtg cttctcaata cacgaccgcg aagctgatgg
      241 agctgggcta ggccgaagcg ttccgctcct tcaacaataa tgaccgtagc attcggcacg
      301 ttgacaccga cctcgacgac agacgttgaa acgagaatgt gtattttgcc agttttgaaa
      361 tcagccatga tagtgtcttt gtcgcctgat tttaacttgc cgtgcattac ggcaatttcg
      421 tattcgggga agacgctttc ctttagccgc ttggcttctt ctttagcgga cttcacgttg
      481 agcgccattt cttttgaagg gtcaggctcg tctatgcgag ggcatatgac gtatgcttgc
      541 cggccggccg caagttcggc gcgcgctttt tcgtacacgt catcgcgttt acccggcaaa
      601 acaacttccg taatgatcag cttgcggcca gccggcatct cgtcgagcag tgtcaggtca
      661 aggtcgccga agatggtaag tgcgagcgta cgaggtatcg gcgtcgcggt catacttaaa
      721 aggtggggaa tcgttttatg ttttcgaaca agagcttttc tttgatttac cccgaagcgg
      781 tgctgttcgt cgattacaac gtatccgaga tgcttgaatt cgacactttt ttggatgagc
      841 gcatgagttc caactagaat tgaaatctcg cctcctttta cccatttgag aagttgattc
      901 cgtgaaatat gtgttgaact gttcggattt atttttgacg gaaacttaag gcattccgtt
      961 ccggtaatga gcgcgatatt tacggggagg tgtttgaaga attgaatgaa tgattcaaaa
     1021 tgctgtcttg ccaagatttc cgtaggggcc atgtaagcca cctgaagtct tccgaattta
     1081 ttatctttgg gcggtgtttc aatcacgcca tatgctgttg cggccgcaac tagcgttttt
     1141 ccggagccga cgtcaccttc gagcagtcgt gacatcgcaa caccctcctt taaatcttgc
     1201 aggattacgt caatcgcttt ccgttgagcg cttgtaggcg aaaaagggaa gcgactcaaa
     1261 aattgcttta ttttgtctgg agtgatttga ataaaatgag tgggctgctc tttgagcagt
     1321 aatctctcct tttgacgttc aagctggata tagaaaactt cttcaaatgc gaagcgtttc
     1381 cgcgccgaga gtgcatcgtt ttcatttttt ggcgcgtgaa tccaaatcag tgcggtttta
     1441 agtgtcggca aatggtattt ttcaagcaca atttttggaa tcgggtcttc aacaaattca
     1501 agagcaccac ttacaagcac tttttgtatt gcatgaaaaa accagttcga agtaactccg
     1561 cggctttccg gatagacagg gtaaagtgaa tgtgtttcac catttttgcc aaaaagcgaa
     1621 tcaccaactc ctgtcggtag tttttccact ttttcgattt ttggatttga aaaatataac
     1681 tcgtcagttt tgcgccgcct tgatatctta ccctcaacac gcacaagcga ttcgtcgccg
     1741 atcattttcg caatgtaagg ttggttgaac cacaacgctt ttatggtgcc agtgtcgtca
     1801 gtgattctac cttcagccat ggttattttt gtgcggaagg ctttactcgc tttaaggccg
     1861 gatatttttc caaaaataac cgcgctttca ccagcaacaa gagaattaat attttttatt
     1921 tccgaagtgt ctccatatcg agtgtgaaag tgatgtagga gatctcgaac agtttgtatg
     1981 cctagcttct ttagcgcttt tttttgttgg tcttgcagtc tgaatacttc ttcagttggc
     2041 gattgggggg tcataggggc tattctacat gagcatgagt ttggttgaca aaggtggtgg
     2101 caatagtagg ctggagcgag aaaatcgcaa ttcctctcat atgaaaaaaa tcgttattct
     2161 tgaagatgat catttgttgc tcgacgctct ctgcgatgcc tttccgattc tcgtttcgcc
     2221 tgaactatcc gtcacaccgt ttctgtccga aagcgccatt gttcaagcgc tcccgcgcat
     2281 tgccgtcaaa ccgccgggcc tttttatttt tgacgtcatg gttcgtttcg atgattttgg
     2341 tcggacgcaa catcctgatg gccgctttca cgctggctta cgcgtgatgc gacacgtcag
     2401 agcgaagcag cgactggcga gtgtgcccat catttttcac tcgtgtcttg agctcgctga
     2461 tcttcaaaga gctggactag agcgcgacga ggcaacggga gatatcttgt acattgccaa
     2521 acaaaccgga agcaaagtgc tctttgacga ggtcaggaga cttctttgcg aacatatcgc
     2581 tgcgtagcgc ttattgctgg gcgctttttt cttgacaaaa cacgattttg acgtactctt
     2641 aaactagaaa acgaatgaat acgacaattc cgaatttcga cgtagcatta ctcctgaacg
     2701 gtctccaggc cagcatcaga tgcagagaac aatttcaaag catggaactc aaggttctcc
     2761 gggaagtgga aatgcgcgag ttatcaattc tcgtcgcggc gaggtacctt ctcaactctg
     2821 ccgaacggca aagttcgctg gattcgcgaa ttctcaccgg tgccgttagt gcactcgaac
     2881 gggcgcttca gtgcgttctg attgatgttc acaggcaaga actcgaacgg ctgcttggcg
     2941 aactgaaagc tgttgaacga aaactgaaac acgcggcaga cgaagaagct atggcggtat
     3001 aggtgacctg gagtcgcctt ttttaattta aaacaattta cagcagaagc tgcataacat
     3061 tttcaaaaca ccgagcgaac cccgctcttt ttttgttgaa aaggtgtaaa atagatgagg
     3121 ccaaaatttt tctgtatgaa agtatcaatc gccaagaaaa aagcggtatg gagtgcggaa
     3181 acggacattg ttgattccgc aagaagtcta ctcgcaacat gcgaagctgt gaaatcctgg
     3241 caattcgtca gcgcaggttt cgtctcgaga actcgtggca tacttcaaga tgcgatcggt
     3301 agggtaggac tcggcgatga tcgaaaacat cttgagaaaa tgctcgcgcg gtttgaagaa
     3361 cttgtatccg acgccgacga agaatgcacg tttgtggcgt agcaggcaga ctgaaaaggt
     3421 ctgctttttt ttgcaaaccc cgttagaagc tcacgtgggg cattcaaccc cgtgttcgct
     3481 aaaatcgaat tgcattcatc ccgttagaaa tcacttggtt atgtataatc acttagtaaa
     3541 aaatgagaaa ataggaggga ctcacttcac ttttcaaaaa ttgggcacgg aaatgcttta
     3601 taattgaatt acgtatggca ccaaatgtat tacaaaatat gaatagtaag tgtattttga
     3661 ataaacagaa gcatttccta tttctaacgg gattcatccc cacggcaagc cgcgtggcgt
     3721 tctgcttcta acggggtaaa caaaagccgc ggccttctgg ccgcggcttg cttctaattt
     3781 tttgagtcga cgggttattt aacaatcacc gctccgccat ctgactcttt catatggtta
     3841 tggtagtcga attttcccgc ttgggtgaag gtaaactcat atgtcgtttg gctcttgaat
     3901 ttcgggtctt tccactgatc aagcgcaggg agtttagtgt gtgtagggtg cggatttgat
     3961 gcgaccgaga aagtgctact tgtcttgttt atgaatttta cggtttggcc cttattaatt
     4021 gttacttctt ttggtgaaaa gccgctttca gtgtattcaa caaccactac gccattcatc
     4081 ataccaggcc ccatatctgc gctgtcttta ctcttgtcgc ttcccatcat attgtctttg
     4141 ttttccatca tctcgtcgct cttgctctgt atcgaatctg ccggtatatc tccacaggca
     4201 acatagatgt ttggctcctc tgccgacttg tggacgttga ctgaaagggg aagtgaagag
     4261 agaagttcgg aaagtgaaac agtgagtgtt gtttgtgaag ccccattttc aacagacata
     4321 agcggatact ttaccgcacc gatcgttgcg caacttgaaa ggtggatgtg ggcgggcatt
     4381 ggaactccag agggtttgcc cgtaatgtta acgatcactt tagcttttcc attttcctca
     4441 ttgaccacga cttttccggt caggcctgat ttattttgct cagcgagatc aaactgaaaa
     4501 gttttcgcca tcatggcgtt atccgttttc ttcttttctt cctcgagagc tattttagcc
     4561 ttttcggccg ccttttttga tttattcttt gaataaatgc ttgcgcctgc tccgcccaca
     4621 acaagaaggg caataattac aataataatc ggcgcaaaac cgcgatttga attgatattc
     4681 atgagtcaaa attaatttgt attgataaca ctctacatta taaaggctcg caatgagagc
     4741 gcaagtttcc caccgtttaa tgctgttgtg tggagctatt tttgcgctcg tatcggaaaa
     4801 agagcgtcaa atccactcgt gagctcctca tagacttttg ggttggcggc gatgaaacta
     4861 gtgtttcggt agtcatagga ttcagaatag agatttttac atacgccgcc tgactcctcg
     4921 atgaggagcg aacccggcga aaaatcataa tccttcccat gcggatcaaa tgtaattcga
     4981 ccgtcatatt ttccttcagc aatcatggcg cactcccacc ctgcggttat cgtattacac
     5041 aatattactt tcttcctcaa ttctaaaaat attttcatat tttcaggatt atcaagatgc
     5101 gtttcccagc agagatatga ctcctgcaag gaacgggtgc tgacatgaag gcgcgtttcg
     5161 tcgcgaaacg cgcctttacc gcgctcggca aaaaacatgt gatcgtttac aaaatcatat
     5221 atcgcggaaa aattaatttt tccatcctgt acgagcgcga tcatggttgt gcaaaaggga
     5281 agtcctcgaa tgtagtgggc tgtgccgtcg atcgggtcca tgagccaaaa ttcgcttgtg
     5341 ttgcggtttc cgccgca
//
LOCUS       DATDSU010000118         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1948775, whole genome shotgun sequence.
ACCESSION   DATDSU010000118 DATDSU010000000
VERSION     DATDSU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948775"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..701)
                     /locus_tag="VI981_04145"
     CDS             complement(153..701)
                     /locus_tag="VI981_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY4479516.1"
                     /translation="MNENKHKWIGITGTWRRTNNEVEQDVRSAVREIITRGDGVVVGG
                     ALGVDYFALNEAIRVNPDAARIKVVLPSSLKDYIAHLQMWASGYDTGDPAITSEEAER
                     LANQLRGLEKINQGSIVESSTETGDKINKDVYFTRNSIVVELSNEVIAFQVNDSAGTQ
                     DTINKARGAGKNVIVHSYSITK"
     gene            complement(698..1540)
                     /locus_tag="VI981_04150"
     CDS             complement(698..1540)
                     /locus_tag="VI981_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4479517.1"
                     /translation="MTGRLLEYEFWKNHNAYYVGFFMDALAKKDRLEKRKYFAKEILE
                     TPTRPIRWLDVGTGDGSKILDTINFIKERERGAIIELITIEPSEYIIAELKEKLSKVD
                     GVSLKVINEKFALEKLKPNTFDCITFFHVAYYLADEETGFEKLYLRAYESLKLDGILL
                     VQAVNESGDFQALGHPSYSIWAQGDHTFDLLKRISPKTHRENFPTHFDVTDFLTQKNS
                     TPEFDDKLLNLFRFTTQVDSAVLTKEGGEKYISHMRELAKQNGNTFYLDFSDVVVSLH
                     KSNK"
     gene            1988..2680
                     /locus_tag="VI981_04155"
     CDS             1988..2680
                     /locus_tag="VI981_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479518.1"
                     /translation="MKSKWFNLREEAIAMRQKSASIKDIEDKLKIPRSTLSGWFRTIK
                     LTRGQKDLIYKRWRQRLRATRKLAIAWHNNQKLIRLKEAELQASESLKNIDIHDPSVI
                     NLALSMLYLGEGFKKKAFGMGNSDSMILKLFVSLLLKTYLIPIDKISCELHLRADQNP
                     NKIKKYWSKELGLPISRFRWVSLDQRTVGSPTYPHYKGVCIVRCGSIAIQRKLLYTSK
                     IFCEKVIDRMRA"
     gene            2671..2745
                     /locus_tag="VI981_04160"
     tRNA            2671..2745
                     /locus_tag="VI981_04160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2703..2705,aa:Val,seq:gac)
BASE COUNT          910 a          810 c          748 g          935 t
ORIGIN      
        1 tttggcgggg gtgaaatttc ttttgaaaaa ggaaagggtg tttttctttc ggggttctgc
       61 cctccaagta ttcgggcagt gtggcggggc gaaacgcaaa tgccggagga aattttttgc
      121 gcgcattttt caagaacaaa aaagtgccgt cactactttg tgattgaata cgaatggact
      181 atcacatttt tccctgcgcc tctcgcctta ttaattgtgt cttgtgtgcc agcactatca
      241 ttaacttgaa aagcaataac ctcgttcgaa agttcgacaa caattgaatt gcgagtaaaa
      301 taaacatcct tgtttatttt gtcgcctgtt tcagtcgaac tttcgacaat tgatccttga
      361 tttatttttt caagtccgcg aagttgattg gcaagtcgtt ctgcctcctc cgaagttatt
      421 gccgggtcgc ctgtatcata tccacttgcc cacatttgca gatgagcaat ataatctttc
      481 aatgaggaag gcagaacgac tttgatacgc gcggcgtcgg gattaacgcg tatcgcctcg
      541 ttaagtgcaa aataatcgac acccaacgca cctccgacaa caacgccgtc accgcgcgtt
      601 ataatttcgc ggactgcgga acgaacatct tgttctacct cattgttcgt tctacgccat
      661 gttccggtaa tccctatcca tttatgttta ttttcgttca tttgtttgat ttgtgcagag
      721 aaacgacgac atcactaaaa tccaaataaa aagtgttacc attttgcttc gcaagttcgc
      781 gcatatgcga gatatatttt tcaccccctt cttttgtcag cacggccgaa tccacttgtg
      841 ttgtaaaacg aaaaagattg agtagtttat cgtcaaattc tggtgtcgaa tttttttgtg
      901 tgagaaaatc tgtaacgtca aagtgcgttg ggaaattctc tcgatgtgtt tttggtgaaa
      961 ttcttttcaa taaatcaaag gtatgatcgc cctgtgccca aatcgaataa gacggatgcc
     1021 caagtgcttg aaagtcgcca ctctcattaa ccgcttgcac caaaagtatg ccgtcgagtt
     1081 tcaaggattc atacgctcgt agataaagct tctcaaaccc cgtttcttcg tcagctagat
     1141 agtaagcgac gtgaaagaaa gtaatgcaat cgaaagtatt aggcttcaat ttttccaaag
     1201 caaatttttc atttatcact ttgagtgaaa cgccatcaac ttttgacaat ttttctttaa
     1261 gctccgcgat tatatattca ctcggctcaa ttgtgataag ttctattatc gcgccgcgct
     1321 cgcgttcttt gataaaatta atcgtgtcca aaatctttga accatctcca gttccaacat
     1381 caagccatcg aattggtctc gttggcgttt ctaaaatttc ttttgcaaaa tactttcgtt
     1441 tttccaatcg gtctttcttc gcaagcgcat ccataaaaaa accgacataa taggcgttgt
     1501 gatttttcca aaattcgtat tcaagaagtc gtcctgtcat aaaaaattag gtattttacc
     1561 ttgcttcgat tttagcataa atctttattt ttttgaattg cttggtgcgc gcaaaaaatt
     1621 tcctccggca tttgcgtttc gccccgccac actgcccgaa tacttggagg gcagaacccc
     1681 gaaagaaaaa caccctttcc tttttcaaaa taaatttcac ccccgccaaa gcagaaatgc
     1741 aaggagtgtt ttttctttcg gggttgcaga gtgaagcgag gcggtggcgg ggctttcgtg
     1801 gagcgtacag attagcttcg cttaaccacg cacgcggagc gtgcgaattc attacctcac
     1861 tttgttttga aaataggttc taacttggta caatagagac acaaataaaa caaaggacac
     1921 ccgttttggg tgtcctttgt tgataatacc cctatcatat ccgtactaag gagagtataa
     1981 tgtatacatg aaatctaagt ggttcaatct aagggaagag gctatcgcca tgcgccaaaa
     2041 gagcgcatca atcaaagata ttgaggataa acttaaaatc ccccgctcta cgctgagcgg
     2101 atggtttaga acaataaagc taaccagagg ccaaaaggat cttatttata aaagatggcg
     2161 acagcgactg agggcaacca ggaaacttgc aatagcatgg cataacaatc aaaaattaat
     2221 ccgactcaaa gaagcagaac tgcaagcgtc tgaatcactt aagaatattg acatacatga
     2281 cccttcggtt ataaacctcg ctttgtctat gctttatctt ggagaaggat ttaaaaagaa
     2341 agccttcggc atgggaaatt ctgattcaat gatactcaag ttgtttgtgt ctttactttt
     2401 gaagacatac cttatcccta tagacaaaat atcttgtgaa ttacatttgc gcgcagacca
     2461 aaatcctaac aagattaaaa agtattggtc taaagaactt ggtctaccga taagtcgctt
     2521 tcggtgggtc agtctcgacc aaagaacggt tgggtctccc acttatcctc attacaaggg
     2581 ggtttgcatt gtgagatgcg gctcaattgc catacaaagg aaattactgt acactagtaa
     2641 aatcttttgc gagaaagtta tcgataggat gcgcgcttag ctcaatggta gagcgtctcg
     2701 ttgacgtcga gaaggttgaa ggttcgatcc cttcagcgcg caccagacag ggatcgcctt
     2761 ggccagacgg tcgaatcgcc ctaaagagaa gccctaactc agaggtttgt atttgctggt
     2821 agacgtagcc agccggtaag aggaactgcc caaacgcgtt ccggtgcacg ggcggcaggc
     2881 atcgccagcg cgccgaaaga tcgccgagcg ccaccaccag gtcgctggct tcggtccggc
     2941 tcagcggccg ttctgcccca gagaattcgt cagcttgaca ctccaagatc tgacgtcgaa
     3001 gtcggtcgcg ggcagccgta aactcgtcgt ctgacagaag cttgtctgct cgcagttcca
     3061 ccaactcgcc caaccgtcgt tggagtccct tcagccactc ccggcgcttc cggagcgccg
     3121 cttgcgccag agcctcctgc gatcccaaag ctctgacaat gcgcgtgact gtcgtttccg
     3181 cgacgtcggc tggcaatgcc agcgctgaga ggaatccatc aaactcctga tggatcgccg
     3241 acgccggcac acttttcctg gcccgtgggc atccgcgacg ctgacaggag taatacgcgt
     3301 atcggcctcc atgccccttg ctccacgcgg ccgtaaatgc ccaacggcat gacggacagc
     3361 ggaccagccc ccggacgggg aactcttccc gaaaacgcgt gtg
//
LOCUS       DATDSU010000119         5455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_289402, whole genome shotgun sequence.
ACCESSION   DATDSU010000119 DATDSU010000000
VERSION     DATDSU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289402"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VI981_04165"
     CDS             complement(<1..877)
                     /locus_tag="VI981_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY4479519.1"
                     /translation="MSFSKTHSAQTSLLKAHIVDIEVDLAGGLHSFSIVGLPDKAVEE
                     ARDRISAAIKNSRFNFKSPKNRNQKVVVSLAPAELKKEGSAFDLGIALAYLLASREIK
                     FSPEKKIFLGELSLDGALRRTDGILPLVRHAKEKGFTEVYVPLENAEEAALIDGITIF
                     AARTLHEIINHLHEKDMIGKDEDIIPHKHKPLSPVPKTEIIFEKENSEIDFDDVKGQE
                     TAKRGLEIAAAGGHNIAMYGPPGTGKTMLAKAFAAILPSLSFDDILETTAIHSVAGAL
                     SGALVTRPPFRSPHHT"
     gene            complement(886..1248)
                     /locus_tag="VI981_04170"
     CDS             complement(886..1248)
                     /locus_tag="VI981_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY4479520.1"
                     /translation="MTQNTKHFKEMLESELATLEKELSSVARRNPSNPNDWEPVPSKM
                     DIDPADENIVADSIEEFEENAGVTKQLEIQYNDIKRALKQIEDGTYGTCSVCKKEIET
                     DRLNANPAAFTCIAHMNS"
     gene            complement(1272..2669)
                     /locus_tag="VI981_04175"
     CDS             complement(1272..2669)
                     /locus_tag="VI981_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479521.1"
                     /translation="MAPNENKKIDTDAPLSGFHGMKTDQDPLYFEKISQKETEMNASR
                     SQNPLAENGKDEIQELPKLDWSFHGPNKDGAIAESETKGASGVKPDLKNIHTLEGDKE
                     LARPNTIDELPPLPVFEDEEKKVVHSPGFLGMPTEEELPETVASQTSPENVAEHELQA
                     SSETIETNDEDESKSIADLIRAERDTENTTKLATVHTLGDDVSNALKKGASQTSMAIA
                     ESQHRNYEPKETSIETKTILTVVSIVLILMGIGILLYLLFGRSKPPLPPLPPPALVKS
                     DFERTADTSSLESFFYRHKNELRRQIPNNQIVEIKLQTKEIKDDVTYERPLATKEFVT
                     LIKADVPPSLLRTFMDDFFLGIHSFNEINYPFLIIKVSNYDTAYAGMLLWEKNMENSL
                     ADLFEYKKGVIEIKERVFEDGIIENKDVRFLLDQNSSTKLLYSFTDPTTLVITTNEKT
                     LREVGERFRTSKLVR"
     gene            complement(2807..4918)
                     /locus_tag="VI981_04180"
     CDS             complement(2807..4918)
                     /locus_tag="VI981_04180"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY4479522.1"
                     /translation="MQKKEFSVDLGGKKLTVTFSDLAERASGSVLMQYGNSIALVTAV
                     MSKKEKSGAGFFPLTVEYEEKFYASGQILGSRFVRREGRPSDEAILSGRVVDRTIRPL
                     FDQWIRHEVQVIVTILSIDEDDPDVLGVIGASLALGVSDIPWNGPVSAVRIGKMKDTD
                     AFIINPTYKMRENPEAEFELFACGKDGAINMIELGGSEVPEKTVMQALAYASEEIEKL
                     QTFQKKVIKEMSREKRKVPKPKTPAELIELFNKKIKLRMDSVIFAGAGSSRLDALKDE
                     WIALYSESYPESDTSLADHYFEDEVNDFLHREVLNNNRRPDGRSMDEIRNLFAQAGGI
                     SPVLHGSGIFYRGTTHILSALTLGGPGDSQLIEGMEEQTKKRFIHHYNFPPFSVGETG
                     RVGGMNRRMIGHGALAEKALEPVIPPKETFPYTIRIVSEALSSNGSTSMGSVCGSTLA
                     LMDGGVPIKEPVAGIASGLIMESPSKYKVLTDIQGPEDHHGDMDLKVAGTKNGITAIQ
                     MDVKVSSIPLAILEEALEKARLARLKILEVILQAIRAPREDISPKAPKILTIKVKVDQ
                     IGLVIGPGGKTVNGIREKTSVDEIDIEEDGTIFITGKNGSAEKARQIIYDLTREYMPG
                     EKFTGEVTRLMEFGAFVKIGANAEGLVHISEIAPFRIERVSAYLKEGDKVPVVIKEID
                     EKKRINLSIKAADPSFIKKKA"
     gene            5211..5396
                     /locus_tag="VI981_04185"
     CDS             5211..5396
                     /locus_tag="VI981_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479523.1"
                     /translation="MKNNMEVQPEVRKHNKHNGGNKDAAQPEPDVIALLEESEFSASS
                     FGHLMRRTPPIESALHD"
BASE COUNT         1313 a         1241 c         1221 g         1680 t
ORIGIN      
        1 aagtatggtg gggtgaacga aaaggcggac gagtgaccag tgcgccagag agagcgcccg
       61 ccacagagtg aatggctgtt gtttcgagga tgtcgtcaaa actgagcgac ggcaaaatag
      121 cggcaaaagc tttagcgagc atggttttgc ccgtacctgg cggcccatac atggctatgt
      181 tgtggcctcc ggcggcggct atttcaagac cacgttttgc agtctcctgc ccttttacgt
      241 cgtcaaagtc tatttcagaa ttctcttttt caaaaatgat ttcagtcttt ggcacaggcg
      301 acaaaggctt gtgtttatgc gggataatat cttcgtcttt gccgatcata tctttttcgt
      361 ggagatgatt gataatctcg tgaagagttc tggcggcaaa aattgtgatt ccgtcgatga
      421 gcgccgcttc ctcggcgttt tcgagcggca cgtacacttc ggtgaacccc ttttcttttg
      481 catgtcggac gagaggaaga atgccgtcag tacgccgcag ggccccgtcg agcgaaagtt
      541 ctcctaaaaa gattttcttt tccggactaa attttatttc ccttgaagct aaaagatatg
      601 caagggcaat acccaaatca aatgccgatc cttctttttt aagctcagcc ggagccagtg
      661 aaaccacaac tttttgattc ctgttttttg gagacttaaa attaaagcga gaatttttta
      721 ttgccgccga gatacggtca cgcgcttcct caactgcctt gtctggcaaa ccgacaattg
      781 aaaatgaatg aagacctccc gcgaggtcca cctcaatatc cacgatgtga gctttgagaa
      841 gcgaagtctg ggcgctgtgg gtttttgaaa agctcatgga tcaatctaac tattcatgtg
      901 tgcaatgcaa gtgaaagcgg ccggattggc attgaggcga tctgtttcga tttctttttt
      961 gcaaacggag catgtgccgt atgttccgtc ctctatttgt tttagtgctc tctttatatc
     1021 gttatattgg atttccagtt gttttgtaac gcctgcattt tcttcaaatt cttcaatact
     1081 gtccgccact atattctcat ctgcaggatc aatgtccatt tttgatggca caggttccca
     1141 gtcattgggg tttgatggat tgcgcctcgc aacactgctc aattcttttt cgagcgtggc
     1201 gagttcagat tcaagcattt ctttgaaatg ttttgtattt tgtgtcatgt gaaaatgata
     1261 acacggccta tttaccgcac caacttagac gttcgaaaac gttccccgac ctcccgtaat
     1321 gttttttcat tagtggtgat gacaagtgtc gtcgggtcag taaatgaata gagcagtttt
     1381 gtcgaactgt tttgatcgag caaaaaacgg acgtctttgt tttcaatgat accgtcttca
     1441 aagactcttt ctttgatttc gatgactccc tttttatact cgaaaagatc agcaagtgag
     1501 ttttccatgt ttttttccca caaaagcatt ccagcatagg cggtatcata gttcgaaacc
     1561 ttgatgatca ggaacggata attgatttcg ttgaaagagt gtataccgag aaaaaaatca
     1621 tccataaatg ttcgcagtaa tgagggagga acgtcggctt ttataagcgt cacgaattct
     1681 tttgtagcga gcggacgttc atacgtcacg tcatctttga tttctttggt ctgtaatttt
     1741 atttcaacaa tctgattgtt cggtatttga cggcgcagtt catttttatg tcgatagaaa
     1801 aatgattcaa gtgaacttgt gtctgcggtg cgttcaaaat cgcttttgac gagagcgggc
     1861 ggaggcaacg gagggagggg tggtttgctc cttccgaata aaagatacag gagaatgcca
     1921 attcccataa gtatgagcac aatactcaca acggtcagta ttgttttcgt ttcgatcgaa
     1981 gtttctttcg gttcgtagtt gcggtgctga ctttccgcaa tggccatgct tgtctgcgag
     2041 gcccccttct tcaatgcatt actgacatca tctccaaggg tgtgtacagt tgcgagtttc
     2101 gtcgtgttct ctgtatccct ttcagccctg atgagatcgg cgatgctttt ggattcatcc
     2161 tcgtcgtttg tctctattgt ttccgaactt gcttgaagtt catgctcggc aacgttttcc
     2221 ggagaggttt gagacgcaac ggtttcggga agttcttcct ccgttggcat gcctaaaaat
     2281 ccaggtgagt gaacaacctt tttttcctca tcttcaaata cgggtagagg cggcagttcg
     2341 tcaatcgtat tcggtcgggc gagctctttg tcaccctcta gtgtgtgaat gtttttgaga
     2401 tctggtttta ctccggacgc acctttagtt tctgattcgg ctatggcccc atctttgttt
     2461 ggaccgtgga aactccaatc taacttaggc agttcctgta tttcgtcctt gccattttca
     2521 gccaacgggt tttgagacct agatgcattc atttcggttt ctttttgcga aatcttctcg
     2581 aaataaagtg ggtcttgatc ggttttcatt ccatgaaaac cggataacgg agcgtctgta
     2641 tctatttttt tgttttcgtt gggagccatg ttctgacaaa tgtactacgc tcaacagaac
     2701 tcatcggtgg cgagcgttca acaaacctgc atatgcagga tgaaggtcat tttaccattc
     2761 tcctcggttt cgacctgtgg aggcgcgcgc agagttttcg agtgtcttaa gcctttttct
     2821 tgataaagct cggatctgcc gctttgatgg aaaggttgat gcgttttttc tcgtcgattt
     2881 ctttgatgac tacaggcacc ttatcccctt cttttagata tgcggaaacc ctctctattc
     2941 tgaaaggggc aatttcagaa atatgaacaa gtccctctgc gtttgcccca attttgacaa
     3001 aggctccgaa ttccataagc cgtgtcacct ctcccgtgaa cttctcgcct ggcatatatt
     3061 ctcgtgtaag gtcatagatt atctggcgcg ctttttccgc gctcccgttc ttgccggtta
     3121 tgaaaatagt tccgtcttct tcaatgtcta tttcgtcaac actcgttttt tcccgtattc
     3181 cattgactgt ttttccgcca ggaccgatca caagacctat ttgatccact tttactttta
     3241 tcgtaagaat ttttggggcc tttggagaaa tgtcttctcg aggtgcacgt atagcttgaa
     3301 gtattacctc gagaattttc aatctcgcga gccgtgcctt ttcaagcgct tcttcaagaa
     3361 tggcaagcgg aatgcttgaa accttgacgt ccatttgaat agctgtgatt ccattttttg
     3421 tgccggctac ttttaaatcc atatcaccgt gatggtcctc tggtccttga atatcagtca
     3481 aaactttgta ttttgatgga ctttccataa tgagccccga agcaataccg gcaacgggtt
     3541 ctttgatggg cactccacca tccattagcg caagcgttga accacacact gaacccatgg
     3601 aggttgagcc gtttgatgag agcgcctcag aaacaattct aatggtgtat gggaaagttt
     3661 ccttgggcgg aattacgggt tcaagcgctt tttcagcaag ggcgccgtgt ccaatcattc
     3721 gtcgattcat tccgccgact ctgccggttt cgcccactga aaacggagga aaattataat
     3781 gatgaataaa acgttttttt gtctgctctt ccattccttc gataagttgt gagtcaccgg
     3841 ggccacccaa cgtaagcgcc gagagaatat gcgtagtacc gcgataaaaa ataccagaac
     3901 catgaagcac tggcgaaatt cctcccgctt gcgcgaagag gttccgtatc tcgtccatcg
     3961 atcggccatc cggacggcga ttgttgttga gtacttccct atgtagaaaa tcgttaacct
     4021 cgtcttcaaa gtaatgatcc gctaatgatg tatcggattc gggataagac tcgctgtaga
     4081 gagcgatcca ctcatcttta agggcatcaa gccgtgaact tcctgctccg gcgaagataa
     4141 cgctgtccat tcgcagttta atttttttat tgaaaagttc gataagttct gccggcgttt
     4201 ttggtttagg aacttttctt ttttctcggc tcatttcttt tattactttt ttctggaagg
     4261 tttgaagttt ttcaatttct tcactggcat acgccaaagc ttgcatcaca gttttttccg
     4321 gtacctctga tcccccaagt tcaatcatat ttatggcccc gtcttttcca caggcaaaaa
     4381 gttcaaactc tgcctccggg ttttcgcgca ttttatatgt gggatttatg ataaatgcgt
     4441 cagtatcttt catttttcct atacgtactg cagaaactgg gccattccag ggaatatctg
     4501 atacacctag ggcgagtgac gctccgatga ccccgagtac atcaggatcg tcttcatcga
     4561 ttgagagaat ggtgacaatc acctgaacct cgtgccgaat ccactggtca aacaagggcc
     4621 gtatagttct gtcgacgacg cggccagaga gtatcgcttc atctgatgga cgaccttctc
     4681 ggcgaacaaa gcgtgaacct aagatttgac ccgatgcgta aaatttttct tcatactcaa
     4741 cggtcaacgg aaagaaacca gctccactct tctctttttt cgacatgact gcagtgacga
     4801 gcgcgattga attgccatat tgcataagta ccgaaccact ggcccgctct gctaaatccg
     4861 aaaaggttac tgtcagcttt tttcccccga gatcaactga aaattctttt ttttgcatag
     4921 ttcaaactat gatttgcgtt acggccgcaa aatcaggtga gatctataga ttaaagactt
     4981 gcaacgccta aaggcgtgaa atctgtgtac gtgctccgta gcgaacatat tcgtgtggca
     5041 gagattcaat tcttgtcatg attctcgaat ttcgagaatg caaacaaaaa ttgaagattc
     5101 tgctttgggg tctctctaga tctctttaat gataatgaaa gaagtctaac aaactaggcg
     5161 ttttcaggcg agtttgacaa aataatatat agtatgtaga gtgcatcaag atgaaaaaca
     5221 acatggaagt tcaaccagaa gtgcggaagc acaacaaaca caacggcggc aataaggatg
     5281 cggcacagcc ggagcccgat gttatcgctc tcctcgaaga gtcagaattt tctgcctcga
     5341 gcttcggcca cctcatgcgc cgtacgccgc ctatcgagtc agcgcttcac gattaaggaa
     5401 ccctacggtt cctttttaat ttacaggttc gtcaggccca atgtagtcag ccccg
//
LOCUS       DATDSU010000120         7600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1950095, whole genome shotgun sequence.
ACCESSION   DATDSU010000120 DATDSU010000000
VERSION     DATDSU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950095"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VI981_04190"
     CDS             complement(<1..750)
                     /locus_tag="VI981_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEY4479524.1"
                     /translation="MREIIIAINGLGRIGRAFLKLALKRPEIQIVAVNDLGEITNMAY
                     LLKYDSAYGKNDSDIHVEGGELVVDGKRVKYLCEKDPAKLPWRDLGVDIVVESTGLFT
                     SAEKAKAHLDAGAKRVVITAPLKESETRSDLGSGERSDLGSGKTVLMGINEADLASCE
                     VSSNASCTTNAASPLIAILDEAIGIEKAILNTVHGYTASQALVDGPAKRGWREGRAAA
                     QNIVPSSTGAAIAVTKAFPKLDKLFDGISVRV"
     gene            1058..1807
                     /locus_tag="VI981_04195"
     CDS             1058..1807
                     /locus_tag="VI981_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY4479525.1"
                     /translation="MLSFFNKKTDSEIKMAGKNTLPVTLLTPVEIAADEQRDLDRIND
                     EFKQAFAFLKKYPKSVSIFGSTRFTPENEHFKDARLLASKIAAKGYAIITGGGPGIMQ
                     AANQGAKESGGASVGISIALQKEQETNEYVTDTVTFQYFFTRKTVLSFAAEAYVFFPG
                     GFGTLDEFFEILTLVQTNKIPRVPIVLVGRDYWGALDEFILVNVEKRHRAIRPEERLL
                     YTILDDHAEILKLIESAPVQNWWKNFSVDSS"
     gene            complement(2039..2983)
                     /locus_tag="VI981_04200"
     CDS             complement(2039..2983)
                     /locus_tag="VI981_04200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY4479526.1"
                     /translation="MYERISEVDIERPFKKETDSATKILFLGDIMLGRAVENLQNGHG
                     NDYPFSKVGEFLKKYDVVVANLEGPIMAHHVQTPLFSTRFSFVSSTPKLLKKYNIGIV
                     SLANNHTSDYGLSGLEDTRKSLMAEGIIGVGGSGEDEYSLQKIMINNTSVAILALNSV
                     FHTPDRQKIKNIISTVEKEMTVIVLVHWGDEYIPLSNRRERELAHFLIDEGADLIIGH
                     HPHVVQEVERYNGKIIFYSLGNFIFDQYFSKETQEGIAVEFVIDNEDVSYKIYPFESN
                     KSMPELLVDDRQEWIEEFSKRIRTIERGELIGKIQTQK"
     gene            complement(3027..3512)
                     /locus_tag="VI981_04205"
     CDS             complement(3027..3512)
                     /locus_tag="VI981_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEY4479527.1"
                     /translation="MNQLVLLSITILIMVPGIAGVFIPIIPGIPYMFFIALLYGIADR
                     FQTLSLSNVLFLGLIVLFGLVVDYLSGTLGAHIGGASRRSLLFGLAGAVVGLLLMPPF
                     GGIIGVVVGVAFGEYMHKKKMHEIAKSAVGALVGVLTGMSINFTLALVFFISFVPFAI
                     K"
     gene            complement(3516..4232)
                     /locus_tag="VI981_04210"
     CDS             complement(3516..4232)
                     /locus_tag="VI981_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY4479528.1"
                     /translation="MIPSQNFEWSEANVVEKTNLGERLVGLRLCVPGWVTHTPGQYID
                     VRIKTQKGYNAVKQYSISSPPHDQSVELCVEIFENGEVSPILRDLNIGNKLDVRGPLG
                     VHFVWDGSASEPVVAIAGGTGITPFVSMLRHYEHRALRQDFFLFASAQNQKKLPYISE
                     IMSLAKKATAVTFVPVMTRDSTWDGPKGRIDAVLLRQILKLKNPLVYICGSTSFVEHV
                     VDLVVKFGFSQNNIRTEFFI"
     gene            complement(4263..7151)
                     /locus_tag="VI981_04215"
     CDS             complement(4263..7151)
                     /locus_tag="VI981_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479529.1"
                     /translation="MNGSGRLAQASESTDGGFFLLPARGLISRDFLSEAKIKMDTIKQ
                     SHDFAKKLTALCLIVAFLFATPFVGFAEEQGGETAAEPVVTAPPPTSETPTPETPTSE
                     TPEPESAPDAINPANEGNTETTSTESSGPVIVTGDAEAQGEVTNFVNTNDTTTNGTST
                     SPSVTALSLDGTASTTDATTASTTEATSQETRTGPLEIEVANTGTVGNTMTVEASTGS
                     NTASGTPNSSIDTGSAYGEANVVNVINTNILDSEVFLMFLSNYFSQIGSLDLRFMAST
                     TEDANCGEGCSNPPLSLESYLNSNNIASISNDILVRSGTGGNNILGAGGILTGDAYAA
                     ANVINLANTNIVNSNYMLMVFNNFGDWAGDLVLPSADYFESNFSFGGSGSSQSTPSTA
                     SSSSAFGDVSNTNDALVTTTGTTSASTGGNEVSGTSTGIRTGRALSFASIINRLNSNY
                     TNTDQLFIVFRIAGRWNGNVFGRPNNVEWRDTGNGLEVYGLGSGETNSNSASVNTINT
                     QNNAAITNNVSVYALTGENKIRGNGDSGISTGNAYAASSVLNLANTNVYGRNWILAII
                     NIFGDWTGNLSFGRPDLWIGSRAEVAGELGPGGSARYVYTISNRGDATARSVKLKNSY
                     DPRLITFENQSGWTSEWHLGDLRPGETIERVYLGTVAPTLPIGAFDVVSTATVTANEP
                     DGNPSDNTDSISILARRQNDYARVTWDAFDPIISVVKTNSATTSITASSTVDYMIKLH
                     NEGGVAYDAVLVDKLFDQDGKLINEQSWDLQDFLAGEEITVTYTSVFSEATLPGVYTN
                     EAYVEAMARQRNIHVGNAYTSPKGTSTITVAGSIAVATPTGGGAGGSEVGENVTSSAA
                     STTTPQTTSNIQDSVARTIQLGAALLGGTTEEDSINGTSSGSYLVSVAEANEDLGNNR
                     NSFAAAFFAAQSGEFDFWLLLLALVILLIIMVPKERARKLIGRFSSFF"
     gene            complement(7203..>7600)
                     /locus_tag="VI981_04220"
     CDS             complement(7203..>7600)
                     /locus_tag="VI981_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479530.1"
                     /translation="NGGNTATGGQGGIITSGNALASAHVENNVNYNETEVSSSAEGDV
                     RVRNRNGASVGNLAAVSADTGLNVADGGRAKNKVRGADVSSSSSWGAISAGNGGNDST
                     GGMGGDILTGTVEAHARVVNNINTNITRR"
BASE COUNT         2052 a         1811 c         1599 g         2138 t
ORIGIN      
        1 tacacgcacg gaaatgccgt caaatagctt atcgagtttt ggaaacgctt ttgtgacggc
       61 aattgccgcg ccggtagaag acggcacaat attttgggcc gccgctctcc cctctctcca
      121 gccgcgcttt gccggaccat cgacaagcgc ctgactcgcc gtatagccat gcacagtatt
      181 taatatggct ttttcaattc ctatcgcttc atcaagaatc gcaattaagg gactcgccgc
      241 atttgtagta catgaggcat ttgacgaaac ttcacatgaa gcaagatcag cctcgtttat
      301 tcccataagc acagtttttc ctgatccaag gtccgacctt tctcccgatc caaggtcgga
      361 ccttgtttcg gattccttca gcggagccgt aatgactacc cgcttagccc cggcatccaa
      421 gtgagcttta gctttttccg cggacgtaaa aagtcctgtt gactcaacaa caatatctac
      481 accgagatcc ctccacggca gtttcgccgg atccttttca cataaatatt tgacacgttt
      541 gccatctacg acgagctcac cgccttcgac atgaatatcc gagtcgtttt taccgtatgc
      601 actatcgtat ttgagaagat acgccatatt agtaatctct cctaagtcat taacagcaac
      661 aatctgaatt tccggacgct tcaaggcaag ttttaaaaat gcccgaccaa ttcggccaag
      721 tccattgatt gcaattatta tttcacgcat aagggttatt gtaccaataa ttctaggaat
      781 actttgtgca taaactcaca cgtctccctt aataacataa tgcgataaat gtgcagcccg
      841 tcaatataca ttaaaattac gcggccgcgt aattttaatg tatattacaa agacatgcgc
      901 gcgaaattgc ttttagggga agcattccgc gattgctatt gtgtgatgag gggagtggta
      961 tgcataaact cgtagtcccg agataatcat tttcttattt gagggacgta cgtaattgcc
     1021 taaagtccta atttccctta caatatcaag atatttaatg ctttcttttt tcaacaaaaa
     1081 aacggattcg gagatcaaaa tggctggtaa aaatacgctc ccggtaaccc ttttgacgcc
     1141 agtagaaata gcggcggacg aacagagaga tttagataga attaatgacg aattcaagca
     1201 ggcatttgct tttctcaaaa aataccccaa aagtgtcagt atttttggct cgacgcgatt
     1261 tactcctgaa aatgagcatt tcaaagatgc acgcctctta gcgtcaaaaa tagccgcaaa
     1321 agggtatgcg attataactg gcggcggacc gggtatcatg caagcggcca accaaggagc
     1381 aaaggaaagt ggtggcgcat ctgtcggtat ttcgatagca ctccaaaagg agcaagaaac
     1441 taatgaatac gttacagaca ctgttacttt tcagtatttt tttacgagaa aaaccgtact
     1501 ttcgttcgcg gccgaagcgt atgtcttttt tccgggagga tttgggactc ttgatgaatt
     1561 tttcgagatt ttgacactcg tgcagacgaa taagatccca cgagtgccaa ttgttttagt
     1621 cggcagagac tattggggcg ccctcgatga gtttatattg gtaaatgttg aaaagcgaca
     1681 tagagcaata cgacctgaag agcgattact atacacaatt ctcgatgatc atgctgaaat
     1741 tttgaagctt attgaatccg ctcctgtcca aaattggtgg aaaaatttct cagtcgactc
     1801 atcgtaaacc ccgttagaag ctctcacggg acattaaacc ccgtgttcgc aaaatcaaat
     1861 tgcattcatc ccgtaaaagc agatcctgtt agaagtccta cttatttcca aagtggcact
     1921 tcaagcttta tcctgtttga ctagactaag gtgataataa atcatttggg taaacttcta
     1981 acgggacagg tcgcaatcat cgatcgcggc ttctaacggg gtaaatctcc gacgagaact
     2041 atttttgtgt ctgaattttt ccgattaatt ctccacgttc gatagtgcga atgcgttttg
     2101 aaaattcttc aatccattct tgtctatcgt caactagaag ttctggcata cttttattgc
     2161 tttcgaaagg ataaattttg taactcacgt cttcattgtc aataacaaat tcaactgcaa
     2221 taccctcttg cgtttcttta gaaaagtact gatcaaaaat aaagttaccg agagaataaa
     2281 agataatttt tccgttgtag cgttccactt cctgcacaac atgcggatgg tgaccaatta
     2341 taagatcggc cccctcatca atcaaaaaat gagccaattc ccgttctcgc ctattggata
     2401 acggtatata ttcgtcgccc cagtgaacaa gaacgattac ggtcatctct ttttcaaccg
     2461 ttgaaattat gttttttatt ttctgccgat ccggagtgtg aaagacactg tttagggcga
     2521 ggatcgcgac tgatgtatta tttatcataa tcttttggag agaatattcg tcctcaccgg
     2581 atccgccgac accaataata ccctctgcca taaggctttt ccgggtgtct tctaaaccag
     2641 agagaccata gtcagacgtg tgattgttgg caagtgaaac tatgccaata ttatattttt
     2701 ttagaagttt tggggtggaa gacacaaatg aaaaacgcgt agaaaagaga ggagtttgaa
     2761 catgatgagc catgattgga ccctctaaat ttgcgacaac aacatcatat ttttttaaaa
     2821 actctccaac ttttgaaaaa ggatagtcat ttccgtgtcc gttttgaaga ttttcaaccg
     2881 ctcgtcccag catgatatct ccaagaaata aaatttttgt tgcgctgtct gtttcttttt
     2941 tgaacggtct ctcaatatcg acttcagaaa tccgttcata cataaataga aagagttcaa
     3001 gccctactac taccgcaaca atgtatttat ttaatcgcaa agggtacaaa tgagataaaa
     3061 aatacgagcg ccaaggtaaa attgatactc ataccggtga gcacgccgac cagtgctcca
     3121 actgcagatt tagcaatttc atgcatcttt tttttatgca tatattcgcc aaaagccaca
     3181 ccgaccacca caccgataat gccgccaaac ggcggcatta aaagaagccc aacgactgcg
     3241 ccggcaaggc caaacagcaa ggaccggcga gatgctccgc ctatatgtgc accaagcgtt
     3301 cctgaaagat aatcgacaac cagtccgaat aaaacaatta aaccgagaaa taacacattt
     3361 gagagactaa gtgtctgaaa tcgatcagca attccatata agagcgcgat aaaaaacata
     3421 tatggaattc cgggaatgat cggaataaat actcctgcta ttccgggaac cataattaag
     3481 atagtgatcg atagaagtac taactggttc atattttaga taaaaaattc agttcgaata
     3541 ttattttgtg aaaaaccaaa ctttacaaca aggtcaacca cgtgctcgac aaaagaggtt
     3601 gaaccacaaa tatacacgag cggattttta agtttgagaa tctgacgcaa gagaacagca
     3661 tcaatccttc ctttcggtcc atcccacgtg ctgtctctag tcataacggg cacaaaagta
     3721 actgcggtag ccttctttgc tagagacatt atttcgctta tatatggtag ctttttttga
     3781 ttctgggcgc ttgcaaaaag aaaaaaatcc tgacgtagag ccctgtgttc ataatgacgc
     3841 aacatggata caaacggagt gatgcctgtt ccccccgcaa tggccacgac aggttccgac
     3901 gcggatccat cccagacgaa atgcactccc aaaggaccgc ggacatccag tttgtttcct
     3961 atatttaggt ctctaagtat cggggagact tccccatttt caaaaatctc gacgcaaagt
     4021 tcaacactct ggtcgtgagg cggggacgaa atcgagtatt gtttgacggc gttatacccc
     4081 ttttgcgttt taattcggac atcaatatat tgtcccggtg tatgagtcac ccaacccgga
     4141 acacaaagac ggagtccgac taaccgctca ccaagatttg ttttctcaac tacgtttgct
     4201 tcgctccact caaaattttg agaaggaatc atgcgccaaa ttgtaccatt cgagaatcca
     4261 gtctagaaga agcttgaaaa gcgtccaata agttttcgag cacgctcttt gggaaccatg
     4321 attatgagga gaatgacgag tgcaagtaag agtagccaga aatcaaactc cccgctttgt
     4381 gctgcgaaaa atgctgcagc aaatgaattc ctattattcc cgagatcttc attcgcttcg
     4441 gccacagaaa caaggtaaga gccactcgag gtgccattta tgctgtcctc ttcagtcgtg
     4501 ccgccgagaa gtgccgcacc aagttgaata gtccgtgcta ccgaatcctg aatattgctc
     4561 gtcgtttgtg gagtagtggt ggaggcagcc gatgatgtga cgttctctcc aacttcgctt
     4621 ccgccggcac cccctcccgt aggagttgcg actgcaatcg atccggctac cgttattgtc
     4681 gaggttccct ttggtgaagt gtatgcattt ccgacgtgaa tatttctctg ccgcgccatc
     4741 gcctctacat atgcttcatt cgtatacacc cctggcaatg tcgcctcact aaatacactt
     4801 gtatacgtaa cagtaatctc ttctccggcc agaaaatctt ggagatccca ggactgttca
     4861 ttgattagtt tcccgtcctg atcgaagagt ttatcgacaa gaactgcgtc atatgcaaca
     4921 cctccctcat tatggagttt gatcatgtaa tccactgtac tcgaggcagt tatagacgtt
     4981 gtggctgaat ttgtcttcac gacggaaata atcggatcga atgcgtccca agtaacacgt
     5041 gcgtaatcat tttggcgacg cgcgagtata gaaatcgagt cagtattgtc gcttgggttg
     5101 ccatccggct catttgcggt aacagtagca gtcgagacaa catcaaaagc gcctatcggt
     5161 agggtgggtg ccacagttcc gagatagact cgctcaattg tctcacctgg gcggagatca
     5221 ccaaggtgcc attcactcgt ccatccgctt tgattttcga aagtgatgag tcttggatcg
     5281 taactatttt ttagttttac agatcgtgcc gtcgcatctc ctctgttaga tattgtgtaa
     5341 acatatcgcg cgcttccgcc cggacccaat tctccggcca cttcagctct gcttccaatc
     5401 cataggtcag gccgaccaaa cgacaaatta cccgtccaat cgccaaagat atttataatt
     5461 gcgagaatcc agttgcgtcc atatacattt gtgtttgcga gattcaacac gctcgatgcc
     5521 gcgtacgcat ttccagttga tattcccgaa tcaccgttac cgcgaatttt gttttcaccg
     5581 gtgagcgcat agaccgacac gttgtttgtg atcgcagcat tattttgagt attgattgtg
     5641 tttacactcg cactatttga attcgtttcc ccactgccca atccgtacac ttcgagtcca
     5701 tttcccgtat cgcgccactc gacattgttt ggccttccaa atacattgcc attccatcta
     5761 cctgcaatgc gaaagacaat aaagagctgg tctgtatttg tgtaattact attcagacga
     5821 ttgatgatcg aagcaaacga aagtgctcgt ccggttcgta ttcccgtgct cgtgcccgat
     5881 acttcgttgc caccagtcga tgccgaggtc gtccctgtag tagtgaccag tgcgtcgttt
     5941 gtatttgaaa cgtcgccaaa agcagaacta cttgaagccg tacttggagt gctttgcgac
     6001 gaccccgaac caccgaaact gaaattagat tcaaaataat ccgcgctcgg cagtaccaaa
     6061 tcccccgccc aatcaccaaa attgttgaat accatgagca tgtagttcga gttgacgata
     6121 tttgtattgg cgagatttat gacattggcc gccgcatagg cgtctccagt caagattccg
     6181 ccagcgccca agatattatt tccacctgtg cctgatcgaa ccaaaatatc atttgaaatg
     6241 ctcgcaatgt tattactatt tagataactc tccagactta atggcggatt actgcatcct
     6301 tctccgcagt tcgcatcttc ggtcgttgat gccataaagc gaagatcaag agatcctatt
     6361 tgcgaaaagt aattagagag aaacattagg aaaacttccg aatcgagaat attcgtatta
     6421 ataacattga cgacattcgc ttctccgtac gcggaacccg tgtcgatgga cgaatttggt
     6481 gttcccgacg ctgtgtttga tccggttgat gcttccacag tcattgtatt gcctactgtc
     6541 cctgtgttcg cgacttcgat ttcaagaggt cccgttcgcg tttcctgcga tgttgcctct
     6601 gtggtcgagg cggttgttgc gtcagtagtc gaagcagtac catcaagaga tagagctgtg
     6661 acactcggag atgttgacgt tccgtttgtt gtcgtatcgt tcgtgttgac aaaatttgtc
     6721 acttctccct gcgcttcagc atcccctgtc acaatcaccg gaccagaact ttcggtactt
     6781 gtggtttccg tgtttccctc attcgcagga ttgattgcat ccggtgcgct ttcaggctca
     6841 ggcgtttcgg atgttggcgt ttcgggtgta ggagtctccg atgttggcgg cggagcagtc
     6901 actacaggtt ctgccgcagt ttcacctccc tgctcttctg caaagccgac aaaaggcgtg
     6961 gcgaacagga acgctacgat aagacagagc gctgtcagct tttttgcaaa atcgtgtgat
     7021 tgtttaattg tatccatttt tattttggct tccgacaaga agtctcgact gatgaggccc
     7081 cttgccggaa ggaggaaaaa tcctccatcc gtagattcac tcgcttgcgc gaggcgtccg
     7141 cttccattca atctgactga atgatgattc tcatgcagtc aaagcaaata tgagacgtac
     7201 tcttatcgtc gagtaatatt tgtgttgatg ttgtttacta ctcgtgcatg tgcctcgaca
     7261 gttccggtta gaatgtctcc gcccattccg ccagttgagt catttccgcc gttaccagcc
     7321 gaaatagctc cccatgaaga tgaagacgaa acatctgcac ctcgaacttt gttctttgcg
     7381 cgtcctccat cagcaacatt caagcctgta tcagctgata cagcggcaag attgccaaca
     7441 gatgcaccgt ttcggtttcg aactcgaaca tctccctctg cagagctgct tacttctgtc
     7501 tcgttgtagt taacattgtt ctctacatga gctgaagcaa gtgcattgcc tgaagtgata
     7561 attccgcctt gtccgccagt cgctgtgttt ccaccgtttc
//
LOCUS       DATDSU010000121        16286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1950275, whole genome shotgun sequence.
ACCESSION   DATDSU010000121 DATDSU010000000
VERSION     DATDSU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16286
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950275"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1263)
                     /locus_tag="VI981_04225"
     CDS             complement(130..1263)
                     /locus_tag="VI981_04225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479531.1"
                     /translation="MKHLFYVANIRLPSERAHGIQLVKMCEAFIDSGVELTLIIPRKK
                     QSHIEEIQSYYGLRHHIHVIGLPILDFGQSRLGFNITSLSFMISSFFFLLFEKVRGKI
                     ELIYTIDLDQFSYSLLPLFAPVFIEVHGSKPPNIFLRFFFGRVRGVIAISQKVRRSIA
                     ATYKVPEEKIFEQRNGVDVALFESGLLQEESRKKAGLKLEGVIFLYSGAFYDWKGLGD
                     IIEAAETVGGEVSVVMVGGTEGELITATKRSSLPPNIICIAKQKFKNIPLWLRTADYF
                     IISGTRTNYYSFKETSPMKLYEYFTIGRPVIAARTEAIEYMTDEREVMFYEPDNAADL
                     ARVMKVAIGRDFTDMKKHAYQKASRFSLKNRAKHIMDFIASKI"
     gene            complement(1260..2300)
                     /locus_tag="VI981_04230"
     CDS             complement(1260..2300)
                     /locus_tag="VI981_04230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4479532.1"
                     /translation="MKGKKARLLVITQKVDRDDPVLGFFDEWLRSFEPLFDEINVICL
                     FRGKSVLGSKTKIWSLGKERKTSRIRYLWLFYRYVFKLRNSYDAVFVHMNVEYVLLAG
                     LWWRWTGKKIILWYNHTSGGWKTRLAFHIAHVLCHTSPYAASAGKIKSQRMPAGINTA
                     IFKEQSLPRREHSILFIGRIAPIKKVHVLVDALLILYKKNIPFFTAIYGDALPSTLYY
                     VEQLKAKAHALTKEGKLSFFSAIKNRETPSIYNSYEIFVNLTPRGNFDKTVLEALACG
                     TTVLASSDAFYEVLEEPYRFIEDDPEELARKIEHVFRTPRNKETRERHSAYVKKEHEL
                     SYLGLRLRKLIQ"
     gene            complement(2569..3444)
                     /locus_tag="VI981_04235"
     CDS             complement(2569..3444)
                     /locus_tag="VI981_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479533.1"
                     /translation="MRNQNKKQMKQKEKIGFIGQGWIGKNYADNFEKRGFEVVRYSME
                     EPYVRSKEEIKKCDIVFIAVPTPTTKRGFDDKIVDSVLTHIGRGKTAVIKSTTLPGTI
                     KKLQKKYPDITILHSPEFLTKATAAYDTSNPARNIVGIPTNTPSNKKKAKYVLSILPR
                     APVELICSSDESELIKYGSNCFYYTKVVFFNILFDLAKKLDLEWKPVELALKADPMIG
                     KSHVEPLHKSGRGAGGVCLLKDFEAFSQLVNRELGAKEINLMRAIRDKNLALLALTRK
                     DEEFVRMIYGRGSRK"
     gene            complement(3455..4627)
                     /locus_tag="VI981_04240"
     CDS             complement(3455..4627)
                     /locus_tag="VI981_04240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479534.1"
                     /translation="MQKKRALVMISTDRSIFQRYSQARARMVEYGELVDELHIIIFAR
                     KALGFAEMTLSSQVKIYPTASWSKAGYMSDALSSAKRILEGLPRELYDVALSAQDPFE
                     TGLVGRQLKKKFKLPLQIQVHVDFLSPFFRRGPVLNYVRIPLAKEVIKYADQVRVVSL
                     RIARSLESLVEKKKIKILPIFVDIESGANHNGAEQSKIIFNRFSHVILMISRLAPEKN
                     FACALEAFSRLLRTYPKALLVIVGSGPEKANIEYIAHDLGIARSVILTGWQGAVRPYY
                     KKAKLFLSTSWYEGYGLALVEAAAAGIPIVTTNVGIVGDVLVDGESALVCDVNDADCL
                     TIKMTTLIKDQELEQRLKTNAARALGKHLARNRKDFLMAHAELFKDLFNVTELASS"
     gene            complement(4637..5755)
                     /locus_tag="VI981_04245"
     CDS             complement(4637..5755)
                     /locus_tag="VI981_04245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4479535.1"
                     /translation="MRHNILIATGIYPPSVGGPATYSFFLFHELPKMGLQTLVYSFDE
                     VRKLPKVIRHIQYFLGVLRRGFGVRMIYAQDPVSVGLPALFAARLLRKPFIIRIAGDY
                     AWEQATQRFDVHEHLDTFAGKDGPYPFFVRILKRVQAYVATRADVVIVPSQYLKQVLV
                     KWGVKPQNIRVIYNSVDVPFFDTKANLRKKFNLEGCVIFSAGRLVQWKGFNALIEIMP
                     EILKKDKGCSLFIAGDGPERPTLKRLIAKYKLEDCVFLVGKLPREEVLEFVKASDIFV
                     LNTAYEGFSHVALEVMAVGTPLITTRAGGNTELIEHMHDGLLVEYNDHKALRDAIVTI
                     KDDPIFAQTLVQNARKKLERFSKEKMIKELVAQLGESL"
     gene            complement(5761..6906)
                     /locus_tag="VI981_04250"
     CDS             complement(5761..6906)
                     /locus_tag="VI981_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4479536.1"
                     /translation="MYYPRFVGGAEIAIGEVTKRIDPKLYEFHLITLGLDSSFPKQEK
                     IGNVHVYRIGQTREDMTAAELRRPLWYLVKMLYPVVALFRAIELHRKIKFDFTWAIMT
                     YMSLPALLFKWLKGVSYVITLQDGDSPEHITGRKRIFLLRPFLRAAFRGASDIQCISN
                     YLSGFAKKMGSKIEPKVIPNGVDTRVFKRDPTTVLKQELHLGGSKIIITTSRLVQKNG
                     IDILIESLVYLPKDIKLLIVGQGELREKLEALVLERALSGRVVFLGHVPYEMIPLYLS
                     LADVFARPSRSEGMGSSFIEAMAVGIPVIGTAVGGIVDFLRHRETGLIVPVNNPRALA
                     ESISLVLNDEKLCKKLVEHAQDMIQRNYAWDRVADSIKKMFDGVRFL"
     gene            complement(6966..8408)
                     /locus_tag="VI981_04255"
     CDS             complement(6966..8408)
                     /locus_tag="VI981_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY4479537.1"
                     /translation="MKYAFLFSVLLFPLTFAHASLVITEIMYDLEGSDTDREWIEIKN
                     NSSANIDPALYKLYEANTNHGLKVYQGALEIPAGGYAVITDSPAKFLADWPNFSGIVI
                     DSSFSLSNDGETLEIKLEEVTEHTVSYMKTDGAAGDGNSLHFDSGTRALAPTPGTGVE
                     NAGGTPPSTPSSPSPSSAGGGSTSNPVSNSSSQSSPSSKSTIFAEIQTERSTLVGGIV
                     TFKGTAYGLERQPIANARYLWNMGDGTTKEGMQIEHIYTHPGSYTVVLDVSSGEYGAS
                     QKVSIDVSEAKISVSDFVEGPSGFLSLKNDSNFEVSLSGWHLRGSKVFTLPNKTYVGA
                     HKTLMLSNDVTGLGTERMIELLYPNGALALTYTKKNLVAPAPMPAPPPVASAKPAVRK
                     TEPKSDTTLASGEKKKEATATPIARTVENNGQTAAVLLASEESSRTPASSMIWIASLV
                     GVICLGVPLALHFSRRKEEEGPASDITIID"
     gene            complement(8457..10229)
                     /locus_tag="VI981_04260"
     CDS             complement(8457..10229)
                     /locus_tag="VI981_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEY4479538.1"
                     /translation="MDENQITYFAYTDSRNKKIPFGIRRKDRSRHVYIIGKTGMGKST
                     LLENMAVQDVKNGEGLAFIDPHGKTAELLLDYIPEDRIKDVLYFAPFDLDYPVAFNVM
                     EDVGHEKRHLVANGLMSAFKKIWVDAWSARMEYILNNILLALLEYPDATLIGVNRMLA
                     DKEYRKKVVDNVTDPSVKAFWIDEFARYGDRYMQEAGAAIQNKIGQFISNPLVRNIIG
                     QPKSTFDLRKFMDAKKILIVNLSKGRVGEANANLLGSMIITKIYLSAMSRADVEDSKL
                     RLLPNFYLYVDEFQSFANESFADILSEARKYKLNLIMAHQYIEQMSEEVQAAVFGNVG
                     TMIVFRVGAFDAEVLEKEFAPQFSIEDLVNLGFVQVYMKLMIDGVTSPPFSAVTMPPI
                     PVSETSYKHQIIESSRSLFAQPRAKVEEEIKRWHQPIRDVRVLEKDRETREYRESARS
                     GTVRNELERMVPQGMNRFSRGDERHREERPRAEDQRVSKPEPTRHVDREAELRKVMSL
                     DYLKAKQPPKNDHKERSKENVSALRVALSGILKNTNTDQSKRSDVLASMAPETKKDEP
                     PAQNFPHTPKEVPEELLRNVLRDT"
     gene            complement(10493..10687)
                     /locus_tag="VI981_04265"
     CDS             complement(10493..10687)
                     /locus_tag="VI981_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479539.1"
                     /translation="MKDSTIPVGSGTAGTARRADSTPNRNKAADIRTPLLLNELGFLT
                     AAVPKQTGAATVAEWIEVVR"
     gene            complement(10932..12065)
                     /locus_tag="VI981_04270"
     CDS             complement(10932..12065)
                     /locus_tag="VI981_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEY4479540.1"
                     /translation="MIVVLVALGFFVFSRYSELSYSRGGIVLQASDSVKEPVEAVKIV
                     KEIKHVSTPEPLRAIYMTSWVAGTDKWMKELVDLVDSTELNALVVDIKDYTGAISYEI
                     TDENLLKFATFEPRIPDIESFISYLHDRNIYVIGRVSVFQDPMLVKKRPDLAVKEKSN
                     PERIWKDYKGISWLDAGAVEVWDYAVMIAQDAYKRGFDEINFDYIRFPSDGDMKNIYF
                     PWSGGDEKHLVLREFFKYLHEKLSDQPLVMSADLFGLTTTDTRDLNIGQILEDTFPYF
                     DFVAPMVYPSHYPNNFNGFPNPAEKPYELIHYVLESAVRRAEIASTSPHKIRPWLQDF
                     NLGATYDAVKVRAQIQATYDVGLTSWMLWSAANRYTKGALLPK"
     gene            complement(12109..12357)
                     /locus_tag="VI981_04275"
     CDS             complement(12109..12357)
                     /locus_tag="VI981_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479541.1"
                     /translation="MTLKAKIKTLLFIGVILILLQIAGIPADFKKILYGIFGIIIVWI
                     AASLYRVADESRTEKRTTSFVESRMSDITPPKDPAHGS"
     gene            complement(12415..13101)
                     /gene="rsmI"
                     /locus_tag="VI981_04280"
     CDS             complement(12415..13101)
                     /gene="rsmI"
                     /locus_tag="VI981_04280"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY4479542.1"
                     /translation="MANKTFYVVATPIGNLEDITIRALRILKEVDLILCEDTRVTKKL
                     LTAYEIKTPTLSYHTHSKLTKLEHIMRLMEEGKTLALVSDAGTPAISDPGTVLVRELA
                     RRFASEIKIIPIPGPSAYTALLSASGLPCSEFVFIGFLPHKKGRETIFKSMHDETKTV
                     VFYESPHRIMKTLFSLLALGEKRSVVVGRELSKMFEEIVRGTPGEVHNFFTDHPDHIR
                     GEFTVAVGPV"
     gene            complement(13175..14086)
                     /locus_tag="VI981_04285"
     CDS             complement(13175..14086)
                     /locus_tag="VI981_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479543.1"
                     /translation="MKTSPTRVFNPFPNVKVRLFGRNSGNKTDWSITPLTDEARQVGG
                     TMPDCYDLVAAQLYEADVHTAYAPRPSMFNAEIVDHGELKTFHQPNFDTHVEDELFPW
                     RRSVGIFRGVDADGCDIPSGCAFWLSSADCSCIVARAKSGFTIAAHAGRDSLVDRKCL
                     ETDTPSRRHASVVDAIMARFKENGDLTADLKFFLTCGIRADAFRHRSDHPKFGQSNRK
                     LIEHLRLLDPMCVLGDPCEGRISLNAVVIAQLGRHGVSMSQITDDNIDTFADKGLGGE
                     YTWWSNARATTLEEKAKRNGVMVINRR"
     gene            complement(14202..15527)
                     /locus_tag="VI981_04290"
     CDS             complement(14202..15527)
                     /locus_tag="VI981_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY4479544.1"
                     /translation="MIDFKKVNSVYFIGIGGIGVSAIARMFLLENKEVHGSDLSESEI
                     TEELQKLGAKITIGQGVELIPEGIDLIIYTVAIEKYAVDFLAQIKALAISSLSYPQAL
                     HEISNGKYTIAVAGTHGKTTTTAMIAKILIDAGLDPTVIVGSLLQGSTLDREKGRTFG
                     TNFIAGKSKYLVVEADEYRRGFLNLTPSILVITVIDEDHLDYFKDLSDIQSAFKELIA
                     RMPLKGIIIANKENRNVVAVLEGLPQKILFFETPALEEFTLLFPGEHNRRNAAAALHV
                     ATELGIEREKVKIFLAAFQGTARRFEYKGKMKSGALVYDDYAHNPQKISALIQGVREK
                     YPNKTLTVVFQPHLYSRTKDHLDAFAESLSVADHVILAPIFPAREKFDPSISSRMLAE
                     KIRPLNENVRCLDTFEMITDDLEDSTSRDGVVVTVGAGDIYKVAELLLK"
     gene            complement(15727..15906)
                     /locus_tag="VI981_04295"
     CDS             complement(15727..15906)
                     /locus_tag="VI981_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479545.1"
                     /translation="MRLFQIVSHVCPDCGEVLGSHRYIPAWFVVLCTILIPGFIFLCL
                     WFAGYLLSALDRFLR"
     gene            complement(16009..>16286)
                     /locus_tag="VI981_04300"
     CDS             complement(16009..>16286)
                     /locus_tag="VI981_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479546.1"
                     /translation="NEPPPVGVTAYAKNGVLATTNGLPFTVEGNTVIMRDPQGYGETL
                     RSVVEFSRDLITWTPLVTIDLPEGQKVRIQDDSGPERAFYRFRLVAP"
BASE COUNT         4313 a         3862 c         3537 g         4574 t
ORIGIN      
        1 cccctttcac aaaaacgata tactcgttgt ctcgatcaac gctaagcagt gctcttgccc
       61 agccttcaat aatccgcggt agcccgccag gcattttttc tccgtatggg tgaagtacaa
      121 tgccaatctt catatttttg aagcgataaa atccattata tgtttggcac gattttttag
      181 actgaaacga gaagcttttt ggtacgcgtg ttttttcata tccgtaaaat ctcttcctat
      241 cgcaaccttc ataacgcgtg ctaagtcagc cgcattatct ggctcataaa acatgacttc
      301 gcgttcgtcg gtcatatatt cgattgcttc ggttcgtgca gcaatgaccg gccgccctat
      361 tgtaaagtat tcgtataatt tcataggtga tgtttcctta aatgagtagt agtttgtgcg
      421 cgtacctgaa ataatgaagt aatccgcggt tcgaagccaa agcggaatat ttttaaactt
      481 ctgcttggca atacagataa tatttggggg taacgacgag cgtttagtgg cagtaatgag
      541 ttctccttcc gtgccaccga ccatcactac ggatacctct cccccgactg tttctgcagc
      601 ctcgatgata tcgcctaaac ctttccaatc gtaaaacgca ccgctataca aaaaaattac
      661 gccctccaat ttaagtcctg ccttttttcg actttcttct tgaagcaacc cactttcaaa
      721 cagcgccaca tcgacgccgt ttcgctgttc aaaaattttt tcctccggca ccttatatgt
      781 tgccgctatt gatcgcctca ctttttggct gatggcaatt acgccgcgca cacgcccaaa
      841 gaaaaaacgc aagaaaatgt tcggcggctt tgagccgtga acctcaataa acactggtgc
      901 aaaaagcggc agtaaactgt acgaaaactg gtcaaggtcg atggtgtaga ttagttctat
      961 ttttccacgg actttctcaa atagcagaaa gaaaaaagat gagatcatga acgagagcga
     1021 cgttatgttg aatcccaaac ggctctgccc aaagtcgagt atcggtaatc ctatgacatg
     1081 aatatggtgc cgaagaccat aataagactg tatttcttct atatgacttt gcttcttcct
     1141 tggaataata agtgtgagct caacaccaga atcaataaaa gcctcacaca tcttaacgag
     1201 ttgtattccg tgggcccgct ccgaaggtag ccttatattg gcgacataaa aaagatgttt
     1261 cattgaataa gttttctaag tcgtaatccg agatatgaaa gttcatgctc ttttttcacg
     1321 tatgcggaat gccgttcacg agtttcttta tttctcggcg tacgaaacac gtgctcgatt
     1381 tttctcgcaa gttcctctgg gtcgtcctca ataaatcggt atggttcttc aagcacttca
     1441 taaaaggcat cgcttgaggc aagtaccgtc gtaccgcagg cgagtgcctc aaggacggtt
     1501 ttgtcaaaat tgccgcgagg agtaagattg acgaaaattt catacgaatt gtaaatagaa
     1561 ggagtctcgc ggtttttaat agcagagaaa aaagacagct tgccttcttt tgtcagtgca
     1621 tgagcttttg ccttgagttg ttccacataa tatagagtac ttggaagcgc gtcgccgtag
     1681 atggctgtaa agaatggaat atttttttta tacaaaatca gaagggcgtc gacgagcaca
     1741 tgaacttttt ttatgggagc aattcttccg ataaaaagaa tcgaatgttc gcgccgcggc
     1801 aaactctgtt ctttgaatat ggccgtattg attccggcgg gcatacgctg cgattttatt
     1861 tttcctgcac tcgccgcata tggagaagtg tgacaaagca catgagcaat atgaaatgcg
     1921 agacgtgtct tccaacctcc gctcgtgtgg ttataccata ggattatctt tttacccgtc
     1981 caccgccacc ataaccctgc aagtagaaca tactcgacgt tcatatgtac aaaaactgca
     2041 tcatacgaat ttcgcaattt aaaaacgtat cgataaaaaa gccacagata gcgtattctc
     2101 gaggtttttc tctcttttcc gagcgaccaa attttcgttt tactcccgag aacactctta
     2161 cctctaaata gacagatgac attgatttca tcgaacagag gttcaaacga tcgaagccat
     2221 tcgtcaaaaa aaccgagcac gggatcatca cggtcgacct tttgcgttat gacaagcagt
     2281 ctagcttttt ttcctttcat cccgttaaaa aattaatatt tttaaactgt ggtacactcg
     2341 ttttttgtat cttgtatgtt ggatattttg gaaatcacgt aaagttgaat tttttaacgg
     2401 gacaggtccc tcagtctcga attcctaaat cccgttagaa gttcttactt ctaatcggga
     2461 ctaacgggat ccacggggta gtgaaaaatg acatcgcgta gaacaaagat acaaatacgt
     2521 tgccaaatta agtttcatat atttttcata gctcatcgaa aacttacatc attttctact
     2581 accccgtcca taaatcatgc gaacaaactc ttcgtctttt cgagtcagcg ctaaaagcgc
     2641 cagatttttg tctctaattg ctcgcattaa gtttatctct tttgcgccaa gttcacgatt
     2701 gaccagttgt gaaaacgcct cgaaatcttt gagaaggcag acaccgcccg cgccgcggcc
     2761 gctcttatga agcggttcga catgactctt tccaatcata ggatcagcct tcaatgctaa
     2821 ttcaactggc ttccattcga gatcgagttt ttttgcaaga tcgaaaagta tattaaagaa
     2881 aacaactttt gtgtaataaa aacagttgct gccatattta atgagctctg actcgtctga
     2941 cgagcaaata agctcgaccg gagctctcgg caaaattgaa aggacatatt tggccttctt
     3001 tttattgctc ggagtatttg tgggaattcc gacaatgttt cgcgccggat tacttgtgtc
     3061 atatgcagct gtggctttgg taagaaattc aggtgagtga agaattgtaa tatctggata
     3121 tttcttttgc aattttttta tcgtcccagg aagggttgtt gattttatga ctgccgtctt
     3181 tccccgacca atatgagtca aaaccgaatc gacaattttg tcgtcgaaac cccgtttcgt
     3241 ggtcggtgtt ggaacggcaa tgaatacgat atcgcatttt tttatctctt ctttactcct
     3301 aacgtacggt tcttccatac tgtatcgcac gacctcgaaa ccgcgcttct cgaaattgtc
     3361 agcatagttc ttgccaatcc acccctgccc gatgaatcct attttttctt tttgcttcat
     3421 ttgtttcttg ttttgatttc tcatttgtac ataactacga ggaggcaagc tccgttacat
     3481 taaaaagatc tttgaaaagc tccgcgtgag ccattaaaaa atcttttctg ttcctggcga
     3541 gatgcttgcc gagggcgcgg gccgcatttg ttttgagtct ttgctcaagc tcttggtcct
     3601 ttataagtgt agtcatcttt atggtcaagc agtcggcgtc attaacatca catacaagag
     3661 cactctctcc atcgactaaa acatctccga caattccaac gtttgtggtc actatgggta
     3721 ttccagcggc agctgcttcg acgagcgcca aaccatagcc ttcataccat gatgtggaca
     3781 gaaaaagctt tgctttctta tagtacggac gcacagcacc ctgccagccg gtcagtatga
     3841 ccgaacgggc aataccgaga tcatgtgcaa tatattctat attagctttt tcaggaccgc
     3901 ttccgacaat aacgagaagt gccttggggt acgtccgcaa aagccttgaa aatgcctcga
     3961 gggcgcaggc aaaatttttc tctggtgcaa gccgggaaat cataaggata acgtgagaaa
     4021 acctgttaaa aatgattttt gattgttccg ccccattgtg atttgcgcca gattctatat
     4081 cgacaaatat ggggaggatt tttatttttt tcttttcgac aagtgactca agtgaccgtg
     4141 caatacgcag gctgaccact ctcacctgat cggcatattt tataacttct tttgcaagcg
     4201 gtatccgcac atagtttaaa actggaccgc gtctgaaaaa tgggcttaaa aaatctacat
     4261 ggacttgaat ttgaagcggc agtttgaatt ttttcttcaa ctgccttccg acaagccccg
     4321 tctcaaacgg gtcttgtgcg gagagtgcaa catcgtaaag ctcgcgcgga agtccttcga
     4381 gtatccgctt cgccgaggag agcgcgtcgc tcatatatcc cgctttactc cacgaggcag
     4441 ttgggtagat tttcacttga gaacttagcg tcatttccgc gaacccaagt gcttttctcg
     4501 cgaaaataat aatatggagt tcatctacaa gttctccgta ttcaaccata cgggcccgag
     4561 cctggctgta tctttgaaaa atcgaacgat ccgtcgagat cataacgagt gcccgttttt
     4621 tttgcatgtg cacagcttac agactttcgc caagttgcgc aacaagttct tttatcattt
     4681 tttctttgga aaaacgttcg agtttctttc gcgcattttg tactaacgtt tgagcaaata
     4741 ttggatcatc tttgattgtg acaatggcat cacgaagcgc tttatgatca ttgtattcaa
     4801 caagcaaacc gtcgtgcata tgttcaatta gttccgtatt gccgcctgcc ctcgtggtga
     4861 taagtggcgt tccgaccgcc ataacttcga gcgccacatg tgaaaaaccc tcgtaggcgg
     4921 tattcaatac aaaaatatcg gaagccttta caaactcaag cacttcttca cgcggcaatt
     4981 tacccactag aaaaacgcaa tcctccagct tgtatttcgc gatcagtcgc ttaagcgtgg
     5041 gcctctccgg cccgtcacca gctataaaaa gagaacagcc tttatctttt ttcaatattt
     5101 ccggcatgat ttcgataagt gcgttgaatc ccttccactg taccagtcga ccggcggaaa
     5161 agatgacgca tccctccagg ttgaatttct ttctcaagtt cgctttcgta tcgaaaaaag
     5221 gaacatcaac gctattgtaa atgacgcgaa tattctgggg tttgactccc cactttacaa
     5281 gcacttgctt taaatattga gaagggacaa taacaacgtc ggctcttgtg gcgacgtatg
     5341 cttgaacccg ttttaaaatt ctgacaaaaa aaggatatgg gccgtctttg cccgcaaacg
     5401 tatcgagatg ctcgtgaaca tcaaaacgtt gagtggcctg ctcccatgcg tagtcgcctg
     5461 cgattcgaat aataaagggt tttcgcaaga gacgtgcggc aaaaagagcc ggcagtccga
     5521 cacttaccgg atcttgagcg tagatcatgc gcacaccaaa cccccgtcgc aacacaccca
     5581 aaaaatactg gatgtggcgt atgaccttgg gcaatttcct tacctcgtcg aaactgtaga
     5641 cgagtgtttg aagacccatt ttcggcaatt cgtgaaagag aaaaaaagaa tacgtcgccg
     5701 gcccaccaac tgaaggcgga taaattcccg tagcaatgag tatgttgtga cgcatagtta
     5761 ctagagaaac ctgacaccgt caaacatttt ttttatcgaa tcggccacgc gatcccacgc
     5821 atagttcctt tgtatcatat cttgtgcatg ttcgacaagt tttttgcaaa gtttctcatc
     5881 attaagtact aaactaatgc tttccgcaag tgcgcgagga ttgttcactg gcacaatgag
     5941 tcccgtctct ctgtggcgca aaaaatctac aattcctccg acagcagtgc cgatcaccgg
     6001 aataccgaca gccattgctt caataaacga acttcccatg ccttcagatc gtgatggacg
     6061 tgcaaagaca tctgctagcg acaagtagag tggtatcatt tcgtacggta catggccgag
     6121 aaatacgacc cgaccgctga gagcgcgctc gagcacgagc gcttctagtt tctcccgaag
     6181 ttcaccctga ccgacaatga gaagttttat atctttcggc agataaacca gtgattcgat
     6241 cagtatgtct attccatttt tttgaacgag gcgcgacgtt gtgataatga ttttcgatcc
     6301 acccaagtga agctcttgtt tgagcaccgt cgtcggatcc cttttaaaga ctctcgtatc
     6361 aactccgttc ggaatgactt tgggttctat ctttgaaccc attttttttg caaatccgct
     6421 taggtagttt gaaatacact gtatgtcgct tgcacctcgg aacgcagctc tcaaaaacgg
     6481 gcgcagtaaa aaaatacgtt tgcggcccgt aatatgttcc gggctatctc cgtcctggag
     6541 cgttatgaca tacgacacgc ctttcaacca tttgaagagc agtgccggca gcgacatgta
     6601 ggtcataatt gcccacgtga aatcaaattt aatctttcga tgcaattcga tcgctcgaaa
     6661 cagagccaca acgggataga gcatctttac gagataccat aaaggtcttc ggagctccgc
     6721 tgcagtcata tcttctcttg tctgaccgat tctatataca tgaacattgc cgattttctc
     6781 ctgcttcgga aacgaggaat cgagaccaag tgtaatcaaa tggaattcat acaatttcgg
     6841 atcaattcgt ttggttacct cgccaatggc aatttccgcg ccgccgacaa agcgcggata
     6901 gtacatgagt gaaaatatta ggattttttt cctatccatc tgttcaattt aaatttactt
     6961 aaaacttaat cgatgatcgt aatgtcagat gccggccctt cttcctcttt tcttctcgaa
     7021 aaatggagcg caagtggtac tccaagacaa ataaccccca caagtgaagc aatccagatc
     7081 atagaactcg cgggtgttcg actagattcc tcactcgcaa ggagcacggc tgcagtttga
     7141 ccattattct ctacagttcg agctatcgga gttgctgttg cctccttttt tttctcacca
     7201 ctcgccagag ttgtgtccga ctttggttca gtttttcgaa ctgcgggctt tgcactcgca
     7261 accggaggag gcgctggcat aggtgccggg gctaccagat ttttttttgt atatgttagg
     7321 gcgagagcgc cattcggata taggagttcg atcatacgtt ctgtgccaag tccggtcaca
     7381 tcattgctaa gcattaatgt cttgtgcgca ccgacataag tcttgttggg aagcgtgaat
     7441 acttttgaac cccgcagatg ccatcctgaa agcgacactt cgaaattact gtcatttttg
     7501 agtgagagga aaccgcttgg accttccaca aagtcactaa cgcttatttt tgcttccgag
     7561 acgtctatgg acactttttg gctcgcacca tactctccgg aggatacatc aaggactact
     7621 gtataagaac ccggatgagt gtaaatatgt tctatctgca ttccttcttt tgttgttcca
     7681 tcacccatat tccaaagata tcgagcgtta gctatgggtt gccgctcgag tccgtatgcg
     7741 gttcctttaa aagtcacgat gcctccgacg agcgtgcttc gctccgtttg tatttctgcg
     7801 aaaatagtgg atttagaact tggagatgat tgggaagagc tgtttgatac cggattactt
     7861 gtacttcctc cgcccgcaga cgacggagag ggtgacgacg gggtcgaagg aggggtaccg
     7921 ccagcatttt ccaccccggt tcctggggta ggcgcaagtg ctctcgtccc cgaatcaaaa
     7981 tggagactgt tgccgtcccc agctgcacca tcagttttca tataagaaac agtgtgctcg
     8041 gtcacctcct caagtttaat ctcgagagtt tcgccgtcat tacttaggga aaaactacta
     8101 tcgattacaa tgcccgaaaa attcggccag tcagctaaaa attttgcggg tgaatcagtt
     8161 ataacggcgt atccgccggc aggaatttca agcgctcctt ggtatacctt taacccgtga
     8221 tttgtgtttg cctcgtagag tttatagagc gccggatcaa tgttggcgct actgttattt
     8281 tttatctcaa tccactcccg atcagtatca ctgccctcga gatcatacat aatttctgtg
     8341 atgaccaaac tcgcatgcgc gaacgtaagc gggaaaagca agacagaaaa caaaaaagcg
     8401 tatttcatgg ttgtttttgg aatggcagaa aaatttgaaa tctcaaaata acttcgttac
     8461 gtgtctctaa gcacgtttcg gagcaattcc tctggaacct ctttcggggt atgagggaaa
     8521 ttttgcgctg ggggttcgtc ttttttcgtc tccggcgcca tggaggcaag cacgtcagag
     8581 cgtttcgatt gatctgtatt tgtgtttttc aaaattcctg acaatgctac acggagtgcc
     8641 gaaacatttt cttttgagcg ttctttatgg tcatttttag gcggctgttt ggcttttaaa
     8701 taatcgagtg acataacctt gcgcaattcc gcttcacgat ccacatggcg agtgggctcc
     8761 ggttttgaca ctctttgatc ttcagccctc ggtcgttctt ccctgtgtcg ctcatctccc
     8821 cttgagaaac gattcattcc ttgtggaacc atgcgttcaa gttcattccg aacagtaccg
     8881 ctacgtgccg attctcgata ttcgcgcgtc tctctgtctt tttcaagcac tctcacgtcg
     8941 cgaatcggtt ggtgccatcg cttaatttct tcttcaactt ttgccctcgg ttgagcaaat
     9001 aatgagcggg aactttctat aatttggtgt ttatatgaag tttctgacac tggaatcggc
     9061 ggcatggtga cagccgaaaa aggcggtgag gtcacaccgt caatcataag tttcatatag
     9121 acctgaacaa aacctaggtt gacgaggtct tcaatcgaaa actgcggtgc aaattctttt
     9181 tcgagtacct cagcgtcaaa agccccgaca cggaacacga tcattgtgcc gacgttgcca
     9241 aatacagccg cctgcacttc ctctgacatc tgctcaatat actgatgagc catgatgaga
     9301 ttcagtttgt atttccgggc ttcggagagg atatcggcaa atgattcatt agcaaaagac
     9361 tggaactcgt caacatagag ataaaagtta ggcaaaagtc tcaatttact atcttcaacg
     9421 tcggcacggg acatggcaga gagataaatt tttgtaatga tcatactgcc aagaagattg
     9481 gcgttagctt cgccaacccg cccttttgag aggttgacga tcaatatttt ttttgcatcc
     9541 atgaacttcc gaagatcaaa tgtggatttc ggctgaccga tgatatttct aacaagcgga
     9601 ttcgaaatga attggccaat tttattttga atggccgctc cggcttcctg catatatcga
     9661 tcaccgtagc gcgcaaattc atcgatccag aaagctttga ccgaggggtc tgtgacgtta
     9721 tcgacgactt tttttctata ttctttgtcc gcaagcattc ggttgacacc aatcagcgtt
     9781 gcgtccggat attcgagaag cgcgaggaga atattattta aaatgtattc cattctggcc
     9841 gaccatgcat ccacccagat cttcttaaag gcgctcataa gaccgttcgc cacaagatga
     9901 cgtttctcat gtccgacatc ttccatgaca ttaaaagcga cgggatagtc gagatcgaaa
     9961 ggagcaaagt acagaacatc ttttatgcgg tcctctggaa tgtagtcgag gagaagttcg
    10021 gccgttttcc catgcgggtc gataaaagca agaccttcgc catttttaac gtcctgcaca
    10081 gccatgtttt caagaagggt agattttccc attccagtct taccgataat gtacacgtgg
    10141 cgggaacgat ccttacgtct gataccgaat gggatttttt tatttctcga gtcagtatat
    10201 gcaaaatatg taatttgatt ttcatccatg gggtagtgaa aaatggcatc gctctattac
    10261 agagcattac tgtgccttaa attcagcata tgaaaaataa tttgtatgtg tgtattgtat
    10321 gtaaatcatg tcctctaatg ccattttcta ctaccccatc cacagctttc atgatacccg
    10381 ttttcgaggc atttttttaa gtataccttt tttgaagtat agataaaaat caaaaaatat
    10441 cggaaaaaag cgcccgaagc tattgctgcg ggcacgtgaa cacctagagg cgttagcgaa
    10501 ccacttcgat ccattccgca accgttgcgg cacctgtctg tttcggcacc gcggccgtca
    10561 gaaacccgag ctcgttcagt aacaacggcg ttctgatgtc ggcggccttg tttcggttgg
    10621 gagttgaatc tgcacgtctg gcagtaccag cggtacccga accaacggga atagtgctat
    10681 ccttcatggt tttttttatt tgtggttatg tttgtgagcg cgcatccttt cgcgaccccg
    10741 caaaaagacg ttctcggcta tttcaatgtc cgatattata tcaaaataaa ataatagtca
    10801 agtcaaggcc tgtgtagatt tataccccca ttccccgccc ccgaacagct cgattcgaag
    10861 aatagggccg acgcctttgg cgaaaagaag gttgcacccg cttcagagtc acattaatag
    10921 agaatcgtgc actactttgg cagcagtgcc cccttggtat accgattggc tgctgaccag
    10981 agcatccacg aggtcagacc aacatcataa gttgcctgaa tttgggccct cactttcacg
    11041 gcgtcatagg ttgcgccgag attaaagtcc tgcagccacg gcctgatttt atgcggtgac
    11101 gttgaagcga tctcggctct ccgaacggcg ctctcgagaa catagtgtat gagttcgtat
    11161 ggtttttcag ccggattcgg aaaaccatta aaattattgg gataatgaga cgggtaaacc
    11221 atgggcgcga cgaaatcaaa atacggaaaa gtatcttcaa gtatctgacc gatattgagg
    11281 tcacgggtat ccgttgtggt aagaccaaat aaatccgccg acataacaag cggctggtca
    11341 gagagtttct catgcagata tttaaaaaat tctcggagca cgaggtgttt ttcgtctcca
    11401 ccgctccaag gaaagtagat atttttcatg tctccatcag atggaaaacg aatgtaatca
    11461 aaattaattt cgtcaaaacc cctcttgtag gcgtcttgcg caatcatgac agcgtagtcc
    11521 catacttcta cggcaccagc atcgagccaa gagatccctt tatagtcttt ccagattcgc
    11581 tcgggattac tcttctcctt cactgcaaga tccggccttt ttttgacgag catcgggtcc
    11641 tgaaagacag agactctgcc gatgacatat atatttcgat cgtgtaagta tgaaataaaa
    11701 gattctatat cgggaatgcg cggctcgaaa gtggcaaact tcaaaagatt ttcatcggta
    11761 atttcgtatg aaatggcgcc tgtgtaatcc ttgatgtcga caacaagcgc attgagttca
    11821 gtcgaatcta cgaggtcaac aagttccttc atccacttat cggtgccggc aacccaactc
    11881 gtcatataga tagccctaag tggttctgga gttgagacgt gttttatttc ttttactatc
    11941 tttactgctt ctaccggttc tttgacacta tcagacgctt gaagcacaat tccccctctc
    12001 gaatatgaga gctcagaata tctcgaaaat acaaaaaaac cgagtgcaac caaaacaact
    12061 atcacgagac atagtataga aaagtaaaaa atagatgcgc gactttcttt atgagccatg
    12121 cgccgggtct ttaggcggag tgatatcgct cattctgctt tcaacaaatg aagttgttct
    12181 tttttccgtt cttgattcgt cagccaccct ataaagtgac gcggcaatcc aaacgataat
    12241 gattccaaat atgccatata aaattttctt gaagtcagcg ggaatcccgg cgatctgcaa
    12301 caagataagt atgacgccga taaataatag cgtctttatt ttagccttta acgtcatgac
    12361 cctaataata gcttagtcct ctcttttcac aatcaggttt cgctgtggat aggtttatac
    12421 gggtccgacc gctacggtga attctcctct tatatgatct gggtggtccg tgaaaaaatt
    12481 gtgtacttca ccgggagttc cacggacaat ttcctcaaac atcttagaaa gctcgcgccc
    12541 cactacgaca gagcgcttct ctccaagtgc cagcaaggag aaaagggtct tcatgattcg
    12601 gtgtggcgat tcataaaaaa caactgtttt tgtctcgtca tgcatgcttt taaatatggt
    12661 ctcgcgtccc tttttgtgag gcaaaaaacc aataaaaaca aattccgaac agggcagtcc
    12721 ggaggcggaa agcaaagcag tataggccga cggtccgggg atgggaataa tttttatttc
    12781 tgaagcaaaa cgtcgagcga gctcgcgaac gagaaccgta cctggatcag aaattgccgg
    12841 cgtgcctgca tccgaaacga gcgcaagcgt ttttccttct tccatgagcc tcattatatg
    12901 ttcgagtttt gtaagtttgc tatgcgtgtg gtaactcaat gttggagttt ttatttcata
    12961 cgcagttagc aatttttttg tcacccgcgt atcttcacac aaaatcaaat ccacttcctt
    13021 aagtatccga agcgctcgga ttgtaatatc ttccaaattc cctatcggtg ttgccacaac
    13081 atagaatgtt ttgttggcca tacctgccga ctataacaaa ctcgaaaaga aaaaaacagc
    13141 gtgagacccc tcgcgctgtt gtgaaaaacc gaggtcacct acggttgatg accatcacgc
    13201 cattcctctt cgccttttct tcgagcgttg tagcccgagc gttggaccac catgtgtact
    13261 ctccaccgag acctttgtct gcgaaggtat caatattgtc atcggtgatc tggctcattg
    13321 aaacgccgtg cctgccaagc tgagcgataa cgaccgcgtt caagctaata cgcccttcgc
    13381 atggatcacc gagaacgcac attggatcga gcaaacggag gtgctcgatg agcttccggt
    13441 tcgattgccc gaacttcgga tggtccgagc ggtgacgaaa tgcatccgcg cgtattccac
    13501 aggtgaggaa gaacttcagg tcggctgtca ggtcgccgtt ttccttgaat cgcgccatga
    13561 ttgcatcgac aacgctcgca tgtcggcggc ttggtgtgtc tgtctcgaga cactttcggt
    13621 caacgagcga atcgcgccct gcgtgagcgg caattgtgaa gccgcttttc gcacgagcga
    13681 caatgcagga acagtccgcg ctcgaaagcc agaaggcgca tccacttgga atatcacagc
    13741 cgtcagcatc aacgccgcga aagataccga cacttctacg ccagggaaac agttcgtcct
    13801 cgacgtgggt gtcaaaattc ggctgatgga aagtcttgag ttcgccgtgg tcgacaattt
    13861 ctgcattgaa catgctgggt cttggagcgt aggcagtatg cacgtcggcc tcgtagagtt
    13921 gagctgccac caagtcatag caatcaggca tcgtacctcc gacctgacga gcttcgtcgg
    13981 tgaggggggt gatgctccaa tccgtcttgt tgccgctatt tcgaccgaaa agcctgacct
    14041 taacattcgg aaacgggtta aaaaccctag taggacttgt tttcatgcgt gtctttcgtt
    14101 tttgttgtgg cgagcgctac ccaggcacaa agcctaaaaa ggtagaatcg tctctacccg
    14161 caaattaaca cacgtataga aaaagtcaat gtttagaccg tctacttcaa aagaagttct
    14221 gcaactttat atatgtcccc cgccccgacc gttaccacca caccgtctcg tgaggttgaa
    14281 tcttcaagat cgtctgtaat catttcaaaa gtatcgaggc atctgacgtt ttcattgaga
    14341 gggcggattt tttcggctaa catgcgtgat gaaatgctcg gatcaaactt ttctcgcgcc
    14401 ggaaatatcg gcgcgagaat gacgtgatcg gcgaccgaaa gcgactccgc aaatgcatcg
    14461 agatgatctt tggtgcggga gtagagatgc ggctggaaga cgacggtcaa tgttttgttg
    14521 ggatattttt cccgaactcc ttgtatcaga gccgaaattt tctggggatt gtgagcatag
    14581 tcatcataga ccaaagcccc gcttttcatc ttgcctttgt actcaaatcg tcgcgccgtg
    14641 ccctgaaatg cggccagaaa tattttcacc ttttctcgtt cgataccaag ttcggttgcg
    14701 acatgcaaag ccgcggccgc attacgtctg ttgtgctctc ccgggaacag taaggtgaac
    14761 tcctccagag ccggcgtctc aaaaaagaga attttttgag gcaaaccttc gagtacggcg
    14821 acaacatttc ggttttcttt gttcgcgatg atgatcccct tgagtggcat acgcgcgatc
    14881 agttctttga acgcgctttg tatatcgctc aagtctttaa agtaatcgag gtgatcttca
    14941 tcaataacag tgataaccaa gattgacggg gtgagattaa gaaatcctcg gcgatattca
    15001 tcagcctcaa ccactaagta tttactcttt ccagcaataa aatttgttcc gaaggttcga
    15061 cctttctccc gatctaaggt cgaaccttgt agaagcgagc caacgatgac ggtcggatca
    15121 agtccggcat caatcaatat tttggcaatc atcgcggtcg tggtcgtctt gccgtgcgtg
    15181 cctgcaaccg cgattgtata tttcccattt gaaatttcgt gaagcgcttg cggatatgac
    15241 aatgacgaga tagctaatgc cttaatctgc gcaagaaaat caacagcata cttttcaatc
    15301 gccacagtgt aaatgatgag gtcaattcct tcgggtataa gctcaactcc ctgcccgatc
    15361 gttatctttg ctcccagctt ttggagctct tcagtaattt cggattctga cagatccgag
    15421 ccatgcacct ctttattttc aagcaaaaac atccgagcaa ttgccgaaac tccaatccct
    15481 cctatcccaa taaaataaac agaattaact tttttgaaat caatcatata tgttatgccg
    15541 acttgccccg tagacagcta tgctgttctt cggggtacct cctatcccaa caaagaaaac
    15601 ccgcctcgca tcggcgaagc tctgcgagtc gaggcgggct ttcttagcat tctcaaacat
    15661 gtccaaagtc tacgttaaaa tttcgcataa aaaaagccac ggtgtgagcg cgactgtcag
    15721 gcgaccttac ctcaaaaaac gatcaagagc tgacaacaga taccctgcga accagaggca
    15781 caggaaaata aatccgggaa taagtatcgt gcagagcacg acgaaccaag cgggaatgta
    15841 gcggtgagaa ccaagaacct cgccacaatc gggacaaacg tgagaaacaa tctgaaatag
    15901 tctcataatc tggcatcagt gtatcaaaag aaatttgagg aagcaaaaag ttttggaata
    15961 aaaaagtcgc ccgaatttgg acgacatttg agaactagtt ggtaaacact acggagcaac
    16021 caacctaaac ctgtagaacg ccctttcagg accactgtcg tcctgaatac gcactttctg
    16081 cccttccggc aagtcaattg tcacaagagg agtccaagtg atgaggtccc tcgagaactc
    16141 caccacactc cgaagagttt ccccatatcc ttgtggatca cgcataatca cagtgttacc
    16201 ttccactgta aacggaagac catttgttgt cgcgagcact ccgttttttg cgtacgcagt
    16261 caccccaaca ggtggaggct catttg
//
LOCUS       DATDSU010000122         2883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1122299, whole genome shotgun sequence.
ACCESSION   DATDSU010000122 DATDSU010000000
VERSION     DATDSU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122299"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..576)
                     /locus_tag="VI981_04305"
     CDS             complement(205..576)
                     /locus_tag="VI981_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479547.1"
                     /translation="MKHLLPGPLPEHARNFMRRAGYGEHMGREGQLSYTKRLGSGIYP
                     RLHAYVEDRGGSIQVNLHLDQKSASYEGSHAHSGEYDGPLVEREMARLVAFAHQIKIE
                     PAPPPEESQKPKRGFFGTFWQ"
     gene            714..992
                     /locus_tag="VI981_04310"
     CDS             714..992
                     /locus_tag="VI981_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479548.1"
                     /translation="MFTVYILLSKKTGRHYIGHAESMDDRLARHNRGAVRSTKHGVPW
                     RIVYKEIYSTRAEAYRREQKIKSYKSGILFKKILGLWKDKPSHHGEVA"
     gene            978..1065
                     /locus_tag="VI981_04315"
     tRNA            978..1065
                     /locus_tag="VI981_04315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Ser,seq:gga)
     gene            1248..1859
                     /locus_tag="VI981_04320"
     CDS             1248..1859
                     /locus_tag="VI981_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY4479549.1"
                     /translation="MEYRAVGNRCAVILDSLHLTISHEEILSVLNRKGFTVVSVDIIA
                     IARQCIGASQYRRSARLFEAPAVVDCSSFIKWLFAQCGIWLPRRSIQQRELGETVQVH
                     ELVAGDVVFVSGWIDYYHDNPTNGVGHVGIVTGNGTVIHAANKKSNVVESPLDTFLGD
                     AKFRGARRYIPKGVEVLTFETPTDREVEIPDDIRWIVLQSLQK"
     gene            1950..2231
                     /locus_tag="VI981_04325"
     CDS             1950..2231
                     /locus_tag="VI981_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479550.1"
                     /translation="MPVWFTYIVRCVDGSLYTGISTDVEQRVRRHNDGQGAAYTRSHR
                     PVILVWTEEAESESAARKREAQIKGWSRQQKENLVKGIEENLGQIYERI"
     gene            2218..2709
                     /locus_tag="VI981_04330"
     CDS             2218..2709
                     /locus_tag="VI981_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEY4479551.1"
                     /translation="MKEFKISFEDYREFNDQSLLSILRSMTWKIALPFVALIILGLTY
                     QNNFKMTVWTFVIVTIMIFGTFMYTRKKSLRKIYDSNKAYQESIQVVFDDEGIRWQTV
                     SGNYKIKWQDMYGYRLFKNMTILYQDRIIGHIIPLRAFENDEEKNRFSKLLEKNVPKQ
                     GGN"
BASE COUNT          756 a          629 c          719 g          779 t
ORIGIN      
        1 agaagacgta ccaggcgaag cacccgacgg agtatgggtt cgtcaccacg tatctgggtc
       61 cggacagtat caagtcggta accgacccga tcactggtga accaactgtc gagaaattag
      121 cggacgtcat tccgctctct acgaccgtca aggcgaccta gccgaggcgg ttttttcttt
      181 ctccaaagcc ttggcggcgg cgggttattg ccaaaacgtg ccgaagaagc cgcgtttggg
      241 tttttgagac tcttcaggag gcggtgcagg ctcgatcttg atctgatggg caaaagcaac
      301 caaacgcgcc atctctctct ccacgagcgg tccgtcgtat tcaccgctgt gcgcgtggct
      361 gccttcataa ctcgcggatt tttgatcgag atggagattg acctgaatgc ttcctccgcg
      421 atcttccaca tacgcatgca ggcggggata gatgcctgat cccagacgtt tggtgtaact
      481 gagttgtccc tcacggccca tgtgctcccc gtaaccagcc cggcgcataa aattgcgcgc
      541 atgttccggg agcggacctg gcaaaaggtg tttcatacgc ggcttatcgt accatggaca
      601 ttgatcagga cgcacgctat actttgctca cgaattttag gaattgatgg agaggtcgca
      661 tatcacgcga agcgggaccc cgctttgcgg gggtggcctt gcaaagctcg cgcatgttta
      721 ccgtttatat tctcttaagc aaaaagacgg ggagacatta tatcggtcat gccgaatcca
      781 tggatgacag acttgcgcgt cacaatcgcg gtgctgtccg atccacgaaa catggtgtac
      841 cttggcgcat cgtctataaa gaaatatact caaccagggc agaagcttat agacgcgaac
      901 aaaagataaa gtcttataag agcggtatcc tattcaaaaa aattttaggg ctttggaagg
      961 acaagccgtc tcatcacgga gaggtcgcat agtggcctag tgcgcttgct tggaaagcaa
     1021 gtataccgaa aggtatcgag ggttcgactc cctccctctc cgccagagtt tcattttgaa
     1081 ctacatttat gattcatagt catcgaataa aaacttggtc tagctttatt catttgtttt
     1141 ttgtctcccg gatctagagg gcaggttttt ggtatccgaa actatctgcc ctgctcgggc
     1201 attttttgta gttctttcat acaccaactc ttcaaaggag gacgaaaatg gaatatcgag
     1261 ctgtcggaaa ccgttgcgct gttattcttg attctctaca cctgacgatc tcgcatgaag
     1321 agattttgtc tgtgttgaac cgtaagggct ttactgtagt aagtgtggac atcatcgcga
     1381 tcgcccggca gtgcattggt gcctcgcagt atcgtcgtag cgcacgacta tttgaagctc
     1441 cagcagttgt tgactgttca agcttcatca agtggctttt tgcgcagtgt ggtatctggc
     1501 tgccgcgacg atcgattcaa cagcgtgagc ttggtgaaac tgttcaagtc cacgagcttg
     1561 ttgccgggga tgtggtattt gtctctggct ggattgacta ttaccatgac aatcccacaa
     1621 acggcgtcgg tcatgtaggt attgtaacag gcaatggaac agtgatccac gccgcaaaca
     1681 agaaatctaa cgtggtggaa agtccactcg acacgtttct tggtgatgct aagtttcgtg
     1741 gcgcgagacg ttatattcca aagggcgttg aggttcttac gtttgagact cccactgata
     1801 gggaagttga aattcccgac gacataagat ggatcgtact tcagtcactc caaaagtgat
     1861 tttcagtgtg cgtatattta tctgcactgg cctcagctcg actggggcct cttcttttcc
     1921 gtttcattga ggggtgatac cttaaaatta tgcctgtgtg gtttacatat attgtccgat
     1981 gcgttgacgg aagcctttat acgggtattt caacggatgt tgagcaacgg gtgagacggc
     2041 ataatgatgg tcagggggct gcgtatacaa gaagccacag gcctgtcata ttggtttgga
     2101 cagaggaagc cgagtcagaa tcagccgccc gcaaacgtga agcacaaatt aaaggttggt
     2161 cacgtcaaca gaaggaaaat ctcgtaaagg gaatagaaga aaatctagga caaatctatg
     2221 aaagaattta aaatatcgtt tgaggattat cgagagttta atgatcaatc tctcctttca
     2281 atccttcgtt ccatgacatg gaaaatagct ctcccgttcg ttgctcttat tattttgggt
     2341 ttgacttacc aaaataattt caaaatgacc gtgtggactt ttgtcattgt aacaatcatg
     2401 atttttggca cgttcatgta taccaggaaa aaaagtttac ggaagattta tgactcaaat
     2461 aaagcctatc aagaaagcat acaggttgtt tttgatgacg aggggataag atggcaaacg
     2521 gtttcaggga attacaaaat caaatggcag gatatgtacg gatacagact ttttaaaaat
     2581 atgactatct tgtatcaaga cagaatcatt ggtcacatta tcccgttacg agcctttgaa
     2641 aatgacgaag agaagaaccg tttttccaag cttttggaga agaacgtccc caaacaaggc
     2701 gggaattgaa gccgcttttg acttcttttg agccagtggt aaaaagtgaa ggaggtagct
     2761 gtttttaata cccgtctatg aaaacatccg tcttgctact tatcgttgtc gtattattga
     2821 tccttggcgc cgcctggtat atctggagcg agcagggaaa agataagtcg aacgaataca
     2881 aca
//
LOCUS       DATDSU010000123         3339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1462038, whole genome shotgun sequence.
ACCESSION   DATDSU010000123 DATDSU010000000
VERSION     DATDSU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462038"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1029)
                     /locus_tag="VI981_04335"
     CDS             complement(187..1029)
                     /locus_tag="VI981_04335"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEY4479552.1"
                     /translation="MKIEIKNNAKIIAYVHLPCVDYGLSDNDKNGLLDCLLREIEDSD
                     SGYAGFSTKENLNNYLRGAVFDKADDNNHQYTFTLDEVRIIKTITKAIKKCSDLLPGK
                     ITSIFVFPTFSQFVKEEMSGTTGYTPWPDTILIFINPANPQYDRALSETVGHEFNHAI
                     FLRDKKCESLLDSMIFEGLAERFREQVIGGGQAPWTKIFGLNRAKVIFLEMKLANLLQ
                     LTNPEICRGVFFGNEKYIRWTGYALGYHIVKSFLENNPSLDWKEIMTRQPKDIFSGSS
                     FGNK"
     gene            complement(1035..1223)
                     /locus_tag="VI981_04340"
     CDS             complement(1035..1223)
                     /locus_tag="VI981_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479553.1"
                     /translation="MDILKNLDLLSVGVVIAGIGVLGFTVLLSFLRDPSKEELKSSNI
                     PNWQTKIYVDGYMRIYEG"
     gene            1365..1856
                     /locus_tag="VI981_04345"
     CDS             1365..1856
                     /locus_tag="VI981_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479554.1"
                     /translation="MDKHKLFQQIESSIKEQIADAERARQDSQDEANRHVGRMQSRYD
                     TFKEEAQQMAAAHELRKVGLSRSLSTIRSFLADLDTLQTGGMIRIGSVVLLESLDGQK
                     QKRVALAPVGGGIKISQEVNEVTIVTPSAPLGRTLLGKLGGEKIELILDGKTVTYAIL
                     EVI"
     gene            1856..2413
                     /locus_tag="VI981_04350"
     CDS             1856..2413
                     /locus_tag="VI981_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479555.1"
                     /translation="MNLFISLDGVDGVGKTTVAKLLAADGVFRYYKSPAGPFAQLRKE
                     IDAHATPLERYCFYRLATQFDSVQISRSLETNSVVCDRYIASTAAYHIAMDARIRVIH
                     NDVELLEPHFAFLLGARSEVRDKRILERAKVLSDVKLEGDSTLLDHVAEIFMSFGLIY
                     IDTSDITADEVAPTIKHIVAQGEML"
     gene            2410..>3339
                     /locus_tag="VI981_04355"
     CDS             2410..>3339
                     /locus_tag="VI981_04355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY4479556.1"
                     /translation="MSLKPLSYAHIMAIADAWRDMGGALEVEIGALEPLLWRDEQYRI
                     HDVVGALTGRGFKVSMTTNGQLLDIFAENLSRAGLSLIRSSWHSTSPLMFREISGGYG
                     DYDRFMRGITLALESGIKIAFNRVLHKGYTSDILEQLSFVERYKSRLKLYTLLWTPQS
                     ASTHESFYQDWHPVVRESVLPRTLKIVRVRKQLGRERLRFHLIGGGSVEVKLCDKLNR
                     SMHPCVSCSFKKECEEGFGDYVRVDPRLHLYFCYMRRDLGFQIPEYFGRPEVLKQKIQ
                     EAVRTIDVASLLATAPLRLTVTPFCNFNCRAPGV"
BASE COUNT          977 a          734 c          729 g          899 t
ORIGIN      
        1 aagctactgc aaacaagcaa ccgaagcggt tcatctggtc aatcaaaagc aaaccgcgca
       61 ctagccagat gacgtcgatg aagagcagcg actcttcgag ccagagcgct caaagtaaag
      121 actgagcgcg caccggatga aaccggtcaa aacaaccctt aattgccttc ccaggcaatt
      181 aagggtttat ttattaccaa aactagaccc actaaaaata tctttcggct gtctggtcat
      241 aatttctttc caatccaaac ttgggttgtt ttccaaaaat gatttaacga tatggtaacc
      301 aagagcatat ccggtccaac gaatatattt ttcattacca aaaaatactc ctcgacaaat
      361 ttcaggattg gttaattgta ataagtttgc cagtttcatt tctaaaaaaa taactttcgc
      421 ccgatttaat ccaaagattt tggtccaagg agcttgacca ccaccgataa cttgttcacg
      481 aaaacgttcg gccaacccct caaaaatcat tgagtctaaa agggattcac attttttatc
      541 tctcaaaaaa atagcatgat taaattcatg tccgacagtt tcgcttaaag ccctatcgta
      601 ttgcgggtta gcgggattta tgaaaattaa aatagtatcc ggccaagggg tatagccggt
      661 cgtccccgac atttcctcct taacaaattg actaaatgtt gggaagacaa aaatactagt
      721 aattttccct ggtaaaagat cagagcattt tttaatagct ttcgttatag ttttaattat
      781 ccttacttca tctaaagtaa aagtatattg atggttatta tcatcggcct tatcaaaaac
      841 ggctcctcgc agataattat tcagattttc tttagtgcta aaaccggcat aaccactgtc
      901 tgaatcttca atttccctta ataaacaatc gagtagccca tttttatcat tatcacttaa
      961 gccataatct acacagggca aatggacata agcaattatc ttagcattat tttttatttc
     1021 tattttcatg aattttatcc ttcataaatt ctcatatacc catccacata gattttcgtt
     1081 tgccaattag ggatattgct tgatttcaac tcttctttac ttggatcgcg caaaaaactc
     1141 aacaaaaccg taaaaccaag cacgccgatg ccggcgatga ccacgccgac cgaaagcaaa
     1201 tcaagatttt tcaggatgtc cattggggta cattgtaata gaaactgtga acaaaaacaa
     1261 aatttgacgg taaaagttcc ttgtggtatc tttctataag aaattggctc taaagttgag
     1321 ctatagttac tagcacattg aaaaataaaa atgggagtca aaaaatggac aaacacaagc
     1381 tttttcaaca gatcgagtct agtattaaag aacaaatcgc agatgccgaa agagcgagac
     1441 aagattccca agacgaagca aacagacatg ttgggagaat gcaatcaagg tacgacacct
     1501 ttaaggagga ggctcagcaa atggccgcgg cccatgagtt gcgtaaagta ggattgagca
     1561 gatcattgtc gaccatcaga agtttccttg ccgacctaga cacacttcag actgggggca
     1621 tgattagaat tggaagtgtg gtccttcttg agtctctaga tggacaaaaa caaaaacgtg
     1681 tagcacttgc cccagtcggt ggtggaataa aaatttcaca ggaagtaaac gaggttacta
     1741 ttgtcacgcc atctgctccc ctcggccgaa ctcttttggg aaaattaggt ggtgaaaaaa
     1801 tcgagttgat actcgacgga aaaactgtaa cttatgcgat tttggaggtg atctgatgaa
     1861 tttgtttatc agtcttgacg gagttgatgg tgtcggtaaa accacggtag caaaactcct
     1921 cgccgctgac ggagttttcc gatattacaa atctcccgcc ggacctttcg cacaattgcg
     1981 aaaagaaatt gacgctcacg caactccgct tgagcgatac tgcttctacc gactggcaac
     2041 gcagtttgat tcagtacaga taagccgatc gttggaaaca aactctgttg tctgtgatcg
     2101 ctacattgcg tctacggcgg catatcacat cgcaatggat gctcgcattc gagtcatcca
     2161 taacgatgtt gaacttctcg agccacactt cgcgtttctt ctcggcgctc gttcggaggt
     2221 tcgtgataag cgaatacttg aacgcgcaaa agtgttgagc gatgtaaaac ttgagggaga
     2281 tagcacgctc cttgaccatg tcgctgaaat ctttatgtcg ttcggtctca tctacattga
     2341 caccagcgac atcacggccg atgaagtcgc cccaacaatt aagcacatcg ttgcgcaagg
     2401 agaaatgcta tgagcttgaa gccgctttcg tacgctcaca ttatggccat tgcagatgcc
     2461 tggcgtgata tgggtggtgc tctcgaagtg gaaatcggcg cgcttgaacc gcttttgtgg
     2521 cgggacgagc agtatcgcat tcacgatgtc gtaggtgcgc tgacgggacg tggtttcaag
     2581 gtttcgatga cgaccaacgg gcaactgttg gacatcttcg ccgagaattt gagtcgcgcc
     2641 ggcctctcgc tcatacgatc gagttggcac tcgactagcc cgttgatgtt tcgggagata
     2701 tccggaggct acggcgatta cgatcggttc atgcgcggga taacacttgc gcttgaatcg
     2761 ggtataaaaa tcgctttcaa tcgtgtgctt cacaaagggt acactagtga tattctcgag
     2821 cagttgtcat tcgtcgagcg atacaaaagc cgactcaaac tctacacgct tctgtggaca
     2881 ccacagagtg catctactca cgaatcgttc tatcaagact ggcatcctgt tgtgcgagaa
     2941 tcggttttgc cgcgcacact taagatagtc agagttagaa agcaacttgg ccgtgaacgt
     3001 cttcggtttc atcttattgg aggcggatcg gtcgaagtga agctgtgtga taaattaaat
     3061 cgtagtatgc atccctgcgt atcatgctcg tttaagaaag agtgcgagga ggggttcggt
     3121 gactatgttc gagttgaccc gcgtctgcac ctctatttct gttacatgcg tcgggatcta
     3181 ggattccaaa taccggaata cttcggaaga ccagaggtat tgaaacagaa aatacaagaa
     3241 gcagtcagga caatagatgt tgcgagtctt cttgcaacgg ctccgctacg cctaacagta
     3301 acgccattct gcaattttaa ttgcagagca ccgggggta
//
LOCUS       DATDSU010000124         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1462557, whole genome shotgun sequence.
ACCESSION   DATDSU010000124 DATDSU010000000
VERSION     DATDSU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462557"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VI981_04360"
     CDS             <1..498
                     /locus_tag="VI981_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEY4479557.1"
                     /translation="KPTSVFLLGRYKHNKPESFSSYAHSYKKLSIKYLTIHASKGAEA
                     DYVVILDVISGKHGLPSEITDDPLLEIVLSKGDSYPHAEERRLMYVAMTRARQKVFVV
                     TDAGRESVFVLELEGTKNKKTQMIQCDECGGEMIERKGPYGVFLGCSNFPDCQHKINL
                     RKGRR"
     gene            complement(854..1396)
                     /locus_tag="VI981_04365"
     CDS             complement(854..1396)
                     /locus_tag="VI981_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479558.1"
                     /translation="MTSFSTIKKQFRDLSLGGQDELLKDLYMFSKDVRLFLENRLLRG
                     TTATDEYVRQMERETIKKVYRTGTPGIPNGKTVNSIIAKAKKSHVDIHALMTLEQLAY
                     RGFIEFLNEFGGGPESFDDQSCKHLEAYLQIVRDGQIDGEERARRYQELKTYLLAKNN
                     MLTDSLDEAYESVTGISIGR"
     gene            complement(1421..1786)
                     /locus_tag="VI981_04370"
     CDS             complement(1421..1786)
                     /locus_tag="VI981_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY4479559.1"
                     /translation="MVVKKNNVREQRIIYEIVVDAYGSEEQALGWYYYIENALEFPFH
                     AICISTRANSPLRKEEVVKVIGMPAEKDCTQEVLVTIEFCDHTMSVPLCQLKPLSVAP
                     NARKAIDDWQYWVARGYQF"
     gene            complement(1812..3680)
                     /locus_tag="VI981_04375"
     CDS             complement(1812..3680)
                     /locus_tag="VI981_04375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY4479560.1"
                     /translation="MKTTLKKYFGYDEFRPLQEEIIERILRGHDCVVLMPTGGGKSLC
                     FQLPALMLPGMAIVVSPLISLMKDQVDALRANGIVAEFINSTLSHAEIAGVMEQARNG
                     ELKILYIAPERFGVPGFENFVRTLNISLVAVDEAHCISEWGHDFRPDYRNLKLLRNKF
                     PAITIIALTATATEKVREDIVKQLALKKPQIFISSFNRPNLSYEVLPKKDSLKSILTL
                     LNSYRNESAVIYCFSRNDTEKLVEDLNTHGFTAAAYHAGLPANKRKENQERFIRDEIN
                     IMVATIAFGMGIDKPDVRLVIHHSLPKSIEGYYQETGRAGRDGLPARCALFFSYADKF
                     KHDYFIRNMADETEQKKAQEKLDQVLRYGNLHGCRRLFLLRYFDETYDKSNCGNCDGC
                     VMRTPLHIDKTGLNLRATKPKTSYLSSSVGRNMYNPALFEELKSIRTLEARRLKVPPY
                     IVFGDKALREMATHFPQTSADFLEMSGVGNQKLSKFGELFMSAIRRYAETHNATIQGK
                     TKLHLHNEHRTRSIHAGSTIDETKKFILQKISIDEIAKTRGLSRGTILSHIEKVSNTD
                     MQVDLSYVKPPQERFEKIAAAFKKTGGFALTPVRGILGDGYSYDELRLARIFLKRL"
     gene            3908..4120
                     /locus_tag="VI981_04380"
     CDS             3908..4120
                     /locus_tag="VI981_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479561.1"
                     /translation="MKREVLIKFGQKVRERRFELGLSQEELAARAGVHRTYVGMIERA
                     EKNITLTNIEKIAKALGLSLDEITKL"
BASE COUNT         1130 a          918 c          937 g         1201 t
ORIGIN      
        1 aaaccgacca gtgtttttct tcttggacgg tacaagcaca acaaaccaga gagtttttct
       61 tcatacgcgc actcatacaa aaaactgtct atcaagtacc tcacgattca tgcatctaag
      121 ggcgcagaag ccgattacgt ggttatcctc gatgtgatct cagggaaaca cggactgccc
      181 tcagagataa ccgacgatcc gttgttggaa atagttttga gcaagggaga ttcatatcct
      241 cacgcagagg agcgtcgcct aatgtacgtg gcaatgacga gggcgcggca gaaggtattt
      301 gtcgtcaccg acgcgggcag ggagtcagtg tttgtccttg agctagaggg cacgaagaat
      361 aaaaagacgc agatgatcca atgtgacgaa tgtggtggtg aaatgattga aagaaaaggt
      421 ccatacgggg tgtttcttgg ctgtagcaat ttccctgatt gtcagcacaa gattaatctc
      481 cgaaagggtc gtaggtaagc gttgggcgca ggcgtaaatt cggaaatgac acccacttga
      541 ggatgcagac ctaatgggta cttgttccac cccatggggg gtcatttcgg cccatggcgg
      601 gggtggggct aggctttttc tagctcaggc acataaaacc attgtagatt cgcctctaca
      661 tatgttctaa tagcttcaac ggttcgccgc agagaatacc ggttatcggc gagacccgag
      721 aaccttatgt tgagccattt tttgaaatcg atttttacaa aaaattgatc gggaggatgg
      781 gattcgaact tttgaggaca aaaagctttt tatcgggaga cccgtccttc tttctcgacc
      841 acgttgctcg agctcaacgc ccgatgctta tgccagtaac tgattcgtac gcctcatcaa
      901 gcgagtcggt gagcatattg ttttttgcaa gcagatatgt tttgagttct tggtatcgtc
      961 gcgcacgttc ttctccgtca atctgtccgt cccgcacaat ctgtagatat gcttcaaggt
     1021 gtttgcaaga ttggtcgtca aaactctcag gccctccgcc gaattcattt aaaaactcga
     1081 taaacccgcg ataagcaagc tgctcgaggg tcatcagcgc atgaatgtcc acatgagact
     1141 ttttcgcttt cgcaataatg gagtttaccg ttttcccgtt tggtataccc ggcgtgccgg
     1201 tccgataaac cttcttgatg gtttcgcgct ccatttgtcg gacatactca tcggttgcgg
     1261 ttgtgcctct aagcaatcga ttttccaaga acaacctcac gtccttcgaa aacatataga
     1321 gatccttcaa taattcatct tgaccaccaa gagagagatc tcgaaactgt ttcttgattg
     1381 tagaaaatga tgtcatattc tatgggcgtg ttaattttga ttaaaattgg tatccgcgcg
     1441 ccacccagta ctgccagtcg tcaattgcct tacgcgcgtt tggagctacc gagagtggct
     1501 tgagctgaca gagcggcact gacatggtgt gatcacaaaa ctcaatagtc accagtacct
     1561 cttgggtaca gtctttttcg gcgggcatac cgatcacttt taccacctct tcttttcgaa
     1621 gtggcgaatt tgcccgtgtg gaaatgcata ttgcgtgaaa aggaaattcg agcgcatttt
     1681 cgatgtagta gtaccaccca agtgcttgtt cttcactccc gtatgcatca acgacaattt
     1741 catagatgat gcgctgttca cgaacattgt ttttcttaac gaccatattg cctatttata
     1801 gacatctgtt tttataaacg tttgagaaaa attctggcca atctcaattc gtcatacgag
     1861 taaccatcgc ccaagatacc gcgtacagga gttagggcga aaccgcctgt ttttttaaag
     1921 gccgctgcga ttttttcgaa tcgttcctgt gggggtttta catatgaaag atcgacttgc
     1981 atatcagtgt tagaaacttt ttcgatgtga ctaagaatag tgcctcgaga taatccgcgc
     2041 gttttagcta tttcgtcaat cgaaatcttc tgtaatataa attttttagt ttcatcaatc
     2101 gttgaaccag cgtggatcga ccttgttctg tgttcgttat gtaaatgcaa ttttgttttc
     2161 ccttgaattg tcgcgttatg agtttccgcg taacgacgaa tggcggacat gaatagttca
     2221 ccaaattttg agagtttttg atttccaaca ccgctcattt ccaaaaagtc ggctgatgtt
     2281 tgtgggaaat gagtggccat ctcgcggaga gccttgtctc caaaaacaat atatggcggg
     2341 acttttaatc tgcgggcttc cagtgttctt atgcttttta attcctcaaa caatgcaggg
     2401 ttgtacatgt tccttccgac agatgatgac agataagaag tttttggttt agtcgccctg
     2461 agattcagac ccgttttgtc tatgtggagc ggagttcgca taacacatcc atcgcagttt
     2521 ccgcagttac ttttgtcata ggtttcatca aaatagcgaa gcaaaaacag gcggcggcat
     2581 ccgtgaagat ttccgtagcg caagacctga tccagcttct cttgcgcttt cttttgctcc
     2641 gtttcatccg ccatgttgcg tataaaatag tcatgcttaa acttgtcggc atatgagaaa
     2701 aacagggcgc atctcgccgg caggccatcg cgtcccgctc gaccagtttc ctgataatac
     2761 ccctctattg attttggcaa gctgtgatga ataacaagcc gtacatcagg cttgtcaatt
     2821 cccatgccaa aagcgatggt cgcaaccata atattgattt cgtctcgaat aaatcgttcc
     2881 tgattttcct ttcttttgtt tgcagggagc cctgcgtgat aagcggcggc cgtgaatcca
     2941 tgcgtattta agtcttcaac cagtttttcc gtatcgttgc gcgaaaaaca atagatcact
     3001 gcgctttcgt tgcggtaact atttaaaagc gtgagaattg attttaaaga atctttcttt
     3061 ggtaaaactt cataactcaa attaggtcgg ttgaaactgg agataaaaat ttgaggtttt
     3121 tttaatgcga gctgtttgac aatatcctcg cgcacttttt ctgtggcggt agccgtcaga
     3181 gcgataatcg tgatcgcggg aaatttattt cgcaataatt ttaaatttcg atagtctgga
     3241 cgaaagtcgt gtccccactc tgaaatacag tgggcttcat caacagcaac taagctaatg
     3301 ttcaacgtac gcacaaaatt ctcaaaacca ggaacaccaa atcgttctgg ggcaatatag
     3361 agaattttca gttctccgtt ccttgcttgt tccatcacac ccgcgatttc agcatgcgaa
     3421 agcgtgctgt taataaactc agctacaatt ccgtttgcac gaagggcatc aacctgatct
     3481 ttcattaaag aaatgagcgg cgaaacaaca atcgccatgc cagggagcat aagtgcagga
     3541 agctgaaaac aaagagactt tcccccgccc gtaggcatta aaacgacaca gtcatggcct
     3601 cgcaatatcc gttcaataat ttcctcctgc aacggcctaa attcatcata cccaaaatat
     3661 tttttaagcg tggttttcat aaaagtgtat tttttatccc ttgaccgaga agtcgcccaa
     3721 tattgaacga cacccatggt tattcttgtg cttcaaaccc ttctttgcgg taaaaggatc
     3781 ggcgcctctt ccacattttt tcaaacagtg gtacttgcag gtctcgatag ccaaatatac
     3841 atatgtatgt gctaactata gtatgtagaa ccaaagttag caagagcgta gactatagtt
     3901 atcaattatg aaaagagagg ttttaataaa atttggtcaa aaagttcgtg agcgacgctt
     3961 cgagcttgga ttgtcgcagg aggaattagc ggcaagagcg ggtgtacatc gcacctatgt
     4021 tggcatgata gagcgagcag aaaagaatat caccttaacg aatatcgaaa aaattgcaaa
     4081 agctctcggt ctttcactgg acgaaatcac gaaattataa atatgggaac aaacgaatta
     4141 agaaaaagaa aaatttggca ggatgtcagc ggtggaaagg ccggag
//
LOCUS       DATDSU010000125         4128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1126878, whole genome shotgun sequence.
ACCESSION   DATDSU010000125 DATDSU010000000
VERSION     DATDSU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126878"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..143
                     /locus_tag="VI981_04385"
     tRNA            68..143
                     /locus_tag="VI981_04385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101..103,aa:Thr,seq:ggt)
     gene            complement(377..688)
                     /locus_tag="VI981_04390"
     CDS             complement(377..688)
                     /locus_tag="VI981_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479562.1"
                     /translation="MPRTLHTGGGVGSPVVGYPCRLRYAESMGRPTLRYARQRGAELE
                     THRVGGRSRIARAGSGAIPRPRQTYHYICSGGHRAERLPRTASFAHVREEVCRGANSP
                     S"
     gene            1691..1897
                     /locus_tag="VI981_04395"
     CDS             1691..1897
                     /locus_tag="VI981_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479563.1"
                     /translation="MKDEVRKLAENLKRIRAEKGISQGDIVKSTGIDKAMISNIENGK
                     TNPTLTTIAKLAKAIGVSVGELMK"
     gene            1902..>4128
                     /locus_tag="VI981_04400"
     CDS             1902..>4128
                     /locus_tag="VI981_04400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY4479564.1"
                     /translation="MFLTDNEKRDIAKYIEAGKLLPDKYRFLLFEGKQEVELVWNGKT
                     DEITNTVLPFQTIEQVDEPRSEAVAKLQGSLFGTGGRQISGWTNKLIWGDNKLILSSL
                     KNGALREEIEAQGGIKLIYIDPPFDVGADFSMNIEIGDEELTKKPNVLEELAYRDTWG
                     KGADSFISMIYERLSLMKDLLAEDGNIYVHCDWRVSGYMRLVLDEVFGKGNFRNEIIW
                     QGAVGDTSAKNKKFIKSHDSIFLYTKNSDSAVWNDVFQEYSEASNNLYRFNDEKGNFR
                     LAPIDNPGGKGYIYDLGFGEKMPANGYRMPKETALKWLAEGILEIKAGRVPGRKLYKN
                     EEGVRCRDVWTDIRSLQGSETIGYATQKPESLLERIVKASSNENDLIADFFCGSGTTF
                     AVAEKLGRKWLGSDLGKFGIHTTRKRMIGVQRQLKKENKDYRAFEILNLGKYERQYYV
                     GINPNLREEEKQKQIAQKEKDFVSLILSAYKAEAVEGFKTFFGKKNSRLVSIGPINLP
                     VSRLFVEEVIAECIEKKITKADILAFEFEMGLFPHIQEEAKDKGVDLALKYIPREVFD
                     KRAIEKNQVVFHDVSYIEVKPIIKKNQIAIELTDFSVFYNQDSVKEVEENLKNGGKKL
                     VVENGQIIKIAKDKEGITTREVLTKKWTDWIDYWAVDFNFESKKEVIRVKNEETGEFE
                     DRWTGDYIFENEWQDFRTKKKRDLQLTTPPVEVSPGKRKVAVKVVDIFGNDTMKVVEI
                     KI"
BASE COUNT         1150 a          833 c         1068 g         1077 t
ORIGIN      
        1 catgcaacca agtataccat tctggcaata tgtagtccgt gttattatcc ctaagccgtg
       61 tttgtgcgcc gatgtagctc agttggtaga gcacgtcatt ggtaatgacg aggtctccgg
      121 ttcaatcccg gacatcggct ccaagattaa agtagtagaa atttttccaa ttttttcttt
      181 ggcggtagcc aaagaaaacc attgatttac cgcaattccg cacgcttcgc gtgcttttag
      241 ggtgaggttc gagccgaaga ttttttggag ggaggatttt ttggagggga ggtcgtctgt
      301 atttcgtatt tcgtccagta ttgaagcgtc tttaatccag tctcgcgtcg gttcgatcca
      361 cgcggagggg gtatgttcaa gacgggctat ttgctcctcg acagacttcc tctcggacat
      421 gagcaaacga cgccgttctc ggtagtcgtt ctgctcgatg tcctccgcta catatatagt
      481 ggtaagtctg gcgaggtcgc gggatagctc cgctacctgc ccgcgcaatc cttgaacggc
      541 ctccgacgcg gtgtgtttcg agttctgctc ctctttgtcg agcataacgg agagttggtc
      601 tgcccatgct ttcggcatag cgtagtcggc aaggatagcc gacaactggc gatccaactc
      661 ctccgcccgt atgcaaggtt cggggcattt ctgcgtcttg gacttcttgg tgcaatgata
      721 ataaacgtag cggtgggttt taccgctttt ctgcctcttt atccgtatct cgccggtaat
      781 acccataccg cacgttccgc accgcagaag cccacagaat ggcctcgggt cggtcttggt
      841 ggagggtaga cggcctcggc ttcgcaggac gacgttcgcc ctatcccaaa ggtctttcgt
      901 tatgatcggt tcgtgcttac cttcgtgtat ctctccggca tagcggaagt gtccgtagta
      961 gaacggattt tgaagaatga aagacattct cgaaatatgg acatggtttt tgtttttgga
     1021 ttggatacct gcctgttcta aaatctcgga gagattttca aggcgaacag ttcctgtcgc
     1081 gtaaagctca aagatttttc tgatgagttt tgcattcttg cggtgaacta caactgtctt
     1141 ggtgcgagga tcgtttatat atccgagcgg agcaaggccg ggatagatac cctgccgcac
     1201 tttttgtcta agtccgcgag cggtgttcgc gcttagttgt cggatatatt cgttcgccat
     1261 tcctaactcg acggacatca ttaaaacatt gtcgtttgga taatggcttc tgtcgtgcgt
     1321 ttgtatgtgg cgtatcacgc cctgttgcag aagccattgt atctgtccgc cgtccacggg
     1381 gttgcgagcc aagcggtcaa tcttccaaca gataatcccc tgtgcctcgc cccgctctat
     1441 gcgacgtagc atatcgttaa agacggggcg tccgggtttc ttcgcggagc gtttctcgat
     1501 aaactcctcc gcgattggga gattttcacg cttggcgagg tgtcgcaatt cggcgagttg
     1561 gccttcaatg gactggacct gtttgtcctc aacatcggta ctttttcggg cgtagagaaa
     1621 gtatttaggc atggttgtat tgtatcacaa tggatagcat aaaacacaac gggggtttat
     1681 actgtgctta atgaaagacg aagtacggaa actagccgaa aatctgaaac ggatcagagc
     1741 cgaaaaaggc atttcgcaag gtgatattgt taaatctacc ggaattgata aagcgatgat
     1801 tagcaatatt gaaaacggaa agacgaatcc cacgcttacg acgattgcga agttagcaaa
     1861 agcaatcgga gtgtcagttg gagagttaat gaaataaaac tatgttttta accgacaacg
     1921 aaaagcggga tattgcgaaa tacattgaag cgggtaaact cctgccggat aagtatcgct
     1981 ttctcctatt cgagggaaag caggaggtag agcttgtatg gaatggaaag acagacgaga
     2041 tcacaaatac cgtcctgcct tttcaaacga ttgaacaggt agatgagcct cgctcggaag
     2101 cagttgcgaa attacagggt tcgcttttcg gcacaggggg acggcagatt tcgggctgga
     2161 cgaacaagct catctgggga gataacaagc tcattctttc gtctctaaaa aacggggcgt
     2221 tgcgagaaga aatagaagca caaggaggaa ttaaacttat ttatattgat cctccgttcg
     2281 atgtcggagc ggacttctca atgaatattg agataggcga cgaggaactc acgaaaaaac
     2341 caaacgtgct agaggagctt gcctatcgag acacttgggg caaaggagca gacagtttta
     2401 tttccatgat ttatgaacgt ctttctctaa tgaaagacct actcgcagag gacgggaata
     2461 tatatgttca ttgtgattgg agagtgagtg gatatatgcg tctagtttta gatgaagttt
     2521 ttggtaaagg caatttccga aatgagataa tttggcaagg agcagtcggc gacacctcgg
     2581 caaaaaataa aaagtttatt aagtcccacg attcaatatt cttgtataca aaaaactctg
     2641 attccgccgt gtggaacgat gtttttcaag aatatagcga ggccagtaat aatctttatc
     2701 gtttcaatga tgagaagggc aattttcgcc ttgcccccat agataacccc ggcgggaaag
     2761 gctacatata cgatttagga tttggtgaga aaatgcccgc taatggctat cgaatgccaa
     2821 aagagacagc gttaaaatgg cttgcggaag gaatacttga aattaaagct ggccgtgttc
     2881 cgggcaggaa actttataaa aatgaagaag gtgtcaggtg tagagatgtt tggactgata
     2941 ttcgttctct gcaaggtagt gagactattg gatatgcgac gcaaaaaccc gaaagccttt
     3001 tggagagaat agtaaaagct tcgtctaacg aaaatgatct tatagcagac ttcttctgcg
     3061 gctctgggac aacttttgct gtcgcagaaa aattagggcg aaaatggctc ggctctgacc
     3121 ttggaaagtt tggaatacat acaactcgca agagaatgat tggtgtgcaa cgacagttga
     3181 aaaaagagaa taaagattac cgtgcttttg agatattgaa cctcggtaaa tatgagaggc
     3241 agtattatgt cggcataaac ccgaatcttc gggaagaaga aaagcaaaag cagattgcac
     3301 aaaaagaaaa agattttgtg tctctgattt tgtccgcata taaagcggaa gcggttgaag
     3361 gtttcaagac gttttttggg aagaaaaact ctcgcctcgt ttctattggg ccaatcaatt
     3421 tgcccgtgtc gcgcttgttt gtcgaagaag tcattgcgga gtgtatagaa aagaaaatca
     3481 cgaaagccga tatcctcgcc tttgagtttg aaatgggctt attcccgcac atacaggaag
     3541 aagccaaaga caaaggcgtt gaccttgcgc tcaaatacat cccccgcgag gtatttgata
     3601 agagggcaat tgagaaaaac caagtcgtct tccacgatgt ttcctacatt gaagtaaagc
     3661 cgataataaa gaagaatcag atagcgattg agctaaccga tttctcggtt ttctacaatc
     3721 aagattcggt caaagaggtt gaagaaaacc taaagaacgg cggcaagaaa ctcgtcgttg
     3781 agaacgggca gattatcaaa atcgcaaagg acaaagaagg cattactacc cgtgaagtcc
     3841 taacgaagaa gtggacggac tggatagact attgggcggt ggacttcaat tttgaaagta
     3901 agaaagaagt tatacgcgtg aaaaacgagg agacgggaga gttcgaggat cgctggactg
     3961 gcgattatat atttgagaat gagtggcagg acttccgcac gaaaaagaaa agggacttgc
     4021 aactgactac accgccggtg gaggtaagtc ccggcaagcg aaaggttgcc gtgaaagtcg
     4081 ttgatatatt cggcaatgac acaatgaagg ttgtggagat aaaaatat
//
LOCUS       DATDSU010000126         6384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1126943, whole genome shotgun sequence.
ACCESSION   DATDSU010000126 DATDSU010000000
VERSION     DATDSU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126943"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..2023
                     /locus_tag="VI981_04405"
     CDS             473..2023
                     /locus_tag="VI981_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY4479565.1"
                     /translation="MQSIKFFYVSDDLSGYIKAELAKKTSDSEIISFLSDKGWSVGEI
                     HNALSVHRGEKTRAAHKTFKKFVLWEIPLWFSFFMVVFCSAVAAGTVILIGKENIYSY
                     VLTDPEATHENNYLTYGEQPSLSDPDYFGKVRQQFIDEKIDFIEANLDTMKLFVYEDG
                     QPSIEVPILAKGKDGAWWETPAGLYKVESKERNHYSSFGNVYQPWSMRFQGNFYIHGW
                     PYYKDGTPVSSQFSGGCIRLSDESAKRVYDSVKTGTPVLVYERYFAPDSFVYKEKKPA
                     VSAQNYLAADLRNNFVFVEKDAEALLPIASITKLISALVTTEHYNLDSTLTINEDMII
                     KTSKPRLKPGMNLGIYELLKLMLVESSNEAALALAEYRGEKSFVSLMNEKARALGLTH
                     TFFVDSYGGGDENVSSAEELFSLAKYLYNNRSFILALTADKLARSPYEAAKFKDIANF
                     NYFASRADFLGGKKGQTTAAHETMVALFEVEISGEKRPIAIIVLQSEDSDGDVEKILQ
                     YIKNRFRE"
     gene            2059..2895
                     /locus_tag="VI981_04410"
     CDS             2059..2895
                     /locus_tag="VI981_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479566.1"
                     /translation="MKLAKITKRAIMTTMKTHKKGYKALYLLIILSLLTPAFASASHS
                     WGGYHWYRSINPLSLNLGDNVSSQWDAYLGEASSDWSVSEVIDTTVIKGGTTPRICSA
                     KTGKVEICNASYGNNGWLGIATIWINGLHITKGTVKVNDTYFSKSYYNKPEWKRLVMC
                     QEVGHIFGLSHQDENNYNTPLGSCMDYSSNPVPNQHPNAHDYEQLLSIYSHLDAVLGA
                     TSQQSLFGRYAAMNAGDLNVNDLQNEIKELRDQKISSMERKLGSGEKVVTFIYWADNS
                     DN"
     gene            complement(2970..4187)
                     /locus_tag="VI981_04415"
     CDS             complement(2970..4187)
                     /locus_tag="VI981_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479567.1"
                     /translation="MTPKENYTKDAFWKWYTIIVMIMAIMIAAAFFLIYRARSETSDD
                     NADSVAIAMQKLTKNDNFRYIESPMVYKDALSYGWEQWSWYASVNTNWKSSHGTEIKT
                     VISEPWGGVQFASPRLSAREYNSLEFDYSQNGSSHVYVSFVSKEGAELGRQPVSWYVI
                     REGERQHVVLPLRNFNAQTTLSGFSLLSDGPIEIVIDNIRFSTTINDKPMWQPPPAAV
                     EIPTETFLPIILPYIHNFSGLGNWKTQTGKISLGQDHTLILEPNTAESTALVLLGGSE
                     NWRDYEFTTTIDWGQGESVSLIARAVSPDNYVSCLFGYNANSAAIYVQKFNQNSAVAQ
                     SPEFPVPIEMAWKDVRIGVRAVGSTIECLINGESVLRAQIDKPQSGGVGIETWDRQGH
                     RVVIKDIRVNLKD"
     gene            4278..5033
                     /locus_tag="VI981_04420"
     CDS             4278..5033
                     /locus_tag="VI981_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479568.1"
                     /translation="MKKLSRNLYFKLLTVCFAFVLFLAILFLFPSREHSLKPALLQHS
                     DSAETKNEVQQRTIPRAGFKPAVTTLFWVGEPSNAENAYIPNDKSAWDSFWQEHYGGV
                     DDPNGRCDFYPCTFIPKENPFYVALPYTDYSADDNFKKSVEQIPWYMPLPIGESYLKN
                     RWVEIEYNGRVCFGQWEDTGPNEDDDFGYVFGNSTIPKNTFGARAGLDVSPALWTCLG
                     LAENEVTYWKFINQTDVPNGPWKRIVTTRSVSH"
     gene            complement(5035..>6384)
                     /locus_tag="VI981_04425"
     CDS             complement(5035..>6384)
                     /locus_tag="VI981_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4479569.1"
                     /translation="IREASKRYTSGELETHYISQRISTKSVWPEAVYTLSAAFILTCL
                     YIFYFAVLILVSLRIVCMFLLLGFSKIKDARRISTHRYNDGVSVLIPAYNEEENIEST
                     IKSVVFNDYQKKEIIVVDDGSTDSTARIVAHLAVVYSGIKLIRTPNNGKATAIRIGME
                     VSTFDVCVIMDADTIFKKNTISKLVARFWDRKVGAVAGKIATARSRSFIDVLQRIEYI
                     VGQNIDKMAFGGVNAINVVPGPVGAWRKSAVRSVGGPSQDTLVEDQDLTLALLEAGYR
                     VEYEPRAIAFTETPHTFRDFLKQRFRWIYGSIQCVWKYKRSLIRKPFSSLSLIIIPNT
                     LIFGFLIPLFSPLIDITLVASVMLGQSEHVIMPYIVFTALDFIYALFAFIPEKKDRLK
                     VFLIPIQRLYYRQVMYYVVLVGIIRALEGTGALWGKVRKIGESQKLYLTDEHQLDNV"
BASE COUNT         1780 a         1421 c         1386 g         1797 t
ORIGIN      
        1 gttacattcc acttttgacg gtggtcagct acccgatgcg ttgggaatcc cgcgatcatt
       61 actgacgcgg atacactggc atttcagcca actcgagcgc gcgctcgact ctcgattcgc
      121 aattacgcag ccccccattt gggacagcct catcgtcgaa atcgtgctca cttgtgttgg
      181 cttacgccta cacatgtatg catagaggtc cctcgacccc tgaaagttcg caaggattgc
      241 tccccgctgg ctgtcaggtt taaggctgat ccttattcgg acttggtccg aaaagaggaa
      301 tcgctgttgc tttctgttca caagttaccg gctagtattg atattactcc atagatcgca
      361 gacgtcaatt gactgtataa tacattctat tgtgtgatga atagaaatgt atcgattaga
      421 cgtttatgaa ttttgtaatt cgtttctcca gctaaagcag tagtatataa ttttgcaatc
      481 aattaaattt ttctacgtgt cagatgattt aagcggatat atcaaagcag agcttgcaaa
      541 gaaaacaagt gattccgaaa tcatcagttt tttaagcgac aaagggtgga gtgtgggtga
      601 aattcacaat gcgctctccg ttcatcgagg tgagaaaaca cgcgccgctc acaaaacttt
      661 caagaaattt gttctttggg aaattccgct gtggttttct ttttttatgg ttgtattttg
      721 ctccgcagtt gccgccggta cggtcatcct tataggtaag gaaaatattt attcatatgt
      781 tctcactgat ccggaggcga ctcatgaaaa taattattta acgtacggcg aacagccgag
      841 tctttccgac cctgattatt tcggaaaagt cagacagcaa tttatagatg agaaaattga
      901 ttttatcgaa gcaaatctcg acaccatgaa actttttgtc tacgaggacg gccagccttc
      961 aattgaggtt cctatattgg ctaagggaaa ggatggcgcc tggtgggaga cgccggctgg
     1021 cctttacaaa gttgaatcca aagagcggaa tcactattcg tcgtttggga acgtgtacca
     1081 gccgtggagt atgagatttc agggtaactt ttatattcac ggatggccct attacaagga
     1141 cggcacgccg gtaagctcgc agttttctgg tggatgcatc aggctttccg atgagtctgc
     1201 caaacgcgtt tatgatagtg taaaaacggg gacgccagtg cttgtttatg agcgatattt
     1261 cgcgccggat tcgtttgtct ataaggagaa aaaaccggct gtctcggcgc aaaactattt
     1321 ggccgccgac ttgagaaaca actttgtgtt tgtcgagaaa gacgcagaag cactgttgcc
     1381 tattgcctca atcacgaaac taatttccgc cctcgtcacc actgaacact ataatttaga
     1441 ctcaacactg accattaacg aagatatgat tattaaaaca tccaagccgc gtttaaaacc
     1501 gggcatgaat ctcggcattt atgaattgct caaattaatg ctcgttgaat cttcaaatga
     1561 agcggcactc gcacttgcgg agtatcgagg cgaaaaaagt tttgtaagcc tcatgaacga
     1621 aaaagcaagg gcgctcggac tgactcatac gttttttgtg gattcatacg gaggcggaga
     1681 tgaaaatgtt tcatcggcgg aagagttgtt ttcgcttgcg aagtatctat acaataatcg
     1741 cagctttata ctggcactga cggcagataa acttgctcgc tcgccatacg aggctgcaaa
     1801 gttcaaagat atcgcgaatt ttaattattt tgcgtctcga gctgattttc tcggcggcaa
     1861 aaagggacag acgacagctg cgcacgaaac gatggtcgca ctttttgaag tggaaattag
     1921 cggtgaaaaa agacctattg caattattgt tcttcaatcc gaggactcgg atggtgatgt
     1981 tgaaaaaata ctccagtata taaaaaacag atttagagag tagtgcgagt agcgttatgg
     2041 agttatacta aattcattat gaagcttgcg aaaatcacaa aaagagctat tatgaccact
     2101 atgaagacac ataaaaaggg ctataaagca ttgtatttac taattatttt atcacttctt
     2161 acgccggcat ttgcaagtgc gtctcactct tggggcgggt atcattggta tcgttctata
     2221 aatcctctta gcttgaatct cggcgataac gtctcatctc aatgggacgc atatttgggt
     2281 gaagcttcta gcgattggag tgtatcagag gtgattgata caacggttat caaaggtggt
     2341 acaactcccc gcatctgctc ggccaagaca ggcaaggttg agatttgtaa tgcatcatat
     2401 ggaaataatg gatggctagg gattgccact atctggataa acggacttca tataaccaag
     2461 ggaacagtaa aagtgaatga cacatatttt agcaagagtt actacaataa gcctgaatgg
     2521 aagcgactag taatgtgtca ggaagttggt cacatctttg gactctctca tcaggacgag
     2581 aataactaca atacaccgct cggctcatgc atggattact cttcaaaccc agttcccaat
     2641 cagcatccta acgcccatga ctacgagcaa cttttgtcaa tttactctca tctcgatgca
     2701 gtactaggcg caacttcaca gcaatcactc ttcggaagat acgccgctat gaacgctgga
     2761 gatctgaatg ttaatgactt gcaaaatgaa atcaaagaac ttagagacca gaaaatttca
     2821 tcaatggagc gaaagcttgg aagcggtgaa aaagttgtca cattcatata ttgggctgac
     2881 aactcggata attaatttct tcttttagac tgacttttcg gaggtaatca caaattggat
     2941 aaacaccggc ctcaagaggc cggtgttttt taatccttca gattgactcg gatgtcctta
     3001 atgacgactc tgtgaccctg ccgatcccat gtttctatac cgactccgcc gctttgcggt
     3061 ttatctattt gtgctcgcag taccgactcg ccgtttatca aacactcaat cgtgcttccg
     3121 actgctcgaa ccccgatcct gacgtctttc catgccattt caatgggcac tggaaattca
     3181 ggtgattgtg caactgcact gttctggtta aatttctgga catagatggc cgcactattc
     3241 gcgttatacc caaacaaaca cgagacatag ttatccggtg ataccgctcg cgcaataaga
     3301 ctcactgatt ctccctgtcc ccagtcaatc gttgttgtaa attcataatc cctccagttt
     3361 tctgaaccgc caagtaaaac aagtgcagtg gattcagctg tattgggttc taaaattagt
     3421 gtgtgatctt ggccgagcga aatttttcct gtctgtgttt tccaattgcc taatccgctg
     3481 aagttgtgta tatacggcaa aataatcggc aagaaagtct cggttggaat ttctacagct
     3541 gccggtggag gctgccacat tggtttatca tttattgtcg tactgaagcg gatgttatca
     3601 ataacaatct cgattggtcc atctgacaga agtgaaaaac cggatagcgt tgtctgtgcg
     3661 ttaaaatttc taagcggcaa aaccacatgt tggcgctccc cctctctaat aacgtaccag
     3721 ctgactggct gacgtccgag ttcggctccc tcctttgaga caaatgatac gtagacatgt
     3781 gacgaaccgt tttgcgaata gtcgaactca agcgaattgt attctcgagc agacagtcgc
     3841 ggtgacgcaa attgcacgcc tccccacggt tcggatataa cagttttaat ttcagtgccg
     3901 tgtgatgact tccaattagt gtttaccgat gcataccatg accattgctc ccagccatat
     3961 gacagcgcat ctttgtacac cattggcgac tctatatatc taaaattgtc attttttgtc
     4021 agcttttgca ttgcaatggc gacggagtct gcattatcgt cgctagtttc cgatcgagca
     4081 cgatatatta gaaaaaaggc ggctgcaatc ataatcgcca tgatcattac aatgattgta
     4141 taccacttcc agaatgcgtc ttttgtgtag ttttccttgg gagtcatact gtggagatgt
     4201 tagtcacaat cttgtctttc ttatataatt aattttcttc agaacgctca tatatttaga
     4261 gatgcatgta taatcagatg aaaaagctct cgcgaaatct gtattttaaa cttttgacgg
     4321 tgtgttttgc attcgtactc tttttggcaa tattattttt attcccgtcc agagaacact
     4381 cacttaaacc tgcactgctt caacattcgg attcagccga aactaaaaac gaggtgcagc
     4441 aaagaacaat cccacgtgcc ggttttaaac ctgcagttac gactttattt tgggtcggag
     4501 aaccatctaa cgccgaaaat gcctacatcc caaatgacaa aagcgcttgg gattcatttt
     4561 ggcaagaaca ttatggagga gttgacgatc cgaatggtag gtgcgatttc tatccgtgca
     4621 catttattcc aaaggaaaat ccgttctacg tagctctacc atataccgac tattccgccg
     4681 atgataattt taagaagagc gtcgaacaaa ttccctggta tatgccgttg ccgattggcg
     4741 agtcatatct caaaaaccga tgggttgaaa tagagtataa cgggagagtt tgtttcgggc
     4801 aatgggaaga cacaggcccc aatgaggatg acgattttgg atacgtattc ggtaactcga
     4861 cgattcctaa aaatactttc ggtgctcgcg cgggacttga tgtttcgccg gcactttgga
     4921 catgtcttgg actagcggaa aatgaagtta catactggaa attcatcaac caaacagacg
     4981 tgccgaacgg cccgtggaag cgcatagtca ccacacgcag tgtctctcac tgaattacac
     5041 attatcgagc tgatgctcgt cagtgagata gagtttctgt gattctccaa tcttgcgaac
     5101 cttaccccaa agtgcccccg ttccctcaag cgcacgaatt attccgacga gaacaacgta
     5161 atacatgacc tgcctataat acagacgctg gatcggtatg aggaatactt ttaagcgatc
     5221 cttcttttct ggaataaacg cgaataatgc atatataaaa tcgagagccg taaacacaat
     5281 atacggcatg atgacatgct ccgattgacc gagcataacg ctcgcgacga gcgtaatatc
     5341 aatgagggga gaaaaaagcg gtatcaagaa tccaaagatg agagtgttcg gtataattat
     5401 aagagaaaga gacgaaaacg gtttgcgtat gagcgaccgc ttatacttcc agacgcattg
     5461 gatacttcca tatatccacc ggaaccgctg tttcaaaaaa tcgcggaatg tatgcggtgt
     5521 ttcggtgaac gcaatcgccc gtggttcata ttcgactcta taccctgctt caagaagcgc
     5581 gagcgtcaaa tcttgatctt ccacaagcgt gtcctggctt ggccctccca cgctcctaac
     5641 tgcgctcttg cgccacgccc caaccggtcc cggaactacg tttatcgcat ttacacctcc
     5701 aaaggccatt ttgtctatat tttgaccaac tatgtactcg atcctctgaa gcacatcaat
     5761 aaaggagcgc gaacgtgcag tcgcgatctt gccagccact gcgccaactt tccggtccca
     5821 aaatctcgca acaagttttg atatagtgtt tttcttaaaa atggtgtcag cgtccataat
     5881 cacgcacacg tcgaaagtgg acacctccat tccgatgcgt atggcggttg ccttgccatt
     5941 gtttggcgtg cgaatcaatt ttattcccga atagacaact gcgagatgtg ctacgatacg
     6001 tgccgtactg tctgttgagc cgtcgtcaac gacgataatt tccttttttt gataatcatt
     6061 gaagacgaca ctttttatcg ttgattcaat attctcttcc tcgttataag ctggtatcag
     6121 aacgctaacg ccgtcattgt agcggtgcgt tgatattctt cgtgcatcct ttatttttga
     6181 aaacccgaga agcaggaaca tacatactat acgaagtgag acaagaatta aaaccgcgaa
     6241 ataaaatata tacaaacacg taaggataaa tgccgcacta agcgtgtata cagcttcagg
     6301 ccatacgctt ttagtactaa tcctttgact aatgtaatgc gtctccaact cccccgatgt
     6361 ataacgcttc gacgcttctc taat
//
LOCUS       DATDSU010000127         3593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_780659, whole genome shotgun sequence.
ACCESSION   DATDSU010000127 DATDSU010000000
VERSION     DATDSU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780659"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..699
                     /locus_tag="VI981_04430"
     CDS             184..699
                     /locus_tag="VI981_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479570.1"
                     /translation="MRTRNVFTAATLSLLLVAFSASVALAQSDKGKEVSEEQRSKVAE
                     TVQALVDLAGKDNNIGGEILAVAQEQEVSNEHVTKAKEAIEVRGGLKTFLIGTDYKNI
                     GALRSEVVTTQNSIDRLTKAKERATDDVVKVVLDAQIKALEEVNTNTLNFIQTNESKF
                     SLFGWFVRLFQ"
     gene            1010..2125
                     /gene="cfa"
                     /locus_tag="VI981_04435"
     CDS             1010..2125
                     /gene="cfa"
                     /locus_tag="VI981_04435"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015266822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEY4479571.1"
                     /translation="MFNEKEVVQNILKKAGVEINGDHPWDIQVHDERLYRRVLVYGSF
                     GLGEAYMEKWWDCERVDLFIEKILRSGQYANAKTLPLVLLTVKATLLNLQSRIRAKEV
                     IERHYDLGNDLYLSFLDPYNQYTCGYFLETDNLNEAQEKKLDLICRKLQLKQGDNLLD
                     IGCGWGGFAKFAAEKYKCRVVGITISDEQLKYARQYVAGLPVEIRKMDYRDLSGEKFD
                     KITMIGMIEHVGYKNYRRVMEIVNESLGDDGLFLLHTIGQNVTSTSGNPWSDKYIFPN
                     GMLPSAKQLAKASEGIFVMEDLHNFGPYYHDTLRAWDANFQKNWTNIENRYSETFYRM
                     FRYYFNSFAGAFKSRRIQLWQIVFSKGTSDRVYESVR"
     gene            complement(2580..2968)
                     /gene="ssrA"
                     /locus_tag="VI981_04440"
     tmRNA           complement(2580..2968)
                     /gene="ssrA"
                     /locus_tag="VI981_04440"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3020..3172)
                     /locus_tag="VI981_04445"
     CDS             complement(3020..3172)
                     /locus_tag="VI981_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479572.1"
                     /translation="MSAKKVAKQIAEQFANTATKVLATKAARSQREKALRRLTDKLVR
                     KKKTYH"
     gene            complement(3209..>3593)
                     /gene="smpB"
                     /locus_tag="VI981_04450"
     CDS             complement(3209..>3593)
                     /gene="smpB"
                     /locus_tag="VI981_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY4479573.1"
                     /translation="AGLELLGFEVKSLRAGHGQLEGAHVVVRGGEAYLVGATIPPYQT
                     GNTPKEYDPARTRRLLLTKKELGELAQYENKKGLTIVPLSVYNKNSRLKLSAAVARGK
                     KKYDKREVLKKRDTEREIRRTLKTE"
BASE COUNT          993 a          756 c          864 g          980 t
ORIGIN      
        1 actccaaagg ccagcttacc gataccgagg taagctcgca atgcaaaaca ggcgtttata
       61 ctcttgaaaa tcgcattgac cgagaggtac agttacaggt aaatggatta agcgactatt
      121 tcagttctcc aaccggaggc aagggaagaa ataagtaata ttattagtat aatcaatgtg
      181 tatatgagaa caagaaatgt ttttactgcg gccactctct cgcttctgct cgttgctttc
      241 tctgcttccg ttgcgttggc gcagagtgac aagggcaagg aggtgagcga ggagcagaga
      301 agcaaggttg ccgaaaccgt gcaagcgctc gttgatcttg cgggtaaaga caataatatc
      361 ggtggggaga ttctcgccgt tgcgcaagag caagaagtat caaacgagca cgttacaaaa
      421 gcaaaggaag cgattgaagt acgaggcgga ctcaagacat ttctcatcgg tacggattat
      481 aaaaatatcg gcgcactcag aagcgaagtg gttactactc aaaacagcat tgaccgtctg
      541 acgaaagcga aggagcgagc gacagacgac gtggtgaaag ttgtcctgga tgcgcaaatc
      601 aaggcccttg aggaagtgaa cacaaacacc ttgaacttta tccagacgaa cgaaagcaag
      661 ttcagtctct ttgggtggtt tgtgagactt ttccagtaat agtttccggg aatgaaaaaa
      721 cagactgatg gtcaagccgt cagtctgttt tttgacactg tgcgcaaaca acgggaagga
      781 agattttatt cttctacatt gttctatatc agtggagtct caatgaatga cgttatttat
      841 cctgagcttg tcgaaggaga acctctttgg aggaaaacga aaatatgacg tatataaaag
      901 cttttgtaga atttgcaaaa acatgtggag aaagggttgt ctagaccagt tcactgaccg
      961 aagttagaac cccctaaaat tgaaggcgta tccgaggtaa tatttgctta tgtttaatga
     1021 gaaagaagtg gttcaaaaca ttttgaaaaa ggcaggtgta gaaataaatg gcgatcaccc
     1081 gtgggatatc caagtgcacg acgaaagatt gtaccgacgt gtgttggttt atggatcatt
     1141 tggattggga gaagcctata tggaaaaatg gtgggactgc gagcgagtcg atttatttat
     1201 cgaaaaaata ttacgatcag ggcaatatgc taacgcgaaa acacttccgc ttgttttatt
     1261 aaccgtaaag gcgactctct tgaatttaca gtcgaggatc cgtgctaaag aagtcattga
     1321 acggcattac gacctcggca atgatttgta cctatcattt ttagacccat acaatcaata
     1381 tacctgcggg tattttctcg aaacagataa tctgaacgag gcacaagaaa agaaactcga
     1441 cctcatttgc agaaagcttc aattaaaaca gggcgacaat ctgcttgaca tcggttgtgg
     1501 ttggggtggt tttgcaaaat ttgctgctga aaagtacaag tgtcgagttg ttggtattac
     1561 catttcggac gagcaactga agtatgcgcg ccaatacgtt gctgggttgc cagtcgagat
     1621 ccgaaaaatg gattataggg atttgtctgg agaaaagttc gacaaaatta cgatgattgg
     1681 gatgatcgag catgtcggtt ataaaaacta caggagagtg atggagatag taaatgaatc
     1741 tttaggagac gatggacttt tccttttgca caccataggt caaaacgtga cttcgaccag
     1801 tggaaatcca tggtcagata agtacatttt tccgaatggg atgcttccat ctgcaaagca
     1861 actggccaag gcgagcgagg gaatttttgt aatggaagac ttgcacaact tcgggcctta
     1921 ttaccacgac actttgcgag cgtgggatgc caattttcaa aagaattgga cgaacataga
     1981 aaaccgttat tcagaaactt tttatcgaat gtttagatac tacttcaaca gttttgctgg
     2041 cgctttcaag tcgagacgga ttcagctatg gcagattgtt tttagcaaag gcacaagcga
     2101 tagagtttat gagagcgtgc ggtagtgcca cctcttttct ttatccgcgt tactccgagg
     2161 cccatccaag gcccgttttg ttttttgata ggggttctta taggctagtg tttcggaaac
     2221 ttggcgcaat caaaatagaa cctccaatcc gtaatgacgc gcattacgcg atatggaaga
     2281 acgtgaataa tatgtatgtg gagccactcg cgatttattt tgtggcgccg acatatgaat
     2341 gtcggcatcc tacaaaatta tagtcgccga ttacggctcg ttaattttgt ggagatgggc
     2401 ggagtcgcct cacgagttac taattcgctt cgctcataag tactctcgag cccgtctcgg
     2461 ctccttcgca agctcaggat gcctccgact gttcgactac cgccgcatgc aaagcaattt
     2521 gcatgctccc atctccatag acaaaaaacg cccgcctttc ggcgagctgg tttttgtttc
     2581 tatggagatg ggcggagtcg aaccgccgtg caagaaaggt tttcagatga atctacaagt
     2641 ttagtgtgct ttgtgtttta aagacactgc gttaaaaaca aaccaaatcg cagtccctcg
     2701 agctcttgat tttcgtacat ggcgccgagc atgcgccata tcttatcctg acgatataac
     2761 acctctggca aatagtcagg cagtctctgc cgagatggta gtctaaaaaa ttaggctaca
     2821 gcgtaagcag gctgtgcgaa cagtgccgca aaaccagctt tcactttcga taatgcgttt
     2881 gcatgtatcg gtgtggagca gtttaagaag ttactccagc ttcacctgca caactgaaaa
     2941 atattccttg tcgatgcctg gcatccccaa aatttgtatt cgcatcgatt gcgaaaagtg
     3001 aggggtgacc gcagtcaccc tagtggtagg ttttcttttt tcttacgagt ttgtctgtaa
     3061 gacgtctcag agctttttct cgctgagacc tcgcagcctt ggtagcgagt accttggttg
     3121 cagtgtttgc aaactgctcg gcaatttgct tcgcaacttt cttagccgac atttgtttct
     3181 cccaaataaa cccacttttc gcaatcgatt attctgtttt caatgttcgc cgaatttctc
     3241 gctcggtatc gcgcttcttg agtacctcgc gcttatcgta ttttttcttg ccgcgagcaa
     3301 ccgccgccga gagttttaac cgtgaatttt tattatatac tgaaaggggc actattgtca
     3361 agcctttctt attttcatac tgggcgagct caccgagttc ttttttggta agtaagagcc
     3421 gccgtgttcg cgccgggtca tactctttcg gggtattgcc ggtttggtaa ggaggtatag
     3481 ttgctcccac caagtacgct tcgccaccac gtacgacgac atgtgcgccc tcaagttgtc
     3541 catgacccgc tcggagcgat ttcacttcga aaccaagcaa ctccaaaccc gcc
//
LOCUS       DATDSU010000128         5564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_297792, whole genome shotgun sequence.
ACCESSION   DATDSU010000128 DATDSU010000000
VERSION     DATDSU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297792"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="VI981_04455"
     CDS             <1..1729
                     /locus_tag="VI981_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HEY4479574.1"
                     /translation="TVTGPGINCGADCREYIAPGSSLSLDQTPQSGWSFYGWSGACSG
                     TGSCNLLMDSAKIINATFATSAPSPLSVSCSASPTSINRGDASSWGATITGGLGSYTY
                     NWSGTDGLLGTTPSVAQVYNTVGTKSASVTVTSGAEQVTRACSNTITVGDQAALSVSC
                     SGSPTSINTNDTANWTAAVSGGLGSYSYSWAGTDSLGGSSASVSKVYTAQGTKSADVT
                     VASGSEQITRACGNSISVTVPTAALQASCSVNPTSASTNQNVTWTSSVSGGTGAYTYS
                     WSGSDALSGNTSSVTRAYTSGGTKSAAVAVTSGTQQVNPTCSNSVSVTTGPPIITAQE
                     IALHNAQSNCWWYIGTNVYNMTAYFGRHPGGNSRMLPYCGTNAEPGYNSRNHSNTADT
                     QLASFQIGILGTVAQQNTASSLSQNMAQVFSSPAKPVRISLSAVAMSAFHANLVWKPL
                     VEANIKEYSIIRNGELISTVDATGFVDTALSPAAGYTYKVVGLDGKGKALYESAKVTI
                     KTKARPICGLDVLGVIPCLVEQNYPGAVLKTQNIRQNGAQLLKILWRGQCRAIGTVPD
                     GTLVEDIKC"
     gene            1723..2613
                     /gene="hemC"
                     /locus_tag="VI981_04460"
     CDS             1723..2613
                     /gene="hemC"
                     /locus_tag="VI981_04460"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY4479575.1"
                     /translation="MLEIRIGTRGSELALCQATLAREAILKNYPEANVTLKVIKTTGD
                     VKLGPIPETVVGKSWFTKEIEDELLNETIDLAVHSLKDMALMPPPELYIGAMLPREDA
                     RDVLVTRNKEKLKELKSAASIGTDSARRGIQICALQPDLKVRSVRGNIGTRLLKLDSM
                     EYDALVLAAAGLVRLGLIDRVAQFFSFDEILPSPGQGILAIELKRSRKELVEITRNLN
                     HRETELVGRTERAFSETYGGGCKKPVAAYAEIVGNSISLRAMVANDAGVITKRTAVSD
                     LYEGQKMAQELAVQLLDATK"
     gene            2600..3337
                     /locus_tag="VI981_04465"
     CDS             2600..3337
                     /locus_tag="VI981_04465"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY4479576.1"
                     /translation="MRRNKDIVVLTGSDEFIAKLKPLLSLKKKSILKLTTIVSAPLNI
                     KKSTAKGILGLEKTDWIVFSSKNAVRFFIKNVSHSTKSKIAAVGGETAKLTIRLFGRV
                     DFVPLAFNMRELSHTVPGVKGRRILLFGAKNGRNAVLQLRKRGAIALNIELYSVKLEK
                     TSNKELRALSSRIKAVLFASPSSVRSFVFQSGGYKYAHDILHNAAAFALGPSTGKALK
                     KIGAKRIITTHQSTARGMATAFNKWNG"
     gene            3325..3996
                     /locus_tag="VI981_04470"
     CDS             3325..3996
                     /locus_tag="VI981_04470"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY4479577.1"
                     /translation="MERINPLYHHYLLLETSRSALSLGEKERADLKKEFIRALKTSPL
                     AIFSYSVKGFRSNASLLLWLSGDSLSEIQVCVNELTGGKFRSLVKIREVYFGKTGPTQ
                     YKKGGLPSREIKVSAPRRKYLIVYPFTKTTEWYLLPFNKRKIMMTEHIKIGMRYSAVD
                     QLLLYCFGMNSHEFVVSYETDKIDEFQDLVMELRGARGRRYTANDTPVTLGIYLSHEE
                     VVRYI"
     gene            3998..5017
                     /gene="hemE"
                     /locus_tag="VI981_04475"
     CDS             3998..5017
                     /gene="hemE"
                     /locus_tag="VI981_04475"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY4479578.1"
                     /translation="MKHNSLFLDAAYCKKTARAPIWFMRQAGRYMEEYRKLRQKFGIL
                     EIATTPELATEVCLQPIRAFHFDAAIIFADIILPLIPLGVKVTIADGVGPVVDEPITR
                     LEQVNKFDTVEWQRDFAFLEKTIKMVRKELGETPLIGFSGAPFTLAGYLIEGGPSRAF
                     LKTKSLMYREPKVWDTLMKKLTSAVITYLQGQIESGVQAVQLFDSWVGVLSPRDYETY
                     VLPYSQEIFSALGNKRVPRIHFGTNTFGMLQEFSRVDCEVIGVDWRSPLGASRNSIGK
                     KAIQGNLDPALLLAPFEIIKKRVDGIFNEIGSKPGFIFNLGHGILPETPRDTVQRLVE
                     YVQSK"
     gene            5205..>5564
                     /locus_tag="VI981_04480"
     CDS             5205..>5564
                     /locus_tag="VI981_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY4479579.1"
                     /translation="MTVKKNTKGVLLCAYGSPNTKVEVKPYLEDIKGGPVSPHVVSIF
                     EKRYAEVGGKTPLYEITKSQAELLEKELIKMNNPARVFVGMKHWHPYIADVVREAAKE
                     GIRDLKVLALAPHYSKIS"
BASE COUNT         1580 a         1234 c         1318 g         1432 t
ORIGIN      
        1 gacggttaca gggcctggga taaattgtgg agccgactgt cgcgaatata tagctcctgg
       61 aagttcgctc tcgctcgatc aaacaccgca aagcgggtgg tcattctatg gctggtcagg
      121 tgcttgtagt ggcacaggat cctgcaattt actcatggat tcggccaaaa taatcaacgc
      181 tacatttgcc acctctgctc cttcacccct tagcgtaagc tgcagtgcgt cgccaacttc
      241 gattaaccgc ggagacgcct catcctgggg ggcaaccatt acgggcggtc tcggttccta
      301 tacgtataat tggtcgggta ctgatggtct cttgggtaca actccttcag tggcccaagt
      361 ctataataca gtcggcacta aaagcgcaag cgtgacggtg acctcgggtg ctgaacaggt
      421 cacgagggcg tgcagtaaca cgatcacggt aggtgatcag gccgctcttt cggtcagttg
      481 ttctggctct ccaacttcca taaacacaaa tgacacggca aactggacgg cagctgtttc
      541 gggcggtctc ggttcctact cctacagctg ggcgggaaca gattctcttg gtggttcatc
      601 ggcttcagtc tctaaagttt atacagcgca aggcacaaaa agcgcagatg ttacggtggc
      661 atcgggatcg gaacaaatca ctcgggcgtg tggtaactca atttccgtaa ctgtgcccac
      721 agccgctctt caagcatcat gcagtgtaaa tccaacttcg gcctcgacca atcaaaatgt
      781 tacttggacg tctagtgttt ccggtggtac cggagcatac acatattcct ggagcggcag
      841 cgatgcgctc tccggaaata cttcgagtgt cacgagagca tatacatcgg gaggcacgaa
      901 gtctgcggca gtcgctgtca cttcaggtac acaacaagtg aatccgacat gctcaaatag
      961 cgtatccgta actacgggtc ctcccatcat taccgctcaa gaaatagccc tacacaatgc
     1021 tcaaagtaat tgctggtggt atatcggaac taatgtatat aacatgacgg cctatttcgg
     1081 tcgccaccca ggcggaaaca gccgaatgtt accgtactgc ggtaccaacg ctgaaccggg
     1141 ctacaattcg agaaatcatt caaataccgc agacacacaa ctcgcatcat ttcaaatcgg
     1201 aatactggga actgtggcgc aacaaaatac cgcctcaagt ctgtcgcaaa acatggccca
     1261 agttttttcg tctccggcaa agcctgtgcg tatttctctc tcagctgttg ctatgtccgc
     1321 ttttcacgca aatctagtct ggaaacctct tgttgaggct aacataaaag aatattcaat
     1381 tattcgaaat ggggaactca tcagcactgt tgatgcaact ggtttcgtcg atacggcctt
     1441 atccccggcc gcaggctata cgtataaagt cgttggtctc gacggaaaag ggaaagcact
     1501 gtatgaatct gcaaaagtta ctataaaaac taaggctcga ccgatctgcg gccttgatgt
     1561 actcggtgtt attccgtgtt tggttgaaca aaactacccg ggtgccgtac ttaaaacaca
     1621 aaatataaga caaaacggcg ctcaactatt gaaaatactt tggcggggac aatgcagagc
     1681 gatcggcacg gttcctgacg gtactcttgt cgaagatata aaatgctaga aattcgaatc
     1741 ggtactcggg gaagtgagct cgcactctgc caggcgactc tcgctcggga agcaattcta
     1801 aaaaattatc cggaggcgaa tgttactcta aaagttataa agacaactgg ggatgtgaaa
     1861 ttaggtccca ttcctgaaac ggtagttgga aaaagctggt ttacgaagga aattgaagat
     1921 gaacttctca acgagacgat agatctcgct gtacatagtc taaaagacat ggcgctcatg
     1981 ccgccacccg agctttatat cggtgcgatg cttccgcgtg aagatgcgcg cgacgtactt
     2041 gtcaccagaa acaaagaaaa attaaaagaa ttaaaaagtg cagcctccat aggtacggat
     2101 agcgcaagga gaggtataca aatctgtgcg ttacagcctg atcttaaagt cagaagtgtt
     2161 cgaggaaaca tcggaacgag gctattaaag ttagactcca tggaatatga cgcattggtt
     2221 ctcgcggcag cgggtctcgt gagacttggt ctcatcgatc gagtggcgca atttttttca
     2281 ttcgacgaga tactgccttc tccggggcag ggtatcctgg ctatagagct caagcggtca
     2341 cgaaaagaac ttgtagaaat tactcgaaat ttgaaccacc gagaaacgga acttgtggga
     2401 cgtactgaac gcgcgttttc tgaaacgtat ggcggtggct gtaaaaaacc cgtcgccgcc
     2461 tatgcggaaa tagtgggaaa ctcgatttca ctccgagcca tggtcgcaaa tgacgcaggc
     2521 gtcattacaa aacggacggc tgttagcgat ttatatgaag ggcaaaaaat ggcacaagag
     2581 cttgctgtcc aacttcttga tgcgacgaaa taaagacata gttgtgctta cgggttcaga
     2641 cgagtttatt gcaaaactca agcctttatt atcgcttaaa aaaaaatcta ttttaaaatt
     2701 gaccacgatt gtgtcagcgc ctttaaatat aaaaaagagc actgcaaaag gaattctcgg
     2761 tcttgaaaaa acagactgga ttgttttttc aagtaaaaat gcagttcgat tttttataaa
     2821 aaatgtcagc cattccacaa aatcaaaaat tgcggcggtt ggcggtgaaa ccgcaaaatt
     2881 gacgatacgg ttattcggcc gagtagattt cgtaccgctt gcattcaata tgagggagct
     2941 atctcacacc gtacctggcg ttaaaggacg ccgcatcttg cttttcggcg caaaaaacgg
     3001 aagaaacgct gtactgcaac tccgaaaaag aggcgccatt gcgttgaata ttgagctcta
     3061 ttccgtaaag ctcgaaaaaa cttccaataa ggagcttcgt gcgttgagtt cacgcatcaa
     3121 agcggtcctt ttcgcgagcc cttcatcggt tcgatctttc gtatttcaat caggggggta
     3181 caaatatgct catgatatac tccataacgc cgctgctttt gcgctcggac caagcacagg
     3241 caaagcactt aaaaaaatcg gcgcgaagcg gattatcaca acgcaccaat cgactgcgcg
     3301 aggtatggcg accgcattta ataaatggaa cggataaatc cactatatca tcactatctt
     3361 ttactcgaga cgtctcgaag cgctttatcg cttggtgaga aagaacgtgc cgatttgaaa
     3421 aaggaattta ttcgtgcact caaaacatct ccgcttgcta tcttttctta ttcggttaag
     3481 ggttttagga gcaacgcttc gcttttgctc tggctttcag gtgattcgct ctccgaaata
     3541 caagtttgcg taaatgaact tacaggcggg aaatttcgct cgcttgtgaa aatccgagaa
     3601 gtttattttg gcaaaacagg accgacacag tacaaaaagg ggggattgcc gagtcgtgaa
     3661 atcaaagttt ccgctccgcg ccgaaaatat ttaatcgtgt atccgttcac taaaacaacc
     3721 gagtggtatc tactgccgtt caataagcgg aaaataatga tgactgaaca tattaaaatc
     3781 ggtatgcgtt actccgccgt agatcagttg cttctttact gtttcggaat gaatagtcat
     3841 gagttcgtcg tctcatacga aacagataag atagacgagt ttcaagattt agtcatggaa
     3901 ttacggggag cacggggacg ccgttatacg gcgaacgata cgccggtgac actcggcatt
     3961 tatctgtctc acgaagaagt tgttcgctac atataacatg aaacataatt cgcttttttt
     4021 agatgctgcc tattgtaaaa aaacggcacg agcgcccatt tggtttatgc gccaggctgg
     4081 aaggtatatg gaagaatatc gaaaattacg ccagaaattt gggatactcg aaatagcgac
     4141 gactccagaa cttgcgactg aagtctgtct tcagcctatt cgagcgttcc actttgatgc
     4201 tgccattatt tttgccgata ttatcctgcc gctcattccg ctcggcgtga aagttactat
     4261 tgctgacgga gtaggccctg ttgtcgatga accgataact cggttagaac aagtcaataa
     4321 attcgataca gttgaatggc aaagagattt tgcatttctt gaaaagacaa taaaaatggt
     4381 tcgaaaagag ctgggagaaa cgccgctcat tggattttct ggcgccccgt ttactcttgc
     4441 cggctacctt atagaaggag ggccttcgcg tgcgttttta aaaacgaaaa gtttgatgta
     4501 tcgcgaaccg aaagtttggg acacgttgat gaagaaactg acgagtgctg ttataacata
     4561 tcttcagggt caaattgagt caggtgtgca ggccgttcag ctctttgaca gctgggtggg
     4621 tgtgctttca ccgagagatt acgaaacata tgttttgccg tattcacaag aaattttctc
     4681 cgcgctcgga aataaaagag tgccgcggat tcattttggc acaaacacat tcggtatgct
     4741 tcaagaattt tctcgcgttg attgcgaagt tattggagta gattggcgtt cgcccctcgg
     4801 agcatcacgc aatagtatcg gtaaaaaagc cattcaggga aatcttgatc ctgcgctttt
     4861 actggcgccg tttgaaatca ttaaaaaaag agtcgacggt attttcaacg aaatcggatc
     4921 aaaaccaggc tttattttta atctaggtca cggcattttg ccggaaacac cccgagatac
     4981 cgtgcagagg ctcgttgaat atgtgcaatc aaagtgatcc cgttagagat cttattgtta
     5041 tatattattt actatgttga ggttatacga taataagcaa ataaaagttg agcgatttat
     5101 gacggaaacg cctcttctgt tcactttatc tttaggtgtc acattatcaa tcaagctaaa
     5161 cgcaaagctt ctcatcagga agagcgtttc ctatctctaa cgggatgacg gtcaagaaaa
     5221 atactaaggg agtcttgctt tgcgcgtatg gtagccccaa tacaaaagtg gaagttaaac
     5281 cgtatctcga ggatataaaa ggcggtcctg tgtctcctca cgttgtttct atctttgaaa
     5341 aacgatacgc agaagtgggc gggaaaacgc cactttatga aattacgaag tcacaagccg
     5401 aacttctcga aaaagaactt atcaagatga ataaccccgc gcgggtgttt gtcggtatga
     5461 agcattggca tccgtacatt gccgatgttg taagagaagc ggctaaagag ggaatacgtg
     5521 acctcaaggt tctcgctctc gcgccgcact attcgaaaat tagt
//
LOCUS       DATDSU010000129        12131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_640997, whole genome shotgun sequence.
ACCESSION   DATDSU010000129 DATDSU010000000
VERSION     DATDSU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12131
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640997"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VI981_04485"
     CDS             <1..382
                     /locus_tag="VI981_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEY4479580.1"
                     /translation="IREAVIDIRQKKLPDPTKIGTAGSFFKNPIVTKLQFQKLKMRFP
                     ALPSFAAAGGKVKLPLAWILDKICGLGDYREGNVGLYLHQPLNVVQYGDATAIDVLRF
                     AERVAQIIEEKTSIKPQREVRMVE"
     gene            387..1157
                     /locus_tag="VI981_04490"
     CDS             387..1157
                     /locus_tag="VI981_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479581.1"
                     /translation="MNKRIQIILLCFLAPVLLAAVMFSQINNIVEIETGGETAKSSQT
                     QEKKGCDIKENRYGEGFHGVDIRGGVVCYFNITKNKDPYIFVVTANERNQPQTIDVYY
                     ATKLVQTLNLGPEKQSPESPVSTDFIDVDDINFDGKNDLRIVQSTGVTGNVYYLFYVF
                     NPKLSSFEYSEEFSQLPNYARDPEKKIITTHINTGAAGAAFVEETYEVKNDTLVLVRY
                     VEQRVHPDYGDSFLKTTKVLRGGEMIIEKEERVLAPFN"
     gene            1256..3778
                     /gene="secA"
                     /locus_tag="VI981_04495"
     CDS             1256..3778
                     /gene="secA"
                     /locus_tag="VI981_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY4479582.1"
                     /translation="MSFLKALLGDENQKTLKKYHPLVDRVNVLEAEFEKFDETALRAK
                     TAEFRERLNKEETLDALLPEAFAAVREASKRTLGQRHFDVQILGGIILHKRGIAEMRT
                     GEGKTLVATLPAYLNALGGKGVHIVTVNDYLSRRDAVWMGQIYAYLGISVGVINHDTS
                     FLYDPTHKELDKERDEIASFKVVYDFLRPSTRKEAYEADITYGTNNEYGFDYLRDNIE
                     YDISDLRQREFNYAIVDEIDSILIDEARTPLIISAPTTESENLYAVFAKISERLNKDE
                     DYTVDEKFKSIVLTDKGIEKAEKVLGVDNIYTEKGIKYVHHLETAVRAKALFHKDKEY
                     VVKDDEVIIVDEFTGRLQPGRRWSEGLHQAIEAKEGARVQRESRTYASITFQNYFRLY
                     KKLAGMTGTALTSKEEFLKVYGLDVLEIPTNKPMARADHNDLIFQTEKGKFKAIAKKV
                     KELNKKGQPVLIGTVSIEKNELLSSYLKSEGVEHVLLNAKPQHAEKEGEIIAQAGRKG
                     AVTISTNMAGRGVDIKLGGNPATTEQYETIKNNGGLFVLGTERHEARRIDNQLRGRSG
                     RQGDPGETQFFVSLEDHLMRVFASDTIKKLMGRLGIAEDVPIENRIITRSLETAQTKI
                     EGFNFDSRKHVLEYDDVLNIQRKTVYSRRRKILTGALDDIEEELMLIVGDDEKLFKVI
                     EEKEKLLGEEEFWKAVKRLFLQAIDMFWVDHLEMMDYLRSSVRLRAYGQRDPLVEYKR
                     EGLGLFKEMQEATNAQILEMLPNIGAGAFKNEEQRAREVQKQMKLITDEANQGRTLNQ
                     QKGPTLATPSGEKIGRNDLCPCGSGKKYKKCGLINASFHKKS"
     gene            3832..4179
                     /locus_tag="VI981_04500"
     CDS             3832..4179
                     /locus_tag="VI981_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479583.1"
                     /translation="MAYKPQSILIEEVSDEQLLGGYDARVEELYLEKENFERNGITRR
                     IGHYEFDETINSISKEIERTKRLRNALIERMKLKTKKQIWYKDPDILVKIILALIGAL
                     ATVIAAWIVSSRK"
     gene            4232..4798
                     /locus_tag="VI981_04505"
     CDS             4232..4798
                     /locus_tag="VI981_04505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY4479584.1"
                     /translation="MLNLLKEIVIYDTEYTTWEGAQKRNWSGPGEYREVVNIGAIKIE
                     TENFRVLDSLDLFIKPFKNPILSKYFISLTSITQEEVDTKGISLKVAMGEFVKWCGRL
                     QLYSYGPDHERLIENCALIDIPFLLKKENCHDVRDIFRMHGIPAENYMSSTIVRAFGQ
                     EPTRQAHTGVDDSRAIADALRLLKLREH"
     gene            4870..5451
                     /locus_tag="VI981_04510"
     CDS             4870..5451
                     /locus_tag="VI981_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY4479585.1"
                     /translation="MLRKCRRCGAGFEPHQWNHVFCGSKIQQTGCSWLNLTSDRSKRR
                     WQNEKYRAYQKEYGKIWKRIQRKLNADYAVRQRLLKREAALTPEGKKVAQAWRRRNLV
                     KVLFWNKKRARIKRGVVGSHTFVDWVSIKSKYNYCCAICKLSESDLRKKWKNTHFTEL
                     TMDHILPISKGGTDYITNIQPLCISCNAKKRDA"
     gene            5444..5905
                     /locus_tag="VI981_04515"
     CDS             5444..5905
                     /locus_tag="VI981_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEY4479586.1"
                     /translation="MPKKKQIVVAVSGGMDPLHIGHVRMLVEAKKLGSKLIVILNNDN
                     WLMKKKGFVFMKDTERKEVIENINSVDEVYLTEHAPDTTDMSVCVALGKIRPHVFANG
                     GDRKPDGDPVPEVEICRELGIKMVYNIGSGGKVQSSSWLVKKYLETQNKKK"
     gene            5969..6403
                     /locus_tag="VI981_04520"
     CDS             5969..6403
                     /locus_tag="VI981_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4479587.1"
                     /translation="MTHTYIAGGVVLNKKGEVLIVNQLGLSWSLPKGHIEEGEDELAA
                     AKREIFEESGISQLSVIKRLGSYDRARIALDVTKEDKREIRTITLFLFTTPQMTLEPR
                     DAENPEARWVPRQEVTDYLTHRKDRYFFKNIIPEIDELFPPK"
     gene            complement(7052..8746)
                     /locus_tag="VI981_04525"
     CDS             complement(7052..8746)
                     /locus_tag="VI981_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HEY4479588.1"
                     /translation="MAYRKNTLFRFLTAGLTLLVFTYVSTISPFIGDGSFLNAASSID
                     VWWPVENAKIDGAQPFKALLRDTDVSNYEMFWQVDNGKWNKMENNERDWPHKEAGVDV
                     SNWTWHESRPYEIKFIAQQSGTIVEERTIHIVVENKTNASKTSDSESNTDGVSAPDTS
                     QNEVVSTSESSRTADTAASPSQQLRERILSHVINQINGGDSPTPSPIPAPASSPSPSP
                     TPTPAPAPAPRSTPVPDSPPNPKENSTSNPSPAPTPVPAPTATPDPKPVPPPPTSAPS
                     NPLDGVRFYVNKDSKATEFVNEWKNSRPADANLMSKIASGAESVWLGDWSDIPQDISS
                     VISKAQSAGEYPVFIAYNIPGRDCGGYSAGGVGSSGAYRDWINSIVNTIGNNKAAVVI
                     EPDALSNITCLSPSLQSDRLDLIGYAVRTLKAKPNITVYVDAGNPGWLPAETIAPRLQ
                     SAHVAEADGFAINISNFYTTADNIRYGEAISRLIGNKHFIIDTSRNGAGSNGEWCNPS
                     GRALGQKPTSQTNNALIDALLWLKKPGESDGTCHGGPTAGVWWTEYALGLASRASW"
     gene            9258..11582
                     /locus_tag="VI981_04530"
     CDS             9258..11582
                     /locus_tag="VI981_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEY4479589.1"
                     /translation="MAKKTVESKKVESAIELVKKIQKRDGSVVAFDFAKIVRAIHKAM
                     IQTGEGSEKEAELVANKVYAELVRIAKKFKTFVPTVEGIQDTVEKELILSEYVKTAKS
                     YILYREKRAKLREKGLQVPVKVKKLAEESKKYFRNSLGEFVYYRTYSKWIVEENRRET
                     WIETVDRYIDFMKENLGGKLKDAEYKEIQEAILRHEAMPSMRLLQFSGKAARSTHVCA
                     YNCSYIAPESFQDLAEIMYISMCGTGAGWSVESQNVQKFPQILPQTGEKLPTHVIPDS
                     KEGWADSLTLGMKTWALGKDVEFDYSELRLAGTRLKTMGGKSSGPDPLRRLLTFTREA
                     MLIRQGKRLRNLDVHDIICMIGDCVVAGGVRRSAMISLSDMDDDLIRDAKKGAFYVTH
                     PHRSLANNSAVYLKKPTNEEFLKEWMALIESRSGERGIFNRGSLAKTLPARRIKVLKE
                     HKGYFDSTGEMIIGSIGTNPCGEIILQSKQFCNLSEVVARREDTEATLLRKVRVATIL
                     GTYQSSLTNFPYLSKEWREHCERERLLGVSITGQWDSPVVRQPDVLKKLKNESIKINE
                     QYAKRFGISASTCITCVKPSGTLSQTVDCSSGMHPRHAPYYIRRIRISTTDALFKMLK
                     DQGVPFHPEVGQPAETASTFVLEFPVKAPEGSIYKNDITAIDQLEHWKVVKVNYTEHN
                     PSVTVSVGDNEWIDVANWIYKNWDIVGGLSFLPRDNHVYQLAPYEPIDEKKYKELSKR
                     LAHIDYSKIITYERQDETEVKKELACVAGVCEIV"
BASE COUNT         3583 a         2425 c         2913 g         3210 t
ORIGIN      
        1 tattcgggaa gcggtgatcg atatacgaca aaaaaaactt ccggatccga caaaaattgg
       61 cacggcgggt tcttttttta aaaatccgat tgtgacaaaa ctgcaattcc aaaaactaaa
      121 gatgcgtttt cctgcattgc cttcgtttgc ggcagcgggc ggcaaagtga aattaccgct
      181 agcctggatt ttagataaga tctgcgggct tggcgactac cgagagggga acgtcggcct
      241 gtatctgcac caacctctca atgttgttca gtacggtgat gctaccgcaa tcgatgtact
      301 ccggtttgcc gagcgcgtgg ctcaaatcat agaagagaaa acctcgatca agcctcaacg
      361 ggaggtgcga atggtagaat agaaatatga acaaaagaat acaaattata ttgctttgtt
      421 ttcttgctcc cgttctcctt gccgcagtga tgttttctca aataaataat atagtagaga
      481 tagagacagg gggagagacg gcaaaaagct cgcaaacaca agaaaaaaaa ggatgcgaca
      541 tcaaagagaa tcgatacgga gaaggatttc atggcgttga tattcgtgga ggtgttgttt
      601 gttatttcaa tattaccaaa aacaaagacc catatatatt tgtcgtgacc gctaatgaac
      661 gaaaccaacc ccagacaata gacgtatact atgccacaaa actggtgcag acgcttaact
      721 taggaccaga aaaacagagt cctgaatcgc cagtttctac tgattttata gatgttgatg
      781 atataaattt tgacggcaaa aacgatttac ggatcgtgca gtctaccggc gttaccggca
      841 atgtttacta tttgttctat gtatttaatc ctaagcttag ttcgtttgaa tacagcgagg
      901 agtttagtca gcttccaaat tatgcacgcg atcccgaaaa aaaaataatc accacgcata
      961 taaataccgg tgctgcaggc gcggcctttg ttgaggaaac atacgaagta aaaaatgaca
     1021 cacttgtact cgtgcgctat gtagagcagc gcgttcaccc ggattatggc gattcttttt
     1081 tgaagacaac aaaagtgctg cgcggtgggg aaatgattat cgaaaaagaa gagcgagttc
     1141 tagcgccatt taattaatgt tgaccaaggc ctggccttag catactaagg ccaggccttg
     1201 gtggatcctg tgcgcggatt ttacggagag gcgcttctcg tatagtatta cccctatgtc
     1261 ttttttaaag gccctgttgg gcgatgaaaa ccagaaaacc cttaaaaaat atcatccgct
     1321 tgttgatcgt gtaaatgtgc ttgaagcaga attcgaaaaa tttgatgaaa ctgcgttgcg
     1381 ggctaaaacg gcagaattta gagagcgttt gaacaaagag gagacacttg acgcgctttt
     1441 accggaggca tttgcggcgg tgagagaagc ttcaaagcga acactcggcc agcggcattt
     1501 cgatgttcaa attctaggcg gaatcatttt acacaagcgc ggaattgctg aaatgcgcac
     1561 cggtgaggga aagactctcg tggccacact tccggcgtat ttgaatgcgc tcggaggaaa
     1621 aggggttcat attgttacgg tgaatgatta cctgtctcgc cgcgacgccg tgtggatggg
     1681 acaaatttac gcctatcttg gaatttccgt gggcgttatc aatcacgaca cttcttttct
     1741 gtatgatcca acgcataaag aattggataa agagcgggac gaaatcgctt catttaaagt
     1801 ggtttatgat ttcctgcgcc caagtacaag aaaagaagcg tatgaggcag atattaccta
     1861 cggcacgaac aatgaatacg gtttcgacta tctgcgagat aacattgaat atgatatttc
     1921 agatttgcgt cagcgtgaat tcaactatgc aattgtcgac gaaatcgatt ctattttgat
     1981 tgatgaagcg agaacgccct tgattatttc cgcgcctacc actgaatccg aaaatctata
     2041 tgcggtgttt gcgaaaataa gcgagcggtt gaataaggac gaggactata ctgttgacga
     2101 aaaattcaaa tcgatcgtgc taactgacaa aggaatcgaa aaagccgaga aagtcctcgg
     2161 agtcgataac atttacactg aaaaaggaat taaatacgtt caccatctgg aaactgcagt
     2221 tcgagcgaag gcgcttttcc ataaggacaa agaatatgtc gttaaagacg acgaggttat
     2281 tattgtcgat gagtttacgg ggcgactcca gccgggacgg cgctggtcgg aaggtttgca
     2341 ccaagctatt gaagcgaaag agggcgcacg tgttcagcgc gagagccgga cctatgcatc
     2401 cattaccttt caaaattatt ttaggctcta caagaaactt gcaggtatga ctggaacggc
     2461 gctcacgtct aaagaagaat tcttgaaagt ctacgggctt gatgtgcttg aaattccgac
     2521 caataaaccg atggcgagag cggatcacaa cgaccttatt ttccagactg aaaaaggaaa
     2581 attcaaggca attgcaaaaa aagtcaaaga actgaacaaa aaaggccagc cagtgcttat
     2641 cggtactgtt tcgattgaaa aaaacgaatt acttagttca tatttaaaga gcgaaggagt
     2701 tgagcacgtg ctgttgaacg ccaagccgca gcatgccgaa aaagaaggag aaattatcgc
     2761 ccaagcgggg cgaaaaggtg cagtcactat ttcgacaaac atggcgggac gaggagttga
     2821 tattaaactg ggtggaaatc cggcaacgac cgagcagtac gaaactataa aaaacaacgg
     2881 aggacttttt gtgcttggaa ccgagcggca tgaagcgcgc cgcatagaca atcagcttcg
     2941 aggacgttct ggtcgtcagg gagatccggg cgagacgcag ttttttgttt cgcttgaaga
     3001 tcatctcatg cgtgtttttg cctctgatac gataaaaaag ctaatgggtc ggcttgggat
     3061 tgcggaggat gtgccgattg agaaccgtat cataacaaga tcacttgaaa cggcgcagac
     3121 aaaaattgaa ggcttcaatt ttgattcgcg aaagcacgtt ttggaatatg acgatgtttt
     3181 gaatatccaa cgaaaaactg tctattcacg acggaggaaa attttaacgg gagctctcga
     3241 cgacattgaa gaagagctca tgctaatagt cggagacgat gagaaactat tcaaagtaat
     3301 cgaggaaaaa gaaaaattac tcggcgaaga agaattttgg aaggcggtta agcgtctttt
     3361 tctgcaggcc attgatatgt tttgggtaga ccatctcgaa atgatggatt atctgcgctc
     3421 gagcgtcagg ctccgcgcgt acggccagcg tgatcctctt gtcgaataca agagagaggg
     3481 actcgggctt ttcaaggaaa tgcaggaggc tacgaacgca caaatactcg agatgttgcc
     3541 aaacatcgga gcaggcgcgt ttaaaaatga ggaacagcgg gctcgcgaag tccaaaaaca
     3601 aatgaaactt attacggacg aggccaatca aggtcggacc ttaaatcagc aaaaaggtcc
     3661 gaccttagca actccaagcg gtgaaaaaat cggccgtaat gatctatgtc cgtgtggaag
     3721 cggtaagaaa tataaaaaat gcggtttaat caacgcgtcg ttccataaaa agtcttaaaa
     3781 aatttatcac gctaagaagc aaacttctag tacttggatg taaaaaattt catggcatat
     3841 aaaccgcaat caatcttgat tgaagaagtt tctgacgagc aattgttggg gggttatgat
     3901 gctcgtgttg aggagctata tttagaaaag gaaaatttcg aaagaaatgg aattactcgc
     3961 cgaataggcc attatgagtt tgatgagact attaattcga ttagcaaaga aattgaaagg
     4021 acaaagcgtc tgaggaatgc attgatagaa cgaatgaaac tgaaaacgaa gaaacagatt
     4081 tggtacaaag atccggatat tctcgtaaaa ataatacttg ctttaatagg cgcgttggca
     4141 acagttatcg cggcttggat agttagttct cgtaaatgat tatgaatgaa agagacaatc
     4201 taaaaagtgt cgttggaaat aaaattagct aatgctcaac ttactaaaag aaattgtaat
     4261 ttatgatacc gagtacacaa catgggaggg tgcgcaaaaa cgcaattgga gcggccccgg
     4321 agaataccga gaagtcgtaa atataggagc cataaaaata gagacagaaa attttagagt
     4381 gttggatagt ctcgatctat ttatcaagcc gtttaaaaac cccattcttt ctaaatattt
     4441 cataagcctt acttctataa ctcaagagga agttgataca aaaggtattt cgcttaaggt
     4501 ggcaatggga gaatttgtaa aatggtgcgg taggcttcaa ctttactcgt atggacctga
     4561 tcatgaaagg ttgattgaaa actgtgctct gattgatatt cctttcttgc ttaaaaaaga
     4621 aaactgtcac gatgtgcgtg atatttttcg aatgcacggc attcccgccg aaaattatat
     4681 gtcgagcact attgtgaggg catttgggca ggagccgact cgccaggccc atacgggagt
     4741 tgatgactca cgcgctattg ctgatgcgct tagattgttg aaactaaggg aacattaagg
     4801 acattttgtt cgttataatt aacagtggat ctctggtggt ttcgacaatt ccataatata
     4861 attttgctta tgcttcgaaa atgtcgaagg tgtggcgctg gatttgagcc gcatcaatgg
     4921 aatcacgttt tttgcggttc taaaattcaa caaacgggct gctcttggtt aaatttaact
     4981 tcggacagaa gcaaacgacg ttggcaaaac gaaaaatacc gcgcatatca aaaagaatat
     5041 ggcaaaattt ggaaaagaat acagcggaaa ttgaatgcgg attatgccgt cagacagagg
     5101 ttacttaaaa gggaggcagc tttaactccc gaagggaaaa aggtggcgca agcctggcgc
     5161 agaagaaacc ttgttaaagt tttattttgg aataagaaaa gagcaagaat caaacgtgga
     5221 gttgttggga gccatacttt tgtagattgg gtaagtataa agagtaaata taattattgt
     5281 tgtgccatat gcaagttatc cgagagtgat ctccgtaaaa aatggaaaaa tactcacttt
     5341 acagaattaa cgatggatca tatattacca atatccaaag gaggtacaga ttatataaca
     5401 aacattcaac ctttatgtat ttcgtgtaat gcaaagaaaa gagatgccta agaaaaaaca
     5461 aattgttgtg gcggtttcgg gcgggatgga ccctctccat attggtcatg tgcgtatgct
     5521 agtggaagca aagaagctcg gcagtaaatt aattgtcatc ttgaataatg ataattggct
     5581 gatgaagaaa aaaggatttg tctttatgaa agacacggag cgcaaggaag tgatcgaaaa
     5641 cataaattca gtggatgaag tttaccttac agaacatgcg ccagatacca ccgacatgag
     5701 tgtttgcgtt gcgctcggta aaatcaggcc tcatgttttt gccaatggag gagaccgaaa
     5761 acccgatggt gatccggtgc ctgaagtgga gatctgcaga gagctcggca tcaaaatggt
     5821 ctacaacatc ggttccggag gaaaggtaca gtcaagttcc tggttggtca aaaaatatct
     5881 cgaaactcaa aataagaaga aataggcacg ctttgactat accggctgga caggggaaat
     5941 aggcgcgtgt actatgatcc attaagacat gacccataca tacattgctg gtggagtcgt
     6001 actcaataaa aagggcgaag tccttattgt taatcagctc ggtctttcgt ggtcattgcc
     6061 gaaaggccac attgaagaag gcgaggatga acttgctgcc gcaaagcgtg aaatttttga
     6121 agagtcagga atttcacaat tatccgtcat aaagcggctc ggaagctacg accgggcacg
     6181 gatcgcgctc gatgttacga aggaagataa acgtgaaatc aggactatta ccctttttct
     6241 cttcacaacg ccgcagatga cgttggagcc ccgcgatgcc gaaaacccag aggcccgttg
     6301 ggttcctcga caagaggtga cggattatct aacacatcga aaagaccgtt atttttttaa
     6361 gaatattatt ccagaaatag acgaactctt cccgccaaaa tgaatcccgt tttggcgggc
     6421 ttttgaaatt agattaactc ttataaaaag aaagatattc cgtgtgtttc cgagagaaac
     6481 aaccacacac taacattaca cgatcgtgtg gtgttagtgt attcattttt gttctggaaa
     6541 tttggacata aggttaactg tacatcacct cataagggta accctgcaag tacatgatgt
     6601 ttttatggtt tttgacacta tatcttcgaa acagagtaga cggcaaaacg cgaatagtta
     6661 gggttgtgtc tggagccatg cgcggtacgg ccggtgctca ccattggggt gggcaccggc
     6721 taaatttttt tatcgcctca gtgtcattgg cttttcttgt cgtgctgttt aatctacaat
     6781 aagtgccttc gcttgctgta gtcggactga atgcagcaat gcggccacgg ccggcgtctc
     6841 cttgggttag gaggcgccgg ctaatcattt cctctttttc gagctgtagt caaccaactg
     6901 agcgagtatt ctcatttgag aatgttggat tttcttattg ttagagtgaa acttgttttt
     6961 ttcaaaacat tgcggagcac gacggtgtga gcatagcaat taaccagcaa gttaatatgc
     7021 gtgttttgaa aaagatcaag ttgaacggtt tttaccaact tgctcgactg gcgagtccga
     7081 gcgcatactc tgtccaccat acacctgctg tcggtccacc gtggcacgtt ccgtcagatt
     7141 cgcctggttt ctttagccag agtagtgcat cgatgagcgc gttgttcgtt tgtgatgttg
     7201 gcttttggcc aagggcgcgg ccactcggat tgcaccattc accgtttgac cccgctccgt
     7261 tgcgcgatgt atcgataata aaatgtttgt tcccgatcaa tctcgaaatt gcttcaccat
     7321 atcgaatatt gtctgctgtt gtgtagaagt ttgagatatt tattgcgaat ccatcggcct
     7381 ccgcaacatg cgcggactgc agacggggag cgatggtttc cgccggaagc catcctgggt
     7441 ttcctgcgtc aacatacacg gtgatgtttg gttttgcttt aagtgtgcgg actgcatagc
     7501 cgatgaggtc aagtcgatcg ctctgaagcg agggggaaag gcaggtgatg tttgacaatg
     7561 cgtcaggttc gataaccacg gccgctttgt tgttgccgat agtatttacg atcgaattta
     7621 tccaatctct gtatgctccg cttgatccga caccacctgc agaatagccg ccacagtctc
     7681 ttccagggat gttataggca ataaatacgg gatattcccc ggcactctgg gctttcgaga
     7741 taacactgct gatatcctgg ggaatatccg accaatctcc gagccaaact gactctgcgc
     7801 cgctggcaat ttttgacatc aagtttgcgt ctgctggtct tgaatttttc cactcgttta
     7861 cgaattccgt ggcttttgag tctttattga cgtaaaacct cacgccgtct aggggattag
     7921 aaggagcgga agtggggggc ggtggaactg gttttggatc tggtgttgcc gtcggagcag
     7981 gaacaggagt aggtgccgga gacggattcg aagtcgaatt ctcctttgga tttggcggtg
     8041 agtcgggaac cggcgtcgat cgcggtgcag gtgcaggagc cggagttggc gtcggtgaag
     8101 gagagggtga agaagcggga gcaggaatag gcgatggagt tggcgaatcg ccgccgttaa
     8161 tttgatttat cacatgcgac aatattctct cacgaagttg ttgagaagga gaagccgccg
     8221 tgtctgctgt tcgtgatgat tccgatgtgc tcacaacttc attttgactt gtgtcaggtg
     8281 cgcttactcc gtctgtgttg ctttcactgt cgctcgtttt cgaagcattc gtcttgtttt
     8341 ctactactat gtgaattgtt ctctcttcaa cgatagttcc actttgctgc gcaatgaatt
     8401 ttatttcgta tggtcggctt tcgtgccacg tccagttgct aacgtcaaca cctgcttctt
     8461 tgtgcggcca atcgcgctca ttgttttcca ttttattcca tttgccgtta tcgacttgcc
     8521 aaaacatctc ataatttgag acgtcagtgt cacgtaagag cgctttgaaa ggctgcgcgc
     8581 cgtctatttt tgcgttttca acgggccacc atacgtcaat tgaagacgct gcgttgagaa
     8641 agctaccgtc accgataaaa gggcttattg tagatacata ggtaaataca agtagtgtta
     8701 gtccggctgt aagaaaccgg aataatgtgt tttttcgata tgccataatt attttttgtt
     8761 gccgcctgtg ttttcataac tcatccgttc aggataacaa cgagcattat acttcggcac
     8821 tcattcgttg caatcttgac agttatgcgg ctcaaccacg cccgcaaaat cggttccgat
     8881 ttttttacaa atattttcgt acttcaaatg ttggtaaaat ccttgcataa taccttgcat
     8941 aatacattga aatgtatcaa tatataatgc agaaatagaa ttccgcgccg attctcctcg
     9001 aaatgtcgac cgaacagcct tcgcgccgta aacattaatg ttaggccgag cgaaataatg
     9061 gtgaggcggc tttttttgtc cctcaaatag ggcagagtac agccattttt ggtcacctcg
     9121 aaaaaggcac gtttacatga acagaaaaac attttgaaaa tattttttta ttacttatcc
     9181 acagagcctt ttcgataggg ctttgtaaaa tggagtctcg atattattaa tgctcggcaa
     9241 tttatcacta aagaattatg gcgaagaaaa cagttgaatc gaaaaaggtt gaatcagcga
     9301 ttgaattggt aaagaaaatc caaaagagag atggttcggt agttgcattc gatttcgcca
     9361 aaattgtgcg cgccatccac aaggcaatga tccaaacggg agaagggtca gaaaaagaag
     9421 cagaacttgt tgcaaataaa gtctatgccg agctcgtacg catcgcgaaa aaatttaaaa
     9481 ccttcgttcc gactgttgaa ggtatccagg ataccgtaga aaaggagctt attttgtccg
     9541 aatacgtaaa aacggcaaaa tcatacattt tatacagaga aaaaagggca aaattacgtg
     9601 aaaaaggcct gcaggtaccg gttaaagtta agaaattagc agaagagagc aaaaagtatt
     9661 ttagaaattc acttggagag ttcgtctact atcgaacgta ctcgaagtgg atcgttgaag
     9721 agaatagaag agagacatgg attgaaaccg tcgaccggta tattgacttc atgaaagaaa
     9781 atcttggtgg taaattgaaa gatgccgagt acaaagaaat tcaagaagct attttgcgcc
     9841 atgaggcgat gccttcaatg cgattgttgc agttttcagg caaagccgct cgttcaacac
     9901 atgtttgcgc atataattgt tcttacatcg caccggaatc ttttcaggat ctcgccgaga
     9961 tcatgtacat ctcgatgtgc ggcactggag ctggctggtc agtagaaagt cagaacgtac
    10021 aaaaatttcc gcagatactt ccgcaaacgg gagaaaaatt gccgacgcat gtaattcctg
    10081 acagtaaaga aggatgggct gactcactca ctctcggaat gaaaacatgg gctttaggta
    10141 aagacgttga atttgattat tcagaacttc gactggctgg cactcggttg aaaaccatgg
    10201 gaggaaaatc ttccggtccg gatcctctgc ggcgattgct gacatttaca cgcgaggcca
    10261 tgcttatacg ccagggcaag cggcttcgaa accttgatgt gcacgacatt atttgtatga
    10321 tcggagactg tgttgtcgca ggaggagtac gccgctcggc aatgattagt ctctcggata
    10381 tggatgatga tctcattcga gacgctaaaa agggcgcttt ttatgtcaca catccgcacc
    10441 gatcgctagc caataattca gctgtatacc tcaaaaagcc caccaatgaa gagtttttga
    10501 aggaatggat ggccctcatc gagagccgtt caggtgagcg cggtatcttt aatcgcggat
    10561 cgctcgcaaa gacgcttccg gcgcggcgaa tcaaagtgct taaggagcac aaaggatatt
    10621 ttgattcgac gggtgagatg atcatcggct caattggcac aaatccgtgc ggcgaaatta
    10681 ttcttcagtc aaaacaattc tgtaatctgt ccgaagttgt tgccaggaga gaagataccg
    10741 aagcaacgct tcttcgaaaa gttcgcgtcg ccactatttt gggaacatac cagtcgtcgc
    10801 ttaccaactt tccgtatctt tcaaaagagt ggcgtgaaca ctgcgaacgc gaacggttgc
    10861 tcggagtttc aataacaggg cagtgggatt cacctgtagt tcgacaaccg gatgttctga
    10921 aaaaacttaa aaacgaatcg ataaaaatta acgaacaata tgcaaaacga tttgggatca
    10981 gcgcttcgac atgtatcacc tgcgtgaagc cgtcgggtac actttcgcaa actgtcgact
    11041 gttcttccgg tatgcatcct cgtcatgcgc cctactacat tcgtcggatt cgaatttcaa
    11101 cgactgatgc gcttttcaaa atgcttaaag atcaaggagt accatttcat cctgaagtag
    11161 ggcagccagc ggaaaccgcg agcaccttcg ttcttgaatt tcccgtcaaa gccccagaag
    11221 gttcaatcta caaaaatgac attaccgcca tcgatcagct cgaacactgg aaagtcgtga
    11281 aagtaaatta cacagaacac aatccttcag taacggtgtc ggtcggcgac aatgaatgga
    11341 ttgatgtggc caattggatt tacaaaaact gggatatcgt gggcggtctt tcattcttgc
    11401 cgagggataa ccatgtctat caactagcgc cctatgaacc gatagatgaa aagaaatata
    11461 aagaactttc aaagcggctt gcacatattg attactcgaa aattattact tacgaacggc
    11521 aggacgaaac ggaagtgaag aaagagttgg cgtgtgtggc aggggtgtgt gagattgtct
    11581 agaaaaataa gcgacagcga ggtcgctata tttttcttga tcccgtacta attttgccgc
    11641 tatgtttggt aaaatattgt ttagttgcga ttgaaaaact gaaaaatcaa agcgcgtgcg
    11701 cgaatagcgt ttcccttatg ggcgaagcgc tttgatgtca tttaaccgcg caaaatttag
    11761 tactgggatg taaggagaaa cgtacagctc ggcgcatttc tttgtcgctc gttccgatcc
    11821 tcatacgtat cccttagaga catacaacga ttccacgcta tagcgtcaaa tcattgtaca
    11881 tgtattttta gtggtaatta tgtaattggg ggatacatct gccgatcctc accttgctgt
    11941 actccaacta caggtcggct cagttctcac tcgttcctcg aattgcatcc aatctgtgcg
    12001 tctctctgtt tctggaatcg taaaacagat tttagtaatt actttgaatt acaaaactct
    12061 ctcacctcat cttttttaat ccatctcaaa caaccgcagt cgtccgcagt tctcgcaaaa
    12121 gtcagttttc a
//
LOCUS       DATDSU010000130         7873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1128759, whole genome shotgun sequence.
ACCESSION   DATDSU010000130 DATDSU010000000
VERSION     DATDSU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128759"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(853..1074)
                     /locus_tag="VI981_04535"
     CDS             complement(853..1074)
                     /locus_tag="VI981_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479590.1"
                     /translation="MMVYAVVRACDESGERTMLFRSFTTSKQKAEERALRLDQQYGPH
                     HYFFVVEDEVGDDFELPDEHSDDEVMSWY"
     gene            complement(1448..1708)
                     /locus_tag="VI981_04540"
     CDS             complement(1448..1708)
                     /locus_tag="VI981_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479591.1"
                     /translation="MKEMYLMEVFKKLGRREYKLRYCGKSADGLAFELETDIGGTLTE
                     AKKFVLPISQRALIGKAVLIEVSHRAYSSGGSRNERGQRIEI"
     gene            2140..2910
                     /locus_tag="VI981_04545"
     CDS             2140..2910
                     /locus_tag="VI981_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4479592.1"
                     /translation="MKIEEGLKNLGLAEKEAKVYIALVQLGRGSAQSVADESGIKRPT
                     AYVILENLIDKGMAVKIPRARKKLYEAKPPDEFFAEAEERFALSKTILPELMAMADKT
                     GPKFKTLYYEGEQNVKRMLSGINKKMAGKEILGFYARETENISSEMNEFFHQWNEDCK
                     KNGITVRGLTPDDPSLEWYRKRTEYFGHVFKYLNMKDYSSECSIEIGDDFIQIFSLRY
                     MQGVYMENPDVARTLRQIFEMVWAARPEKIEGALSEDK"
     gene            complement(2978..3379)
                     /locus_tag="VI981_04550"
     CDS             complement(2978..3379)
                     /locus_tag="VI981_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479593.1"
                     /translation="MNKKNSAKSFSEELRHLEKIAKKMMACKAAAEEKERLQRIDSTI
                     NGYLSELCLTEKRLKEKMLKAASRGKKEAFFTTFEENSWVFLPHCLEALNRELANRQE
                     MFSKTLGTPVTLFLESFDPYSPARICAKWKV"
     gene            complement(3406..3849)
                     /locus_tag="VI981_04555"
     CDS             complement(3406..3849)
                     /locus_tag="VI981_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479594.1"
                     /translation="MSKREKHEKPNEDLLVKLRSGQLISWRGTDEYGFPDRRYGVVRL
                     VEENVIQIFDGGKLISFSKQELKTQKVRSLTPNNAVKTLNDRVSTNTLSSMLSQARSE
                     SYSEGESRAEAEYSRARSEAYERLPQEEKNRIRWEQERASDRLVN"
     gene            complement(3842..4468)
                     /locus_tag="VI981_04560"
     CDS             complement(3842..4468)
                     /locus_tag="VI981_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479595.1"
                     /translation="MQGNSLIFCGPARERTYEEKISDGVTLILDNHILAFVDYLCKTT
                     HANWKFLRTGEKERGLNVCNLSIELKYDKSFLLRAELFSDKDPIVSIVIGGKIFNKSL
                     YASDFECSRYCQSGSVLKKLEKLYWKLLAEKEGKVLRIRKQRKSDLGKCTKEKHEMEL
                     DTIREKVRVALKVEADSAERSSIGYDDYDDFDFITTEDDNDEEEDTDE"
     gene            4627..5460
                     /locus_tag="VI981_04565"
     CDS             4627..5460
                     /locus_tag="VI981_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4479596.1"
                     /translation="MDASPKLFEEFGLTEKEGGVYLTLLKLERAKVSTLGKKSGFPRT
                     SLYPILEKLLKRGFVRILRIGNHQEWEASEPKEIYKQAKETIKGLEEAVPLLEALKGS
                     LAGHIRTADILFYKSIDGIKKAYDDILKLRRGERVYSIEGNFSQPSKLRRFRKSYMLE
                     WLAKHKKTEVIYEAVESEKLFQLIETIDLELLKGQAGRLVVLTILPDHLMNFDAEIFC
                     FRNTLVIVIVSQDTAIFIHNPEVVEAFRSLFVIAQTLGKKVDFNRAVFEELARRGLKK
                     E"
     gene            5515..6237
                     /locus_tag="VI981_04570"
     CDS             5515..6237
                     /locus_tag="VI981_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4479597.1"
                     /translation="MVAGSQTVALILGGTSGLGLELARESERNDVKPMLLGRSKSLEF
                     TGDSHVYVDLADKHRVYALCQYIEESYIIPYAKYFFWNAAMFERASITDMCDPKKVIE
                     VNVVSPTKIIESLIRRKKSLNLPVHIVAVSSTSSWRARADEAVYCGTKAYQSQFMRSL
                     GLELMRDLPGSKVTIVHPSGMKTNLFNGMKTDTSKFMDPAGVAKIIWSEVLKQDLAID
                     EFNVDCVNSEPVLVRESRIPLA"
     gene            6249..6926
                     /gene="pyrF"
                     /locus_tag="VI981_04575"
     CDS             6249..6926
                     /gene="pyrF"
                     /locus_tag="VI981_04575"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY4479598.1"
                     /translation="MADNKPEIIIALDGMEVNEALHMARLLTGKAWGFKVNDLLLEAG
                     GLIITRLKKYGHVFADAKLHDTPNTVFNSVKKLSRAGADFISIHASADMAMLLAAQKA
                     AGQSNLVGISVLTSNENFSKSTFKKAVFQSVVSRAYGIVCSPCDMKLLRKCPGSNALK
                     VISPGIRPLGMEVAEDDQKRIATPADAAQAGADYIVVGRPVTGAQNSKEALDKIIKEL
                     EEMKTPQ"
     gene            complement(6959..7237)
                     /locus_tag="VI981_04580"
     CDS             complement(6959..7237)
                     /locus_tag="VI981_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY4479599.1"
                     /translation="MASINDIPKGKGKIVNIGGKDTAVFNDNGTVKAFYTKCPHAECH
                     VNWNDKENTWDCPCHGSRFENDGTLKKGPSTQGLYPMDIKMDGGEITR"
BASE COUNT         2088 a         1761 c         1779 g         2245 t
ORIGIN      
        1 ttgacaggcc gcaaaggatg gcgcgcgagg tggagggact cgcatgcctt ctgttgccac
       61 gccgtcctca cggcgccgcg gtaagctgcc attctatccg gtcacccgaa tagagatacc
      121 gagaagccgg gtcaggtagt cgcctacctg caccgtctcc gcggggagct cgactgcgag
      181 atagaggccg tttccggcac tctcccaacg ccatgtggcg ttggtttccc gcagacgagt
      241 ctccaccgtc cgtatccgct gtgagcagat aggacagtgt tggcaagccg ccaacgccac
      301 ttccatcatc aaggtgttac cctcgatgac ggtgtgacgc tgattgcgcc atcctgctac
      361 tgaaaggtag cagtcccgtc gagccatgct atcacctcct ttaaaggcga cattccccca
      421 agacttatct actcaccaac ttcaccgcgc cctcgcgcgt gctgaaatca gttttcgaac
      481 tcggagtacg tttattgcaa ttcaatagtt acaggcggct cgtccagaga atcacttttt
      541 ctttactttt ttacgtttac gaggcagaga tatgtgattc cagccagccc aggcatgagg
      601 gaaattgcgt cgtttttctg cgtgttttgt gcaaaagaga aacgattctt tgaaagactc
      661 aagcttgaga taccactcgg ccatttcgta gcagtcgtct tcgcagcatc gatctctttt
      721 atcaatcacg gcgaccctcc tttttgtttt gtttgtcaat gttcgctcca tgcgcttccc
      781 gccaaaactt tcagtatcct gaaaactttg gtaggggccg aatgtttttc attcggccgg
      841 tggatactat tatcagtacc aggacatcac ctcatcatcg gaatgttcgt cggggagttc
      901 gaaatcatcc ccaacttcat cctcgacgac gaaaaaatag tgatgtggcc cgtactgctg
      961 gtctagcctc agagcccttt cttccgcctt ttgcttactg gtggtaaagg acctgaagag
     1021 catggtgcgc tcgccggact cgtcgcatgc gcggacgacg gcgtagacca tcatggttgg
     1081 ctcctttcgc ggttaatacc gcgtatgaat gaaagaactt ttgagcgaat tatttcgccc
     1141 gtcaaaacgt tcagtatcct gaaaactttg gtaggggccg aatgtttttc attcggccgg
     1201 ttaaaatagg gcaacgtact tctggtccct tatcggtttc atggcgaggc gcccccgtaa
     1261 cgccccactc cactatccgc ttgccgcgga tccgattccg cccccgcccc ggggtcacgg
     1321 tttctcgagt aggtatccgg caggacacca gagcggggaa gcaaaccccg cctctcgaga
     1381 tcctcgccca cggtcggggc ttggaggaca aagcgtgaaa ttgttcggtc agatattctg
     1441 cacaacctta gatttctatc ctttgtcctc tttcattgcg agaacccccg ctactatatg
     1501 ccctgtgtga gacctctatg agcacagctt tcccgattaa tgctctttgt gatattggga
     1561 gaacgaactt cttggcctct gtaagggtgc cgccaatatc tgtctctaat tcgaatgcga
     1621 gcccgtcagc actctttccg caatatctca gcttatattc tcggcgacca agttttttga
     1681 aaacttccat caaatacatc tctttcacag aacactcaag ggtgtaaaaa acatagtcac
     1741 tgtcgcttcg aaacggccag ggcatgttct ttcctcactt gttgcaatat tctagactta
     1801 tcataccacc agatgccata tctgtcaata attatactta tgataatata gtgaaaagtg
     1861 catttttata tagattttac taactatttt taatatttta cttttatttg tcaaaattat
     1921 tgacaaatta tttaagtgag aatatactaa atgtatgggt atgaccccag caccaaattc
     1981 cgaaaatgcg ttagtggttt gcatgagtag ttctcgcggt accacctccg ccgagcagag
     2041 ttcggctgcg gcttacaaaa agctaagagt ggcttgtatt aaaaattgat gcggggttca
     2101 taatttatac ctaaattttt aaaaaaattt agacaaatta tgaaaattga agaaggactt
     2161 aaaaatttag ggttagcgga aaaagaggct aaggtttaca tagcccttgt tcagctggga
     2221 cgcggttccg cacagtcagt tgccgatgaa agcggcatta aacggccgac ggcatatgta
     2281 attcttgaaa atcttattga caagggaatg gcagtaaaaa taccacgggc acgaaagaag
     2341 ttatatgaag caaagccacc cgatgagttt tttgccgaag cagaagagcg gttcgctctc
     2401 tcaaaaacca tattgccgga acttatggca atggccgata aaaccggccc gaagtttaag
     2461 acgctgtatt atgaaggcga gcagaacgtg aaacgcatgc ttagcggaat aaataaaaaa
     2521 atggcaggaa aagaaatatt gggattttat gcgcgcgaga ccgaaaatat ttcgtccgag
     2581 atgaatgaat ttttccatca gtggaatgag gattgcaaga aaaacggtat taccgtgcgc
     2641 gggcttactc cggacgaccc gtctcttgag tggtatagaa aacgcactga atacttcggt
     2701 catgtattta aatacttgaa tatgaaagac tactcgagcg aatgctcaat cgagatcggc
     2761 gacgatttta ttcagatttt ttctctccgg tatatgcaag gcgtttatat ggaaaatccc
     2821 gatgttgcgc gtaccctccg ccaaattttt gagatggttt gggccgcgcg tcccgaaaaa
     2881 attgaaggag ctttgtcaga ggataaataa aggtaaaaaa tactatcatc cgcccaaata
     2941 aaaaataacc gccgagataa ttctcggcgg cctcttttta caccttccat ttcgcgcaaa
     3001 tgcgagcagg tgagtaggga tcaaaagact caagaaacaa agtgactgga gttccgagtg
     3061 tcttactaaa catctcctgc cgattggcta gttctctgtt aagcgcctca agacaatgag
     3121 gcaagaatac ccagctgttt tcttcaaatg ttgtgaaaaa agcttctttt ttgcctcgag
     3181 aggccgcttt gagcattttt tccttcaacc ttttttctgt gagacacagc tctgacaaat
     3241 aaccatttat ggtgctgtct attctttgta aacgctcctt ctcttcagca gccgctttgc
     3301 acgccatcat cttttttgca attttctcta gatgtcggag ttcttcagaa aaactttttg
     3361 ccgaattctt tttgttcatt tttgccgtct ccttttcggt gtctgttagt tcacaagcct
     3421 gtcggatgct ctttcctgct cccagcgtat gcggtttttc tcctcttgag ggagtctctc
     3481 atatgcttcc gatcttgccc ttgaatattc agcttcagcc ctactttcgc cttcgctgta
     3541 gctctccgag cgggcctggg aaagcatgct cgagagtgta ttggtagaaa cacggtcatt
     3601 caatgtcttt acggcattgt tcggagtgag cgaacgaacc ttttgtgttt ttagttcttg
     3661 ctttgaaaaa gaaatcaact ttcctccgtc aaaaatctga atcacatttt cttctaccaa
     3721 ccgcacgacg ccataccgtc ggtcggggaa accatactcg tctgtgccac gccacgaaat
     3781 gagctggccg cttcggagtt ttacgagaag atcttcattg ggcttttcgt gcttctctct
     3841 cttactcatc tgtgtcctcc tcttcgtcgt tgtcatcttc ggtggttata aaatcaaagt
     3901 cgtcatagtc atcatatcct atgctacttc tttcggctga atcagcttcg accttaagtg
     3961 caacccttac tttttctctt atcgtgtcta gttccatttc gtgtttttct tttgtgcatt
     4021 ttccaagatc gctttttcgc tgttttcgta ttcgtaagac ttttccctct ttttctgcca
     4081 gaagtttcca atagagcttt tctaatttct ttaacacgct tccgctctgg caatagcggg
     4141 aacattcaaa gtccgatgca tagaggcttt tgttaaaaat tttacctccg ataacaattg
     4201 aaactatagg atctttatcg gagaaaagtt ccgcgcgaag taaaaaagat ttatcatatt
     4261 tcaactcaat tgaaaggttg cagacgttga gtcccctctc tttttcgccg gtgcggagaa
     4321 atttccaatt ggcatgcgta gttttacaaa ggtaatcgac gaaggcaaga atgtgattgt
     4381 cgagaatcag cgtaacgcca tcactaattt tttcttcgta tgtgcgctct ctcgccggac
     4441 cgcagaaaat caaggagttc ccctgcacta gtttttaatg ttctatgcta tcattataaa
     4501 ggaaaaataa tatattgaca cagttttata tattatttat acataatatc ataatgtata
     4561 gtaaaatcag tattatataa tgaattaagt tgactaaaat atcgtcatat tataatatta
     4621 aagcatatgg atgcatctcc aaaactattt gaagaatttg gattgaccga aaaagaaggg
     4681 ggagtttatc tcacactatt gaagcttgag agggcaaagg taagcactct tggcaaaaaa
     4741 tccgggtttc caagaacttc actttatcct attctcgaaa aactccttaa gcgcgggttc
     4801 gttcgaattc tccgtattgg gaatcaccaa gagtgggaag caagcgaacc gaaagaaata
     4861 tataagcagg caaaagaaac cataaaagga ttggaagagg cggttcctct tcttgaggcg
     4921 ctcaaaggaa gtcttgctgg acatattcga acggctgata tcctctttta caaaagtatt
     4981 gatgggataa aaaaagcata cgacgatatt ttaaaactta gaagaggaga acgcgtctat
     5041 tcgatagagg gaaacttttc tcaaccgtca aaactaaggc gatttcgaaa gagctatatg
     5101 cttgaatggc tcgcaaagca taaaaaaaca gaggttattt acgaagcggt agaaagcgag
     5161 aaactctttc agcttattga aacaattgac ctcgaacttt taaaaggaca ggccgggcgt
     5221 cttgttgttc ttaccatcct tccggatcat ttgatgaact ttgatgctga aatattttgt
     5281 tttcgaaata cgcttgttat tgttatcgta agccaggata cggcaatctt tatccataac
     5341 ccagaagttg tcgaggcatt ccgaagcctg tttgttatcg cgcagacact cggaaagaaa
     5401 gttgacttta acagggctgt tttcgaagaa ctggcacgga gggggttgaa aaaagaataa
     5461 aagtatgcaa caaatcttag tatcctactt atcttcatat acatcgaggg aacaatggtt
     5521 gctggaagcc agacagtcgc actgatactc ggcggaacat cgggacttgg gcttgagctt
     5581 gcgcgggagt ccgagcgaaa tgacgtgaag ccgatgctgt tgggaaggtc aaaatcgctt
     5641 gaatttacgg gcgactccca tgtgtatgtg gaccttgctg ataagcaccg ggtgtatgct
     5701 ctttgccaat atatcgaaga atcttatatc attccgtatg cgaaatattt cttctggaat
     5761 gcggcgatgt tcgagcgcgc ttccattacg gacatgtgcg accccaaaaa ggttatcgaa
     5821 gtgaatgttg tgtctccgac gaagataatc gagtctctca tccgccgcaa aaagagcctg
     5881 aatctgcccg ttcatatcgt tgccgtttcc tcgacttctt catggagggc gcgggcggac
     5941 gaagcggtct attgcgggac gaaagcatat cagtcccagt tcatgcgctc gcttgggctt
     6001 gagctcatgc gcgacttgcc cgggtcaaaa gtaaccattg tgcatccttc cgggatgaaa
     6061 acgaatcttt tcaacggaat gaaaacggat acgtcaaaat tcatggaccc tgcgggtgtt
     6121 gcaaaaatta tttggagcga agttttgaag caggaccttg caatcgatga gtttaatgtt
     6181 gactgcgtca acagcgagcc ggtgctcgtg cgcgagtcgc gcattcccct cgcttagagg
     6241 acgagcggat ggctgacaat aaaccggaaa tcattattgc gcttgatggt atggaggtaa
     6301 atgaagcttt acacatggct cggcttctta caggcaaggc gtggggcttt aaggtgaacg
     6361 atcttttgct tgaagcgggg ggtttaatca ttacgcgcct gaaaaaatat ggtcacgtat
     6421 ttgccgacgc aaagcttcat gacacgccga acacggtttt taactccgtg aaaaaacttt
     6481 cgcgtgccgg agccgatttc atcagcattc acgcgtcagc cgacatggca atgcttcttg
     6541 ctgctcaaaa agcggctgga caaagcaatc ttgtcggcat aagcgttctc acatcaaacg
     6601 aaaatttttc caaaagcaca ttcaaaaaag ctgttttcca aagcgttgtg tcgcgcgcgt
     6661 atgggattgt ttgttctccc tgcgacatga aactgctccg gaagtgtccg ggaagcaatg
     6721 cccttaaggt aatttctccc ggaattcgcc cgttgggtat ggaggtcgca gaagacgacc
     6781 aaaaacggat cgccactccg gctgatgcgg ctcaagcggg cgcagactat atcgtcgtcg
     6841 ggcgtcctgt aaccggagct caaaattcta aagaagctct cgataaaata attaaggagc
     6901 ttgaagaaat gaaaacgccc caataacatc agggcgtttt tttatttttt gtaatttttc
     6961 atcttgtaat ttcgccgcca tccattttta tgtccatggg gtaaagccct tgcgttgaag
     7021 ggcctttttt gagagtgccg tcgttctcaa agcgcgaacc gtggcacggg cagtcccacg
     7081 tattttcttt atcattccaa ttaacatggc attcagcgtg ggggcatttt gtgtaaaacg
     7141 ctttaacggt tccgttgtcg ttaaaaacgg cggtgtcttt gcctccgatg ttcacaattt
     7201 tgccctttcc tttcgggatg tcgttaatac tagccatatg gattttttta tcgttttccg
     7261 ctatccttat agtaatcttt ttggctcgga ttgccaaaaa agagactggt attaccccgg
     7321 caccagagca aagcctagtg ctgggccgag cgcggcattt tcaccacgtt agaaattatg
     7381 ggggctcata gtcccgattt tgtctctgct gtgcggagat gcaaaatcga gtcccgacgg
     7441 ctttaccatc gtggatcctc gatttcttta ttgtaaatta attgaaaatc gggagatgct
     7501 cgattttctt gctctggcaa agccagagag aaaatcggca taaagtggta ttacgcggca
     7561 ttcgtcccga tttatcaaat tttgaaatgt tttacgtcta tattttataa tcgaaacgga
     7621 ataaatcttt ttatattggg agttgtgaaa atatcgaaat acgtttaagg ctgcacaaca
     7681 agggattggt aaaatcaaca aaaagattat tccttgggaa gtcatatatt atgaagcgtt
     7741 ctatggttta gcagatgccc gatgcaaaga gaaacaaata aagtcttgga agaagagaag
     7801 cgcaattgaa cgaatgataa aacatttcaa aatttaggat aaatcgagga tcctcgatag
     7861 ttctggtggg ccc
//
LOCUS       DATDSU010000131         5463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_964535, whole genome shotgun sequence.
ACCESSION   DATDSU010000131 DATDSU010000000
VERSION     DATDSU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964535"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..84)
                     /locus_tag="VI981_04585"
     tRNA            complement(1..84)
                     /locus_tag="VI981_04585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Leu,seq:taa)
     gene            complement(155..229)
                     /locus_tag="VI981_04590"
     tRNA            complement(155..229)
                     /locus_tag="VI981_04590"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(195..197),aa:Cys,seq:gca)
     gene            complement(289..618)
                     /locus_tag="VI981_04595"
     CDS             complement(289..618)
                     /locus_tag="VI981_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEY4479600.1"
                     /translation="MNEHLKDEKLKEAIRVLASKFVEQESNGSSLITVTRIELMDHAK
                     RAFVMFTTLPDGKEAEALAFLERKRSEFRTYVREKSRIGRIPFFDFKIDLGEKNRQNV
                     DKLSNLQ"
     gene            complement(621..2138)
                     /gene="infB"
                     /locus_tag="VI981_04600"
     CDS             complement(621..2138)
                     /gene="infB"
                     /locus_tag="VI981_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY4479601.1"
                     /translation="MTKSDTKKLVERPPIIAVMGHIDHGKSTLLDYIRKTNTTLKEAG
                     GITQHVAAYEVKHQKDGTEKRITFLDTPGHEAFVKIRERGADVADIAILVVSAEEGVK
                     AQTLEALKCIKVSKTPYIVAINKIDSGKSDIERTKQNLAEHEIYIEGYGGDVPWVPCS
                     GKTGEGIDALLDMVLLVAEIEELKADPEARAEGVVIEAHLDTKKGTSATLIIKNGTLR
                     KGESIVAGSAFSPVRGMENYAGEQIEEASFSSPIKLIGFNVLPETGSIFHAFKNRKEA
                     EQAAQENVRSPKIKPTTKRSGNEVDDLVYIPVVIKADASGTLEAIFHEIKKIEHEKVK
                     VKVLDSGIGAISEGDIKTSLADEGTKVIGFNTKIDSLAKGAAERSGVEIAVFSIIYKL
                     TEWLEAIIKLKIPKITVHEITGRAKILKVFGKTKDKQIVGGRVETGLISSSGEIKLYR
                     RDQEIGRGRIRELQQQKKRTSEVKKDTEFGALIESKIEIAEGDRIECFTEVEKQS"
     gene            complement(2207..2680)
                     /locus_tag="VI981_04605"
     CDS             complement(2207..2680)
                     /locus_tag="VI981_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY4479602.1"
                     /translation="MVSAKLLPYVVYSLSGIVTALIIWIIFLEIRIKKLLGGKGKNLE
                     QSLLDFHKELGAYREFRGDIETYLIKVEKRMAKSLRGLGTVRFNALHGTGHGGVQSFA
                     TCYLNEKGDGIVVLALAVRDRTSFFTKSIKDFTSEFELTVEEKQAVAKARQSLAL"
     gene            complement(2737..3090)
                     /locus_tag="VI981_04610"
     CDS             complement(2737..3090)
                     /locus_tag="VI981_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479603.1"
                     /translation="MKNYLPSVSVQLGNIETGLIALKKQGWEAIVRPSGVIHLRRPGG
                     IHYCRCPLTALCASQLNIFIPFKMVHNAVQLLGLHHQTASIVVNSSDNQNGEETFDPN
                     FRKKILVIFGLLKVA"
     gene            3143..4435
                     /locus_tag="VI981_04615"
     CDS             3143..4435
                     /locus_tag="VI981_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY4479604.1"
                     /translation="MSTQKSRFDLFSDSRSNLRKRIYLDYASLTPVAPEVLKVIKKYS
                     SGEFANPASLYEEGVNAKKVLAEARKKTADFFHAHPDEIIFTGSGTEANNLAVRGLIA
                     KIRNCRPNVLFGTGVAKSVMPEDRSVLWRKIRKKRLHIITSVIEHVSVLEPIGVLEKE
                     GIDVTYVGVDSSGVVDIKELKKALRPETVLVSIQYVNNEIGTIQPISEIAKLLRHSSK
                     SIRYTLCAKPFFHTDASQATCYLPLYAEVLGADLVTIDAHKCYGPRGIGLLYKKRNVP
                     LLPLLFGGGQEYGFRPATENIPAIAGFAEALALIETRRARDVREITLLRDYFINKLLK
                     INPRIELNGDREKRLPNNVNISIKNIDNELFVLQLDAKGIAASTKSSCLRDEEESYVI
                     RALGKGQGAKTAVRFSMGRETTKAELEYTLQEIKHLLR"
     gene            4506..>5463
                     /locus_tag="VI981_04620"
     CDS             4506..>5463
                     /locus_tag="VI981_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY4479605.1"
                     /translation="MHKESKLVLLVGIVVLCFTSLMGLFLSNGGGIFRAGLNGKSLSA
                     QVAGSGSSIQARWNFEGQNCSGSTVNDADLLAPTLNLSVSNTTWKDTSFYEGSCTMKW
                     SSSGAYLSGGDVLDMGTGSRSVSFWFTTDTSDPTFTLVSKGAYASQAGRWYIAYCPSG
                     TPATAVCTSGQAGLTAGWQGSSLNVRTSPGITTGAGGASLANTSYHHVVAVWNRSGNL
                     SLWVDGGTNGAPQATVNISADSAYNSNTASPFLLGEMSSPTSPYTSPYSSEPAFNGSE
                     SRMDDIRVYDRVLSAAEITDLYSGGPQRYNGAQTGTLPAGTTS"
BASE COUNT         1391 a         1231 c         1235 g         1606 t
ORIGIN      
        1 tggtgcccgg ggagggaatc gaaccctcat ccttttggga cacgatttta agtcgtgcgc
       61 gtataccagt tccgccaccc gggcaatttg cacatcgcaa aacagtttgc aactcattcc
      121 cgacgaaaac aaataaaatt gttttctccg ggcatggagg ccacggcggg aatcgaaccc
      181 gcgcatagaa gttttgcaga cttccgaatt accacttttc tacgtggcct tgtgttaatt
      241 ttcaattatc aatttagcaa ttttccgctc tggattcttg aaaattggtc attgtaaatt
      301 cgataattta tctacatttt gcctgttttt ttctcccaaa tcaattttaa agtcgaaaaa
      361 cgggattctt ccgattctgg atttttctcg aacgtaggtc cgaaactcac ttcgcttacg
      421 ctcgaggaac gcgagtgcct ccgcttcctt tccgtcagga agagtagtaa acatcacaaa
      481 tgcgcgcttg gcgtgatcca taagttctat tcgtgtcact gtgataagag aagaaccgtt
      541 tgattcctgt tcaacgaatt ttgaagctaa aacacgaata gcctctttca gtttttcatc
      601 tttgagatgt tcattcatag ttaggattgt ttttccactt cagtgaaaca ctcgattcgg
      661 tcgccttcgg caatctcaat ctttgattcg attaaagcgc cgaattccgt atcttttttg
      721 acttcactcg ttcgtttttt ctgttgttgc agttcgcgaa ttctgcctcg tccgatttct
      781 tgatcacgcc gatataactt aatttctcca ctcgaagata tcaatcctgt ttccactctg
      841 ccgccgacga tctgtttatc ttttgttttc ccaaatacct tcagtatttt tgctcggccg
      901 gtgatttcgt gaactgtaat tttgggaatt ttcaatttta ttattgcttc gagccattca
      961 gtcaatttgt atatgattga gaaaacagcg atttcaactc cggagcgttc ggcggcgccc
     1021 ttcgcgaggc tgtcgatttt ggtattgaaa ccgatcactt tggtaccctc atccgccaga
     1081 gacgttttaa tgtctccctc tgaaattgcg ccgattcctg aatctagaac ttttactttt
     1141 accttttcat gctcgatttt tttaatttcg tggaagattg cctcaagcgt cccgcttgcg
     1201 tcggctttta tgaccacagg aatgtagacg aggtcgtcaa cttcattccc actcctcttg
     1261 gtggtaggtt ttatttttgg cgatcgcacg ttttcttgtg ccgcctgttc tgcttctttt
     1321 cgatttttga aagcgtggaa tatgcttccc gtttctggaa gaacgttgaa tccgataagt
     1381 ttgatagggc ttgaaaaact ggcttcttcg atttgttccc cggcgtaatt ttccattcct
     1441 cgaactgggg aaaatgcact acctgcaact attgattcgc cttttcggag tgtgccgttt
     1501 ttgataataa gcgtcgctga cgtgcctttt tttgtgtcaa gatgtgcttc gataacaact
     1561 ccttcggcgc gagcctcggg atcggctttc agttcttcaa tttcggccac aagaagcacc
     1621 atgtcaagca gtgcatctat tccttcgcct gtctttccag aacagggaac ccatggaacg
     1681 tccccgccgt atccctcgat gtatatctca tgttctgcaa gattttgttt cgtacgttct
     1741 atatcagatt ttccagagtc gatcttgttt atcgctacaa tatatggagt cttgctaact
     1801 ttgatacatt tcaaagcctc gagcgtttgg gctttaactc cttcttcggc cgagacgacg
     1861 agaatggcaa tatctgcaac gtcggcgcca cgctcacgaa tttttacaaa tgcctcatgt
     1921 cctggagtgt ctaggaaagt gattcttttt tccgtgccgt ctttttggtg tttaacttca
     1981 tacgccgcca catgttgggt aattccgccc gcttctttaa gtgtcgtatt tgtttttcgt
     2041 atatagtcga gaagtgttga ttttccatgg tcaatatgcc ccattacggc aataataggg
     2101 ggtcgttcca cgagtttttt tgtatcgctt ttcgtcatgg atgtatagcc cgaaatcaga
     2161 atatagcacg attgtgcgat tcatatccaa cgatatggtc tctctgctaa agagcaagac
     2221 tttgtctcgc tttcgcaaca gcttgttttt cttcgacggt taattcgaat tcagaagtga
     2281 aatctttgat tgatttagtg aaaaatgaag tgcgatcccg aacggcaagg gccaatacga
     2341 caattccgtc tcctttttca ttgaggtagc atgttgcaaa gctttggacg ccgccatgtc
     2401 ccgtaccgtg gagtgcattg aaacggacag tgccgagacc ccgaagagat tttgccatgc
     2461 gtttttcaac tttgattagg tatgtttcga tgtcgcctct aaattcgcga tacgcgccga
     2521 gctccttgtg aaaatctaaa agtgattgct caagattctt accttttcct ccgagcaatt
     2581 tttttattcg aatttcaaga aagataatcc aaattattaa ggcagttaca atacctgaaa
     2641 gggaatacac aacatatggc aatagttttg cggacaccat taggagaaaa ttatactcct
     2701 tgccgtgata aagaaaaatc ccgccggggc gggaggtcag gcgactttta gaaggccgaa
     2761 aatcacaagg atttttttgc gaaagttagg atcgaacgtc tcttctccgt tctggttgtc
     2821 gctcgaattc acaactatcg aggctgtctg atgatgcaga ccgagaagct gaactgcatt
     2881 gtggaccatt ttgaacggaa taaaaatgtt tagctggctg gcgcaaagtg cagtaagcgg
     2941 acaacgacaa tagtggatgc cgcccggacg cctcaaatga atgacgccgc tgggtcgtac
     3001 gattgcttcc cagccttgct tcttgagcgc aatcagacct gtttcgatgt tgccaagttg
     3061 gacagacaca ctggggaggt aatttttcat aaagtttgat tgttgcagtt ttcttcactc
     3121 ttgatatcat ccaaacacga ttatgtcaac acagaaatca aggttcgacc ttttttccga
     3181 ttcaaggtcg aaccttagaa aaagaattta tcttgattat gcttcgctga cgccggttgc
     3241 gccggaagta ttgaaggtga taaaaaaata ttcaagcggt gaatttgcca atccggcatc
     3301 gctctatgaa gaaggagtca atgcaaaaaa agttttagcg gaagcgcgaa aaaaaacagc
     3361 cgactttttt cacgcacatc cagacgaaat tatttttaca ggaagcggaa ctgaagcaaa
     3421 taacctcgca gtcaggggac taattgcgaa aattcgaaat tgccgcccga acgtgctgtt
     3481 cggtacgggt gtggcgaaat ccgtcatgcc tgaggacaga tccgtccttt ggcggaaaat
     3541 ccgaaaaaaa agattgcata taattacttc tgtaattgaa cacgtatcag tgcttgagcc
     3601 aattggagta cttgagaaag agggaattga cgtgacctat gtgggcgttg attcaagcgg
     3661 agtggtagat atcaaagaac tgaaaaaagc cttgaggccg gaaaccgttc tcgtctcaat
     3721 tcagtatgta aataatgaaa ttggaacaat ccagccaatt agtgaaatcg ccaaacttct
     3781 tcgccactct tccaaatcta tacgctatac gctatgcgct aaacccttct ttcatactga
     3841 tgcctcacag gcgacttgct acctgccact ttatgctgaa gtgcttgggg cggatctcgt
     3901 cacaattgac gcccacaaat gctatggacc tcgaggaatt ggtttgcttt ataaaaaaag
     3961 aaatgttccg cttctgcctc ttttatttgg cggagggcaa gaatatggat ttcgaccggc
     4021 cacagaaaat attccagcga ttgcaggttt tgccgaagca ctagcactaa ttgaaacacg
     4081 gcgagcgcgt gacgtgcgag agataacatt gctccgagat tattttataa ataaactact
     4141 aaaaataaat ccgcgaattg aactcaacgg ggaccgtgaa aaacgtctac ccaacaacgt
     4201 aaatatttca ataaaaaata tagataatga attgtttgtc ctgcaacttg atgcgaaagg
     4261 aatcgccgct tcgacaaaaa gttcgtgtct tcgggacgaa gaagaatcgt atgtaattcg
     4321 ggctttgggg aaagggcagg gcgctaaaac ggctgttcga ttctccatgg ggcgagagac
     4381 gactaaagca gagcttgaat acacactcca agagataaag cacctgctta ggtagcatca
     4441 atatacacag ctattttggc caaaagcgca tataattata gccaagtctg cattgcagat
     4501 tattcatgca taaggaatca aaacttgtat tgctagtcgg tatcgtggtt ctctgtttca
     4561 cttcgcttat gggtcttttc ctttctaacg gaggaggcat tttccgtgcg ggcctcaatg
     4621 gaaaatcact ttcggcgcaa gttgctggaa gcggctcgtc gattcaggcg cgctggaatt
     4681 ttgaagggca gaattgttct ggctcaacag ttaatgacgc cgatttgctc gctcccacgc
     4741 tgaacttaag cgtgtccaat acaacttgga aggatacatc attttatgaa ggttcttgta
     4801 ccatgaaatg gagtagttca ggagcgtatt tgagtggtgg cgatgtactc gatatgggaa
     4861 caggcagtcg ctcagtttct ttttggttta ctacagacac gtctgaccca acattcacgt
     4921 tagtttccaa aggtgcatat gcttctcaag cgggacgctg gtacatcgcg tactgtccct
     4981 caggaactcc agcaacggct gtatgtacgt cggggcaagc gggtctcacg gctggatggc
     5041 agggcagtag tcttaatgtt cgaacgtctc cgggtataac gacaggagct ggcggtgcgt
     5101 cgcttgccaa tacgtcttat caccatgttg tcgcggtgtg gaatcgaagc ggtaacctgt
     5161 ctctctgggt agatggcggt acaaacgggg ctcctcaagc tacggtgaat ataagcgcgg
     5221 acagcgccta caattcaaat accgcctcgc cttttttgct cggggaaatg tcgagtccga
     5281 cgtcaccata cacaagtcct tactcgagtg agcctgcatt caacgggtct gaaagccgca
     5341 tggatgacat acgtgtctat gacagagtac tctctgccgc tgaaattacc gacctttaca
     5401 gtggaggccc tcaacggtac aacggcgctc agacgggtac gcttcctgcc ggcacgacgt
     5461 caa
//
LOCUS       DATDSU010000132        18759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2147807, whole genome shotgun sequence.
ACCESSION   DATDSU010000132 DATDSU010000000
VERSION     DATDSU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 18759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18759
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147807"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..672)
                     /locus_tag="VI981_04625"
     CDS             complement(253..672)
                     /locus_tag="VI981_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479606.1"
                     /translation="MILPMRKILLDKVALLIVVVFLTHLVATFFYLYWRFWWFDLLMH
                     LLGGVWLGLFSAWFVFFSGYVSERVATRRNIVLASLLTVVVLGGGWEVFEYVLGVADS
                     AAQTYSADVSTDFLMDTLGALIASSYMIGFLSKKKGV"
     gene            complement(994..1284)
                     /locus_tag="VI981_04630"
     CDS             complement(994..1284)
                     /locus_tag="VI981_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479607.1"
                     /translation="MRAENFEQFREDSERSESEPRMTITPIDGGFKLEIESDEEGVTA
                     IYRAVAEQFLGAYGHVERRPDDSLDVKTSKEVKSEEIISGIKHIAEMVRKES"
     gene            complement(1288..1893)
                     /locus_tag="VI981_04635"
     CDS             complement(1288..1893)
                     /locus_tag="VI981_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY4479608.1"
                     /translation="MSHPKKERTLVVIKPDALQRSLAGEIVSRFENVGLKIVGLKMFV
                     PGPKLIEEHYTLDPEWRRITGEKTIKGYRDKGMEPPSTDPYTITAKILKNLVMYLTAG
                     PVIAMVLEGAHSVAITRKLVGSTEPLASDVGTIRGDFVIDSYKMSDDDNRAVRNLVHA
                     SGSPKEAADEIGHWFTKEEIIQYKNVNEKILYDVNLDGILE"
     gene            complement(2000..4753)
                     /locus_tag="VI981_04640"
     CDS             complement(2000..4753)
                     /locus_tag="VI981_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEY4479609.1"
                     /translation="MEKVELALPNQKFTSPEEEIAFLREQVIRREKELFHFKQNTPEV
                     AASDIINAYKITPAQSVLSEEYKMPRSEEKTIVLDLTPETHDQKMSELLEVMQNKGIK
                     NTLVIVESLKNPHIEDDFERFLVQYIKGGYPLKGFKEREPVFRALRVTLYEISLPNAR
                     PEKEKALKELLSAMEQFYAGMLSVAESPGSQPAYFSLELAVANHSEEFIFYAAVPDGK
                     RDLFEKHILSLYPDAKIAVKKDDYNIFNEQGISAGSIAELTENPIFPIKTYDLFDHDP
                     LQVVLNSFSKIDKEGEGAAIQIIFNPMGDYYVKRYNEALMSIKKGKKVKEAINIPESF
                     LEHLGELARTIASTSVSKKNEKEKQEPVDERALKLIETKLSSAVVLANVRIVVSSKTE
                     EDAAQIISDIESAFNQFENTNSNKIRFRRVKRGELASLLHYFTFRLFSRNTALPLSLR
                     ELSTVLHFPSSVTHAQPQLKIAKAKTAAAPVDLPREGILMGINNDRNVETKIFMNQED
                     RMRHFYAVGQTGTGKTSLLKNMILQDIDRGDGVCMIDPHGTDIQDVLAHIPPSRYDDV
                     IYFDPSYTARPMALNMLEYDTRYPEQKTFVVNEMLSIFDKLFDMKVAGGPMFEQYFRN
                     SVLLTIEDPESGSTLVDVSRVLANKSFRELKLSRCKNPIVVQFWKEVAEKAGGEASLA
                     NIVPYITSKFDNFLSNDIMRPIIAQERSSFNFREVMDTRKILLVNLSKGRLGDLNSHL
                     IGLILVGKILMAALSRVDSLSGDPAPFYLYIDEFQNITTTSIATILSEARKYRLSLHM
                     AHQFIAQLDEKIKDAVFGNVGSMACFRVGAEDAEFLAKQFEPVFTAQDLINLDNHNAY
                     VKLLVKGKTVQPFSLETIRPPQGNNDVADKLKELSYLKFGRPRAEVEAEVMQKYQKKS
                     ASI"
     gene            complement(4950..5177)
                     /locus_tag="VI981_04645"
     CDS             complement(4950..5177)
                     /locus_tag="VI981_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479610.1"
                     /translation="MDISETDDVDQRFLSAIHEVEEAIKIENTWILKEKIEAARKIKD
                     EMTDAGMIADADHQLREYASKLSDLEEQENA"
     gene            complement(5266..6033)
                     /gene="map"
                     /locus_tag="VI981_04650"
     CDS             complement(5266..6033)
                     /gene="map"
                     /locus_tag="VI981_04650"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY4479611.1"
                     /translation="MINIYSSQEIKILQQGGKRLAEIINAVCEKVASGVSTLELEILA
                     RTLIEKNGDKPSFLGYTPSGAKRPYPAATCISINEEIIHGIPNERPKILKEGDIVTID
                     IGLIHEKLCTDSARTVGVGALDQISRELLKATARALENGIKAARRGRHVGDIGYAIET
                     FVEKTPFSIAEDLCGHGVGKNVHEDPYVPNRGRKGDGPLLKEGMVLAIEPMLNAGTAK
                     VVLAEDGYTYRTADGKRSAHFEHTIAITTGEPLILTE"
     gene            complement(6030..6644)
                     /locus_tag="VI981_04655"
     CDS             complement(6030..6644)
                     /locus_tag="VI981_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY4479612.1"
                     /translation="MQPQTFIFFGRSGSGKGTQAILLKQHLEKKAGGRKVLYVETGAR
                     LREFIKKEYKTSQLTSDIMHAGGLLPEFIPIWIWTQYLIENFTGEEHLILDGLSRRVA
                     EAPVLDSALRFYGFSGKEKKIVISINASKEGCVKRLLGRGRPDDTREDIEERLSWYDT
                     NVSPVLDYFKKNSEYIFLEINGDQPIPKVHSEIVQKVFSSKVTS"
     gene            6972..7535
                     /locus_tag="VI981_04660"
     CDS             6972..7535
                     /locus_tag="VI981_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479613.1"
                     /translation="MKIRHLLGIFLASCLNMRGEETNVFHPNMSVEKLALLPNPTTNR
                     TPVDIAVLRTKLDDLLIHSLHLHGALTNLLTRAENYSTPLSTNEKSSASATLRLHDAA
                     RTVSLYHAHYQQMFGRNGLKADAKNVVENALLYTLRKMEWVSRRKESPRDDYFYSYFY
                     TLLRYREVRDGWLILNGTFTQVPTERN"
     gene            complement(8048..9322)
                     /gene="secY"
                     /locus_tag="VI981_04665"
     CDS             complement(8048..9322)
                     /gene="secY"
                     /locus_tag="VI981_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY4479614.1"
                     /translation="MTTNWRDKASLLLKDKVIVKKIAFVLIGFAIFRLFSVIPIPGVD
                     PLQLEKFFQDNQFFGLLNIFSGSGLATLSIVMLGVGPYITASIIMQLLTMMSSKLKAL
                     YHEEGEAGRQRFAQYSRRISVPLALLQGLGFLALLTRQGAINTLSPLDFAVNLMVITA
                     GAVLLMWLGELMTEYGVGNGTSLIIFGGIVSRVPAIVSQQYFVLTGQPELIPRYLGFL
                     AAAILIVWAVVVMTEAERPIPITYAKRVRGNKIYGGISTYLPLRINQAGVIPIIFALS
                     IILIPQMIFNFTKNIDAIAPIANKILAVLNNGWFYSVFYFLLVFVFTYFYTAVTFDPK
                     TISENLQKSGAFIPGVRPGGSTMEYLAKVVTRITLVGAIFLALIAVLPLAMQSITGNQ
                     ALAIGGTALLIVVSVVLDLVKKIGGQIAMREY"
     gene            complement(9434..9874)
                     /locus_tag="VI981_04670"
     CDS             complement(9434..9874)
                     /locus_tag="VI981_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HEY4479615.1"
                     /translation="MQFHTLKRVHKNKKRTIVGRGGKRGKTSGRGTKGQKARAGRKIR
                     PEMRDMIKRLPKLRGRGKNIFKSFAIPAKVIGLDLIEKNFSNGDTISPTSLREKNLVS
                     GLNGRSLRIKILGAENFSKKVTVVDCTVSANARLMVEKAGGKIM"
     gene            complement(9937..10575)
                     /locus_tag="VI981_04675"
     CDS             complement(9937..10575)
                     /locus_tag="VI981_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY4479616.1"
                     /translation="MNDIEIKKEKETTGAVATEPIKVVAAEPIKRVEYRERKKNPRRQ
                     GGRPERVKPEFDQKIISIRRVTRVAAGGRRFSFSVALVAGDRKGRVGVGTGKAGDTSI
                     AIDKALKNAKKNMIRVTTTPDFSIPHRVAAKYSSAIVQIFPARGKGLVAGSSVRNVLE
                     LAGVKDVNAKVLSGSKNRLNIARVAIKALSMLRIKKQEAQTPKHDLPAGRQE"
     gene            complement(10585..10935)
                     /gene="rplR"
                     /locus_tag="VI981_04680"
     CDS             complement(10585..10935)
                     /gene="rplR"
                     /locus_tag="VI981_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY4479617.1"
                     /translation="MSRVTNKTEKRIRRHKRIRAKIKGTAVIPRLAVFRSNKYIYAQL
                     VDDVSGTTIASVSSLAKKGEKKKITAKAVGSQIAERAKSAKIKKVVFDRSGFLYAGKI
                     KAVAEGAREGGLDF"
     gene            complement(10939..11478)
                     /gene="rplF"
                     /locus_tag="VI981_04685"
     CDS             complement(10939..11478)
                     /gene="rplF"
                     /locus_tag="VI981_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY4479618.1"
                     /translation="MSRIGKQQIAIPEKTTVTVNGTIVVVHGPLGELSRAIHPLIGVT
                     IDSGKVITRPVKETKDSNTLWGTSLSHIENMIVGVNKAYEKKLIIEGIGYKAEIKGAE
                     LVLALGFSHPVKVKIPAGLKVTSEKGVISISGIDIEAVGSFAAAVRGLKKPEPYKGKG
                     IRYEKEVIRRKEGKKTGTT"
     gene            complement(11579..11974)
                     /gene="rpsH"
                     /locus_tag="VI981_04690"
     CDS             complement(11579..11974)
                     /gene="rpsH"
                     /locus_tag="VI981_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY4479619.1"
                     /translation="MARDQVAELINKIHNAGAVGHAVVTFGYSELSHGIALALEKEGY
                     LKSSLKKGKKGGKMLEIEINYDEFGKSLVHGVKRLSHLSKRIYKKSRELTPSRGSYEH
                     VILSTPAGVLSDKDARAKHVGGEVLFKIW"
     gene            complement(12109..12294)
                     /locus_tag="VI981_04695"
     CDS             complement(12109..12294)
                     /locus_tag="VI981_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY4479620.1"
                     /translation="MAKKSVIARAKRKPKYKSRVVRRCFRCGRKRGYIRDFDLCRICF
                     REFANEGMIPGIKKSSW"
     gene            complement(12462..13013)
                     /gene="rplE"
                     /locus_tag="VI981_04700"
     CDS             complement(12462..13013)
                     /gene="rplE"
                     /locus_tag="VI981_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY4479621.1"
                     /translation="MTTKTNISPDPFEALKKKWHHSNIMQMPRITKIVLSAGIGSIKD
                     KKKIELVSDRLTRIAGQKVVTKGAKKSIASFKVRQGDPVGYEVTLRGKRMHGFLLKLL
                     NVTLPRTRDFRGLQESSVDEMGNLTIGIREHTIFPETVDEDLKDVFGLAVTLVSSARN
                     REEALDYFKAIGFPFGKSYKVEK"
     gene            complement(13018..13326)
                     /gene="rplX"
                     /locus_tag="VI981_04705"
     CDS             complement(13018..13326)
                     /gene="rplX"
                     /locus_tag="VI981_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY4479622.1"
                     /translation="MLIKKDDNIIVITGKDKGKTGKVTRAFPRQAMVLIQGFNKVKRH
                     QKARRQGKKGEIVEKSLPMHVSNVMLVDPKTGKRTRVGKKNIDGKMVRIARKSGIEIK
                     "
     gene            complement(13422..13793)
                     /gene="rplN"
                     /locus_tag="VI981_04710"
     CDS             complement(13422..13793)
                     /gene="rplN"
                     /locus_tag="VI981_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY4479623.1"
                     /translation="MIQPRSIVKIADNTGGKIGRIFKVLGSSKKRYAELGEVVVLSIQ
                     KAEPRKAVKKKDVLHAVVVRQRNPYRRKDGSYIRFDENAVVIIEKGKRDPIAGRVFGP
                     IPREIQEAGFQKIASLAPEIV"
     gene            complement(13815..14051)
                     /gene="rpsQ"
                     /locus_tag="VI981_04715"
     CDS             complement(13815..14051)
                     /gene="rpsQ"
                     /locus_tag="VI981_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY4479624.1"
                     /translation="MKKQINRKQFKGKVVSDKMKDTIVVEVNRFKKVPKYEKYIKVTK
                     RFMAHDPGNTKKIGDVVEIEETRPISKRKHFRVI"
     gene            complement(14056..14244)
                     /gene="rpmC"
                     /locus_tag="VI981_04720"
     CDS             complement(14056..14244)
                     /gene="rpmC"
                     /locus_tag="VI981_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY4479625.1"
                     /translation="MKELNKKNDKELEKELRARSESLREFRFGIAGSKLKNVREGRSI
                     RKEIARIKTELNTRKHNA"
     gene            complement(14351..14770)
                     /gene="rplP"
                     /locus_tag="VI981_04725"
     CDS             complement(14351..14770)
                     /gene="rplP"
                     /locus_tag="VI981_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY4479626.1"
                     /translation="MLLPKKIKHRKWHTMRHNPKKPHVETRGAELSFGSFGLKAMTAH
                     RIRSNQIESARKVMSRYVGKVGKIWIRIFPDMPFTAKSGEVPMGSGKGDPQGYQVQVW
                     PGRVLFEIDGIAETEAREALRKAGTKLPLKTKIVARI"
     gene            complement(14868..15536)
                     /gene="rpsC"
                     /locus_tag="VI981_04730"
     CDS             complement(14868..15536)
                     /gene="rpsC"
                     /locus_tag="VI981_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY4479627.1"
                     /translation="MSHVVHPYSHRLGIIRDWKSRWFGADKKKYRDFLKADLIIRENL
                     TEKLRGFYVSSIEMERSEKTLRVIIKTSRPGMIIGKSGDGAVKLRQHILTLIRKMKLL
                     MPPELKLDIEEIRSPESNAAIVAQMVADGLEKRLPFRRVIKQMIEKVMANRDVKGVKI
                     YLTGRLGGAEMARQEELKKGQIPLQTFRADIDFAREKAHMTYGDIGIKVWIYRGEIFK
                     KDKK"
     gene            complement(15547..15903)
                     /gene="rplV"
                     /locus_tag="VI981_04735"
     CDS             complement(15547..15903)
                     /gene="rplV"
                     /locus_tag="VI981_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY4479628.1"
                     /translation="MKTELKTYRQSPRKVRLVANLVKGRSVLEALSTLSFAQKEAGVA
                     IHKLLRSAIANAKHNHGIEKEALFVKDIQVNPGVVLKRIMPRARGSAAQILKRTSHIT
                     LFLDTKSPDNKLKAKS"
     gene            complement(16008..16349)
                     /gene="rpsS"
                     /locus_tag="VI981_04740"
     CDS             complement(16008..16349)
                     /gene="rpsS"
                     /locus_tag="VI981_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY4479629.1"
                     /translation="MSRSLKKGPYVSENLLKKISGKRPEQAGVVRTWERDSVIAPEMI
                     GFKFGVHNGKDHIEVLVAEEMVGHRLGEFALTRKFLRHGGKMQKELEMKKKEAEIAAA
                     AAAKAVTEEKK"
     gene            complement(16775..17614)
                     /gene="rplB"
                     /locus_tag="VI981_04745"
     CDS             complement(16775..17614)
                     /gene="rplB"
                     /locus_tag="VI981_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY4479630.1"
                     /translation="MKHYNPTSKSRRQMTTISYGKVLTSSTPHKSLTYGFKRKMGRNS
                     FGRITTRHKGGGHKRLFRDVDFMYDKKNIEARIDTVEYDPNRTAFIALVTYKDGEKRY
                     SVIPKGLTAGDTFIVNDKADLKVGNRMQLKNIPVGTFVYNVELKPGGGAKIGRSAGNY
                     VEVVAIEGGAAHIKMPSSEIRKVSENCFASIGEVSNEENRLVVIGKAGRSRWLGIRPT
                     VRGTAMNPVDHPYGGGEGRQGRGTRRAKTMWGKPSGKGQKTRRAKKYSNVLIVSRRKV
                     GKK"
     gene            complement(17911..18294)
                     /locus_tag="VI981_04750"
     CDS             complement(17911..18294)
                     /locus_tag="VI981_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY4479631.1"
                     /translation="MAFFSKTKSEKGAERKPAEVSKKEIMKAPVLAQSEAFEDARSVI
                     LRPRVTEKAALLNERHTYVFEVARNATKRTVARAIQMLYKIEPVAVRIVPIRSKTLFM
                     KGKRGSTQRGKKAYVELKATDKIEV"
     gene            complement(18297..>18759)
                     /gene="rplD"
                     /locus_tag="VI981_04755"
     CDS             complement(18297..>18759)
                     /gene="rplD"
                     /locus_tag="VI981_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY4479632.1"
                     /translation="GTGRARHGSIRSPLWVGGGVAHGPRNDKNFARKVNKHMKALALA
                     TIICKKMKDGEVLFLDTLKLEEAKTKLAKNVLAALSKIQGFEALTRKKQNAALVAIPS
                     KSTSTERGFRNIGTVSISELRNLNPLDVLRYKYLLISEPDQSVKYIAQKIS"
BASE COUNT         4802 a         4389 c         3792 g         5776 t
ORIGIN      
        1 gctgtcagac gtttttcgtg aaggcgcacc cgccatttgt agtataagcg tctcagtacc
       61 tcgactactt gattttattg agataggtag taaatacata atacttggat ttctaacggg
      121 attcatgggg tagtgaaaaa tggcatcgca ttaagatgcg acaaaccacc aaattataaa
      181 tcggccaata ttttataaat gataaccata ttttaaaagt gtatcgtcta atgccatttt
      241 ctactacccc attcacactc cttttttctt cgacagaaaa ccaatcatgt aagaagaagc
      301 gatgagcgct cccaacgtgt ccatgagaaa atctgttgaa acatcggcgg aatatgtctg
      361 cgcagcactg tcggcaacgc cgagtacata ttcaaaaact tcccatcctc cgccgagcac
      421 tacgaccgtc agaagcgatg caagaacgat atttcttctc gtcgcaactc tctcacttac
      481 atatccggaa aaaaacacaa accatgcgct gaaaagacca agccacacac ctcctaaaag
      541 gtgcatgaga agatcaaacc accagaatct ccagtataaa taaaagaagg tggctacaag
      601 atgggttaag aacacgacaa caatgagcag tgcaacttta tcgagcagta ttttccgcat
      661 gggtagtatc atagcagaag tgttaagaaa atatgtgtcg caagtttttt atgcacaata
      721 aataccccgg aggggcattc attgttgggt tacttgaagt atttacctag caaacgccag
      781 gtgggtgtcc gcaacctaat cggtcagaac ccctccccat ttttacaagg ttcgaccttg
      841 aatcgggaat aaggtcgaac cttaatccca aaatggggag ggactcatcg cgaccggcaa
      901 tgtaagactc cctaaaaatg ttttatagag ttaatacatc aaataaccct gcttatagta
      961 tacatcctct actgaaaggt ttacaagcaa tttttaactt tctttgcgaa ccatttcagc
     1021 aatatgcttg attccggata tgatttcctc actcttcact tccttgcttg tcttaacgtc
     1081 gagactgtcg tcaggtcgcc tctctacgtg gccataggcg cctaaaaact gctcggcaac
     1141 tgctctgtaa atcgccgtaa caccctcttc gtccgattct atctcaagtt taaaaccacc
     1201 gtcaatggga gtaatggtca ttcgtggttc cgactccgaa cgttcggagt cttctcgaaa
     1261 ttgctcaaag ttctccgctc tcatatatca ctctaaaata ccgtcgaggt taacgtcgta
     1321 gagaattttt tcgttcacat ttttatactg aatgatctcc tcctttgtga accagtgccc
     1381 aatttcatcc gcagcctctt tggggcttcc ggatgcgtgc actaaattac ggacggctcg
     1441 gttgtcatca tctgacattt tatatgaatc aatgacaaaa tcccctcgaa tcgttccgac
     1501 gtcagaagcg agcggttcag tgctgcccac gagcttcctc gtaattgcca ccgaatgagc
     1561 cccctcaaga accattgcta taacgggtcc ggcggtcaaa tacatcacca agttcttcaa
     1621 gattttggct gtaatggtgt atgggtcggt agacggcggt tccattcctt tgtcgcggta
     1681 gccttttatg gttttttctc ctgtgatgcg tctccattcg ggatcaagcg tatagtgctc
     1741 ttctattagt tttggcccag ggacaaacat cttaagccca actattttca agcccacatt
     1801 ttcaaacctt gaaacaattt caccggcaag tgaacgctga agcgcatcgg gcttgataac
     1861 gacaagtgtt ctttcttttt ttggatgtga catgacgctt acactaatac taatatacta
     1921 attgttacca atatacgaat ggatacgaat aaatctgatt agtattattt gcatattagt
     1981 actcatttgt atattggtat tatatactag cactcttttt ctgatatttc tgcattactt
     2041 cagcttcaac ctccgcccgc ggccgcccga atttaagata cgaaagttct ttgagcttat
     2101 cggcaacgtc attattgcct tggggcggcc tgatggtttc caggctaaac ggctgtactg
     2161 tctttccttt aacgagtaat ttcacatagg cattatgatt gtccaaatta atgaggtcct
     2221 gcgcagtgaa aacaggctca aactgcttgg ctaaaaattc cgcatcttca gcgcctaccc
     2281 tgaaacacgc catggaacca acgtttccaa agactgcgtc ttttattttc tcatcaagct
     2341 gggcgataaa ttgatgcgcc atgtgaagac ttaaacggta ttttctcgcc tcggataata
     2401 tggtggcaat tgacgtagta gttatattct ggaattcatc tatgtacaaa tagaagggcg
     2461 cgggatcccc cgaaagcgaa tcaacgcggg aaagtgcagc catcaaaatt ttacccacta
     2521 aaataagtcc tatgagatgc gagttcaaat caccaagccg ccctttggag aggttgacga
     2581 gaagaatttt tcgcgtgtcc atgacttccc taaaattaaa cgaggatcgc tcttgagcga
     2641 taatcggccg catgatgtcg ttcgacaaaa agttgtcgaa cttgcttgtg atgtacggaa
     2701 caatattggc aagcgatgct tcgccaccgg ctttttccgc cacttccttc caaaactgca
     2761 cgacaatcgg gtttttacat cgggagagct tcagttcacg gaaagattta ttggcaagga
     2821 cacgagagac atcgacgagc gtactgccgc tttccgggtc ctcaatcgtg agcaaaacag
     2881 agttccgaaa atattgctca aacatcggac cccctgctac tttcatatca aataatttat
     2941 cgaatatgct aagcatttcg ttcacgacga acgtcttctg ttccggatag cgcgtgtcat
     3001 actcgagcat gttgagagcc atgggccgcg cagtatagct tggatcaaag tagataacat
     3061 cgtcatatcg cgacggtggg atgtgggcaa gaacatcttg aatatccgtt ccgtgtgggt
     3121 caatcataca aacgccgtca ccgcggtcta tatcctgaag tatcatattt ttcaggagcg
     3181 acgttttacc tgtacccgtc tgaccaaccg cataaaagtg ccgcatacga tcttcctggt
     3241 tcataaaaat cttcgtctca acgtttcggt cattatttat tcccataaga attccctctc
     3301 gaggcaggtc aacgggtgcc gcggcggttt ttgcctttgc gattttaagt tgcggctgtg
     3361 catgagtcac tgaactcggg aaatgcaaaa ccgtggaaag ttcgcgcagg gagagcggca
     3421 gtgccgtatt tcgagagaac aatctaaaag tgaagtaatg gagtaaactg gcaagttctc
     3481 cccgctttac gcgacgaaaa cggattttat tgctattggt gttttcgaat tgattaaacg
     3541 cggactctat atcggagatg atttgagccg catcctcttc tgtctttgat gaaacaacga
     3601 tccgcacgtt cgcaagcacc actgccgaag aaagtttggt ctctataagc ttgagcgctc
     3661 gctcgtctac aggttcttgt ttttcctttt cgtttttctt tgaaacactg gtcgacgcaa
     3721 tagtacgagc taattcgccg agatgttcca agaaagattc tgggatatta atggcttctt
     3781 tgactttttt gcccttttta atgctcatga gcgcttcgtt atagcgcttc acgtaatagt
     3841 ctcccatggg attgaatatt atctgaattg cggctccttc accttctttg tcgatttttg
     3901 aaaaactgtt taagacaact tgcagagggt cgtgatcgaa caaatcgtac gtttttattg
     3961 gaaaaatagg attttcagtc agttcagcaa ttgaacccgc cgatattccc tgttcattga
     4021 aaatattgta atcgtctttc tttacggcta ttttcgcatc cgggtaaagc gaaagaatat
     4081 gcttttcaaa cagatcacgt tttccatccg gcacggccgc ataaaatata aattcttcac
     4141 tgtggttggc gaccgcaagt tcgagagaaa aatatgcagg ttgtgaacct ggactctcgg
     4201 caaccgagag catgccggcg taaaactgtt ccatggcgga aagcagttct ttcagcgcct
     4261 tttccttttc aggtctcgcg ttcggaagcg aaatttcata gagcgtcacc cgaagcgccc
     4321 gaaaaaccgg ctcgcgttct ttgaaacctt tgagcgggta ccctcctttt atatattgaa
     4381 caagaaaccg ctcaaaatcg tcttcaatgt gcggattttt aagcgattca acgatcacaa
     4441 gagtgttctt aatcccctta ttctgcatga cctccagtaa ttccgacatt ttctggtcat
     4501 gtgtttctgg agtcaagtcg aggacaatag ttttttcttc cgaccgcggc attttatatt
     4561 cttctgacag gactgactgg gctggggtta ttttgtaggc attgataata tcactcgccg
     4621 ctacttcggg cgtattctgc ttgaaatgaa ataattcttt ctcgcgccga ataacctgtt
     4681 cccttaagaa cgcgatttcc tcttcaggag aagtaaattt ttgattcgga agagcaagct
     4741 caactttttc catcccgtta gagacctgcc tgccatcgcc tgacacactg atggaaggat
     4801 gtgtaattaa ttagtaatat cattttacaa tttgcttatg tatcgatcta atcaacgaat
     4861 agtgcgaatg aaacgaatgg aagacaggta tgttttctga ttcgcatcat tcgtatgatt
     4921 agtatattcg tatcgataca gttttgtcct caagcgtttt cttgttcctc aagatcagaa
     4981 agttttgacg catattctct caactggtga tctgcgtccg caatcatacc agcatccgtc
     5041 atctcgtctt ttatcttgcg tgcagcctca atcttctctt tgagaatcca ggtattttct
     5101 attttgatcg cctcttcaac ttcgtggata gcgcttaaaa agcgctggtc cacatcatct
     5161 gtttcggata tatccataca tgtatatttc ttaaacgatt aatttccgct ggggaaattt
     5221 caagcttttc cagcatgtaa agtataacgc gcatcaaaat gaaagtcatt cggtcagtat
     5281 gagaggctca cctgtcgtta ttgcaatcgt gtgttcaaaa tgcgcacttc gtttgccgtc
     5341 ggccgttcga tacgtatatc cgtcttcggc gagaaccact ttcgccgtgc ccgcgtttag
     5401 catgggttca atcgctaaaa ccattccttc cttaagaagc ggaccgtcgc ctttcctgcc
     5461 ccgatttggc acatatggat cctcgtgtac atttttaccg acaccgtgac cgcataaatc
     5521 ttccgcaata gagaaagggg tcttttccac gaaagtttct atggcatacc caatatctcc
     5581 gacgtgtctg ccgcggcgcg cagctttgat tccgttttct agtgccctcg cagtcgcctt
     5641 cagcaattct ctagaaattt gatctagagc gccgactccg accgtgcggg cagaatctgt
     5701 acataatttt tcatgtatga gtcctatgtc aatagtgacg atatcacctt ctttcagtat
     5761 tttggggcgc tcgtttggaa ttccgtggat gatctcttca ttgatcgata tgcaagtggc
     5821 cgcaggatag ggccgctttg cgcccgaggg cgtataaccc aaaaaagagg gcttgtcgcc
     5881 atttttttca ataagtgttc gagctaaaat ttcaagttcg agcgtactga ctccagatgc
     5941 cactttttca catactgcgt tgatgatttc cgccagtctc tttccgcctt gctgaaggat
     6001 ttttatctcc tggcttgaat atatattaat cacgaagtta ctttggagga aaaaactttc
     6061 tgcactattt ccgaatgtac ttttggaata ggttgatccc cgttaatttc gagaaatata
     6121 tattcagaat tttttttaaa ataatcgagt acaggcgaaa catttgtgtc gtaccatgag
     6181 agacgctctt cgatatcctc tctagtatca tcaggacgac cccgcccaag caatcgcttc
     6241 acacatccct cctttgaggc gtttatactt ataacgatct ttttttcttt acccgaaaaa
     6301 ccataaaaac gcagggctga atcaagaacc ggcgcttcag ctacccttct cgataaccca
     6361 tcgagaatta aatgctcctc gcctgtaaaa ttttcaatga gatattgcgt ccaaatccag
     6421 attggaataa attcaggaag caatcctcca gcatgcatga tatcactcgt aagttggctc
     6481 gttttgtact ccttcttaat aaattcgcga agccgagcgc cagtttcaac gtagagcact
     6541 ttgcggccgc cagctttttt ctcaagatgt tgcttaagta atatggcttg agtgcctttg
     6601 ccagaacccg accggccgaa gaaaatgaaa gtttgaggtt gcatttagtg agtagctata
     6661 tgggttaaat aaaattggcc tcgccctatc cccgcagcaa gctctgcctc gcaaagcctc
     6721 ggcggggtat taggcgaggc atcagggcaa actttgttgc agaagtgtcg gctccgccga
     6781 cttctctttg tatttgcgga ttcaaccccg cagcaagctg tcggcgtatt ctccgcaact
     6841 gcaataaaaa tatgggttta gtatagcaat ttatgtaaag aatttgcaaa agatacaatt
     6901 gtttcgtgaa tttcatgcaa ttggctttcc gtacaaggag gtgtagagta ttcattgaaa
     6961 ggaattgctc catgaaaata agacacctac tcggcatctt tttagccagt tgcctcaaca
     7021 tgcggggaga ggaaacgaac gtgtttcatc caaacatgtc cgttgagaaa ttggcactgc
     7081 ttccaaatcc aactacaaac agaacgcctg tagacatcgc cgtccttcgg acgaaacttg
     7141 atgatttgtt gattcacagt ttacatctcc acggggcgct caccaacttg cttacgcgtg
     7201 ctgaaaacta ctcgacgcct ctatcaacaa atgagaaatc gtccgccagt gccacgcttc
     7261 gccttcacga cgccgcacga acagtaagcc tctatcacgc tcactatcag cagatgttcg
     7321 gacgaaacgg cctcaaggca gacgcgaaaa atgtcgtcga aaatgctttg ctctacacct
     7381 taagaaagat ggagtgggta tcgcggagaa aggaaagtcc aagggacgat tatttctact
     7441 cttacttcta cactcttctt cgctataggg aggtacgtga cggctggctc atcctgaacg
     7501 gcaccttcac acaagtacca acggagagaa actaatcggc ctactttgcc gccgcattta
     7561 ccagcagcgg ctttttttct tgcaaatatt agtcgaaaat gagtgaagtt taacttcctt
     7621 catttaaata atacattttg attctcgtct agaagacgca attttcgtca gggtgacgct
     7681 tcgcctgtca gagtaaggcc agtcccctct cttctgttat tttccaccgg agcaagtaca
     7741 tttcttttta aatgccttgg ggggcgcatc ggcgccagac agagaaaatc tttacccaaa
     7801 gattttcgtg cattttaaaa ggaatgtgcg agccgagcac cagaatggtg cttcggcgag
     7861 cgtctaatcc cagtatcaaa ctttgcgtta tattttggag tcaagacgct acacccaaaa
     7921 gggttggcct attcggccac gcgtctcgca tttgcaatat taagttcgca tacaaaatgt
     7981 gaatatcaaa ggcaaagtta ttacgggatt gtagcaaaac atagtcgcct agggctcctt
     8041 gtttttctca atattctctc atcgcgattt gtccaccgat ttttttaaca agatcaagta
     8101 ccacagaaac aacgataaga agtgccgtac cgcctattgc tagagcctgg ttacctgtaa
     8161 tgctctgcat agcaagaggc aaaaccgcga tcagggctaa aaagatcgca cccacaagag
     8221 taatccttgt cacaaccttt gcgagatatt ccattgtaga accgccgggc cggacgccgg
     8281 gaatgaacgc gccgcttttt tgcaagtttt cagatatagt ctttgggtca aacgtaacag
     8341 ctgtatagaa gtacgtaaat acaaaaacca gcagaaaata aaagaccgaa taaaaccagc
     8401 cgttattcaa gacagcgaga atcttgttgg caatcggagc aattgcgtca atattttttg
     8461 tgaagttgaa aatcatctgc ggaatcagaa taatagaaag cgcaaagatg atcgggataa
     8521 cacccgcctg attgatgcgc aacggaagat aggttgaaat accgccgtat attttgtttc
     8581 cccttacgcg cttggcgtag gtgatgggaa ttggtcgctc cgcttcagtc atgactacaa
     8641 ccgcccagac gatgagaatg gcggcggcga gaaatcccaa gtatcgtgga ataagctcgg
     8701 gctgaccggt cagcacgaaa tattgctgcg agacaatcgc gggcacgcgt gatacgatgc
     8761 cgccgaaaat tatgagcgac gtgccattgc cgactccata ttcagtcatg agttcgccaa
     8821 gccacataag caatacggca cctgcggtga ttaccataag gtttactgca aagtcgaggg
     8881 gtgaaagggt attaattgca ccctggcgag tgagcagcgc taaaaaacca agtccctgca
     8941 agagtgcgag cggtacagag attcttcgag agtattgggc gaaacgctga cggccggctt
     9001 ctccttcttc atgatagagc gctttcaact ttgaagacat catggtgaga agctgcataa
     9061 tgatcgacgc tgtgatgtaa ggtccgacac caagcatcac aatcgagagt gttgccagtc
     9121 cgcttccgga aaatatattg aggaggccaa aaaattggtt atcctggaaa aatttctcaa
     9181 gttgcaaagg atccactccg ggaatcggaa tgactgaaaa aagtctgaat attgcgaaac
     9241 caattaaaac aaaagcaatc tttttaacga tcactttatc ctttagcaaa agggatgctt
     9301 tatctctcca gtttgtggtc ataaaatact acgtagtatc gattctaatc aacgaatagt
     9361 acgaatgaaa cgaatggaac gcaagtgttt tttattcgta tcattcgcat aatttgtata
     9421 ttcgcatcga tacttacatt atctttccac ctgctttctc gaccataaga cgagcatttg
     9481 cgctaactgt acaatcgact acagttactt tcttactgaa gttttcggct ccaagaatct
     9541 ttatcctcaa gcttcggccg tttaatccgg ataccaagtt tttctcacgg agcgacgttg
     9601 gacttattgt atcaccattt gaaaagttct tttcaatgag atcaagccca atgacttttg
     9661 ccggtatcgc aaaacttttg aaaatatttt ttccgcggcc gcgcaacttg gggagacgct
     9721 ttatcatatc gcgcatctcc ggacggattt tgcggccggc acgcgccttt tggccctttg
     9781 tgcctcttcc cgatgtcttg ccgcgtttgc ctccacggcc aacgattgtc ctctttttat
     9841 ttttgtgcac tctttttagt gtgtgaaatt gcatggtgtt ttatgtgaat tcaaaatctt
     9901 aaattcgtac tcgtatcaaa ttttaaacat ttgaatttat tcctgcctac cggcgggcag
     9961 gtcgtgcttc ggggtttgcg cttcctgctt ctttatacgc aacatgctaa gcgccttgat
    10021 cgcgacgcgg gcaatattca gacgattttt gctgcccgag agtactttag cgtttacatc
    10081 ttttactcca gctaattcaa gtacgttacg gacagaacta cctgcaacta gacccttgcc
    10141 ccgcgccgga aaaatctgaa cgatagcact tgaatattta gccgcgaccc ggtgtggaat
    10201 agaaaaatca ggagttgtcg tgacccgaat catgtttttc tttgcatttt ttagggcctt
    10261 atctatggcg attgatgtat ctccggcctt ccccgtaccg acgccaacac ggccctttcg
    10321 atcacctgca acaagcgcta ctgaaaaact aaaacgtctg ccgccagccg ccacacgggt
    10381 tacacgtcgt atgcttataa tcttctgatc aaattcaggc ttaacacgct ccggtcttcc
    10441 gccttgccga cgaggatttt ttttgcgttc tctgtattcg actcttttaa tcggctcggc
    10501 cgctactact tttatgggtt ctgtggcaac tgcccccgtt gtttcctttt ctttttttat
    10561 ttctatgtcg ttcataattg taatttagaa atcgagtcct ccctcgcgcg caccttctgc
    10621 gacagctttg atttttcccg cgtaaagaaa tcctgatcga tcaaaaacga cttttttaat
    10681 ttttgcagat ttggcgcgtt ctgcaatctg tgatccgact gcttttgcgg ttattttttt
    10741 cttttctcct ttcttcgcaa gtgacgacac agaggcgatc gtagtacctg atacgtcatc
    10801 aacgagttgc gcatagatat atttgttcga tcgaaatacg gcaagtctcg gaattacagc
    10861 cgttcctttt atctttgcgc gaatcctttt atggcgccga atcctttttt cagttttatt
    10921 ggtaaccctg ctcatgtttt atgtagtgcc cgttttcttg ccttcttttc gtcgaataac
    10981 ttctttttca tatcgaatcc ctttaccttt gtatggttcg ggttttttta gcccccgaac
    11041 ggcggcggca aaacttccca ccgcttcaat atcgatgcct gaaattgaaa tgaccccttt
    11101 ttccgatgtc accttaagtc ctgccggaat tttgactttt acaggatgcg aaaatccaag
    11161 tgcaagcaca agttcggctc cttttatttc cgctttgtat ccgataccct cgatgatgag
    11221 ctttttttcg tatgccttgt tgactcccac aatcatgttt tcgatgtgcg aaaggctcgt
    11281 cccccagagt gtattagaat cctttgtttc tttgacgggc cttgtgataa cttttcctga
    11341 atctatagtc acaccgatta ggggatgaat agcccgagaa agttcgccga gcggcccatg
    11401 tactacgaca atagtaccgt tgacggtcac ggttgttttt tcaggtattg cgatttgttg
    11461 tttgccaatt ctgctcatgt aattctaata tactaattca taccaattat acgaatggaa
    11521 acaaatgcat ttttctttat tagtttcatt ggtatacatt cgtatattgg tattattgct
    11581 accaaatttt aaaaaggact tctccaccga cgtgtttggc tcgtgcatct ttatcggaga
    11641 gaacgcccgc cggagttgac aaaattacat gctcgtacga gcccctggaa ggcgtaagtt
    11701 ctctgctttt cttataaata cgtttggaaa gatgtgagag gcgctttact ccatgcacga
    11761 gagatttgcc gaattcatca taattgattt caatctcaag catcttgcca cctttcttgc
    11821 ctttttttag tgagctttta agatatcctt ctttttcaag cgccagagct attccgtgtg
    11881 ataactccga gtatccaaaa gtaactacag cgtgaccaac ggcacccgcg ttatgtatct
    11941 tatttatgag ttctgctact tgatctcgcg ccatatgatt gatatttaag gagtgaaacg
    12001 actataaaaa tcatcaccca gctgtagttt tgatttatcg aaatttcaat cgaccaaaac
    12061 tacggctgga taataaaaat ctaattcgct ttgctcataa catttttatt accatgaaga
    12121 tttcttaatg cccggtatca tgccttcgtt tgcaaattct cggaaacaga tgcgacagag
    12181 atcaaaatcc ctgatatagc cgcgttttcg gccacacctg aaacagcgcc gaacaactcg
    12241 acttttatat tttggttttc ttttcgctcg cgcgataact gatttcttgg ccatttttac
    12301 aacaaaaaaa tctcctccat ccccagtttt gactgattga aatcaatgca tcaaaactgg
    12361 ggatggacaa gaaaaatata ctcgtaaata ctcgctattt ttcaagaggt ttaaacatac
    12421 tagcaagagg gctagagcct gtcaaagtgc cggcttttta gctacttttc caccttgtag
    12481 cttttcccaa atgggaaacc aattgcttta aagtagtcga gtgcttcttc cctgttccga
    12541 gcacttgata caagtgtcac ggcgagcccg aacacatcct tcaaatcttc gtctacagtt
    12601 tcagggaaaa tagtgtgttc tcgaatcccg atcgtcaaat tgcccatttc atcgaccgaa
    12661 ctttcttgta agccgcgaaa atctctggtt ctcggcagag tcacattaag caattttaag
    12721 agaaaaccat gcattcgttt gccgcgcagc gtcacttcat agccgacggg atcaccttgt
    12781 cgaaccttaa atgacgcaat tgatttcttt gctcccttcg taacaacttt ctgtcctgca
    12841 atacgagtaa gtcggtcact gacgagttca atcttcttct tatccttgat cgacccaata
    12901 cctgcggaca ggacgatttt tgtaattcga ggcatctgca tgatgttcga atggtgccac
    12961 ttctttttaa gggcttcaaa tggatccggt gagatgtttg ttttagtcgt catataatta
    13021 ttttatttcg ataccactct ttcgagcaat tcgaaccatt ttaccgtcaa tatttttctt
    13081 cccgactctt gttctcttac cggtttttgg atcaaccagc ataacattgg aaacgtgcat
    13141 cggcagactt ttttcgacaa tttcgccttt tttcccttgg cgacgcgcct tttggtgccg
    13201 cttaaccttg ttaaagccct gaataagcac catggcctgg cgcggaaagg ctcgcgtaac
    13261 tttgccggtc ttacctttgt ccttgcctgt tataactatt atattgtcgt cttttttgat
    13321 taacataata caaatatact aattgttacc aatatgcgaa tggatacgaa taaatctgac
    13381 tagtattatt tgtatattag tattcattcg tatattggta tttaaactat ctccggagct
    13441 aatgaagcaa ttttctgaaa accagcctct tggatctctc ttggaattgg accaaagacg
    13501 cgtccagcaa tcggatctcg ttttcctttt tcaataataa cgacggcatt ttcgtcaaat
    13561 ctaatatatg acccgtcttt tctgcggtat ggattgcgct gccgcacaac tactgcatgg
    13621 aggacatctt ttttcttcac tgcttttcgg ggttccgctt tctgtattga aagaaccact
    13681 acttcgccga gctctgcata tcttttttta gaactgccga gcaccttgaa aatgcggcca
    13741 atttttccgc ctgtgttatc tgctatttta actatggatc gaggctgaat cataaatact
    13801 tcgtattatc gatactaaat cactctaaag tgttttctct tcgaaatggg ccttgtttct
    13861 tcaatttcta ccacatctcc aatttttttg gtatttccgg gatcgtgcgc cataaatcgc
    13921 tttgtaacct taatgtattt ttcgtatttc ggcacttttt tgaaacgatt aacttcgaca
    13981 acaatcgtgt ccttcatttt atctgacaca actttgccct taaattgttt tctgttgatt
    14041 tgctttttca tattattagg cgttatgttt gcgggtattg agttcggtct tgatccgcgc
    14101 gatctctttt ctaatggatc gcccttcgcg gacattttta agcttgctac cagctatacc
    14161 gaaacgaaat tctcttaagc tttcggatcg agcacgtagt tccttttcca attccttgtc
    14221 gtttttttta ttcagttcct tcataaattc actgtatcga ttctaatcaa cgaatactac
    14281 gaatgaaacg aatggaatgc aagtattttt attcgtatca ttagcataat tcgtatattc
    14341 gcgtcgatac ttatattcta gccactatct tagtcttcag cggcaattta gtccccgctt
    14401 ttcttaaagc ctctcttgct tctgtttcag cgattccgtc gatctcgaaa agcactctgc
    14461 cgggccagac ttggacttga taaccttgcg gatcgccctt tccggaaccc attggtacct
    14521 caccactctt tgctgtgaaa ggcatgtcgg gaaatattcg aatccaaatt tttccgactt
    14581 tgccgacata tctagacata acttttcgcg ctgactctat ctgattagag cgaattcgat
    14641 gcgcagtcat agccttgagg ccaaaagagc cgaaggaaag ctctgctcct cgcgtttcga
    14701 cgtgaggctt tttaggatta tgcctcatcg tgtgccattt tctatgtttt atctttttgg
    14761 gcagtaacat aaaaatacta atatactaat tcataccaat tatacgaatg aatgcaaatg
    14821 agattattct gttattagta tcattggtat acattcgtat attggtgtta tttcttatct
    14881 ttcttaaata tttctcctct ataaatccag accttaattc caatatcgcc gtaggtcata
    14941 tgggcctttt cacgggcaaa atcaatgtct gcgcgaaacg tttgaagcgg aatctgacct
    15001 ttctttaact cttcctggcg agccatttca gctccgccca atcgtccggt caaataaatc
    15061 ttgacccctt tcacgtcacg attggccatg actttttcta tcatttgttt tatgactcgt
    15121 ctaaacggca ggcgtttttc aagaccatca gcgaccattt gagctacgat ggctgcattt
    15181 gattcgggag agcgaatttc ctcaatatca agtttcaatt caggaggcat gagcaatttc
    15241 atttttcgga taagcgtaag gatatgctgt ctcaatttga cagcgccatc tccgctctta
    15301 ccgataatca tgccggggcg cgaagttttg attatgacac gaagcgtttt ttcgcttcgt
    15361 tccatttcta tagaactgac atagaaacct ctaagcttct cggtgagatt ttcgcggata
    15421 atgagatcag ctttgagaaa atctcggtac ttctttttgt cagcgccaaa ccagcgagat
    15481 ttccagtccc ggataatgcc taatctatgt gaatatggat ggacgacgtg agacataaaa
    15541 tagcttttag cttttagctt ttagcttatt atccggtgac ttcgtatcta aaaacaaagt
    15601 gatgtggctg gttcttttta aaatctgtgc agcactgcct cgggctctcg gcatgattct
    15661 tttcagcact actccgggat taacctgtat gtccttcaca aaaagagcct ctttttctat
    15721 tccatggtta tgtttcgcat tcgcgatcgc cgacctaagc aacttgtgga ttgccactcc
    15781 cgcttctttt tgagcaaaag aaagggtcga tagtgcttca agtacagatc gacccttaac
    15841 aagattcgca acaaggcgaa cttttcgagg tgattgtcga tatgttttga gttcggtttt
    15901 catcatgtaa ttctaatcta ctaattcata ccaattatac gaatagacgc aaatgagatt
    15961 attcttttat tagtatcatt ggtatacatt cgtatattgg cattaggcta ttttttctct
    16021 tccgtcacag ccttcgccgc cgccgcagct gcgatttcag cttccttctt cttcatttcg
    16081 agttcttttt gcatcttgcc tccatgtctt agaaacttac gcgttaaagc aaattctccc
    16141 aaacggtgac cgaccatttc ctcggctact agcacttcga tatgatcctt gccgttatgc
    16201 acgccgaact tgaaaccgat catttcgggg gctatgacac tgtcgcgttc ccacgttcga
    16261 acaaccccgg cttgttcggg tcgttttccg gaaatctttt tcagaagatt ttccgacaca
    16321 tatggtcctt ttttcagcga tcgggacatg acaaaaatta aaaagcccct ttggagcgtt
    16381 ttgacatact aaacaatagg ctatctgttg tcaaacgagc attttttaaa gaattcgggc
    16441 ttctggaaac gatgtgccaa cattcaggac atttagatat tcaaatgttt ttttcatcag
    16501 taccagacaa ctacgcggtc atttccgccg ggggcaagag cacaacccgc aatatttaca
    16561 acatacccgg cgccgccacc ctaacctgca cctcttactg ctgcgcttac taagacattt
    16621 aaaaaggaat gggcgagcgc ccctttatgc ttcctttttt caactatgcg aggatagttg
    16681 cacgaaagtg ccttgcgcag accgacgggt cgagcagagc aatccaccag gaggcagata
    16741 tgcgtgcact ttcgtgcaac tgtccgagcg ctctttactt cttgccaacc tttcggcgac
    16801 tgacaataag cacattcgaa tattttttag ctcgtcttgt tttttgtcct tttccagatg
    16861 gtttccccca catcgtttta gctctacgag ttccgcgtcc ctgtctccct tctcctccac
    16921 cgtatgggtg atctaccgga ttcattgccg ttccacgcac tgttgggcga attccgagcc
    16981 agcgtgatcg gccagctttt ccgataacta cgagcctgtt ctcctcgtta gaaacttcac
    17041 caatcgaagc aaagcaattt tctgatacct tacgaatctc cgaggagggc atttttatgt
    17101 gtgctgcccc cccttctatc gccacgactt caacgtaatt gcctgccgat ctgccgattt
    17161 tcgcgccgcc tcctggtttc aattcaacgt tatagacgaa tgtacctacg ggtatgtttt
    17221 tcaactgcat tctgtttcca actttgaggt ctgctttatc gttaacaata aacgtgtcgc
    17281 cggcagtgag ccctttggga atcacgctgt agcgtttctc tccatccttg tatgtcacga
    17341 gcgcaataaa agctgttcgg tttggatcat actctaccgt atcgatacgc gcttcaatat
    17401 ttttcttgtc atacataaaa tcaacatccc ggaaaagtcg tttgtggccg cctcccttgt
    17461 gtcgcgtggt aatccggcca aagctgtttc ttcccatttt cctcttgaat ccgtaggtga
    17521 gcgatttatg cggagtcgac gaagttaaaa cctttccata tgaaatggtt gtcatctgcc
    17581 ggcgtgattt acttgtagga ttgtagtgtt tcatcccgtt agaaatagga aacgctcaac
    17641 tttgattgct gaggttcgat gttaataaca tatgaataat attcgccaaa caatatttgt
    17701 ccagcgtttc cgtagacaga cttggatatc gcttcttcat ttaccgacag caattttggg
    17761 aaatatgttt gtaaatacat aacaataaga tttctaacgg gatttcataa aaaagttcta
    17821 atatactaat tgttacaaat atacgaacgg atacgaataa atctgattag tattatttgt
    17881 atattagtac tcattcgtat attggcatta ttaaacttct atcttatctg tcgccttaag
    17941 ttcgacatag gcctttttac ctcgctgtgt cgagcccctt ttgcccttca taaaaagcgt
    18001 cttgcttctt atgggcacga tccgaacggc aacaggttcg attttataga gcatttgaat
    18061 ggcgcgggcc acggttcgct ttgtagcatt tcttgccact tcaaaaacat aagtatggcg
    18121 ttcgtttaaa agcgctgcct tctcggtaac acgcgggcgc aaaataactg accgagcatc
    18181 ttcaaatgct tcgctttggg caagtacagg agctttcatt atttcttttt tagaaacttc
    18241 ggcaggtttg cgctccgcac ccttctctga ttttgttttg ctaaaaaaag ccatggttat
    18301 gaaatttttt gagctatata tttgacagac tgatctggct cgctaatgag taaatattta
    18361 tatcgaagta catctaaagg atttaaattt cgcagttcac tgatgcttac ggtcccaatg
    18421 tttcggaaac ctctttctgt cgaagtcgac ttcgaaggaa tcgcgaccag cgcggcattt
    18481 tgtttctttc gtgtaagtgc ttcaaatccc tgaatcttcg agagagcagc gagcacattt
    18541 ttcgctaatt ttgtttttgc ctcttcgagt tttagagtat ccaaaaacaa aacttctccg
    18601 tccttcattt ttttgcaaat aatagtcgcg agggcaagtg ctttcatgtg tttatttacc
    18661 tttcgtgcaa aattcttatc gttgcgaggt ccatgtgcaa ctccacctcc tacccaaagc
    18721 ggcgaacgaa tggatccatg gcgagcacgt cccgttccc
//
LOCUS       DATDSU010000133         6810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1476337, whole genome shotgun sequence.
ACCESSION   DATDSU010000133 DATDSU010000000
VERSION     DATDSU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476337"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..712)
                     /locus_tag="VI981_04760"
     CDS             complement(386..712)
                     /locus_tag="VI981_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY4479633.1"
                     /translation="MKLGIILQSNKPEHVWNTFRLGITALKAGNEAEVFLMSEGSELD
                     TIPDSKDFDISAKIAEFKNLKGKIYACGSCLKIRGKEESNVCPVSTMSDLLKMVEDSD
                     KVLVFG"
     gene            complement(825..3182)
                     /locus_tag="VI981_04765"
     CDS             complement(825..3182)
                     /locus_tag="VI981_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4479634.1"
                     /translation="MTIQKIQHIKKYKSFRDYSWQPFLNNENFHEEVNILYGENGCGK
                     TSVCNILKSVSQNKNFSRYFPEEVGLLIDDTEYKYENKLWTSSISNSSILFFDREFVD
                     KNVHTGRDRGTQQGEQEQESGKLIIEFDGEAIKLREVRDALAKTKDEHGKTFDDFKRS
                     NTKTLSFELTDEEEVFFRKHKSKSKSDLKKLKETLEEQKSELGGSIKSDQKLLQKATE
                     IQSLEELEEPDASINFSQEAGYQKVFDFDLKEQVKIKAEEELIRKIKEHKDFFEAGFE
                     IRKIASHECPFCQAKDKEENITEIIRVYNQIYDDSYRKQKAAFDSYKRSLTDELKEIQ
                     ETLRNLNLDNIFIRIKRFSEQYSIKELYSLEEEEGFRKKRPTQKIDELEKKISGLEKP
                     SKENISALYGEVSKEVKSITELLAKLIVLVEKKNELIKLFKKEHTSKKLVERMGKASE
                     KLGGIKSELAFIDSSKIDNQKLKLEKDKELNKLTSVFEKSKEEHKSAREKYEEYCSRE
                     AFEKTLKRIQSYFKNFNFNFQLKLDTSNRHTGSTKEFPFAFRVVDPDGNERDLKEGLS
                     EGEMQVLSLCFFFAWLDIQNDKDQKVLVFDDPITSLDDSNLSSLVDLIADEKKKFSQI
                     FILTHHRTFFKFLRKRFDKHSAEYNIVRNKGHLGGSFICKSMEGRFIQRLQEFETHLV
                     NLGKNPAGFDIELQIVEYGQYLRYETEYFIKCRLLHWNESNEFGKVIEGIKENKKVSN
                     TDLDTIKKIYSFCNWTTSHVDVGDDHGLSQLKEKITDFIGVSDKY"
     gene            complement(3186..3977)
                     /locus_tag="VI981_04770"
     CDS             complement(3186..3977)
                     /locus_tag="VI981_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEY4479635.1"
                     /translation="MELESFKLSALEALSKIIGDNRTGSEISELFRKAGFPEISHDTG
                     TKWRFVYATLEQMQKQKYGTHNILKLIQQFCDPQEYFSAPERHTEVCKEVNQILSFYG
                     LGIGDDGKIRKLGERTTSLTKAIPENIRIFDERKFHGKISQHARKLFIEGNYFHAVFE
                     CCKVYDKDIQAKSQMSEYGAKLMELALALSGTLKLNAQQTRSERSEQEGVMHLSIGLM
                     RAIRNPQAHEPALDWPIDREDALDLLSFLNLLFRKLDKTTYWKNP"
     gene            complement(3982..6663)
                     /locus_tag="VI981_04775"
     CDS             complement(3982..6663)
                     /locus_tag="VI981_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY4479636.1"
                     /translation="MALHKNFPTSPYDILDPAIRWFPADENLREQGYEKLLPPLVAEL
                     RKKVKEWRLSGYEGASETSKALLKWWFLTDHPTELASGEIFLFKYYFAQREALETIIW
                     LYEVAKARDKYDLIRFDSSNAISAGMFDESWTRYVIKMATGSGKTKVLSLILAWSYFH
                     KLYEKDSALARNFLVITPNIIVLDRIRADFDGLKIFFEDPVLPDNGYHDKNWRDDFQM
                     TLHIQDEVGVVKKSGNIFLTNIHRIYEGATESASFGDPDTVDYFLGKKPVGKTNESKI
                     DLGDIVRNVDELVILNDEAHHIHDPRLAWFKAIEDIHNRLKMKGGQLSLQVDVTATPK
                     RNNGSIFVQTVSDYPLVEAIHQNVVKHPVLPDSASRAKLQEHKSASYTERYKDYLHLG
                     FLEWRKVYDQHIKVNKKAVLFIMTDDTKNCDDVAEYLENTYPELKDSVLVIHTKDNGE
                     ISESVSGKSEEELKILRQAANEVDRLDNPYKAIVSVLMLKEGWDVRNVTTIVGLRAYA
                     AQSNILPEQTLGRGLRRMYRDPELTELVSVVGTDAFMDFVESIKSEGVELEHKKMGEG
                     TEPKAPLIVEIDRENLKKDIEKLDIEIPVLTPRIYREYKNLSGLDVAKFGHKKIGLKE
                     FSEEEKREIIFHDIASGEITHKTELDSNFIPNYQSVVGYFTQNIMKALRLVSGYDILY
                     GKVKEFIQDELFTKHIELDDLNSLRNLSEIEASRTIFETFQKEINALTVLDKGEAEIR
                     DYIKISKSRPFVVKDQGYLVPKKSVFNKIVADSHFELEFASFLEDCDDIISYAKNYFA
                     VHFKIDYRNADGSISDYYPDFIVKVSPKEYYVVETKGREDLDDVEKIKRLEQWCGDVN
                     ASQKNSTFKMLYIKQEGWDKQQQRPRNFQEVIRGWSK"
BASE COUNT         1616 a         1570 c         1365 g         2259 t
ORIGIN      
        1 ggcaataatc gtgagtgttt aggcggactt tccgctttcc ctgctagtca ttcgggcaat
       61 gagtgaatga ctacctgaat gactaggtaa accgcccccg tttttggtaa gattggtact
      121 agcgtgcttt aataaacact ccaagattaa agtagtagaa atttttccaa ttttttcttt
      181 ggcggtagcc aaagaaaacc attgattcac ggcaattccg cacgcttcgc gtgcttttag
      241 ggtgaggttc gaaaaatacg aagccgaaga tttttggagg gaggattttt tggaggggag
      301 gtcgaagacg aataccaaca cttgccccaa ccctcccgtg cgcgtgttga aagacttttg
      361 ccgacttgga gatcggcaaa ctcccttatc caaacaccag caccttatcc gaatcctcaa
      421 ccattttgag caggtcagac attgtggaga cagggcagac gttgctttct tctttgccgc
      481 gtatttttag acaagagccg caggcgtaga tttttccttt caggtttttg aattccgcta
      541 tcttcgcgga aatatcaaag tccttactgt cgggaatggt atccagttcc gacccctcgc
      601 tcattaaaaa gacctctgcc tcgttcccgg ctttgagcgc agtgattccg aggcggaacg
      661 tattccacac gtgttctggt ttgttggatt gtaagatgat gccgagcttc ataaagccat
      721 tataccaact tgcctattga aactcctaac acttgcccca accctcccgt gcgcgtgttg
      781 aaagactttt gccgacttgg agatcggcaa actcccttat cgaatcagta tttatcggat
      841 acgccgataa aatcggttat tttttcttta agttgggaca acccgtggtc gtctccaaca
      901 tcaacgtgtg aggtagtcca attacaaaac gagtaaatct tctttattgt atccaagtct
      961 gtattagata ctttcttatt ttcctttatt ccttcaataa cttttccaaa ctcattcgat
     1021 tcattccagt ggagcaagcg gcacttgata aaatattccg tttcgtagcg tagatattgt
     1081 ccatattcaa cgatctgaag ctcaatgtcg aatccggcag gatttttacc aagattcaca
     1141 aggtgtgtct caaattcctg aagcctttgt ataaatctgc cttccatgct tttacaaata
     1201 aaactccccc ctaagtgtcc tttatttctg acaatgttgt attcggcgga atgtttatcg
     1261 aatcgctttc ggagaaattt aaagaaagtt cggtgatgtg tgagaataaa aatctgtgaa
     1321 aactttttct tctcatcagc aatcaaatcc acaagactac taagattact atcgtcgaga
     1381 ctggtaattg ggtcatcgaa gaccaagact ttttgatctt tatcgttttg aatatcgagc
     1441 caagcgaaaa agaagcagag cgacaagact tgcatttcac cctcggaaag accctccttt
     1501 aaatcccttt cgttgccgtc tgggtcaact accctgaagg caaacggaaa ttcttttgtt
     1561 gagccagtgt gtctgtttga tgtgtcgagt ttaagttgga agttaaaatt aaagttcttg
     1621 aaataagatt gtatcctttt taaggtcttt tcaaatgcct ctcttgaaca atattcctca
     1681 tatttctctc ttgccgattt atgctcttct tttgactttt caaaaactga cgttaatttg
     1741 tttagctcct tatctttctc aagcttgagt ttttgattat caattttact gctatcaata
     1801 aaagccaact cggacttgat acctcccaac ttctcagaag ccttgcccat tctctccaca
     1861 agttttttgc ttgtgtgttc ttttttgaat aatttaatca actcattctt tttttccacc
     1921 agaacaatga gtttagcgag aagttctgta atacttttca cctctttact tacttcgcca
     1981 tacaacgcgg agatattttc cttgcttggt ttttccagtc cagatatttt cttttccaac
     2041 tcatcaatct tctgtgttgg acgttttttc ctgaacccct cctcttcttc tagtgaataa
     2101 agttccttta ttgaatactg ttccgagaat ctttttattc taataaaaat attatcaaga
     2161 ttcagatttc taagcgtttc ttgaatctcc tttaattcat cagtgaggct gcgtttgtaa
     2221 ctatcaaacg ccgctttctg ttttctgtag gaatcgtcat aaatctggtt gtaaactcga
     2281 attatctccg tgatgttttc ttctttatcc tttgcttggc agaaaggaca ctcatgggaa
     2341 gcgatttttc ttatctcaaa accagcctcg aagaaatctt tatgctcttt tatctttcta
     2401 attagttcct cttctgcctt tatctttact tgctctttca aatcaaagtc gaataccttt
     2461 tggtatccgg cttcttgaga gaagtttatg cttgcgtccg gttcttcaag ttcttcgagc
     2521 gattgaatct ctgtagcttt ttgcaaaagt ttttgatcgc tcttaatact gcccccaagc
     2581 tccgattttt gttcctccaa tgtctcctta agttttttga ggtcgctctt gcttttcgat
     2641 ttatgttttc taaagaaaac ttcttcttcg tccgtaagtt cgaaggaaag agttttggta
     2701 ttgcttctct taaaatcatc gaaggtctta ccgtgttcgt cttttgtttt tgcaagagcg
     2761 tctctaacct cacggagctt tattgcctcg ccgtcgaact caattatcaa tttgcctgat
     2821 tcttgttctt gttcgccctg ttgcgtgccg cgatcacggc ctgtgtgaac atttttatcc
     2881 acaaactctc ggtcaaaaaa taagatcgag gagtttgaga tactgctcgt ccataatttg
     2941 ttctcgtatt tgtattcggt atcatcaatt aacaatccaa cttcttcagg aaagtatctg
     3001 ctgaagttct tattttggga tacgcttttt aagatattgc ataccgaggt tttaccgcag
     3061 ccattttctc cgtagaggat attgacctct tcgtggaagt tttcgttatt taaaaagggt
     3121 tgccaagagt aatctcggaa tgatttatat ttcttgatat gttggatttt ctgaatcgtc
     3181 atattctatg ggtttttcca ataggtcgtc ttgtctagtt tacgaaataa gaggtttaag
     3241 aatgacaaga gatcgagagc gtcttcgcga tcaatcggcc aatctagggc gggttcgtga
     3301 gcctgtggat tgcgaatagc tctcatcagg ccgattgata ggtgcatgac accttcttgt
     3361 tcgcttcgtt cagacctcgt ttgttgtgcg ttgagtttca gagtgcctga cagggcaagg
     3421 gctaattcca ttaactttgc accatactcg ctcatttgag atttcgcctg tatgtcctta
     3481 tcgtaaacct tacaacattc aaaaactgcg tgaaaataat ttccttctat aaataatttt
     3541 cgggcgtgtt gactaatctt gccgtggaac tttcgctcgt caaatattcg tatgttttcc
     3601 ggtatggcct ttgtgagtga ggtcgttctt tctcctagtt ttctaatttt cccgtcgtct
     3661 ccaattccta atccatagaa actaagtatc tgatttactt ccttgcagac ttccgtatgc
     3721 cgttcgggcg cagagaaata ttcctgcgga tcgcaaaact gctgtatgag ttttaggata
     3781 ttgtgcgtcc catacttctg tttttgcatt tgctcaagtg tcgcgtaaac aaaacgccac
     3841 ttagtcccag tgtcgtgcga tatttcgggg aagcccgctt tgcgaaaaag ctcggagatt
     3901 tccgagccag tacgattatc tcctataatc tttgacaatg cttcaagggc agataatttg
     3961 aaggattcca attccatagt gttatttact ccaaccgcgt atcacttctt ggaagtttct
     4021 cggcctttgc tgttgtttat cccaaccttc ctgctttata taaagcattt tgaaggtaga
     4081 gtttttctga ctggcgttca cgtctccgca ccattgctca agacgcttta ttttttcgac
     4141 atcatcaagg tcttcccgcc ccttcgtctc aacgacgtag tattcctttg gggagacttt
     4201 aacaataaag tcagggtagt agtcggaaat actgccgtcg gcgttccggt aatcaatctt
     4261 gaagtggact gcgaaataat ttttcgcgta tgaaattatg tcgtcgcagt cctctaagaa
     4321 actagcgaac tcaagctcga agtggctatc ggccacaatc ttattgaata cgctcttttt
     4381 cgggacaagg tatccttgat cctttacgac gaacggacgg ctcttgctga tttttatata
     4441 atcgcgtatc tctgcctctc ctttatcaag gacagtcagc gcgtttatct ccttttggaa
     4501 cgtctcgaag atagtccgcg aagcctcaat ctccgacaga ttgcggagcg aattgagatc
     4561 atcaagctct atgtgcttgg tgaatagctc gtcttgtata aactctttta ccttcccgta
     4621 gagaatatcg tagccactca ccaaacggag tgccttcata atattttgcg tgaaatatcc
     4681 gactacgctt tggtagttcg gaatgaagtt gctgtccaac tccgttttgt gcgttatctc
     4741 tccgctcgct atgtcgtgga aaataatctc ccgcttttct tcttcggaaa actctttcag
     4801 cccaattttc ttatggccga atttcgccac atcaagtccc gaaaggtttt tatattctcg
     4861 gtagattcgt ggtgttagga caggaatctc tatgtccaat ttctcaatat ccttcttgag
     4921 attctcccga tcaatttcta cgataagcgg agcttttggc tctgttcctt cgcccatttt
     4981 cttatgctca agttcaacgc cttcgctctt aatggattct acaaaatcca taaaggcgtc
     5041 cgtgccgacg acgcttacta gctccgttag ttcagggtcg cgatacatac ggcgtagtcc
     5101 gcgaccaagc gtctgttcgg gcaaaatgtt gctttgggcg gcgtaggcac gcaagccaac
     5161 aatcgtcgtt acatttcgta catcccaacc ttctttgagc ataagcaccg agacaatcgc
     5221 cttgtaggga ttgtccaagc gatctacttc attcgcggct tgccggagga ttttcaattc
     5281 ctcctcactc ttacccgata cactttccga tatttctccg ttgtctttcg tatgaataac
     5341 caacacagaa tctttcagtt ctgggtaggt attctcaaga tattccgcga catcatcgca
     5401 gtttttggtg tcgtcggtca ttatgaacag tactgccttt ttatttacct tgatgtgctg
     5461 gtcataaacc ttccgccatt ctaaaaagcc gaggtgtaaa taatctttgt atcgctccgt
     5521 gtagctcgca cttttatgct cctgtaattt tgccctactt gctgaatcgg gcaagacggg
     5581 gtgcttcact acgttttggt gaatcgcctc tacgagcgga tagtcgctta cagtctgcac
     5641 gaagatagag ccgttgtttc gtttcggcgt cgccgtaaca tcaacttgaa ggcttaattg
     5701 tccgcctttc attttcagcc gattgtgaat atcctcaatc gccttgaacc acgcgaggcg
     5761 cgggtcgtgt atgtggtgtg cctcgtcgtt gagtatcact aactcgtcca cgttgcggac
     5821 aatatcgccg aggtcaatct tcgattcgtt cgtcttaccg actggcttct tgcctaagaa
     5881 ataatctacc gtgtcgggat cgccgaagct cgccgattcc gttgctcctt cataaatgcg
     5941 gtgaatattt gtcaggaaaa tatttccgct tttctttact acccccactt cgtcttgaat
     6001 gtggagtgtc atttgaaaat cgtccctcca attcttgtcg tggtatccat tgtcgggcaa
     6061 tacggggtct tcaaaaaaga ttttcagtcc gtcgaagtcc gcacggatac ggtcaagaac
     6121 aatgatgttc ggagtgataa cgaggaagtt tcgagcgaga gcggaatctt tttcgtagag
     6181 tttatgaaaa tagctccacg ccaaaatcaa actcaagact tttgtcttgc ctgaacccgt
     6241 cgccattttt attacatacc gcgtccaact ctcgtcgaac attccggcgg atattgcgtt
     6301 agagctatca aaacgtatga gatcgtattt gtctcttgcc ttcgcaactt cgtagagcca
     6361 aatgatagtt tccaacgctt cgcgttgagc gaagtaatac ttgaaaagaa aaatctcccc
     6421 acttgcaagc tccgtcgggt gatcggtaag aaaccaccac ttgagtaagg ccttgctagt
     6481 ctcgcttgct ccttcatagc cggacaatcg ccattctttt actttcttgc gaagctccgc
     6541 gactaaaggc gggagcagtt tctcgtagcc ttgttcgcgt agattctcgt cagcaggaaa
     6601 ccaccggatt gccggatcga gaatgtcgta tggcgatgtc gggaagtttt tgtggagagc
     6661 catatattta tatttttatc tccacaacct tcattgtgtc attgccgaat atatcaacga
     6721 ctttcacggc aacctttcgc ttgccgggac ttacctccac cggcggtgta gtcagttgca
     6781 agtccctttt ctttttcgtg cggaagtcct
//
LOCUS       DATDSU010000134         6004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2460888, whole genome shotgun sequence.
ACCESSION   DATDSU010000134 DATDSU010000000
VERSION     DATDSU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460888"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VI981_04780"
     CDS             <1..293
                     /locus_tag="VI981_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479637.1"
                     /translation="KAWPWYFGQTQKNALIGACYVEKLLVFAYFIRLANPVNGGTRSV
                     VAASNGVNRTHLKHRPIFDEQSTKNGASNTLTMPFDRTTLRLLEKTFVGEAR"
     regulatory      263..383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01689"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin-variant (AdoCbl-variant)
                     riboswitch is found in the 5'-untranslated regions of
                     vitamin B12 (Cobalamin) related genes in bacteria; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01689"
     gene            447..2276
                     /locus_tag="VI981_04785"
     CDS             447..2276
                     /locus_tag="VI981_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY4479638.1"
                     /translation="MILHTWKRTASGVLASAFGLLLFIPLLFVRGNTDSALSYLETKS
                     QNAWSIMALTVNGRTIDPISLKAIDSTKAIDYEAPIMALVAAGKDPASYASLDYVAKL
                     KSFFDGTQLGDAALLNDDIFGILALKAAGMSNTESIITGLKNFIVSHQNADGGFSFLT
                     TGTSDTNTTAAAIMALRAAGNSVSDLTVEKAVAYLRQSQNDDGGFPYDPKSSWGTESD
                     GASDAWVIMALTAVNIDPSSFTKAGKSVVLHLMSLQAEQGYYKYQSASTEDSFSAVTT
                     AYAVLALTGKTLPAAAFAKQAYPHVSYRIAGSSEDLCVGETEAPNAFELVKIVSVSCG
                     FDYHVTEYSFGKYLDRIGTDSANGNKGWMYRINFTEPSVGAEAYVLKNDDAVLWYFDD
                     FNSKMARISLNPSHVSVGTPISATVEYYDAGEWKKLANATIHAKNYTAATDATGSATL
                     SLGEGHYNVFATAQGFLRTEQDTVIVGAKSESSISLSVDLGGTLSVPPPPQSDTISFT
                     LSGPGGNSDVGFGKISRGTNEKRSITVQNNGANAIYMESIITGDPVFTAYMRVDGLPW
                     REYSSEIEAKKSKDLELGLTVPTDYNGTGVKTGQLTVWATRSN"
     gene            2286..2828
                     /locus_tag="VI981_04790"
     CDS             2286..2828
                     /locus_tag="VI981_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479639.1"
                     /translation="MSKKTFYLVSVTLLLFISASHVFAAGLSVSPSKIEVFLSEGESA
                     SIRIKIGNPTKDVEVFEIYPDALEDVISVSPKSFTLESKEERIVLITVSALEPKTYET
                     DLSVVGRPLGSRSFQAGSGAKIPIKIIVDKKAFSLVRYRKIVKNPFTLIFLAVAIALG
                     SYKLGRVRRGRGTPQNASHP"
     gene            complement(2825..4408)
                     /gene="lysS"
                     /locus_tag="VI981_04795"
     CDS             complement(2825..4408)
                     /gene="lysS"
                     /locus_tag="VI981_04795"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY4479640.1"
                     /translation="MYWADQVAEEAKGRYKDKIKAGKPVVIRDEKTASGRIHVGSLRG
                     AAIHGVIGEALGEKNIANEFLWEINDFDVMDGLPGYLDEKTFRPHMGKLLCNVPSPDG
                     KAKNFAEYFSKEFTEVVEEIGLKPKYYRSSEVYKSGKYNECIRKILDNAATIRKIYKE
                     VSGADRDPSWLPLSVVCENCGKVGTTKATDWNGETVAYSCGKYVEWAVGCGHTGRVSP
                     LNGKAKLPWKVEWPSKFVIFDVDIEGGGKDHSTKGGPRDIAEAISRQVFKREPPINVP
                     YEWIYVGGKKMSSSKGTGSSAREMGDLLPPKILRFLLTHREPGKIVDFDPEGDTVPIL
                     FDTYDRFAKNYFSGGTDDFARAFYYSHPKLARADLKETALPRFSVVSFLAQMPHLDPV
                     KEISRMEERKLANIDIEELNERIECAKRWLKLYAPPDFKFDIQGIMPADARNLSEIQK
                     KALKTLLEFIESKQKLDGQEIHSRLHEMKVELSIEPRDLFEAIYISILGKPSGPKAGW
                     FLSVLDRTFLIERLREASL"
     gene            complement(4499..4966)
                     /gene="greA"
                     /locus_tag="VI981_04800"
     CDS             complement(4499..4966)
                     /gene="greA"
                     /locus_tag="VI981_04800"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4479641.1"
                     /translation="MPDQKEYLSKEKHDELAKELDHLRKVRRKEVAEDLEYAKSLGDL
                     SENAEYHEARELQANIEDRIQKLENILQNAEIMQLHHTEAVGIGSTVVVSRDTDGKEH
                     KFEIVGSEEADVSSGKLSIHSPLGEAMLGKRKSESFSFKSPRGVMKYKVVNIE"
     gene            5081..5698
                     /locus_tag="VI981_04805"
     CDS             5081..5698
                     /locus_tag="VI981_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479642.1"
                     /translation="MKYQTTLRRIHNLPWENLDTPKLEDLMVISGITAREYTNSLRIA
                     RTLLPEHKGLRLLTREEWRTSSFHFGSYSRKGDRADFLRHFVNKSGLLKTVPHLVGSA
                     CVTYDFKTGALPDDVRLMSVVSRERDLTRMMTRLLSSPPECWQGEALQAFRHYLARRI
                     RLRETGEGHLDPTSSIRVTNSVLKFYKLRLELYQSLFADHNTHSP"
BASE COUNT         1631 a         1489 c         1298 g         1586 t
ORIGIN      
        1 ctaaggcctg gccttggtat tttgggcaaa cacaaaaaaa cgctctaata ggagcctgtt
       61 acgtcgaaaa gttattggtt tttgcctact ttatccgctt agcaaacccc gttaacggcg
      121 gaacgcgatc cgtggtcgcg gcttctaacg gggtaaatcg gactcatttg aaacaccgac
      181 caatttttga cgaacaatcc acaaaaaacg gggcctctaa tacgttgaca atgcctttcg
      241 acagaactac actacgacta cttgaaaaaa catttgttgg ggaagccagg tgaaattcct
      301 gcacagtacc gctacggtta aagtccgaat accatttttg ttttttgtgt tcaagtcctc
      361 gagtaaggat tgtgaatatt attcggattc gtctccgcat aatgtcctac ccactcgggt
      421 aggacattat tattttttac attcgtatga ttttacatac atggaagcgc acagcgagcg
      481 gagtactcgc gagtgcattc ggtttattac tttttattcc gcttcttttt gtccgaggaa
      541 acaccgacag cgctctctca taccttgaaa cgaagtctca aaacgcatgg agtatcatgg
      601 ctcttacagt aaatggaaga acaattgacc ctatatctct gaaggcaatt gacagtacaa
      661 aagcaataga ctatgaagcc ccgattatgg cactcgttgc agcgggaaaa gatcctgcct
      721 catatgcgtc tctcgactac gtagcaaagc tcaaaagctt ctttgatggc acacaacttg
      781 gcgatgcggc gcttttaaat gacgacattt ttggcatact cgcactcaaa gccgccggaa
      841 tgagtaacac agaatcaata atcacaggcc taaaaaattt tattgtctcg catcagaacg
      901 ccgacggagg tttttctttt ctgacaactg gcacatcaga cacaaacacg acagccgcgg
      961 ccattatggc gctccgagca gcaggaaatt ctgtatctga tctaactgtc gagaaagccg
     1021 ttgcttacct ccggcaatct caaaacgacg atggaggatt tccatacgat ccgaaaagct
     1081 catggggcac cgaatcggac ggagcatcgg acgcgtgggt gatcatggct ctcactgcgg
     1141 tcaatatcga cccttcaagt tttaccaagg ccgggaaaag tgtcgttttg caccttatgt
     1201 ctcttcaggc cgagcaaggc tactacaaat atcaaagcgc gagtacggaa gattcatttt
     1261 ccgcagtgac cactgcgtac gctgtactgg ctcttaccgg taagacactt cccgcggcag
     1321 cattcgccaa gcaagcatat ccgcacgtat cgtatcgaat agccggatcg agcgaggatc
     1381 tttgtgtggg tgagacagag gctccaaacg ctttcgaact cgtcaaaatt gtctcggttt
     1441 catgcggctt cgactatcac gttacggaat attctttcgg aaaatatctt gaccgcatcg
     1501 gcaccgattc tgcaaacgga aacaaaggat ggatgtatag aatcaacttc acggagccat
     1561 cagtaggcgc ggaagcctac gtcctcaaaa atgatgacgc agtactatgg tactttgatg
     1621 actttaactc aaaaatggcg cgaatttcac ttaacccatc gcatgtttcc gtgggtacgc
     1681 cgattagcgc tactgttgaa tactatgatg ccggcgaatg gaaaaaactc gccaacgcga
     1741 caatccatgc aaaaaattac actgccgcca cagatgcaac aggcagtgct acgctctccc
     1801 tcggagaagg acattacaat gttttcgcaa cagcacaggg atttctacga accgagcaag
     1861 atacggtaat cgtcggcgca aagagcgagt cgagcatttc gctttcggtt gatcttggcg
     1921 gcactttatc agttccccca ccgccgcaaa gcgatacaat ttccttcaca ttgtcaggcc
     1981 ccggcggaaa cagcgatgtt ggatttggca aaattagccg tggcacaaac gagaagcggt
     2041 ccatcacagt gcaaaataac ggcgctaacg cgatctatat ggagtccatc atcacaggag
     2101 atccagtatt taccgcatac atgagagttg acggcttacc atggagagaa tattcaagtg
     2161 aaattgaagc aaagaaatca aaagatctcg agcttggtct caccgtaccg acggattaca
     2221 acggtacagg tgttaagaca ggacagctca ccgtttgggc gacccgcagc aattagtcgt
     2281 ccattatgtc aaaaaaaaca ttctatttag tcagcgtgac tctgcttctc tttatttctg
     2341 catcgcatgt ctttgcggcc gggttaagtg tttcgccgtc aaagattgaa gtattcctta
     2401 gcgaaggcga gtctgcaagt attcgcatca agatcggcaa tcctaccaag gatgtcgagg
     2461 tgtttgaaat ttatcccgac gcgcttgaag acgtaatttc agtaagtcct aaaagcttta
     2521 cgctcgaatc aaaagaggaa cgcatcgttt tgattacagt cagcgcgctc gaacctaaaa
     2581 catacgaaac agacctctcc gtcgtcggcc ggccgcttgg atcacgatcg tttcaagcgg
     2641 gatccggcgc aaaaattccg atcaaaatta ttgtcgataa aaaagcattc tcgcttgtga
     2701 gatatcgaaa aattgtgaaa aatccgttta ctctcatttt cctcgctgtt gcaatagcgc
     2761 tcggttcgta caaactcgga agagtgagga gaggcagagg cacacctcaa aacgcttcgc
     2821 acccttaaag agacgcctca cgaagtctct cgataagaaa cgtccgatca agcaccgaga
     2881 gaaaccagcc agcttttgga ccgcttggct tgccgagaat agatatatag attgcctcga
     2941 acaagtcccg aggttcaatt gaaagttcaa ctttcatttc gtggaggcgg ctatgtattt
     3001 cttgcccgtc caatttttgt ttcgactcta tgaattcgag cagggtcttt agtgcttttt
     3061 tttgtatttc ggataaattt cgtgcgtcag ctggcattat cccttggatg tcaaacttga
     3121 aatcaggcgg cgcatataat ttaagccatc gtttggcaca ttcaatgcgt tcattcaatt
     3181 cctcaatatc tatgttcgca agttttcttt cttccattcg gcttatttct tttacggggt
     3241 cgaggtgcgg catttgagcc aaaaaagaca cgacagaaaa tcgcggaagc gcagtttcct
     3301 ttagatctgc tcttgccaat ttagggtgag aataatagaa agcgcgggca aaatcatcag
     3361 ttccgcccga aaaatagttc ttggcaaaac gatcataggt atcaaaaaga attggcaccg
     3421 tatcgccttc gggatcaaaa tcgacaatct tccctggttc gcgatgagtg agaaggaaac
     3481 gtaatatttt aggcggcagt aaatccccca tttcccgggc tgaagagccc gtgcctttcg
     3541 acgacgacat ttttttaccg ccgacataga tccattcgta tggaacattg atgggaggtt
     3601 cgcgtttaaa cacttgtcga gaaattgctt cggcaatatc tcgcgggcct ccttttgtcg
     3661 agtgatcttt tccgcctccc tcaatgtcaa catcgaaaat tacaaatttt gacggccatt
     3721 caactttcca tggtaatttg gcttttccgt tgagcggaga aactcgtcca gtatgtccgc
     3781 accccacggc ccactcaaca tatttgccgc aactgtatgc aacagtctcg ccattccagt
     3841 ccgttgcctt cgttgtgcct actttgccac aattctcaca cactacggaa aggggcaacc
     3901 atgacggatc tctgtctgcg ccggatactt ccttataaat ctttctgatc gtcgcggcgt
     3961 tatcaagtat ttttcgaatg cattcgttgt atttaccgct cttatagacc tcgcttgacc
     4021 ggtaatattt tggcttgagt cctatttcct ccacaacttc tgtaaactcc tttgaaaaat
     4081 actcggcaaa gttcttggct tttccgtcgg gagaaggcac gttgcagagt agtttaccca
     4141 tatgaggccg gaaggttttt tcgtcgagat atccaggaag accatccatg acgtcaaaat
     4201 cgtttatttc ccataaaaat tcattcgcaa tgtttttttc gcccaacgcc tcaccaataa
     4261 ccccatgaat tgcagcgccg cgaagcgagc cgacatgaat acggccagaa gctgtctttt
     4321 catctcgaat gacaacaggt tttcccgctt tgattttgtc cttatagcgt cctttcgctt
     4381 cttcggcaac ttgatcagcc cagtacataa gtaaaattat caattaccaa gcttcaatta
     4441 tcaatgggga actcaattga aaattgatca ttgaaaattg gaaatttaca ttgaatgctt
     4501 attcaatgtt caccacctta tacttcatca cccctctcgg cgatttgaaa gaaaaggact
     4561 cacttttgcg cttgccaagc atggcctctc caagcggcga atgaattgaa agttttccgc
     4621 tcgagacgtc ggcctcttct gaacctacaa tttcaaattt atgttccttt ccatctgtgt
     4681 cgcgcgatac gacaacggtc gagccaatac cgactgcctc cgtatggtgc aactgcatga
     4741 tttcggcatt ttggagtatg ttttctagct tctgaattcg atcctcaata tttgcctgca
     4801 attcacgcgc ttcatgatat tcggcattct ccgaaagatc accgagtgat ttggcatatt
     4861 cgagatcctc tgccacttcc tttctcctga ctttacgaag atgatcgagc tccttcgcaa
     4921 gctcgtcgtg cttctccttt gataagtatt ctttttgatc tggcatataa aaaattacta
     4981 tagacttgat tcagcactat actaaaaaaa accacaaaaa tcaacgggca ggcgttcggc
     5041 cttcccaaac acagatattt gtgatatttt ccattttaga atgaaatacc aaacaactct
     5101 tagacgaatc cataacctcc cttgggaaaa cctggacaca ccgaaactcg aagatctcat
     5161 ggtcatatca ggaattacgg cacgagaata cacaaattcg ttacgaatcg cacgcacgct
     5221 acttcctgaa cataaggggc tccggctcct tacgcgagag gagtggcgga cctcaagttt
     5281 ccactttggc tcttattcaa gaaaaggaga tcgtgctgat ttccttcgcc actttgttaa
     5341 caaatccggt ctccttaaaa cagtaccgca tctcgttggt tctgcgtgcg tcacctacga
     5401 tttcaaaacg ggggcgctgc ctgacgacgt gagactgatg tctgttgtat cacgggagcg
     5461 cgacttaacc cgaatgatga ctcggctctt aagttccccg ccagagtgtt ggcaaggaga
     5521 agcgcttcaa gcattccgcc actatcttgc ccgtcgcata agacttcgcg agacaggaga
     5581 ggggcacctc gatcccacga gctcaatcag ggtgactaac tccgttctga agttttacaa
     5641 gttgcggctt gaactttatc aatccttgtt cgccgaccat aacacacaca gcccttaaag
     5701 gctgtctttt tttctctata tttgtcgcat aaaaaaacaa aaacccggaa ccaaaatcgg
     5761 tccaggccta aaaaataaac aaaaagaact aaagagtcgt tggcaggagg cgcggcgctt
     5821 tttccacgcc gcaccccccg ctggaagact tccgcaaaag aggagcgatt cagcttcgta
     5881 taaacgctcg ctcgaagcag aggacttcca cagtggccca caaccatgcg gtttggccac
     5941 cggcgcacaa tccatgcgcg caaaatttta gcgactaatc aaagaacagg gggttccgcg
     6001 cagg
//
LOCUS       DATDSU010000135         4256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2303487, whole genome shotgun sequence.
ACCESSION   DATDSU010000135 DATDSU010000000
VERSION     DATDSU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303487"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..684)
                     /locus_tag="VI981_04810"
     CDS             complement(361..684)
                     /locus_tag="VI981_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEY4479643.1"
                     /translation="MPAPEPPQAATSAPAIPAPASTSSPQATHAARDLLVKARATIQD
                     RKHKKLEKILEALTVKGKITNDEVEKLLHVSDATATRYLSALEKEGKIQQVGRTGKAV
                     EYTRI"
     gene            complement(1079..1861)
                     /locus_tag="VI981_04815"
     CDS             complement(1079..1861)
                     /locus_tag="VI981_04815"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HEY4479644.1"
                     /translation="MALSNEQIKSVEEVLKASLRNKFQNYKPEPASMPFHTRLLGKDR
                     LALYSFIHSLNTNFGTSIFEPVGLALAQKNFKMAISQATAGDQISTAAQAEIQKIIDG
                     LITATGTPNKKEEIERIRRVCQTGDMIKVKPTKVDLMFESKDGAYFLFDIKTAKPNAG
                     GFKEFKRTLLEWVAVVLANKPGAEIHTLIAIPYNPYEPEPYTRWTMRGMLDLENELKV
                     ADEFWDFLGGKNTYKDLLDCFERVGIELRAEIDDYFKRFSKK"
     gene            complement(1867..3441)
                     /locus_tag="VI981_04820"
     CDS             complement(1867..3441)
                     /locus_tag="VI981_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY4479645.1"
                     /translation="MALATIQEASQWASDLLNKDVSPTNISYLVQYGKVKKHGENGST
                     MVDLNDLKKYYDSYNGKREVSWKKNLGDDLNWVLSFDNLREKDTTKHVHRLHPYKGKY
                     IPQLVEYFIDNHIDNFKKEAYFEAGDIILDPFLGSGTTIIQSLEMGIHSIGIDVSEFN
                     CMISSCKATHYDDEYLQKAIKKLTASLDSFEHDNKIQDFENELLAELAKFNLVHFPGY
                     DFKYKVNQGGFNENKFAAEKEKEFLPTYQKLLKKYSIKLSQGKSNSFLDIWFTDNVRR
                     EIDHVFQTIKQEKDVKTSKILALILSRTIRSCRATTHSDLATLKEPQLTTYYCYKHKK
                     ICKPLLSITGMLNRYALDTLGRIREFNRLRKPVHYAVLTGDSRAVNILEKVEKQNPEF
                     SKILKRQKIAGIFCSPPYVGQIDYHEQHAYAYDLLGFKRKDDLEIGPLYKGQGQEARR
                     SYAQGIADVLNNCKQYLKEDFDIFLVANDKYNLYPEIAEKAGMKIVNQFKRPVLNRTE
                     RDRNPYSEIIFHFKII"
     gene            complement(3526..3729)
                     /locus_tag="VI981_04825"
     CDS             complement(3526..3729)
                     /locus_tag="VI981_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479646.1"
                     /translation="MAKDATGMRGYRSRNEDGQLRDKRDDTHIGTIEKQYDLDLGVRS
                     DMQLGTYLKEHNIASLNDLVNGK"
BASE COUNT          976 a          976 c          971 g         1333 t
ORIGIN      
        1 tgcgtccgtt cgcttcgtct ttgtatcggt ttcaggtttg ctagcgtaca gcgggtctca
       61 tttatccgcc gctccgttcg ggttcgtgcc tcggcttttg gagcctccta gcgcgctgca
      121 gctcccccca cccgcggtca gcatttcatt tccgattttt tagtacattg ttggtcatcc
      181 gcttgcgcgg gccggcctcg gtccagcttt ccctagcgag atacctgccc gcctgatggc
      241 atccatttta tgtatgcgcc tgtctcaggt caggcgcatg ggtcagccgc gcttctcgcg
      301 gctgcgcgtc gtttgtatat cgcatttcac ttccgatttc gtgcctcctt gcatgcaaat
      361 tcaaatgcgt gtgtactcga ctgcttttcc cgttcttccg acttgctgga tttttccttc
      421 cttttccaat gcagagagat accgcgttgc tgttgcgtcc gagacgtgta gcaacttctc
      481 aacttcgtcg ttggtaatct tacctttcac cgtcagcgct tccaggattt tttcgagttt
      541 cttgtgcttt cggtcttgaa tcgttgcgcg ggctttcact aagagatcgc gtgcggcgtg
      601 cgtggcttgc ggtgagcttg tcgaagccgg tgcgggtatt gcaggtgcgc tcgtcgccgc
      661 ctgcggcggc tctggcgcgg gcacattggg ctcactttgc ccttccgtag cctcgtgcga
      721 aggaggggtt cgggaggcaa cggctcactg gctggtattt gagccgtttg ggtttcagcc
      781 ggtggcattt ccaccggctc gctcggcgct ggttcgggtg ctgtagcggg ttctaccggt
      841 gctgttggca ctgagggagc ttgggatgtg tcttcgtctg ccatggctct attctagcgc
      901 gccggacggc ttgtcgggag taacgccgaa cggctcatat agttggcccc gaaatgcttg
      961 cttttttggt aggaagtggt acaatagaga ttacagtttc ttgagtgcac tcaagacctc
     1021 ctccacattg ccggtggtgc agggggttgg gatgtgtctt cttctgccat ggccgacttc
     1081 actttttact aaaccgctta aaatagtcat caatttcggc tctcaactca atacctaccc
     1141 tctcaaaaca atccagtaaa tccttatagg tatttttacc accaagaaaa tcccaaaact
     1201 cgtctgctac tttaagctca ttttctaaat ctaacattcc gcgcatagtc caacgggtat
     1261 aaggttctgg ttcataagga ttatatggaa ttgcgataag tgtatgtatt tctgcaccag
     1321 gtttgtttgc caaaacgaca gcaacccatt caagtagcgt gcgtttgaac tctttaaatc
     1381 caccagcatt tggttttgca gtttttatat caaacaaaaa atacgcaccg tcttttgatt
     1441 caaacattag atcaactttt gtaggtttta ctttaatcat gtcgccggtt tgacagactc
     1501 ttcgtattct ctcaatttcc tcttttttat ttggagtacc cgttgctgta ataagcccgt
     1561 caataatctt ttgaatttcg gcttgtgcgg cagtgctgat ttgatctcct gcagtagctt
     1621 gcgaaatagc catcttaaaa ttcttttgcg ctaaagctag accgaccggc tcaaaaatac
     1681 tcgtaccgaa attggtattg agggagtgaa tgaaagaata tagagcgaga cgatctttac
     1741 cgagcaaacg tgtatgaaaa ggcattgatg ccggctctgg tttatagttt tgaaacttgt
     1801 tgcgtaaact tgccttcaaa acttcttcaa cgcttttgat ttgttcattg gataacgcca
     1861 tatggtttat attatcttga agtgaaaaat tatctctgag tacggatttc tatctcgttc
     1921 cgttcggttc aataccggcc gcttgaactg attcacaatc ttcattcccg ctttttcagc
     1981 aatttctgga tagagattgt atttatcatt cgccacgagg aaaatgtcaa aatcttcttt
     2041 aagatattgc ttgcagttat ttagcacatc cgcaattcct tgcgcatatg accgacgagc
     2101 ctcttgtcct tgtcctttgt acaacggacc tatttctaaa tcatccttgc gcttaaaccc
     2161 gagaagatca taagcatagg catgttgttc gtgataatca atctgcccaa catatggcgg
     2221 cgaacaaaat atacctgcta ttttttgccg ctttagtatt ttggaaaatt ctggattttg
     2281 tttctctact ttttcaagaa tgtttactgc tcttgagtca ccagttaaga cagcataatg
     2341 gacgggctta cgcaatctgt taaattctct gattctgcct aaagtatcca gagcgtagcg
     2401 attgagcatg cctgttatgg agagaagggg cttgcatatt tttttgtgct tgtagcagta
     2461 ataagtcgtt agttgaggct ccttgagagt tgcaaggtcg ctatgcgtag tcgcacggca
     2521 cgacctaatc gttcgactca gaataagtgc cagaatcttg ctggtcttaa catccttttc
     2581 ttgttttatc gtttgaaaaa cgtgatctat ttcccgacga acgttatccg taaaccaaat
     2641 atcgaggaaa gaatttgatt taccttggct aagcttgatt gaatattttt taagtagttt
     2701 ttgataggta ggtaaaaatt ctttttcttt ttctgcggcg aatttatttt cattaaagcc
     2761 tccttgatta actttatatt tgaagtcata acccggaaag tgcactaggt taaatttcgc
     2821 aagttctgcc aaaagttcat tttcaaaatc ttgaatttta ttgtcgtgct caaaagaatc
     2881 aagcgaagca gtcaattttt taatcgcttt ttgcaaatat tcgtcgtcat agtgggtcgc
     2941 cttacaactt gagatcatgc agttaaattc ggaaacatct atgccgatgg agtgaattcc
     3001 catttcaaga gattggataa ttgttgtccc ggaacctaga aacggatcga gaataatgtc
     3061 accggcttca aaataggcct ctttcttaaa gttgtcgata tgattatcta taaaatattc
     3121 gaccagctgt ggaatgtact tgcctttata ggggtggagt ctgtggacat gcttggtagt
     3181 atctttttct cgcaaattat caaaagaaag tacccaattt aaatcatcgc caagattttt
     3241 tttccaactc acttcgcgct tgccattata cgaatcataa tattttttaa gatcattcaa
     3301 atcaaccatg gtcgaaccgt tttctccatg cttttttact tttccatatt gaactaggta
     3361 agagatgttc gtaggcgaaa catctttgtt aagaaggtct gatgcccatt gagaagcctc
     3421 ttgtattgta gccagcgcca ttcggacatt ataccccact cctaaatctt cattgtagaa
     3481 aacggcgaac agcccacttt cgtgggctgt tcttgcgcac ctcctttatt taccgttcac
     3541 gaggtcgttc agtgaagcga tgttgtgctc tttgagataa gtgcccaatt gcatatcact
     3601 gcgaactcca agatcgagat cgtactgctt ttcaatcgtg ccgatatgag tatcgtcacg
     3661 cttgtctcgc agttgcccgt cctcgttccg gctgcggtat ccccgcatcc ctgttgcgtc
     3721 ttttgccatg cgcgattttg cggatgttga taacccgctt gctaccttag aacaccgcgc
     3781 ttaaactgcg caggtgcttt aaagcccgtg ttgctctagt cgactcgaaa gttttgtttt
     3841 ctttgtttgc atagagacgc gggaaagtaa atttggttga agtcgcggga ctcgagagag
     3901 tgccgcttct tcatatttca ccgtatcata cggtgagcac atttttgtag tcatagaaaa
     3961 gtggataact ttttaaaccg cccccgcccc gccgcccatt caattttcaa aaaaccccgc
     4021 gaaatagatg tgtatggagg agtgtgcggc tgggcggggg cgaggacgcg ccagcaggcg
     4081 cgtccgagag agtcagcacg aagtcgaatc ggaccactcc cgtacaaagc taccacgcgc
     4141 gcggagcgcg cgacatcagg ggttcagggt gaaataggta cgagccaatt cgtaaagagg
     4201 agcaaggagc tggtaagggt ctgaaaaagc gggattttgg cgcgcggcgc gaagcg
//
LOCUS       DATDSU010000136         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2154822, whole genome shotgun sequence.
ACCESSION   DATDSU010000136 DATDSU010000000
VERSION     DATDSU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154822"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VI981_04830"
     CDS             complement(<1..219)
                     /locus_tag="VI981_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479647.1"
                     /translation="MKTYIKSQVHAWTALSLISLCVLSVALSAHAQVFDHPLSLGTEG
                     EDVTLLQRLLNTAGFTVAPPPEAGSPGNE"
     gene            complement(284..670)
                     /locus_tag="VI981_04835"
     CDS             complement(284..670)
                     /locus_tag="VI981_04835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY4479648.1"
                     /translation="MKKDFNKWNKKKKYIHAQAENKLYHEREVWWCALGVNIGYEQDG
                     TGDGGERPVLILKGFSRQVCLAIPLTTSKKKNPYHISLGIIGGKEASVIISQIRLLDT
                     KRLINKIGVVDHKLFEQIRKSAKDLL"
     gene            complement(844..1608)
                     /locus_tag="VI981_04840"
     CDS             complement(844..1608)
                     /locus_tag="VI981_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY4479649.1"
                     /translation="MTIVYSLIAVFVISLLAFSGIVFLPFKELSLRKVIFWLVSLSVG
                     AMLGDSMIHLLPKAFEAASSLSVAFSALFGILLFFMLEKFMHWRHSHGVDEETAETEL
                     AHKHADNQHVGMLLNVADGVHNFIDGLIIAGSFIVSTELGIATTVAVILHEIPQEVAN
                     FGLLIHMGYTRRKALFWNFISALTAFLGVGIAFAFNTEKMMAVLVAFAAGGFIYIALS
                     DLVPELQSVRGKASNVLQVVGILLGIGLMALLLLFE"
     gene            1731..2615
                     /locus_tag="VI981_04845"
     CDS             1731..2615
                     /locus_tag="VI981_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY4479650.1"
                     /translation="MPSDNNSTQENTSSEAKADSRESQKKDSPIAIPGAIVLAGLLIG
                     AAILYSAKDTSTVTKPTIQSSAIEIADVTPNDHILGNANAKIVLIEHSDTECPYCKVF
                     HAVMHKLVDTHGKNSELAWVYRHFPLYKGATDRPPLHPKAGKEAEATECVNELAGSAA
                     FWTFVDKIYETTPSNNGLDLAVLPQLAEDAGVPRAAFTACLDSNKYTQFVESSYTASI
                     KAGGTGTPYSVIILKKPLNEKGKQKMLEDAQKAFLTLNRNAYLPDDLFRFDESGTKIG
                     FSGALPFEVMEAVIKALL"
BASE COUNT          823 a          644 c          619 g          702 t
ORIGIN      
        1 ttcgttgcca ggtgaacctg cttcgggtgg aggagccaca gtaaagcctg cggtgtttag
       61 gaggcgttgg agaagggtga catcttctcc ttctgtgccg agagagagag ggtggtcaaa
      121 cacctgcgcg tgggcgctta aagctacact tagtacacaa agagaaatga gggagagggc
      181 tgtccatgca tgcacttgac tttttatata ggttttcata gtattaattt acaaatggct
      241 tcggccttgc cctccgcaag gagggagaaa ataagaaaat ccgtcagagc aaatctttgg
      301 cggattttct tatttgctca aacaatttat gatcaacaac tccaatcttg tttatcaaac
      361 gctttgtgtc taaaagacga atctgcgaaa taataactga tgcttctttt ccacctatta
      421 ttcccaggga aatatgatat gggtttttct ttttggatgt agtaagcgga atagcaagac
      481 aaacttgtct gctaaaccct ttaagaatga gcacgggtcg ttctcctccg tcacccgttc
      541 cgtcttgttc atatccgatg tttaccccca aagcacacca ccagacttcc ctctcatgat
      601 aaagcttgtt ttcagcctgt gcatgaatat actttttctt tttattccac ttgttgaagt
      661 cttttttcat gtgtctgatt attatactcc aaggttccgc cttttcattc gcatgcccta
      721 ttcgtaaggg ttctcaactt aaggtcgaac cttgagaaga ttagcggcct gaattgtaga
      781 gttgctggat ttcccccgcc gacagcgcgc ggcctgcccg ccacagcttg agtgcgcgag
      841 tgactattcg aaaagcagta gaagcgccat gagaccaatg ccgagcaaga ttcctacaac
      901 ttggagcacg ttcgatgcct ttccgcgtac gctttgaagc tccggaacaa gatctgataa
      961 agcaatatag ataaatccgc cggccgcaaa ggctacgaga accgccatca ttttttcagt
     1021 gttgaaggcg aatgcgatac caacaccaag aaacgcagtc agtgctgaaa taaagttcca
     1081 aaaaagagcc ttacgcctcg tataacccat atgtattaag aggccgaaat tcgccacttc
     1141 ttgaggaatc tcatgcaaga tcacggcgac tgtggtcgca atgccgagct ccgtgctcac
     1201 aataaaactt cccgcgatta taagtccgtc gataaaattg tgcaccccgt cggcgacatt
     1261 gagaagcatt ccgacatgtt gattgtcggc atgtttatgt gcgagctcgg tttcggcggt
     1321 ttcctcatcc acgccgtgcg agtgacgcca gtgcataaat ttttccagca taaaaaaaag
     1381 caatatcccg aaaagggcgg agaacgcgac actaagagaa gacgcagcct caaatgcctt
     1441 cgggaggaga tgaatcatgc tgtctccaag catcgctccc accgaaagtg agacaagcca
     1501 aaagatgact tttcgaagtg ataactcctt gaacggcaaa aaaacaatac ccgagaaagc
     1561 aagtagacta atcacaaaaa cggcaattag tgaatacaca atggtcatgg attgaaacta
     1621 taccacatat cagaacgtcg tcgacagcgc aaatttccac ctgtataaat gatggagttt
     1681 ggatatgggc tgagacatag tatgatatcg agatattaaa ataaaatcac atgccatcag
     1741 ataataattc gacacaagaa aatacttcaa gcgaagccaa agccgattca cgcgaaagtc
     1801 aaaaaaaaga tagcccgatc gcgataccag gagcgattgt tttggcgggg ttattgatcg
     1861 gcgccgcgat actatattca gcaaaagata cgtccaccgt taccaagccg accattcagt
     1921 caagtgccat tgaaattgcc gacgtcacgc cgaatgacca cattttaggc aacgctaatg
     1981 caaagatagt tttgatcgaa cattctgaca ctgaatgccc ttactgcaag gtttttcatg
     2041 cggtcatgca caagcttgtt gatactcacg gaaagaatag cgaactggcc tgggtttatc
     2101 gccactttcc actttacaag ggcgctacag acagaccgcc acttcaccct aaggccggaa
     2161 aagaagccga ggcaaccgaa tgtgtaaatg aacttgctgg ctctgccgca ttttggacat
     2221 tcgtcgataa aatttacgaa acaactccgt caaataacgg acttgacctt gcagtgcttc
     2281 ctcaacttgc ggaagatgct ggtgtaccgc gcgccgcatt tactgcttgc cttgacagta
     2341 acaaatatac tcaatttgta gagtcgagct atacagcgtc cattaaagca ggaggtacag
     2401 gtacaccata tagcgtaatt atcctgaaaa agcccctgaa cgaaaaaggt aagcagaaaa
     2461 tgctcgagga cgcacaaaaa gcctttttga ccctaaaccg aaacgcctat ttgccggacg
     2521 atctttttag attcgacgaa agcggaacta aaataggctt tagcggagcg ctaccatttg
     2581 aagtaatgga agccgtcata aaggcacttt tatagagtct cgcaggttta ttttggctag
     2641 cgaggagcgg gtgaacgtgt cttcacgttc acatcgtccg agcgaagaca aaatatatga
     2701 ttggagtcac atataattgc gtcattcgct cgaaatgaat atgaagacat attcatttct
     2761 ctctcactag caattatagg tctagtac
//
LOCUS       DATDSU010000137         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_126377, whole genome shotgun sequence.
ACCESSION   DATDSU010000137 DATDSU010000000
VERSION     DATDSU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126377"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /gene="hemH"
                     /locus_tag="VI981_04850"
     CDS             <1..515
                     /gene="hemH"
                     /locus_tag="VI981_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY4479651.1"
                     /translation="PTDFRVTYVDSWHDNPLLIECIGEQTKDALKKFDDKNTVEIIFT
                     AHSLPSRIEEWNDPYEKELLATSKLVAQKIAHHKWRFAYQSAGHSSEPWLGPDILDTL
                     SEIRNEGKKDILVVPIGFVSDHLEILYDIDIEAKTEANKLGLNLMRSQSRNTHPIFIK
                     ALASVVVTQS"
     gene            535..1317
                     /locus_tag="VI981_04855"
     CDS             535..1317
                     /locus_tag="VI981_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479652.1"
                     /translation="MEGFENPESENIAERVALKYPKFKAAIYAMTAALMLGGEPSRDA
                     SIETGERTETLDTIAQKADPHFREVAQSIVGEIKNFSSEQPTNNTYIHRLNYVSVGSL
                     KAYEEQSKGELGDEGENMLVGSDSTSRLYGGSYVDGTNENKIMIDGLSSNFYISAANG
                     ELNEGGKITMRGEGESKVEAIQNALESGINFLGTKVETATHSNKKTAMSPSLEVDESL
                     NEVVTTRGSEFVKHYKVTAVHGGDGRPFTVSVDMIVGVPEKK"
     gene            1319..1606
                     /locus_tag="VI981_04860"
     CDS             1319..1606
                     /locus_tag="VI981_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479653.1"
                     /translation="MKLVHKLNSAVLNNRFLQDVEKQMLLAHIATLPPSNQDQLALVL
                     PQKPKLLRVYYNLVSRVKDVGYLSGDQFEKTFDETLGEGEAEKDESLNEGN"
BASE COUNT          754 a          496 c          595 g          631 t
ORIGIN      
        1 ttccaacaga ttttcgcgta acatacgtcg actcatggca cgacaatccg ctcctcattg
       61 aatgtatcgg tgagcagaca aaagacgccc taaaaaaatt cgacgataaa aatacggttg
      121 aaattatatt taccgctcac agtctgcctt cgagaataga agaatggaat gatccatacg
      181 agaaggaact gctcgcgact tcaaagctgg tagcccaaaa aatcgcgcat cataagtggc
      241 gctttgcgta ccaaagtgct ggccactcga gtgagccgtg gctcgggcct gatattctgg
      301 acacattgtc agaaataaga aacgaaggca aaaaagatat actggtcgta ccgataggat
      361 ttgtttcgga tcacctcgaa attctttacg atattgatat tgaagcgaaa acggaagcga
      421 ataaactcgg gctcaatctc atgcggtcac aatctagaaa tacgcacccg attttcataa
      481 aagcgctggc gtcggtcgtt gtgacgcaaa gttagaggtg atatgatttt ggacatggaa
      541 ggattcgaaa accccgaaag tgaaaatatc gcggaacgag tggctctaaa atacccaaaa
      601 ttcaaagcgg ccatttacgc aatgacggcc gcccttatgc ttggaggcga gccttctcgc
      661 gatgcatcaa tagagaccgg cgaaaggaca gaaactctgg acactatagc gcaaaaagca
      721 gatccgcatt ttagagaggt ggcacaatcc atagttggtg agattaaaaa tttctcttca
      781 gaacaaccta caaataacac gtatattcac cgtctgaact atgtatctgt cggttccttg
      841 aaagcatatg aagagcagtc caaaggtgag cttggtgatg agggggaaaa tatgctcgtt
      901 ggttccgact ctacgagcag actatacggt ggttcctatg ttgatggcac caacgaaaat
      961 aagatcatga tagatggtct ttcatctaat ttttatattt ccgccgcaaa cggcgaactt
     1021 aacgaaggag gaaagataac gatgcgcggt gagggtgaga gcaaagtgga agcgattcag
     1081 aatgcacttg aaagtggtat caattttttg ggaacgaagg tagagacggc aacacattcg
     1141 aacaaaaaaa cggcaatgtc accgagtctt gaagtagacg agtcacttaa tgaagtagtt
     1201 acgactcgcg gcagtgagtt tgttaaacac tataaggtaa cggcagttca tggaggcgat
     1261 ggccggcctt ttacagtttc cgtggacatg attgttggag ttccagaaaa aaaataacat
     1321 gaaacttgtt cataagttaa actccgccgt actaaataat agatttcttc aggatgtcga
     1381 aaaacagatg cttttagcgc atatcgcgac cctgcctcca tctaatcaag atcaattggc
     1441 gcttgttctt ccccaaaagc ccaaactcct gagggtgtat tacaatcttg tttctcgggt
     1501 aaaagatgtc ggctaccttt ccggcgatca atttgaaaaa acgtttgatg agacattggg
     1561 tgaaggggag gcggaaaaag acgaaagtct caacgaagga aattaaaaat ctccccgtta
     1621 aaatggggga ggaagaaaga ttggttgcga atctctgcgg cgcgaaaaaa atttcccgat
     1681 ggcttgtatg gccgctctta caagatgaag caatttttca tacttatatt agaataatta
     1741 attcgtaaaa ttgtgctagg aataactttg aaaatcgatg gtccttccga aagctggcgc
     1801 gcaacaaaat tagttattgt ccgtctttac tatcaatatc tgcggtatta acaataatgg
     1861 cttgcgggtg tagcatatcg ctagcggtca actcacattg gaggaatgtt tatggaattc
     1921 tttacagtgt tctgttgttt ttgcaatgaa ccagctcctt atagttcggc agactatgtt
     1981 caggagggcg tctactcgtg tacaaagtta aggtgccgga gattgtgcaa atgttttcgc
     2041 ctcaaaaaat actttccgac ccttcccaaa cttgagggaa tgagtcgacg gcaagcagct
     2101 tagcttgctg tttttttatt ttggctagcg agcggcggca tgtaagttta ctttcataac
     2161 gcgcgagcga agacaaaata cgagacgagt gaaatgagtg ctcttatttt ggctagcgag
     2221 gtgagaagct ccgggggagc ttcgcaagac cttttgagct cgagtcttcg agagcaaaag
     2281 gtgtacagct tctgtaagaa gcgggtgaat ccgtcagccg acggattcac atcgtccgag
     2341 cctcgcccgt accgaacggt acgttcgggc agggaagaca aaatatatga ttggagtcac
     2401 atataattgc gtcattcgtt cgaaatgaat atgaagacat attcatttct ccctcactag
     2461 caattacatg attgga
//
LOCUS       DATDSU010000138         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1970532, whole genome shotgun sequence.
ACCESSION   DATDSU010000138 DATDSU010000000
VERSION     DATDSU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970532"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..1521
                     /locus_tag="VI981_04865"
     CDS             505..1521
                     /locus_tag="VI981_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479654.1"
                     /translation="MKKKHEKSSCCRGQVRRFGGRRRQCVMCKKTWRVWKTRRGRKKL
                     RVSVEIAHRFVMHRLLPVRSPQAGPMLSRNERAYRLGQSRTRCASLCPWPIVPEGNLI
                     AVADALVKYVEGTWHTWYFVLVRALEETEATVLPPFHRIGTETSTGWCEAFDALNAPI
                     RNRIKALVCDGHTGLVYEARWRGWLLERCHFHLIARIQSRRSKWKTSQHYEEGRHLYC
                     LVKRVLTESNERQLQEVVNELEEIGWETPSKDLKNTLKGFVNHYQEFRTYLYHPQLRL
                     PITNNTAETFIGLVEEVGSRARGFKNVSVLNEWITCVCKTRKKIRCAPPEEKKKYINR
                     YNQT"
     gene            complement(1814..2086)
                     /locus_tag="VI981_04870"
     CDS             complement(1814..2086)
                     /locus_tag="VI981_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479655.1"
                     /translation="MVTIYPLLNKINDIILNPLIVLGFSMALVAFFWGLFQFVSGASV
                     EEVRTKGKSNMMFGLLGFFIMIGVYGIIQIILNTFGIKGPAYLKLN"
     gene            complement(2156..2560)
                     /locus_tag="VI981_04875"
     CDS             complement(2156..2560)
                     /locus_tag="VI981_04875"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479656.1"
                     /translation="MKKITTVALVATFVTLAVAVPMVAFAQPTPPGTGNLSNLEVLVE
                     SVRRIVNMLIPLAFAIALLVFFWGLAKFILASGNEEAKDTGKRIMIWGIIALFVMASV
                     WGIVAFIGNALGIGQGQNIPSVPGVIDRDAGF"
BASE COUNT          862 a          662 c          659 g          714 t
ORIGIN      
        1 cttcttagtt tgcacaacgt ccgagccccg cccgtaccga acggtacgtt cgggcgggga
       61 agacaaaata tatgattgga gtcacatata attgcgtcat tcgttcgaaa tgaatatgaa
      121 gacatattca tttctctctc actagcaatt ataggtctaa taccgtccgc ctcaggcgga
      181 cggtggacga gcgaagcgaa ttcctatgtt accccgcggt ctttgccgca ggggcctata
      241 tatatttacc ccgttagaag cagaacgcaa tttgatttta gcgaacacgg ggtttttaat
      301 gcccgcgtga gcctctaacg gggtttactt tcggaaataa actccaatct tatttaaatt
      361 aaaaaaagac ataaaaaaac tcttagactc actatactaa cttcatgcta tagccacttt
      421 gactgaaaaa tttggttgat ggggttacta caataccttt caaatgaagg gtatctcgtc
      481 tttttgtagg acactttctt gtgtatgaag aaaaaacacg agaaatcctc ttgctgtcgg
      541 ggacaggtgc gccgcttcgg cggtcgcaga cggcaatgtg taatgtgtaa aaagacctgg
      601 cgggtatgga aaacgagacg cggaagaaag aagcttcgag tctctgtcga gattgcacat
      661 cggttcgtga tgcaccgctt gcttccagtt cgatcgcctc aagccgggcc aatgcttagc
      721 agaaatgaac gagcgtatcg tcttggacaa tcgagaactc gatgcgcaag cctctgcccg
      781 tggcccatcg tgccggaagg aaatctaatt gcggttgccg atgccttggt gaagtacgtc
      841 gagggtacgt ggcacacctg gtattttgtc ctcgtgagag cattggaaga aactgaagca
      901 accgttctgc cgccgttcca ccgcatagga acagaaacat caactggttg gtgcgaggca
      961 tttgacgctc tcaacgctcc gatcaggaat cgtattaagg cccttgtatg cgatggacac
     1021 accggtttag tgtatgaagc caggtggcgc ggctggctct tggagcggtg ccattttcac
     1081 ctcattgccc gtatacaaag cagacggtca aaatggaaaa caagccaaca ttacgaagaa
     1141 ggaaggcatc tctactgttt ggtaaagcgc gtgctcaccg aatctaatga gcgacagctt
     1201 caagaagttg tgaatgagct tgaagaaatt ggatgggaaa cgccgtctaa agatttgaaa
     1261 aatactttga aaggttttgt gaaccattac caagaattcc gcacatatct ctatcatcct
     1321 caactccgtc ttcccatcac caacaatacg gccgaaacat tcattggttt ggtcgaggag
     1381 gttggaagtc gtgcacgagg attcaaaaat gtttccgtgc taaatgagtg gattacctgc
     1441 gtttgcaaaa caagaaagaa aattcgctgc gcgccgccag aagaaaagaa gaaatatatc
     1501 aaccgatata atcagacatg accgccatag catgaagtta gtatactagc aaaaataata
     1561 tgacaagtga tttagaaact ttatgcaagc aatcgagatg taaattatcc gataaaaaat
     1621 aaactacccc gcggcaagcc gcggggaaat cgcttcgcgt attataagta caaaatggcg
     1681 tcatcccgga ctcgagtcca gtttccttta aactgcgagc ggaggcttgc gatacatcat
     1741 tacgcattca accccgcaca taagtgcggg gttgaatgcg taagaaacaa tcgatcaccg
     1801 ggatggtgct cggttaattt aacttgagat aagcagggcc tttgattccg aaggtgttga
     1861 gaataatctg aataataccg tacacgccga tcatgataaa gaaacccaaa agcccgaaca
     1921 tcatattact cttccctttt gtccgcactt cttcaacact tgccccagac acaaactgaa
     1981 ataaccccca gaaaaatgca acgagcgcca tgctgaagcc gagaacaatg agcggattca
     2041 agataatgtc attgatcttg ttgagaagcg ggtaaatagt gaccatagag ttagaccctc
     2101 tgtgccatgg ctaatccttg gcacaggaat aaggttaaga gaggtaacga aaaatttaga
     2161 atcctgcgtc tcggtcgatt actcccggaa cagatggaat gttctgccct tgtccgattc
     2221 caagcgcatt gccgataaag gctacaattc cccagactga agccatgacg aagagtgcga
     2281 taattcccca gatcatgatt cgctttccag tatcctttgc ttcttcatta cccgaagcaa
     2341 gaataaattt ggccaacccc cagaaaaaca cgagaagcgc aatcgcgaat gcaagcggaa
     2401 tcagcatgtt gacgattcgg cgaacagatt cgacgagcac ctcaagattc gagagatttc
     2461 cggttcctgg cggcgtcggc tgtgcgaaag caaccattgg aacggcaacg gccaaagtaa
     2521 cgaaagttgc tacaagcgct actgtggtga tttttttcat gaaactaata aattactatt
     2581 aaaaagacga ccccacatga ctaataactt tatggtagtc atgatcattt taggctaaca
     2641 attgtccaat ttcaacggat aatgtggaca acccggtagt agattttaag gttatggtat
     2701 ctaaatatgt gaatacctag ccggtaagga tgtgtgtcaa cgaaagaagc gtcccagcca
     2761 tttctgtttc cacaggaaac gaccttaaaa ttcactaccg ggtggacaac cctcggaaca
     2821 gtttttatac atttttctcc atattatatt aaaatgcctc gcccacctta ccacttcctg
     2881 ccccagtatt gacccgc
//
LOCUS       DATDSU010000139         3874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_795115, whole genome shotgun sequence.
ACCESSION   DATDSU010000139 DATDSU010000000
VERSION     DATDSU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795115"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..428)
                     /locus_tag="VI981_04880"
     CDS             complement(24..428)
                     /locus_tag="VI981_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479657.1"
                     /translation="MEQPPSGERAPENRGEGELSFERAFSLAVALLDAVDTRNGFTEA
                     HGGFDTFAETWQHVEGKRKEYDVMEEVVLWARREFDEKVHDKKAFVLQLRAMGRKGFA
                     DRIASMFGVSSEVPKSSRGGIFSRFRKKREKE"
     gene            complement(555..833)
                     /locus_tag="VI981_04885"
     CDS             complement(555..833)
                     /locus_tag="VI981_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479658.1"
                     /translation="MKTYSQFKKVALKNKKIRRAYEALGPEFEVITVLIKRRLEKGFT
                     QRELARRVGTKQSAIARLESGTYNPSLDFLKKVTYALDARIRITVNER"
     gene            complement(858..1172)
                     /locus_tag="VI981_04890"
     CDS             complement(858..1172)
                     /locus_tag="VI981_04890"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY4479659.1"
                     /translation="MNIRFFDAQLERFIQSLDEFTIAKVLRTIDLLAAFGHAFTLPHS
                     KKVHTRLFELRIRGKQEVRIFYSFHKNEAVLLHGFMKKTNRIPKKEMRLALKKLIALD
                     EI"
     gene            complement(1255..1431)
                     /locus_tag="VI981_04895"
     CDS             complement(1255..1431)
                     /locus_tag="VI981_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479660.1"
                     /translation="MSIPPTKKAKHYPFKKLAALAGVAIVGGFVMYTTARHGPALGLR
                     LIKAVVDKVAKKKK"
     gene            complement(1612..2904)
                     /locus_tag="VI981_04900"
     CDS             complement(1612..2904)
                     /locus_tag="VI981_04900"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY4479661.1"
                     /translation="MSDQIVTILKRKLDGLSAYGYSITDPETRLNALKEELQFYVLDF
                     IYHHPEYSKWIMYGGSALRICYDLDRMSVDLDFEVNNKIDIDFLNELKEEAEKHFSKV
                     YGVDSSFLRVTITNNRGITLKFHVGALISGYASEWVHIKIDLNHFPAPAKVVTERIPQ
                     NHGQLSFVIKTYNLSALMASKIAAIFLRGTRGVGEAVYEEKGRDIYDLLWYMSKQIVP
                     DLDYLKAKNVEEAKDFQTLFTKLTVKMSNVSEENLKNDLTPLFLDPRYVANWLANWRD
                     TYFRLRDAYKIRTISKLERVRVFEDFRTDVFSFIFEYRTEEGEPVRIICNLSEYWIIF
                     KDGDIPTVIDNFISDHIEFSANGIGGNLVPQKKLKQYASLFYEKIEVYLEKIDHMVLG
                     DTITTKLVRITADNLNPKEQIVLRKQDLLKCDFDDLLK"
     gene            complement(2897..3514)
                     /locus_tag="VI981_04905"
     CDS             complement(2897..3514)
                     /locus_tag="VI981_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479662.1"
                     /translation="MKSKKNLLEQLRSLPYFGKSTVFQLGSQLGLKETSVNTYISRFL
                     KHKEILQLKRGLYISADFFAKNKNDISYSFYLANILRTPSYVSSWAALQYYNLTTEAI
                     HSITSITPKVTRDYKTKAGTFSYQSIKKEFFSDYSLVKGKFDFFIVSPSKALFDLLYF
                     RTRQFRGVRFEEIKKMVEELRIDIDEMSEDEQKKFYEKVKKHLHE"
     gene            complement(3689..>3874)
                     /locus_tag="VI981_04910"
     CDS             complement(3689..>3874)
                     /locus_tag="VI981_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4479663.1"
                     /translation="YDDRVEKTAGERFADADLIGIPYRVVISASTLAQKGVEVKERAK
                     PQTRLMTPAACIKMLTK"
BASE COUNT         1047 a          874 c          756 g         1197 t
ORIGIN      
        1 gccgtttttt tgttttagta agattattct ttttctcgtt ttttccgaaa gcgggagaaa
       61 atgccgcctc gggaactttt ggggacttca gacgaaaccc cgaacatact cgcgatccga
      121 tccgcaaaac ctttgcgtcc catagctcgg agctgaagga caaacgcttt tttatcgtga
      181 actttttcgt caaattcccg tcgggcccat aatacgacct cttccattac gtcatactct
      241 ttccgcttac cttccacatg ctgccatgtt tcggcaaacg tatcaaaacc accatgggct
      301 tcggtaaatc cattcctcgt atctacggca tcaagcaaag caactgctaa ggaaaaagct
      361 ctttcaaatg acaattcacc ttctcctcta ttttccgggg ctcgctcccc gcttggtggt
      421 tgctccatat ataaagtata tcaattattc ttctacatta tcaaattctc tccaaaaaat
      481 caaggaaacg aaacggctca tccgtttttg gggatgagcc gtttatacat tttagctatt
      541 tagcgaaata tggattaccg ctcgttcacg gtaatccgaa ttcttgcatc aagcgcatac
      601 gttactttct tgagaaaatc taagctcgga ttgtatgttc cggattcgag tcgtgcgata
      661 gcagattgct tcgttccaac acgccttgct aactcacgct gagtaaaccc tttttcgagc
      721 ctgcgcttaa taagtactgt aatcacttca aattcaggac cgagcgcctc gtatgcccgt
      781 cgtattttct tatttttgag tgctactttt ttgaactgtg agtaggtttt cataatgtac
      841 caagtataac gtatatgtta tatttcgtca agagctatca acttcttaag tgcaagacgc
      901 atttctttct tgggaatgcg atttgttttc ttcataaacc catgcagtaa aaccgcctca
      961 ttcttatgaa aagaatagaa aattcttact tcctgcttac ctcgaatacg caattcaaaa
     1021 agccgcgtat gcaccttttt actgtgcggg agcgtaaatg catgaccgaa cgccgcaagg
     1081 agatcaatgg ttcgcaatac ttttgcaata gtgaattcat cgagtgactg aataaatctt
     1141 tctaattgtg catcaaaaaa acgaatgttc ataaggtttt ggaaaggtac ttttacccta
     1201 tcaaactttg ctcaaaaaag cgaggaaata aaagagatcc ccgaagggac ctgcttattt
     1261 cttcttcttg gcgaccttgt ccaccaccgc cttgatgaga cgaagtccaa gcgcagggcc
     1321 gtgccgtgcg gttgtgtaca ttacgaaacc gccgactatc gcaacccctg caagtgcagc
     1381 gagttttttg aaaggatagt gttttgcctt cttcgtggga ggtatggaca acttttttga
     1441 aaatatacca tacttcctct agtcgtgatc gatccgcttt acatttattg atttcgaaag
     1501 ttcccttgag aagaatttcg aaatcagttc ggtagcccat ctcactcgtt tccttgtgag
     1561 aaaacttttt agaaactgat tgaatatatg cggtaaaaag gtcgttcata tttattttag
     1621 gagatcgtca aaatcacact tgagcaagtc ctgtttgcga agaacaatct gctctttcgg
     1681 attcaaattg tcagcagtta tgcggacgag ctttgtagtg atagtatcgc ctagcaccat
     1741 atgatcaatc ttttccaaat atacctcgat tttctcataa aatagcgagg cgtactgctt
     1801 taattttttc tgtggtacaa gattaccacc gataccattc gccgagaact caatatgatc
     1861 gcttataaaa ttatcgataa ccgtaggaat gtcgccatcc ttaaagataa tccaatattc
     1921 gctcaggttg catataatac gcaccggctc accctcttcc gtacgatatt caaaaatgaa
     1981 tgagaacaca tcagtgcgaa agtcctcgaa tacacgcacc cgctcaagct tcgaaatagt
     2041 tctaattttg taggcatctc gcagtcgaaa ataagtatca cgccaattag caagccagtt
     2101 tgctacatag cgagggtcga gaaataacgg agtaaggtcg tttttgagat tctcctcgct
     2161 tacgttgctc atttttaccg tgagtttggt aaagagggtc tggaaatctt tcgcctcctc
     2221 cacgttttta gctttaaggt aatcgaggtc aggcacgatc tgtttactca tataccagag
     2281 caaatcataa atatcgcgcc ctttttcttc atacaccgct tcgccaacgc cgcgcgttcc
     2341 acgcaagaaa attgcggcaa tcttacttgc cattagcgcg gacaaattgt aagtcttgat
     2401 tacaaacgat aactgcccat gattctgcgg tattcgctct gtcaccactt tcgctggggc
     2461 gggaaagtga ttgaggtcaa ttttgatatg tacccattca gaagcgtaac cagaaattaa
     2521 ggcaccaacg tggaatttga gcgttattcc tctgttgttg gttatcgtaa ctcttagaaa
     2581 actggaatca acgccatata cttttgaaaa atgtttttcc gcttcctctt ttaattcatt
     2641 tagaaaatcg atgtcgattt tgttattgac ttcaaaatcc aaatcaacgg acattctatc
     2701 aaggtcatag caaatcctga gtgctgagcc gccatacatt atccacttgc tatattcggg
     2761 atgatgatag atgaaatcta aaacataaaa ctgcaactct tctttaagag cgttcaatcg
     2821 cgtttccggg tcggttatgc tgtaaccata cgcagaaagg ccatcgagct ttctttttag
     2881 gattgttacg atctgatcac tcatggagat gtttttttac tttctcgtaa aactttttct
     2941 gttcgtcctc gctcatttca tcaatatcaa ttctcaattc ctcgaccatt ttttttattt
     3001 cttcaaaacg gacgccccga aactggcggg ttctgaaata caagagatcg aacaacgcct
     3061 ttgacggaga tacaatgaaa aaatcaaact tccccttgac gagcgaatag tcagaaaaaa
     3121 attctttctt aatcgactga taggagaatg taccggcttt tgtcttatag tccctcgtga
     3181 cctttggtgt tatcgaggta atggagtgta tggcttccgt tgtcagattg taatactgga
     3241 gcgcagccca cgaacttaca tacgatggag tgcgcaggat gtttgccagg taaaaagaat
     3301 acgaaatatc gttcttattt ttagcaaaaa agtctgctga tatatacagg cctcttttga
     3361 gctgaagaat ttctttgtgt ttcaagaagc ggctaatgta cgtattaacc gatgtctcct
     3421 taagccctag ctggctacca agctgaaata ccgtgctttt gccgaaatag ggtaatgacc
     3481 ttagctgttc taataggttt ttcttgcttt tcataaatat gacaatagtg tatcatatat
     3541 gaaatgagca gtcaacagct catatataac ccgcccaacc tttttgccgc ccattccaat
     3601 tcaaatcccc tcacaaaaaa tcgaggaaac gaaacggctc atccgccttg ggtggatgag
     3661 ccgttctttt cgttttggtt atctctcctt attttgtaag catctttatg caagctgctg
     3721 gggtcatgag acgcgtttgg ggttttgcgc gttcttttac ttcaacccct ttttgggcaa
     3781 gggtactcgc agagattact acgcgatacg ggatgccgat taaatccgcg tctgcgaatc
     3841 gctcgcctgc ggtcttctcc acgcgatcgt cgta
//
LOCUS       DATDSU010000140         4863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_129115, whole genome shotgun sequence.
ACCESSION   DATDSU010000140 DATDSU010000000
VERSION     DATDSU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129115"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VI981_04915"
     CDS             <1..1432
                     /locus_tag="VI981_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479664.1"
                     /translation="KNGEGLKLAAASAKKNGFDITFASFGENDRGERVAWRADVLTID
                     EPYTHDGKTNYAKRLGFQVTTQPMEILGDRSAVTRLELSNDTDATSSERWNEWVECID
                     PRNKDSMGNGGIDRILITTEGSQGSYDTVGPVTVLYDRPGAIFENGMLIRTDSQDERT
                     FTLGWNFPSITSTRERTHIDLEMAKAYIRYYFTHTTDERAVEHLLRSIQTHDIDARSI
                     SWSDDKFTKIVAVADSHLQKQDVDASWALPNMQDYPSQKLFQKVAKKLYPNKTLFSYE
                     YAKRKGITMGGSMRHIREDERLNVSEEDYLRLRNIFPSMESYLEDISKSKINMDPESL
                     SPLRNMVGGEIDRVAATINRIRRTPSSKPLLDYILQRSNTSMKELHSRLDALRPETSA
                     GKKNDVFVMADDSNADGLASDGVGLRISLLDIRDRKSVGRLLGVLDHELSHKMLAAND
                     YTYEFILLLFLLARDSLEGRHYPEPL"
     gene            complement(1550..2761)
                     /locus_tag="VI981_04920"
     CDS             complement(1550..2761)
                     /locus_tag="VI981_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479665.1"
                     /translation="MFEKLHTNGREDSEELPAEFDFHQERFQKMADIHEQKWWVKAQK
                     IIMKQILDRDAVELSAVDFAHEEALRDDVDFERKRDEEEYEASERNLKPVIEALIQKR
                     NNPERKDIRTLLVIPGGGMRCAVGAGQALIGLNAVGMDKAFDNVFGISGGGQVAAFFA
                     GGKERSARGASIFYEECTYGEFIKKTRISRIMNVDVVSNAMTSGEKELDQKAVRDSGI
                     GLYLGVTRVDDEKCEIINAKTARPGIMDALKATMAVPLFYGKDIEVNETKYMDGAFDP
                     LPIQRLIEKFEPTHILILANVPFNRVDSFELSPGEYLLAEFLPKVGSLGTMAKFASNK
                     KQMRESLEFIQKQSGVNIGILWPPDTNLDTLTTDPYKIEASELEAARGVIKAFGGEQP
                     RDLNLYHHENT"
     gene            complement(2824..3708)
                     /locus_tag="VI981_04925"
     CDS             complement(2824..3708)
                     /locus_tag="VI981_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY4479666.1"
                     /translation="MVKVLSIVNKISVLRERYYKSSVGKDALLKLISESEVSEQVYNS
                     NAIENSTLTLEETEKILLQIDLDRFISEREIFEAKNLARVVSYIDKRAKEQELSLNAI
                     LSLHTMLIANIRDDIAGRFRIGKEYVRVGSHIAPAPQEIIERLEKMLTEYNSASHESV
                     IKRVARLHLAFEYTHPFIDGNGRIGRVLNNYVLIREGYVPINIKFIDRQKYYDAFTEY
                     NKNGVTKIMEEIVAKALTNSYHKRLAYLENKKIITLGEYAKCNKLSHPNLINKANRQT
                     IEAFLEKGIWKIPESFKP"
     gene            complement(3862..>4863)
                     /locus_tag="VI981_04930"
     CDS             complement(3862..>4863)
                     /locus_tag="VI981_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4479667.1"
                     /translation="KGDKPLPRLVRDRQMTAPTPALDLADVKGQESAKRALEIAAAGR
                     HNIVFYGPPGTGKTMLARALAGILPPLTDDDMLEVTAIHSTAGLLANGEAVYWPPFRS
                     PHHSLSHVAMIGGGSFPKAGEVTLAHKGVLFLDEFPEFESRALEALRQPLEDRIVTVS
                     RARATITFPADCMIVAAMNPADTLSNDFRASQRAAMKQARKISRPIVDRLDLWIEVPL
                     VPHDTLAKLSTGEGSAIVRERVVAARSRAEKRTGKKGMTNAALTSRQLDEKSGFSTAA
                     KETLMSAASRLNLSPRSFHRTMRVARTIADLAGADSVTPAFVHEALQYRPRGLFGFE"
BASE COUNT         1245 a         1130 c         1117 g         1371 t
ORIGIN      
        1 aaaaaacggg gaggggttaa aattagcagc tgcatccgcc aaaaaaaatg gatttgatat
       61 cacgtttgct tctttcggag aaaatgaccg cggagagagg gttgcctggc gggctgacgt
      121 actgactatt gatgagccat acacccacga cggtaaaacc aactatgcaa aacggcttgg
      181 tttccaagtt acaacacaac caatggaaat tctcggcgac cggtcggccg tcacaagact
      241 cgagttgtcg aatgacacag atgctacaag tagcgaacgg tggaacgagt gggttgagtg
      301 tattgatcct cgcaacaagg attctatggg taatgggggc attgatcgta tccttatcac
      361 cacagagggc tcgcaaggtt catatgacac ggtgggtcct gttacggtgc tttatgatcg
      421 acctggagca atttttgaaa atggtatgct cattcgaact gactcgcaag acgaacgtac
      481 gtttacgctc ggctggaatt tcccgtcaat cacgagtaca cgtgaacgta ctcatataga
      541 tctagaaatg gctaaagcat atatacgata ttacttcacg cataccacag atgagcgcgc
      601 cgtcgaacat ctactgagat ctattcagac tcatgatatt gatgcgcgta gtataagctg
      661 gtcggatgac aaatttacaa agatagttgc agtggctgat agtcacttac aaaaacaaga
      721 tgtggacgca tcgtgggcac tgccaaacat gcaggattat ccatctcaga aattatttca
      781 aaaagtagcc aaaaaattgt atcccaacaa aacacttttt tcttatgaat acgcaaaaag
      841 gaagggaatt actatgggtg gcagtatgcg gcatattcgt gaagatgaac gtttgaatgt
      901 atcagaggaa gattacttaa gacttcggaa tattttccca tccatggaat catatctaga
      961 agacataagt aagtcaaaaa ttaatatgga cccggaatca ctttccccgc tacgcaacat
     1021 ggttggtggt gaaatagatc gcgttgctgc taccatcaat cgcataagac gaactccgag
     1081 ctcgaaacct ttacttgatt atattctaca gcggagcaat acatcgatga aagaacttca
     1141 ttcgcgttta gatgccttac gcccagaaac tagtgctggc aaaaaaaatg atgttttcgt
     1201 catggcagac gattctaatg cggatgggct cgcgagtgat ggtgttggat taagaatatc
     1261 acttctcgat attcgcgaca gaaagagtgt tggcagactt ttgggtgtgt tagatcatga
     1321 gttgtctcat aaaatgcttg ctgcaaatga ttatacatat gagtttattt tgctgctctt
     1381 tcttttggcc cgagatagct tagaggggag gcattacccg gagcctctct agtcgacagc
     1441 cactgacacg agagacattt tcattcaaaa ccgaataacc ccctaggacg gtactggagt
     1501 gcttcgtgga cgaaagccgg cgcaaataca taaatttcat agtgcaaccc tatgtgttct
     1561 cgtgatgata gagatttaag tccctgggct gttcgccgcc aaacgctttt ataacacctc
     1621 tggctgcctc taattcagat gcttcaattt tatatgggtc tgttgttaaa gtgtctaaat
     1681 tagtgtcagg gggccagagt atcccgatgt taacgccact ttgtttttgg ataaattcta
     1741 gagattctct catttgtttt ttattgctcg caaacttcgc catagtccct agggaaccga
     1801 ctttgggtaa aaattcagct aacaagtatt ctccgggact cagctcaaat gaatctacac
     1861 gattaaaggg cacattagcc aaaattagaa tatgggtcgg ctcaaatttt tcaatcaatc
     1921 tttgtatagg taacggatca aaagctccat ccatgtattt cgtttcgtta acttctatat
     1981 cttttccata aaataatggg actgccatgg ttgccttaag agcgtccatt attcctggcc
     2041 ttgcggtttt ggcatttatg atttcacact tctcgtcatc aactcttgtg actcctagat
     2101 acaatcctat tcctgagtcc cggacagcct tctggtccaa ttctttttca ccgctggtca
     2161 ttgcatttga cacgacatca acattcatta ttctggaaat tcttgttttc tttataaatt
     2221 ctccataggt gcactcttca taaaaaatgg aggcccctcg ggccgatcgt tctttcccgc
     2281 ccgcgaaaaa ggcggctacc tgtccaccac cagagatgcc gaaaacattg tcaaaagcct
     2341 tgtccattcc aacggcattt aatccaatca gagcttggcc cgcgccaacg gcacatctca
     2401 ttcctccacc cggaataacc agaagtgtgc gaatgtcttt cctttctgga ttattacgct
     2461 tctgaataag agcctcgatg acaggtttta agtttctttc tgatgcctca tattcttctt
     2521 catccctttt gcgttcaaaa tcaacatcat ccctcagtgc ttcttcgtgg gcaaaatcca
     2581 ctgccgataa ttcaactgca tctcgatcaa ggatctgttt cattattatt ttttgcgctt
     2641 tgacccacca cttttgttcg tggatgtcag ccattttctg gaagcgttcc tgatggaaat
     2701 caaattctgc tggcagttct tctgaatcct cgcgcccatt tgtgtgcaat ttttcaaaca
     2761 ttcactcaat agtatcattc aaacccgaag agaccccggg ggcggtattg aagcgcttcg
     2821 tggttatggt ttaaaactct ctggaatttt ccagattcct ttttctaaaa aagcttctat
     2881 cgtttgacga tttgctttgt tgataaggtt gggatgtgaa agtttattgc attttgcata
     2941 ttcgccgagc gtaataattt ttttgttttc cagatacgcc agacgcttgt ggtagctatt
     3001 ggtcaacgct tttgctacaa tctcttccat tattttggtc actccgtttt tgttatattc
     3061 ggtaaaagcg tcgtaatatt tttgtcggtc aataaacttt atattgattg gcacataacc
     3121 ctcgcgtatc aatacataat tatttagtac gcgcccaatg cgaccattcc catctataaa
     3181 cgggtgggtg tattcaaatg caagatgcag tcgggcgaca cgcttgataa cactttcatg
     3241 acttgcggag ttatattctg tcagcatttt ttctaatcgt tcaattattt cttgaggcgc
     3301 aggggcgata tggctgccga cgcgcacata ctctttacct atgcggaatc ttcccgcgat
     3361 atcatcgcga atgtttgcaa tcagcattgt gtggagcgag agaatggcgt tgagtgagag
     3421 ttcctgctct ttagcacgct tatctatata tgagacaact cgcgcgagat tttttgcttc
     3481 aaaaatctcg cgctcactaa taaaacgatc gaggtcaatt tggagcaata ttttttccgt
     3541 ctcttcaaga gtaagcgtgc tattttcaat ggcatttgag ttgtagacct gctcggagac
     3601 ttctgactcg gaaataagtt taaggagagc gtctttaccc acggatgatt tgtagtatct
     3661 ctcacggagc acactaatct tgttaactat agacagtacc tttaccattc cacctattgt
     3721 agttgcatta acgttttttg tcaacataat cgtgaaaaat ggtagaaaaa tcatctattt
     3781 tcacgattat gtttcaaacc agactgcgtt gttcgttatc ttccacgacc gttagagata
     3841 acgaacatca aaaaatgatg gctattcaaa accgaataac cccctaggac ggtactggag
     3901 tgcttcgtga acgaaggcgg gcgtgaccga gtcggcaccg gcgaggtcgg cgatagtgcg
     3961 cgcgactcgc atggtgcgat gaaaacttcg aggcgagaga ttgagacgag acgccgcgct
     4021 catcagggtt tctttagcgg cggtgctgaa gccgcttttt tcatctagct gtcgactcgt
     4081 gagcgcggcg ttggtcatgc ccttcttccc cgtccgcttc tcggcacgcg agcgcgcggc
     4141 cacgacgcgc tcgcggacta ttgcgctccc ctcaccggtc gacagtttcg cgagcgtatc
     4201 atgcggcacg agtggcactt ctatccagag gtcgaggcgg tcgacaatcg gtcgagaaat
     4261 tttacgcgcc tgcttcatgg ctgctcgctg tgacgcgcga aaatcatttg agagagtgtc
     4321 tgccggattc atcgcagcga caatcatgca gtcggcggga aaagttatcg tcgcccgcgc
     4381 gcgcgaaacc gtcacgatgc ggtcttcgag cggctgtcgc agagcttcga gcgcgcgcga
     4441 ttcaaattct ggaaactcgt ccagaaaaag aacgcctttg tgcgcgagcg tcacttcgcc
     4501 cgctttcggg aaggagccac caccgatcat cgcaacgtga gagagcgaat ggtgtggcga
     4561 acgaaacggc ggccaatata ccgcctctcc attcgcgaga agcccggcag tagaatgtat
     4621 tgccgtcact tcaagcatgt cgtcgtcggt taatggcggg agaatgccgg cgagcgcacg
     4681 tgcgagcatt gtcttaccgg tgccgggagg cccataaaaa acgatgttat gccgaccggc
     4741 tgcggctatc tcaagcgcgc gtttcgcact ctcctgccct tttacatctg ccaagtctag
     4801 ggcgggcgtc ggcgcggtca tctgccggtc gcgcacaagc cgcggaagcg gcttgtcgcc
     4861 ttt
//
LOCUS       DATDSU010000141         4554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_795586, whole genome shotgun sequence.
ACCESSION   DATDSU010000141 DATDSU010000000
VERSION     DATDSU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795586"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VI981_04935"
     CDS             <1..982
                     /locus_tag="VI981_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY4479668.1"
                     /translation="YIGETICENADSIPLPAICIDEPTISLNFLVNDSPFAGRDGKYV
                     TGRQLRERLARELETNVGLKVDFSPETNRVGKTPYFKVYGRGELHIAILIENMRREGY
                     ELQVSQPQVIIKEDAGVKMEPFEEVIIDVKEKLSSSVIQTLSERRGQLLEMKEHGGNV
                     RIIFEIPTRGLLGYRSQFIIDTKGEGILSSRFTAFKEYIGPIDRHSFGSMISMASGIA
                     LAYSLWNLQERGRIYVNPGTPVYEGMIIGNVTKGDNLDVNPTKNKQMTNVRNSGNDDA
                     ILLETPLLLTIERGMEIMAEDDFLEVTPKNVRLRKKYLRETDRTRAARSK"
     gene            complement(1107..1415)
                     /locus_tag="VI981_04940"
     CDS             complement(1107..1415)
                     /locus_tag="VI981_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY4479669.1"
                     /translation="MSKKLYVGGISYNTNNDGLRDAFSKAGTVEDVAILTDKMTGKSR
                     GFGFVTMADEAAQAAIDMWHGKELDGRTLTVNEARPMAPRSDRPSRGGSYDRGDRRSF
                     "
     gene            complement(1540..2361)
                     /locus_tag="VI981_04945"
     CDS             complement(1540..2361)
                     /locus_tag="VI981_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479670.1"
                     /translation="MAKMNIVVYDKNMRDTVYNVYKKIGETPLACIQRLRVTYPELKN
                     KKLTYAGRLDPLAEGVLIILADESIKRKKEYLSLPKEYKFDILFGVATDTHDILGKIT
                     KLSLPTEILPESLKSILLALIGSATQTYPAYSSKTVRGKHLFEWARGDRLTEIDIPNR
                     QVEIFSLDIVGSYVLTSAALANFIADRISKVHGDFRQDEIRQLWKLNFARIGSCRHHI
                     YTLKVSCSSGTYVRALVEKLALLLDTSAVALHIKRVSVGDYTLKQSLKLPVPLPE"
     gene            2414..3646
                     /gene="hisS"
                     /locus_tag="VI981_04950"
     CDS             2414..3646
                     /gene="hisS"
                     /locus_tag="VI981_04950"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY4479671.1"
                     /translation="MAKVATEPYKGVRDFYPEEFAVEKYIWKKWVQVAESFGYVEYNA
                     SILEPSELYRGKTSEEIVNEQTYTFTDRGDREVTLRPEMTPTVARMIAAKRRELSFPL
                     RWFSIPNVFRYERPQRGRLREHWQLNADIFGVPGIEAEVEIISLAYYIMKAFGAKDND
                     FTLKIGSRASLNDLARRQNLTEEQFKKLIGLLDRRNKITPAEFHTGISELGFTLDILS
                     LQDPLADVPRICDLLKDKGIKNAIYDPSIVRGFDYYTGTVFEVFDTNPKNNRSLFGGG
                     RYDNLLDLFGEEPAPAVGFGMGDVTIRDFLETRGLLPAYHSSAHLYICHTGNEFISSA
                     QELAQRLRKHGLNVAVDLTTRKVGDQIKLADKQRIPFISVVGEEEIKDNKFKIKHLAS
                     GEEQKLVESEIIEFINSR"
     gene            3673..4266
                     /locus_tag="VI981_04955"
     CDS             3673..4266
                     /locus_tag="VI981_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEY4479672.1"
                     /translation="MVIHINVRKVVLDIETQNLFRDVGVNDPAALTISVVGIYDYQTD
                     AYTTYLESELSKLWLILEKTDILIGFNSNHFDIPLLNKYYPGDLTKIKSIDLLHEIKS
                     ASGKRIGLGYIAKATLNRGKIGHGLEAITWWKNGEVERVRKYCLEDVKLTKELYEYAR
                     QHGVLKYPDGSQIFDIKIDASKWEDKIESAMTYTLPF"
BASE COUNT         1290 a          972 c         1032 g         1260 t
ORIGIN      
        1 ctatatcggc gagacgattt gcgaaaatgc agactctata ccacttcctg caatatgtat
       61 tgacgaaccg acaattagct tgaattttct cgtaaatgat tcgccatttg ccgggagaga
      121 cggtaaatat gtaacgggca gacagcttcg cgagcgcttg gcgagagaac tggagacaaa
      181 tgtcgggtta aaggttgatt tttcaccgga aactaacaga gtcggaaaaa caccttattt
      241 taaagtctac ggacgcggag agcttcatat tgctattttg attgaaaata tgagacgtga
      301 gggttatgaa ttgcaggtat ctcaaccaca ggtaatcatc aaggaggacg ctggcgtcaa
      361 aatggagcct tttgaagaag tcattattga cgttaaggaa aagctttcga gctccgtaat
      421 tcaaacactt agcgagcgcc gcggccaact tcttgaaatg aaggaacacg gcggaaatgt
      481 tcggataatt tttgagatac cgacgagagg tcttctcggg tatcgaagcc aatttatcat
      541 tgacacaaag ggtgaaggca ttctctcttc aagatttacg gcttttaagg aatatatagg
      601 cccgatcgat cgacactctt ttggttcgat gatttccatg gcgtccggaa ttgcgcttgc
      661 gtattcactc tggaatttgc aggagcgcgg gcggatatat gtaaatccgg gcacgcctgt
      721 atacgaagga atgatcatcg gaaatgtcac taaaggggat aatcttgacg ttaatcctac
      781 aaaaaacaaa cagatgacga acgtgcgaaa ttccggaaac gatgacgcga ttttgcttga
      841 gacacccctg cttttgacta ttgaacgcgg catggagatc atggcagaag atgactttct
      901 tgaagtcact ccgaagaacg tgcgcctacg gaagaaatat cttagagaaa ctgatcgtac
      961 aagggccgcg cgcagtaaat aatctgaatc tctaatcact gacagccgga cctatgctgg
     1021 caattgtttg tttcaaaaac tggtcttcga caaaacaaaa cccgcgcgtc gcgcgggttt
     1081 tgttcaatct cctaaagata gagatattag aagcttcgtc ggtcacctcg atcgtaactg
     1141 cctccccttg aaggccggtc gcttcgtggt gccatagggc gagcctcatt tacggtaagg
     1201 gttcgtccgt cgagctcctt tccgtgccac atgtcgatag ctgcttgagc agcctcatca
     1261 gccattgtca cgaagccgaa gcctcgactt ttacccgtca tcttgtcggt aaggattgct
     1321 acatcttcca cagtgccggc ctttgagaat gcatctctaa gtccgtcatt gtttgtgttg
     1381 taggaaatac ctccaacgta caactttttt gacatttgaa actaatttct aacctactaa
     1441 ttctgtagac gacccttccc tatttctaaa aagaaacgag gaacattcga tgttcctata
     1501 aacggaaact ctacatacgc ggatcatacc ctacaatagc tattcaggca agggtacggg
     1561 taatttgagg ctctgtttaa gggtgtaatc tccaacgctc actcttttta tgtgaagcgc
     1621 aacggcagac gtatcgagca gaagagcgag cttctccacg agcgcacgaa cgtatgtccc
     1681 gcttgagcag gatactttaa gcgtatagat atgatgccga catgatccaa tacgcgcaaa
     1741 atttaacttc cagagttgcc gtatttcatc ctgtctaaaa tccccatgca ctttcgatat
     1801 tctgtcggca ataaaattcg caagcgctgc ggacgtcagc acatatgacc ctactatatc
     1861 caatgagaag atctcgactt gcctattagg aatatcaatt tcggttaacc tgtctccgcg
     1921 agcccattca aatagatgct tgcctcgtac agtcttagac gaataggcgg gatatgtctg
     1981 cgttgcggac cctatgagcg ccagtaatat acttttcagc gattcaggca gtatttcggt
     2041 tggcaaactc aacttcgtga ttttacctaa aatgtcatgg gtgtcagtgg cgaccccaaa
     2101 taggatgtcg aatttatact ctttcggtag tgataaatac tcttttttcc gctttatgct
     2161 ttcatcagcg agaattatca agactccttc ggcgagagga tcaagacgtc cggcgtaggt
     2221 gagcttcttg tttttaagct ctggatatgt cacacgaagt ctttgtatgc acgcaagcgg
     2281 cgtctctcct atctttttgt atacgttgta tacggtgtct ctcatatttt tatcgtatac
     2341 tacgatgttc atctttgcca tattgtcgga tatttgaatt gagaaatccc gagtattacc
     2401 gtaaaatgca ttaatggcta aagtcgcaac agaaccatac aagggcgtaa gagatttcta
     2461 tccggaagag tttgcggttg agaaatacat ctggaaaaaa tgggtacagg tggcggaaag
     2521 tttcggttat gttgagtaca atgcttcaat tcttgaaccc tctgaactct accgaggaaa
     2581 aacaagcgag gagatcgtta atgagcaaac ctacacattc actgaccgtg gcgatcgaga
     2641 agtcacattg cggccagaaa tgactccgac tgtggcgcgt atgattgcgg caaaacgccg
     2701 tgaactatcg tttccactca ggtggttttc tattcccaat gtattcaggt atgagagacc
     2761 ccagcgcggc cgtctacggg aacattggca gcttaatgcc gatattttcg gtgttcctgg
     2821 aattgaggca gaggtggaaa tcatctccct cgcctattac atcatgaaag ccttcggggc
     2881 aaaggataac gattttactc taaagattgg tagtcgagct tctctcaatg accttgctag
     2941 acgacaaaat ttaacagagg agcagttcaa aaaactaatt ggattactag accgccgaaa
     3001 taaaataact cccgcagaat ttcatactgg aatcagtgaa ttaggcttta cattggacat
     3061 actatctttg caagaccctc tcgcagacgt cccaagaatc tgtgacctac ttaaagataa
     3121 gggcataaaa aatgcaattt atgacccttc tatagtgcgc ggcttcgact actacacagg
     3181 aactgtcttt gaggtttttg atactaaccc aaaaaacaat cggtcacttt ttggtggcgg
     3241 acgatatgac aacttgcttg atctttttgg tgaagagccg gctccggctg tcggttttgg
     3301 catgggagac gttaccattc gtgactttct cgaaactcgc ggtcttttgc cggcgtacca
     3361 ctcttctgct catctttata tttgtcacac tggtaatgag tttatttcga gtgcgcaaga
     3421 actagcgcag cgtcttcgca aacatggcct caatgttgct gttgacctta caaccagaaa
     3481 agttggtgac cagattaaac tagcggataa gcagaggatt ccatttatca gcgttgtcgg
     3541 cgaggaggaa attaaagata acaagtttaa aattaagcat cttgctagtg gagaagaaca
     3601 gaagctcgta gaaagcgaga tcatcgaatt tattaatagt agatgaaaat gtgattccta
     3661 tattcggact aattggtaat tcatattaat gtgagaaaag tagttttaga tattgagacg
     3721 caaaatttat ttcgcgatgt cggagtgaat gatccggccg ctttaaccat ttcagtggtc
     3781 ggaatttacg actatcaaac tgacgcctat accacctacc ttgagtccga actgtccaaa
     3841 ctctggctta ttcttgaaaa aacggacatt ttaattggct ttaattccaa tcactttgat
     3901 atcccgcttt tgaataagta ttacccggga gatctcacaa aaatcaaaag catcgattta
     3961 ctgcacgaaa taaagagtgc ctcgggtaaa cgtatcggac tcggttatat cgccaaggct
     4021 acgttgaatc gcggcaagat tggacatggt cttgaggcaa ttacgtggtg gaaaaacggt
     4081 gaggttgagc gcgtcagaaa atactgtctt gaggatgtaa aactgacaaa agaactttat
     4141 gaatatgcaa ggcaacacgg agtattaaaa taccccgacg gttctcaaat ttttgacatc
     4201 aagatagatg cctcaaaatg ggaagataaa atagaaagcg cgatgacgta tacactgccg
     4261 ttttgactag cttttagctt ttagcttata gctgttagct tttaggagaa catcgtcggc
     4321 accctattac aaagatcgac attgatgccg aggttaaacc ttctgggaat ttccagtttt
     4381 cattaaaact gcaaaattga tcattaccga gcaagcctag ctcgtgtaag tggtcctacg
     4441 gtaccagtac tctctattcc gttcttgctc tgatactgct tcactgcttg tttggtaaga
     4501 ggaccataga agccagtgct ggagtcagcg ggcagtaggc cttctttaat gagg
//
LOCUS       DATDSU010000142         4042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1144054, whole genome shotgun sequence.
ACCESSION   DATDSU010000142 DATDSU010000000
VERSION     DATDSU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144054"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VI981_04960"
     CDS             <1..886
                     /locus_tag="VI981_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4479673.1"
                     /translation="FALSYFPIQTKKEGAKAEATAPSPRADEPAAEADSFKDIDITAR
                     AAYVWDITNQKALYNKNAGKQLPLASLTKLMTALIAYEHLDAEDRVAITTEALLEEGD
                     SMFSGGEIFTMHDLADFTLITSSNDGAYALAATAGTALASSQNPTGAFVAAMNAKAEE
                     LGLSQSYFTNPTGLDVDGTQSGSYGSARDMAFLMEYLALAHPEILAGTKAAHRIIYNQ
                     EGAALQAVNTNERIADIPGLIGSKTGFTRLAGGNLVIAYDAGLNRPIVIAVLGSTRKG
                     RFSDVTTLINATAHVLTQ"
     gene            900..2474
                     /locus_tag="VI981_04965"
     CDS             900..2474
                     /locus_tag="VI981_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY4479674.1"
                     /translation="MSLATISEASKWATNYLEKEVSPTNISYLVQYGKVKKHGENGST
                     MVDLIDLKKYYASYNGKREISWKKNLGNDLNWILSFDNLREKDTTKHVHRLHPYKGKY
                     IPQLVEYFIDTHTDDFKKEAYFKTGDIILDPFLGSGTTLIQSLEMGIHSVGIDVSEFN
                     CMIASCKATHYDDEYLQKAIKKMLASLDTFEHDNKIQEFESELLVELAKFNNIHFPGY
                     DFKYKINQGNFDEKKFSIEKERKFLPVYQKLLKKYSIKLKQDKSESFLDIWFMDNVRR
                     EIDHVFQTIKQEKDIKTRKILALILSRTIRSCRATTHSDLATLKEPQLTTYYCYKHKK
                     ICKPLFSIKTMLNRYALDTLSRIREFNRLRNPVHYSVLTGDSRTIDIFEKVEKQNLEF
                     SKILRRQKIAGIFCSPPYVGQIDYHEQHAYAYDLLGFKRKDDLEIGPLYKGQGLEARR
                     SYAQGIADVLNNCKQYLKTDFDIFLVANDKYNLYPEIAEKSGMKIVNQFKRPVLNRTE
                     RDRNPYSEIIFHFKLK"
     gene            2478..3263
                     /locus_tag="VI981_04970"
     CDS             2478..3263
                     /locus_tag="VI981_04970"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HEY4479675.1"
                     /translation="MALSKEHINSVGEVLKASLRNKFQNYKPEPASMPFHTRLLGKDR
                     LALYSFIHSLNTNFGTSIFEPVGLALAQKNFKMAVSQATAGNQMSSAAQAEIQKIIDG
                     LITATSTPNKKEEIERIRKVCQTGEMIKVKPTKIDLMFESKDGAYYLFDIKTAKPNAG
                     GFKEFKRTLLEWVAVVLANKPDAVINTFIAIPYNPYEPEPYTRWTMRGMLDLENELKV
                     ADEFWDFLGGKDTYKDLLDCFERVGIELRGEIDTYFKRFKESP"
     gene            3260..>4042
                     /locus_tag="VI981_04975"
     CDS             3260..>4042
                     /locus_tag="VI981_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479676.1"
                     /translation="MTDIIKIFAPAVLAFAVGIAMTPMLTHYLFHFKVWKKTPGKYAL
                     DGTAATEFNKLHKENEAHTPRMGGIVIWGSVFVTTLACWAIAFFFPSSPLAGLDFVSR
                     SQTWIPFSTLLLGACVGFLNDLYDVQEGKRGIRLRTRLIFVSLLSLAVGWWFYEKLDV
                     ISIGLPYDGELVLGALIIPFFMLMTLALYASGVIDGIDGLSGGVFASIFTAYSVIAFS
                     QQQFDLAAFCATVAGAILAFLWFNIPPARFWMTETGTMALTLT"
BASE COUNT         1188 a          940 c          893 g         1021 t
ORIGIN      
        1 ctttgcgctt tcgtacttcc cgatacaaac aaagaaagaa ggtgcaaaag cggaggcaac
       61 cgcaccaagc ccgcgcgcag acgagcccgc cgccgaagcc gattctttca aagacattga
      121 catcaccgct cgcgccgcgt atgtgtggga catcacaaac cagaaagcac tgtacaacaa
      181 aaatgcgggc aaacaactgc cgcttgcgtc tctgacgaag cttatgaccg cgcttatcgc
      241 atatgagcac cttgatgcgg aggaccgcgt cgccatcaca acggaggcgc tccttgagga
      301 gggagacagc atgttttccg gcggcgagat atttaccatg cacgacctcg ccgacttcac
      361 gctcatcacg tcctccaatg acggagcata cgcgctcgcg gcgacggcag gcaccgcgct
      421 tgcttcaagc caaaatccga ccggcgcgtt cgttgccgcc atgaacgcaa aggcggaaga
      481 gctcgggctt tcgcaatcgt attttaccaa tccgaccggt cttgatgtcg atggcacaca
      541 gagcggcagt tacggctcgg cgcgcgatat ggcgtttttg atggagtacc ttgcgctcgc
      601 gcatccggaa atccttgcgg gaacaaaagc cgcgcaccgt atcatctaca accaagaggg
      661 cgcggcattg caggcagtaa acaccaacga gcgcattgcc gacatccccg gccttatcgg
      721 ctcaaaaacc ggctttactc ggctcgccgg cggcaacctc gtcatcgcct acgatgccgg
      781 gctcaacaga cccatcgtca ttgcggtgct cggctcgacc cgtaaaggac gcttcagcga
      841 tgtcacaacg cttatcaacg caaccgccca cgtgctcacc cagtaaataa acacactgta
      901 tgtctttggc tactatttcg gaagcatcaa aatgggctac caactatctt gaaaaagagg
      961 tgtctccgac aaacatttcc taccttgtac aatacggcaa ggtaaaaaaa catggtgaaa
     1021 atggttctac catggttgat ttaatcgacc tcaaaaaata ttacgcctcc tataatggga
     1081 aacgcgaaat cagttggaag aaaaatctcg gtaatgattt aaactggata ctttcctttg
     1141 ataatttacg cgagaaagat accacaaagc atgtacaccg gcttcatccg tacaaaggga
     1201 aatatattcc acaacttgtt gagtatttta tagacacaca tacggacgat ttcaagaaag
     1261 aagcatattt taaaacgggc gatattattc ttgacccatt tttgggttca ggaacaacac
     1321 ttatccaatc gttagagatg ggtattcatt cggtcggcat agatgtttca gagtttaatt
     1381 gcatgattgc gagttgtaag gcaacacatt atgacgatga gtatttacag aaagcgatca
     1441 aaaaaatgct cgcttctctt gatacatttg agcacgacaa taaaattcaa gaatttgaaa
     1501 gcgagcttct agttgagctt gctaagttca acaacataca ttttcccggt tacgatttta
     1561 agtacaaaat aaatcaagga aattttgacg aaaagaaatt ttctatcgaa aaagagcgca
     1621 agtttttgcc tgtttatcaa aaattgctga aaaaatactc aattaaacta aaacaggaca
     1681 aatcagaatc tttccttgat atttggttta tggataatgt ccgcagagag atagaccatg
     1741 tctttcagac aattaaacaa gaaaaagata ttaagacccg caaaatcctc gcgcttattt
     1801 taagtcgaac aattaggtcg tgccgtgcaa caacacatag cgacctggca accctaaaag
     1861 agcctcaact tacaacctat tactgctaca agcacaagaa aatttgtaaa ccgcttttct
     1921 ctatcaaaac aatgctcaat cgctacgctc tcgatacctt gagcagaatc agagaattta
     1981 atagactacg caatcctgtc cactattctg tcttaaccgg agactcccga accatagata
     2041 tttttgagaa agtagaaaaa cagaatctgg agttttctaa aattcttaga aggcaaaaaa
     2101 tagcgggtat tttttgctca cctccgtatg tcggacagat tgattatcat gagcaacacg
     2161 cctatgctta cgatttactt ggatttaaga ggaaggatga tttggaaata ggtcctctgt
     2221 acaaaggaca aggactagag gcgcgccggt catacgcgca aggcattgca gatgtattaa
     2281 ataactgtaa gcaataccta aaaactgatt ttgatatttt tcttgtggcg aatgataaat
     2341 acaatcttta ccccgaaatt gcagaaaaat cgggaatgaa aatagtaaat cagtttaaac
     2401 gacccgtatt gaaccgaaca gagcgagatc gtaaccctta ttcagaaata atttttcatt
     2461 ttaaattaaa ataatctatg gctttatcta aagaacacat aaatagtgtc ggagaagttt
     2521 tgaaagcaag tttgcgtaat aagtttcaaa actataaacc agaaccggca tcaatgcctt
     2581 ttcatacacg gctactcggc aaggatagac ttgctttgta ttcgtttatt cactctctca
     2641 atacaaattt tggtacgagt atttttgaac cggttggtct tgccttggcg caaaagaatt
     2701 tcaaaatggc tgtttcgcaa gctacggccg gaaatcaaat gagttctgcc gcgcaagcag
     2761 aaatccagaa gataattgac ggactcatta cggcaacaag tacaccgaat aagaaagaag
     2821 aaattgagag aatacgaaaa gtgtgtcaaa caggtgagat gattaaagta aagccgacca
     2881 aaatcgattt aatgtttgag tcaaaagatg gtgcgtatta cttgtttgat attaaaacag
     2941 caaaaccaaa tgctggagga tttaaagagt ttaagcgcac cttacttgag tgggttgctg
     3001 ttgttttggc aaacaaacct gatgcagtga taaatacctt tatagcaatt ccatacaatc
     3061 cttacgaacc agaaccatat acccgttgga caatgcgagg aatgttagat ttggaaaatg
     3121 aacttaaagt agcagatgag ttttgggatt ttctcggtgg taaagacaca tacaaagatt
     3181 tgctcgactg ctttgagaga gtgggtattg aattaagagg tgaaattgat acctacttta
     3241 agcgctttaa agaatcacca tgactgacat cataaaaatc tttgcgccgg cggtactcgc
     3301 atttgcggtt ggcatcgcca tgacgccgat gttgacgcac tatttgtttc atttcaaggt
     3361 atggaaaaaa acgccgggca aatatgcgct cgacggcacc gccgcgaccg agtttaacaa
     3421 gctccacaaa gagaacgaag cgcacacgcc gcggatgggc ggtatcgtca tctggggcag
     3481 tgttttcgta acgacgctcg catgctgggc aattgcattt ttctttcctt cctcgccgct
     3541 tgccggactc gacttcgtca gccgttcgca gacatggatt ccgttttcca cgctcctcct
     3601 cggcgcatgt gtcggcttcc tcaatgacct ctacgatgta caggagggga agcgggggat
     3661 acgcctgcgt acgcgactta ttttcgtatc gctcctgtca ctcgccgtcg gctggtggtt
     3721 ctatgagaaa ctcgatgtca tcagcatcgg tttgccgtac gacggcgagc tcgtactcgg
     3781 cgcactcatc attccttttt tcatgctcat gacactcgcg ctctacgcaa gcggcgtcat
     3841 cgacggcatc gacggcttaa gcggcggagt gttcgcttcc atttttaccg cctattccgt
     3901 tattgcgttt tcccaacaac agttcgacct cgcggcgttt tgcgcgaccg tcgcgggcgc
     3961 gatactcgca tttttgtggt ttaacattcc gcccgcgcgc ttttggatga cggagaccgg
     4021 caccatggcg ctcacgctca cg
//
LOCUS       DATDSU010000143         7246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1329309, whole genome shotgun sequence.
ACCESSION   DATDSU010000143 DATDSU010000000
VERSION     DATDSU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329309"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..612
                     /gene="tnpA"
                     /locus_tag="VI981_04980"
     CDS             340..612
                     /gene="tnpA"
                     /locus_tag="VI981_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEY4479677.1"
                     /translation="MKTFGFHVLWPHDVSFHPKYSIGQFVRLYKSITAKQIFLKFPEI
                     KKELWGDEFWTDGYYAATVGEQGNWHIAEQYIKKQGKHDVGTLILW"
     gene            682..1509
                     /locus_tag="VI981_04985"
     CDS             682..1509
                     /locus_tag="VI981_04985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479678.1"
                     /translation="MNPLYISVVTPTLNRAKLLEKNLKSILDQEYPYIEHIVVDGISK
                     DGTVELLMAYELKYAAKGCKLRWISEKDKNHTSAVNKGFKLATGDYVGFMYSDEYWAS
                     GAIRKLVEEIQKDPPLDILYGDVFQYYEDSKEIKRLLFRQYSLKDLIYSGEQIPLAGA
                     VVRRAFFDKVGYLKENLEFVAEHDFLVRCIEYGAKSYYVHFPIAYIFEHSMRKVLLDI
                     PASWKETKKVNFAHGAHYLTPFYMNYVANMYLRPVFDVMKSRAPGLYNALKKVKDSL"
     gene            1549..2496
                     /locus_tag="VI981_04990"
     CDS             1549..2496
                     /locus_tag="VI981_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4479679.1"
                     /translation="MNKDAKVLVVGGAGYVGGSVTDELIKQKLSFTVYDNLLYEERYL
                     KPVDFIYGDVRDTEKLSKILPDYTHVIWLAAIVGDGACALHPEATIEINELAVKWLAE
                     HFAGRIIFTSTCSVYGHNEKEVTEEDETAPMSLYAITKINAERYLSVKNVLILRLGTA
                     FGISDAFSRPRSDLLVNTLVSNAVVKGKVMVFGGSQWRPNIHVQDIAEIIVEGLRSSL
                     KGVYNVATQNFTVDEIAKKISVLTRCDIQYAGQLREDRRNYRVSFAKALTSGLLKLPT
                     EHDIEYGASEFVRLIESGRIKDVKGSVYSNAKHLSNYSI"
     gene            2493..2954
                     /locus_tag="VI981_04995"
     CDS             2493..2954
                     /locus_tag="VI981_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY4479680.1"
                     /translation="MKNDSRTPFSMNVDVFSDDRGAFVPFFDLEKNGEITKAMGSVKR
                     VYYVHNPTRGTIRGFHYHKKEWKFFVIVSGSAKFVALNPDDPKELYTFVSSERKSNAI
                     VVPPGYANGWASLEDNTILVCASNATTSESRADDKRFDPMKWGDVWKVSPR"
     gene            2933..3856
                     /locus_tag="VI981_05000"
     CDS             2933..3856
                     /locus_tag="VI981_05000"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEY4479681.1"
                     /translation="MEGESEMIYAHNPMQKKSVAILGATGMLGNALYAGLKDAYELIL
                     VVRNTKKVPLLERKYGYTAEHQIIEFDAAKSYEDFLSNKGYPDGHLLRLFDSLKDADY
                     VINAIGVTIPFSSENPALTFFINGVLPHLLAAHFGEHLIHITTDCVYDGKTGFPYDEN
                     SPKSPPDLYGLSKSLGEPTNCLTLRTSIVGRELEGFTGLLEWFLTQKGKAVKGFANHL
                     WNGITTKEFAKICGKIMSDPSKYHKIGLYHVFSTTVSKYEMLLVFQKKFGIDCKIVPE
                     YENKLNRTLSTVYKFNNLFKILSFEEMIQEL"
     gene            3870..4145
                     /locus_tag="VI981_05005"
     CDS             3870..4145
                     /locus_tag="VI981_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479682.1"
                     /translation="MAKEFSQSDFETEKQKERERIHDLIQEFRDVLAKNQYEIPAGTE
                     VVMVLSAPPDLEFKDNPEQREKTPENISRISFAHDIYKQLAAKKLNT"
     gene            4253..5563
                     /locus_tag="VI981_05010"
     CDS             4253..5563
                     /locus_tag="VI981_05010"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEY4479683.1"
                     /translation="MPRTGSKNVKVAFTAGRLTHFGGVFLLHQFLHTIKLRTYLHERL
                     RMVERNNHFSITERLVALLYPTILGLDKIELTALLGTNGVFQYLTGLPRVPNPTTLRR
                     FLITNAHVLLPKLRTAHNLLRAHFLDLLESRSSYWLDFDSTARTLYGNQEGALKGYNP
                     HHPGKKSYHPLICTEARLQDCLGGELRYGNVHTATGVDAMLNAVLEILPASTRIIRTR
                     ADAGFYDGDFIQQLCDNRIEFVVVAHMTVPLKHKIPALRYTKIDGVYSSAEFQYQPHG
                     WKQKHRFVVLREQLTDKRDAQLTLFKMKAYAYHVVVTNLSLTPQGVFTFYKNRSAMER
                     TVRTLKEDYQFGAAPTGNFAANALYAELSLLAYNIVIWFRRLCLPNDWQSYTVATLRH
                     RLLLVPGVFTKTNNRPTLKLPKNNPHQNTFLFAEERIKKMKSLI"
     gene            5742..6086
                     /locus_tag="VI981_05015"
     CDS             5742..6086
                     /locus_tag="VI981_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HEY4479684.1"
                     /translation="MKEIAVAIGIPEEKLYLLSCGNRGAGNTRTQFTQIMNSPDLKSR
                     SHFIVVTSAYHVSRVARTAKKHLPEFVDFEVFGVPFKDFSFSVFRKVKGEVKRILSYS
                     RKGDIAEDVRRK"
     gene            complement(6090..6965)
                     /locus_tag="VI981_05020"
     CDS             complement(6090..6965)
                     /locus_tag="VI981_05020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479685.1"
                     /translation="MSKNICAIIVTWNADKDDLERTIPAASHEADSVVVVDNGSLPEN
                     FAALKKITEKFRNVTIIQNTENKGIGNAFNRGAEYALKKGFEWFITLDDNGKMGERML
                     GKLLEAYDNLAEKEQAKTAIVAPNYTTLKGRVYEAENPFFVQTTVATGQMIKTKAWKE
                     VKGFKEDLFIACVDHEFCFKILRAGYKTLLVPSTMLYATAGPKPIVRSLFGKKFVVPN
                     YSPDRYYYTYRNSMYLYKMYWRHVPGWIIKNIISNKLTFLKILFFEEQKFKKILMIIQ
                     GCWDGLRNKLGKLKQ"
     gene            complement(6985..>7246)
                     /locus_tag="VI981_05025"
     CDS             complement(6985..>7246)
                     /locus_tag="VI981_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479686.1"
                     /translation="IVAELVTNGFIWREMQKVNQFTVLRRISRILAAGCIMAASAIIM
                     KHVDMPFVPNVLVSAAIYGILLVLFREPLIKIFKNRPEERPA"
BASE COUNT         2023 a         1497 c         1668 g         2058 t
ORIGIN      
        1 gatggccgag agcgttgctc cctgcgcgag gcctcggctg atttcctcgc gctcggcgga
       61 tgaaagccgc cggtattgta ttgggttgtg catacggata tattgtactc atatccgttg
      121 cgctaaatgt ctgactccaa gtacttaata gccagtctaa ttaaaattat tctaaaacag
      181 aatttattat aagacaagga gtagtctttt ccagcgcaac atgataatga attgttgctt
      241 gcaattgtgt cggcatttgt tatgatattc tcgtgcgcgc ttaataaata tcttactcat
      301 ttccctgcgg tctctgccgc agggaccccg taggaaagta tgaaaacctt cggttttcat
      361 gtactatggc ctcacgacgt ctcattccac ccaaagtatt caattggaca attcgtgcgc
      421 ttgtacaaaa gcattactgc aaaacaaatc tttttgaaat ttcccgagat caagaaagag
      481 ctctggggtg atgaattctg gaccgacggc tactatgctg ccacggtcgg agaacaagga
      541 aactggcaca tcgccgagca atatatcaaa aagcagggga aacacgatgt gggaacgctc
      601 atcttatggt aaactcaaga atataccaag cgtcatgagt cctcagcata ccctgcggtc
      661 tctgtcgcgg ggttgttgat tatgaatcct ttatatatta gtgtagtgac tccgacgctc
      721 aatcgagcta aattgctcga aaagaactta aaaagcatat tggatcaaga atatccttat
      781 atagaacaca tagtggttga tggtatatca aaggatggaa ccgtagagtt gctaatggcg
      841 tatgaactga aatatgcagc gaaaggatgt aaattgcgct ggatttctga aaaggataaa
      901 aatcatacat cggccgtaaa caagggattt aaattagcaa ctggtgatta tgtcggcttt
      961 atgtactctg atgaatattg ggcttctgga gcgattcgga agctggtaga agaaatacaa
     1021 aaagatccgc cacttgatat tttatacgga gacgtttttc aatattacga agatagtaag
     1081 gagataaaac gtttattatt tcgccaatac tcacttaaag atttgattta tagtggagaa
     1141 caaattccgc tcgcaggagc cgttgtccgc agagcatttt ttgataaagt gggctatctt
     1201 aaagaaaatc ttgaatttgt tgccgagcat gatttcctcg tgcgctgtat agagtatggg
     1261 gcaaaaagct attatgtcca ctttccgatt gcgtatatat ttgaacattc aatgcggaaa
     1321 gttctccttg atattccggc atcatggaaa gagacgaaaa aagtaaattt tgcccatggc
     1381 gcgcattatc ttacgccgtt ctatatgaat tatgttgcca acatgtattt gcgtcctgtg
     1441 tttgatgtaa tgaagagcag ggctcctggt ttgtacaatg cattaaaaaa agtaaaggat
     1501 agtttgtaaa agttgacaca aacacattgt gtcgattagc atggtgatat gaataaagat
     1561 gcgaaggttc ttgtggtggg gggtgccggc tatgttggcg gctccgtaac cgacgaatta
     1621 ataaaacaaa aactctcttt tactgtatac gataatttgc tgtatgaaga gcggtattta
     1681 aaacccgttg attttatata tggtgatgtg cgagataccg aaaaactttc aaaaatactt
     1741 cctgactata ctcacgtcat atggcttgcg gcaattgtgg gtgatggtgc atgtgcgctt
     1801 catccggagg cgacaataga gattaacgaa cttgctgtca agtggcttgc agaacatttt
     1861 gctgggagga tcatttttac ttctacgtgc tctgtatatg gccataacga aaaggaagtt
     1921 actgaagaag atgagacggc tcccatgtcg ctttatgcga ttacaaaaat aaatgccgaa
     1981 cgttatcttt ctgtgaagaa cgtccttatc cttcgtcttg gcaccgcctt tggtatatcg
     2041 gatgcgttca gccgtcctcg atcagatcta ttggtgaaca ctcttgtaag caatgcagtt
     2101 gtcaagggaa aagtaatggt ttttggcgga agtcagtgga ggccgaatat tcatgttcaa
     2161 gacattgccg agataattgt agagggattg agatcgtcac tcaaaggggt gtataatgtg
     2221 gcaacgcaga attttaccgt ggatgaaatt gcgaaaaaga tctcggtgct tacccggtgc
     2281 gatatacagt atgctggtca gctccgagaa gaccgtagga attaccgggt aagttttgca
     2341 aaagccttga ccagcggcct ccttaaatta cccaccgagc atgatatcga atacggagcg
     2401 agtgagtttg tccggctcat agaaagtggt agaattaaag atgtgaaggg aagtgtctac
     2461 tcgaacgcta aacacctttc gaattactcc atatgaagaa cgattcccga accccgtttt
     2521 ctatgaatgt cgatgttttt tcagacgatc gcggcgcttt cgtgcctttt ttcgatttgg
     2581 aaaaaaacgg cgagattacg aaagcgatgg ggtcagtgaa acgggtctat tatgtgcata
     2641 atcccacgcg cggtacgatt cgcgggtttc attatcataa aaaggaatgg aagttttttg
     2701 tgattgtttc tggaagcgca aaattcgtcg ctcttaatcc tgacgatccg aaagagttgt
     2761 atacttttgt aagctccgag aggaaaagta acgcgattgt cgttcctccg ggatatgcga
     2821 atggttgggc ttcgcttgaa gacaatacga tattggtatg cgcctcaaat gcgacaactt
     2881 cagaatcacg ggcggatgac aagcggtttg atccaatgaa atggggggat gtgtggaagg
     2941 tgagtccgag atgatctacg cccataatcc gatgcagaaa aaatctgttg caattttggg
     3001 tgcgaccggt atgttgggga atgcgctcta cgcaggcctc aaggatgcat acgagctcat
     3061 actggttgtg cgtaatacaa aaaaagttcc gcttcttgaa cggaagtacg ggtacactgc
     3121 ggagcatcag attatagaat tcgacgccgc gaaaagctac gaggattttt tgagtaataa
     3181 gggatatccg gacgggcact tgcttcgcct ttttgattcc ttaaaagacg ccgattacgt
     3241 tataaatgcg atcggcgtaa cgattccgtt ttcttcggag aatcccgcgc ttacattttt
     3301 tataaacggc gtccttccgc atcttttggc ggcgcacttc ggcgagcacc ttattcatat
     3361 tacgaccgat tgtgtatatg acgggaaaac aggatttccg tatgatgaaa attctccgaa
     3421 atcgcctccc gatctttacg gtttgtcaaa aagcttggga gagccgacga attgtttgac
     3481 gcttcgaact tccatcgtgg gccgggagct ggagggtttt accggacttc tcgaatggtt
     3541 tttaacgcag aaagggaagg cggtaaaagg atttgcaaat catctttgga atggcatcac
     3601 cacaaaggaa tttgcaaaaa tatgcggcaa gataatgtcg gacccctcga aatatcataa
     3661 aataggcctt tatcacgtat tttcaactac ggtttcgaaa tacgagatgc ttctcgtttt
     3721 tcagaaaaaa ttcggcattg attgtaaaat tgtgcctgaa tacgaaaata aattaaaccg
     3781 caccctaagc acggtttaca aattcaacaa tttatttaag attctttcat ttgaggaaat
     3841 gatccaagaa ctttaatccg tatattatta tggcaaaaga attttcacaa agtgattttg
     3901 aaacagaaaa acaaaaagaa cgcgagcgga ttcatgattt aattcaagag ttccgtgatg
     3961 tactggcgaa gaatcaatat gagattcccg caggaaccga ggtggtaatg gttttgtctg
     4021 ccccgcccga ccttgaattt aaagacaatc ccgaacagcg ggagaaaacg ccggagaata
     4081 tttctcgaat ttcgtttgct cacgatatat acaagcagct tgcggcaaag aaattaaata
     4141 cttaaatcct gcgtgaaaat agatagaatt tcttaaaagc tctttcgagt gcacacatta
     4201 tcgatatgtg ctatactcct tttgaaccta acttgtagtt agcttcaaaa gtatgcccag
     4261 aactggttca aaaaatgtta aagtcgcgtt tactgccggt cggctgacac atttcggcgg
     4321 cgtatttctt ctccaccagt ttcttcacac tatcaaactc agaacatact tgcatgaaag
     4381 attacgcatg gtggaacgca acaaccattt cagcatcact gaacgtcttg tcgccctcct
     4441 gtaccccacg attctcggac tcgataagat tgaattgact gctttgctag gaacaaacgg
     4501 ggtattccaa tatctcaccg gacttccgcg cgtaccaaac ccaaccacac tcaggagatt
     4561 tttgattacc aacgcacacg ttcttcttcc aaaactccgt accgcccaca atctcctgcg
     4621 cgcccatttt cttgatttac tggaatcgcg ttccagctat tggcttgatt tcgattcaac
     4681 agcccgaacc ctctacggaa atcaagaggg cgcgttgaaa gggtacaatc cccatcatcc
     4741 cggcaagaaa tcctaccatc cgctcatctg taccgaagct cgtctccaag attgtcttgg
     4801 gggtgaactt cgatacggaa acgtacacac ggcaacagga gttgatgcta tgctgaatgc
     4861 tgttttggaa atacttcctg cctcgacgcg aatcattcgt accagagctg acgcggggtt
     4921 ctatgatggc gattttatcc agcaattatg cgataatcgg attgaattcg tagtggtggc
     4981 gcatatgacc gtccccttga aacacaagat tcccgctttg cgatatacga agattgatgg
     5041 ggtgtattct tctgccgagt ttcagtatca accgcatggc tggaagcaaa aacatcggtt
     5101 tgtcgttcta cgcgagcagc tgacggacaa acgagatgcg cagctgacat tgtttaagat
     5161 gaaagcatat gcgtatcacg tcgttgtcac caatctttct ttgactccac agggcgtttt
     5221 caccttctat aaaaatcgtt cagccatgga acgaaccgtc cgtacgctta aggaggacta
     5281 tcaattcgga gccgcgccta caggaaattt tgcggccaat gcgttgtatg cggaattgtc
     5341 gctccttgcc tacaacatcg ttatctggtt tcggcggctt tgtctcccga atgactggca
     5401 atcatacacg gtagcgacac ttcgacatcg cctcctgctc gttcccggcg tcttcacaaa
     5461 gactaacaac cgaccgacgc ttaaattacc aaaaaacaat ccgcatcaga acacatttct
     5521 tttcgcggaa gaacgtatca aaaaaatgaa atcacttata tgaacggggc attatgcgca
     5581 cgacattctg aaaattgatt ggatttctaa ggcgtgaaat ttggtcattt tcacgcagga
     5641 tctaagtaaa taagcatgtt gaggaggtgc gcaatgagga tttgagcagc ttcgcgattc
     5701 cactttttat tttaaacggc gaagcggaac aattgcagcc aatgaaagaa attgcggttg
     5761 cgattggtat tccggaagaa aaattgtatc tattaagttg cggaaacagg ggcgctggca
     5821 atacaaggac gcaattcact caaataatga atagcccaga tctaaaaagc cgctctcatt
     5881 ttatcgtggt gacgtccgcg tatcacgttt cgagggtggc gcgtaccgca aaaaagcatt
     5941 tgcccgaatt tgtggatttc gaagttttcg gagttccgtt taaagatttt tcttttagcg
     6001 tattccgtaa agtaaaagga gaggttaagc gaattctttc ctattccagg aagggcgaca
     6061 tagcggaaga tgttcgtaga aaatagattc tattgtttta gttttccgag tttgttccgc
     6121 aatccatccc agcatccttg tatgatcatg aggatttttt tgaacttttg ttcctcaaag
     6181 aagaggattt ttaagaacgt aagcttattg gaaatgatat ttttgataat ccagccggga
     6241 acatggcgcc agtacatttt ataaagatac atactgtttc gatatgtgta gtagtagcgg
     6301 tcgggcgagt agtttggtac aacgaatttt tttccaaaca gggaacggac aatcggtttc
     6361 ggaccggcgg ttgcgtacag catggtactt ggaacaagga gggttttata tccggcgcgc
     6421 agaatcttaa aacagaactc atgatccacg caggcgataa agagatcctc tttgaatcct
     6481 ttgacttctt tccacgcctt tgtttttatc atttggcccg ttgcaaccgt cgtttgaaca
     6541 aaaaatggat tctcggcctc atatactctg ccctttaaag tagtatagtt gggcgcaacg
     6601 attgcggttt ttgcctgttc tttttcggcg agattgtcgt atgcttcaag caattttcca
     6661 agcattctct ctcccatttt tccgttatca tcgagcgtga tgaaccactc gaagcccttt
     6721 ttgagagcat attccgcccc gcggttaaat gcgttcccga tgcccttatt ttcagtattt
     6781 tgaataatgg tgacattcct gaatttctct gtgatttttt tgagggcggc aaaattttcc
     6841 ggcaacgatc cgttgtcaac gacaacaaca gaatccgcct cgtgagatgc tgcaggaatt
     6901 gtccgctcaa ggtcatcctt atcagcattc catgttacga taatcgcgca gatatttttt
     6961 gacatgtttt tttgcaggcg gccgttatgc tgggcgttct tccggccgat ttttaaaaat
     7021 ttttataagc ggttctcgaa aaagcacaag aagaattccg taaatcgcgg ccgatactaa
     7081 aacgttggga acgaatggca tgtcgacgtg tttcataatg atcgcgcttg cggccattat
     7141 acatccggcg gcgagtatgc gggatatgcg gcgcaggacg gtgaattggt ttactttttg
     7201 catttctctc cagatgaatc cgttagtaac taattcggcg acgatg
//
LOCUS       DATDSU010000144         7348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_480949, whole genome shotgun sequence.
ACCESSION   DATDSU010000144 DATDSU010000000
VERSION     DATDSU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480949"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VI981_05030"
     CDS             <1..611
                     /locus_tag="VI981_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEY4479687.1"
                     /translation="FVVILAGVLELVIFSLAGLPTNLLGQVTQLDWRILSLGAIIIGI
                     VEEVVKYAVLRATVFYSHDFNQIFDGIVYGITVALGFSIAENILYFLEFSEYSTGNFI
                     FAALFRALFSTLLHVMTAGIMGYYLGKAKFSLNNKKMYIVTGLVSAILIHAIFDFVIF
                     SSVTIVTLCTFIALIGAFGVFTRLWTHPEVRMIWRFDLQQGK"
     gene            676..1227
                     /locus_tag="VI981_05035"
     CDS             676..1227
                     /locus_tag="VI981_05035"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY4479688.1"
                     /translation="MLYTRKGDKGKTTLYGCNQSISKSSAIAEALGALDEINSFLGFC
                     KVRSAKAKLTVGKQKCSEIIHNLQETLFIIQAEVAGFPMNVDGKKVKDIEATIAAIEK
                     LLPPIKTFFISGGSELAASFDYARTIARRAERRVVGVSEEDKIKIGEQTLAYLNRLSS
                     ILYAFARLANIDAKKNEKPPSYK"
     gene            1400..1783
                     /locus_tag="VI981_05040"
     CDS             1400..1783
                     /locus_tag="VI981_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEY4479689.1"
                     /translation="MKAYLAIKYHKDGRNHGLIREICEVLRALEIETIAPVIHLETDG
                     KKLEPKELMQKSLAELRTCDILIAECSEKSVGLGIEAGYAFSIAKPIILIAKGGSEVS
                     PTLLGIAEKIIFYNNPAELSRKIRW"
     gene            complement(1786..3144)
                     /locus_tag="VI981_05045"
     CDS             complement(1786..3144)
                     /locus_tag="VI981_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4479690.1"
                     /translation="MNALGNSLKAGFARLRENPQLAYSILVALIIAFSFIFVSNRFIG
                     IAKSAEDNLINVRVGSIHDAFAAYVRLHSPYSTDIETMFRNVANDNTTISQFKLIGIE
                     NGKETLYASLDLSAEKGTPSEIAPEDAFLYNLAKSDPSRSFTTERYENGERFFKTARA
                     IADVSGEPKMLVTVQSLSEADKKIASEIRASIFILFLILFAVLLLFFRHSRIIDYGVL
                     YKRLREIDTLKDDFIAMASHELRTPLAAIKGYAELLLDRESEENDKQIFSKNILTSVR
                     NLDSLVADILDVARIEQGRIEVKLANCKTGELVSDVVDSLKKVASDKGLSLAVEIQDD
                     LLALVDRDRLRQVFVNLIGNAIKYTQKGNIKVTQKRENNRVVICVADTGIGMTKQEQG
                     KIFEKFYRVKSEETKEIGGTGLGLWITRQLVLKMSGSIYIESIKGVGTHAYVSFPIVH
                     SQ"
     gene            complement(3141..4646)
                     /locus_tag="VI981_05050"
     CDS             complement(3141..4646)
                     /locus_tag="VI981_05050"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY4479691.1"
                     /translation="MQKKITFFKKILFALSFVFYFLLLANTASSYENGALFRTDTNSV
                     STSTSDGSSQIQSTSDSSSGDGSLVSPLASPPSTTDTDTSQTSTEDKTTASSTLNVGP
                     SSTTEDGAIEDLRDRNTSTLEIQTETNKSLDAIASEIDQTLQTTLASSTVSDSTRTEI
                     NASVNEVKENLINDVRIDIERALEDTSTLRTPEIDIKKIEQNVETSFKEIERIVKEKT
                     NGTISTEQNKKDIVAYVEKLNDVIKKYAVEGRQKDSLSAYRDEDRDGISDFDERYIYG
                     TDPKNATTTPGELTDAEKVLKGIDPLSTTSALMIYDDPHERGKVAPDLFSIKNIEIVD
                     LSETDHKGKIQMSGRALPNSFVTIFIYSTPVIVTVKTNFSGEWTYTLEKELADGSHEL
                     YTAMVDNSGKIVAKSRPFFFIKEARAITTTADAGWQPAVEDMGFLYNHYKLVLSFVVL
                     LTILAAITASGGFILAERRRLTLGSLFDPTLPETPPQTETPSQTGAPPQTP"
     gene            5075..6973
                     /locus_tag="VI981_05055"
     CDS             5075..6973
                     /locus_tag="VI981_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEY4479692.1"
                     /translation="MLNPEIDKYVSEARAKGIPGDAIRQNLTAAGWSPTDVESVFKPT
                     SNPITLPVEQFSTSTVNKNGTSTKRHHYISPAIFFIVTLGLGILLVYQQDSDRSKTVF
                     VGLFVTALLLTAVFALVSLIIRKIGGRFGKKWAVFSWVFIVLMLVVAYIYALGRAGVP
                     LSSFGFFQQRDLNKTLSQDWTTYKNELHQVTLDYPPTWSVNDTSDQTKLSVLFQSPTK
                     GEAFGIDTVFIGGFLNLPAPTLDELVKIYLDQNKKVSSPQIEPTVSDYKIGNTSGKKV
                     QYMTKNSTGSRVTSTAILFMRGRQIVKFTYSAVASLKNSDLLLEKVLGSLVIEYKTPK
                     EVTIDTSDWKDYSNIDAGFSVLHPGNNKPYESLTFGDPKSVSFSFKIPLSTTQVPEKD
                     VNSSVVVERWSKDTCQEHSVSVASSSQGDNISLQNGINWYGIYSDVLFASTAGRGYQE
                     IYTSPIINSDHCYRIEMTYVPDLLNDDSRSQGHSVFIEVLKSFKEIDSVTKPTKNSSS
                     TPKAPVTDINTSVTGNPVAPSQAASYEDLVIGRNRVLSWTGPTNTTYTLVVTDVKSGE
                     LMGGIANTNGVENGVEHQWAWVVGSYYTFVNPTTKNLLPGTYKIEATYVGSNSEYMRQ
                     LVGVYNLK"
BASE COUNT         1948 a         1521 c         1680 g         2199 t
ORIGIN      
        1 tttttgttgt tatcctggcc ggtgtcctgg aactcgtcat cttctcattg gctgggcttc
       61 cgacaaatct tctcgggcag gttacacaac tcgattggag gatactctcc ttgggagcga
      121 tcattatcgg tattgtcgag gaggtcgtaa aatatgccgt ccttcgtgcc actgtcttct
      181 atagccatga cttcaatcaa atttttgacg gcatcgtgta cggcatcacg gtcgcgctcg
      241 gattttccat agctgaaaat attttgtatt ttttggagtt ttccgagtac tcgactggaa
      301 actttatttt cgctgctctt tttcgagctc ttttcagcac gctccttcac gtgatgacag
      361 cgggaatcat gggctactat cttgggaaag ccaagttttc tctcaataat aaaaagatgt
      421 atatcgtaac aggtcttgtc tcggctattc tcatacacgc gatttttgat tttgttattt
      481 tttcctcagt gacgatcgtt actttgtgca cctttattgc gcttatcggc gctttcggcg
      541 tcttcactcg cctctggact caccctgaag tgcgcatgat ctggcgcttt gacctccaac
      601 aagggaaata gtctcttttc gtctttaacg tttttatact tcatactcta aactgaatac
      661 tttatactgt tatatatgct ttacacgcga aaaggggaca aaggaaaaac gacgctctat
      721 ggttgtaatc agtcgatttc aaagagctcg gctatagcag aagcgctcgg tgcgcttgat
      781 gagataaatt catttcttgg gttttgcaaa gtccgatcgg caaaagcgaa actcacagtt
      841 ggcaaacaaa aatgttcgga aataatccac aatttacagg aaactctctt tataattcag
      901 gcagaagtgg cagggtttcc gatgaatgtc gatgggaaaa aagtgaaaga tattgaggcg
      961 acaatcgcgg ccattgaaaa acttttaccg cccataaaaa ccttttttat ttctggcggt
     1021 tcggaacttg cggcttcgtt tgactatgcg cgtacgattg ctcgacgtgc tgaaagacgg
     1081 gttgttgggg tcagtgaaga agataaaata aaaatagggg aacaaacgct tgcatatttg
     1141 aatcggctct caagtattct ctacgcgttt gcgcgtctag caaatattga cgcgaagaaa
     1201 aacgaaaaac caccgagcta taaataaagc cgcaggcaat gtcttgtatg ttactttaca
     1261 catctcacgc ctaacaggtt tcactttgtc ggtttttttg ttattctcga tctgtcggtt
     1321 gatggtgact atggtgtaat agcagcactg acccctgtgg agggtccaga atgggtgcaa
     1381 atcccattag tcaccccaaa tgaaagccta tcttgcaatt aaatatcaca aggatggcag
     1441 aaatcacgga ctgattcgtg aaatttgtga ggtgcttcgt gcactggaga ttgaaacgat
     1501 agcaccagtt attcaccttg agacagacgg taaaaaactt gaaccgaaag aactcatgca
     1561 gaagagcctt gccgaactcc gcacctgtga cattttgatt gccgaatgtt ccgaaaaaag
     1621 cgttggactc ggaattgaag caggctatgc attctcgata gcaaaaccaa tcattcttat
     1681 cgctaaaggg ggatcagaag tttcccccac acttctcgga attgcggaga agataatttt
     1741 ttacaataat cccgcggagc tttctcggaa aattagatgg tagcattatt ggctgtgcac
     1801 aatggggaat gagacatatg catgagttcc gacacctttt attgattcga tgtagattga
     1861 accgctcatc ttgaggacta attggcgcgt aatccagaga ccgagcccgg taccccctat
     1921 ctcttttgtc tcctctgatt tgacgcgata aaacttttca aatatttttc cctgctcctg
     1981 ctttgtcatc ccgatcccgg tatctgccac acaaattacc actcgattat tttcgcgctt
     2041 ttgagtaact tttatgttgc ctttttgcgt gtatttaatg gcattgccaa ttaaatttac
     2101 gaacacctgt cgtaggcgat ctcggtcgac gagtgctaag agatcgtctt gtatttccac
     2161 ggcaagagag agtcctttgt cggaagctac ttttttaaga gaatcgacca catcggacac
     2221 gagctcacct gtcttgcaat ttgcaagttt tacctcgatt cgcccctgtt cgattcgagc
     2281 aacgtccaga atatcggcaa cgagcgagtc aagattccgg acagacgtca gaatattctt
     2341 cgaaaaaatt tgcttgtcat tttcttctga ctccctatca agaaggagtt ctgcgtaacc
     2401 tttaattgcc gcgagtgggg tgcgcagttc atgagaagcc atggcaatga aatcgtcttt
     2461 caatgtgtca atttcacgaa gtcgtttgta taagacaccg taatctataa tcctagagtg
     2521 cctgaaaaaa agtaacaata cggcaaaaag gatgagaaac aggatgaata tgctcgcccg
     2581 aatttccgat gcgatttttt tgtccgcttc cgacagggac tgcacggtca cgagcatttt
     2641 tggctcaccc gacacgtccg caatagcgcg tgccgtctta aaaaaacgct caccgttttc
     2701 ataccgttcg gtggtaaagg atcgagatgg atctgatttt gcgagattgt atagaaaagc
     2761 atcttctgga gcaatttcgc ttggcgtccc tttctcggct gaaaggtcaa gcgaagcgta
     2821 gagtgtttcc ttgccgtttt caattccaat cagtttaaat tgggagattg ttgtattatc
     2881 gttcgcgacg ttccggaaca tagtctctat gtctgtagaa tacggactat ggagccgtac
     2941 ataggccgcg aaggcatcat gtatcgagcc gactcggacg ttaatcaggt tgtcttcggc
     3001 gctttttgca attccgataa atcggttgga gacaaagatg aaagagaacg caataatgag
     3061 cgcgacaaga atagagtacg cgagttgcgg gttttcccgc agtcttgcga aaccggcctt
     3121 cagtgaattc ccgagtgcgt tcatggagtt tgaggtggag ccccggtttg ggagggggtt
     3181 tcggtttgag gcggagtttc gggcaaagta ggatcgaata gactcccaag agtcaggcgg
     3241 cgtcgctcgg ctagaatgaa gcctccgcta gctgttatgg ccgcgaggat cgtaagcaat
     3301 acaacgaatg agagtacaag tttgtagtga ttatagagga agcccatatc ttcaacggcc
     3361 ggttgccatc cggcatcggc ggtggtagtg attgctcggg cctcttttat aaagaagaac
     3421 ggtcggctct ttgcgactat ttttccggaa ttatctacca tcgccgtata cagttcgtgc
     3481 gagccgtccg ccaattcttt ctcgagtgta taggtccact caccgctaaa attggtttta
     3541 acagtgacga taaccggtgt gctgtaaata aaaatggtga caaagctatt cggcagtgca
     3601 cgcccgctca tttgaatttt gcctttgtga tctgtttcgg agagatcgac aatttcgata
     3661 ttttttatgg agaagagatc gggtgctacc ttgccgcgtt cgtgaggatc gtcataaatc
     3721 atgagggcac tggtggtgct taagggatca attcctttca ataccttttc ggcgtccgta
     3781 agttctcccg gagtcgtggt ggcatttttg gggtcggttc catagatata gcgctcgtcg
     3841 aaatctgata tgccgtctct gtcctcgtct ctgtaggcgc ttagcgaatc tttttggcga
     3901 ccttcgactg cgtatttttt tattacatca ttaagttttt caacgtatgc cactatgtcc
     3961 tttttattct gttcggttga gattgtgcca tttgtttttt ctttgacgat acgctcgatt
     4021 tctttgaaag atgtttcaac attttgttct attttcttaa tatcgatttc tggggttcta
     4081 agagttgatg tgtcttccaa tgcacgctct atatcaatgc gaacgtcatt aatcagattt
     4141 tccttcactt catttacgct cgcattaatt tctgtacgtg tagaatcgct cactgttgaa
     4201 cttgcaagcg ttgtttgcaa tgtctggtct atctcgctgg cgatggcatc gagtgatttg
     4261 ttggtttcgg tttgaatttc cagagtagaa gtgtttcgat cacgtagatc ttcaatcgcc
     4321 ccgtcttctg tggtggatga tggtcctacg tttagagtgc tacttgccgt agtcttatct
     4381 tctgttgaag tttgcgaagt gtctgtgtcc gttgtggacg gaggtgaagc aagaggtgaa
     4441 actaatgagc cgtcgccaga cgaagaatct gaagttgact gaatttgcga gctgccgtca
     4501 ctcgtacttg tgcttaccga attagtatcg gtacgaaaca gtgctccatt ttcgtacgat
     4561 gacgcggtat ttgccaagag tagaaaataa aaaacgaagg aaagcgcaaa taggattttt
     4621 ttaaaaaatg taattttttt ctgcatattt tgacctcttc tcgaggcgcg atgcatgatc
     4681 aattataagg taggtaatta ttaaaaacta tgtattttct gggacagcac gttgaaacgg
     4741 aaaacgagag aatgttcgca cgaaaatagt catgatcgga tagtggggca gcccgcaaac
     4801 ttattttggc tagcgaggat cgggtgaacg tgtcttcatg ttcacatcgt ccgagcgaag
     4861 acaaaatata tgattggagt cacatataat tacgtcgttc ggtcgagatg agagagcaga
     4921 ctctctcatc cctccctctc tagtaattat gagacgaaca aagcgagtgc tcttattttc
     4981 aatatttcga ggccgtgaca gctcgctggg agaatattta gttgtcaacc tctgaaggga
     5041 ataggtagtt taaattatga tattatttta ggacatgtta aatccagaaa ttgataaata
     5101 tgtttccgaa gcgagggcga agggtattcc cggcgatgct attcgtcaaa acttaacagc
     5161 ggcagggtgg agtcctactg atgttgaaag tgtttttaaa ccgacatcaa acccgataac
     5221 gctcccagtt gagcagtttt caaccagcac agtaaacaaa aatggcacat caaccaagag
     5281 acatcactac attagcccgg caatcttttt tattgtaacc cttgggttag gtattttgct
     5341 tgtctaccaa caggattctg acaggtccaa aactgttttt gttggtctgt ttgtaacagc
     5401 gcttttactt acagcggttt ttgctttggt atctttgata attagaaaga ttggaggacg
     5461 atttggaaaa aagtgggccg ttttttcttg ggtatttatt gttcttatgc tggttgttgc
     5521 ttacatatac gcgttgggaa gggcgggtgt gccgctttca agttttggct tttttcagca
     5581 acgagatctg aacaaaacgc tttcccaaga ttggacgact tataagaacg aactgcatca
     5641 agtgacactt gattatccac ctacttggtc ggtgaatgac acatctgatc aaaccaaact
     5701 ttctgtattg tttcagagcc caaccaaagg tgaggcgttt ggcatcgaca ccgtttttat
     5761 aggtggtttt ttgaatttac ccgcacccac attagatgag ctagtcaaaa tttatcttga
     5821 tcaaaacaag aaagtatcat ctccacagat agaaccaact gtttccgatt acaagattgg
     5881 gaatacctcc ggaaagaaag ttcaatatat gacgaaaaat tccacaggaa gtcgtgtaac
     5941 gtcgactgcc attcttttca tgagaggtcg ccagatagta aagtttacat attcagccgt
     6001 cgcaagctta aaaaattctg atttgttgct ggaaaaagta cttggttcgt tggtgatcga
     6061 atataagacc ccgaaagaag taacaataga cacttcagat tggaaagatt attccaatat
     6121 agatgctggg tttagtgtcc tacatccagg aaataacaaa ccatatgagt ctttaacgtt
     6181 tggagatccg aaatcagtat cattttcctt caaaatacct ctttcgacga cacaggtgcc
     6241 tgagaaagac gtaaattcat ctgtcgtcgt tgaaaggtgg tccaaggata cctgtcagga
     6301 acactctgtg tcggtggctt cctcgtcgca aggcgacaat atcagtttgc agaatggcat
     6361 aaactggtac ggaatttata gcgacgtcct gtttgcttca actgcaggcc gtggatatca
     6421 agaaatctat accagtccga ttatcaactc tgaccactgt taccgcatag agatgacata
     6481 tgttccagac ctgttaaatg atgattcgcg tagccaaggg cactctgtgt ttatcgaggt
     6541 gttaaagagt ttcaaagaaa ttgattcagt caccaaacct acgaaaaact cttcaagtac
     6601 accaaaggcc ccagttactg acataaatac atctgtaaca ggcaacccag tggctccatc
     6661 tcaagcggca agctatgaag acttagttat tggaagaaat cgagtactgt cgtggactgg
     6721 tccgacaaac acaacttaca cacttgtggt aacagacgtg aaaagtggcg aattgatggg
     6781 tggtattgct aacactaacg gcgtcgagaa tggggttgaa catcaatggg cctgggttgt
     6841 gggaagttat tacacctttg ttaatccaac aacaaagaac cttctccctg gtacttataa
     6901 aatagaggca acctatgtgg ggagtaattc tgagtacatg aggcagttgg taggggtcta
     6961 taatctaaaa taaaactaga tatatctgcc tcatggaagc tcgtggggag aatatttagt
     7021 tgtctacctt tgaaagggtt aggctgttct aactttgttg ttcggaactt tgaattacga
     7081 aactcgccag cccctcgatt gtccactcac gtaaaagaca tctccagtct tctggagtga
     7141 cacaaaagtc tttttcttca gctacgtcac tctaacgcaa cacatacgcc agaagtgagt
     7201 acacataatt gaagtgtacc ccttttctta gacagttagt ttctcttgac ttgtagtgta
     7261 tttgatttct ttctcttgcg caaatttttc tgtgaatttc tttggcgcca ttcccagact
     7321 cgtgtgaatg cgtaagttgt tgtaataa
//
LOCUS       DATDSU010000145         2018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1146685, whole genome shotgun sequence.
ACCESSION   DATDSU010000145 DATDSU010000000
VERSION     DATDSU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146685"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VI981_05060"
     CDS             <1..448
                     /locus_tag="VI981_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4479693.1"
                     /translation="FQEMLEIFSRSKINLNLNPPMSAISMKSLAQVFFRRRRKWIVPD
                     FWHFPRNLQSYFQKKIPQIKARPFEITGCGGFCITGAADDTENYFVPGKEIVIYENTK
                     DLVEKIRYYLAHDDERKTIEKAGYERAVREHTYKARLDDIFRQLGL"
     gene            complement(506..1081)
                     /locus_tag="VI981_05065"
     CDS             complement(506..1081)
                     /locus_tag="VI981_05065"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4479694.1"
                     /translation="MQQNTLSHMGWGLWANLTRRIGPGRIPQKAFDAFCGAFVTNSVN
                     LIPIGVDGFGEAEVLLIWREDEYCHGWHFPGKILLPGQTSEGTLDETIETELGPDAKV
                     EILSLLGVFDIMKGTGVGESPRGQEVSQVYIGLVRFVEDIAPDDGEWEWFPLDDLSRD
                     LIDEQRPHLEAARVWVRNNQARLQEEDPIFQ"
     gene            complement(1159..>2018)
                     /locus_tag="VI981_05070"
     CDS             complement(1159..>2018)
                     /locus_tag="VI981_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479695.1"
                     /translation="TLGLPLLFVAIFLPFAFAFETFEFFWQGRGRFDIQSKYRLATTI
                     IASTVLVSTAFLTDNLIALVLAFFASRAILHAFFYLLSLKSVANEEEDKETISLGKHL
                     TAMGTVSIVGAQLESIILWHFLGPVAVAVFSFARLPIANMQGLIPIDRLALPKLSERN
                     VAEDKKNIFSKFFKLFVVAIPLIVISILSASLIFKLFFPTYLEAVPYFRLLTLTFLWI
                     PFALLDISLVAAGRKKSLYISQTSVPIAKIVLLLILTPLYGIWGAVAAIVLETVFRAG
                     TSFYLFKRL"
BASE COUNT          559 a          470 c          486 g          503 t
ORIGIN      
        1 ctttcaagag atgcttgaga tattttcacg aagcaaaatc aacttgaatt tgaatccacc
       61 gatgtctgcg atctctatga agtcgcttgc gcaagtattt tttagacgcc gtcgtaagtg
      121 gattgttccg gatttttggc actttcctag aaacctgcaa tcctacttcc agaaaaagat
      181 tccgcagata aaagcgaggc cgtttgaaat caccgggtgc ggcggattct gtattaccgg
      241 tgcggcagat gacacggaga attattttgt tcccggcaaa gaaatagtga tatacgaaaa
      301 taccaaagac ttggttgaaa agattcgtta ctacttagct cacgatgatg aacgaaagac
      361 aattgagaaa gctggatacg aacgtgcagt tcgagaacat acatataaag ctcgcctcga
      421 cgatattttt agacaactcg gtctctaatt tagaaaaaag cgtgctccgg cggtcaagtt
      481 accgccggag cttgttgcct ctccactact ggaagatcgg atcctcctct tgaagtctgg
      541 cttggttgtt gcgtacccat acccttgccg cctcgaggtg aggcctctgt tcatctatga
      601 ggtccctgga taggtcatcg agtgggaacc actcccactc accgtcgtcc ggtgcgatat
      661 cctcgacgaa tctcacgagg ccgatgtaga cttgtgagac ttcctgaccc cgaggactct
      721 cgccgacccc ggtgcccttc atgatgtcga agacaccaag aaggctgagg atttccacct
      781 tcgcatcagg tccgagttcc gtctcgatcg tctcgtcgag agtcccttcc gatgtttggc
      841 ctggaaggag tatcttccca gggaaatgcc atccgtggca gtactcatcc tcgcgccaga
      901 taaggagaac ctcggcttcg ccgaacccat cgactccaat cggaatcagg ttcacagaat
      961 tggtgacgaa cgccccgcag aaggcgtcga atgccttctg cggaattcgg ccgggtccga
     1021 tccgtctcgt caggtttgcc cataggcccc accccatgtg cgaaagtgtg ttctgctgca
     1081 aaagagcctc cgttgttggt aagcactcat tgcaccacaa agcctttgta ctgtcaatat
     1141 gtttgccgtg tctcttatct atagtctctt gaataagtag aaagatgtgc ccgcgcgaaa
     1201 aacggtttct agtactattg ccgcaacagc accccagatg ccataaagcg gcgtgaggat
     1261 aagaagcaga acgatttttg cgattgggac ggatgtctgt gaaatataga gtgacttttt
     1321 tcttcctgcg gcaaccaaag atatatcaag cagcgcgaat ggtatccaca gaaaagttag
     1381 agtgagaagt ctgaaatacg ggacagcctc aagataggtt ggaaaaaaca atttgaaaat
     1441 tagggatgcc gagaggattg aaataactat aagcgggata gccacaacaa aaagcttaaa
     1501 gaatttacta aaaatatttt ttttgtcctc cgcaacgttc cgttcgctta gtttaggcag
     1561 ggccagtctg tctatcggga ttaatccctg catattcgcg atagggagac gagcaaaaga
     1621 aaatacagca acggcaacgg gtcccaaaaa gtgccataaa ataatactct cgagctgagc
     1681 gcctacaatt gacactgtcc ccattgcggt aaggtgtttg ccgagcgaga ttgtttcttt
     1741 gtcttcctct tcatttgcga cgctctttaa agaaagtaaa tagaaaaacg cgtgaagtat
     1801 tgcgcgtgat gcaaagaatg ctaataccag agcgattaaa ttgtctgtaa gaaatgccgt
     1861 tgacacaaga acagtcgaag cgattattgt ggttgcgagt cggtacttac tttgtatatc
     1921 gaatcttcct ctgccctgcc agaaaaactc aaaagtttcg aaggcgaacg cgaatggcag
     1981 gaaaatcgct acgaaaagaa gaggcaatcc gagagttg
//
LOCUS       DATDSU010000146         4475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_487678, whole genome shotgun sequence.
ACCESSION   DATDSU010000146 DATDSU010000000
VERSION     DATDSU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487678"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VI981_05075"
     CDS             <1..677
                     /locus_tag="VI981_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY4479696.1"
                     /translation="GYAQEMEQVGQRILAAGGGAGGKGNFKFRSAINTSPVEFQEGLA
                     GDSVLYTLKLRLIADIGLVGLPNAGKSTLLNELTAAKSKVANYAFTTLEPHLGAYYEL
                     ILADIPGLIEGASSGKGLGVKFLKHIERTKVLFHLVSAESEDPVKDHAVIRKELTAYS
                     PQLAEKTEYVFLTKSDLVSADVLSDKLSALTKAGIAATPFSVLDNESLGEVKKILNKI
                     KEGKGS"
     gene            748..2946
                     /locus_tag="VI981_05080"
     CDS             748..2946
                     /locus_tag="VI981_05080"
                     /inference="COORDINATES: protein motif:HMM:NF025438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEY4479697.1"
                     /translation="MAWKLPSPAKKKRIETKDKAEAARLKKDGYLQKKLKSGRIVLEK
                     KFAKWELFQEEVRVFLEALGFQDTESGHSSWLGRYQIDVVGGIEGTFLVFECKSSHDP
                     KLSKTLTKEINVFAGKKIEIEKAIREKYGAKYNEVKFVLALEDVHPTEDDIKTAREND
                     IYLWGSSYLKTGKDLFTIIGPLTAHYVLKELGVSPKTIRDEEGGADYKVPSFCVTVGE
                     QKIYSFFLPAEKLLNLVYVFRLQPGNEDAYQRFISKKRILGTGGITQFIDSGRFFKTS
                     VVCSFERQIKFDPKPTGLLLSSKDVEFGILSIPKLYGTVWIIDGQHRIYGYAGVNPEM
                     RKTNIGVVAYQEVDKRQQARDFIDINQKQKPVDPNTLWDLLSQTDPYSTEGAITKIAK
                     ELNNKKGIFKGKVLIPGKSFHAKRSSYPLKLSNICLSLSDRRLVDFNVRDNLYKTTSD
                     VRDDNRYPDSVIEHSVNVLDEYFLLLWEIAEKVPDWRKGFILNNNGFNVFLRVLAEIL
                     KFQSGNWSKQEAKKILTGPIESYFEAGYEDLSDIRTFTSNEAGRAKEALDIIKRINEK
                     NETFARDFIKEAERRERLAYEKSDPYQILKDLESNLRQFIENKLSQATDHWWKQRIPK
                     DVQEKAELKLEKSQSPWPWVKAEDKYPIAYIDFPDYAKIILRADNRRHLFPEIKSDAP
                     IESIVTKLKELEDIRNKIAHSRPITVQESTTLRLYTTQILQTINPHSEPS"
     gene            3009..3365
                     /locus_tag="VI981_05085"
     CDS             3009..3365
                     /locus_tag="VI981_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479698.1"
                     /translation="MSEAKMKEYKARLPLRVQISIKNTDEGLWAKISTTDGKIDNCYT
                     QASSATELLLMVNDAILTYFEIPEDSRKDVGFYVPLSKQHVRIEDMFNQFVSMEKEID
                     QRGRSEKTLTLQNSVC"
     gene            3757..3966
                     /locus_tag="VI981_05090"
     CDS             3757..3966
                     /locus_tag="VI981_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479699.1"
                     /translation="MESPHELSLENIPEERQVGGEAEHYGSLYLHLQKQIHKNARTSD
                     TVPAVHRRSNGHSKKERRIAALVIL"
     gene            4019..>4475
                     /locus_tag="VI981_05095"
     CDS             4019..>4475
                     /locus_tag="VI981_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4479700.1"
                     /translation="MEIRNIAIIAHVDHGKTTLTDALLRQTGVAEEGVSMDSNALELE
                     RGITIYSKNAAIFYKDTKINIVDTPGHADFGSEVERVLRSIDSVLLVVDAQEGPMPQT
                     RFVLKKSLELGLKPIVVINKIDKPAADPARTEEQVLELFLELGASDAQAD"
BASE COUNT         1368 a          984 c         1068 g         1055 t
ORIGIN      
        1 cgggctacgc gcaggaaatg gaacaggtgg ggcagaggat tctcgccgcg ggcggaggcg
       61 cgggcggcaa gggaaatttc aaattccgct ccgccatcaa cacctcgccg gtggaatttc
      121 aggaagggct cgcgggagac agcgtgctct acactctcaa gctccggctc atcgccgaca
      181 tcggacttgt cggactgccc aatgcgggca agtcgacact tctgaacgag ctcaccgccg
      241 cgaaaagcaa agtcgccaat tatgcgttca cgacactcga gccgcatctc ggcgcgtact
      301 acgagttgat tctcgcggac atacccggcc ttatcgaggg ggcctcaagc gggaaggggc
      361 tcggcgtgaa attcctgaag catatcgagc gcacgaaggt cttgttccat ctcgtgtccg
      421 ccgaatccga agatcccgtg aaagaccatg cggtcatcag gaaagaactc accgcgtaca
      481 gcccgcagct cgcggaaaaa acggaatatg tctttcttac aaaaagtgat ctcgtgtctg
      541 cggatgttct ctccgataaa ctctcggcgc tcacgaaggc gggtatcgca gcgaccccct
      601 tttccgtctt ggataatgag agcctcggcg aagtgaaaaa gatactcaac aagataaaag
      661 aggggaaggg ttcctgaaat tttttctccg aagtaatgca cgttatgcaa ttgaaaaagg
      721 tcataaaata agctattgta cccttgtatg gcttggaaat taccatctcc agcgaagaag
      781 aaaagaattg aaaccaaaga caaggccgag gcggcccgac ttaaaaaaga cggttactta
      841 caaaagaagc taaaaagtgg aaggattgtc ttagaaaaga aatttgcaaa atgggaactt
      901 ttccaagagg aggtgagagt ctttctcgaa gctttaggat ttcaagatac cgaatcagga
      961 cattcttcct ggctcggtag ataccaaatc gatgtcgtcg gaggcattga gggaaccttc
     1021 cttgtttttg agtgcaaaag cagtcatgat ccgaagctat caaaaacact cactaaagag
     1081 atcaatgttt ttgcaggcaa gaaaatagaa attgagaagg ccattcgtga aaagtatgga
     1141 gcgaaataca acgaggtgaa attcgtcctt gctctcgaag acgtacatcc tacagaggac
     1201 gacataaaaa ctgcgcgaga aaacgatatt tacctttggg gcagtagtta tcttaaaacg
     1261 ggtaaagacc tcttcacgat aattggaccg ctaactgctc actatgtcct taaagaactc
     1321 ggagtgagcc caaagacaat ccgtgatgaa gaagggggtg ctgattacaa agtcccaagt
     1381 ttttgcgtaa ctgttggcga gcaaaaaata tacagtttct ttttgccggc agaaaaatta
     1441 cttaatctgg tatacgtttt tcgcctccag cctggtaatg aggatgcata ccaacgattc
     1501 atatccaaaa aaagaatttt aggtactgga ggcataacac aatttataga tagcggtcgg
     1561 tttttcaaga ccagtgttgt atgtagcttc gaaagacaga taaaatttga tccgaagcca
     1621 acgggtcttc tattgtcctc aaaagacgtt gagttcggaa ttctaagcat tccgaaacta
     1681 tatggaacag tttggattat tgatggacaa cacagaattt atggttatgc cggagtcaat
     1741 cctgaaatga gaaaaactaa tatcggagtc gtcgcttacc aagaggtaga caagaggcaa
     1801 caggcgagag atttcatcga catcaatcaa aaacaaaagc ccgtagaccc gaacacactt
     1861 tgggatctac tttcacaaac cgatccatac tcgacggaag gagcgattac gaaaatcgca
     1921 aaagaactaa ataataaaaa agggattttt aaaggaaaag tattgatccc aggaaagtct
     1981 ttccatgcaa aaagatcatc ctacccactt aaattgtcga atatctgctt gagtttgtct
     2041 gatcgcagac ttgtagactt caacgttaga gacaacttat ataaaacgac aagtgatgtt
     2101 cgtgatgata accgttatcc ggactcggtc attgaacatt cagtcaatgt gcttgatgaa
     2161 tacttcttgc ttctatggga aatcgctgaa aaagtccccg attggagaaa agggtttatt
     2221 ttaaataata acggattcaa tgtttttcta cgcgttctgg cagaaatctt aaagtttcaa
     2281 tctggcaact ggagtaaaca ggaagcgaag aaaatactga cgggaccgat tgagagttat
     2341 ttcgaagcag gctatgagga tctatccgac attagaacgt ttaccagtaa tgaagccggt
     2401 cgagcgaagg aagccttaga tattatcaaa aggatcaatg agaaaaatga aacctttgct
     2461 cgcgacttta tcaaagaagc agaacgacgt gaacggcttg cgtacgagaa gtcagatccg
     2521 tatcaaatcc tcaaagatct ggagtcaaac ctgcgtcaat tcatagaaaa caaattatca
     2581 caggcgactg accattggtg gaaacaacga atacccaaag acgttcaaga aaaggccgag
     2641 ctaaagttag aaaaaagtca gagcccttgg ccgtgggtta aggctgaaga caagtacccc
     2701 attgcatata ttgactttcc ggactatgca aaaatcattc taagggcgga taatcggcga
     2761 catttattcc cagagatcaa atccgatgcc cccatcgaat ccatcgttac aaagttgaaa
     2821 gagctggaag atattagaaa caaaattgcc cattcaagac caataactgt tcaagagtcg
     2881 acaacgttgc gactttacac cacacaaatt ttgcagacta taaatccaca tagtgagcca
     2941 tcataaacgt tatcaacagc gtttcgtctc cactggaaat tcatccacca gcgcgctata
     3001 ctcacgacat gagtgaggca aaaatgaaag aatataaggc tcggttgccg ctacgagtac
     3061 aaatcagcat caaaaatacc gatgagggtc tttgggctaa gatttcaact acagacggca
     3121 aaattgataa ctgctatacg caagcaagca gtgctacaga attactgctg atggttaacg
     3181 atgccattct gacgtacttt gagatacccg aggattctag aaaggatgta gggttttatg
     3241 tgccactttc caaacagcat gttcgtattg aggatatgtt taatcaattt gtctcaatgg
     3301 agaaagagat agaccaacgc ggtcgtagcg aaaaaacatt aacattgcag aactctgttt
     3361 gttgatctcg gcgtgtggcc aaacctaaag ggggaaaacc caaactggca gcttgtacac
     3421 ccgatgatgt tttcggggcg atacataaac ttggcggctt ccaaataatc tttgagagtg
     3481 cgaagcatag caagattcgg catatagcgt ccggtcgggc attcatgatt cctcgaactt
     3541 caccggtgaa taagcacatg ctacgagatt ttgtggagaa attccttgtc gcaaaatgcg
     3601 gacttgaaga gaaggagatt tacaaacacc tctggtgtta aagtgcccta catattattg
     3661 cctaacggcg gatttgattt ttcggcgcgg ctgtggtatc attaggatta ctttttcaga
     3721 tcctccgcgc tccgggtagt ttgtgcggct tgtccgatgg agtcaccgca tgaactttcc
     3781 ctcgaaaaca taccagaaga acggcaggtg ggtggagaag ccgagcacta cggtagtctc
     3841 tatctgcatc tgcaaaaaca aatacataaa aacgcgcgaa catcagacac agtgcctgcg
     3901 gtgcatcggc gtagcaatgg ccatagcaaa aaagaacgca ggattgccgc tctcgtaatc
     3961 ttgtagtacg atgcacttac gtgcctcggc aacgaggcat tttgctttca ctatttgtat
     4021 ggaaatcaga aatattgcaa ttattgcgca cgtcgaccac gggaagacta cgcttaccga
     4081 tgcgcttttg cggcagacgg gcgtcgccga ggagggtgtc tctatggact ccaacgcgct
     4141 cgagctcgag cgcggaatta ctatctactc gaagaatgcc gctattttct ataaagatac
     4201 gaagataaat atcgtcgata cccccggcca cgcggatttc ggttccgaag tcgagcgtgt
     4261 gctccgttca attgactccg tcctccttgt agtggatgcg caggagggtc ccatgccgca
     4321 gacacgcttc gtactgaaga aatcgcttga actggggctc aagcctatcg tggtcatcaa
     4381 caagatagac aagcccgcgg cggatcccgc gcgcaccgag gagcaggtct tggaactttt
     4441 cctcgaactc ggcgcctcgg acgcgcaggc ggatt
//
LOCUS       DATDSU010000147        12791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_488254, whole genome shotgun sequence.
ACCESSION   DATDSU010000147 DATDSU010000000
VERSION     DATDSU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12791
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488254"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..138)
                     /locus_tag="VI981_05100"
     tRNA            complement(57..138)
                     /locus_tag="VI981_05100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Leu,seq:caa)
     gene            249..1238
                     /locus_tag="VI981_05105"
     CDS             249..1238
                     /locus_tag="VI981_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY4479701.1"
                     /translation="MSSNFYSDSIFWIEVDKIKPNPYQPRHEFDEARLKDLAESIRMY
                     GILQPLIVTRKEIYKEDGGLAVEYELVSGERRLRASKLAGLLQVPTLIRASEDDARVK
                     LELALIENVQREDLNPVDRAMAFDRLVKEFNFKHFQIAEKIGKSREYVTNSIRLLALP
                     ENIIDSLQAGQISEGHARPLLMLIDRPEEQAVLFKDIILKKLTVREAERAARSIAVEK
                     QRKRMNALPDQEIQDMISKFTETLGTRVEIEQKRSGGKIMIDFFTADDLHHIYDLLKK
                     GPSAQMINEVAPNSVELPTTPTAPTTPIDDRAPADIKIEESDDELYSIKNFSL"
     gene            complement(1321..2874)
                     /locus_tag="VI981_05110"
     CDS             complement(1321..2874)
                     /locus_tag="VI981_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEY4479702.1"
                     /translation="MNHNRSIREIIDLMDSPSPEAIALITKAYKFADEAHKDHRRYSG
                     EPYFVHLVETAKILAELGKGPITIAAGLLHDCVEDTGVSEETIEKEFGKEILFLIQGV
                     TKLGQLRYRGVERHVDSLRKLFIAMAQDIRVLIIKLADRLHNMRTLEHVPEEKRHRIA
                     LETLEIYVPLAYRLGMRKLNRELEDLAFSYVYPEEYERVSKLIKEKTKESVEHLKKFH
                     KSLIKVLVKAGVTHITSDNRQKNLYSLYKKLQRRNDDIEKIYDIAALRICVPTIADCY
                     RVLGIVHGNWRPLPGRIKDYIAFPKPNGYRSIHTTVFTGDGSIVEVQIRTQEMHKEAE
                     YGIASHLSYKYKKGSDAGTLSWFRQLLPTMTSFESTHPALHESSRKSNEAVPNWIRHL
                     AETQDQFSEHNEFMDNLKADFFELRVFAFTPKGDVVDLPIGASPIDFAYAIHSDLGNH
                     ISGAKVNGKLVTLDTQIQNGDIVEIQSKKSAHPTTKWLAFTKTSLAKKHLKAELERLG
                     QFGQSKIAV"
     gene            3315..3686
                     /locus_tag="VI981_05115"
     CDS             3315..3686
                     /locus_tag="VI981_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479703.1"
                     /translation="MRYFVFFSSITCAALLPFFAEAQETKFSSPVVFVDNYTLLIGIF
                     IGLVASILTVVYSLRMGRSMVGDILRPIGVGMFFIVLGFLAVAIAWAEPPAPEIVHNL
                     LFTSGYIMMLIGVARARKLSS"
     gene            3757..5667
                     /locus_tag="VI981_05120"
     CDS             3757..5667
                     /locus_tag="VI981_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4479704.1"
                     /translation="MNLLEKAQKFLRWKEVPKRGTEEREAVQALFAELSKLSEHSHKT
                     KTLQERFAKLEGSPGSLHAYELVELYLEFEGLLSDPKNQNKQVRVGELRKTLANIISI
                     GFLPPPLALLFTETEVSQILLYEVGLERIVSYVHSTLGADRAVRIISRYNERHVIDGV
                     RIIDGSLRFSDFNNRVHLLKTPLSELETIFSGLFSELWNDLTKALGEKITRNIILRLV
                     TLFKNTYGSKAASIFLRIIPDDILEDEHLSYLNREELEQKIKKRTTELKSENEEIEQR
                     IINRTHELAIEKKKLDIITERMTSGVVLLDQGKHVLFANRAARAIIKVSPDVTDGGAV
                     LAQFLALFKDINLDEYFKQCYPDGSFLLPEIIANDCMYRISLNCLHDNEQHFSGMLIW
                     IEDVTEQKILEKKKADFVSLVTHQLRTPLSGLKWTLRMLINGDLGSLSNEQKVFLMKS
                     YESNERMISLVDNILGINRVESDVYEYHYMSTQIVDLIDNVLFEVSPVANKKRINIQF
                     IKDLKLPKAMIDSEKMRAVLQNLLENAVKYTLDGGSIKIETLFKDNFIRVSISDSGIG
                     IPKAQQGSVFSQFFRAQNAATVKAEGSGLGLYIVKGIVEKHHGKIWFDSVEGKGTTFY
                     FTVPAVQSGEQK"
     gene            5720..6529
                     /locus_tag="VI981_05125"
     CDS             5720..6529
                     /locus_tag="VI981_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4479705.1"
                     /translation="MSKVLIIEDDIFLTEVLEKKMNDAGYAVEIARDGGVGLTKLKQD
                     KPDIVLLDLALPVKGGFEILEEKMRDASIRSIPVIVISNSGQPLEIDRVLALNVKDYF
                     VKAQFDPDEVLAKVKLQLRDMDAVASLGDDEKKTEKEHTASLASKKIMWVEDDVFLSD
                     IIARKLSNEGSKLIHAANGNDVFDMAKKEKPDVILLDILLPGKDGFHVLEQLKAVSET
                     KEIPVILLSNLGQKSDIEKGEKLGAARFLVKATVTLDEIVDEIKKFAKVSK"
     gene            6683..9409
                     /gene="uvrA"
                     /locus_tag="VI981_05130"
     CDS             6683..9409
                     /gene="uvrA"
                     /locus_tag="VI981_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY4479706.1"
                     /translation="MPKEKEKKDDLIIVKGARTHNLKNVTVEMPRNKMIVFTGLSGSG
                     KSSLAFDTIFAEGQRRYVESLSAYARQFLHQMQKPDVDEIVGLSPAISIDQKSRSNNP
                     RSTVATITEIYDYLRILYARIGRPHCLVCNREIKRLSHEEILETIIETVQATDISKAA
                     KDVLGVKISSLELKIYSPVVVGRKGEYYQLLYDLLDKGYSEVKVDGRVKKLREQIILE
                     KNKKHDIDVLVDKFFVTEFTDEKSGSAHSTSSRPAGSPQAGWRERLSEAIERALHESN
                     GLIKIETPNISRSDLETKDKPRPGLGDNQGLALAENDESHERLISSKFMCPYDGFSYP
                     EIEPRLFSFNSPYGACLECNGLGTKFFFGEEVCPKCNGARLREEALHVLVGGKNIVEL
                     VRLSIEETNRFFSHVKLTAVEINISKVVLKEIESRLTFMINVGIEYLSLDRRAHTLSG
                     GEAQRIRLASQLGSGLVGALYVLDEPTIGLHARDNDRLIKTLLNLRDIGNTIIVVEHD
                     EDTIFSSDYLVDIGPGAGTHGGKIVVADYLDPLITARKNESKSLTLDYLRGDKKIEFP
                     AERRTEERGKILIRGGSIFNIKDLNAEIPLGRLVALTGVSGSGKSSFMYEIVYKNLQA
                     RLERKYRSNKIWNCTSFAGTEYISRVILIDQSPIGRTPRSNPATYTGAFTHIRDMFAS
                     SEEARARGWKANRFSFNVKGGRCEACQGNGEIAVEMHFLPTVYVPCEVCQSKRFMKET
                     LEVKYKKKNIYDILKMTIEEAMDFFKDIPAISDRLETLNEVGLGYLELGQSATTLSGG
                     EAQRVKISSELYRPHVQKTIYLLDEPSIGLHYEDVRKLIEILEKLVKKGNTVMLIEHN
                     MDIIKCADWVIDIGPEGGDAGGKIVAKGTPEQVANVAKSYTGHYLKKILKRA"
     gene            9805..10737
                     /locus_tag="VI981_05135"
     CDS             9805..10737
                     /locus_tag="VI981_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4479707.1"
                     /translation="MEFTFSLAHISILAGSVIALIVASFFALSVVRRKMASSVNAGQK
                     NKIDTIKYEFVTVITHKFRTPLTQIKWGAEGLRAAATETQKEIEFRKIERAADTIIAM
                     TEVLLKVADADTSGIELQLEPVGVGEFVRSVANELSSDFMLKKVTPEFTQLSSTPSLV
                     RIDKKQMRLVFLTIMQNAATYVQPAGAVHVTFREERGFSITEVFDNGVGIPKDELARV
                     FDRFYRGAVAREIDQDGLGIGLYMSRKIINLHNGSLTLFSEGKGKGTVALIALPLAPL
                     PESAAGAAASAPIASPISVLVPPKNTRVAETVSH"
     gene            complement(11563..11913)
                     /locus_tag="VI981_05140"
     CDS             complement(11563..11913)
                     /locus_tag="VI981_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479708.1"
                     /translation="MNSEIPALRQSLALSFQPTDVVNGFSFTFASRLLSKLRDLKMHG
                     QMVRDGYLTETACALARNLDKQIYATERSEGSYQTNMSDHVRKLSVLMSTAEEFLATL
                     ADPPPIKPDGRDDL"
     gene            11955..>12791
                     /locus_tag="VI981_05145"
     CDS             11955..>12791
                     /locus_tag="VI981_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479709.1"
                     /translation="MTSQEMLKKKLPDVPGVYFFKCAGEIVYIGRATSLRDRLKSYFK
                     DDVIKTRGPLIVDMVTRADELDFITTESVLDAIILEANLIKKYQPHANAREKDDKSFN
                     YVVITDEKYPRVELMRSKDLESPLRAEGYPSTGRGSRVKSKDKLKAVFGPYPQGFILK
                     EGLKIIRRVFPYRDRKCVPESGQPCFNRQIGVCPGTCTREISKKDYNRTILHLKLFFE
                     GKKGELLKKLEREMKEFAKKREFEKANAMKKTTFALTHIQDLTLIKAESRETPLLSRA
                     ALD"
BASE COUNT         3669 a         2615 c         3063 g         3444 t
ORIGIN      
        1 taagcccggt aatactcaaa gataaatcaa ttcttcgcac aaaaaacaga tttttgtgcc
       61 gggagagaga atcgaactct catggattac tccgcacgat tttgagtcgt gtgcgtctac
      121 caattccgcc atcccggcgt gtatgaaaag tctactagaa taatgaaaaa agacaaaaga
      181 gagaatacag catctgagat agggtaggga gtgatttatt gagcgtagaa tgataaaata
      241 ctttcaacat gagctcaaat ttttattcag attcaatatt ttggattgag gtagacaaga
      301 taaaaccaaa tccgtaccag ccgcggcacg aatttgatga ggcacggttg aaagatttgg
      361 ctgaatcaat ccgtatgtac ggtattctcc agccgcttat cgtgacgagg aaagaaattt
      421 acaaggaaga cggcggactg gccgtcgagt atgaacttgt ctccggcgaa cgccgtttgc
      481 gcgcgtcaaa actggccgga cttttgcagg tgccgacgct cattcgtgca agcgaagatg
      541 acgcccgagt gaaactcgaa ctagcactca tcgaaaatgt tcagcgcgaa gatctcaatc
      601 ctgttgatcg ggcaatggca tttgatcgtc ttgttaaaga gtttaatttt aagcattttc
      661 aaattgcaga aaaaatcggc aagagccgag agtatgtaac gaacagcatt cggcttcttg
      721 cactgcctga aaatattatc gattcgcttc aagcgggcca gatttcggag ggccacgccc
      781 gcccgctact catgctcatt gatcggccgg aagagcaagc cgtactattt aaggatatca
      841 ttttgaaaaa attgactgtg cgagaggcgg aacgggctgc tcgaagcatc gccgttgaaa
      901 aacagcgaaa gagaatgaat gcactgccag atcaagaaat tcaggatatg ataagcaaat
      961 ttacagaaac gcttggcact cgggtagaga tcgaacaaaa aagaagcggc ggtaaaataa
     1021 tgattgactt ttttacggcc gatgacctac atcatattta tgatcttttg aaaaagggtc
     1081 cgagcgcgca aatgataaat gaagttgcac ctaattcggt tgaattgccg acaaccccga
     1141 cagcaccgac aacgcctata gacgatcggg cgccggctga tataaaaatt gaggagtcgg
     1201 acgacgaact atactctata aagaattttt cgctctagaa gttcaggctt ttttcggaac
     1261 acagcaacat cacgcggtgt gtaggatagt tgttgcgccg gtaggtgcga ggtctcgcag
     1321 ttatacggcg atttttgact ggccgaactg gccgagtctc tcaagttcag cttttaagtg
     1381 tttcttggca agtgatgttt tggtgaatgc aagccatttc gtggtcgggt gagcgctttt
     1441 tttcgactgg atttcaacta tatcgccgtt ctgaatttgc gtatctaggg tcaccaactt
     1501 tccatttact ttcgcacccg aaatatggtt tccgagatcg ctgtggatgg cataggcaaa
     1561 gtcaatcggg ctcgcgccga tcggcaggtc gacaacgtca ccttttggag taaacgcaaa
     1621 aacacgtagc tcaaaaaagt ctgcttttaa attgtccata aactcattgt gctcggaaaa
     1681 ttggtcttga gtttcagcaa ggtgacgaat ccaatttggc actgcttcgt ttgattttct
     1741 actcgactcg tgaagcgctg gatgggtgga ttcaaaagaa gtcattgtcg gtaatagttg
     1801 gcggaaccag gaaagtgtgc ccgcgtccga tccttttttg tatttgtaac taaggtgaga
     1861 cgcaataccg tattcggctt ctttatgcat ttcctgagtt ctgatctgta cttctacgat
     1921 acttccatct ccagtaaaaa ccgtagtatg aatacttcga tacccattcg gcttaggaaa
     1981 ggcgatgtag tcttttattc ggccgggaag cggacgccag ttgccgtgga caatgccaag
     2041 aacgcggtag caatcggcta tggtcggaac gcaaattcgc aatgcggcaa tgtcgtagat
     2101 tttttcgata tcgtcatttc gacgctgtaa ttttttatag agactgtaaa gatttttttg
     2161 gcgattatct gaagtaatgt gggtgacgcc ggctttgacg agcaccttga tgagcgattt
     2221 atgaaatttt ttcaagtgct cgactgattc tttggttttc tcttttatga gtttgctcac
     2281 acgctcgtat tcttcgggat agacatacga aaatgccaag tcttcgagct cgcgattgag
     2341 tttcctcata ccaaggcgat aggcaagcgg cacgtagatc tctagtgttt caagcgcgat
     2401 tctatgtctt ttttcctcgg gaacgtgttc gagggttcgc atgttgtgaa gccggtcagc
     2461 gagcttgatg atgagcactc tgatgtcctg cgccatggcg atgaaaagtt tacgcaagct
     2521 atcgacgtga cgctcgactc cccgatagcg caattgtccg agttttgtga ccccttggat
     2581 tagaaagaga atttctttgc cgaattcttt ttcgattgtc tcctcgctca cccccgtgtc
     2641 ttccacgcaa tcatgaagga gtccggcggc aatggtaatc ggccccttgc cgagttcggc
     2701 caggatcttt gcagtttcca cgagatggac aaaatatggt tcgccggaat atcgcctgtg
     2761 atctttatgg gcctcatcgg caaacttgta ggcttttgta atcagcgcaa tggcttccgg
     2821 ggagggagaa tccataagat cgataatctc tcttatgctt ctgttgtgat tcatcccgtt
     2881 tagaaacccg cctgccatcg cctgacacac tgatgggagg acgtgcgatt aattggtaat
     2941 atgatcatca ttttattaaa gctgccacat atttcgctct ggctgtggcg ggcaggtagg
     3001 aaatgcttct gcgtattcag aaagcactga ctatctatta gtttgaataa atttgatgct
     3061 ttgcatagca cagtcaaaaa gcatttccgt aacctatttt ctgatgagcg cacaagaccg
     3121 tctatattct cattgtccac aatttgatta tacataacca tatgatttct aacgggatgc
     3181 atatttttaa tgtatcacag aggaacgagc tgttcaaatc agcggaactt tcgtgtcctt
     3241 ttcgtccaca cgttctacgt ggggagtgag atcacttcag ggtataattt tgttattagc
     3301 attaagtgtt ttttatgagg tattttgttt ttttctctag cattacttgt gccgcacttc
     3361 taccgttttt tgccgaagcg caggaaacca aattttcgag tcctgttgtt tttgtcgaca
     3421 actatacgct cctaatagga atttttatag gccttgtcgc aagtattctc actgtcgtct
     3481 attcgcttcg aatgggcaga tcgatggtgg gtgacattct gcgtccaata ggggtcggga
     3541 tgtttttcat tgttctcgga tttttagccg tggcgattgc gtgggcggag ccgccggcac
     3601 cggaaatagt gcacaatctt ttatttacca gtggatatat catgatgttg atcggtgtcg
     3661 ctcgcgcaag aaaattatcg tcataattac aagattaagt ctacctgaaa atcagacagc
     3721 atggcctaac tacaaatgac aataatcata taaagtgtga atttactcga aaaagcccaa
     3781 aaatttttac ggtggaagga ggtgccaaag cgtggtaccg aggaacgaga ggctgtgcag
     3841 gctctttttg ccgagctctc aaagctttcc gaacatagtc ataaaacaaa gacgcttcaa
     3901 gagcgctttg caaaacttga gggttctccg ggttctctcc atgcgtatga gctcgtggag
     3961 ctttatctcg aattcgaggg attactttcc gacccaaaaa atcaaaacaa acaggtacgt
     4021 gtcggggaat tgcggaaaac gctcgcaaac ataatttcaa tcggttttct gcctccgccc
     4081 ctggcgctcc tatttacaga gaccgaggtc tcccaaattc tgctttatga agttggcctt
     4141 gagcggattg tgtcctatgt gcactcgacg ctcggagccg atcgcgcggt gagaataata
     4201 agcaggtaca acgaacgtca tgtgattgat ggagtcagga ttattgacgg atcgcttcga
     4261 tttagtgatt tcaataaccg agtacatctt ttaaaaactc cactttccga actggagact
     4321 attttttctg gcttgttctc tgaactctgg aatgatttga ccaaagcact cggggagaaa
     4381 atcactcgaa atatcatttt acgtctcgtt acgcttttta aaaacacgta tggatcgaaa
     4441 gcggcctcca ttttcctgcg aattattccg gatgatattc ttgaggatga gcatttatcc
     4501 tatttgaatc gagaagagct cgaacaaaaa atcaaaaagc gcacgactga attgaagtca
     4561 gaaaatgagg agatcgaaca aagaataata aatcgtacac atgaattagc gattgagaaa
     4621 aaaaagctcg atatcatcac tgaacgtatg acgtcgggag tggtattgct tgatcagggg
     4681 aagcatgttc tttttgccaa tagagccgcg agagcgatta tcaaagtttc tccagatgtc
     4741 acagatggcg gagcggtact ggcccagttt ttggctctat ttaaggacat taatcttgac
     4801 gagtatttta agcagtgcta tcccgacggg tcatttcttt tgcctgaaat aatcgccaac
     4861 gattgcatgt atcggatatc actgaattgt ttgcacgata atgagcaaca ttttagcgga
     4921 atgcttatct ggattgaaga tgtgaccgaa caaaaaatat tagagaagaa aaaagccgat
     4981 ttcgtgtcac tggtaacaca ccagcttcgg acgccgctct cgggacttaa atggacgctt
     5041 cgcatgctta ttaatggcga tctaggttcg ctctcaaatg agcaaaaagt ttttttgatg
     5101 aaaagttatg aaagcaacga gcgcatgata tctcttgtag ataatatatt gggcatcaat
     5161 cgtgtcgagt cggacgtgta tgagtatcat tacatgagta ctcaaatcgt ggatttgatt
     5221 gacaacgtgc tctttgaagt ttctccagtg gcgaataaaa aaagaatcaa catccagttt
     5281 attaaagatt tgaagcttcc aaaggcaatg attgattcgg aaaagatgag agctgtactg
     5341 caaaatttgc tcgaaaatgc tgtaaaatac acgcttgatg gcgggtcgat caagattgag
     5401 acgcttttta aggacaactt catacgagtg tcaattagtg atagcggtat tggcattcca
     5461 aaggcacagc aaggaagtgt tttctcccaa tttttcagag ctcaaaatgc ggcgactgta
     5521 aaagctgaag ggtctggcct tggcctctac atagtaaaag gcattgtgga aaaacatcat
     5581 ggaaaaatct ggtttgacag cgtagaagga aagggaacca ctttctactt taccgtaccg
     5641 gcagttcagt cgggcgagca gaaataatcc atggacgccc gcatataact gcgttacaat
     5701 tacagccaat cgaacaccta tgtcaaaagt attgataatc gaggatgata ttttccttac
     5761 tgaagtattg gaaaagaaaa tgaatgatgc cggctatgca gtggaaattg cccgtgacgg
     5821 cggagtcgga ttgacaaaac tgaaacaaga caagcctgac attgttttgc tagatcttgc
     5881 gcttccggta aaaggggggt ttgagatttt ggaagaaaaa atgagggatg cttcaattcg
     5941 aagtattcca gttatcgtta tctcaaattc tgggcagcca cttgaaatag atcgtgtact
     6001 tgcactcaat gtcaaagatt atttcgttaa ggcccaattt gacccagatg aagttctcgc
     6061 taaagtgaaa ttgcagcttc gtgacatgga cgctgttgcg tcgcttgggg atgatgagaa
     6121 aaagactgaa aaagaacata cggcgagtct cgccagtaaa aaaataatgt gggtggagga
     6181 tgatgttttc ctttcagata tcattgcccg gaaactttca aacgagggtt caaaattgat
     6241 tcacgcggcg aatggaaacg atgtgtttga tatggcaaag aaagaaaagc cggacgttat
     6301 attacttgac atattgctcc cggggaaaga cggttttcac gtgctcgagc agttaaaggc
     6361 cgtatccgag acaaaagaaa ttcccgtgat attgctttca aaccttggac agaaaagcga
     6421 catcgaaaaa ggtgaaaaac ttggcgcggc ccgttttctg gtaaaggcaa ccgtgacgct
     6481 tgatgagatt gttgacgaga taaaaaagtt tgcgaaagtt tcaaagtaga ttttgaaact
     6541 gacgggtgat atagtgtctc tgtaattcct gtgcccccat gcacaggcgt ttgcgcatga
     6601 gcgggaatga tcaatttaca attatcaatt atcaagttga gcagttttga aaattgataa
     6661 ttgagactcg aaaattaatt gaatgcctaa agaaaaagaa aaaaaagatg atttgattat
     6721 tgtcaaagga gctagaacac acaatctaaa aaatgtcacg gtcgagatgc cgcgtaataa
     6781 aatgatcgtt ttcacggggc tttcaggatc aggaaagtcg tctctggctt ttgacactat
     6841 ttttgctgaa ggccagcgcc gctacgtgga atcactatcc gcatatgcgc gtcagtttct
     6901 tcatcaaatg caaaaaccag atgtggatga aattgtcgga ctgtcgcccg caatttcaat
     6961 tgatcaaaaa tctcgctcga acaatccacg ttcaaccgta gcgactatta ctgaaattta
     7021 tgactatttg aggattcttt atgcacgcat tggtaggccg cactgtcttg tgtgcaatcg
     7081 cgagatcaaa aggctttccc atgaggaaat attggagacg attatcgaga ccgtgcaggc
     7141 aactgatatt tcaaaagcgg caaaggatgt actcggagta aaaatcagtt ctctcgagtt
     7201 aaaaatctat tctcctgtcg tggtgggcag aaaaggggaa tactaccaac tgctctatga
     7261 tcttttagac aaaggctact ctgaagtaaa agtcgacgga cgggtaaaaa aacttcggga
     7321 gcagattatt ctcgaaaaaa ataagaaaca tgacatagac gtgctcgtag ataaattttt
     7381 tgttaccgaa tttaccgatg aaaagagcgg ttcggcccat tcgacaagct caaggccggc
     7441 aggttcgcca caagcaggct ggcgggagcg gctctctgaa gctatcgagc gggcgcttca
     7501 cgagtctaac ggacttatca aaatagaaac accgaatatt tcaaggtcgg accttgaaac
     7561 aaaggacaag ccaaggccag gccttggtga taaccaaggc ctggccttgg cggaaaatga
     7621 cgagtcacat gaaaggttga tttcttcgaa attcatgtgc ccatatgacg gtttctcata
     7681 tccggaaata gagcctcggc ttttttcatt taactcgccg tatggcgcgt gtctcgaatg
     7741 caacggtctc ggaacaaaat ttttcttcgg ggaagaagtg tgtccgaaat gcaacggagc
     7801 gcgtctgcgg gaagaggcgc tccacgttct tgtcggggga aaaaatattg tcgaactggt
     7861 gcgactttcg attgaggaaa caaatcgatt ttttagtcac gtcaaactga ctgccgtcga
     7921 aataaatatt tcaaaagtcg tgttaaagga aatcgaatcg agactcactt tcatgatcaa
     7981 cgtcggcatt gaatacctct cgcttgatcg ccgcgcgcac acgctttctg gcggagaagc
     8041 acagcgcatc agactcgcgt cgcaactcgg atcaggtctt gtcggcgcac tttatgtact
     8101 tgatgagccg actattgggc tccacgcacg ggacaatgat cgactgatca agacgcttct
     8161 caatttacgc gatattggta acactatcat agtggtagag cacgatgagg atacgatctt
     8221 ttcctcggat tatctcgttg atatcgggcc aggagccggt actcatggcg ggaaaattgt
     8281 ggttgcagac tatctcgacc cgctcatcac tgctcgaaaa aacgaatcta aatctttgac
     8341 actcgattat ttgcggggcg ataaaaagat tgaatttcca gccgagcggc gaacagaaga
     8401 acggggaaag attctcatcc ggggcggaag tattttcaat atcaaggatt taaatgcaga
     8461 gattccgctt ggaaggcttg tcgcgttgac tggagtttct ggctccggta aatcttcttt
     8521 tatgtatgag attgtctata aaaatctgca ggcccgcctt gaacgaaaat atcgaagcaa
     8581 taaaatctgg aattgcacat catttgcggg tacggaatat ataagcagag tcattcttat
     8641 tgatcagtca ccgatcggga ggacgccgcg ctcaaacccg gccacctaca caggcgcttt
     8701 tacgcatatt cgagatatgt ttgcgagctc cgaagaggcg cgcgctcgcg gctggaaggc
     8761 aaatcggttc tcattcaatg ttaaaggtgg acgatgcgaa gcatgccagg gaaacggaga
     8821 aattgccgtc gagatgcatt ttttacccac cgtatatgtg ccatgcgaag tatgccaaag
     8881 caaacgcttc atgaaggaga ctcttgaagt aaagtacaag aaaaaaaaca tttacgatat
     8941 cttaaagatg accattgaag aggcgatgga ttttttcaag gatattcctg cgatcagcga
     9001 tagattggaa acgctcaatg aagttggtct cggctatcta gagctcggcc agtccgcgac
     9061 gactctctcg ggcggagaag cacagcgagt taaaatttcc tccgaactat accggcccca
     9121 tgtccaaaag actatctact tacttgatga gccgtcaatt ggcctgcatt atgaagacgt
     9181 tagaaaattg attgagattc tcgaaaaact ggtcaaaaaa ggaaatacgg tcatgctgat
     9241 cgagcacaac atggacatca tcaaatgcgc cgattgggta attgatatcg gacccgaggg
     9301 tggggacgcg ggcggtaaga ttgtggcgaa aggaactccc gagcaggtgg caaacgtcgc
     9361 aaaatcttac acagggcact atttgaagaa aatattaaag agagcttaat tcatctatcc
     9421 gctgtcgcat aatgcgttat acgattcgat acaacgcgcc caaaaggcga tttggcaaca
     9481 tacattaaaa ttacgcacgt gcgtaatttt aatgtatgtc gtatattgtg taagatatgt
     9541 ttttgaaatt gttgagtcgc tttgtgtgat tcatacgttt cgtcgaacaa caaacggact
     9601 cctttgcttt tgtccctctc ttacaactat atgtgcgtag tcacatatat gatcacttcg
     9661 tctcatataa tgacgtcact cggacataat gtaagactcc gtgttccatt tgctccttgc
     9721 ttgtcattat aattgctgcg ttcggtccaa aaactagagg aagacctttg cttttgtccc
     9781 tctcttgcaa ttataataaa ctcaatggaa tttacgttct ccttagctca tatatctata
     9841 ttggcaggat cagtcattgc acttatcgtc gcgagttttt ttgcgttgag tgttgttagg
     9901 aggaaaatgg cgtcatccgt aaatgcgggg caaaaaaaca aaattgatac tattaaatac
     9961 gaatttgtta ccgtcatcac ccataaattt agaacaccac ttacacaaat aaagtggggg
    10021 gcggaaggat tgcgtgccgc cgccacggag acgcaaaaag aaattgaatt ccggaaaatt
    10081 gaacgcgccg cagacacaat catcgcgatg actgaggtgc ttctcaaagt agctgacgcg
    10141 gatacatcgg gaatcgaact tcaactcgaa ccggttggtg tcggcgaatt tgtgaggtct
    10201 gtggcaaatg aattgtcgtc tgactttatg ctgaaaaaag tcacgccgga gtttacacag
    10261 ctttcttcaa ccccgtcgct tgtccgaatt gataagaaac aaatgcgcct cgtctttctg
    10321 acgataatgc agaatgcggc gacctatgtt cagccagccg gtgccgtcca tgtcactttt
    10381 agagaggaac ggggtttttc aataactgag gtttttgaca atggagtggg aattccgaaa
    10441 gatgaattgg ccagagtgtt tgatcgtttt taccgaggtg cggtggcacg tgaaattgat
    10501 caggacggac ttgggatcgg actctacatg tctcggaaaa taataaattt gcacaatgga
    10561 agtctcactc tcttttctga aggaaaaggg aagggaaccg tggctcttat cgcgcttccg
    10621 ctcgctccgt tgcctgaatc tgcagctggg gccgcggcgt ccgccccaat tgcttccccg
    10681 atttctgtcc tagtaccgcc taaaaatact cgagttgctg aaacagtctc tcactaataa
    10741 tcaataataa aaaaaccgat ctgtcatttt gcagtcggtt agtgagagat tgttccgatt
    10801 cggctcgtag ctacgtgcac acagatgagg aacaaaatga aagagcttga agcccacagc
    10861 gccaggtatc cttcgtggtg aaaaggatct ccactgtagg caatttttgc aaacactacc
    10921 atcgtgattg atccgatctt aattgttcct gtaatcgtaa cagccgcaat aattaaccat
    10981 gtagcgcaac gtgagagggt aatgcttttc acgtggacga tcttcgtgag agtaaggaag
    11041 tcgttaaatg gtgtcagcag ttttcgaatt gccgtgaagg tgatggttcc tagcagggct
    11101 ccaagaaatg aagctattgc tggcccaacc cgataacggt ccctgtcatc attgtgcatg
    11161 aggtgattgt ccataacatt aagacggtag attgtttatg ttgtcccaca caaaatggta
    11221 ttctcgaaat gattgggcgt caacctgatg caaaactaag cctacaaata atcaatcgtc
    11281 aaatagcaga taagtagtcg tcgagtaggg taagagtcac caaaaaacat gacatacatt
    11341 aattttacgc atgtgcgtaa aattaatgta tgtctacaaa tttattttgg ctagtgggaa
    11401 tcgagtgtcc gctggacgcg gacacagcgt ctataacgtt tcgaattaga attactgtta
    11461 ttataattcg acggtcgtag aattaaaata acaagtggaa gtcggaaaaa gtcgaataaa
    11521 aagaaaaaac caacgagtga tcgttggagt aacaagtctt cgctatagat cgtcacgtcc
    11581 atctggcttg atcggtgggg gatcggcgag ggtggcgaga aattcctcgg ccgtcgacat
    11641 taggactgag agcttccgaa catgatctga catgttcgtc tggtaagagc cctcgcttct
    11701 ttccgtggca tagatttgct tatcgaggtt gcgggctagc gcacaagcag tttctgtgag
    11761 gtagccatcc cttaccattt gaccgtgcat cttgagatca cgaagcttgg aaagtagtct
    11821 cgaggcaaag gtgaacgaga aaccgttcac tacatcggtc ggttggaacg aaagtgcgag
    11881 cgattggcgt agagcgggaa tttcagaatt cataattata gtgctggttc aaaaaatatg
    11941 ctacattact atttatgacc agtcaagaga tgctaaaaaa gaaactacca gacgttcccg
    12001 gcgtctattt tttcaaatgt gctggtgaga tcgtctacat cggtcgcgcg acttcgttgc
    12061 gcgaccggct aaagagttat ttcaaagacg acgtgataaa gacacgcgga ccacttatcg
    12121 tggatatggt aacgcgcgcg gatgagcttg acttcataac gacggaatcg gtgctcgatg
    12181 caattattct tgaggcaaat ctcatcaaaa aatatcagcc tcatgcaaat gctcgcgaaa
    12241 aagatgataa gagttttaac tatgtcgtga tcacggatga aaaatacccg agagtagaac
    12301 taatgcgctc gaaagatctg gagtctccgc taagagctga aggctatcct tcgacaggcc
    12361 gagggtcccg agtgaagtcg aaggataagc taaaagctgt ttttggtccg tatccgcagg
    12421 gttttattct taaggaaggt ctgaagatta ttagaagagt ttttccatac agagatcgga
    12481 aatgcgtacc cgaaagtgga caaccttgtt tcaataggca aatcggcgtt tgtcccggca
    12541 catgtactcg agagatttca aaaaaggact ataatcgaac cattttgcat ttgaaattat
    12601 tttttgaggg aaagaaaggt gagctcttga aaaaactgga gcgagagatg aaagagtttg
    12661 caaaaaaaag agaattcgaa aaagccaacg cgatgaaaaa gacgactttt gcccttacgc
    12721 acattcaaga cctaacgctt attaaggctg aaagtcgaga gacaccccta ttgtcaaggg
    12781 ccgcccttga c
//
LOCUS       DATDSU010000148         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2316745, whole genome shotgun sequence.
ACCESSION   DATDSU010000148 DATDSU010000000
VERSION     DATDSU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316745"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1442)
                     /gene="tgt"
                     /locus_tag="VI981_05150"
     CDS             complement(201..1442)
                     /gene="tgt"
                     /locus_tag="VI981_05150"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY4479710.1"
                     /translation="MKFTVEKKLKGTLARAGKISTLHGIIDTPAFITVGTKATAKALT
                     PRQLEEIGVQAVLANTYHLYLEPGEKIIKSAGGLGNFMNWNGPTMTDSGGFQVFSLGA
                     DFNKKKGKILTRSVDLEVGLQSQARVDEFDAKTPYSEENEDRGRLAKIDEDGVTFKSY
                     KDGGAHRFTPERSIEIQHAIGADIIFAFDECTTPIAPHQYQKRAMERTHRWAARSLLF
                     HKKNKRQDQVLFGIVQGGRFEDLRKESACVIGSMNFDGYGIGGSFEKEDMGKAVRFVN
                     ELLPLEKPRHLLGIGEPVDLFLAVENGCDTFDCVAPTRMARNGTLYTPNGRLNIQNAK
                     CVNEFVPVDSECGCYTCKNFTRAYLSHLFRSKEILGATLASIHNVYFIFHLVRRMREA
                     IIDGTFSHFKIEFVSKYEGWN"
     gene            complement(1635..1964)
                     /locus_tag="VI981_05155"
     CDS             complement(1635..1964)
                     /locus_tag="VI981_05155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY4479711.1"
                     /translation="MESKELIIEDSVVGTGAEALAGKTVRVHYTGTLTNGTKFDSSLD
                     RGEPIEFVLGTGYVIQGWEQGILGMKVGGKRHLVIPPELGYGARAVGPIPANSTLVFD
                     VELIAVK"
     gene            2158..2781
                     /locus_tag="VI981_05160"
     CDS             2158..2781
                     /locus_tag="VI981_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY4479712.1"
                     /translation="MTEKKTTPQGPKTRLNKYAAMTFGVSRRKADELIVSGTVLVNGK
                     AAILGQKVGGHDIVKLKHAPLIKHEYYLANKPSGVSVDNFLIARAKKGPALRAIDPLD
                     TASSGAQLYTSDTLFERKNTASMSAYEVQLNRHFSPSFIKIFSKSSGVKEVAPIDDTH
                     FMLRARLSTKDIKSISNALGFQVTGIHRTSSRGYDLNSMGASTFTRY"
BASE COUNT          914 a          817 c          767 g          919 t
ORIGIN      
        1 ccaaagtacg caggcaaaac gaaacgagcc cccgaagagg gctcgtttct aggattgtac
       61 gttagttttt acgcaagatc acaaaaacca gctctcttcg agagtgctag cttgataaag
      121 acggcggcgt gtaactgcgg aatgatcata tgttcatgct atagcgcatg aggtgtgaag
      181 tcaaatccgg tgtccacaga ctaattccat ccctcatatt tgctcacaaa ttctatttta
      241 aaatgactga acgttccatc aatgattgct tctctcatcc ttctgaccag atgaaaaata
      301 aaataaacat tgtgaatgga ggcgagcgtt gctcctaata tttcttttga ccgaaagaga
      361 tgcgaaagat atgcacgtgt gaagttttta cacgtataac acccgcactc actgtccaca
      421 ggcacaaatt catttacaca cttagcattt tgaatattga gtcgtccgtt cggtgtgtag
      481 agcgtgccat tacgggccat acgcgtcggc gccacacagt caaaggtatc gcagccgttt
      541 tcaacagcga gaaaaaggtc aactggctcg cctatcccta aaagatgccg cggtttttca
      601 agcggtagaa gttcattcac aaagcgtacg gctttgccca tatcttcttt ttcaaacgat
      661 cctcctattc catatccgtc aaaattcata gaaccgatca cacacgcgct ctcttttcga
      721 agatcctcaa acctcccgcc ttgaacgatg ccaaagagca cttgatcttg tcgcttattc
      781 tttttatgga agaggagcga tctcgctgcc cagcggtgag ttctttccat ggcccttttt
      841 tgatattgat ggggggctat gggagtggta cattcgtcaa aagcaaaaat aatgtctgcg
      901 ccaatcgcgt gctggatttc aatagaacgc tcgggagtaa atcgatgagc accaccatct
      961 ttatatgatt taaaggtgac gccgtcttca tcgatcttcg caagccggcc tcgatcctcg
     1021 ttttcttcgc tgtatggagt tttagcgtca aactcatcga cacgcgcctg gctttgaagt
     1081 cctacctcca agtcgacact ccgagtcaga attttaccct ttttcttatt gaagtcagca
     1141 ccaagtgaga aaacttgaaa gcctccggaa tccgtcattg tcggaccatt ccagttcata
     1201 aaattcccca gtccaccggc tgatttaatt attttttctc cgggctcgag gtagagatgg
     1261 taggtgttcg ccagtactgc ctgaactcca atttcttcta attgtctagg cgttagcgct
     1321 ttagctgtgg ccttggttcc aacagtaata aaagcgggcg tatctataat gccgtgtaaa
     1381 gttgatattt tgcccgctct cgcaagtgtg ccttttaact tcttttcgac ggtgaatttc
     1441 attctgtcag gatatccaat tgtgccactc gaagcaatga ggcttcattt acactataag
     1501 cgagccgagc attagacact agaatggtgc ttcggcaagc gcgacgccat tcttctctct
     1561 tccgacggag cgagaataca tcttttttgc gggttagcgg agcgcttaag caaaaaaaga
     1621 tgtattcgag cgccctattt taccgcgata agttcaacgt caaaaacgag agtcgagttt
     1681 gcggggatcg gaccgactgc acgcgccccg tagccgagtt caggaggaat gacgaggtgc
     1741 cgtttaccgc caaccttcat accgagaata ccctgttccc aaccctgaat aacataacca
     1801 gttcccagca caaactcgat cggttctccg cgatcaagcg agctgtcgaa tttggtgcca
     1861 tttgtaagcg tacctgtata atggacgcgt accgtcttgc ccgcgagtgc ttccgcccca
     1921 gttcccacaa cgctgtcttc gattattaat tctttgcttt ccatagtagt atcattttga
     1981 acaatcggca ccgtgttggt attcgtattt gacgaagtat cctcgacttt caccgaccgg
     2041 ttagtaagca aaccgcccat aaaatataaa accgccaaga ccgccacagc gattccaact
     2101 atctcccctt tgtttaaatt tttcatattt gtatagttaa taggaatatt gtatctaatg
     2161 acagagaaaa aaactacacc ccaaggaccc aaaacacgtt tgaataaata tgccgctatg
     2221 acattcggag tttcgcgtag aaaagctgac gagctaattg tctccggtac cgtccttgtc
     2281 aacggcaaag cggccatact tggccagaaa gtagggggcc acgatatagt aaagctcaaa
     2341 cacgcaccgc ttattaaaca tgagtactac cttgcgaata aaccctctgg cgtctcggtg
     2401 gacaattttt taattgctcg agcaaaaaaa ggccctgcgt tgcgtgcaat cgacccgtta
     2461 gataccgctt cttcaggtgc acaactttat acttcagata ctttgtttga aagaaagaac
     2521 accgcgagta tgagtgctta tgaagttcag ctcaatcgtc atttttcacc aagttttatt
     2581 aaaattttct ctaaatcctc gggggtaaag gaagttgcgc cgattgatga cacgcatttt
     2641 atgttacgag caaggctttc gacaaaagac ataaaatcaa taagcaacgc actggggttc
     2701 caggtaactg gaattcaccg gacgagtagt cgcggttatg atctaaattc tatgggtgcc
     2761 tccactttta ctcgttacta gaatttgacg tatggcgcat taggacaccg tgcgactgca
     2821 acttttcatg ggggtgttca cgtaattgct cctcaaacac atcgttaggt cgaaagaagg
     2881 taaccttatg atgcttaggt aggtggtgaa ccaattcttg ggggctaaag ggagacgaat
     2941 cagcttgcgt cctgtgttct cctactttat tgcctaaact atgtcgtttt atgtagtgcc
     3001 agagcctacg agcaaacaac ataaaaacaa ccaaaacaac tacacctgcc aaaagaacta
     3061 gatagatttc tttatccaca ataatagaat tatacaaaaa ttgtgttgcg cgaaaaaggc
     3121 agaaacgaat tgaggcgcga cgctcggtga gcatcgcgcc tcggattacc agtcattggt
     3181 gggacgtaac tacgtaaacg gtttgttcat gttggtcctt tcaggccctg ccgcggaagt
     3241 gcggctacga accggccacg gcggaggcga tgtccaaatt gccggcggcg gaggatgtga
     3301 ccgaataata atcggccggc aaatgatgac acatggccca cgagccgacg gcggcggagg
     3361 ttgaggaggg gcatttcagg gcttttttcg tcacgttggt gctgttgggc ggactct
//
LOCUS       DATDSU010000149         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2474947, whole genome shotgun sequence.
ACCESSION   DATDSU010000149 DATDSU010000000
VERSION     DATDSU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474947"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VI981_05165"
     CDS             <1..205
                     /locus_tag="VI981_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479713.1"
                     /translation="VISNVVKIAASRTNNFAEFDRMTISVTNSAGVISQTNVAFFTQP
                     SARITSTPLGWEKSRHMGWVIAP"
     gene            complement(259..1500)
                     /locus_tag="VI981_05170"
     CDS             complement(259..1500)
                     /locus_tag="VI981_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY4479714.1"
                     /translation="MPLFKLRSKKIAHEEISAPDITPEKVGETDVAPPQKEAPLKVRD
                     TKEADEKEAAKKMSTMGVLLERAGTPPGLGDLVEGVVINIDKSGVYIDLSPFGTGIIY
                     GREYINARDIIKKINLGDTIAAKVVDIDNVEGYVELSLKEARQALIWSDAEAAIREKR
                     VLELSVREANKGGLIIEWQGIIGFLPASQLKTEHYPRVADGDKDKILEELKKLVGQKI
                     AVSIISAIPKEGKLIFSEKNPEQKDKGKIIEKYTVGDTLEGEVTGIVDFGVFVKIEEG
                     LEGLVHISEIDWALVEDPKALFKVRDKVMVKVIEIKDGKISLSIKALKENPWIEATKK
                     YKKDDIVSGVVIKYNKHGALASIEEGVAGLVHISEFGGEDKLREKLELGKTYKFKITL
                     FDPKEQKMALSFTGAEKNLVV"
     gene            1815..2450
                     /locus_tag="VI981_05175"
     CDS             1815..2450
                     /locus_tag="VI981_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4479715.1"
                     /translation="MVITYLGAEFFKVQYGDTTIAYNPVSKESKLKSARFGADIALVA
                     LNHRDMTGAGQVEFGGKKPFVISGPGEYEIKGIFTKGYKSLSHYDGEERYNTIYVLNL
                     ENMNLCFLGALSKDLDSETKEALDDIDILFLPIGGKGVLTPAEANKLAVSLEPKLVIP
                     MHYGDIGEQNALKTFLKEAGENPKPIDKLTIKKKDLEGKEGEVVVLEPAGA"
     gene            2467..>2757
                     /locus_tag="VI981_05180"
     CDS             2467..>2757
                     /locus_tag="VI981_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479716.1"
                     /translation="MAINFLDKLRSKSDGTKRVIALFASLALTFVIAIVWLSSLYIQA
                     QLADPENAKYEEQSPFKTIVDGAKANLRWLEKGSTTTNTGPAIIEGDGFTNDA"
BASE COUNT          738 a          623 c          592 g          804 t
ORIGIN      
        1 tgtaattagc aatgtcgtta aaattgcggc cagccgcaca aataacttcg cggagttcga
       61 ccggatgaca atttcggtca cgaacagtgc cggagttatt tcacagacaa acgttgcgtt
      121 ttttacccaa ccgtctgccc gcattacaag cacgccgctc ggttgggaaa agagccgaca
      181 tatgggctgg gtaattgctc cctgatttga ccgtctgagt cccacatcgc cgtgcacaaa
      241 atttgtgctc ggcttttttt atacaacgag gtttttctcg gcaccagtga atgaaagcgc
      301 cattttttgc tctttaggat caaagagggt gattttgaat ttgtaggtct tgccaagttc
      361 gagcttttct cgaagtttgt cttcccctcc gaattcagaa atgtgaacga gtccagcaac
      421 gccttcttcg atagaagcga gcgcaccatg cttgttgtat ttgatgacaa cacctgaaac
      481 gatgtcatct tttttgtatt tctttgtcgc ttcaatccag ggattttctt tgagcgcttt
      541 tatggaaagt gaaatctttc cgtcctttat ttcaataact ttaaccataa ctttatcgcg
      601 taccttgaaa agcgctttcg gatcttcaac aagcgcccag tcgatttcag aaatgtgtac
      661 gagtccctca agcccttctt caattttcac gaagacgccg aagtcgacaa tacccgtaac
      721 ttctccctca agcgtgtctc cgacagtata tttttcgatg atttttccct tatctttttg
      781 ttccggattt ttctcggaga aaatgagctt tccttctttt ggaattgcgg aaataattga
      841 aactgctatt ttctgtccga ccaatttctt gagttcttca agaattttat ccttatctcc
      901 atcagcgacg cgtggatagt gctcggtttt aagctgcgag gcaggtaaga atccgatgat
      961 gccttgccac tcgataatca gtcctccttt gtttgcttca cgcacagaaa gttcgagcac
     1021 acgtttttcg cggattgccg cttcagcatc gctccagatg agcgcttgcc gcgcttcctt
     1081 gagcgagagt tccacataac cttcaacgtt gtctatgtcg acaactttag cggcaatcgt
     1141 gtcgcctaga tttatttttt tgataatatc gcgcgcattt atgtattctc gtccgtaaat
     1201 aatacctgtg ccaaaggggg agaggtcaat atacacgcct gatttatcga tgttgatgac
     1261 tacaccttca accaaatcac ctagacccgg aggagtgcct gcacgttcaa gaagcacgcc
     1321 catggtgctc atcttctttg ccgcttcttt ctcgtcggct tcttttgtgt ctcttacttt
     1381 gagaggcgcc tctttttggg gaggagcaac atccgtttcc cctacttttt ccggagttat
     1441 atccggcgcg gaaatttctt catgcgcgat cttttttgaa cgcaatttaa ataatggcat
     1501 tgttttttag aaggtccgcg cacgccgatt attgtaaggt ggcacacagg gatagcttca
     1561 gggccatgtt tcgcacgagg gcgaaatcat gcccgaactc aatatgagat tcaaccacta
     1621 tactcgaaac ctttcgaaat gcaactttcg cgttttgagt caaaaagttt aggtttaata
     1681 cagagctcaa aataacgtat acgagtgaac tatatagcat atatttagtt cactcagcaa
     1741 aatcataaaa aacgccgttt tttcggtgct cgcaactaga atgtctaaat tctctctgct
     1801 atactgtttc aaccatggta attacatatt taggggctga attttttaaa gtccagtatg
     1861 gcgatacaac tatcgcctat aatcctgtat caaaagaatc aaaattaaaa tctgcgcgtt
     1921 ttggtgcaga catcgctctc gtggcgctta accacagaga tatgacgggc gcagggcagg
     1981 tagaattcgg cggcaaaaaa ccgtttgtaa tttcaggtcc tggagagtat gaaattaaag
     2041 gaatttttac aaaaggctac aaatctcttt ctcattatga tggcgaagag cgatacaata
     2101 ctatctatgt tcttaatttg gaaaacatga atctctgttt cctcggcgcg ctttcaaaag
     2161 atttagattc tgaaaccaaa gaggcacttg atgatattga tattcttttt ctgccgatcg
     2221 gcggcaaagg agtgttaacg cctgccgaag ccaacaagct agccgtatcg ctcgagccta
     2281 aactcgttat tccgatgcac tacggagaca ttggcgaaca aaacgcgtta aaaacctttc
     2341 tcaaagaagc gggggaaaat ccgaaaccga tcgataaact gacgatcaag aagaaagatc
     2401 tcgaaggcaa ggagggagaa gtagtagtgc tggagcctgc gggagcgtaa agcgcgtgag
     2461 ccaaatatgg cgattaactt tctcgataaa cttagaagca aatccgatgg aacaaaacgg
     2521 gtcatcgcgc tttttgcgag ccttgcactt acatttgtga ttgcaattgt ctggctttca
     2581 tcgctctata tacaagcaca acttgctgat ccggaaaacg cgaagtacga agaacaatca
     2641 ccctttaaga caatagtaga tggagcaaag gcaaatctcc gttggcttga gaagggttcg
     2701 actactacca atacaggtcc ggctattatc gaaggagacg gattcacaaa tgacgca
//
LOCUS       DATDSU010000150         7137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1664594, whole genome shotgun sequence.
ACCESSION   DATDSU010000150 DATDSU010000000
VERSION     DATDSU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664594"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VI981_05185"
     CDS             complement(<1..800)
                     /locus_tag="VI981_05185"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEY4479717.1"
                     /translation="MIRVVDIRIRKSQRVVYVPRRVVRTSRGVFRVFLSVQKQMTAGA
                     LAVVIILTQASAFIAFEAHIVNVTAELVQIDPPVITPDSLTFNTPFDVSIDDADPDAT
                     HIFYSIGAGSLDPDTVPDPACGDPNGGPKPVGPIPVGAVDTVIKAIACDGPSAAAHGS
                     LIVYEIYDFVQPYGTIQGHKYHDLDQDAIFDIGLDFPIQGWQVTIFDGGATTTTVTDP
                     NGFYEFTNLIPGVYTIREESRDGWVHVTPKQYSITIDSTETETVDFYN"
     gene            complement(930..1352)
                     /locus_tag="VI981_05190"
     CDS             complement(930..1352)
                     /locus_tag="VI981_05190"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY4479718.1"
                     /translation="MRRLFKFLLNVSIYIAIVVALLYGLPRFLSWYLGTPYPIAAITS
                     GSMWPALHIGDLVFIQRVAKADIKVGDIVVWQNPNGFTIHRVKELRENDLVTKGDANF
                     TDDPAVKYEDVVGRTYQIWGKNARLPYFGMITVYASKR"
     gene            complement(1559..1876)
                     /locus_tag="VI981_05195"
     CDS             complement(1559..1876)
                     /locus_tag="VI981_05195"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY4479719.1"
                     /translation="MQFTISNQDSMTQLRKYDLEERTALFGELIIKLCKSLPQNTITK
                     PLIHQLIRSCTSIGANYMEANGASSRKDFRNKIFICKKETQETKHWLRMLAEGGCLKS
                     RQN"
     gene            complement(1886..2401)
                     /locus_tag="VI981_05200"
     CDS             complement(1886..2401)
                     /locus_tag="VI981_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479720.1"
                     /translation="MFGRKKDESPPNVDPMIFPRHEGSSWWERRTGEKLKLWEKIAGM
                     LFLLVFLFVFYITLDANKYRAEVRVIEGEGKVGVNPTTESLDFGDLSRGTSAIRTVTI
                     KNDSFTPLFVSIVQLGGISDLVKLNRNNFRLAKGEEQKIEFTLYMPASAEIDKQYTGR
                     VFIFKIPTLGL"
     gene            complement(2706..3530)
                     /locus_tag="VI981_05205"
     CDS             complement(2706..3530)
                     /locus_tag="VI981_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479721.1"
                     /translation="MKKKIVIGLAALGTAVALVPLFAAFEAHVINVTAKIENALLVPV
                     DPIAFGTVFPQEQLERTLPISLSQSFLDEDRVDDVEYIIRQKPKCGVVHEEDGTVNPS
                     WTGHVVVVPVHDAAGQIIGYRYSVDCEKERPINVPSNEQNPEFYLLPSLCQYLSKHSE
                     RVGSDTNGPIYEDGSLASFHQPFTIDQNNGAILWNDVGGRLAKSVNDILDTWTIDLKV
                     PCFGGYCAQDWEKYVTDINPNANPAEYTQPIGNEHKVFGCNLWVEVKGVSETPIED"
     gene            complement(3644..3901)
                     /locus_tag="VI981_05210"
     CDS             complement(3644..3901)
                     /locus_tag="VI981_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY4479722.1"
                     /translation="MFYVYILRSKRDRGLYIGYTNDLRRRVKEHNSGSTRSTKARKPF
                     ELVYYEAYKSQRDAKHREKALKLFGRARESLKRRLIDTLKS"
     gene            complement(4041..5366)
                     /locus_tag="VI981_05215"
     CDS             complement(4041..5366)
                     /locus_tag="VI981_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY4479723.1"
                     /translation="MRKKIFLGFCALSTAVALVPLFAAFEAHVINVTATIENALNVPL
                     NELTFGTVFPQEKLDQSFDVSLSQSFQGEGRVDDVTYVIRQKPKCGVLEPGTDPVAYS
                     AFLQVTDASDGTFVCPEGSVELPLLCPYMSKHEITTDGQVLQNDPKPDLGPFHGPISD
                     WTLQHSLVFQVAGKLIKSAGDLSDTWNIDLKVPCFTGNCAQDWEKFVHDNGEDGDETP
                     ADAYIQPKDREHQLFGCDLWLEVTGISLPGLGCNEQLDLMLVLDRSGSIDSGELTTLK
                     NAANAFITALAPSTAGVHTGQSSFSTAGTLDLHLTDDATAAHAAVNALVSSGRTNLYQ
                     GISLAKGELDDLHVPHERPGVKDVMIIITDGNPNEPTSEAIARALAKSEADVARVAGI
                     EIFVVGIGGDVDAAYLQTIADDPAHYFAAANFSDLQAILTGLTQCNPTP"
     gene            complement(5993..>7137)
                     /locus_tag="VI981_05220"
     CDS             complement(5993..>7137)
                     /locus_tag="VI981_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY4479724.1"
                     /translation="KVINMSLGGPAPATYLEDALAYAYNKGVTIVAASGNDNAGSVSY
                     PAAYNQYVIAVGATRFDETRAPYSNYGSALDIVAPGGDLGVDQNHDGYGDGVLQQTFG
                     NNIGSFSYYFYSGTSMATPHVAGVAALIISAGKASTPADVQTLLQNTADDLGPTGRDD
                     QYGWGIVDAAEAVAGGSIPPPPPPPPAQCADGIDNDGDSLVDLADPGCTSSTDNDETD
                     PIPPPPGPIILFTDDFQSGFAKWTESNEFDWNPESPAERAVPGSVSGNRVAHADACRL
                     TAGCFLTTTNTIDMSAYSSASLSFKRYVDNALDSGEFLRVDIFDGSTWRQVAYWTNGA
                     GDDDTWRTETIDLTPYRSANFKVRFVSKENSTTEEVEVDDVVVTAN"
BASE COUNT         1916 a         1705 c         1579 g         1937 t
ORIGIN      
        1 aaattataga aatctacggt ttcggtttcc gtcgaatcaa ttgtaatcga atactgtttg
       61 ggtgttacgt gtacccaccc atcgcggctt tcttcgcgaa tagtatagac gcccggtatt
      121 agattggtaa attcgtaaaa cccattcgga tcagttacag tcgtcgtcgt tgccccgccg
      181 tcaaaaatcg tcacctgcca cccctgaatc gggaaatcga gtccaatatc aaaaattgcg
      241 tcttgatcga gatcgtgata tttatgcccc tgaattgttc cgtatggttg tacgaaatca
      301 tagatttcgt agactataag gcttccatgc gctgcagcac tcgggccatc gcatgcgatt
      361 gctttgatca cggtatcaac ggcgccgaca ggaatcggcc ccacgggctt tggcccgcca
      421 ttggggtcgc cgcatgcggg atcaggcacc gtatctggat caagcgagcc cgcgccgatt
      481 gaataaaaaa tatgcgtcgc gtcgggatct gcatcatcga ttgatacgtc aaatggagta
      541 ttgaaagtaa gactgtctgg cgtgatgacc ggcggatcta tttgtacaag ctctgccgtt
      601 acattcacaa tatgcgcctc gaatgcgata aacgcgcttg cctgcgtcaa tataatgaca
      661 acagcaagcg cccctgctgt catctgtttt tgcaccgaca gaaagacacg aaaaacaccg
      721 cgcgaagtac gcacaacgcg gcgcggcacg tataccaccc tttgcgactt tcgtattctg
      781 atgtcgacaa cacgaatcat ataataattt tcaatttcta gttttcaatt ttcattgaat
      841 gaccagtgat ccaattttca aagtgtttta gataattgtg atattgtaaa ttgattgaaa
      901 attgaaagtt ggtcattgaa aattgctatt taacgtttac tcgcatatac cgtaatcatc
      961 ccgaaatacg gaaggcgggc gttcttgccc catatttgat atgtccggcc gaccacatct
     1021 tcatatttta cggccggatc atcggtaaaa ttcgcatcgc cctttgtaac aagatcattc
     1081 tcgcgcagtt ctttgacacg gtggattgta aaaccgtttg ggttttgcca aaccacaata
     1141 tctccgactt ttatatccgc ctttgcaacg cgctgtatga aaaccaaatc tccgatatgc
     1201 agcgcgggcc acatggatcc agacgtgatt gcggcaatcg gatacggagt ccccaagtac
     1261 cagctcaaaa atcgcggcaa gccgtacaaa agagcaacca cgatggcgat atagatactt
     1321 acattcagca aaaatttaaa taatcgacgc atacaactta aataattttc aatttctagt
     1381 tttcaatttt cattgaatga ccagtgatcc aattttcaaa gtgttttaga taattgtgat
     1441 attgtaaatt gattgaaaat tgaaagttgg tcattgaaaa tttatttagt atttccgagt
     1501 gttgtcgtaa ttttctgaaa aataagtgcg agttcttgtg cttctttcca tgcttttctc
     1561 aattctgtct gctttttagg caccccccct ctgcaagcat ccgcaaccaa tgtttcgttt
     1621 cctgcgtttc tttcttacaa ataaagattt tattgcggaa gtccttgcgc gagcttgcgc
     1681 catttgcctc catgtaattt gctccaatac ttgtacaaga acgaataagc tggtgtatga
     1741 gcggttttgt tattgtattt tgcggcagtg atttacataa ttttatgatc aattcgccaa
     1801 acagcgcggt gcgttcttcg agatcatact tgcgcaattg tgtcattgaa tcttgattgg
     1861 aaattgtaaa ttgcaaattg gaaatttaaa gaccaagcgt tggaatcttg aatataaaca
     1921 cgcgtccggt gtactgctta tctatctccg ccgatgcggg catatagagc gtaaactcta
     1981 ttttttgttc ttcgcccttg gcaaggcgga aattgtttcg gtttaatttt acaagatccg
     2041 aaatgccgcc caactggaca atcgagacaa aaagcggggt aaatgaatca tttttgattg
     2101 ttaccgtgcg gattgccgac gtcccccgcg agaggtctcc gaaatccaga ctttccgtcg
     2161 ttggattgac ccctaccttg ccttctcctt cgatcacgcg cacttccgcc cgatatttat
     2221 ttgcgtccag cgttatataa aacacgaaaa ggaaaacgag cagaaacaac attcccgcga
     2281 ttttttccca aagttttaat ttctcgccgg ttctccgctc ccaccatgag ctcccctcat
     2341 gccgaggaaa aatcatgggg tctacatttg gcggactttc gtcttttttt cttccaaaca
     2401 tgtaactaat actgcctagt gaaccccgtg taccacagct ttacagctgt aatagcaggg
     2461 cgttttacct ttctataaca aaaggtggat tgctcccccg acctcacaag ttgacttcct
     2521 gtggtacggg gtaaactagg aattacttct aagaaatttt caataatcaa tttttaattt
     2581 ccaatcccgt acacttcgtc aaggtcggac cttgacgaag tgtgggattc tatccgcctc
     2641 ttcccacccg cccccgtccg aatttcggag aaacttgggc ggatgaaggg cgagagagag
     2701 ataacctaat cttctattgg cgtttcacta acacctttga cttcaaccca gagattacaa
     2761 ccgaacacct tgtgctcatt tccgattggt tgcgtgtatt cagctggatt tgcatttgga
     2821 ttgatatcgg tcacatattt ttcccaatcc tgggcacagt aaccaccaaa gcagggtacc
     2881 ttaagatcga tggtccatgt gtccaagata tcattgacag acttggctag acgaccgccg
     2941 acatcattcc agagaattgc accattattc tgatcaattg tgaacggctg gtggaaagag
     3001 gccaaactgc catcctcata gatagggcca tttgtgtcgc tacccactct ctcggagtgc
     3061 ttggagaggt actgacaaag cgagggcaaa aggtaaaact ccggattttg ctcgtttgaa
     3121 ggaacgttta tgggtcgctc cttttcacaa tcaacagaat atctgtagcc tataatttga
     3181 ccagccgcat catgtacggg tacaacgaca acatgaccag tccaagaggg attaacggtt
     3241 ccatcttctt cgtggactac tccgcacttc ggcttttggc gaatgatata ttcgacatca
     3301 tcaacacgat cctcatctaa gaaagactgg gataaagaga taggaagagt gcgctcaagc
     3361 tgttcttgcg ggaatacagt accgaaagca atcggatcaa ctggcaccaa aagggcattc
     3421 tcaatctttg cagtcacatt gattacatgt gcttcgaatg cggcaaacaa agggaccaag
     3481 gcaactgccg ttcccaatgc cgcaagacct attacaattt ttttcttcat aagtttcctt
     3541 aatttatttc taataatttt atccccccgc actaatcaag gctcaataga ctactgataa
     3601 aaccttgatt agtggcgggg ccgacctttt tcccccacac tcatcaggac tttagagtgt
     3661 cgatcaatct gcgcttgaga ctttcacgag ccctaccaaa cagtttgagc gccttttcgc
     3721 gatgtttggc atctctctgt gatttatagg cctcgtaata cacaagttca aatggcttac
     3781 gagcttttgt tgatcttgtg ctcccagaat tatgttcttt tactcttcgg cgcaaatcgt
     3841 tcgtgtaacc aatatatagc cctctatccc gcttgctccg aagaatgtac acatagaaca
     3901 taattcaaag tcctaatgag tgtgggggtt gatattttac attttgttaa tgtaactctg
     3961 accataatct gtccgacgag acggaattat gaccagacga tcctgcaccc gtttgggatg
     4021 caggagtcgt ccaatcacaa ctatggagtt ggattacact gcgtgagacc agtgagaatt
     4081 gcttggagat cactgaagtt tgcggcggca aaataatgtg ccggatcgtc cgcaattgtc
     4141 tgcaaataag ctgcgtccac atctccacct attccgacaa caaagatttc aatgccagcg
     4201 actcgtgcta cgtccgcttc tgattttgcc aaggcacggg caatagcttc cgatgtgggt
     4261 tcatttggat tgccatcagt aataataatc atcacatctt ttacgcctgg gcgttcatgt
     4321 ggcacatgga gatcgtcaag ttcccctttt gcaagcgaaa tcccctgata caaattagtc
     4381 cggccgctac tcacaagcgc gttcactgcc gcatgagcgg ctgttgcatc gtctgtaaga
     4441 tgaagatcga gcgtacctgc cgttgagaaa ctcgactgac cggtatgcac gcctgctgtt
     4501 gagggagcga gagccgtaat aaatgcattt gcggcgttct tgagtgtcgt aagttctccc
     4561 gaatcaatac tccccgatcg atcaagaacg agcatcaagt caagctgttc gttgcaccca
     4621 agccccggca agctaatacc ggttacttca agccaaaggt cacatccaaa aagctggtgc
     4681 tcgcgatctt ttggctgaat atacgcatct gcgggagttt catcaccgtc ctctccgtta
     4741 tcatgaacaa atttttccca atcctgcgcg cagtttccag taaaacacgg gactttcagg
     4801 tcaatattcc acgtatcaga aagatcgccg gctgatttaa taagctttcc tgctacttga
     4861 aaaaccagtg aatgctggag tgtccagtcg ctaatcgggc cgtgaaatgg acctaaatca
     4921 ggttttgggt cgttttgaag tacctggcca tccgttgtta tctcgtgttt agacatgtac
     4981 gggcagagaa gcggaagctc taccgacccc tcgggacaga caaacgtgcc gtctgaagcg
     5041 tcggtaactt gcaaaaatgc tgaatatgca acgggatcgg tacctggttc aagaacaccg
     5101 cattttggct tctggcggat aacataggta acatcatcaa ccctgccttc accctgaaaa
     5161 gactgtgaaa gagaaacatc gaacgactga tcgagttttt cctgcgggaa gacagtgccg
     5221 aacgtaagct cgttcaacgg cacattgagt gcattctcaa tcgtagcggt cacgttgatc
     5281 acatgtgctt caaatgcagc aaaaagcggg acgagtgcca cggctgtcga caaagcacag
     5341 aaaccgagaa atattttttt tctcataatc tcaactatcg agattcacga atcaagattc
     5401 aaaaatcgag aggagatttc tgaatcacgc tccgtaaatc tttttattaa atttttaata
     5461 aatcccgtta gaaattccga ctaggtatta tttctaactg gataagcaga taaaactaaa
     5521 ccttatcgac gaggtctagc tcaatataaa aattttattt gccatatatg catggcttct
     5581 tttcgtcttg gttctctcca gaagacacgg gctgcacatg caaaccgtaa ctctattcat
     5641 atactcttgg gatacaggag gtcaagaaaa aatgtgcata gtctgtggaa ggcatacaag
     5701 aacataaagc tatgtattcg gcaagacatg ccggacaacg gcacggaaaa acggaatata
     5761 ttctcgtcaa acaccgatta tgaacggttt ctttccctga taaacctctg catctcaact
     5821 tcaaggttcg accttgacag aggagaagag gaatggaaga aggaagcgaa agttagggta
     5881 agtttaaact tcaaggtccg accttgaaga gttgaacgcg cgcaaaacaa aatcccgcgc
     5941 cgtagcaagt aagcgtaagg gcgcgggatt ttttatacga atcttctttt aattaattcg
     6001 cggttacgac aacatcatca acctctacct cttctgtcgt cgaattctct ttagaaacga
     6061 agcggacttt gaaattcgct gaacgatagg gagtaagatc aatcgtttct gtacgccacg
     6121 tatcatcatc ccccgctccg ttggtccagt atgcaacttg tctccacgtt gagccgtcaa
     6181 aaatatcaac acggagaaat tctcccgaat caagagcatt atctacataa cgcttgaatg
     6241 aaagcgatgc tgacgaatag gcgctcatat caatcgtgtt ggtcgttgta aggaaacacc
     6301 cggcagtcaa acggcacgcg tccgcatgcg caacgcgatt gcccgataca gatccgggga
     6361 cggctcgctc ggctggagat tcggggttcc aatcaaattc atttgactcg gtccatttgg
     6421 caaaacccga ttggaaatca tcagtaaaga gtattatcgg cccgggcgga ggcggaatgg
     6481 gatccgtttc atcattatca gtagaagagg tacagcccgg atcagcaaga tctacaaggc
     6541 tatcgccatc gttgtctatg ccatcggcgc attgggcagg cggaggtggt gggggcggcg
     6601 gaattgagcc tcctgcaaca gcttctgccg catctacaat accccagcca tattggtcgt
     6661 cccgccctgt cggacccaaa tcatcggccg tattttgcaa aagcgtctga acatccgcag
     6721 gggttgaggc ctttccggca gatatgataa gcgcggcaac accggctacg tgaggtgtcg
     6781 ccatacttgt acccgagtaa aagtaatagc tgaatgaacc gatattattt ccaaatgtct
     6841 gctggaggac accatcgcca tatccgtcat gattttggtc aacaccgaga tcgcctccgg
     6901 gcgctacgat atcgagcgcg gagccgtagt ttgaataggg cgcgcgtgtt tcatcaaaac
     6961 gcgttgcgcc gaccgcaatc acatactggt tgtatgcggc gggataggag acggaacctg
     7021 cgttgtcgtt gcctgaagcc gccacaatag taaccccttt gttatatgca tacgcaagcg
     7081 cgtcttcgag ataggtcgcg ggtgccggac cgccaagcga catattgatg actttcg
//
LOCUS       DATDSU010000151         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_324184, whole genome shotgun sequence.
ACCESSION   DATDSU010000151 DATDSU010000000
VERSION     DATDSU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324184"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..962
                     /locus_tag="VI981_05225"
     CDS             177..962
                     /locus_tag="VI981_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEY4479725.1"
                     /translation="MSQHIELGVRIARKAGEIMREHFRKDVHIDWKSEDHTPVTVVDT
                     SVNELLLKEIPLVLPDYAIYAEEKSLAKESRFAAVCDPLDGTFPFTHGIPTFAFSLAF
                     TEDGDPFACIIYDPILDRMYTAEKGKGAYVNGARISVKEWPLKNPEERLSVAISWDYG
                     QRDLSQVHASLQKYPAACFNLLSAVYMGMLVASGEIGGCIVPVKYPWDCAAIKLLVEE
                     AGGKVTDLFGSEQRYDRTVDGFVAAHPSLHQILVGMIQKNVMK"
     gene            complement(985..>2590)
                     /locus_tag="VI981_05230"
     CDS             complement(985..>2590)
                     /locus_tag="VI981_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4479726.1"
                     /translation="LGKLPFEMIQKLRRLGGLPGHPDRQTPYLMTNTGSLGMGISKAR
                     GMAKGKRLQGKTGRVYVLTGDGELQEGQIWESLQPTVNGKFSEITVIVDHNKIQSDTH
                     VKDTSDLGEIENKFKAFGWEVMRINGNDMREIKKAIEWAKSIKEKPQVIIADTLKGAG
                     VSFMTRLADDGLYKFHSGAPSYEQFEAAVKEISDRINEKLRNAGEGELELEFESTEMP
                     SSSIGQNLERLIPAYADELVKIAKENKKVVAMDADLVLDTGLIQFKKEIPERYIECGI
                     AEQDMVSMAGGLAIQGLIPIVHSFECFLTTRANEHFYNNATEKTKIVYVGSLAGILPG
                     MPGHSHQSVRGISILGSIPGLTLIQPSNEKESRMAIRWAIEENPQSTYIRFVSIQNEC
                     PFSFPKNYTLESGKGVFLREGKNVVIIGYGPVMMTEAMKAASILEEKGISASVINFPW
                     LNRIDQAWLKKVVEGYKAIVTLDDHYVALGQGMQIRSALAPHINKQKILSLGIEEIPV
                     CGHNAEVLKYHKLDFESIVEEVKKNL"
BASE COUNT          638 a          620 c          561 g          771 t
ORIGIN      
        1 atcaatctat tttggttacg tttcgcacta attaccaaac tttttgtatc gcatctcatt
       61 catttataga acatccgagc cctcaccgtt gtttcgacgc tttctttttt tcattgtatg
      121 tattgacaag aagtatacat atggtataag ggtttagaat cttaaatgag gactctatgt
      181 cacagcatat cgaacttggc gtccgtattg cccgaaaagc gggtgagatc atgcgagaac
      241 atttccgcaa agatgttcac attgattgga agagtgagga tcatactcct gttactgtcg
      301 ttgacacgag tgtgaatgag ttactattga aagaaattcc tttggtactt cccgactacg
      361 ccatctatgc ggaagaaaaa agtctcgcaa aggaaagtag attcgctgct gtttgtgatc
      421 ccttggatgg tacgttcccg tttacgcatg gaattccgac atttgcgttt tcccttgcgt
      481 ttaccgagga tggtgaccca ttcgcgtgca ttatatatga tccgatcctt gataggatgt
      541 atactgcgga aaagggaaaa ggtgcatatg taaatggcgc acggatttcc gtaaaggagt
      601 ggcccctaaa aaatccggaa gaaagactct ccgtcgctat atcatgggac tatgggcaac
      661 gagacctctc ccaagtgcat gcatcactcc aaaaatatcc tgccgcgtgc ttcaatcttc
      721 tttcggcggt ctatatggga atgctcgttg cttcaggaga aattgggggt tgcatcgttc
      781 cggtaaaata tccctgggac tgcgcggcga tcaaactctt ggttgaggaa gctggcggaa
      841 aagttactga tcttttcgga agtgaacagc gatacgatag aacagtagac ggatttgtcg
      901 cggctcatcc atccctccat caaatactcg ttggcatgat tcaaaaaaat gtgatgaaat
      961 agttcctgcg aaagcaggaa ttttttatag atttttcttg acttcctcca cgatcgactc
     1021 gaaatcgagc ttgtgatatt tcaatacttc ggcgttgtga ccacagacgg gaatttcctc
     1081 gatcccgagt gatagtattt tttgcttgtt tatatgtggc gcaagcgcgc ttcgaatctg
     1141 cattccctgg ccaagcgcta catagtgatc atcaagcgtc acaatcgctt tatatccttc
     1201 aactactttt ttaagccacg cttgatcaat ccggttgagc caaggaaaat ttatcaccga
     1261 ggcagaaatt cctttttctt cgagaatact tgccgctttc attgcctccg tcatcatgac
     1321 cggaccatat ccaataatca ctacattttt tccttctcga agaaaaaccc ccttaccaga
     1381 ttcaagtgta taattttttg gaaatgaaaa cgggcactcg ttttgaatgc ttacgaaacg
     1441 gatatacgtt gactggggat tttcttcaat cgcccagcga attgccatgc gcgattcttt
     1501 ttcgtttgac ggctgaatca aagtaagccc cggaatcgaa ccgagtatgg aaatcccgcg
     1561 tacggactga tgggagtgcc cgggcattcc cggaagtatt cctgcgagag acccaacata
     1621 gacaattttt gttttttctg tcgcattatt atagaaatgc tcgtttgcgc gcgttgtcaa
     1681 aaaacattcg aacgaatgaa caatcggaat taaaccttga atcgcaagac cgcccgccat
     1741 tgataccata tcctgctccg caataccgca ttcgatgtaa cgctcgggaa tttctttttt
     1801 aaactggata aggcctgtgt caagcacaag atccgcgtcc attgcgacga cttttttatt
     1861 ctcttttgcg atttttacga gctcgtccgc gtatgctgga atcagacgct ccaaattctg
     1921 gccaattgaa gacgacggca tttccgttga ctcgaactcg agttcaagct cgccctcccc
     1981 ggcattccgc agtttttcgt ttatccgatc cgaaatttct ttgaccgcgg cctcgaattg
     2041 ctcatatgaa ggcgcgccgg aatggaattt atagagccca tcatccgcaa gccgcgtcat
     2101 gaatgagacc ccggcgcctt tcaaagtgtc cgcaataatt acctgcggct tttctttgat
     2161 gctttttgcc cattcaatcg cctttttgat ctcccgcata tcatttccat ttattctcat
     2221 gacttcccat ccaaatgctt taaatttatt ttcgatttcg ccgaggtccg acgtatcttt
     2281 cacgtgagta tctgattgaa ttttattgtg atccacgata actgtgatct ccgagaactt
     2341 cccgtttacg gttggctgga gtgattccca aatctggccc tcttggagtt ctccgtcgcc
     2401 cgtaaggaca tagactcttc cggtctttcc ttgaaggcgt tttcctttgg ccatgccccg
     2461 cgcttttgaa attcccatgc cgagagaacc ggtatttgtc atcaagtaag gcgtctgcct
     2521 gtccgggtgc ccgggaagcc cgccaagccg acgaagcttt tgtatcattt caaacggcag
     2581 tttcccgagg
//
LOCUS       DATDSU010000152         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_984760, whole genome shotgun sequence.
ACCESSION   DATDSU010000152 DATDSU010000000
VERSION     DATDSU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984760"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1219)
                     /locus_tag="VI981_05235"
     CDS             complement(152..1219)
                     /locus_tag="VI981_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HEY4479727.1"
                     /translation="MDVRSIILKKLNERGEVTTAEVTKEAGFSRVYVNKFLNELKDEG
                     KIVLIGRTNRAKYVLANKDIIAKAKHEILGIHRFLQNIDLSEDVVLDEIKRDTGVFID
                     LSQNVNQIVSYAFTEILNNAIEHSKSSEIEVVLRRDESMIRFDIIDRGIGIYNNLMQT
                     RGLKGELEAIQDLLKGKQTTAPEAHTGEGIFFTSKVSDTMTIQSSNKKLIFNNIIEDI
                     FIRDVKNFKGTKVTFSIASDSRKVLEDIFKKYTGDSYEFSKTSVAVRLYKMGTLYISR
                     SQAKRIMTGLEKFRTISLDFKHIKMVGQAFADEIFRIWKSHHPDIEIRTANTNENIDF
                     MIKRAMPKPEEHIGDRPQLFK"
     gene            complement(1566..2123)
                     /gene="rpsI"
                     /locus_tag="VI981_05240"
     CDS             complement(1566..2123)
                     /gene="rpsI"
                     /locus_tag="VI981_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY4479728.1"
                     /translation="MAVKKKEEKIEKPKTPRVKKAVKPTPEVVEHSVVAHVIEVVEKE
                     IPAEFSFDIPKTDRYFEAVGRRKTAVARVRLYTKGDMTTLVNERAYEEYFPTMELQRI
                     VEDPLKKMKSEGRFRISIRVKGGGVHAQAEAARLGISRALTVFNPEFRKRLKRAGFLT
                     RDARAVERKKFGLKKARRAPQWQKR"
     gene            complement(2128..2484)
                     /gene="rplM"
                     /locus_tag="VI981_05245"
     CDS             complement(2128..2484)
                     /gene="rplM"
                     /locus_tag="VI981_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY4479729.1"
                     /translation="MNEVQIDASGRILGRLATVVAKLLMGKGDAAFDYSKPGNMRVVV
                     SHTDKLRVTGKKPLQKLYRRHSGFHGGLKETRYQDLFAKDSRRVLQAAVSGMLPKNKL
                     RVVRLKQLVMYKDGVK"
     gene            complement(2580..>2796)
                     /locus_tag="VI981_05250"
     CDS             complement(2580..>2796)
                     /locus_tag="VI981_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018713499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HEY4479730.1"
                     /translation="ITKAKCAMNAEAPRALALRRQIISVTSPKVAKMLIEKVAPKYKD
                     RNGGYIRIVKTGVRRSDGAQLALIEFI"
BASE COUNT          730 a          702 c          547 g          817 t
ORIGIN      
        1 ctggagagtc tcaagactcg aagaacgcct ccacggtata ccaaaacgaa caaaatagca
       61 agagccagcg aactaccaaa aacctcggca gtgccgaggt ttttgagtgc ccccatccaa
      121 acgcccgcct agcgcttctg cggttttaaa actacttaaa taactgcggc cgatcaccaa
      181 tatgctcctc tggtttaggc attgcccttt taatcataaa gtctatattc tcgttggtgt
      241 tagctgtacg aatttcaata tcaggatgat gactcttcca gattctgaaa atttcatccg
      301 cgaaagcctg accaaccatt tttatatgtt tgaagtctaa agagatagtt ctgaactttt
      361 ccaaccctgt cataatacgt ttcgcttgcg agcgtgaaat atataaagtc cccattttgt
      421 agagtctaac tgcaacgctc gttttactga actcataaga gtctccggta tacttcttga
      481 atatatcctc aaggactttt cgcgagtcgg acgcaataga aaaagttact ttagttccct
      541 taaagttctt aacgtcccta ataaaaatat cttcaataat gttgttaaag ataagttttt
      601 tattcgagct ctggatagtc attgtgtcag aaacttttga agtaaagaaa attccttctc
      661 cggtgtgagc ctccggagca gtcgtttgtt ttcctttcaa aagatcctgg atcgcttcaa
      721 gttcgccttt caagcctcgt gtttgcatca gattattgta aattccaata cctcgatcta
      781 tgatgtcaaa acgaatcata gattcgtcgc gtcgcaaaac aacctcgatt tcgctagatt
      841 tagaatgctc gatagcatta tttagaattt ctgtaaaagc gtaactgacg atttggttaa
      901 cattctggct taggtcaata aatactcccg tatctcgctt aatttcgtcc aaaactacgt
      961 cttccgaaag gtctatattt tgaagaaatc tatgaatgcc caagatctca tgcttcgcct
     1021 tggctataat gtctttatta gctaaaacgt acttcgcacg attcgtcctt cctataagaa
     1081 caatcttgcc ttcgtccttt agttcgttaa ggaatttatt tacgtacaca cgagaaaaac
     1141 cggcctcctt tgtaacttca gccgtagtta cctctccacg ttcattcagc ttttttaaaa
     1201 taatagatct gacgtccata ttgtttacaa gtttagtata ttctaaaaaa actaacttgt
     1261 caacaagtcg ttaaccatac gaccgtagag gaagcagaaa tatggactta cccaccccaa
     1321 aactggctcg atgccggttt tggggtacct ttgttccata gcgaaggacc tcttgcccga
     1381 aacgctcgac cgacgagcaa ccatggtgtc ccgatcgcaa tcgggacgag cgaggaggtt
     1441 ggcgagtctg atttccacgg tagggaaatc agacgttttc ggaaagaggt tcaagctatc
     1501 gtttttgcca ccctaatcct tcgccaaagc tacagacggg tgcaaaggca aaaacgataa
     1561 cgcagttatc gtttttgcca ttgaggagcg cgtcttgctt tcttcagtcc aaacttcttg
     1621 cgctcaacgg cgcgggcgtc gcgcgtcaga aatccagcgc gctttaatct ttttctaaat
     1681 tcaggattaa aaaccgtcag agcccgcgag ataccaagac gcgcggcttc ggcttgggca
     1741 tgcactcccc cgcccttgac tctgattgaa atgcggaatc tgccttccga cttcatcttt
     1801 ttcaaagggt cttccacaat tcgctgcaat tccatcgtgg gaaagtactc ctcataggcg
     1861 cgctcattca ccaaagtcgt catatcgcct ttagtataaa gccgcacccg agcaaccgcg
     1921 gtttttctcc tacccactgc ttcaaagtag cgatcggtct ttggaatatc aaaagaaaat
     1981 tcggcaggaa tttccttctc tacaacctca ataacgtgag ccacaaccga atgctccaca
     2041 acttcagggg tgggttttac cgcctttttc actctcggcg tctttggctt ttcgattttt
     2101 tcctcttttt tctttaccgc cataaactta ttttacccca tctttataca tcacaagctg
     2161 ttttagacgc acaacacgca atttattctt ggggagcatg cccgacactg cagcttgtaa
     2221 aacccggcgc gaatctttag cgaaaagatc ctgatagcgt gtttctttca accctccatg
     2281 gaatcccgaa tgacgccgat agagtttctg caggggcttt ttgcccgtga cgcggagctt
     2341 gtccgtatgc gaaacgacaa cccgcatatt cccgggtttt gaataatcaa aggccgcgtc
     2401 gcctttgccc atcaacaact ttgcgactac agtcgcaagc cgccccagta ttcttccaga
     2461 tgcatctatt tgaacttcgt tcatgtattt agcgacaagc gacaaggcac aagtgacaag
     2521 gtaaatgcac tgaaaaatag ttcatgaact tgttgcttgt ctcttgtctc atgtctctat
     2581 tagatgaatt caatcagggc aagctgtgcc ccatccgacc ttcgcacacc tgtttttacg
     2641 attcttatgt agccgccgtt ccgatcctta tatttcggcg caactttctc aatcagcatt
     2701 ttagctacct tgggagatgt taccgagata atctgccgcc ggagagccag cgcgcgcggc
     2761 gcctccgcat tcatagcaca ttttgccttg gtaatc
//
LOCUS       DATDSU010000153         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2482357, whole genome shotgun sequence.
ACCESSION   DATDSU010000153 DATDSU010000000
VERSION     DATDSU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482357"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VI981_05255"
     CDS             complement(<1..887)
                     /locus_tag="VI981_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY4479731.1"
                     /translation="MHKLNLQRKTSKFYHFGLSFFILIFAFYIFLLPSAADAGFLINR
                     PLYIGLTDGLVGYWSFNGADIAADTAYDRSGNANNGTLTNGPVRTEGKIGQALSFDGT
                     DDYVNLGDMTPTEGQSTLTWAFWTKANYSGNDSCFLCKVELTNQTQNSWGITNTPSSW
                     FGTTRDVRVYIPTSLSDTSTHCNTPNNALTSGAWNHVVVVFDGTQTGNTNRIKFYING
                     TQKAFSAGDCQGTIPASALSSTANAMIGASSDPNNYYNGLIDDVRIYNRVLSPDEIKR
                     LYNIGSTFKINKASNTGSL"
     gene            complement(891..1262)
                     /locus_tag="VI981_05260"
     CDS             complement(891..1262)
                     /locus_tag="VI981_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEY4479732.1"
                     /translation="MENDSVKFKNEFKKRLYSWVLRLIKFIDKLPKDSTCSVMGKQLL
                     RSGTSVLANYIEANSASSKKDFINFFTHALKSANESKVWLTLLRDTEKGDKTELSWLL
                     NELVEIANVLASSIITMKGKR"
     gene            complement(1285..1818)
                     /locus_tag="VI981_05265"
     CDS             complement(1285..1818)
                     /locus_tag="VI981_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479733.1"
                     /translation="MKTSLIIVIIVVLGVVAGAFWYFYRPNVNEGQIWATAETQETLP
                     GWEGYAHPSLPFAFAHSKELVSNTFQDGESEIVLLQGPDPNQEIQIVARAFDEPGPLT
                     VERIQRDVPEMVIDEPQNVLIGSAQIPALLFWSTDNSAGRTREVWFVQGGYLYQVTTK
                     AENDELLAGIMETWRFE"
     gene            complement(1815..2117)
                     /locus_tag="VI981_05270"
     CDS             complement(1815..2117)
                     /locus_tag="VI981_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4479734.1"
                     /translation="MKKKITKKDFINFSEVKRQMFARPGFKVAYDALAPKYALISAML
                     DVRSRRGMTQTEIARRAGTTQSAIARFESGKSNPTLDFAARLSKAVGAKLEVRLKE"
     gene            complement(2245..2451)
                     /locus_tag="VI981_05275"
     CDS             complement(2245..2451)
                     /locus_tag="VI981_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY4479735.1"
                     /translation="MNVIFHPTVLEFLHKLDAQVASNVYRVIEVLRQRGNTLSMPLSK
                     PVGNGLHELRTHGKPAFRVLYGFC"
BASE COUNT          645 a          572 c          588 g          710 t
ORIGIN      
        1 tgtaacgaac cggtatttga tgctttattg attttgaaag ttgaaccgat attataaagc
       61 cgtttgattt cgtcggggct taacacgcgg ttatagatgc ggacgtcgtc aatgaggccg
      121 ttataataat tgtttgggtc cgatgatgct ccaatcatcg cattagctgt cgagcttaag
      181 gcggaagcgg gaatagttcc ctggcaatca ccagcggaaa atgctttttg agtgccgttg
      241 atatagaact taatcctatt ggtattaccc gtctgtgtgc cgtcaaaaac tacaacgaca
      301 tggttccatg ccccgctcgt gagggcatta ttgggagtat tacaatgcgt acttgtatca
      361 gataacgagg taggtatata tactcgcaca tcccgtgtcg tgccaaacca acttgacggc
      421 gtattggtaa taccccacga attttgagtt tggttggtaa gttcaacttt acaaaggaaa
      481 catgaatcgt tgccactgta gttagctttc gtccaaaaag cccaagtcag tgtactttga
      541 ccttcggtag gtgtcatatc accgagattc acataatcat ccgttccgtc aaaggagagg
      601 gcctgcccga ttttgccttc ggtgcggaca gggccgttgg tgagagtgcc attgttagca
      661 ttacctgagc ggtcgtaggc ggtgtctgcg gctatatccg cgccattgaa actccaataa
      721 ccgactaagc cgtcggtgag gccgatgtag agagggcgat tgatgagaaa gccggcatct
      781 gctgccgaag gcagcagaaa aatgtaaaat gcaaatatca aaatgaaaaa tgacaatcca
      841 aaatgataaa attttgacgt tttccgttgt aagtttaatt tgtgcatttt ttatcttttg
      901 cctttcatgg taattatgct agaagcaagg acatttgcta tctcgacaag ctcgtttaaa
      961 agccacgata attcggtttt atctcccttt tctgtgtctc taagaagcgt caaccaaacc
     1021 ttagactcat tcgcggattt cagcgcatga gtaaagaagt taataaaatc tttcttggaa
     1081 ctggctgaat tcgcttctat atagtttgcc agcacacttg ttccacttcg caataattgt
     1141 tttcccatta ctgaacacgt agaatccttt ggaagtttat cgatgaactt tatcaacctc
     1201 aacacccacg aataaagtct tttcttaaat tcatttttaa attttacact gtcattttcc
     1261 attttgattt tttaacttta cattttactc aaatctccac gtctccatta tacccgcgag
     1321 gagctcgtca ttctcggctt tggtggtcac ctgatacaag taccctcctt ggacaaacca
     1381 cacttccctt gtcctgcccg cagagttatc tgtcgaccaa aagaggagcg cgggaatttg
     1441 ggcagagccg atgagcacgt tctggggctc atctatcacc atctcgggca cgtctctctg
     1501 tatccgctca accgtcaagg gcccgggctc atcaaacgcg cgagcgacga tttgaatctc
     1561 ctgattcggg tcgggtcctt ggagtaatac gatttcgctc tcgccgtctt gaaaagtgtt
     1621 cgacacgagc tccttcgagt gggcaaaggc gaagggtaag gacggatgcg cgtagccctc
     1681 ccagccgggc agtgtttctt gagtttctgc ggtagcccag atctgacctt cgttaacatt
     1741 gggccgatag aaataccaaa acgctccggc gacgacgccg agaacaacga tgattacaat
     1801 aattagagaa gttttcattc tttgagacga acttcgagct tcgcacccac tgctttcgag
     1861 agtcgcgctg caaaatcaag cgtgggattc gactttccag actcaaaccg cgcgatagcg
     1921 gactgcgtcg tccccgcacg tcgagcaatc tcagtctgag tcattcccct cctactgcgt
     1981 acatcgagca tagcactgat gagggcgtac ttaggcgcaa gcgcgtcata cgccacctta
     2041 aagcctggac gcgcgaacat ttgtcgcttc acctcgctga aattgatgaa gtcttttttt
     2101 gtgatttttt tcttcatggt agagagtata gcatatacgc tatgtaccgc aatagagacg
     2161 gagacgtttg agggcggtat tgatatctcg cgaatcaaga ttgggtttct gtttctttat
     2221 ggcaactaga agcactgctt ccccttagca aaatccgtag agtactcgga atgcaggttt
     2281 cccatgggtg cgaagctcat gcaatccatt tccaactggt tttgagagtg gcattgaaag
     2341 agtgttacca cgctgtctta ggacttcaat aacacgatac acattggaag ctacttgtgc
     2401 atcgagcttg tgaagaaact caagaacagt tggatgaaaa atgacattca tacgggattc
     2461 atggtgttgg tgtaggttca ggtgttagct ccggagaaag tattagggcg aggac
//
LOCUS       DATDSU010000154        17546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_989534, whole genome shotgun sequence.
ACCESSION   DATDSU010000154 DATDSU010000000
VERSION     DATDSU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17546
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989534"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /gene="rpsR"
                     /locus_tag="VI981_05280"
     CDS             complement(<1..199)
                     /gene="rpsR"
                     /locus_tag="VI981_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003721669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY4479736.1"
                     /translation="MTKQCYFTQNNIRHIDYKDVELLKKFLNPHARMISRKKTGVTSK
                     NQRKLAIAVKRARFMALLPFVA"
     gene            complement(322..786)
                     /gene="ssb"
                     /locus_tag="VI981_05285"
     CDS             complement(322..786)
                     /gene="ssb"
                     /locus_tag="VI981_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY4479737.1"
                     /translation="MYINKALIYGNLTRDPEMRSLPSGIHVTSFSVATNRVYKDKEGN
                     KKEQADFHNVVVFGRQAETVSQYLKKGSGVFVEGRMQTRSWEADGQKKYRTEIVADRV
                     QFGPRSGASTGSPQASGGQAAKGADATEQKAPPPTDEIAYPDEAINADDIPF"
     gene            complement(898..1377)
                     /locus_tag="VI981_05290"
     CDS             complement(898..1377)
                     /locus_tag="VI981_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY4479738.1"
                     /translation="MTTVNEIEKDEEQAEIYEIGFHIIPSISEENVSKTFADIKSIVE
                     KEGGSFIAEDVPKSKALAYALTHVFGGSRQKFTSAYFGWVKFELAAPNIKNVKSALGA
                     DERIIRFLIIKTVRESTLSVARPIHRAEERRERKVEAPKGPVNEVELDKQIEELVKA"
     gene            complement(1732..2595)
                     /locus_tag="VI981_05295"
     CDS             complement(1732..2595)
                     /locus_tag="VI981_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY4479739.1"
                     /translation="MNGSHRWCTVTRIKKGFTLIELLVVVAITSLLASIILANLNQLR
                     NKARIAAGNTLDAQFYHTMANEAYVLWDFNESSGDAVDFSGNNYSGSFVGSVARSSDT
                     KSGSGSSILFNGLSGAYVQSPLISPAIPFPNGITISAWVKLSNGTNDHAIALVEGSSG
                     ALISLSVTGSTLKVEEETSNNFYSLPSVDSGNWMHIAYAYKNSGPQKCAMYVNGREVP
                     ITGGTCGFSPGSINRFYAGRSEDQETRVGSRIDDVRIYADTLLAQDIRDLYLARAASY
                     SSLSLKYRPLR"
     gene            complement(2643..3482)
                     /locus_tag="VI981_05300"
     CDS             complement(2643..3482)
                     /locus_tag="VI981_05300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY4479740.1"
                     /translation="MERERIEFIDVHSHVNFAAYADDYDAVLCRAHDAGVAVINVGTQ
                     IDTSLRAVDLANKYADVYAIIGLHPVHCDKSYHDKKELGEEGKGFVSRGEVFDQSTYK
                     KMALDSKVVAIGECGLDYYRAETDSKYKQKENFIAQIALANEIEKPLMLHIRDAYDDA
                     FEIIKSEAKVRGNVHFFAGNKEQAKRFLELGFTLSFTGVITFTRDCDEIVKYVPLSQI
                     LSETDSPYVTPVPFRGKRNEPAYVVEVVNKISEIKSLNLEDTKKQLLKNAAALFNIPF
                     GVD"
     gene            complement(3484..4917)
                     /gene="metG"
                     /locus_tag="VI981_05305"
     CDS             complement(3484..4917)
                     /gene="metG"
                     /locus_tag="VI981_05305"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY4479741.1"
                     /translation="MSENNSLYITTTIPYVNADPHIGFALEIVHADIFARFESLRGKE
                     VFFNTGSDEHGVKIWKAALASGKSPQAYVDGYAERYRKLKDVLGLLPDINFIRTTSDH
                     HMAAAQEFWKKCAAAGDIYKKYYEIKYCIGCELEKTDSELENGRCPMHPNLEIEFIKE
                     ENYFFRWSKYEKKLIEFYEMNQKFVVPATRFNEIKAFVSRGLKDFSISRLKAKMPWGV
                     PVPDDSDHIMFVWFDAFINYISAIGWPDDMKKFNKWWPVVQVAGKDMVRQQAAMWQAM
                     LMSVGLPPSKQIIIHGFITSGGQKISKSLGNVVHPDALVQEFGTDALRFFLAKEISYL
                     EDSDFTIERFKNSYNADLANGLGNVVGRIMKMANTWLDAPVQTGEPENMDQYFKYLEN
                     FEINKASGFVWSQIKEIDKYIQENAPFTVVKLDKEKGKAMIVQLVKRLYDVATMLEPI
                     LPTTSAKIKMLIKENAVPAVALFPRKD"
     gene            complement(4933..6012)
                     /locus_tag="VI981_05310"
     CDS             complement(4933..6012)
                     /locus_tag="VI981_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4479742.1"
                     /translation="MDKLNRPISITITPGTIVKTILILIFFYFLFAVRDLILVVLTAI
                     VFASSIEPITLWFGKYKIRRLPAVIIIYVGLALLFIGIIYVFLPTLLGDLSTYLANFP
                     QYLDYVHVWLPISESTLADTSERFRQLSTDFSIKDLIGNIQGALANVSEGFVHSVSFI
                     FGGVLSFVIIVVLSFYLAVQESGIEDFLRIITPNRHEAYVIGLWKRSQKKIGLYMQGQ
                     LLLALIVGILVFLGLSILNIKQALLLAFLAAAFEVIPIFGPILSAIPAILIGAAQGGL
                     TTAVIVAGLYIIIQQFENHLIYPLVVKKIVGVSPIVVILALIVGAKLAGFLGVVLSVP
                     VSAAIMEYLHDIQREKVADEHVTIT"
     gene            complement(6444..7715)
                     /locus_tag="VI981_05315"
     CDS             complement(6444..7715)
                     /locus_tag="VI981_05315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4479743.1"
                     /translation="MRFKRKVLKNGMRVITVPMHDNPTVTVLVLVEAGSKYETKNING
                     VSHFLEHMCFKGTAKRLKAIDISRTLDSIGSQYNAFTSYEFTGYYAKSNPRRLSKILD
                     VVSDVYLNSTFPEGEVEKEKGVIIDEINMYEDLPYNDVNSVFMELLYGDQPAGWRITG
                     SKENVRNIRREDIVQYRKNHYVARATTVIVSGHFDEKKIYAEISHRFKNIEEGEKSEK
                     NKIIEQQTSPKILIKKKKTDQTHIVLGVRTYDTYNKKNAVLKVIGVLLGGGMSSRLFQ
                     KLRDEMGVGYYVHSSFDAFTDHGYLDISTGVDSKKAVLVVGAILKELKRLKTERILPA
                     ELQKAKDYIIGNMYLSLESSDSLAEFYGYQEVLKKKIRKPVDIVREIKKVTTGDIEKV
                     AREIFTDDRLNLAMIGPYDSASEFRKIFTIE"
     gene            complement(7765..8349)
                     /locus_tag="VI981_05320"
     CDS             complement(7765..8349)
                     /locus_tag="VI981_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479744.1"
                     /translation="MKPFAFEMTLGMEKDLSKLRLEAMLTDDPSGGLVETYKPSRGKR
                     KYTCVLSPLQGNAACVHVMYHKATRDFSGLGLAGFVDFEGLITFAALMQKEPEMKAFF
                     ASGDGHVLGFGRPAFAERQILNHDAGLAHSMRDLVSPALYFNKGAAQSIFTVYHSEVL
                     VDDCYSLVTIPAAFCSVQSMRAQLRAFETAPTAA"
     gene            complement(8545..9234)
                     /locus_tag="VI981_05325"
     CDS             complement(8545..9234)
                     /locus_tag="VI981_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HEY4479745.1"
                     /translation="MTKYRFFLIVAFLAFWLWAAIDPLYYDGWLLENYLVFIFVPIIL
                     ITGRYFKLSDVSYTVITIFMILHVIGSHYTYAEVPFGFTLQEWFGADRNMYDRLVHFT
                     FGFLLAYPVREVFMRLSHVKGFWAYYFPLDLTLAMSALYEIIEWLAARKVDPAAGIAF
                     LGSQGDIWDAQKDMLLAGCGALIAMLVIFIINWALNKDHWKEFRESFRIPKNDRPLGE
                     VRLREMLKTKK"
     gene            complement(9221..9403)
                     /locus_tag="VI981_05330"
     CDS             complement(9221..9403)
                     /locus_tag="VI981_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479746.1"
                     /translation="MILPINHTSSHQCVRRWQAGILIQAYFEVVIYNITKSKDIPKLY
                     VGSELTSGYNFPHDKI"
     gene            complement(9639..11042)
                     /locus_tag="VI981_05335"
     CDS             complement(9639..11042)
                     /locus_tag="VI981_05335"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY4479747.1"
                     /translation="MAKEEKNTLLDSIVALAKRRGFIYQGSEIYGGLAGTWDYGPLGL
                     ALKNNIKNLWWKRFVDDREDMYGVDAAILMNQNVWKASGHARGFADMLVECSKCKRRF
                     RADHIRSDLKDPEKVGPLSCPECGGSLGEPRQFYMMLETHIGAVQDASSLSYLRPETA
                     QGMFVNFKNVVDSFHPRLPFGLAQIGKAFRNEIAPRDFTFRAREFEQMEIEYFVEPTM
                     WQDSFEHFRKEMWNFVKDVGIDETHVHELEIPDGDRAHYSKRTIDFEFDFPFGRKELY
                     GLAYRTDYDLKAHTEASKVDLSFFDDERKIRFIPHVIEPSFGVDRTVLAVLSDAYRED
                     TMGLKRPLDGRLPDHPQDADDLGEKRVYLALSPRVAPYVAAVFPLLKNKPALVEKARE
                     VYTVLKKELVRVDFDDNGNIGKRYRRQDEIGTPFCITLDFDSLEKEDVTVRGRDTGKQ
                     ERVKVSELADYLKRKTS"
     gene            complement(11046..12950)
                     /locus_tag="VI981_05340"
     CDS             complement(11046..12950)
                     /locus_tag="VI981_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479748.1"
                     /translation="MQEDEHGRIENLKKTLYSRVDRTGQRRKKFEFQRDDITVDEAWR
                     EENPPGNIDMTRKNAPFVKKLFLFSMLFFFLCLFLGFFIFFRGSNIVSSSNIEIAVAG
                     APSVAGGEELVLEIAVTNSNNTALEFADLKVTFPDGSREPGNISEELKRYHESLGEIR
                     SGGTVRRKVRVVLFGEEDSKKEISTVLEYRLKGSNAIFEKEKKLEIAISSAPVTLAVS
                     GLKEVNSNQTVELTVDVTSNSSELLKGMLIKADYAFGFKYETAFPKPAYDNNVWNIGD
                     LAPGTKKTIRIQGKIEGQDGEERNFHFEAGIKGKKNEKELEPVFLSTVHTIVIKKPFV
                     SAQVLLNGSADPIYAAVSGREIKVDVLWENNLTTAVQNAEISVKLPATFIDQSSVKVQ
                     EGHYRSADNTIVWDQTTIRNLERIDPGSSGRLSFYFKTKTIPKDSVEPYRNAEVPLEV
                     MFSGKRPEDANVPEKIKITERKIRLITEATLSAQILYNGGSFQNRGPLPPKANEETTY
                     TVVWSISNSINTVRGAKVAATLPSYVKWLATYSPQNEQVAYNPVNGSVVWTVGDIQAG
                     AGYSGRRREIAFQVVLTPSVSQIGTTPTVVAESVLSGIDSFANVQVQSQRGSLSTKLS
                     NEPSSGTVTQ"
     gene            complement(12981..13574)
                     /gene="recO"
                     /locus_tag="VI981_05345"
     CDS             complement(12981..13574)
                     /gene="recO"
                     /locus_tag="VI981_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY4479749.1"
                     /translation="MAHHKYTTTGFIISSRNSGESNKIFSIITRDFGMIQAGARSVRE
                     VRSKLRLGLKLFALTECSLIHGKNGWKIAGAREIYNIHEACLNDSDKISIVTHIFSLL
                     GRLLTGEEKNESLFFLLDRSFKFLHGANLSTSELKNFECILVLALLAQLGYVGDNPEL
                     ALFIPQPEWSRELLLEMEKIRKIALLHINKSLESSQL"
     gene            complement(13750..17496)
                     /locus_tag="VI981_05350"
     CDS             complement(13750..17496)
                     /locus_tag="VI981_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY4479750.1"
                     /translation="MSDDKEEKVEPKRDRNPVGQGESAIAHEAKQTKSLVAQREEDIL
                     EFWSKNKIFEKSLEQTKNGKPYVFYDGPPFATGTPHYGHILTSALKDAVPRYQTMRGR
                     FVERRWGWDCHGLPIENIVEKDLGISGKKQIEEIGVKKFNEYAKSKVFEYVGIWKKTV
                     ERIGRWVDFDGSYKTMDNSYIESVWWAIKEFYKKGLVYEGTKVLPYCPRCETPIANSE
                     IAMDGSYKDITDISVYVRFELLDDGPSTGSVSSLHASTGQAKTYVLAWTTTPWTLPGN
                     FALAINPDIKYVQVKIEDDPSVYIMAEARVSAVLKDKKYTSLPAFSGKELIGKKYKPL
                     FPYFVDKGSTFLKNQGRTFENAWKIYSADFVTTESGTGVVHVSPGYGEDDMNLARKEN
                     IPFIHHVTHEGKFTNDVTDFAGMSVKPKTAESQGHQVTDIEIIKKLAHEGKLFAKEKI
                     VHSYPHCFRCETPLYYYAIPAWFIKIQSVKNEMLTLNEKINWIPEHLKDGRFKKGLEA
                     APDWNISRNRFWASPLPIWKCDKCARTEFVGSVSEIAEKTKSSNHYTVVRHGEAESNV
                     KGIVSSEPLKYGLTDHGKEQVKEAALSLKKKGKKFDYIIYSPFKRTHETAEILREILC
                     ADKASLISDDRLHEISLGEFNDKPIGVYRASYTSHETYEKHFFKGPPGGESQSDAKRR
                     VGKALQDIDSKYSGKNILLVTHDTPAWLMFSAARGLSIKDTLALHDGSFFLKNAEARA
                     LDFLKLPMNDNFELDLHRPYIDEIRWLCKECEAGTMWRISEVVDCWFESGSMPFAQMH
                     YPFEHREKFHKNFPAQFISEYIAQTRTWFYYTHAVSSVLFKKIAFENVVAHGTVLAAD
                     GSKMSKSKQNFPDPWIVFDRYGVDAVRFYLLSSPIMRSEDLNFSEKEVDGIYKKNILR
                     LRNVLEFYKMYAEPAEVSKAKGQTRKVSHILDRWIVARLEDLHTTVTENLDRYLIDQA
                     LRPFEIFIDDLSTWYLRRSRERLKGHGVDREAAIGTLRFVLLELSKLLAPFTPFIAED
                     VYQTLKGAKESVHLETWPAIGNKFSIFGFDGLTILKDLKDNFQFFRPRRTRLGRAKIL
                     GEMAKVRHIVSLGLEVRSKAGIKVRQPLASLKIRNPNDEIRSEKDLLDLIKDEVNVKE
                     IIFDKNIANEVELDTTITAELKEEGIVREFIRSVQEHRKKCGLVVGDRVTLVVQAEEK
                     GMALLKKYEKNIVKSAQLKDIIPEQTVGESVVIEGLSFVFSIRS"
BASE COUNT         4698 a         4088 c         3612 g         5148 t
ORIGIN      
        1 gagccacaaa cggcaagagt gccataaagc gagcgcgttt tactgcaatc gcgagttttc
       61 gctggttctt tgaagtcacg ccagtttttt tgcgcgaaat catgcgagca tgaggattta
      121 gaaacttctt caaaagttcc acgtccttgt aatcaatgtg cctgatatta ttttgtgtga
      181 agtaacactg cttggtcata agaatttaaa ttcgaaattc gaatatcgaa attcgaaaca
      241 ataactaatt tgtaaatttt caatgtttta aacattcgta ttttgaattt atttaggatt
      301 tcggatttag tgcttcgtgc tttagaatgg tatgtcatct gcattaattg cctcatctgg
      361 atacgctatt tcgtctgttg gtggtggcgc cttttgttct gttgcatcag caccctttgc
      421 cgcctggcct ccgcttgctt gtggcgagcc cgtcgaagcg ccactacgcg gaccgaactg
      481 cactctgtca gctacgattt ctgtacgata ttttttctga ccgtccgctt cccagcttcg
      541 cgtctgcatt cggccttcaa caaaaacccc cgatcccttt ttcaaatact gcgacaccgt
      601 ctctgcctgt cttccaaata cgacaacgtt gtgaaaatca gcttgctcct ttttgttgcc
      661 ttctttgtcc ttgtataccc tattcgttgc gacagaaaaa gacgtaacgt gaattcctga
      721 cggtaaagaa cgcatctccg gatcacgagt taaattccca tatatgagag ctttgtttat
      781 gtacatgtga atatactaat acacgaatgt tacaaataat actaatgaat actcatctga
      841 tcaaggcgtt ttgaattcgt atgcattcgt atattagtgc ccattagtat attggtatta
      901 tgctttaacc agctcctcaa tttgcttgtc gagttcaact tcgttcaccg gaccttttgg
      961 tgcctcgact tttcgttcgc gtcgctcctc ggcgcggtga atcggccggg caacgcttag
     1021 tgtgctttct ctgactgtct tgatgatgag gaaacggatg atcctctcat cggctccgag
     1081 cgcagatttt acgttcttga tgttgggcgc agcgagttca aatttaaccc atccaaagta
     1141 cgcggaggta aatttttgac gactcccccc aaaaacatga gtgagcgcgt atgcaagcgc
     1201 tttgcttttc ggaacatctt ctgcaataaa tgagccgcct tctttttcca caattgattt
     1261 aatgtcggca aaagttttgc tgacgttttc ttcagaaatg gacggtatga tatgaaaacc
     1321 gatctcgtag atttcggcct gttcttcgtc tttttctatt tcgttcactg ttgtcatgtc
     1381 gtctaatggg cacggtgcaa aaagcacagt gggtacagac tatcacagcc tttttttcta
     1441 tgcaaattaa ggcttattga gactgtttta ttgaaaatgt ctccgtctga cacttaaaaa
     1501 aacgcggttt tttattttgg ctagcgagga gcgggtgaac gtgtcttcac gttcacatcg
     1561 tccgagcgaa gacaaaatat atgattggag tcacatataa ttgcgtcatt cgttcgaaat
     1621 gaatatgaag acatattcat ttctccctca ctagcaatta taggtctagt accgcgcggt
     1681 cttgccgcgt gctggacgag cgaagcgagt tcctagatta ccctgcggtt tttaccgcag
     1741 gggcctatat ttgagcgaga gactgctata actcgcggcc ctcgcaaggt agaggtctcg
     1801 tatatcttga gcgagaagcg tgtcagcata aattcgcacg tcatctattc tggaaccaac
     1861 tctcgtttcc tggtcttctg accgaccagc ataaaaccta tttatcgaac cgggactaaa
     1921 gccacacgta cctcccgtaa tcggcacctc cctaccgttt acatacatgg cgcatttttg
     1981 gggacccgaa ttcttatatg cataagcaat gtgcatccag tttccagaat caactgacgg
     2041 aagcgaataa aaattatttg aagtttcttc ttcaaccttt agcgtcgatc cagttaccga
     2101 gagagagata agtgcaccgc tcgatccctc tacgagagca atagcgtgat cattggtgcc
     2161 attacttaat ttaacccagg cagagattgt tatcccgtta ggaaaaggta tcgcgggaga
     2221 tatgagcggt gactgcacat aagcgccgct taaaccatta aataggattg atgaaccgga
     2281 gccgcttttt gtgtcagaac ttctcgctac acttccaaca aatgacccgc tatagttatt
     2341 ccctgaaaag tcaacggcat cgccgcttga ttcattgaaa tcccatagta catacgcttc
     2401 gtttgccatt gtatgataaa actgtgcatc aagcgtattg cccgccgcaa tacgagcttt
     2461 atttctcaat tggttgaggt tcgccaggat gatcgaggcc agaaggctgg tgattgcaac
     2521 aacaacaaga agctcaatta aggtaaaacc tttctttatt cttgtgacag tacaccaacg
     2581 atgagaacca ttcatatgca aattattata acattaccaa aagggggaca tttaatctcc
     2641 ggtcaatcga cgccaaatgg aatgttgaac aatgccgcag catttttgag caattgcttt
     2701 tttgtatctt caaggttcaa actttttatt tccgatattt tgttgacaac ctcaacgacg
     2761 taggcaggct catttctttt gcctcgaaat ggaactggag taacataggg agaatctgtt
     2821 tcagacagta tctgcgaaag aggaacgtac tttactattt cgtcacaatc acgtgtaaaa
     2881 gtaatcactc ccgtaaagga gagcgtaaaa cccaactcta aaaaacgttt tgcctgttct
     2941 ttattgccgg caaagaaatg cacattacct cgtactttgg cttcagactt aatgatttca
     3001 aaagcgtcat cgtacgcgtc cctaatatgg agcatgagag gcttctcgat ctcattggcg
     3061 agcgcgattt gcgctataaa attctctttt tgtttatatt ttgaatccgt ttccgctcga
     3121 taataatcaa gaccgcattc gcctattgcc acgacttttg agtcaagtgc cattttttta
     3181 taagtagatt gatcaaagac ttcgcctcgt gatacgaaac cttttccttc ttcgcctaac
     3241 tcttttttat cgtgatatga cttatcgcaa tggactgggt gtagaccaat gattgcgtag
     3301 acatcagcat atttatttgc caagtcaact gcccgcagcg aagtgtctat ttgagtaccg
     3361 acatttatga ctgccacgcc tgcgtcgtgc gcacgacaca agacggcgtc gtagtcatct
     3421 gcatatgcgg caaaattaac gtgcgaatgg acgtcgataa attctattct ttcgcgttcc
     3481 atatcaatcc ttgcggggaa aaagagcaac tgcgggaacc gcattttcct ttataagcat
     3541 ttttattttt gctgacgtcg taggcaggat cggttcgagc atagttgcca catcatataa
     3601 acgcttgacc agttggacaa tcatagcctt tcccttttcc ttgtctaact tcactacggt
     3661 gaatggcgcg ttttcctgaa tgtatttgtc gatttctttt atttgactcc acacgaagcc
     3721 cgacgcttta ttgatttcaa aattttcaag atacttgaaa tattgatcca tattttcagg
     3781 ttctccggtt tgaaccggtg cgtcgagcca ggtatttgcc attttcatga ttcgacctac
     3841 aacgttgccg agtccgtttg cgagatcggc attgtaggaa tttttaaaac gttcaatggt
     3901 aaaatcgcta tcttcaagat aagaaatttc tttggcgaga aaaaaacgta aggcatcagt
     3961 gccgaattcc tgaaccagag catctggatg aacaacattg ccgagactct ttgatatttt
     4021 ctgtcctcca cttgtgataa agccatggat aataatctgc ttcgaaggag gcaatccaac
     4081 agacatgagc atggcctgcc acattgcagc ctgctgacgt accatatctt ttccggcaac
     4141 ctgaacgaca ggccaccatt tattaaactt tttcatatcg tccggccagc cgatcgcaga
     4201 aatatagttt ataaacgcat cgaaccagac gaacataata tgatcgctat catccggtac
     4261 aggcacgccc caaggcattt tagccttcaa tcgcgagatg ctaaagtctt ttagacctcg
     4321 agaaacaaaa gccttgattt cattgaatct ggtcgccgga accacgaatt tttgattcat
     4381 ttcgtaaaat tcaataagtt ttttttcata tttagaccac cgaaaaaaat agttttcttc
     4441 tttgatgaat tcaatttcca gattggggtg catcgggcat ctaccgttct cgagttctga
     4501 atcagttttt tcaagttcgc aaccgatgca gtactttatt tcgtaatact ttttgtaaat
     4561 atcgcccgcg gcggcacact tcttccaaaa ttcctgtgca gccgccatgt gatggtcgct
     4621 cgtcgtccga atgaaattta tgtccgggag gaggccgagc acgtctttga gctttctata
     4681 tctttctgca tatccgtcga cataggcctg cggactttta ccactcgcga gcgccgcttt
     4741 ccatattttt acgccatgct cgtcacttcc cgtgttaaaa aaaacttcct tgccgcgaag
     4801 agactcgaag cgagcaaaaa tatctgcgtg aacaatttca agcgcaaatc cgatgtgcgg
     4861 gtctgcgttt acatatggaa ttgtggttgt aatgtataga gaattattct cgctcattcc
     4921 gttagattcg atttacgtta tagtcacgtg ttcgtccgct actttctcac gctgaatatc
     4981 gtgcaaatat tccatgattg cagcggaaac aggcaccgat agtaccacac ctaaaaatcc
     5041 tgccaatttt gccccgacaa taagagcgag aatgacgacg ataggcgaaa cgccgacaat
     5101 ttttttaacg acaagcggat aaataaggtg gttttcaaat tgctggatga taatgtaaag
     5161 tccggccacg ataacggcag tagtcagtcc tccctgggcc gcgccaatca aaatagccgg
     5221 tatcgccgaa agtatgggac caaaaatagg aatgacctca aacgccgcgg ccaaaaatgc
     5281 caaaagaagg gcttgtttaa tattcagaat tgaaagccct aagaatacca atataccaac
     5341 gataagcgcg agtagaagct gtccttgcat gtagagacca atttttttct gcgaacgttt
     5401 ccacaaacca atcacatatg cctcatgacg gttcggcgtg atgatgcgca gaaaatcttc
     5461 aatcccagat tcctgcactg ccaaataaaa tgaaagcact actataatca cgaacgagag
     5521 cacaccgccg aaaataaaac tgactgaatg cacaaaacct tcggagacat ttgcgagcgc
     5581 tccctgaata ttaccgatca aatcttttat tgaaaagtca gtggataatt gccggaatcg
     5641 ctccgaagta tcggcaagcg tcgattcact gattgggagc cagacatgca cgtagtctag
     5701 gtattgggga aaatttgcca gataggtgga caagtccccc aagagtgtgg gtaggaaaac
     5761 atagataatg cctataaata aaagtgccag gccgacataa atgataatga ctgccggaag
     5821 acgtctgatt ttgtacttac cgaaccatag tgtaatcggc tcaattgacg atgcgaagac
     5881 gattgccgtt agaacaacta atataagatc acggacggca aaaagaaagt agaaaaaaat
     5941 caaaatcaag attgtcttga cgatagtgcc aggtgtgatt gtaatcgata ttgggcggtt
     6001 gagtttatcc atcccgttta gaaacccgcc tgccatcgcc tgacacaccg atgggaggac
     6061 gtgtgattaa ttggtaatag catcttcatt ttattatagc tactgcgtat ttcgctctgg
     6121 ctgtggcggg caggtaggat gcgcccagca tacgtgccga gtaagcgcaa ttggttagta
     6181 atctacacac atatacaagt ctggtcatta ttggtgaagg agcatccgtt acgtctatta
     6241 caaacatcgg ttttctaaaa ttaatcgatt ttgttaaatt aggtcgtaaa tacataacac
     6301 tttaatttct aacgggatcc atcattatta aaataatagc atgaatagta tcgatgcgaa
     6361 tatacaaatt atgcgaatgc tacaaataga attcaagact tattcacttg atttgttcga
     6421 gccgtcggtt aatatcgata ggattattct atcgtaaata tctttctgaa ctcgctggcg
     6481 ctatcatacg ggccgatcat agccaagttc aatctatcgt ctgtgaatat ttctcgagca
     6541 actttttcga tatctcctgt cgtgactttt tttatttcac gaacaatatc gactggtttt
     6601 cgaattttct ttttgagaac ttcctggtag ccgtaaaatt cagcaagaga atcagaagac
     6661 tcgagagaaa ggtacatgtt gccgataata tagtctttgg ccttttggag ttccgcgggc
     6721 aaaatgcgct ccgttttgag tcgtttcagc tccttcaata tagccccgac aacaagcacg
     6781 gcctttttac tatctacgcc ggttgaaata tcgagatatc cgtgatcagt aaacgcatcg
     6841 aatgacgagt gaacataata tcccactccc atttcatctc ggagcttttg gaaaagtctt
     6901 gaactcattc cgccgcccaa taatactccg atgactttga gtacggcatt ctttttgttg
     6961 tatgtatcat atgttcggac tccaagcacg atatgggtct ggtcggtttt tttcttctta
     7021 atgagaatct tgggcgacgt ttgctgttcg atgattttat ttttttcact cttttcccct
     7081 tcttcgatat ttttaaatcg atgtgaaatt tccgcgtata tttttttctc gtcaaaatga
     7141 ccagatacaa ttactgtagt cgccctagcg acatagtgat ttttccggta ttggacgata
     7201 tcctcgcgcc ttatatttct cacattctct ttgctacctg taattctcca tcctgcgggc
     7261 tgatctccgt aaagcagttc cataaagaca ctattcacat cgttgtacgg caaatcctcg
     7321 tacatattta tttcatcaat aatgacgcct ttttctttct ccacttcgcc ttcgggaaag
     7381 gtggagttca aatagacatc agaaacaacg tctagaattt tactaaggcg cctcggatta
     7441 gactttgcat aatagcccgt aaattcataa gaggtaaatg cattgtattg agagccaata
     7501 ctgtcgagcg tgcgtgaaat atcaatagcc ttcagtcgct tggccgttcc cttgaaacac
     7561 atgtgctcaa gaaaatgaga tacgccgttt atgtttttcg tttcgtattt agaacctgcc
     7621 tctacgagga cgagcacagt cacagtcgga ttatcgtgca tgggcactgt tatgaccctc
     7681 attccatttt tgagtacttt tcgcttgaaa cgcatttgac cattatacac aatcgaaaaa
     7741 aatgacaaga aaaaagcggc gcgattatgc cgctgttggt gctgtttcaa aagctcggag
     7801 ttgcgctcgc attgactgaa cgctacaaaa cgccgcggga attgtaacaa gcgagtagca
     7861 gtcgtcgaca agaacctcgc tgtggtacac agtaaatatt gattgcgccg cccctttgtt
     7921 gaagtacagc gccggagaca cgagatcccg catcgaatgc gccagacctg catcatggtt
     7981 caggatttgc ctttcagcga acgcgggtcg gccgaaacca agtacatgac catcgccgct
     8041 cgcaaagaaa gccttcattt cgggctcttt ctgcatgagc gcggcaaaag tgataagccc
     8101 ttcgaaatcg acgaaccccg ccagtcccaa accgctaaaa tcgcgcgtcg ccttgtggta
     8161 catcacgtgt acacacgcgg cattaccctg aagcggcgag agaacgcaag tgtactttcg
     8221 cttgcctcga gatggcttat atgtttcgac gagcccccca gacggatcat ctgttagcat
     8281 cgcttcgaga cgtagttttg aaaggtcttt ttccatccca agggtcatct caaatgcaaa
     8341 tggtttcatg gtgtttctgt aatttggttg taggtttttc tttcatttaa ataccacata
     8401 ttcaaatcaa gtgcaagttt ttcgttcttc tgtcatctgt tatcacaagt acaaaatcct
     8461 cgtcatacaa ctataaattg ctatagcaat ttatagttgt atttgctccc ttaatagttc
     8521 ggatactttt ttcgagcaat gattctactt tttagtctta agcatttctc tcagcctgac
     8581 ctctccgaga ggcctgtcgt tttttggaat tcgaaaactt tctctaaatt ctttccaatg
     8641 atctttgttt agagcccagt taatgatgaa aataacaagc atagctataa gagctccgca
     8701 tccggcgagc agcatatctt tttgagcatc ccaaatatcg ccctgactgc ctagaaaggc
     8761 gattccggcc gcaggatcaa cttttctggc cgcaagccat tcgataattt cataaagtgc
     8821 cgacatagcg agagtgaggt caagcggaaa ataataggcc caaaaacctt ttacgtgaga
     8881 aagacgcatg aatacttctc gtacgggata cgcaagcaga aaaccaaacg tgaagtgtac
     8941 gagcctgtca tacatatttc ggtcggctcc aaaccattct tgcaacgtaa acccgaaagg
     9001 aacctcggca tacgtgtaat gcgaaccgat aacgtgcaga atcatgaaaa tcgtaataac
     9061 cgtatacgaa acatccgaga gcttgaaata ccgtccggtg atgagaatga tcggtacaaa
     9121 aataaatacg agataatttt caagcagcca gccgtcataa tagagaggat caatagcggc
     9181 ccaaagccag aaagcgagaa aagcaactat caaaaagaat ctatattttg tcatgcggaa
     9241 aattataccc cgaagtgagc tcacttccca catagagctt gggaatatct tttgacttag
     9301 taatgttata aattaccact tcaaaatagg cctgtatcaa gatccccgcc tgccatcgcc
     9361 tgacacactg atgggaggac gtgtgattaa ttggtaatat catcttcttg tattaaagct
     9421 actgcgtatt tcgctctggc tgtggcgggc aggtacttcg gggttatagc aggtcaaatg
     9481 aaatatgctt gtgcaaaaac gagatgcatc cctgttaaca ataagatcat agatacataa
     9541 ttcgtcaaca tacattaatc ttacgcacgt gcgtaaaatt aatgtatacc gaaaagatgt
     9601 tataaaggtg ttataaggtt tctggatcga aactattttt aactcgtctt tcgttttaga
     9661 taatccgcaa gttcggaaac ttttactcgc tcctgcttcc ccgtatcgcg accgcgcaca
     9721 gtcacatctt ctttttcaag cgaatcgaag tctaaagtta tacaaaacgg cgtgccgatc
     9781 tcatcctgtc gtcggtagcg tttgccgata ttgccattat cgtcaaaatc aactctaact
     9841 agttcttttt taagcactgt atagacttcg cgcgctttct caacaagcgc cggcttgttt
     9901 ttcaagagcg gaaagacagc ggcaacgtac ggcgccactc gaggcgaaag agcaagatag
     9961 actcttttct cccctaaatc atccgcatcc tgcggatgat cgggcaaacg gccatccagt
    10021 ggccgtttta gccccatcgt atcttcccga tatgcgtccg agagtacagc aagaacagtt
    10081 cgatcaactc caaaagacgg ttcaatgaca tggggaatga aacgtatttt cctttcatcg
    10141 tcaaaaaacg aaagatcaac tttactagct tccgtgtgtg cctttaaatc gtaatccgtt
    10201 cgatacgcaa gaccgtaaag ttctttccta ccgaaaggaa aatcaaattc aaaatctatc
    10261 gttctttttg agtaatgagc tcgatcaccg tccggaattt caagttcgtg gacatgtgtc
    10321 tcatctatac ctacatcttt aacaaaattc cacatctcct ttctgaaatg ctcaaatgaa
    10381 tcctgccaca tggtcggctc gacaaaatat tcaatttcca tctgctcaaa ctcgcgagcg
    10441 cgaaaagtga agtctcgggg cgcgatttca ttcctaaatg ccttccctat ctgcgcgaga
    10501 ccgaacggca atctcggatg aaacgagtca acgacatttt taaaattgac gaacattcct
    10561 tgtgccgttt cgggacgaag gtatgacaat gacgaggcgt cctgtacagc tccaatatgg
    10621 gtttccagca tcatgtaaaa ttgacgcggt tcgccgagag atccgccgca ttcgggacaa
    10681 gataaaggtc cgaccttttc ggggtcttta aggtcggacc ttatgtgatc ggctcgaaat
    10741 cggcgtttgc acttagaaca ctcaacaagc atatcggcaa aaccgcgtgc atgacccgat
    10801 gctttccaaa cattctggtt catcaggatt gcagcatcta cgccatacat gtcctctcga
    10861 tcatcaacaa aacgcttcca ccacagattt ttaatattat tttttagcgc cagtccaagt
    10921 ggtccatagt cccatgtgcc agccagaccg ccgtaaattt cagaaccctg atagataaaa
    10981 ccgcgacgct tcgctagcgc cacaatcgaa tcgagtaaag tatttttctc ttctttggcc
    11041 atacactatt gtgtaactgt gcccgaactg ggctcgttgc ttaattttgt cgaaagtgag
    11101 ccgcgctgtg actgtacctg aacattggca aaactgtcga ttccgcttaa cactgattct
    11161 gcaactacag tgggagtcgt gccgatttgg gatacacttg gcgttagcac tacttggaaa
    11221 gcaatctcgc gacggcggcc agagtatccg gcacctgcct gaatgtcacc cacagtccat
    11281 accacactac catttaccgg attgtaggca acttgttcgt tctgcggact gtaagtggca
    11341 agccatttga cgtaacttgg caatgtcgcc gcaacctttg ccccccgaac cgtgttgatg
    11401 ctgttggaaa ttgaccaaac taccgtatag gtcgtctcct cgtttgcctt cggcggaagt
    11461 ggtcccctat tttggaatga accgccattg tagagaattt gagccgaaag cgtcgcctcg
    11521 gtaatcaatc taattttccg ctctgtaatc tttatttttt ctggaacatt ggcgtcttcg
    11581 gggcgcttac cggaaaacat cacttctaac ggaacttccg catttcggta cggttcgacg
    11641 gagtctttcg gaattgtttt tgttttaaaa taaaacgaga gccgacccga ggaacccgga
    11701 tcaattcgtt cgagatttcg aatggtcgtc tgatcccaga caatagtgtt gtcggcagag
    11761 cggtaatgcc cttcctgcac ttttacagat gattgatcga taaacgtggc cggcaattta
    11821 actgaaattt ccgcattctg cacagccgtc gtgagattgt tctcccataa cacatcgact
    11881 tttatttctc tgccggaaac agcggcataa atcggatctg cggaaccgtt taagagcact
    11941 tgcgcgctca caaacggttt ttttatgact atcgtatgta cggttgagag aaaaaccggc
    12001 tcaagttcct tttcattttt tttgcctttt atacccgcct caaaatgaaa gttccgctct
    12061 tccccgtctt gtccttcgat tttgccctgg atacgaatcg ttttcttggt tccaggcgct
    12121 aaatcaccta tattccaaac gttgttgtca tatgctggtt tcggaaaagc cgtctcatac
    12181 ttaaaaccaa acgcatagtc agcctttata agcatccctt tcaataactc actagaattt
    12241 gaggtgacat cgacggtcag ttctactgtt tggtttgagt ttacttcttt caacccagaa
    12301 acggcgagcg tcaccggagc agatgagata gcgatttcaa gctttttttc tttctcaaaa
    12361 atcgcgtttg aaccctttag tctgtactca agaacggtgc ttatttcttt tttgctgtct
    12421 tcctctccaa agagaacaac gcgaactttc cgtcgcaccg ttcctcctga ccggatctcg
    12481 ccgagcgatt catgatagcg tttcaactct tcgctgatat tgcccggctc tcgagaaccg
    12541 tcgggaaatg taaccttaag gtcggcaaac tcaagcgccg tgttgttact gtttgttacg
    12601 gcaatttcga gtacaagctc ttccccgccg gcaacggatg gcgcgcccgc caccgcaatt
    12661 tcaatattac ttgacgagac gatatttgat cctctgaaga aaataaaaaa acccaagaat
    12721 aaacaaagaa aaaagaagag catggaaaac agaaacagtt tttttacaaa aggtgcgttt
    12781 tttcttgtca tgtctatatt tccgggcggg ttttcctccc gccatgcctc atcgacggta
    12841 atatcatcgc gctgaaattc gaattttttc cttcgctgtc ctgtgcggtc tactcttgaa
    12901 tagagtgttt tcttcaggtt ttcaattcgc ccatgctcat cttcttgcat gaagaaaatt
    12961 atagcatgtt catacgtagc ctatagttgt gacgattcga gagatttatt gatatgaagc
    13021 aaggcaattt ttcttatttt ttccatctca agcaagagtt ctcgtgacca ttcaggctgc
    13081 ggaataaaga gcgcaagttc agggttgtcg ccaacatacc caagctgtgc gagcagcgcc
    13141 aggacaagaa tacattcaaa atttttaagc tccgacgtag agagattcgc tccgtgtaag
    13201 aatttgaaac tgcggtcaag caaaaagaag agcgattcgt ttttttcttc tcccgtaagc
    13261 agccggccaa gcagtgagaa tatgtgcgtg acaatagata ttttgtcgga atcattgaga
    13321 cacgcttcat gaatgttgta tatttccctc gcaccagcaa tcttccagcc attttttcca
    13381 tggataagac tgcactctgt cagagcaaag agttttaatc ccaaacgcag ttttgaccgt
    13441 acttcccgaa cagatctcgc cccagcctga atcatgccga aatcgcgcgt tatgattgaa
    13501 aaaattttat tcgactctcc gctatttctt gaggaaatta taaaaccggt tgttgtgtat
    13561 ttgtgatggg ccatgtacta gcgtatagct tttagggttc agggttcagg gtttagacaa
    13621 agtgatttag cacagaattt agtatatcaa gattcaggtc aacgtgtttt gtaaataaga
    13681 tacaacccag cactaaattt tgcgaaaccg cagagaaaat ttatcctgca tggggcacaa
    13741 aaatatacgt caggaacgaa tggaaaaaac aaatgaaagg ccttctataa caacgctctc
    13801 gcccactgtt tgttcgggta ttatatcttt gagttgggca gatttcacaa tatttttttc
    13861 gtatttctta agcaacgcca tgcccttttc ttctgcctgc acaacgaggg tcacgcggtc
    13921 acctacgaca agtccgcatt tttttctatg ctcttgaacc gagcgaataa attcacgcac
    13981 gattccctcc tcctttagct ctgctgtaat agtcgtgtcc aactccactt cattcgcgat
    14041 atttttatca aagataattt ctttcacatt aacttcatct ttaatcaaat ccagcaagtc
    14101 tttttcgctt cggatttcat catttggatt tcggatttta agcgaagcga gcggttgccg
    14161 tacttttatt ccggcttttg agcgcacctc aagcccgagc gagacaatat ggcgcacttt
    14221 tgccatttct ccaagaattt ttgcccgccc gaggcgggtc cgccttgggc ggaaaaattg
    14281 aaaattgtcc ttcaaatcct tcaggatggt gagcccgtcg aatccaaaaa ttgaaaattt
    14341 atttccaatg gcaggccacg tctcaagatg gacgctctcc tttgcgccct taagtgtctg
    14401 gtagacatct tctgcaataa aaggcgtaaa cggagctaga agtttagaca attccaagag
    14461 cacaaaacgc aaagtgccaa ttgccgcttc tctgtcaact ccatgtcctt tcagacgctc
    14521 cctcgatcgc cgcaaatacc atgtagacaa gtcgtcaata aagatttcaa aaggccgtag
    14581 cgcctgatca atgagatatc gatctagatt ttcggtcact gtggtatgca aatcttccaa
    14641 ccgggcgaca atccagcgat caagtatgtg agacactttt cgcgtctgac ctttagcttt
    14701 tgagacctcg gccggttcgg cgtacatttt gtaaaattcg agtacatttc taagccttaa
    14761 aatattcttt ttatagattc catcaacttc tttttctgaa aaattgagat cctcggagcg
    14821 cataataggg ctcgataaga gatagaatcg aactgcatcg acaccgtatc ggtcaaaaac
    14881 gatccacgga tcagggaaat tttgttttga ttttgacatt ttcgagccgt ctgcggccag
    14941 aactgtaccg tgcgcgacaa cattctcgaa tgctatcttt ttaaacaaca cggacgaaac
    15001 tgcgtgggta tagtaaaacc aagtacgagt ttgagcgata tattctgaga taaattgagc
    15061 cggaaagttt ttatggaatt tttccctatg ctcgaaagga taatgcatct gggcaaacgg
    15121 catcgaacct gattcaaacc agcaatccac cacctcggaa atacgccaca tcgttcctgc
    15181 ttcgcattct ttacatagcc agcgtatctc atcgatatac ggacggtgca gatcaagctc
    15241 gaagttatca ttcataggca atttcaaaaa atctagcgcc cgtgcttccg cgtttttaag
    15301 aaagaatgag ccgtcgtgca gtgctaatgt atccttgatc gaaagtccgc gtgcggcgga
    15361 gaacatcagc caagccggcg tgtcgtgtgt tactaatagt atgtttttgc cgctgtattt
    15421 tgaatcgatg tcttgaagcg ccttgccgac ccgccgcttc gcatcacttt gactttctcc
    15481 ccccggcggg cccttaaaga aatgtttttc gtatgtttcg tgcgacgtgt aagaagcgcg
    15541 atacacccca atcggcttat cgttaaattc acccaaagat atttcatgga gtctgtcgtc
    15601 agaaataaga ctcgccttat cagcacacaa aatctctctc aaaatttcgg ccgtctcatg
    15661 tgttcgtttg aacggtgaat aaataatgta gtcgaatttc ttaccttttt tcttgagaga
    15721 aagagccgcc tcctttacct gctccttgcc atgatcagtc agtccatatt ttaatggttc
    15781 ggagcttaca atccccttta cgttactctc ggcttcacca tggcgaacaa ctgtatagtg
    15841 atttgaactt tttgtttttt cggcaatttc tgaaaccgat ccgacgaact cggtgcgggc
    15901 gcatttgtcg catttccata tgggcaaggg cgacgcccag aaacgattac gggaaatatt
    15961 ccaatcgggt gccgcttcaa gacctttttt aaaacgccca tctttcaagt gctccggaat
    16021 ccaatttatt ttttcgttca gcgtgagcat ctcattcttt acagactgaa ttttaatgaa
    16081 ccatgccgga attgcgtaat aataaagcgg ggtctcacag cggaaacagt gcgggtatga
    16141 gtggactatt ttctccttag cgaagagttt gccttcgtgt gcgagttttt tgataatctc
    16201 gatgtcggta acttgatgac cttgagactc cgcggttttc ggtttgacgc tcatgccggc
    16261 aaaatccgtc acgtcgtttg taaattttcc ttcatgggta acgtgatgga taaatggaat
    16321 attttccttt ctcgcgagat tcatgtcatc ctcaccgtaa cccggggaaa cgtggacaac
    16381 gcccgttcct gattcagttg tgacaaagtc cgcactataa attttccacg cattttcgaa
    16441 ggtccgacct tgattcttca gaaaggtcga acctttatct acaaaataag gaaataatgg
    16501 cttgtatttt tttccgatca attctttacc actaaacgca ggcaatgaag tatatttttt
    16561 gtcttttaga accgcagaga ctcgcgcttc ggccataata tagacgctcg gatcatcttc
    16621 aatctttacc tgaacatatt tgatatcagg gttaatcgcc aatgcaaaat tgcccggaag
    16681 tgtccatggt gttgtagtcc aagctaagac atacgtctta gcttgcccgg tgcttgcgtg
    16741 cagtgagctt accgaacctg tcgaagggcc gtcatctaaa agttcaaacc taacatatac
    16801 tgaaatatcc gtgatatctt tgtaacttcc atccattgca atttcagaat ttgcgatagg
    16861 cgtttcgcac cgcggacagt atggaagcac ttttgtgccc tcatagacga gccccttttt
    16921 atagaattct tttatcgccc accatacaga ctcgatatag gaattgtcca tcgttttgta
    16981 agaaccgtcg aaatctaccc accgaccgat tcgctccacc gtttttttcc atatgcccac
    17041 atactcgaat accttgctct ttgcatactc attgaatttt tttacgccta tttcctctat
    17101 ttgtttcttg cccgaaatcc cgagatcttt ctcgacaatg ttttcgatcg gcaatccgtg
    17161 acaatcccac ccccatcgcc gctcgacgaa gcgccctcgc atggtttgat aacgcggcac
    17221 cgcatccttg agggcgctcg tcaaaatgtg gccgtagtgc ggcgtgccag tcgcgaaagg
    17281 cggaccgtca taaaaaacgt atggtttgcc gtttttcgtt tgttcaagcg atttctcaaa
    17341 aattttattt ttactccaaa attcgagaat atcttcttct ctttgggcga ccagactttt
    17401 ggtttgtttg gcctcatgtg caatagcact ctctccttgg ccgacgggat ttcgatcccg
    17461 tttaggctcc actttttcct ccttatcgtc cgacatacaa aatttatctt agcacctaat
    17521 cttttaaaca aaaaaatggc taaacg
//
LOCUS       DATDSU010000155         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2487507, whole genome shotgun sequence.
ACCESSION   DATDSU010000155 DATDSU010000000
VERSION     DATDSU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2487507"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VI981_05355"
     CDS             complement(<1..460)
                     /locus_tag="VI981_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479751.1"
                     /translation="MKSPRDTGTKLTPYILIIVSCLLIVSLNSRDAEAARIDELRQKI
                     DEKNNTIKQLEEEIAQFTKDVAETGKQAKTLESALKTLDLTRQKLVKDIALTENKIAT
                     AELEIERLSFDITEKAVDIDVLRKALARNMRIRNAEDQNSLLEVLLAYKNL"
     gene            complement(457..1797)
                     /gene="gltX"
                     /locus_tag="VI981_05360"
     CDS             complement(457..1797)
                     /gene="gltX"
                     /locus_tag="VI981_05360"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY4479752.1"
                     /translation="MPLQNISEKVITRFPPSPTGFFHIGSARTALFNYLFAKKNNGVL
                     YLRFEDTDKARSKKEYEDDIRAGLEWLGIECTLPEVARQSERMHIYRAQFTKLLESGA
                     AYEAESTADDKSKKVIRFKNPNIRIVFQDLIRGEVSFDTTELGDFVIAKSLEEPLYHL
                     AVVIDDHEMAVTHVIRGEDGISNTPRQILILEALGFTRPSYAHIPLILAPDRSKLSKR
                     HGAVSVTEYKNMGFLSEALVNYLALLGWNPGGDKEIFNMDELISEFDLSRVHKAGAIF
                     SEEKLRWFNKEHLKKNGNYQISIINEVRKLFPEADDQIVKKIVPIVFERIEVLSDIKK
                     LADNGELLYYFSQPTYKKEDLHWKGEKDTDNTKIYLTETAAKLESLDKTFDADTVRKA
                     LWTYAEEKGKGNVLWPLRFALSGREKSPDPFTLASILGKNETLRRIRRAAELLS"
     gene            1894..>2311
                     /locus_tag="VI981_05365"
     CDS             1894..>2311
                     /locus_tag="VI981_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479753.1"
                     /translation="MKTTALTFAPTSDVTLRQCFAVGARSENTGIGIPLRIFFPLLSQ
                     PEYHVLAVPGLCYSEELFYPWASALAAYGIETAVMAYDPMRSPKEKTLARCANDIASA
                     VAHLGHSPLVILAHSAPALAIRLLTPEQKGKHTWVIA"
BASE COUNT          554 a          557 c          487 g          713 t
ORIGIN      
        1 agagattttt gtatgcaagc aaaacttcaa gcaaggaatt ttgatcctcc gcattgcgga
       61 tcctcatatt tcgcgcaagt gccttccgta acacgtcaat atccacagcc ttctcggtga
      121 tatcgaatga aagccgctca atttccagtt cggctgtggc gatcttattt tcagtgagtg
      181 caatatcttt tacgagcttt tgcctggtaa gatcgagtgt cttcaaggcg ctctccaaag
      241 tcttagcctg tttgccagtt tcagcgacgt cctttgtgaa ttgggctatt tcttcctcaa
      301 gttgcttgat agtgttgttt ttctcatcta ttttttggcg caattcatcg attcgcgccg
      361 cttcggcgtc cctgctattt agagacacga tgagtaagca acttacaatg ataagaatat
      421 acggcgtgag ctttgttcca gtgtctctcg ggcttttcat gagagcagtt ctgcagctct
      481 tcgaatgcgt cttagggttt cgtttttacc caatatcgac gcaagcgtaa atggatcagg
      541 cgatttttct cttcctgaaa gtgcaaaacg taacggccac aaaacattcc cttttccctt
      601 ctcctctgcg taggtccaga gtgccttgcg tactgtgtca gcatcgaaag ttttatcaag
      661 actttcgagc ttcgcagccg tttctgtaag ataaattttt gtgttatcgg tgtctttctc
      721 tcccttccag tgaagatctt cttttttata ggttggctga ctgaaataat ataaaagttc
      781 tccgttatcg gccagcttct taatgtcact caggacttct attcgttcaa aaactatcgg
      841 cacaattttc tttacgattt gatcatctgc ctctggaaac aattttctga cctcattgat
      901 aattgatatt tgataattgc catttttctt caggtgttcc ttgttgaacc atcgtagctt
      961 ttcttcactg aaaattgcgc ctgctttgtg aactctcgaa agatcaaatt cactgattaa
     1021 ttcatccata ttgaaaattt ccttatctcc tcccggattc catccgagaa gcgccaaata
     1081 atttaccaaa gcttcgctta gaaatcccat gtttttgtat tctgtaacag aaactgcgcc
     1141 gtgacgcttt gatagttttg acctgtcagg tgccaatatg agcggaatgt gcgcgtaaga
     1201 tggtctcgta aaaccgagcg cctcgagaat aagaatttgc cgtggagtgt ttgaaatacc
     1261 atcttcaccg cggatgacgt gcgtgactgc catttcatgg tcgtctataa cgacggctag
     1321 atgataaagc ggctcctcga gactcttggc gatgacgaaa tcaccgagct ccgtggtgtc
     1381 gaatgagacc tcaccccgta taagatcctg gaaaacaatt cttatgttgg gatttttaaa
     1441 acggattacc tttttactct tatcatcggc tgtgctttct gcctcgtatg ccgctccgct
     1501 ttcaagcaat tttgtaaatt gggcacgata aatatgcata cgttcagact gtcgcgctac
     1561 ttctgggagc gtacattcaa taccgagcca ttcgagtccc gcacgaatgt catcctcgta
     1621 ttcttttttg cttcgggcct tgtccgtgtc ttcgaagcga agatacagga cgccattatt
     1681 tttctttgca aaaagatagt taaaaagagc cgttcgtgcg cttccaatat gaaaaaaacc
     1741 tgtcggcgat ggcgggaagc gtgtaataac cttctcactt atgttttgaa gaggcatatc
     1801 acgtgatttt agcatatttc aagtgaacaa tccgaattag cattgacttt gagcaaagat
     1861 atgacatagt agaaacagac atgaattgaa catatgaaaa ccaccgccct taccttcgca
     1921 ccaacgtcag atgtcactct tcgtcaatgt ttcgccgtcg gcgctcgctc cgagaacacg
     1981 ggaattggaa ttccgcttcg catatttttc ccgctgttaa gtcagccgga gtatcacgtt
     2041 cttgctgttc cgggactctg ctatagtgag gaattatttt atccgtgggc tagcgcgctc
     2101 gcggcgtatg gaatcgagac tgccgtcatg gcatatgatc cgatgcgatc gcccaaggaa
     2161 aagacacttg cccgctgtgc caacgacatt gcatctgccg tcgcacatct aggccattct
     2221 cccctcgtga ttcttgctca ctccgcaccg gcgctcgcca tacgtctcct cactcccgag
     2281 caaaaaggca aacacacttg ggtgattgcg c
//
LOCUS       DATDSU010000156         7397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_330027, whole genome shotgun sequence.
ACCESSION   DATDSU010000156 DATDSU010000000
VERSION     DATDSU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330027"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="greA"
                     /locus_tag="VI981_05370"
     CDS             <1..346
                     /gene="greA"
                     /locus_tag="VI981_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4479754.1"
                     /translation="LGDLSENAEYHSAREAQAAMEERIMVLEEILKNAVIVGGKHDTD
                     AVGIGAVVKVRCGNDEKLYTVVGSEEADVVAGKISNKSPLGEAMMGKRKGDAFPYTTP
                     AGEKKCEILEIR"
     gene            463..1920
                     /gene="lysS"
                     /locus_tag="VI981_05375"
                     /pseudo
     CDS             463..1920
                     /gene="lysS"
                     /locus_tag="VI981_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
     gene            2033..5920
                     /locus_tag="VI981_05380"
     CDS             2033..5920
                     /locus_tag="VI981_05380"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4479755.1"
                     /translation="MIKVMPIRRLLKESFITMGKFTQEVAAGQEESREVRKALPELQL
                     TELQKSEKPINDIEGKKLKVEAVRPWNNYTIVTLEGSDEIQIFDEDARVIPEFTKPLN
                     SKKVKVEWASYSVAQKLGPRKGYIGEDTKNAPDMIKISEVPLELRGFARVFLATSSGG
                     EIKVSVMPATEFTASDVVVLDKYIILTTKEGGYFAYVTKNENGVVLAPQNWQRIDWSK
                     PVPPELNKYAERILGEHEGYKQLNDRYSALITDVGINIVENNKVKGAPVFSDLVPSVE
                     RNVAIDPTNPNVIYYCQSSNPRNIKRIDLSAEPNTWGVVAAELPQKYDSISNLQLDPS
                     GNFFLLYSKEDLVILTRDGLQEVKRVPKLTQVNFDAAGKIRAVDKDGRLAIYEPNFEE
                     LAQEIDRHRVEKLAAGIQLQDIFDLRASKKSEKGGEVLERLEPLRAKYQEQFGEVLAR
                     ITTQDGVRELRQGFNKLRDQLRRHGLKPNEVNFIIEGLEVPIVEKEKEFAVRETQEVL
                     TSVRAKLTSSLSITSISEARAEVDKIKAVEALLEGDLRQEFRQVVQEFEQKSLEFFHQ
                     RGGEVIKDVRGLLERTKTDLEAFTSKIQMDDWLEFRYPQLKSRLGSLAHDVPLEADEA
                     YKAIVSARTELQELATQFEEKFKREYAKVREKAVERIEGTVDTLESDIGGLIGRLRSK
                     GFADRRAAEQYLDTSEARRTLETEITALAGNNPDIAKELERALKVRLSNALTEIERGG
                     MTQVAETGQQMILFGETSFPKWEAKVKEKGERKVDLVFDEDEKTHGPGIKAGDILGDV
                     AINIKTSTGKAENVRPYEGWQDENEWRLGLFTYRGESIPPSYVTAADYKTIKKEYSDW
                     SLGERSKLQTELQEKRNTLKEIYSRRQKIGEKTPDVDSAWQEEYKAKLQEYATFVAEH
                     HIPLLKRIDRIKAEPEVEYTNGKGYVPEWQSHWVNDPQTEKDLEEMAKALKMQLDLQE
                     GMLNLKGHAGTGKDVRMKMFCALTNRPYFGIDGTKWTTEFELSEDVMLESKDGASQTV
                     KVPSAVLNGITTPGAVVYFNEFNAMPEQAQIFLHALMDEKRSLTLKTSSGKVIRANPS
                     VLLASSMNPGYPGTFDPQFATRSRMVSIEVGYPPLTRKPEAGDTNLNPPYDSSEALRI
                     ARGVDSLSDLTYEASLERNEFVKMWDKYVNGIENGSPDPNGTQKFDIDTSLALVQFAD
                     NLRENFIKIFEKSRDARNALPVSQPITGRELRRAAYELSKMTPEEKATANPEAIARNL
                     LDKYFLTHIDKKEDRDKIRTAMNTWTSKKRVRA"
     gene            5925..>7397
                     /locus_tag="VI981_05385"
     CDS             5925..>7397
                     /locus_tag="VI981_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479756.1"
                     /translation="MERPHEFKETPPQTRPLKLTREMLFLAEQAAEGKLKILPVKGWS
                     LHYPAEPDVRAEKLQGLLDGKYTAQEVARDIKPDALLYDAEDIQKEGLENVSARVRDL
                     SAFIAHYDYPRFAKFVESMRGRQIPVSELEGLYTEVTQARVQKKVMDAYGYTGKKQIE
                     NALRNEASGVIEGIKNLPRSQKVLKALKTNWLSEELGLVAPEERDRVVSELSGDERKV
                     FENLQSSYREYIQRGDENAYKELTKIIQEDFPKIQKQTESGQPSESMQELQKELEEFM
                     EQTVPPGSPEDPAIPPEDKDEYHTPPPSPGESKEKMQDRPIFEINPALGGYYASGRKS
                     YFDVDAKTWSKKKQLFPYSVSVTGKDRCTISGTLDSGVKSLPLPNGYALDTSSLKAKG
                     ANIEVRRDQNGCFYLEVGGTGSFSVDFLQEQNPFAGKMIPEDNAPLYRGSLSQKTETT
                     ISKLIGSPLQKAEQARQHILANHFYPGGGDLQMAQALQYKL"
BASE COUNT         2229 a         1613 c         1866 g         1689 t
ORIGIN      
        1 gctcggcgat ctttcggaaa atgccgaata ccattccgcg cgcgaggcac aggccgcgat
       61 ggaagagcgg atcatggtgc tcgaagaaat tttaaagaat gcggtgatcg tcggcgggaa
      121 gcacgacacc gacgccgtcg ggatcggtgc ggtggtgaaa gtgcgctgtg gaaacgacga
      181 aaagctctat accgttgtcg ggagcgaaga ggcggacgtc gtggcgggaa aaatttcaaa
      241 taaatctccg ctcggcgagg cgatgatggg aaagagaaaa ggagacgcgt ttccgtatac
      301 gacccccgcg ggcgagaaga agtgcgagat actcgaaatt aggtagtagg tggtaggttg
      361 tagtgagtag aaaaatgcga atgcgttgct tcgttttccc tactcactac ttactacccc
      421 ctactcacta cttactaccc cctactcact acccactacc ctatggcttc catcgaagaa
      481 ctccgaaacg aacgtttgaa aaagttggac gtgcttcggg cgcacgggat cgacccctat
      541 ccgtcaaaag tgccgcgcga cttttcgctc gaagagacac gcgcgaagtt caaagaactc
      601 gaaagcgaaa agaaaacgct ctcggttgtc ggccgcgtga cggccattcg cgggcaggga
      661 gccatccaat ttgtcgtact caatgacgga acggcgacat ttcaggcggt attcaaaaag
      721 gacgtgctcg gcgaggagct aatgcagata ttttccgaag ttgcggatat cggagatgtg
      781 gtcggtgtct cgggcgaact atttgtaacg gcgcgcggcg aagagagcat catggcgaag
      841 tcctggacga tggcggcaaa agcattgctc ccgcttcccg agaaatggca cggattgcag
      901 gatccggaag agaaactgcg caaacggtat ctcgaccttc tgcttgatcc cgaattgcgc
      961 gatcttttta taaaaaaagg aaaattttgg aatgcgatcc gcaacttcat gctctccgaa
     1021 ggttttgtcg aagttgaaac gccggtcttg gagaatacca cgggcggcgc cgacgcacgg
     1081 ccgttcaaaa cgcattacaa cgcgcttgat atggatgtgt atttgcgtat ttccgccgga
     1141 gaactttggc agaagcgttt gatggtcgcg gggttcaaca aggtgtttga gatcgggcgc
     1201 atttttcgca acgaaggtat gagtgcggag cacgcgcagg actatacgca actttaattt
     1261 tactgggcgt acgcgaatca cgaggacgga atgaaattcg tggagcgcat gtacaaagaa
     1321 gtggcacaag aagtcttcgg cacgcgcaaa ttcaccatcg gcgcgcacga ggttgatctc
     1381 ggcggaaagt gggagcgcta tgattacgcc gaaacgatca agaaattcac cgacgttgcc
     1441 atcagcacgg ccgcgctccc cgatatcgaa gccgccctcc tgcgccttaa tgtcgagtat
     1501 gacaagaagg ggtggaatcg cgtgcgcgcg atcgacgctc tttggaaatt cacgcgaaag
     1561 aaactcggcg gccccggatt tcttgtgaat gttcccgtcg aaatgtcgcc gctcgcgaag
     1621 cttgcgccgg ggggaaagac tgtcgaacaa tttcaaccga tcttggccgg aagcgaggtg
     1681 gggaaggggt acagcgagct caatgatccg ctcgatcaag ccgcacgatt tgccgaacag
     1741 gaaaaattgc gcgaaggggg agatgaggaa gcgcagatgt atgaccgcga ctttgtcgag
     1801 gcgctcgaac acggcatgcc gcccgcgtgc ggcttcggag tctcggagcg gctctttgcc
     1861 tttctcgcga accgctcggt ccgcgacgca cagctcttcc ccttaatgcg tccaaaataa
     1921 ttacttgggg gtcggacgtt ggataaaaac gtcatcccgc cttgtaccga gtgcaagttc
     1981 tcggccacgt cgccccctga acgtgtactt tgcgaaaagt tggggaggcg atatgatcaa
     2041 agtgatgccg atacggcgtc ttttaaaaga atccttcatc accatgggaa aattcacaca
     2101 agaagtagcc gcaggacaag aagagagccg agaggttcgc aaagctttgc cggagcttca
     2161 actgacagag ttgcagaagt cagaaaaacc cataaatgat atcgagggca aaaaattaaa
     2221 agttgaagct gtcagacctt ggaataatta caccatcgtc accctcgaag gatcggatga
     2281 gatccaaatt tttgatgaag atgctcgtgt cattcctgaa tttacaaagc cattaaacag
     2341 taaaaaagta aaagttgagt gggctagtta ctctgtagca cagaagcttg gcccaagaaa
     2401 aggatatatt ggcgaggata ctaaaaacgc cccggatatg ataaagatat ccgaagtgcc
     2461 tttagaatta aggggttttg ctagagtttt tttggcgacc agctctggtg gtgaaattaa
     2521 agtaagtgta atgccagcaa cagaattcac tgcatcagat gtggtcgttc tggacaaata
     2581 tatcatttta acaacaaagg aaggtggtta ttttgcttat gtcaccaaga atgaaaatgg
     2641 tgttgttctt gccccacaaa attggcagag aattgattgg tcaaagccag tgccaccaga
     2701 gctcaataaa tatgcagaaa gaatactagg agagcacgaa ggatacaaac aattgaatga
     2761 taggtattct gccctaatta ccgacgtagg cattaacatc gtcgagaata acaaggtcaa
     2821 aggagctcca gttttttctg atcttgtccc ttcggtggaa agaaatgttg ctattgatcc
     2881 gacaaatccg aatgttattt actattgcca gagcagtaat ccgcgcaaca tcaaacgcat
     2941 tgatttaagc gcagaaccca atacttgggg agtggttgcg gccgagcttc cacaaaaata
     3001 tgacagcatc agcaatttac agcttgatcc atctggtaac tttttcctgc tctactctaa
     3061 agaagattta gtaattctga cgagagatgg attgcaagaa gtaaaaagag taccgaaact
     3121 cactcaagtg aattttgatg cagcggggaa aattcgtgca gtcgataaag acggccgttt
     3181 agctatttac gagccaaatt ttgaagaact cgctcaggag attgataggc atcgagtaga
     3241 aaaattggca gcaggaatac aacttcaaga tatatttgat ctgcgagcat ccaaaaagag
     3301 tgaaaaaggc ggagaagttc tggaacgact agaaccatta cgagccaaat atcaagaaca
     3361 gttcggtgaa gtgcttgcaa ggataaccac acaggatggc gtccgggagc ttcgtcaagg
     3421 ttttaacaaa ctccgtgacc aattgcgtcg gcatggatta aaaccaaacg aagttaattt
     3481 cattatcgaa ggcctcgagg ttcctatagt agaaaaagaa aaagagtttg ctgttagaga
     3541 gacacaagaa gtacttactt ctgtacgagc aaaactcact agtagtctgt ctatcacttc
     3601 aatctcagag gcacgcgcag aggttgataa aatcaaagct gttgaagctt tactcgaagg
     3661 agatttgcgg caagaattcc ggcaagtcgt gcaggaattt gaacagaagt ctctggagtt
     3721 tttccatcaa agaggcggcg aagttattaa agatgttcga gggttactgg agagaacgaa
     3781 gacagacttg gaagcgttta cttccaaaat tcagatggac gattggttgg aattccgcta
     3841 tccgcaatta aaatcccgac tcggttcatt ggcgcacgat gttcccctgg aagccgacga
     3901 agcttataag gcaatagtta gtgcccgtac tgaattacaa gagcttgcta cccaatttga
     3961 ggaaaaattt aaacgtgagt atgcaaaggt tcgagaaaaa gcagtagaac gcatagaggg
     4021 tactgttgat actttagagt cagatattgg ggggttaatt ggtcgtttac gctctaaggg
     4081 ttttgctgac cgaagggccg cagaacaata tcttgacacc agcgaggctc gcaggacttt
     4141 ggaaactgaa ataacggctc ttgcaggaaa caatcctgat attgccaaag aattggaacg
     4201 cgctcttaag gtaagacttt ctaatgccct cacagagatc gagcgcggag gcatgaccca
     4261 agttgctgaa accggccaac agatgatttt gtttggtgag acctctttcc ccaagtggga
     4321 agcgaaagta aaggaaaagg gagaacgaaa agttgatctt gtttttgatg aagacgaaaa
     4381 aactcatggc ccgggcataa aagcaggaga cattcttggt gacgttgcca taaatattaa
     4441 aacctcaacc ggcaaagcag aaaatgttcg accatatgag ggttggcaag atgagaacga
     4501 gtggcgcctg gggctattta cttatagagg cgaatccatt ccaccgtctt atgtgacagc
     4561 tgccgattat aaaacaatca aaaaagaata ttcagattgg tcgttgggtg agcgatctaa
     4621 attgcaaacc gaactccaag aaaaaagaaa cactctcaag gaaatttatt cgcgaagaca
     4681 aaaaattggc gaaaaaactc cagatgttga tagcgcatgg caagaggaat ataaagcaaa
     4741 gctccaagag tacgctacgt ttgtcgccga gcaccatatt cctttactaa aacgcattga
     4801 tcggataaaa gctgaaccgg aggtcgaata tacgaacggt aagggatacg ttccggaatg
     4861 gcagagtcat tgggttaatg atccgcaaac agaaaaagac ttggaagaaa tggccaaagc
     4921 gctcaagatg caactcgatc tgcaggaagg aatgcttaac ctcaaaggac acgcaggtac
     4981 aggtaaagat gtgcgcatga agatgttctg tgcccttaca aatcgtccct actttggcat
     5041 agacggtaca aagtggacta ccgagttcga gttatctgaa gatgtaatgt tggaatcaaa
     5101 agatggcgca tctcaaacag tcaaggttcc ctctgcggtc ttgaacggaa tcaccacgcc
     5161 gggtgctgtt gtttacttta atgaatttaa cgctatgcca gagcaagccc agattttctt
     5221 gcatgcgcta atggacgaga aacgctcgct tactcttaag accagctctg gaaaagtgat
     5281 tcgtgccaat ccgagtgtat tgcttgcgag ctctatgaac cccggatatc ccggcacatt
     5341 tgatccgcaa tttgctaccc gaagtcgtat ggtctcgatt gaagttggat acccaccgct
     5401 tactcgcaaa cccgaagctg gagacaccaa cctcaatcct ccttacgact cttcagaagc
     5461 ccttcgtatt gcccgtggcg ttgattcgct ttccgatctc acctatgaag caagtcttga
     5521 acgcaatgag ttcgtgaaaa tgtgggacaa gtacgtcaat gggattgaga atggttcacc
     5581 agatccgaat ggcacacaga aattcgatat tgacacttct cttgcgctcg tacagtttgc
     5641 cgataatctc cgtgagaatt tcatcaaaat atttgaaaag agccgagatg cccgtaacgc
     5701 cctgccggta tcacaaccta ttaccggccg tgaattacgc cgagccgctt acgaactaag
     5761 caagatgact ccggaagaaa aagcgaccgc aaatccagaa gctattgctc gaaaccttct
     5821 tgataaatat ttcctaactc acatcgacaa aaaggaagat cgggacaaaa ttcgtacggc
     5881 aatgaatacc tggacttcca agaagcgtgt aagagcgtaa atctatggaa cgccctcatg
     5941 aatttaaaga aactccaccg caaacccgtc ctctaaagct aactcgtgag atgctatttt
     6001 tggcggagca ggccgccgaa ggtaaattaa aaattctccc cgtgaagggt tggtctttgc
     6061 attatccggc agagccagac gtgcgagcgg aaaaattgca ggggcttctc gatggtaaat
     6121 ataccgcaca agaagtagcg cgtgatatca aacccgatgc gcttctctac gatgccgagg
     6181 acattcaaaa agaaggacta gagaatgtca gtgcccgcgt cagagactta tcggcattta
     6241 tcgcacatta cgattatccg cgttttgcaa aatttgtaga gagcatgcgt ggccgacaga
     6301 ttccggtatc cgaacttgag ggtttataca ctgaagttac acaagctcgg gttcaaaaga
     6361 aggtaatgga tgcgtatggt tacaccggta aaaaacagat agagaatgca ttgcgtaacg
     6421 aggccagtgg cgttattgaa gggatcaaaa atcttccaag atcgcaaaaa gtcctaaagg
     6481 cactaaaaac caattggttg agtgaggaat tgggacttgt cgctcctgaa gagcgtgatc
     6541 gggtggtttc tgaactttcc ggagacgaac gaaaggtttt tgaaaattta cagagttcct
     6601 atcgcgagta cattcaaaga ggagacgaga atgcctataa agaactaact aaaatcattc
     6661 aagaagactt ccccaaaatc caaaaacaaa ccgaaagtgg ccagccaagc gagagtatgc
     6721 aggaactcca gaaagaattg gaagaattta tggagcagac cgttcctccg ggctctcccg
     6781 aagaccccgc cattcctccg gaagacaaag atgaatacca tacgccgccg ccatctcccg
     6841 gcgaatcaaa ggaaaaaatg caggatcggc cgatttttga aattaatccg gcactaggtg
     6901 gatattatgc aagcggccgg aaaagttatt ttgatgttga tgccaaaaca tggagcaaga
     6961 aaaaacagct tttcccatat agcgtctctg taaccggcaa agaccgatgc actatttccg
     7021 gaactttgga tagtggcgtt aagtctctac ctcttcccaa cggctatgcc ttggatacat
     7081 cgtctctaaa agctaagggg gccaatatag aggttagacg tgaccaaaat ggatgttttt
     7141 atcttgaagt tggcgggact ggatcattct cggtagattt cttgcaagag caaaatccgt
     7201 ttgcaggaaa aatgatccct gaagataatg caccccttta tcgaggatca ctttcacaaa
     7261 aaacagagac aactatttca aaacttatag ggtcaccatt gcaaaaagct gaacaagcac
     7321 gacagcatat tttagctaac catttctatc ccggaggtgg agatctacag atggcgcaag
     7381 ctttgcagta caaatta
//
LOCUS       DATDSU010000157         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1497612, whole genome shotgun sequence.
ACCESSION   DATDSU010000157 DATDSU010000000
VERSION     DATDSU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497612"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VI981_05390"
     CDS             <1..631
                     /locus_tag="VI981_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479757.1"
                     /translation="NPEVENDLTEDGVRQVQSLAARINSRLKGGEKVYVMKSPRVRAQ
                     NTAKLLKDELSQANHKVAELGKGRSSLDKIKDINSQGEEVLYVDGAKEKYLSEIAEIV
                     ARKNPDYYTELRAGKTENLVSEPIDKYREKVTSVIKRLVEIARKKAGENEILILSTHG
                     EWLDTVLEQYFSKKITKETQVKPASEIELQVFQDKIVFIYGDEKAEVNV"
     gene            646..1251
                     /locus_tag="VI981_05395"
     CDS             646..1251
                     /locus_tag="VI981_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479758.1"
                     /translation="MKFLKRVKTIEVKRCFVGSQYIRGGSDKRRVPTFERNEFIKKQN
                     QARKYVKRLSEKQLDRIIATEFKKRLMAYNDCDWHIGTINVNEVGVWKGAGGLPVYWT
                     ADSLKKTAVKVAWALSRPEKHRIRALKAIPRILKTSLDLLEQDPYSLPIVLHGGIGTA
                     GRKGLKKMKGDIDDGCIRAIALAISGKKNLKAYIGSVRHSV"
     gene            complement(1669..2979)
                     /locus_tag="VI981_05400"
     CDS             complement(1669..2979)
                     /locus_tag="VI981_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEY4479759.1"
                     /translation="MPRTGAKNVRVAFSAGQLTHFGGVYLLHRFLQQLQLRTLLSRKL
                     QIHERNNHFSTTERLFALLYPMILGLDKIELTALLGTNGVFQYLTGLPRVPNPTTLRR
                     FLISNADILLPKLRSAHNVLRAHFLTLPESHSSYWLDFDSTARTLYGNQEGAAKGYNP
                     RHPGKKSYHPLICTEAHFQDCLGGELRYGNAHTAEGANDMLNTVLAALPTLTRTIRTR
                     ADAGFYDGDFIQQLCDNRVEFAVVARMTAPLKNTIPALRYTKVGSVYSAAEFQYQPHG
                     WKQKYRFVVLREQLTDKRDAQLTLFKMKAYAYNVIVTNLSLTPHGVFAFYEDRSAIER
                     VIRTLQEDYPFGTAPTGNFAANALYAELSLLAYNIVIWFRRLCLPDDWQSYTVQTLRH
                     RFLLIPGVFTRTDNRPVLKLPKNNPHQNTFLYAQDRIKKLKSLV"
     gene            3709..3924
                     /locus_tag="VI981_05405"
     CDS             3709..3924
                     /locus_tag="VI981_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEY4479760.1"
                     /translation="MRTIINISLPEKMAEEVEAEIKKERFSTKSEFFRMLVRLWLEKE
                     LSEDLEESRNEFRHGKGKLLRSLKELR"
BASE COUNT         1223 a          750 c          924 g         1033 t
ORIGIN      
        1 caaccccgaa gtagaaaatg acttaacaga ggatggagta agacaggttc agagtttagc
       61 tgcccgaata aattctcgcc ttaagggtgg agaaaaagta tacgtgatga aaagcccaag
      121 agttcgcgca cagaataccg caaaactttt aaaagacgaa ttatctcaag caaatcataa
      181 agtggcggaa cttggcaaag ggcgttcatc gttagacaaa ataaaagata taaattcaca
      241 aggagaggag gttttgtacg ttgatggcgc aaaagaaaag tacctctcag aaatagcaga
      301 aattgtggcg agaaaaaatc ccgactatta cacagaatta cgcgcaggaa aaacagaaaa
      361 tctcgtgtct gaaccaatcg ataaatacag ggagaaagtt acatcagtca ttaaaaggtt
      421 agttgaaatt gcacgaaaaa aagccggaga gaatgaaata ttgattcttt ctacacacgg
      481 agagtggtta gatacggttc tagagcaata tttcagtaaa aaaattacta aagaaacgca
      541 ggtaaaaccc gcgagtgaaa tagagctgca ggtttttcaa gacaaaatag tatttattta
      601 tggagatgaa aaagcagagg ttaatgtata agtatcgtac aagctatgaa gtttctcaaa
      661 agagttaaaa cgattgaagt caaacgttgt ttcgtagggt cgcaatacat acgaggcggg
      721 tcggataaac gacgggtacc aacattcgaa aggaatgagt ttataaaaaa acagaatcag
      781 gctaggaaat atgtaaaaag actttccgaa aaacagctag atcgcatcat tgccacggaa
      841 tttaagaagc ggcttatggc ttacaatgat tgcgattggc acataggaac tataaacgtc
      901 aatgaggttg gggtttggaa aggggccggg gggttgccag tttattggac cgcagactca
      961 ctcaaaaaaa cagcggtaaa ggtagcgtgg gccttatcga gaccagagaa acatcgcatt
     1021 agagcattga aagcaatacc aagaatttta aaaacatctc tcgatcttct ggaacaagat
     1081 ccgtattcgt taccgattgt tttgcacggc ggcataggta cggcgggcag aaagggatta
     1141 aaaaaaatga aaggcgatat tgacgatggc tgtatacgag caatagcgct tgcgataagc
     1201 ggcaagaaaa acttaaaagc ttatatcggc tctgttcgcc acagtgttta attttggggg
     1261 aagaaaatac gaatagccgc aaaagaatac aaacaaagaa cgacaaccag tcgttctttg
     1321 tttgtaaatc acggagcata ttcggcaatc acgatattca cggagctttc gggttgccgc
     1381 ttcagcattg ttggcacatt caaaaagtca taaatgatcg tacagcggtt gccgacggca
     1441 caatattcca ttattgctca agcgtttttt ggttggcaat gaacttttcc cgatattcca
     1501 caagcgcctt tctcagtgat acaatatcgg tcgtaatatt cccgccgctt tttctgataa
     1561 agtgttccac ctgtagatta acaacttaaa tcctgcgtga aaatgaccaa ttttttcgct
     1621 tgcagaatca ggacaatttt ccggaacgac atttgagaat tgtcccgatt acacgagcga
     1681 tttcaatttc ttgattcggt cttgcgcata aagaaacgta ttctgatgcg gattgttctt
     1741 tggtaacttc aataccggtc ggttgtcggt tcttgtaaag acgccgggaa taagcaggaa
     1801 acggtgccgg agcgtttgaa ccgtatatga ttgccaatca tctggcaagc agagccgccg
     1861 aaaccaaatc acgatgttgt aggcaagaag cgacaactcc gcatacaacg catttgccgc
     1921 aaagtttccg gtaggcgccg ttccgaaagg ataatcttcc tgtaaagtac gaatcactcg
     1981 ttctatggca gagcgatctt cgtaaaacgc gaatacaccg tggggagtca gggaaagatt
     2041 ggtgacgata acgttgtagg cgtatgcttt catcttaaaa agcgtcagtt gcgcatcgcg
     2101 tttatccgtc agttgctcac gcagaacgac aaaacggtat ttctgtttcc atccgtgagg
     2161 ctgatactga aattcagcag cagaatacac agaaccaacc ttggtgtatc tcaaagcagg
     2221 aatcgtgttt tttaatggag cggtcatgcg tgctacaacc gcgaattcaa cccgattatc
     2281 gcataattgc tggataaaat cgccgtcata gaatcccgcg tcagctcggg tacgaatggt
     2341 ccgtgtcaag gtcggaagtg cggccaacac cgtattcaac atatcattcg ctccttctgc
     2401 cgtatgcgcg tttccgtatc gaagttcgcc tccaagacag tcttggaaat gagcttcggt
     2461 gcagatcaac ggatgatagg attttttgcc aggatgacga ggattgtatc ctttcgcggc
     2521 accttcctga tttccataca atgtccgagc tgtagaatca aaatcaagcc agtaactgga
     2581 atgcgattcc ggcagagtca gaaagtgggc gcgcaggaca ttatgggcgg agcggagttt
     2641 tggaagaaga atatctgcgt tgctgatcag aaaacgtctg agcgttgtcg gattcggaac
     2701 acgcggaagc ccggtgagat attggaatac gccgtttgtt cccaacagtg cggtcaattc
     2761 gatcttgtcg agtccgagaa tcatcgggta aagtaacgcg aataggcgtt cggtggtgct
     2821 gaaatggttg ttgcgttcgt gaatctggag tttgcggctt aacaatgtgc gcaattggag
     2881 ttgttgaaga aaccgatgga gcaggtatac accaccaaag tgcgtcagct gtccggcgga
     2941 aaaagcaacg cgaacatttt ttgcaccggt tcttggcata tacatgaacc taattaaaaa
     3001 ttagcttcat gtatatgtac catagtggag aattttgtct attttaaagg tcggaaatga
     3061 atgttttggt cattttcacg caggatttaa gtccctaaat cattccgctt tcggcagaat
     3121 agtcgggcga tcaggttcgt tgtacgaatc tgatgtatcc tctattactt gagttcgaag
     3181 atcgtccact aagtagctgt gaacaggaac accgccttat gggcggtgtt ttaatttacg
     3241 ggtggggaga attagaaccg cttgattttt tcgaatctct gcgttattat tacttcagtt
     3301 ctatttcatg aaggaggaaa aaaatgactt ggttgctcat agtaggcgcc tcattattgg
     3361 cagtatactt gctcatcgga ctactttttg taaggcttat taacagttcg acaagaggga
     3421 agatgaccaa aaaggaaaaa atatttgtat ttttacattg gtaccagtac ttcatgcgac
     3481 aatcctaatt ttcgccgccc accagaacgg ctttttttat tcaaaacttg gtatcaaaaa
     3541 aggctcattt ttctcaaaac gtagtgcact aacccgtttt gagtgaaaac accgcatcag
     3601 gtgcggagtt ttcttttgag tacaaaaaag gaatttgatg cctattgaca tgtatgtgat
     3661 atcagctata attgtaatat aaattattac aattaatttc gttgaattat gcgtactatt
     3721 ataaacattt cattgcccga aaagatggct gaagaggtag aggcagagat caaaaaagaa
     3781 cgtttttcta caaaaagcga gttctttcgc atgctcgtgc gattgtggct cgaaaaagag
     3841 ctctcagaag accttgaaga atcaagaaat gaatttcgtc acggcaaagg aaaacttctg
     3901 cgttccttaa aggaattgcg ataactaact
//
LOCUS       DATDSU010000158         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1158013, whole genome shotgun sequence.
ACCESSION   DATDSU010000158 DATDSU010000000
VERSION     DATDSU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158013"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1314)
                     /locus_tag="VI981_05410"
     CDS             complement(139..1314)
                     /locus_tag="VI981_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479761.1"
                     /translation="MKSKRLYIYLAVIALAIVFFAVFTRKSANDVSSTIASETEEAVP
                     KNAENFLTNEKRNILSENPLNNSETPPQSETQKTDPCASKNNDEKLSCFGTYYGTMVH
                     TKGVKTAFDDLKARYDTDAYVKSECHQLIHLIGRAAFTKFNSVPKAFAVGDPVCWSGY
                     YHGVMEGLGLKDGLGAFSKENVDAICFEIPGKKTYSFEYYNCVHGLGHGIMAITYNKL
                     FDSLPFCDNLKGSWEQQSCGGGVFMENVIADSRDHKTAYLKPEDPVYPCNAVPEKHKQ
                     MCYLMQTSYMLKVNGRDFKKTFEICGTVDANYVDTCYQSLGRDASGQTTSDKDQTRSL
                     CMLGTGERQQSNCVMGAVKDFVSYFHSDTQALEFCNSLETNLASICRDTVKSYYRTF"
     gene            1458..2357
                     /locus_tag="VI981_05415"
     CDS             1458..2357
                     /locus_tag="VI981_05415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY4479762.1"
                     /translation="MTIYTHADRNVRRTIVLMSVFLVLIVALGWFFSYVFDNPSILVI
                     AVILSIAMNVGGYWFSDKIVLSLSGARLVNSEEHKDLWNIVENLSITAGLPMPKLYCI
                     DDSVPNAFATGRNKEHAAVAVTTGLLSLLERSELEGVIAHELSHVGNKDMLLSTIVVV
                     LVGFITLLSDFFLRMNYLGGRRDKNSGNVGLVLMLVGVLLAILSPLIATLIQLAISRK
                     REYLADASGVLLTRYPDGLAKALEKIGAVGGKMHRANHATAHLFISNPFGSVKERQSF
                     FTRIWSTHPPIEERVKILREMNK"
     gene            2459..3037
                     /locus_tag="VI981_05420"
     CDS             2459..3037
                     /locus_tag="VI981_05420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY4479763.1"
                     /translation="MLPEQQPTVADLIIKKTLLWAVPQWVKPNWITVLRFMTIPVVLY
                     LLVAEYYWSSIILFTISAFSDAVDGAIARTRNLITDWGKVYDPFADKLLIGTVTAIIV
                     TRYISPYLAMVILVIDLLLIINALYKKNVQHKVIQALFAGKMKMMLQSVGLWLLLANL
                     VVTSSALVLLATYLLYVAVLFALASLIVYRSV"
     gene            3142..3226
                     /locus_tag="VI981_05425"
     tRNA            3142..3226
                     /locus_tag="VI981_05425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3176..3178,aa:Ser,seq:gga)
BASE COUNT          878 a          704 c          760 g         1014 t
ORIGIN      
        1 tatattttgt cttcgctcgg acattgtgct aactgagagg tttgcacccg ctcctcgcta
       61 gccaaaataa attaccgcat tattttagac caatggaaat tgaaattact aaagtaaagc
      121 taaaagaagt gtgcaaaatt agaatgttcg atagtaggat tttacggtgt ccctgcaaat
      181 ggaggctaga tttgtttcga gggaattgca gaactcgagt gcctgtgtat cgctgtggaa
      241 atatgaaacg aaatctttaa ctgcgcccat gacgcaattt gactgctgac gctcgcctgt
      301 cccaagcatg cagagtgaac gagtttggtc cttgtcgctg gtggtctggc ccgatgcatc
      361 acgtccaaga ctctgatagc acgtgtccac atagtttgcg tcgacggtgc cgcagatttc
      421 gaatgttttc ttgaaatcgc ggccgttaac cttgagcata tatgaggttt gcatcaagta
      481 gcacatctgt ttgtgttttt caggcacagc gttgcatgga tatacgggat cttcaggctt
      541 taaatatgca gttttgtggt ctctcgaatc agcaatgaca ttttccatga agactccgcc
      601 gccgcaggat tgctgttccc acgagccttt taagttgtca cagaatggaa gcgagtcgaa
      661 taatttatta taggtgatgg ccataatgcc gtgtccgagg ccatgtacac aattgtaata
      721 ttcaaatgag tatgtctttt ttccaggaat ttcgaagcag atcgcgtcaa cattctcttt
      781 tgaaaaagct ccgaggccgt cttttaagcc aagtccctcc ataactccgt gataatagcc
      841 tgaccagcac acagggtcgc cgacggcaaa ggcttttggc acggaattaa atttggtaaa
      901 tgccgcacgt ccgataagat gaataagctg atgacactcg ctttttacgt aagcatcggt
      961 gtcgtaacgc gccttgaggt catcaaaagc ggtttttaca cccttggtgt gaaccattgt
     1021 cccgtaatag gttccaaaac agctcaactt ttcgtcattg tttttactgg cgcagggatc
     1081 tgttttttgt gtttcagatt gaggcggtgt ttcgctgttg ttcaatgggt tttccgagag
     1141 aatatttctc ttctcattgg tcaaaaagtt ctctgcgttt ttcggcactg cctcttcggt
     1201 ctcgctggcg attgtcgagg agacgtcgtt tgcgctcttt ctcgtgaaaa cagcaaaaaa
     1261 tactatggcg agggctatca cagccaagta aatgtataaa cgtttagact tcatgtattt
     1321 caaattatca tattgtgtag acgaagtcac atttgagttc ctcttcactt ataaaataga
     1381 aacgatcgtt tctattttat aaaggttttt ttgtatgcct acattctatg gtgatataag
     1441 gtatactttt taatgtaatg acgatctata ctcatgccga ccgaaatgtc cgaaggacca
     1501 tcgtactgat gagcgtattt ctggtgctta tcgttgcgct cggctggttt ttttcgtatg
     1561 tgtttgataa tccgtcaata ctcgtaatcg cggttatact aagtattgca atgaatgtcg
     1621 gcggatattg gttttctgac aagatcgtgc tctcactttc cggtgcgcga cttgtaaatt
     1681 cggaagagca caaagactta tggaatattg ttgaaaatct ctctattacg gctgggttgc
     1741 cgatgccaaa gctctactgt attgatgact ctgtaccaaa tgcattcgca accggtcgga
     1801 acaaagagca cgccgcagtt gccgtgacga caggactgct ctcgcttctc gaacgctcgg
     1861 agcttgaagg agttatcgcg cacgagcttt cccatgttgg aaacaaggac atgcttttgt
     1921 ccacgattgt cgtggtttta gtcggtttta taacgctttt atcggacttt tttctaagaa
     1981 tgaactatct aggaggtaga cgggataaaa acagcggcaa tgtcggactt gtccttatgc
     2041 tcgtgggggt acttcttgct atactatctc cattgatagc aacgcttatt caacttgcta
     2101 tctcacgaaa gcgggaatac ctcgcagatg cgtctggagt tttgctcacc cgctacccgg
     2161 atggtctcgc aaaagcgctc gaaaaaatag gggctgtcgg aggaaaaatg cacagagcca
     2221 accatgcaac agcgcacctc tttatctcga acccgtttgg atcggtgaaa gaacgccaaa
     2281 gtttttttac gcgaatttgg agcacgcatc cacccataga agagcgggta aaaattttaa
     2341 gggaaatgaa taagtaaaaa ttatcaagtt ttataaaatt ataatcacca atcttataat
     2401 tgatctaaca aaaccgtccg aacaaatatc cattcggacg ggttgaaaat taaattttat
     2461 gctccccgaa caacaaccaa cggttgcgga tctcattatc aaaaaaacac tgttgtgggc
     2521 agtgccgcag tgggtaaagc caaactggat taccgtgtta cgttttatga ctataccggt
     2581 ggttctctat cttttggtgg ctgaatatta ttggtcttca attattttat ttacaatctc
     2641 ggccttttca gacgccgtgg acggcgcaat tgcgcggacc cgcaatttga tcactgactg
     2701 gggaaaggtt tacgatccgt ttgccgataa acttctgatt ggcacagtaa cggccattat
     2761 cgtgacgcgc tacattagcc cttatcttgc catggttata cttgtgatcg accttttgct
     2821 tattataaat gcgctctaca agaaaaatgt ccagcacaaa gtgattcagg ccctttttgc
     2881 cggcaagatg aaaatgatgt tacagtccgt tgggctgtgg cttctactcg ccaacctcgt
     2941 tgtgacaagc tcggcgctcg ttctgctcgc aacgtatcta ttgtatgttg ctgtgctttt
     3001 tgctctcgcc agtcttattg tgtatagatc agtttaaaga agacgctgtt agctgttagc
     3061 ttttagcgta taggttaagg ctcttgaagc aaaatcaaat tacgattaaa gttaaagtcc
     3121 gcgaaagcgg atttcgtttt tggagggttc gcatagcggt ctagtgcacc acgttggaaa
     3181 cgtggcgtgg ggaaacccac caagagttcg aatctcttac cctccgcaat tcaggatttc
     3241 gcaagctgtg gacgaatgtc tcgcggcgcg aaccatattt tggaagggcg aaagaaattt
     3301 tttatcaatt ttataaagtc taaataaata attcataatt ttttgaaaac aaatat
//
LOCUS       DATDSU010000159        10082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1500387, whole genome shotgun sequence.
ACCESSION   DATDSU010000159 DATDSU010000000
VERSION     DATDSU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500387"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VI981_05430"
     CDS             <1..294
                     /locus_tag="VI981_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY4479764.1"
                     /translation="DAISGSRPGARRDSVENYLKRLEDLEAIAKSFKGVEKSYALQAG
                     REIRVFVTPTEISDLDAQNMAREIALKIEKELKYPGEIKVNVIRELRSIEFAR"
     gene            640..912
                     /locus_tag="VI981_05435"
     CDS             640..912
                     /locus_tag="VI981_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY4479765.1"
                     /translation="MNKQGIAEAVHGVLGGTKVQAEQAVDAMIDSIVSTLKKGDEVSI
                     AGIGIFSVKTRAARQARNPRTGEAISVPAMKVPKFRAAKALKDAVK"
     gene            1247..1471
                     /locus_tag="VI981_05440"
     CDS             1247..1471
                     /locus_tag="VI981_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479766.1"
                     /translation="MTTIKQRINITANQEVERALKNAAKRDGVPVATKAAELLSIALE
                     IEEDTYLGSIANERALTDRSLFVHHDRAWK"
     gene            1452..1754
                     /locus_tag="VI981_05445"
     CDS             1452..1754
                     /locus_tag="VI981_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY4479767.1"
                     /translation="MTEPGNKAGVIVRYHPAVISKDIPALDRTAQKRIRLAINNKLLV
                     NPLLYGKPLHGTLRRFFKLRIGNWRIVYEITPSYVDILLIAHRKDSYSKALNRIGK"
     gene            complement(2252..2322)
                     /locus_tag="VI981_05450"
     tRNA            complement(2252..2322)
                     /locus_tag="VI981_05450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2288..2290),aa:Gly,seq:tcc)
     gene            2405..3499
                     /locus_tag="VI981_05455"
     CDS             2405..3499
                     /locus_tag="VI981_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY4479768.1"
                     /translation="MLYAFLILKTFLISVLFSFVPLQDLPPNTLSEITNTEPFRSYEE
                     VTPLEIKRQPPIAVKTTEIQLPNASAKKDDSPAATPTSTGIPMQELNLKTKAALVNIF
                     CVGIGKIEYSISGTGIFISENGVVLTNAHLAQYLLLASEKERFFDCVLRTGSPAVPTY
                     RARILYLPPQWVKDHPSHLSEARPTGTGENDYALLLVTRRVDGRPLPDKFPYISPDPR
                     VQLYPGNVVLEAAYPAGFLGNEFITKNLNSGSSIAHVVDVLSFDGQSRDFAILEGTVV
                     AQQGSSGGAIVGPDGTQVGLIVTLSDGKTTSERKLGIITTFHIARTIEKFTGQSLASF
                     ATESEVKADKFEETESPALITILKTALNLE"
     gene            complement(3486..3791)
                     /locus_tag="VI981_05460"
     CDS             complement(3486..3791)
                     /locus_tag="VI981_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEY4479769.1"
                     /translation="MVKKFTKKIEDFNCDNCGTTVKGTGYTNHCPACLYSKHVDVNPG
                     DREAICGGFMKPVELSSEKGKFVLTFECQKCQVKKRNKMAADDNFDEALKVKAPTRD"
     gene            complement(3887..3957)
                     /locus_tag="VI981_05465"
     tRNA            complement(3887..3957)
                     /locus_tag="VI981_05465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3923..3925),aa:Pro,seq:tgg)
     gene            complement(4042..4437)
                     /locus_tag="VI981_05470"
     CDS             complement(4042..4437)
                     /locus_tag="VI981_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY4479770.1"
                     /translation="MKQLTEKRIIGNKGEDIACKYLISRGFSIIERNYLRKWGEIDII
                     AKFKKFHFIEVKTVSRTNLRDMSAQKTSYRPEDNMHPWKLERLSRTIQTYIAEKAIAE
                     DVWQFDVVAVYLDLDRKEAKVEYLEDIVL"
     gene            complement(4441..4857)
                     /locus_tag="VI981_05475"
     CDS             complement(4441..4857)
                     /locus_tag="VI981_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4479771.1"
                     /translation="MKLAHIKLQVGVKILLKNKTGQFLLLRRSAEKYPKTDGVWDIVG
                     GRINIGVDLINNLRREIKEETGLEYQGEPLLIAAQDILRPDKHVVRLTYQGEIEGVPV
                     LNDESSECKWASISEMEQMKDLDVFVAELIQKNLVM"
     gene            complement(5071..5141)
                     /locus_tag="VI981_05480"
     tRNA            complement(5071..5141)
                     /locus_tag="VI981_05480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5107..5109),aa:Gly,seq:gcc)
     gene            complement(5352..5426)
                     /locus_tag="VI981_05485"
     tRNA            complement(5352..5426)
                     /locus_tag="VI981_05485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5391..5393),aa:Arg,seq:tcg)
     gene            5484..6992
                     /locus_tag="VI981_05490"
     CDS             5484..6992
                     /locus_tag="VI981_05490"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY4479772.1"
                     /translation="MTTKPPLLFLNIPQEVVNIVQMIAEKGFEAYLIGGCTRDLIIGR
                     KPKDWDITTNARPDQIIEIFPKTFYENTYGTVGVVNENGEDPSTKIVEVTPYRLEASY
                     SDKRRPDSIEFSEKLEDDLKRRDFTINAIAIQLRTKKNDAWACEVVDLFGGIKDLKNG
                     LVKTVGDPRERFFEDALRVLRAIRIAVELDFEIETETEKAIAEYATHLTHVSTERLRD
                     EFTRILMSDKPSKGIKMARNMGILKFIAPELEAGIGIKQNQAHLYDVWEHLLRTVDHA
                     AKKTWSLDIRLAALLHDISKPATRVWSDEKKDWTFYGHEVVGAKVAKDLLTRLKYPKE
                     TIDKVIKLVRWHMFFSDTEQISLSAVRRMIRNVGPENVWDLMNVRICDRVGIGRPKED
                     PYRLRKYKSMVEEALRDPISVGMLKIDGKKIMELTQEPPGPKVGHILHALLEEVLEDP
                     TKNSVEYLNSRVQELSKLETSELKRLGESGKERKDTEDEKKLDEIRKKYWVR"
     gene            complement(7313..8077)
                     /locus_tag="VI981_05495"
     CDS             complement(7313..8077)
                     /locus_tag="VI981_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY4479773.1"
                     /translation="MQILCWNVNGLRALSKKGQFDWLVNTGAEIICLQETKSEKEQLL
                     PAIVNPPGYHSYFDHSKGRKGYSGVATYSKIKPEKIVYEMGVETLDKEGRLIAVFYSD
                     FVLLNVYFPNGGGGPERLKYKLDFYEAFLTYAELIRKQNPKLIICGDVNTAHEEIDLA
                     RPKENETRTGFLPVEREWLDEVLNHGYVDVFRYFNPGKVVYTYWDTYTFARERNVGWR
                     IDYFFITPNLLARVKKIDILSNVYGSDHCPILLELK"
     gene            complement(8126..9070)
                     /gene="xerA"
                     /locus_tag="VI981_05500"
     CDS             complement(8126..9070)
                     /gene="xerA"
                     /locus_tag="VI981_05500"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEY4479774.1"
                     /translation="MEEKSEVKNLLRNFLEYLEIEKGRSLKTVENYERYLNRFFGFTR
                     IDKVKNITDDAIRDFRLWLNRQPSGQKLHGEEETLKKKTQNFHLIALRAFLKYLAKRN
                     ISALAPERIELAKVAERSLDLLDLEALDKILKAASGSDVRSLRDKAILELLFSTGLRV
                     SELCSLSREINLKSDEFSIRGKGEKVRVVFVSERAKAAVKAYLEARRDMDDALFVQVQ
                     GPIKKDLSKVVSLRLTARSVERIVKHYAIKAGISKKVTPHVIRHSFATDLLQNGADLR
                     SVQMMLGHANIGTTQIYTHMTDRHLREIHKNFHGKKRK"
     gene            9167..9436
                     /gene="rpsO"
                     /locus_tag="VI981_05505"
     CDS             9167..9436
                     /gene="rpsO"
                     /locus_tag="VI981_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY4479775.1"
                     /translation="MLTKRKKERVIKETQVHDKDTGSPEVQVAILTKRIDELAKHLKT
                     HTTDKHSRRGLLQMVANRQTHLGYLQKKNVRRYNALVKKLELKKK"
     gene            9497..>10082
                     /locus_tag="VI981_05510"
     CDS             9497..>10082
                     /locus_tag="VI981_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY4479776.1"
                     /translation="MAVIKHKEQRVGIFIDTQNLYHSAKNLYHAKVNFGQIVKDALAG
                     RALIRAVAYVISTESGEERGFFEALGKVGIETKVKDLQIFGSGAKKADWDVGLAVDAI
                     KMAPKLDTVIILSGDGDFIPLVEYLQMNEGCQVEVVSFGKSSSQKLKEACDEFLDLDQ
                     NPRKYLLGQYNRTRRPRIQASNHTNSDSSNIPTVT"
BASE COUNT         2955 a         2231 c         2120 g         2776 t
ORIGIN      
        1 gatgccatct cggggtctcg acccggagca cggcgcgact cggttgaaaa ttacctgaag
       61 cggcttgagg atctggaggc catcgctaaa tcatttaaag gagtggaaaa gtcttatgcc
      121 ttgcaggcag gccgagaaat ccgtgtattt gtcacgccga ccgaaattag cgatctcgat
      181 gctcaaaaca tggctcgaga aatagcgctt aaaattgaaa aagagcttaa atacccaggt
      241 gaaatcaaag taaatgtcat acgagaacta cgttccatcg aatttgcgag ataaaggagc
      301 gagattcttg aagatcacag ccgatttatt tcgagatttt ggcgaaaatt acatgaaaaa
      361 ggcaaaaaat aggattcacg agcatttttt cctcaaagat tacaacaagt atcgattgtc
      421 ttgtcaattt ttcaaccaaa atttgacaca ttaccggaaa aacgttcctc cacatcagcc
      481 gattgcctaa aaaatgttgt ctactataat tatttgcagg ttattgcaag ccgttctata
      541 gaaagagggt ttgcaaaatc cggtagtgac cgagcatttt gagctcaaag ttctcggctg
      601 ctatcgggag gagatattac taactaattc atttttacaa tgaataaaca aggtatcgca
      661 gaagctgtcc atggagtatt gggcggaacc aaggtccaag ctgaacaggc agtcgacgct
      721 atgattgact caatcgtcag cactctgaag aagggtgatg aggtgtcaat tgctggaatc
      781 ggtattttct cggtaaaaac acgagctgcc aggcaggctc ggaatcctcg aacaggtgag
      841 gcaatttcag ttcccgcaat gaaagtgcct aagtttcgag cagcaaaggc tctcaaagac
      901 gcagtgaaat aacttagcaa ttgtttcggc gcatttcttc gttggaggct ccattcgctt
      961 cttacagaag ctgtacacct ttcgcttccc gtcagacggg acgctcaagg tcttgcggtg
     1021 ctccaggtgt tcgcacctca cccgcgcagt atattcatac agcttggtcg cgatactcgc
     1081 ctccgcctcg aactgcatcc aaaccaattc gctaagtagc gccatcacga aaccacttga
     1141 tttccttaaa tcaacacaaa aaccgccata gggcggtttt tgtgtttgcg agccccaaat
     1201 cctcgcccct cttgactggt gatacacttt tgatacactt ttatatatga ccactataaa
     1261 gcaaagaata aatatcacag ccaaccaaga agtagagcgg gctctaaaaa atgcagcaaa
     1321 aagagatgga gtacccgtag ccacgaaggc agcggagctc ttgagtattg ctcttgagat
     1381 agaggaggat acttatctag gcagtattgc aaacgagcga gctctgacgg atcgctccct
     1441 cttcgtacac catgacagag cctggaaata aagcaggtgt tattgtgcgg tatcatccag
     1501 cagtaatcag taaagatatt cctgcattag accgaacggc tcaaaaaaga atacgcctag
     1561 ccataaacaa caagctttta gttaatcccc ttctctatgg caaacctctt cacggcacac
     1621 ttaggcgctt ttttaagtta cgaattggta attggcggat tgtatatgag atcacgcctt
     1681 cctatgtgga tattctactt attgctcatc gtaaagattc ctattcaaaa gcattaaatc
     1741 gaatcggaaa gtaattatgt gagtctgacc cgattcctga aacgacaagc gatactgcgg
     1801 ggcggtgcga aggtaatgta gcacacatga gagcgtctga aacatggcgt tcttttttat
     1861 ttcttggctt ttgtcaagtt gtcaagggct tgtcaagggc agtccttgac atttaattga
     1921 tgggggcgag tcgagggata gaattttttg attcaagggt gctcatcaga cttttgaatc
     1981 agcaaaaacc gccttaggcc gaaccgctag gttttaggct agcggttttt gcgttgtaga
     2041 caatcgatac ggggactgtt tttatttatg cgggacgggg gaatcggctt cgactcaaaa
     2101 ccttgccgtc gcaaagtcct ggtttagcca ccgactcgcg tccgaacttt gctaagttcg
     2161 gacttcgtgt gctccgccgt tcgactctcc cctttgccgt ctcggcaaaa caaaaatact
     2221 agacatcatg tttagtgttt ttgttttcgt gtgcgggatg ggagaatcga actcccgccc
     2281 ggagtttgga aaactcctat tctaccatta aactaatccc gcaggtgctg atgtcacatc
     2341 atagcagaaa ttttttttct tcaaaatacc catgacgact cactttttgt cgtataattc
     2401 tcctatgctc tacgcctttc ttatacttaa aacgttcctg atctcggttc tattttcctt
     2461 cgtgccgctt caagatttac cgccgaatac acttagcgaa atcacaaata ccgagccctt
     2521 ccgctcatac gaagaagtga cgccacttga aataaagagg cagcctccaa ttgcggtcaa
     2581 aactacagaa atacagcttc caaatgcgag tgccaaaaag gacgactctc ctgccgcgac
     2641 tccaacttcg acgggaattc cgatgcaaga gctcaattta aagaccaagg ccgctcttgt
     2701 aaacattttc tgcgttggca ttggaaagat agagtactct atctctggaa caggtatttt
     2761 tatttcggaa aatggagtag tgctcactaa tgctcatttg gcgcagtact tgttgctggc
     2821 ttccgaaaaa gagagatttt tcgattgtgt ccttcggacc ggcagtcccg ctgtcccgac
     2881 ttatcgcgct cgaattcttt acctgccacc tcaatgggta aaagatcacc catcacatct
     2941 ctccgaagca cggccgacag gaacgggaga aaatgattat gcacttcttc tcgtgacaag
     3001 acgcgtcgac ggtaggcctt tgcctgataa atttccgtac ataagtccag atccgcgcgt
     3061 acaactttat cccggcaatg tggtgcttga agccgcctac cctgcaggtt ttctcggaaa
     3121 tgagttcatt acaaaaaatt taaatagcgg ctcctctatc gcacatgttg tagatgtcct
     3181 tagttttgac ggccaaagcc gagattttgc catacttgaa ggcacagtcg tagcccagca
     3241 aggttcatcg ggcggagcta tcgtcggtcc cgatggtacg caagtaggtc tcatcgtgac
     3301 tttatcggac ggaaagacga cgtctgaacg caaactcgga atcataacaa ctttccacat
     3361 cgcgcgaaca atagagaaat tcaccggtca gtcgcttgcc tcatttgcga cagaaagcga
     3421 agtaaaagca gataaatttg aagaaaccga gagccctgcg cttattacga tcctcaaaac
     3481 cgcgcttaat ctcgagtagg agctttcact ttaagagcct catcaaaatt atcatcggca
     3541 gccattttat ttcgcttttt gacctggcat ttttgacatt caaatgtcaa cacgaatttg
     3601 cctttttcac tcgagagttc aactggcttc atgaaacctc cgcaaatagc ttctctgtct
     3661 ccggggttta cgtcaacgtg tttactgtag agacatgcgg gacagtgatt tgtataccca
     3721 gtccccttca cggtggtgcc gcagttatcg cagttaaaat cttctatctt ttttgtaaat
     3781 ttttttacca tacagaaaat tattgcacgc ctatcaggaa actccgaatc catttctacc
     3841 gcaacccttc tataatttta aaaacttaca agctcaaata tatatttcgg ggcgccgaga
     3901 tttgaactcg aactgcttga tcccaaatca agtatgctac cgttacacca cgccccgttc
     3961 ttttattatg actctgacaa ttctcattta ggaaacaggt caaatattag catagttcat
     4021 ccatttatgt actaaaagtc attaaaggac aatgtcttca agatactcga ctttcgcttc
     4081 cttcctatcc aaatctagat aaactgcgac tacatcgaat tgccaaacat cctcggctat
     4141 agccttttca gcgatgtaag tctgaatagt ccgtgacaat cgttccaatt tccaaggatg
     4201 catattatct tctggtctgt agcttgtctt ctgagcagac atgtccctaa gatttgtgcg
     4261 tgagacggtt ttcacttcaa taaaatgaaa ctttttgaac ttggcaatta tgtctatttc
     4321 accccatttt cttaaataat ttcgctctat tatcgaaaaa cccctactaa tcaaatactt
     4381 acaggcaata tcttcccctt tattacctat aatacgtttt tccgttaact gtttcatgtg
     4441 tcacattact aaattcttct gtatcagttc agcgacaaac acatcgagat ctttcatttg
     4501 ttccatctcg ctaatactag cccatttaca ctccgaactt tcgtcattca agactggaac
     4561 cccttcaatc tctccttgat atgtaagccg taccacatgt ttgtccggtc ttaaaatatc
     4621 ttgagcagct attaaaagtg gttctccctg gtattctagg cctgtttcct ccttaatttc
     4681 ccgacgcagg ttattaatca agtcaacccc aatattaatc ctaccaccca caatatccca
     4741 aacaccatcc gttttcggat atttttctgc agaacgtctt aacaatagaa attgacctgt
     4801 cttgtttttt agcaaaattt ttaccccaac ctgaagtttt atatgagcta gtttcatgtg
     4861 aatcatgttg cacgtgaaac taatgtttca agtgtaacaa tttaacgact ttagagctat
     4921 gctgagccgt atttcagaca ctttatgaga caacaccgta tgtcctttat caacatatac
     4981 acagacttat ccacaccttt actcttaaat acagccattt tggggaaaaa atgtgcatca
     5041 aaattgccca ataatcatgt cctttatctg tgcgggtacg gagaatcgaa ctccgatctc
     5101 gtccttggca aggacgcatt ctaccactaa accatacccg cgaacgctaa cctttatcga
     5161 aaaacagtta gaattataca ctgaagatcc attttttcta tgtctaaata acacgaaaaa
     5221 atatgttttc cggtcactgg cgctcccggc aggattctcg gctacgtcgc gtgattgcct
     5281 gtctcgccga ctcgacatac gcaatctttc tcatcctgac aaaagcaaat aaatttgctt
     5341 tttccggtca ctggcgctcc cggcaggatt cgaacccaca ataacggttc cgaagaccgt
     5401 tgtgatatcc atttcaccac gggagcgtgc ccgtataata cgtcatatta gtcaaaaaat
     5461 aaaaggtgta ctaaaattgc cccatgacta caaaacctcc tttattattt ctgaatatac
     5521 cgcaggaggt tgtgaatatt gtgcaaatga ttgcagaaaa ggggtttgag gcatatctca
     5581 ttggggggtg tacccgtgac ttgattattg gacggaaacc aaaggattgg gatatcacca
     5641 ccaatgcccg acccgatcaa attatagaga ttttcccaaa gaccttttac gaaaatactt
     5701 atggaacagt cggtgtcgta aatgaaaacg gggaagaccc gagcacaaaa attgttgagg
     5761 tcacccctta tcgacttgaa gcgtcgtatt ctgataaaag acgtccggac tcgatagagt
     5821 tcagtgaaaa actggaagat gacttaaaaa gacgcgattt cacaattaac gcgatcgcta
     5881 ttcaattgag gacgaaaaag aatgatgcat gggcatgtga agttgtcgat ctctttggag
     5941 gaatcaagga tttaaaaaat ggattggtta aaacagtagg ggatccacgt gagcgttttt
     6001 ttgaagatgc tttgcgtgtg cttagagcga ttcgtattgc tgtcgaacta gattttgaaa
     6061 ttgaaacaga gacagaaaag gcaattgcgg agtatgcgac acatttgaca catgtttcga
     6121 cagaacgact tcgtgatgaa tttacgcgaa ttttgatgtc ggacaaaccg tcgaaaggca
     6181 taaaaatggc cagaaatatg ggtattttga agtttattgc gcctgaatta gaagcgggta
     6241 ttggcatcaa acagaatcaa gcccacctgt acgatgtctg ggaacatctt ttacgtactg
     6301 tagaccatgc cgctaaaaaa acatggtcgc tcgatatacg acttgccgca cttttgcacg
     6361 atatatcaaa gcctgcaacg agagtatgga gtgacgagaa aaaagactgg accttttacg
     6421 gtcacgaggt tgtcggagca aaagttgcca aagatttgct aacacggctc aaatatccaa
     6481 aagagacaat tgataaagta ataaaacttg ttaggtggca catgtttttt tctgacaccg
     6541 aacaaataag tctctctgcc gtaagaagga tgattagaaa tgttggaccg gagaatgtgt
     6601 gggatctaat gaatgtacga atttgcgacc gcgtagggat cggaagacca aaagaagatc
     6661 cgtaccggct ccgtaagtac aaatcaatgg ttgaagaggc gctacgggac cctataagtg
     6721 taggaatgct taagattgat ggaaaaaaga tcatggaact gacacaggag ccgccgggac
     6781 ccaaggtagg ccatatactg catgcacttc ttgaagaagt actcgaggat ccaacaaaaa
     6841 atagtgttga atatttaaat agcagagtgc aagaattatc gaagcttgaa acatcggaac
     6901 taaagaggct aggggagtcc ggaaaggaaa ggaaagacac agaagatgag aaaaaattgg
     6961 atgaaatacg taaaaaatat tgggtgcgat aagtattata taaaataccc ataaacaaca
     7021 aagccgccat aggtgcggtc ttagagaaat accgaccggc ggctttataa agtgagacac
     7081 gcgcttcgcc gagaagtgag gacaagaaat gaaaatctaa gtggatgtgg aaattattgg
     7141 agatttattt cttgctctta aatcaagagt gagtaacgtg tggaaagaac agtcagattg
     7201 aattcttggc ttgtgtcaat ctgtgtccgt tatagattat acgttataaa ggacaacaag
     7261 aaaacatgcg gggtgtggat aaaagacatc cataaaacgg ctacaaagct tgctatttta
     7321 attcaaggag tattggacag tggtcagagc cgtatacgtt cgatagaatg tcgatttttt
     7381 ttacccgagc aagcaaattg ggcgtgataa aaaaatagtc tatacgccac ccaacatttc
     7441 tctctcgagc aaatgtatag gtgtcccaat aggtatacac aactttgccc ggattaaagt
     7501 agcgaaatac atctacataa ccgtgattta aaacctcgtc gagccattca cgctctaccg
     7561 gcaaaaaacc tgtacgagtt tcattttctt ttgggcgagc aagatcaatc tcctcatggg
     7621 ctgtatttac atcaccgcag attataagct tgggattctg ctttctgata agctcggcgt
     7681 acgtgagaaa cgcttcataa aaatctaatt tgtatttaag gcgctctggc ccaccgcctc
     7741 cattcggaaa ataaacgttt aatagcacaa aatcgctata aaaaacggca atcaaacgac
     7801 cttctttgtc cagggtttca actcccattt catatacaat tttttcaggc ttaatttttg
     7861 aatatgtggc cacaccactg tatccctttc ggcccttgga atgatcaaag tatgagtgat
     7921 agccaggagg attaacgatg gcgggcagaa gttgctcttt ttcactctta gtttcctgta
     7981 aacagatgat ttcagcgccc gtgttaacta accagtcaaa ttggcccttt tttgagagtg
     8041 cacgaaggcc gttaacgttc cagcatagta tctgcataag aaaatcctag cacattttgt
     8101 aagtatcgac gcggttcggg tcagatcact tccttttttt gccgtgaaaa ttcttatgga
     8161 tttcacgtaa atgacggtca gtcatgtgag tatatatttg tgtagtacca atatttgcgt
     8221 gaccgagcat catctgaacg gaacgtaaat ccgcaccgtt ttgcaacaag tcggtcgcaa
     8281 aactgtgacg gataacatgg ggtgtaactt tttttgatat accggccttg atggcgtaat
     8341 gtttcacaat acgctcaacc gagcgggcag tgagccgcaa tgacacaact tttgataagt
     8401 ctttttttat cgggccttgt acttgcacaa acaaagcatc gtccatatcc cttcgtgctt
     8461 cgagataagc ctttacggcc gctttggcgc gttcagacac gaaaacaacc cgaacttttt
     8521 cacccttgcc tcggatagaa aactcgtcag atttgaggtt gatttctctc gagagagagc
     8581 agagttcgga gacacgcagt cccgttgaaa acaagagttc caagatagct ttatcccgta
     8641 aacttcgtac atcgctccca gatgcggctt taagtatttt atcgagcgcc tcaagatcaa
     8701 ggagatcaag tgaccgctcg gctacttttg caagttcgat tcgctcgggg gcaagggcgc
     8761 ttatatttct tttggcgaga tatttcaaga acgcgcgcag tgcaattaaa tgaaaatttt
     8821 gcgtcttttt tttcaaggtc tcttcttcac catgtagttt ttgtcccgac ggttgccggt
     8881 tcagccataa tctaaagtca cggatggcgt cgtcagttat gtttttcacc ttgtctattc
     8941 tcgtaaaacc gaaaaaacgg ttgaggtaac gctcataatt ttctaccgtc ttaagacttc
     9001 tgcctttttc tatctcaaga tactctaaaa agttgcgcag cagatttttt acttcgcttt
     9061 tttcctccat tcttgttaag tatacccgat gtcgcacaat ctcgaccaaa aaagaacgtg
     9121 tcaaaggact tgcatagatt ggagatttat gctaccattt ccgccaatgc ttacaaagcg
     9181 aaagaaagag agggtaataa aagagaccca ggtccatgac aaggacacgg gatctcctga
     9241 agttcaggtg gcaattctca ccaaacgtat tgatgagctt gcaaaacatt taaaaactca
     9301 caccacggat aagcattcgc ggcgaggttt gctgcaaatg gtagcgaacc ggcagacgca
     9361 tcttggatat ttacaaaaga aaaatgttcg aagatacaac gccttggtga aaaaactcga
     9421 acttaagaag aaataatttc tacgcacgag cgtctctttt tttgtaagta gtacttttat
     9481 aagctagtat ttttttatgg ctgttatcaa acacaaagaa cagcgcgttg gtatttttat
     9541 agatacacaa aatctctacc atagcgctaa aaacctttat cacgccaagg taaatttcgg
     9601 acaaatcgtc aaagacgcgc tcgctggtcg cgcactcatc agggccgtcg cctacgttat
     9661 ctccacagaa agcggagagg aacggggatt ttttgaagcg ctcggaaaag tgggcatcga
     9721 gactaaagtc aaagacctgc aaattttcgg cagtggcgca aaaaaagcag actgggacgt
     9781 tggactggca gttgacgcca tcaaaatggc tccaaaactc gacactgtga tcattctttc
     9841 cggagacgga gactttatac cgctcgttga gtatcttcag atgaacgaag gttgccaagt
     9901 tgaagtagta tcattcggga aatcctcatc ccaaaaatta aaggaggcgt gcgatgaatt
     9961 tctcgatctc gatcaaaatc cccgcaaata tcttcttgga caatacaatc gcactcgtcg
    10021 tcctcggata caggcttcca atcatacaaa tagcgactcc tcaaacattc caacggtcac
    10081 cg
//
LOCUS       DATDSU010000160         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2494918, whole genome shotgun sequence.
ACCESSION   DATDSU010000160 DATDSU010000000
VERSION     DATDSU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494918"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VI981_05515"
     CDS             complement(<1..195)
                     /locus_tag="VI981_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479777.1"
                     /translation="MAGGNGIIESEPAKDRKSLRLFELQNSLTSSFLIHLKQPQSSAV
                     LAKVSFHKTISSAQHIGQQGV"
     gene            410..496
                     /locus_tag="VI981_05520"
     tRNA            410..496
                     /locus_tag="VI981_05520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Leu,seq:gag)
     gene            655..1041
                     /locus_tag="VI981_05525"
     CDS             655..1041
                     /locus_tag="VI981_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479778.1"
                     /translation="MPEEKFERKPIIAEMFDSLNPDADTDAMLERANAILTEAGQRSE
                     YDGSIATAYASEAFELLNHPNYEQKENAPLGIRNVKVWNNVTSGVTLRQYCQYLLERS
                     RENYVNEASGDHPKIATAVRDFIDKK"
     gene            1046..1534
                     /locus_tag="VI981_05530"
     CDS             1046..1534
                     /locus_tag="VI981_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4479779.1"
                     /translation="MAQRVFTQVFGVVAALIERDSKFLLVKEAKAGPDTGKWNHPAGW
                     IDVGENPVDAVKREVEEETGFTFTPKSILGIYSLVRKDIAKEMPGTPHALKIIFLGDI
                     SQKAEKELHDDVSETKWFTPEEIYAMEKHTLRDMEIKQMAKDYMAGISYPLALIYHHV
                     QE"
     gene            1594..1953
                     /locus_tag="VI981_05535"
     CDS             1594..1953
                     /locus_tag="VI981_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479780.1"
                     /translation="MESEKQALLEAVPSKVEGVYVPVGVPAELPRHKGRIQAYLGLAC
                     GLISIILFPPVFGIAGIILGIAAINRGEKVTGAIAVVISSVFMIAGFALGMYVSKHPD
                     IFKSGSYSTGAVYEALL"
BASE COUNT          581 a          409 c          522 g          575 t
ORIGIN      
        1 tactccttgt tggcctatgt gttgggctga cgaaattgtt ttatgaaaac tgacttttgc
       61 gagaactgcg gacgactgcg gttgtttgag atggattaaa aaagatgagg tgagagagtt
      121 ttgtaattca aagagtctta aagattttct gtctttcgcg ggctcactct ctattatacc
      181 gttcccgcct gccatcgctc gacacaccga atagcggcgt gatgttaaat agaaatttaa
      241 ccatcagtag tgcgaagcta gagagtgttt cgctctggct gtggcgagca ggcgcgcgaa
      301 taggtaaatg aagcaaaacg cgagagtttt cgagtctgtc agttttagaa aatttccgac
      361 agtaccttca gttgaaaaag acatttcctt tgggcataat gacccatacg cgcgggtggc
      421 gaaactagaa gacgcaatag cttgaggggc tatcggccgc aaggctgtgc aggtgcaagt
      481 cctgtcccgc gcaccatcga agaacttgag gaggtttcga ggaggagaga ccacggtttt
      541 aggcaggaac cacgggccac tttgcggtcg aggacaaggt tcgagccgat tgattcatac
      601 gaaaacacta aacaactttt gtgtatactt aagtcataat taatcgaaaa ctttatgcca
      661 gaagaaaaat ttgaacgtaa gcctattatt gccgaaatgt ttgatagttt aaatcctgac
      721 gcagatactg acgcaatgct cgagcgagca aacgccattt tgacggaagc aggacaacga
      781 agcgagtatg atggatctat tgccactgcc tacgcatctg aagcttttga attgctaaat
      841 catcctaact acgaacagaa agagaatgca cctcttggga ttcgtaatgt aaaagtttgg
      901 aacaatgtga cgtctggtgt gaccttgcgc caatattgtc agtatctgct tgaacgctct
      961 cgcgaaaatt atgtgaacga agcgtctggc gatcatccaa agatagctac tgctgtccga
     1021 gattttatag acaaaaagta aggcaatggc acaacgagtt tttacacaag tatttggagt
     1081 cgtggccgcg ctcattgaaa gagacagtaa attcctattg gtgaaagaag caaaggccgg
     1141 accggacact ggaaaatgga atcatccggc tggctggatt gatgtcgggg agaaccccgt
     1201 tgacgctgtt aagcgggaag tcgaagaaga aacagggttc acatttacgc caaaaagtat
     1261 tttggggatt tattcgcttg tacgcaaaga tattgcaaaa gaaatgcctg gtacgcctca
     1321 tgctttgaaa ataatttttc ttggtgatat ttcgcaaaaa gccgaaaagg aattgcatga
     1381 cgatgtctcg gagacaaaat ggtttactcc tgaagaaatt tacgccatgg agaaacacac
     1441 ccttcgtgac atggaaataa aacaaatggc aaaagactat atggctggaa tatcctatcc
     1501 gctcgcatta atttatcatc acgttcagga gtaattttca atggggataa gaaaattgat
     1561 ctcggtgtcc ttgtgtgtaa agattacgtc atcatggaat ctgaaaagca agctctactc
     1621 gaagcggtcc cgtccaaggt tgagggtgtg tatgtgcctg tcggtgttcc cgctgagctg
     1681 ccccgacaca agggacggat ccaagcttac ttgggtcttg cctgcggact catatcgatt
     1741 attttgtttc caccagtttt tggtattgcc ggtatcattt taggtattgc cgcaatcaac
     1801 agaggtgaaa aagtgacggg ggcgattgcc gtagttattt cttcagtctt tatgattgct
     1861 gggtttgcac ttggcatgta tgtgagcaaa catccagata tatttaaatc gggttcctat
     1921 agtacgggcg cagtgtacga agctctcctt tagatgatag tttatttcct ttttttcctg
     1981 acaatagtat tacctgtggt tctttgggtg ttgtttttgc gcggttccga cctctctgaa
     2041 ccagagccgg ggaagctcat gagaaagtgc ttttacagcg gtttttt
//
LOCUS       DATDSU010000161         4228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1824138, whole genome shotgun sequence.
ACCESSION   DATDSU010000161 DATDSU010000000
VERSION     DATDSU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824138"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VI981_05540"
     CDS             <1..848
                     /locus_tag="VI981_05540"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479781.1"
                     /translation="RRIADLVKIKIALQFYLQDNGFLPGRIDAQNIPVSPQYTESSTR
                     NPTDQTATGIAKCGSGLPTREWSPLGGNGNLQKLIPNYLPVLPKDPLNTPEQNGYCYI
                     YKPNPDLKGACVWALLERSSNNKVGIAVGRQDATLMGDGGKPVGFECNFLDEVIGGWD
                     AALGSGGGGSPPAPPSSYILNVAVSGNGNVAGTEPSFGINCGADCAETYGSETTVTII
                     ATPEGGESFIRWTGDCSGSDLSCTVLVNEIKSVTAEFTSVGGGTGSGGSGGSGGTGAS
                     GGSGS"
     gene            complement(845..3214)
                     /locus_tag="VI981_05545"
     CDS             complement(845..3214)
                     /locus_tag="VI981_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479782.1"
                     /translation="MPKKIATLFFKEWFIAGTLALIFFFINFHYFTTFITAWIFPGYD
                     GSSHVAIGEYYANHIFPKTWGWVPNWFAGLPFPQFYTPLFYFVAAIIYKILPFSFLTV
                     VKTIVLSNLFLVPYLLSRITRKLSGSTKAAWLTGLCAIFVLSTFYYSYSSTGITLQNS
                     INVGLVTQLFGFVLFLIWLEFFLEWRKSARAKFGAVIMLSLVLISNTHIVPIAMSLLV
                     VFFVGEFFSIIRKHQSGRSRIMGGLVKDYSILALLPLVLVSFWYIPLFYYWHFLAGIS
                     LDQVSGRIVFLTRYWPQLAGIIFGGMYAYRIRNKKMFFLAVAFFVIACIVSIPLYRIS
                     DGSLPIHTYRYIGPLGLLSLILLGTIMSALTNRFKRHIQILCFGLVILVFLVPFISSL
                     NAPRLAEFYLDEDKDEIKAMMRFFKERPELVSIENPGGVRNALTRGLYAELGREGGFG
                     NFSVFIEPSISSIFMPTVQNSFSRSTYAHAIHSSLYLSSDYLRRPLDERIDKAIMMGV
                     KFLVVQSPYIYDSFADSNRLREIANFGIWKVFEITDDVPLSFLPPQEPTLVFSSLSFK
                     KRTDDPYEYTRLQEELLWQNITKTVLAYANNQNLDETRDLDNFSSVLIANYTYKNKKN
                     ATERLTEYAKNNTLILLSSADPLFIELAAISNPHIHIIERGVGAYYSNATSATLEEIV
                     KTVEKLQKKMPVPQEKVHAERAGSCIKVSGGNGPPLVLKSSFFPSMRSSDNSPLYLAT
                     PTFTLGFFKENATVCMKTDPIIPFSYAISLVGLILAGFFYQKERKEKNN"
     gene            3311..>4228
                     /locus_tag="VI981_05550"
     CDS             3311..>4228
                     /locus_tag="VI981_05550"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEY4479783.1"
                     /translation="MNLDIKLLIVNIGHLLSIFLILALAVLVYVRGRKTSAAVPFLFL
                     SLVLSVHEAAYLLGINIENPAYSASAFYFTVVYIFIPCLSLHWIYAVLEIVNRKKIVL
                     SAFYILATVVASFYIVFPYSYVGPSLQKLYYTNYFNPGPYFFLSLIFFAGTALFGIFV
                     LAKHYAKVPVTQKKRLKYFLVAIIFAYTVGSTQFFLGFDILIDPFWSMFIGLYSVPLA
                     YAILRYDLMEISLVAKRAFVYALLVGVVSGLIILVNSVNNRIIDEVPGFPGWFIPIVS
                     GLVFILVASAVWRQIRSVDLLKYEFITVVT"
BASE COUNT         1197 a          867 c          985 g         1179 t
ORIGIN      
        1 cccgccgtat agccgacctc gtcaaaataa aaatagcact gcagttttac ctccaggata
       61 atggattttt accagggaga attgacgcac aaaacatacc ggtgagtccg cagtacacag
      121 aatcaagcac gcgcaatcca actgatcaaa cggctacagg catcgcaaag tgcgggtccg
      181 gtctgccgac tcgtgagtgg agtccgcttg gcggaaacgg caatcttcag aaacttatcc
      241 caaattattt acctgtgctt ccaaaagatc cgctcaatac ccctgaacag aatggctatt
      301 gttatattta taaaccgaat cctgacctga aaggcgcctg cgtttgggcg ctgcttgagc
      361 gttcatctaa taataaagta ggtatagccg tagggcgcca agacgctacc cttatgggag
      421 atggaggaaa gccagtgggt tttgagtgta actttttaga tgaggtaatt ggtggctggg
      481 atgctgcgct cggaagcgga ggaggaggat cgcctccagc gccgccgtcc tcgtatatat
      541 tgaatgtagc tgtatctgga aacggaaatg tggcaggtac agagccgagc ttcggcatta
      601 attgcggagc tgactgtgca gagacgtatg gcagcgaaac tactgtaacc ataatagcga
      661 ctcctgaagg gggcgagtcg ttcatacgct ggacgggtga ctgctcgggt agcgatctaa
      721 gctgcacagt acttgtcaat gagattaaat cagtcacggc tgaattcaca agtgtgggtg
      781 gtggaactgg tagcggtgga agtggcggaa gcggaggaac tggggcaagc ggtggaagcg
      841 gtagctagtt atttttctct ttccgttcct tttggtaaaa aaatccggcg aggattagcc
      901 ctacgagcga aatagcgtaa ctaaatggaa taatcggatc agtcttcata caaactgttg
      961 cattttcttt gaaaaatccg agggtgaatg ttggagtggc gaggtaaagg ggcgagttat
     1021 cgctcgatct catgcttggg aaaaaagatg atttaagaac tagaggaggg ccatttccgc
     1081 cagatacttt tatgcatgac cccgctcttt ctgcatggac tttttcttgt ggaacgggca
     1141 tctttttttg gagtttttcg acagttttca caatttcctc aagcgtggcg ctagttgcgt
     1201 tgctgtaata cgctcccacg cctcgttcaa ttatatgaat atggggattt gatatcgcag
     1261 cgagctctat aaacagcgga tcagcgctgg aaagtaaaat aagagtatta ttttttgcat
     1321 attccgttag acgctcggtt gcattttttt tgtttttgta tgtatagttc gcgatgagca
     1381 cactactaaa attatctaaa tcgcgagtct cgtctagatt ttgattgtta gcatacgcaa
     1441 gaactgtttt tgttatgttt tgccacaaaa gctcttcttg aaggcgcgtg tattcgtagg
     1501 gatcgtcggt cctttttttg aacgataacg atgaaaagac aagcgttggt tcctgcggag
     1561 gaagaaacga aagcggcacg tcatctgtta tttcaaaaac tttccaaatt ccgaaatttg
     1621 caatttctct aaggcggttg ctgtcggcaa aagagtcata aatatacggt gactgtacaa
     1681 ctaaaaattt tacgcccatc ataattgcct tgtcgatgcg ctcgtcgagt ggccgcctta
     1741 agtagtcgct actcaagtag agagacgaat gaatagcatg agcgtatgta gatcgggaga
     1801 aactgttttg aactgtcggc ataaatattg agcttatcga cggttctatg aatacggaga
     1861 aatttccaaa gccaccttcg cggccaagtt ctgcatacag tccgcgcgtc agagcgttgc
     1921 gaacaccccc cggattttcg atcgagacaa gttcaggcct ttctttaaag aaacgcatca
     1981 tcgctttgat ctcgtccttg tcctcgtcga gataaaattc agcaagtctg ggagcgttca
     2041 ggcttgaaat aaacggcact aaaaaaacaa gaatgacgag accgaagcat agaatctgaa
     2101 tatgccgttt gaatcggttt gtcaaagcgc tcattatagt tccgagcaaa atgagcgaaa
     2161 ggagaccaag cggtccgata tagcggtacg tatgtatggg taacgatccg tcgcttatgc
     2221 gatagagggg aattgatacg atgcacgcga taacgaaaaa agccactgct aagaaaaaca
     2281 tttttttatt tcggatacgg tatgcataca tgcccccaaa aattatccca gcaagttgag
     2341 gccagtacct cgtcaaaaag acgattcgtc cagacacctg atcgagactt ataccggcca
     2401 aaaaatgcca atagtaaaaa agtggaatat accaaaaaga gacgagcaca agcggtagga
     2461 gcgcaaggat ggaatagtcc ttaacgagcc cacccattat acgagagcga ccagattgat
     2521 gttttcgtat aattgaaaaa aactctccga caaaaaaaac aacgagaagt gacatggcga
     2581 tcgggacaat gtgagtgttt gatatgagca ctaaggaaag catgatcaca gctccgaatt
     2641 ttgccctggc gctttttctc cattccaaga aaaattccaa ccaaattaaa aaaagaacaa
     2701 acccaaagag ttgggtaacg agccccacgt tgatggagtt ttggagcgtg atgccggttg
     2761 aactatatga gtaataaaat gtcgacaaga caaagattgc acaaagaccc gtaagccagg
     2821 cggctttggt actgccgctt agttttctcg tgattcgtga caaaagatat ggtacgagaa
     2881 aaagatttga gagcacgatc gtctttacga cagttaggaa agaaaaagga aggattttgt
     2941 aaataatggc cgcaacaaaa taaaatagcg gagtataaaa ctgcggaaag ggtaggccgg
     3001 caaaccaatt cggaacccag ccccatgttt ttggaaaaat atggttggca taatattcac
     3061 caatcgccac atgagaactg ccgtcgtagc ccgggaaaat ccaggcggta atgaacgtcg
     3121 taaaatagtg aaaatttata aaaaagaaga taagcgcaag tgtgcctgcg atgaaccact
     3181 ccttgaaaaa tagtgtcgcg atttttttgg gcataaatat aagtggattg tctctcaaac
     3241 aatccactat ataatacagg ataagcgaat tttacttgtc taattattag tcagtctaca
     3301 ttctaatgga atgaaccttg atatcaagtt gcttattgta aatattggac accttctgtc
     3361 gattttcttg atcttggcac ttgctgtatt ggtatatgtc agaggaagaa aaacaagcgc
     3421 ggccgtccct tttttatttc tttcccttgt tctctctgtt catgaagcgg cgtatctttt
     3481 gggaataaat atagaaaacc cggcatattc tgcttcggca ttttatttta cggtcgtgta
     3541 tatattcatt ccgtgcctat cacttcattg gatttatgca gtacttgaaa ttgtcaaccg
     3601 aaagaaaata gttcttagcg ccttttatat tttggccact gtcgtggcgt ctttttatat
     3661 tgtttttccg tactcatatg tcggaccgtc gctacagaaa ctttattaca caaattattt
     3721 caatcccgga ccgtatttct tcttatccct tattttcttt gccggcaccg ccctctttgg
     3781 catatttgtc ctcgcaaaac actatgcaaa agtgccagtt acccagaaaa aaagactgaa
     3841 atattttcta gtcgccatta tttttgcata tacggttggc tcgactcagt tttttctcgg
     3901 atttgatatt ctcattgatc ccttttggtc catgtttatc ggactctata gtgtgccgct
     3961 cgcgtacgca atcttgagat acgaccttat ggaaatctcg ttggtcgcga agcgtgcctt
     4021 tgtctatgct cttcttgtcg gcgtggtttc cggactcatc atcctagtca acagtgtcaa
     4081 caatagaata atcgatgagg ttcccggttt tccgggatgg ttcatcccca ttgtttctgg
     4141 ccttgtcttt attctggttg cttccgcggt ttggcggcaa atacgatcag tcgatctttt
     4201 gaaatatgaa tttataacgg ttgttact
//
LOCUS       DATDSU010000162         8586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2498324, whole genome shotgun sequence.
ACCESSION   DATDSU010000162 DATDSU010000000
VERSION     DATDSU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498324"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..252)
                     /locus_tag="VI981_05555"
     CDS             complement(94..252)
                     /locus_tag="VI981_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479784.1"
                     /translation="MKKTQGGFSQIFGLIILLLVIGGAGTVYLGQKIYENRSKVLAKS
                     SLRSLAQG"
     gene            complement(473..1048)
                     /locus_tag="VI981_05560"
     CDS             complement(473..1048)
                     /locus_tag="VI981_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479785.1"
                     /translation="MNNLKKGFTWIIIAIILALIAGGGTYAAWRIQNSSKNEIQLPVS
                     EVGTNTSDQQESDNWKTYRNEKYGLEFGYPIDWVIREEMMATQSPTIVVRSPEHENYL
                     SNKPNAPYQGQYNDIVIVVHGYPWTDYNKDTAQSVIFNGMNALKFSYSGVYEAETYIV
                     TNNQTRTFTVTGLLDMESGTIKSILSSLKLH"
     gene            complement(1063..2718)
                     /locus_tag="VI981_05565"
     CDS             complement(1063..2718)
                     /locus_tag="VI981_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HEY4479786.1"
                     /translation="MNNLKKGFIVPVLLVIIALLVVGGSVYVYESKKSEAPAVINTET
                     QQSNQTQRQDNTPEPSIKNSITILSPNANTIWTTGETYQVKWTTSGIAMDSQGYILLK
                     NTAQGGDYYVINKSVALNGGAASNYGGSRNYTVPVESLSFLPGKNVIRIGVGRYEDYN
                     GAQSVYFDSAPFEIKGAVAKISVAGMKQYTDSNFGFSFWYPNTWTVQSTAIKNSYSGG
                     SIQKTLTIAPASDPNGTSGEAITIDEFSSPTREITIPRDICSPMSGLSVPIHRYYFDT
                     NTHTWMVEVPAYTGWSERDGSQYGVPASIKAADVSNNTMGGLHMLGAGCSGSVIPLSA
                     KNFVVFLFNSRNAGPNYIELAKTITATDPSVAMSVSSGEQIQTITKAGVLLGAIGTKV
                     GQWYVTNERVYNWRGDFIAGANPSTFRLIGTYSDGTTDASYATDGARVYSPWSAESPI
                     LSGADPVTFIAIRQQYQIPYAPSSGRYGESFTSYDTSFAKDKSHVWYLGKLIPGADPN
                     TFVVTGNTHVENTTGGYTLAHDTNYSYGIDKKGELTIDGVTVP"
     gene            complement(2758..3210)
                     /locus_tag="VI981_05570"
     CDS             complement(2758..3210)
                     /locus_tag="VI981_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479787.1"
                     /translation="MKKSLSVFVSVLVIASVVFIYSIKSLDKKTPDEIEKEVAAEIKD
                     NVTQARLVLDGKIRAVGERFLIAGFSVRLAAITPISFTIEHNDMLTSAERICQPLTGA
                     VEKTEVRQGTFLYQNTCDGGLAVEFTQFKISPDQKELMVTTKLYTSGL"
     gene            complement(3425..3496)
                     /locus_tag="VI981_05575"
     tRNA            complement(3425..3496)
                     /locus_tag="VI981_05575"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3462..3464),aa:Ile,seq:tat)
     gene            3601..4473
                     /locus_tag="VI981_05580"
     CDS             3601..4473
                     /locus_tag="VI981_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479788.1"
                     /translation="MNDDYIHNKIGPLAKFVAYLRTFSDIPYSLEIADMARAERAFLQ
                     QFGKNRELIEWAAPVVEARYKAIDYILILYGIRNILEVAAGLSPRGLAFTEDERTHYV
                     ETDLADIIQQKEILARKILQAKRTRGLNNLHFHPVNALVRGEIMTTLSFFDRSKKIAI
                     VHDGLLPYLTVAERKVFAANVHAILSFHGGIWVHTDVSLKNRYRNLVAGNSKLRNALF
                     TISTIINRDVEDNAFEDEADAEKFFKEAGFRITRWKLSKCVGGLASAAKLGVSDDKVG
                     ELLDTTYVWIMEPR"
     gene            complement(4555..6486)
                     /gene="ftsH"
                     /locus_tag="VI981_05585"
     CDS             complement(4555..6486)
                     /gene="ftsH"
                     /locus_tag="VI981_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY4479789.1"
                     /translation="MDFLNNNQKDNRKGKGPKNNMPLRNNFWNNLGIVFFVLIFIVGI
                     YSLIMEAKSRSPEIAISELARDINEKKISSIIVKGDELEVEYANGEQKKTKKETDTAL
                     TETLSNYGVSPEAMASVKIDVRGPSGFLFWLIQLAPFIVPILFIFIFIWFLTRQVRGA
                     GMQAFTFGQSKARMISPHDKKQKATFKDVAGAKEAKEELAEIVDFLKNPKKFIEIGAR
                     IPKGILLMGAPGTGKTLLARAVAGEAGVSFFSISGSEFVEMFVGVGASRVRDLFKLAK
                     QAAPAIIFIDEIDAVGRVRGTGVGGGNDEREQTLNQILVEMDGFEPNEKVIVMAATNR
                     PDVLDPALLRPGRFDRRVTIDLPDRKDRQEILTIHSKLKPFAEDVNLEVVAERTPGFS
                     GADLYSLMNEGAILAARENRKKVSQFDLIRSIEKVMLGPERRSHLLSKKEKEITAYHE
                     AGHALVASVLQYADPVHKISIIARGYAGGYTLKLPLEERKLQSKREFLDDIAMSLGGY
                     VTEQIVFGDLTTGPSNDLQVSTALARDMITKYGMSEKLGPLALEGTGGRTLYGSRGMN
                     EHEYSERVSAEIDGEVTRIMTEARTKAEKIVHEHKKLLDAIATRLIEVETIERDEFEK
                     LLIANGIVPKKKLDIEHQT"
     gene            6830..7276
                     /gene="smpB"
                     /locus_tag="VI981_05590"
     CDS             6830..7276
                     /gene="smpB"
                     /locus_tag="VI981_05590"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY4479790.1"
                     /translation="MSVLLTHKKIRLDYEILETFEVGIELSGPEVKALRQKQGELDGS
                     FVTVRGGEAFLLKTTIPPYQPNNITTSYDPIRNRKLLLTKKEIAKLGSIERQRGLTII
                     PSRFFEKGRKIKLEIAVVRGKKKYDKRETMKKEQAKRDVDRSLKGR"
     gene            7422..>8586
                     /locus_tag="VI981_05595"
     CDS             7422..>8586
                     /locus_tag="VI981_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY4479791.1"
                     /translation="MKAVRYITGFVFLITFVWVSSINAAHQNLTVSYTSEDVLTLATP
                     AVNRIVHVLRGNLELKLYTINLDTLQPVPDPKGSFRKVPVTETLTGAGFIVSQDGYIV
                     TNAHVVSPEEFRYGASLAAYTTALEQAVLKKMKAMSSREGANWLRDFDKRLKDMTLAQ
                     TLDERRAREQAFLKYVTFTDTGSETYAIKKDHSPGNLSALAKDGYRAKVITYDPNFVY
                     NGKDLAVIKINEANLPTLPLSDLDPLDQAEKIYTFGFPGSADFGAKNFAVPIFTPGTI
                     NSTKETVSGTYYYQVEAKVGVGSSGSPLLDTTGTVIGIITAESGSTGSTLATAIPSDA
                     IRTLLAQKNIAVHPSDYRDTYLDARQLESGQHCKRALETYDDAAALLNQGFVYG"
BASE COUNT         2357 a         2021 c         1790 g         2418 t
ORIGIN      
        1 tggtagtagc attccgagat attgtggtac cagtaccaga tgtctcgctc ttggtctgtc
       61 attcctcaat actacaccgt acggtgagcc aattcatccc tgcgccagag agcgcaggga
      121 gctcttggct aagactttag aacgattttc ataaattttt tgccctaaat agacagtacc
      181 agctccgcct atcacgagta aaagaataat aagaccaaaa atttgactaa agcctccttg
      241 agtttttttc atgttgacca aattgtattc ctcatccccc ctttccgcat ccccaacaca
      301 atcatctttc tttttaactc acttatgtac ttaacttact tatttcactt gtatacaata
      361 atatacgacc gtttattatt gtatacatgc ctgcctcaac aatacctcac ctcccgcgaa
      421 gctattcatt ttgagtagac tattttacct atcaataaag gtatgaccat tttcaatgta
      481 gtttaagcga tgagagaata gattttatgg ttccggattc catatcgagc agacccgtga
      541 ctgtaaatgt tcgggtttga ttgttggtta ctatgtacgt ttccgcttcg taaaccccac
      601 tgtaactaaa tttcaaggca ttcataccat tgaaaataac actttgagcg gtatctttat
      661 tgtagtctgt ccatggataa ccatgaacaa cgatgacaat atcgttatat tgaccctgat
      721 agggagcatt gggtttattg cttaaatagt tttcgtgttc tggagatcgg acaactatag
      781 tgggactctg tgtagccatc atctcctctc tgattaccca gtcgattgga taaccaaact
      841 ccaacccata cttttcattt cgatacgttt tccaattatc agattcctgc tgatctgaag
      901 tattggttcc aacttcagat actggcagtt gaatctcatt tttcgatgaa ttttgaattc
      961 tccacgccgc atacgttccc cctcctgcga tgagggcaag aattatggcg ataatgatcc
     1021 aagtaaaacc tttttttaag ttattcatat tgagagaata aattacggaa cagttacccc
     1081 atcgatggtt agttcgcctt ttttatcaat tccgtatgaa taattagtat catgagccag
     1141 tgtgtaacca ccagtagtat tttctacatg agtgttaccc gtaaccacaa aagtattcgg
     1201 gtccgctccg ggaattagtt tccctagata ccacacatgc gatttatctt tcgcaaaact
     1261 ggtatcgtaa ctcgtaaatg attcgccgta tcttcctgaa ctcggagcat aaggaatttg
     1321 atactgctgt cttatagcga taaacgttac tggatctgca ccagaaagaa tcggcgattc
     1381 agccgaccac ggggaataaa cacgcgcacc gtcagttgca tacgaagcgt ctgttgtgcc
     1441 atcagaatat gtaccaatga gtctgaatgt cgatggattt gcacccgcaa taaaatcgcc
     1501 tcgccagtta tatacccgct cgttggtgac ataccattga ccgactttgg taccaatagc
     1561 accgagtaaa actcctgcct tagtaatagt ttgtatttgc tcaccagagc ttactgacat
     1621 cgcgacagac ggatccgtag ctgtaattgt ctttgcaagc tcaatatagt taggtccggc
     1681 attcctacta ttgaacaaga aaacaacaaa atttttggca cttaggggta tgactgatcc
     1741 tgaacaacca gcaccaagca tatggagacc tcccatagta ttgttggaaa catccgcagc
     1801 ctttattgaa gcaggaacac catattgaga accgtctctt tcactccatc cagtataagc
     1861 aggaacttct accatccaag tatgcgtatt cgtgtcgaaa tagtaacgat gaatcggaac
     1921 ggataatcca ctcataggag agcaaatgtc tctaggtatt gtaatttcac gagtaggaga
     1981 agaaaattcg tcaattgtga tggcttcacc gctagttcca ttcggatcag atgcgggcgc
     2041 tatagtgagt gtcttctgaa ttgaaccgcc tgaataacta ttttttattg cagtgctttg
     2101 tactgtccaa gtgtttggat accaaaatga aaatccgaaa tttgagtcgg tatattgttt
     2161 cattccagct acagaaattt tagctactgc acccttaatc tcaaaaggtg cactatcaaa
     2221 atacacactt tgtgccccgt tataatcttc atagcggcct acgccaattc ttatcacatt
     2281 ttttcctggg agaaacgata acgattcaac aggaacagta taatttcggc tcccgccata
     2341 attagaagcg gctcccccgt tcaaagcgac tgatttattg ataacataat aatccccacc
     2401 ttgagcggta ttttttaaga ggatataacc ctgactgtcc atagctatcc ctgaagttgt
     2461 ccatttcact tgataggttt cgcctgttgt ccatatcgtg tttgcatttg gtgataagat
     2521 ggttatggag ttttttattg aaggttcggg cgtgttgtct tgccgttgag tttggtttga
     2581 ttgctgtgtt tcggtgttaa taacagctgg tgcttccgac tttttgcttt cataaacata
     2641 aacactaccg cccactacca atagcgcaat gataaccaac agcactggaa cgataaaacc
     2701 tttttttaag ttattcatgt cctcaatatt tttttctcat ttaatttgag gtgttgatca
     2761 caagccgctc gtatacaact ttgtcgtcac cataagctct ttttgatcgg gactgatttt
     2821 aaactgggta aactcgactg ccaacccgcc atcacaagta ttttgataca aaaaagtgcc
     2881 ttgccttacc tctgtctttt caactgcacc cgtaagcggc tggcaaatgc gttcggccga
     2941 cgtgagcata tcattgtgtt caatggtgaa gctgatcggc gtaatggcag caagtcgaac
     3001 ggaaaaaccg gcgatcaaaa aacgctctcc gaccgcgcgt atcttgccgt cgagaacgag
     3061 ccgcgcctgg gtcacattat ctttgatttc ggctgcaact tctttttcga tctcatcggg
     3121 agttttttta tcaagacttt taatgctgta tatgaaaaca acagacgcta ttacgagaac
     3181 tgatacaaaa acagaaaggc tctttttcat atgctttatt atatactgca agaaaaacgg
     3241 gtgcactagg gtaaattagt gaccaatttc atttttgtac acgcggagga acttgaaact
     3301 tacagtttca gtacaccttt cgaattttct cactttttga aaatagctcg aaaatatgct
     3361 caaggtctta aagtccctca cgcattgtgc gcaggcacaa tgctcgtgcc atcactttgt
     3421 tcggtacacg cggagggact tgaaccccca accaatgctg tataagagca tcgctctacc
     3481 attgagctac gcgtgtatgt catcagcata cgttacttat atatattttc aatgccattt
     3541 tctgccattg cagatgtgta tacatttgag aaagtgccac gtatactgaa tattaattac
     3601 atgaacgacg attacataca caataaaatc ggtcctctgg caaaatttgt ggcatattta
     3661 cgcactttca gtgatattcc ctattcactc gagatcgccg acatggcaag agcagagagg
     3721 gccttcctcc aacaatttgg aaaaaatcgc gagcttatag aatgggctgc gcccgtcgtg
     3781 gaggcccgct acaaagcgat cgactacatt ttaatactct acggcatccg caatattctt
     3841 gaagtcgcag caggcctctc tccgagaggc cttgctttta cagaagatga gagaactcac
     3901 tatgttgaga ctgatcttgc agatattatc cagcaaaaag aaattctggc cagaaaaatt
     3961 ttgcaggcga aaagaactcg cggactcaac aatcttcatt ttcatcctgt taatgcgctc
     4021 gttcgcggcg aaattatgac tacactctca tttttcgacc gaagcaaaaa aattgccatt
     4081 gtccacgacg gtctgttgcc ttatctcaca gtagccgagc ggaaagtatt tgccgcaaac
     4141 gtccatgcaa tcctgtcatt tcacggcggc atttgggttc atacggatgt gtcacttaaa
     4201 aacagatacc gcaatctcgt tgccggcaac agtaaactta gaaatgcgct gttcaccatt
     4261 tcgaccatca taaacagaga cgtggaagac aacgcgttcg aagacgaggc cgacgcagaa
     4321 aaattcttca aggaagctgg ttttcggatc actcgatgga agctctcaaa gtgcgtcggc
     4381 ggcttggcga gtgctgccaa actcggtgtt tcggatgaca aagtcggcga gcttctggac
     4441 actacgtacg tatggattat ggagcctagg tagaagggta tagggtttag aaaatttcta
     4501 aacgatttca tatctcgcct tctgccctac aaccctccac gctaaaccct tcttttatgt
     4561 ttgatgttct atatcgagtt ttttcttggg cacgattccg ttcgcgatca gtaatttctc
     4621 gaattcgtct cgttcaattg tttcaacctc aataagtcga gtggcaatgg cgtcgagaag
     4681 ttttttgtgt tcatggacaa ttttttcagc tttcgtgcgt gcctctgtca tgatgcgcgt
     4741 aacttcgccg tcaatttctg ccgaaacccg ttcagaatat tcgtgttcgt tcataccccg
     4801 cgacccatac agcgtccgtc cgcccgtgcc ttcaagcgcc aggggcccta gtttttctga
     4861 cattccatat ttcgtaatca tatcgcgtgc aagtgccgtc gatacttgaa gatcgttcga
     4921 ggggcctgtc gtgagatcac cgaaaacaat ttgttcagtt acgtacccgc cgagcgacat
     4981 ggcgatatcg tcgagaaatt cacgcttcga ctgaagcttc cgctcttcaa gcggcaattt
     5041 aagcgtatag cctccggcat atccgcgggc aatgatcgaa atcttgtgca ccggatcggc
     5101 atactgcaac acagacgcga cgagcgcgtg gcctgcctcg tggtacgcag tgatttcttt
     5161 ttctttcttc gataaaagat ggctcctccg ctcaggaccg agcataactt tttcaatgga
     5221 tcgaatgaga tcaaactgcg aaactttttt gcggttttct cgagccgcca aaatcgcacc
     5281 ttcattcatg agcgaataga ggtccgcgcc cgagaaaccg ggcgtccgtt cggcgacaac
     5341 ttctaggttc acatcttccg caaacggctt caacttagaa tgaattgtca aaatttcctg
     5401 gcgatctttt ctatccggca aatctatcgt gacgcgacgg tcaaaacggc cgggacgaag
     5461 gagcgcggga tcaagtacgt caggtcggtt cgttgccgcc ataacgatca ctttttcatt
     5521 tggttcgaaa ccatccattt caacaagaat ttgattgagt gtctgctccc tttcatcatt
     5581 gccgccgcca acaccggttc cgcggacgcg accaacagcg tcaatttcat caataaaaat
     5641 aattgcagga gccgcctgtt ttgcgagctt aaacaagtct ctgacacgcg acgcaccgac
     5701 acccacgaac atctccacaa attcagatcc ggaaatcgag aaaaacgaaa cgccagcctc
     5761 accggctacg gctctcgcaa gaagcgtctt tccggttccg ggtgcaccca tcagaagaat
     5821 tcctttgggt atacgggccc cgatttcaat aaattttttc ggatttttga gaaaatcgac
     5881 aatttcagct aattcttctt tggcttcctt ggcgcctgcc acgtctttaa acgtagcttt
     5941 ctgcttcttg tcatgcggcg aaatcatacg ggctttcgac tgcccgaaag taaacgcctg
     6001 catgccagcg cctcgtactt gccgcgtcaa aaaccaaata aaaatgaaaa taaaaagaat
     6061 cgggacaata aacggcgcca attgtatgag ccagaacaaa aatcctgacg gcccgcgaac
     6121 atcgatcttg acgctcgcca tggcttcagg cgaaacccca taatttgaaa gcgtttcagt
     6181 aagcgcggta tcagtttctt tttttgtctt tttttgctcg ccatttgcat actcgacttc
     6241 aagttcgtca cctttgacta taatcgaaga aatctttttc tcgttgatat ctctcgcaag
     6301 ctcggaaatg gcaatttccg gcgagcgaga cttcgcttcc ataataagcg aataaattcc
     6361 gacaataaat ataagcacaa aaaaaacaat tccgagattg ttccagaaat tattgcgcaa
     6421 gggcatattg tttttaggac ctttgccttt tcggttatct ttttggttat tgttgagaaa
     6481 atccatgggg tagtgaaaaa tgacatcact cttccggagt gatttttagc ttatttataa
     6541 ttttgattgt aatttttgat aaatcattga tgtcaaatta atgttggata gctctaatgc
     6601 cattttctac taccccatcc atccagtttg aagttagtta ataatctcga aatactttct
     6661 gaatccccac cgtgtttctg tgccgaaata cctgcattcc gaactgctgt tacctctgca
     6721 aatccgagac agagccaata catatcttca tgatttcgtt ggccgtcttg ttcattatgc
     6781 atacaaatat gagaattgta aaatcatggt ctcctgatat catgacagca tgtcagtctt
     6841 gcttacgcat aaaaaaatac ggctcgacta tgaaatcctc gagacattcg aagtcggtat
     6901 tgagctgtcc ggccccgaag taaaagcgct gcggcaaaaa caaggcgaac tcgatggttc
     6961 gtttgtgacc gtgcggggag gagaagcgtt tctgttaaaa actaccattc ccccttatca
     7021 accgaacaat attacgacaa gttatgaccc gatccgtaat cgaaaattat tactgacaaa
     7081 aaaggaaatc gcgaaacttg gcagcataga gcgccaaaga ggcctaacca tcattccgag
     7141 tcgtttcttt gaaaaaggcc ggaaaattaa acttgaaatc gctgtggtac gaggaaaaaa
     7201 gaaatacgac aaaagagaga ccatgaaaaa agaacaggct aagcgggacg tcgatcgctc
     7261 attgaaaggg cgctagattt ttcgcgcggg gcgaggcgac gtaggcattt tttgttgcaa
     7321 accgaccata atttctcaat tactgaatcg gtttttcaaa aaaagggtta ggaccgtaat
     7381 ccacagtaat aagcccgatc tgatgatatt atttaagagt tttgaaagca gttcgataca
     7441 tcactggctt tgttttttta attaccttcg tatgggtaag ttctatcaat gctgcccatc
     7501 aaaacctcac cgtaagctac acgagtgagg atgtcttgac gctcgccact ccggcggtca
     7561 atcgcattgt gcatgtgctt cgcggcaatc ttgaactcaa actctacacg atcaatctcg
     7621 atacgctcca gcctgtacct gatccgaagg ggtcctttcg caaggttccc gtaactgaaa
     7681 cgctcacggg ggccggtttt atagtgtcac aggatggata catcgttacc aatgcccacg
     7741 tcgtcagtcc ggaagagttc cgctatggcg catcgcttgc agcctatacg acagcgcttg
     7801 agcaagcagt actcaaaaaa atgaaagcaa tgtcgtcgcg cgagggtgca aactggcttc
     7861 gcgatttcga caagcggctg aaagatatga ctctcgcaca gacactcgat gagcgacgcg
     7921 ctagagaaca agcctttttg aaatacgtga ctttcacgga tacgggcagt gagacctatg
     7981 ccatcaagaa agaccattca cccgggaacc tgtccgcgct tgctaaagac ggatatcgcg
     8041 caaaggttat tacgtacgat ccgaacttcg tctacaacgg aaaagatctc gccgtcataa
     8101 aaataaatga ggccaacttg ccgacgttgc ctttgagtga tctcgaccca ctcgatcagg
     8161 cggagaaaat ttacacattt ggtttccctg gttcggctga ttttggcgcc aaaaattttg
     8221 ctgttccgat atttactccg ggaacaatca attcgacaaa ggaaactgta agcggaacat
     8281 attactatca agtcgaggcg aaagttggtg tggggtcaag cggcagtccc cttctggaca
     8341 ctacagggac tgtaatcggc atcattactg ccgagtctgg aagtaccggg agcacccttg
     8401 ccacagccat accgagcgac gctatcagaa ctcttctcgc tcaaaaaaac atagccgtac
     8461 atccgagtga ctatcgagat acctatttgg atgcgcggca actcgaatcc gggcagcact
     8521 gcaaacgagc gctcgaaaca tacgatgacg ctgcagcttt attgaatcag ggctttgtct
     8581 acggag
//
LOCUS       DATDSU010000163         4604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1830898, whole genome shotgun sequence.
ACCESSION   DATDSU010000163 DATDSU010000000
VERSION     DATDSU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830898"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VI981_05600"
     CDS             <1..421
                     /locus_tag="VI981_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY4479792.1"
                     /translation="HIEVIVRQMFSRRYVNKSGDTTLSEGDIASMGYLSAENKRAKDN
                     DGEEADTESVVMGITEISLSRRSFLSAASFQHTTRVLINAAIRGNEDKLVGLKENVII
                     GRLIPAGTGFEGSPKHTMIALKTRSQSEGAASEESNS"
     gene            474..2189
                     /gene="dnaG"
                     /locus_tag="VI981_05605"
     CDS             474..2189
                     /gene="dnaG"
                     /locus_tag="VI981_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY4479793.1"
                     /translation="MSSQVDLIKERLSIVDVVGSYLKLDRAGGNLKARCPFHNEKTPS
                     FFVSPARSSFYCFGCGAKGDIFTFVQEFEGLDFFGALKVLAERAGVELKRENREATDK
                     RNRLHDCLSEAEKYYSEQLLENTEALAYLRDRGLTEKDIFDWKLGFAPKEWRALYEHL
                     LRQHFTSVEILEAGLIKHKNDDPKNADYYDRMRSRITFPLFDSSGRVIGFSGRIFPQD
                     DTVAKYLNGPETSLFNKSSVLYGLHLAKHHFRERGFATVVEGQMDLILAHSRGYKNTV
                     AVSGTALTLMHLSTIKRFTSNVVFAFDPDRAGMNAALRSARLGLALGLDVKVARLPKG
                     QDPADLIRQDQASWERSLKAAEHVVLFALNAILDAKADEREKIKKIRDVVLPLVADIE
                     SSMEQGHFAEVVARRTSLPVDAVLQDVERIKKNVNETRETEAKVAPRVDSTERRIFGI
                     LMWQTTIDKPIIEIDKVGERLKLVAGQGRYDERLRYYKGVMSDVALEAEIFHNGSKDL
                     LADLEQLISSYEEDILKESFAEALKNLSEAERKKEGDKASEFLARCKEISERLSNLTK
                     NKPRV"
     gene            2246..3442
                     /locus_tag="VI981_05610"
     CDS             2246..3442
                     /locus_tag="VI981_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4479794.1"
                     /translation="MKKKKAHRAKKIKKARKLKSSKKQKAVPKAKKLKPVKLSKRARE
                     REDRAERLLAKGKERGFITYDEILKEFPTIEDDIVFLDELYAKLQQTGVDVLEGGGML
                     DLANEDLVGKKAAKSDASYDSIQMYLKEIGQYPLILAHQEKELAKRIEKGEDEAKNLL
                     ARANLRLVVSIAKKYVGRSPDLTLLDLIQEGNLGLFKAVDKFDWTKGYKFSTYATWWI
                     RQAITRALADQSRTIRVPVHMVETIAKYKQVVRRLSQDLGRDPLPEEIATEMGLDVDK
                     IYNIEKIDQDTVSLESPVGDDSDDGKSTLGDFLADDKILSPDHESSRRILGDQVKEIL
                     GDLSEKERQILEMRHGLGEWNGVTHTLEEVGQKFGVTRERIRQIEAKAHEKIRQHEKA
                     GRLKSY"
     gene            3602..3892
                     /locus_tag="VI981_05615"
     CDS             3602..3892
                     /locus_tag="VI981_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479795.1"
                     /translation="MKKGNKSATNIEILNSVEKLAISTATGFEDVQRQFRILDNRIGG
                     LEIGVAKANQKIDGLASRLDDFANTRVKIDIHATLVERVEHIETKLGIKRGQ"
     gene            4051..4407
                     /locus_tag="VI981_05620"
     CDS             4051..4407
                     /locus_tag="VI981_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479796.1"
                     /translation="MKAIFALLKLVVCIGTLFIFLASGISIAMGGGSTLLSVSIIGGI
                     IIVFSILMIIIVMLERKFDNKRVIVIAQNSIEKYKKQRGLTKTLIAVTIVSILAYLLY
                     GLYESIFPNIFFARPL"
BASE COUNT         1368 a          941 c         1131 g         1164 t
ORIGIN      
        1 gcacattgaa gtgatcgtcc gtcaaatgtt ttcaagacgg tatgtcaata agagcggcga
       61 taccactctt tcagaggggg atatagcaag catgggatat ttatcggcag aaaataaacg
      121 tgcaaaagac aatgatggcg aagaggccga cacagaatcg gtggtcatgg gtattactga
      181 aatttcactt tcaagacgaa gtttcctttc tgccgcgtca tttcagcaca caacgcgcgt
      241 cctcattaac gcggccattc gtggaaatga agataaacta gtcggactca aggaaaatgt
      301 catcatcgga aggcttattc ctgccggaac cgggtttgaa ggaagcccaa agcacaccat
      361 gatcgccctc aaaacacgaa gtcagtctga gggcgcggct tccgaagagt cgaattccta
      421 atagagtttg ttttggacgt aatatgggtt tgcgttagcc agacgctaag aaaatgtcat
      481 ctcaagttga tctcataaaa gaacgcctgt cgatcgtaga cgttgtggga tcgtacctga
      541 agcttgaccg tgcgggcgga aatttaaaag cacgctgtcc ttttcacaac gaaaagacgc
      601 catcattctt tgtttcgcct gcgcgttctt cgttctattg ttttgggtgc ggcgccaagg
      661 gtgacatttt tacatttgtg caggagtttg aaggtcttga ttttttcggg gccttaaagg
      721 ttctggcgga gcgcgccggc gtcgagctca agcgtgaaaa tagggaagcg accgataaac
      781 gtaatcgact acacgattgc ttaagtgaag cagagaagta ttattccgag cagttgcttg
      841 aaaatacgga ggctctcgcg tacctacgcg accgcggact tacggaaaaa gatatttttg
      901 actggaaact cggatttgcg ccaaaagagt ggcgcgcgct gtatgagcat ttgctgcgtc
      961 aacactttac gagcgttgag atccttgagg cgggactcat taagcacaaa aatgacgatc
     1021 cgaaaaatgc tgactactat gatcgcatga gatcgaggat cacattcccg ctttttgact
     1081 ccagcgggcg ggttatcggt ttttctggaa gaatttttcc ccaagacgac accgtcgcta
     1141 aatacctcaa tggtcctgaa acttcgttat ttaacaagag ttcggttctt tacggtcttc
     1201 atttggccaa gcatcacttt cgtgagcggg gctttgccac cgtcgtcgaa gggcaaatgg
     1261 atctcatcct ggcgcactca agaggatata aaaatacggt tgccgtgtcg ggaactgcgc
     1321 ttactctaat gcaccttagc accataaagc gttttacctc caatgttgta tttgcctttg
     1381 atccggaccg cgccggtatg aatgcggccc tgcggagtgc gcgacttggc cttgcgcttg
     1441 gactcgacgt caaggtggcg cgacttccta aaggccaaga ccctgccgac cttattcggc
     1501 aggaccaggc gtcttgggag cgttctctca aagcggctga acatgtggta ttatttgcgc
     1561 ttaatgccat attggacgct aaagctgacg agcgcgaaaa aataaaaaaa ataagggatg
     1621 ttgtactgcc cctagtggca gacattgaaa gtagcatgga gcaaggacat tttgctgaag
     1681 ttgttgcgcg acgcacatcg cttcctgtgg acgcagtgct tcaggatgta gagcgcatta
     1741 aaaaaaacgt taacgaaact cgggagacgg aagccaaagt ggcacctcgc gttgatagca
     1801 ccgaaagacg gattttcggt attcttatgt ggcaaacaac catcgacaag ccaattattg
     1861 aaatagataa agtcggggaa cgtctcaaac ttgtagctgg gcaagggcgt tacgatgaga
     1921 gacttcgcta ctacaagggt gttatgagtg atgtggctct cgaagcagag atatttcaca
     1981 atggctcaaa agatcttctc gctgatttgg aacagctaat ttcctcatac gaagaagata
     2041 ttttaaagga gtcattcgct gaggcgctta aaaatttatc ggaggccgaa agaaaaaagg
     2101 aaggagacaa agcatcggaa ttccttgctc ggtgcaagga aattagcgag cgtttatcaa
     2161 atttaacgaa gaataagccg agagtataag gaacattata agtgcgctgc atgagacggg
     2221 tgtgcagcga gtaaatggca aagagatgaa aaagaagaag gcgcaccgcg ccaaaaaaat
     2281 aaaaaaggca aggaagctaa agtcatctaa aaaacagaag gctgtcccta aggcaaaaaa
     2341 gttaaagccc gtaaagcttt caaagagagc ccgagagcga gaggacagag cagagcggct
     2401 tctcgccaaa ggaaaagagc gcggctttat tacatacgat gaaattctca aagagtttcc
     2461 gacaattgag gatgacatag tttttctcga tgaactgtat gcaaaattac agcaaactgg
     2521 cgtggacgtc ctcgagggtg gcggcatgct cgatctcgca aacgaagacc tcgttggaaa
     2581 aaaagcggcg aagagtgatg cctcatacga ttcgattcag atgtatttga aagagattgg
     2641 ccaatatccg ctcattttgg cccatcaaga aaaagagctg gcaaaacgca ttgaaaaagg
     2701 agaggatgag gcgaagaatt tgctcgctcg cgctaacctt agactcgtgg tgtcaatcgc
     2761 caagaagtac gtcggtagaa gtcctgatct tacactgctc gatcttattc aagagggtaa
     2821 tctcggtctt tttaaggctg tcgataaatt cgactggacc aagggataca agttttcaac
     2881 ctatgctact tggtggatta gacaggctat aacacgtgct cttgcggatc agtcgagaac
     2941 aattcgcgtt ccggtgcaca tggttgagac aatcgcaaaa tacaaacagg tggttcgtcg
     3001 tctttcacaa gatctcggac gggatcctct gcccgaggag atcgcaacag aaatggggct
     3061 agacgtcgat aaaatttata atattgaaaa aatcgatcaa gatacagttt cactcgagag
     3121 ccccgttggc gacgacagcg acgacggtaa atcaacattg ggagattttc tggcggacga
     3181 taaaattcta tcgcctgatc acgaatcatc acgcaggatt ttaggcgatc aggtaaagga
     3241 aatactcggt gacttatctg aaaaagagcg tcaaatactc gagatgcgcc acgggcttgg
     3301 cgagtggaac ggagtgacgc atacacttga agaagttggg cagaagtttg gtgtgacgcg
     3361 agagcgcatc cgtcagatcg aagccaaggc tcatgaaaaa attcgccagc acgaaaaggc
     3421 aggcagactg aagagctatt aaatgagaag cgtatagggt tgagcgtata gcgtatacca
     3481 ccttggagac ttagtctcca aggtggggat caccatccag aaagacttat gttaagccat
     3541 atatttccac aaggtcggac cttgaaatta tgagtcgcgg gtcacgtggt ataatatcgt
     3601 catgaaaaaa gggaataagt cggccacaaa tatcgaaata ttaaattcag tcgaaaaact
     3661 agcgatttca actgcaactg gttttgaaga tgtccagagg caatttcgaa ttttggacaa
     3721 tcgaatcggt ggcctagaaa taggtgttgc aaaggcaaat caaaagattg atgggttggc
     3781 gagtcggcta gacgattttg caaatacgcg agtcaagatt gatatccatg caactttagt
     3841 tgagagagtc gaacatattg agacaaagtt aggcataaaa cgtggccaat aattcaaagt
     3901 cgaacctttt tgcgatttat ggttcgacct tgaatcagac atctatctgc tactcactat
     3961 tcactaccca ctaatcacta ttcacaccca aaaaatggaa ccacaaacta aaaaaagata
     4021 ttggttgagg ggcttgataa tcaataatcc atgaaggcaa tctttgcatt acttaaatta
     4081 gtcgtctgta ttggaactct ttttattttt ttagcttcgg gtattagtat tgctatgggc
     4141 ggtggaagca cccttctttc tgtgtcgatc attggtggga taattatcgt cttttctatt
     4201 ctaatgataa taattgtaat gcttgaacgt aaatttgata ataaacgcgt catagttatt
     4261 gcacaaaata gtatagaaaa atataaaaaa caacgcggcc taaccaagac acttattgca
     4321 gtcactattg tatctatatt ggcatatttg ctatacggct tatatgaatc catatttcca
     4381 aacatctttt ttgctcggcc actgtaatca ccgttattcc ccctgtttct gctggtggtg
     4441 atcggtaagg gctatggcaa aatcaaacat cgtttgtctt ttttcttctg ttgtcatttc
     4501 tttgattaaa acaacttgta attttgttcc ctcgacagaa acaaatgtgc aagtcggctg
     4561 aagccccagt atgcgagagt catgtctcct taggagcgac tatc
//
LOCUS       DATDSU010000164        11584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2500816, whole genome shotgun sequence.
ACCESSION   DATDSU010000164 DATDSU010000000
VERSION     DATDSU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11584
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500816"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VI981_05625"
     CDS             <1..638
                     /locus_tag="VI981_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4479797.1"
                     /translation="PLTSIRWAAENLNRAVTAEEKGRDLALISEASLRLEELTDRLVG
                     LTDTNVKGKYNYEKTDLHELAVTLLAYYVAKSSEKHITIVDTIATDVTAVVDRERIKF
                     VFQIILDNAVTYTPPGGKIIVRLERNKENVLFTVKDSGIGFTKEERPYLFNKFFRTED
                     AKRLDTEGMGLGMYLAKEIIEIHGGTITATSEGRNMGALFVASVPLERATR"
     gene            complement(650..1681)
                     /locus_tag="VI981_05630"
     CDS             complement(650..1681)
                     /locus_tag="VI981_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4479798.1"
                     /translation="MRKAVKLLGTLAAIVIFVGGLYFARSRFGSELQDVTNTIFTFSA
                     AGDYAANLETDRVLEAIKVKNPDFHIALGDFSYNTLKPESKWCEYVRGHIGQSIPFEL
                     ISGNHESNGKDGLITNFVKCLPSKIENLTGTYGKEYYFDYPREKPLARIILISPNLDF
                     VDQAAYNYTARTPQLDWLLNAITSARSQNIPWVIVGMHKDCISPSRRTCEITPQVMNV
                     LINNKVDVILQAHSHIYARSKPLLCVSADTRGVIFEQACTTENNLHNIYNKGEGSLLL
                     ISGTGGQTLTDITPTSEAVNYFEKYAGKNYNPTFGFVYFKVNEQEIRGEFVSVNNALT
                     DSFVVKTSR"
     gene            1877..4744
                     /locus_tag="VI981_05635"
     CDS             1877..4744
                     /locus_tag="VI981_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4479799.1"
                     /translation="MILKGNFLWKGVLIVIGLLWAGQASALTTPAGFQVETVTSGLNL
                     PTTFAFAPDGRIFIAEKNGTVRLYKNGALQAAPVYVIPRINDYADHGLLGLALDPQFA
                     QNGYMYLSYTYENTPGVNYSGPKTGQIVRVTVEGDTANDSSKVVLVGQVTGDASNPSC
                     ENYAVTADCIPSDSQTHSMGGLRFGPDGKLYATIGDGAGFESADPKAMRALNVDSLAG
                     KLLRINRDGTAPADNPYYNGNANANRSKVWSYGLRNAFRFNWRPSNGALYLADVGWMR
                     FEELNNAQSGNNFGWPCREGYESVPEYNCATANYVNPMYAYDHSPGSGAIAGGAYPLN
                     NIYPQSYMGSFYVADYAQNWIKKFNIGENNVVNSVEEFITDAGGPVDILAGNDGTIYY
                     MSIYTGELRRIIYSNANRSPVVQLASDKNYAPVPMTTNFSSNGSYDPDGDPLSYAWTF
                     GDGGTSNEANPSHTYNSANTYTVSLAVSDGRGGSDTKSITVHPGQTPPATVLDPRHVR
                     TTVAPLPTYIGRDSVVTTTITNDGGGSDPFIVDIEIYDSNVVLAAKKFYDSQVVSAGT
                     SRDFSLTWFPPHIGEFVVKIGLFRNDWGQLYEWTDRALVVNVLERSTETPPPPPSNPP
                     PPPTNPPPPPPPTNLIPNPSLETGGARPDSWASGSWGSNSPSFTYPVAGFDGSRGAKV
                     TMTRYASGDAKWFVTPVTVSPDTEYKFSDYYKSTVPTDVVIEYTAANGVHSYIWLGTL
                     PTNANWTKFTKRFTTPANAARATFFHLISKNGELTTDLYSLTSIVDGTTPPPSNPPPP
                     PTNPPPPPAADVIYGDSLATGWVNWSWGSTIDLGATSPVGEGTRSIRIVYQSPWSALY
                     LHRSQFDTSGKTNIIFEVNGGSASGQIIKIVPYDGNGNPIEGTYLAPYIAGGITANTW
                     KTVTVPLADIGAANKVMTGFAIQDGRGLVEAPLYLDFVRFQ"
     gene            complement(4934..5959)
                     /gene="mltG"
                     /locus_tag="VI981_05640"
     CDS             complement(4934..5959)
                     /gene="mltG"
                     /locus_tag="VI981_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY4479800.1"
                     /translation="MAKRKTRRSKKGSSGRQINSVSIKKKIWSSVAIIAFAITAFLFY
                     PSTKDVPREPLEVVSSATASKTEILDELYEKGYIKSKLSYPVLLILSTFYKGIKRGVY
                     ALTPDMNLWQIYLQLANPQARWVRVEEGLRKEEVANVLAKQLGWKDTDKEKFVNAHLA
                     LKRDNLEGYYFPENYLIPLDADPLSISKKMIQNFDEKIARSRKSATKTIINGETALTI
                     ASIIQREAAGKSDMNLISGIIWNRLFAGMNLQIDATLQYAKGNEKNGWWPSVSPRDKK
                     IDSPYNTYQHDGLPPSPISNPGLAAIEAAYNPQKTACLFYFHDANRKIHCSKTYAEHK
                     ALVEKYY"
     gene            complement(6220..6624)
                     /locus_tag="VI981_05645"
     CDS             complement(6220..6624)
                     /locus_tag="VI981_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479801.1"
                     /translation="MNSKQFLVIGGIILVVVGILGWINVLGPTADSSIFGSGWWFDTA
                     ENWAHFVLGIVALVVAFLLPASTHKPIVIVVGIIAILAALYNLAGTKLLGANLESPAD
                     LILHLVIGLWALYAALNKRKATPTAPNTSMPM"
     gene            6987..7340
                     /locus_tag="VI981_05650"
     CDS             6987..7340
                     /locus_tag="VI981_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479802.1"
                     /translation="MSVPALPTGSAFEVSRTHVFCNPERKWVQPLRGVFLDHRIPDSI
                     DLSSVMETVHEVKLGIRGGVRRIEVFPYQWVWDDNFVSVAICCQISINPPCQLFLLFS
                     IRDRKLATFGGLEEY"
     gene            7396..7755
                     /locus_tag="VI981_05655"
     CDS             7396..7755
                     /locus_tag="VI981_05655"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY4479803.1"
                     /translation="MKLRELARKKCVPCKGGMKPIGSPEAKNYLAELPLWQLDKDEKK
                     ISREFTFDNFANAMIFVNCVAHIAEEEGHHPDIHIYYDKVKLSLTTHAIDGLSLNDFI
                     LAARIITDCTDKPEILQ"
     gene            7752..9170
                     /locus_tag="VI981_05660"
     CDS             7752..9170
                     /locus_tag="VI981_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479804.1"
                     /translation="MISTSKIKERDEPVTFIIFGATGDLALLKLIPALFRLFKKGYLS
                     SEFRIVGFARRPFTNQEYRALVASSMSEETKVTEDYQKFLQQIAYQQGQFDEEKTYRN
                     LMLLLDELDDKMGSCSHKIFHFAISPFYYETVLTNLNKTGLTISCTDGSGRTRVLLEK
                     PIGTDGESAEHIQALLSGIFREDQIFRIDHYLAKRGVSDFLMFRYENPAFETVLNADF
                     VEEVFIEQFETRTIEGRGGFYDHIGALRDVGQNHLLEILALIAMERPRKDSAEAFSLM
                     RAQVLSELIIPDSAAIERKVKRGQYKGYLEEEGVAPFSNTETYFRIPVFIKSHRWRRV
                     PFVITSGKALLESRATIRIIFRRTVEFKSLVDKETRKADMLVFSIQPESRVEFCERGL
                     KSTRDSYTCAISTYEEVGVPASTPVDEYERIYLDALAEDKSIFVSRSEVKAAWKYIDG
                     IVMAWREKALVIYKKGSNPDDL"
     gene            9189..9959
                     /locus_tag="VI981_05665"
     CDS             9189..9959
                     /locus_tag="VI981_05665"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4479805.1"
                     /translation="MGTISVKTYRRGLEKKAAQAVSILLHKYKTKPILLMVSGGSALK
                     ILNHTIADTFGSKITVTVLDERFSSDPAVNNFLQIAISEFYEQAKKNNVNFLITVPLP
                     NEHIQDIADRWQFDIRLWLANHPGGKVVALMGMGEDGHTAGIMPYPENEWIFNELFED
                     ENKWVVAYDALSKNQHPQRITTTTHFLKHVIDEAILFVTGEQKKEMLRRALAPDGKVH
                     EYPVRIIHEMKQVTVFTDIILQKVDNRKKTEVNQIKAN"
     gene            10027..10263
                     /locus_tag="VI981_05670"
     CDS             10027..10263
                     /locus_tag="VI981_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479806.1"
                     /translation="MGVKDWLISSGMKQMLNQQLKNVPEAERQKLVSLVEKNPEFFMK
                     IAGDIKAKVDAGTGEMTAAMEVMKENEAELCGMI"
     gene            complement(10304..10954)
                     /locus_tag="VI981_05675"
     CDS             complement(10304..10954)
                     /locus_tag="VI981_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY4479807.1"
                     /translation="MRKISFALGEYYHIYNRGVDKRNIFADFFDINRFFLSMNEFNTP
                     VSIGSIYEHRFDKPDNSVPKPKLIEFTAYCLNPNHFHFIVTPQHDAGIQKFMHKLGLG
                     YTNYFNERHKRSGSLFQGKYKAVHVNSNEYLLHLSAYVNLNDEVHQLGNSVPKLVKSS
                     WEEFLDPATKNPFCRKNIVLDQFATFPAYKKFALDCLDSIRLRKKMNMQLEKDLFD"
     gene            11058..11324
                     /locus_tag="VI981_05680"
     CDS             11058..11324
                     /locus_tag="VI981_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY4479808.1"
                     /translation="MSEIGVESLSSPGCHNCEAFERFWDGVKSHYPNVKYTNKSILTP
                     EGQEMAQKFGIFASPGIVINGELFSTGGVSTDKFIAKIKELSSR"
BASE COUNT         3272 a         2408 c         2673 g         3231 t
ORIGIN      
        1 ctccgcttac gtcaattcgc tgggcagccg aaaacttgaa tcgggccgtt acagcggaag
       61 aaaaaggccg cgacctcgca ctcattagtg aggccagtct acgactcgaa gaattgactg
      121 atcggcttgt cggtctcact gatacgaatg tcaaaggaaa atacaactat gaaaagacag
      181 atttgcatga actcgcggtc acactcctcg cgtattatgt cgctaagtca agcgagaaac
      241 atataactat tgttgatacg atcgcgacgg atgttaccgc agtagttgac agagaaagaa
      301 tcaaattcgt ctttcagata attctcgata atgcagttac ttatacacct ccgggaggga
      361 aaataattgt tcgactcgaa cgcaataaag aaaatgtact ttttacagtc aaagacagtg
      421 gtatcggatt taccaaggaa gagcggcctt atttgttcaa taaatttttt cgaaccgaag
      481 atgcaaaacg acttgatacg gaaggtatgg gactcggtat gtatcttgca aaagaaatta
      541 tagaaataca cggcggtacc atcaccgcta cgtcggaggg aaggaatatg ggtgcgcttt
      601 ttgttgccag cgtaccgctt gaacgggcca cacgttgatg caagacggct tatcgagatg
      661 ttttaacaac gaacgagtcg gttaaagcgt tatttacgga cacgaattca ccccgtattt
      721 cttgctcatt gaccttaaaa tagacaaagc cgaatgtcgg attgtaattt tttccggcat
      781 atttttcaaa atagttcacc gcctcgctgg tcggagttat gtcagtgagt gtctgtcctc
      841 ccgttcctga aataagcaga agtgagcctt cgcctttatt gtatatatta tgtaaattgt
      901 tttccgttgt gcacgcttgt tcgaaaataa cgccgcgggt gtctgcgctg acgcacagaa
      961 gcggtttgct ccgcgcataa atatgactgt gagcttgcag aataacatct actttgttat
     1021 tgatgagcac attcatgact tgaggcgtta tttcacatgt tcgtctcgaa ggacttatgc
     1081 agtccttgtg catgccgacg atgacccaag gaatgttttg agatcgggcg ctagtaatgg
     1141 cattaagaag ccaatcaagt tgaggagttc gagccgtata attatatgca gcctgatcaa
     1201 caaagtccaa attcggtgaa atcaaaatga tacgcgcgag cggcttctcg cggggataat
     1261 caaaataata ttccttgccg taagtgcctg tcaggttttc tatttttgac gggagacact
     1321 ttacgaaatt agttattaac ccgtccttgc cgttactttc atgattgcct gaaatgagtt
     1381 cgaaaggaat ggattgacca atgtgacctc tcacatactc gcaccacttt gactccggtt
     1441 taagggtgtt gtaactaaaa tccccaagtg cgatatgaaa atcaggattc ttaactttta
     1501 tggcttcaag tactcgatcg gtttcaagat tggcagcata gtctcccgcc gcggagaacg
     1561 taaatatggt attcgttacg tcttggagtt ctgatccgaa tcgactgcgg gcaaaataaa
     1621 gcccgccaac aaaaataaca atagcggcaa gtgttcccag taatttgacg gcttttcgca
     1681 tacgtgcatt ttaatacgaa agcttgactt tacgctgggg aaaggtaatt tagacattac
     1741 agacgatttg ctctctttga gatagacagg agacaagaaa cgatgcatga tgcttccggc
     1801 cgtttgcgtt ggcaacgtag cacgtttaaa aacgtcttta agttgcaaca gcctgtttaa
     1861 cattttttat aatattatga tactaaaagg gaatttttta tggaaagggg tacttatcgt
     1921 aattggactt ttgtgggctg ggcaagcatc ggctcttacg acccctgccg gatttcaggt
     1981 tgaaaccgtt acatcaggac tcaatttgcc caccacgttt gcgtttgcac cagacggacg
     2041 aatttttata gcagagaaaa acggtaccgt aagactctat aaaaacgggg cgcttcaggc
     2101 cgcgccggtg tacgtcattc cgcgaattaa tgattatgcc gaccacgggc ttctcggact
     2161 tgcgctcgat cctcaatttg cacaaaacgg ctatatgtac ctctcgtaca catacgagaa
     2221 tactccaggc gtaaattaca gcggtccgaa aacaggtcaa atcgttcggg tgactgtaga
     2281 gggtgacaca gcgaatgatt catccaaagt tgtacttgtc ggacaggtga ccggtgatgc
     2341 gtcgaatcca tcatgcgaaa actatgcggt tacagccgac tgtattccat ctgattcgca
     2401 gactcattca atgggaggac ttcgctttgg accggacgga aaattgtacg cgacaattgg
     2461 agatggcgcc gggtttgaat cagctgaccc aaaagccatg agagcgctta acgtagacag
     2521 tcttgccgga aaattacttc gcatcaatcg agacggtaca gcgcccgctg acaatccata
     2581 ttacaacggc aatgcgaacg ccaacagatc gaaagtctgg agttatgggc ttcgcaatgc
     2641 cttcagattc aattggagac cctcaaacgg agcgctctat cttgctgacg tcggttggat
     2701 gcgttttgaa gaattaaata acgcacaaag cggaaacaat tttggctggc catgcaggga
     2761 agggtatgag agcgttcccg aatacaactg tgcgacggca aattatgtga atccgatgta
     2821 tgcatatgat cactctccag gtagcggagc tatcgcgggc ggtgcgtatc ctttgaacaa
     2881 tatttatccg caaagctaca tgggaagctt ttatgttgct gattacgccc agaactggat
     2941 taaaaaattt aatattggcg agaataatgt cgtaaacagc gtcgaagaat ttatcactga
     3001 cgctggcggt ccagtagata ttttggccgg aaatgacggg acaatttatt atatgtcgat
     3061 ttacacggga gaacttcgaa gaattattta ttcgaacgca aacagatcgc cggttgtgca
     3121 acttgcttca gacaaaaatt acgcgccggt tccgatgacg acaaacttct catcaaatgg
     3181 ttcgtacgat ccggacggtg acccattatc gtacgcgtgg acatttggtg atggaggcac
     3241 ttcaaatgaa gcaaatccgt ctcatacgta caatagtgca aatacttaca ctgtgtcctt
     3301 ggcggtgtcg gacggaaggg gaggatcaga cacaaaaagc attactgttc acccggggca
     3361 aacgccgcct gcaacggtgc ttgacccacg tcacgtgaga acaaccgttg cgccacttcc
     3421 gacatatatt ggccgcgatt cagtggtaac gactactata acgaacgatg gcgggggaag
     3481 tgatccgttt attgttgata tagaaattta tgattcaaac gtggttttgg ctgcaaagaa
     3541 attttacgac agtcaggttg tctcggctgg aacgtctcgt gacttttctc taacgtggtt
     3601 tcctccgcat atcggagaat ttgtagttaa aattggactt tttagaaatg actggggcca
     3661 actttacgaa tggacagatc gcgcattagt tgtaaacgta cttgagcgat ctacggaaac
     3721 accgcctcct cctccgtcta acccgccgcc tccgccaact aatcctcctc cgcccccgcc
     3781 tccgacgaat ctcattccta atccgtcgct tgaaacagga ggtgcgcgtc ctgactcatg
     3841 ggccagtggc tcatggggat cgaactcacc ctcgtttaca taccctgttg ccggttttga
     3901 cggatcacgt ggcgcgaaag ttaccatgac tcgctatgca agcggcgacg cgaaatggtt
     3961 tgtcactcct gtgacagtga gccctgacac agagtataag ttcagcgact actacaagag
     4021 taccgttccg acagacgtgg ttattgaata caccgccgca aatggagttc actcatacat
     4081 ttggctcggt actttgccaa cgaacgcaaa ttggactaaa tttacgaaac gatttaccac
     4141 tccggctaat gccgctcgtg ccacgttttt tcacttgatt tcaaaaaatg gcgagctgac
     4201 gacggatctc tacagcttga ccagtattgt cgacggaaca acaccgcctc cgtctaaccc
     4261 gccgcctccg ccaactaatc ctcctccgcc tcctgccgcg gacgtaatat acggcgattc
     4321 actggctaca ggctgggtaa attggtcatg gggcagtacg attgacttag gagctacgtc
     4381 accagtcggc gaggggacgc gatctattcg aattgtatac caaagtccgt ggtccgcgct
     4441 ctatttacat cggagtcagt tcgatacgtc tggaaaaaca aatattattt ttgaagtcaa
     4501 tggaggctcg gcgagtggtc aaataattaa aatcgttccg tatgatggta acggcaaccc
     4561 gatcgaggga acctatcttg cgccgtatat cgctggtggt ataactgcga atacctggaa
     4621 aacagttacc gttccgcttg cagatatcgg ggcggcgaat aaagtgatga caggttttgc
     4681 tattcaggac ggcagaggac tcgttgaagc accgctttat ctcgatttcg tccgatttca
     4741 gtaaagggcg ctcgcccagc accatttttg atccagtaaa gattcataaa aacaaaaatg
     4801 gtgctgggct cgcttggttg caaaatttca aggttggacc ttgaataagc agtaactggg
     4861 ccgcgcttca ctcatcattt caaggtcgga ccttggaata tgtcgcgaat aatgagagac
     4921 gccatattct gcgttagtaa tatttctcaa caagagcttt gtgttcggcg tatgtctttg
     4981 agcagtggat ttttcggttt gcgtcgtgga aatagaataa acacgccgtt ttttggggat
     5041 tgtacgcggc ctcgatggca gcaagtccgg ggtttgaaat gggggaaggc ggcaacccat
     5101 catgctgata ggtgttgtag ggtgaatcta tttttttgtc tcgaggacta actgacggcc
     5161 accatccatt tttttcattt cccttggcgt attggagtgt cgcgtctatt tgaagattca
     5221 taccggcaaa caggcgattc caaattattc ctgaaatcag attcatatca cttttgccag
     5281 ccgcctcacg ttggatgatg gacgcaatcg tgagcgcagt ctctccattg atgatagttt
     5341 tcgtagcgct ctttctagat cgtgctattt tctcatcaaa attttgaatc atttttttac
     5401 taatggaaag aggatcggcg tcaagtggaa tcaaataatt ttccgggaaa taatatcctt
     5461 cgagattatc gcgcttgagc gctaagtgcg cgttgacgaa tttttccttg tcagtgtctt
     5521 tccagccgag ctgtttggcg aggacatttg cgacttcttc tttacgcaag ccttcttcga
     5581 ctctgaccca tcgagcttga ggattggcga gttgcaaata gatttgccac agattcatgt
     5641 cgggcgtaag cgcatagacg ccccgtttta tacctttgta aaaagtactc aatatcaata
     5701 atacggggta gctcaatttg ctttttatat agcctttttc atagagctcg tctaaaattt
     5761 ctgtctttga tgccgtcgct gaagacacga cttcaagcgg ttcacgcggg acgtcttttg
     5821 tagatgggta aaataaaaaa gcggttatag caaaggcgat tattgccaca ctgctccata
     5881 tctttttttt gattgataca gaattgatct gtctaccaga agatcctttt tttgaacgtc
     5941 tggtttttct ttttgccatg tgtcggtaaa ttgtataaat ttaaacgcta ttcgtcacgg
     6001 taaaacgtga gcggcgtctt tgtaaaaaaa gttttaggta ctattcgagc tctgcccata
     6061 ctgtaggtat gtacgcgccg caagacaaaa aagaacataa aaaagtgaga taaatagacc
     6121 gtctgttctt gtgatacgcg atagcgtatc agaatacctt tattcaatag tcaaaataac
     6181 cgctctgttg agcggttatt ttggcaaagc ggcacatact tacatcggca ttgatgtgtt
     6241 cggcgctgtg ggcgttgcct tgcgtttatt gagcgccgcg tagagggccc atagaccgat
     6301 tactaaatgc aagatgagat cggcaggcga ttccagatta gctccgagaa gcttcgtgcc
     6361 agctaaatta taaagtgctg ccaaaattgc aatgataccg acaacgatta caatcggctt
     6421 gtgcgttgaa gcaggaagca aaaaggctac gacgagcgcc acgattccga gaacaaaatg
     6481 tgcccagttt tcggccgtat cgaaccacca accgctgcca aatatgctgc tgtcagcagt
     6541 cggacccaac acattgatcc aaccaagaat tcctacgaca accagaatga ttccgccgat
     6601 aacaagaaat tgttttgaat tcatgaatga tttttttgtc cttcgatcca acttaggaca
     6661 accctgagtc ttttatcgaa gggttgagcg aagcgattag aacccgcccg gacgactggc
     6721 cgttcatctg ggcgggaatt tttaccgagc accacttttg aagcattgcg gccaaaagtg
     6781 gtgatcagtc tgaaaaaata tattcgctac gctcattatt tttttcgact tttattacaa
     6841 tagtatacgt atagatgtca aatgttgaaa gtggaaaaag gtcccttgtt ttgtgcctat
     6901 aaattgtcat attccaggct tttaaagaga tatattctgt gactaaagta tgctagattt
     6961 aatcttagaa aaacaaaaat actgccatga gcgttccagc tttacccacc ggttccgcgt
     7021 ttgaagtgag tcgaacccat gtcttttgta acccagagag gaagtgggtg caacccctga
     7081 gaggagtttt tttggatcat cgtattccag acagtattga tcttagttcc gttatggaaa
     7141 cggttcatga ggttaagctg gggatacgcg gtggagtgcg tcggattgaa gtctttccgt
     7201 accaatgggt ctgggatgat aattttgtgt cggtagcaat ttgctgccaa atctcaatca
     7261 atcctccttg ccaacttttt cttctttttt ccattcgaga ccgaaaactt gccacatttg
     7321 gcgggctcga agaatactga caggcttcta catgtattga agcctttttt cttttaaaat
     7381 agtaagatat gcattatgaa attacgggaa cttgctagaa aaaaatgtgt gccgtgcaaa
     7441 gggggaatga agccaattgg ttctccagaa gccaaaaatt atctcgcgga gcttcctctg
     7501 tggcagttgg acaaagatga aaagaaaatt tctcgggagt ttacatttga caactttgcg
     7561 aatgctatga tttttgtcaa ttgtgttgct cacattgcag aagaggaagg acatcatcct
     7621 gatatacata tttattacga caaagtaaaa ttgtcactta ctactcacgc tatagacggc
     7681 ttatcattaa atgactttat tttagctgct cggataatta cagactgtac ggataagcca
     7741 gaaattttgc aatgatctct acctccaaga tcaaagaacg agatgagccg gtaaccttta
     7801 tcatttttgg tgcaacgggt gaccttgctt tactcaaact tattccagcg ttgtttcgtc
     7861 tttttaaaaa gggctatttg tctagtgaat ttcgcattgt tggatttgcg cgccggccat
     7921 ttacgaatca ggagtatcga gcacttgttg cttccagtat gtcggaagaa accaaagtaa
     7981 cggaagacta tcagaaattt ttacaacaga tcgcctacca gcagggccag tttgacgaag
     8041 aaaaaacgta tcgtaacttg atgctcttgc ttgacgaact tgacgataaa atgggttcct
     8101 gctctcacaa gatttttcac tttgcaattt cacctttcta ctatgaaact gtgcttacaa
     8161 atctaaataa aacaggattg acgatttcat gtacggatgg atctggaaga accagagttc
     8221 ttttggagaa acccatagga acggatgggg aaagtgcgga acatatacag gctctgttgt
     8281 cagggatttt tagagaggac cagattttta gaattgatca ctatttggcg aaacgagggg
     8341 tgagtgattt tttaatgttt cgttatgaaa acccggcttt tgaaactgta ctcaatgctg
     8401 attttgttga agaagtattc atagaacaat ttgagacgcg cactatcgag gggcgcggtg
     8461 gtttttatga tcacattggt gcgcttcgag atgttggtca aaatcatttg cttgagattt
     8521 tggcactcat tgccatggaa cggccacgga aagattctgc ggaggctttt tcgttgatgc
     8581 gcgcccaggt gcttagcgag cttatcattc ctgactctgc agcgatcgaa cgcaaagtca
     8641 aaaggggtca gtataagggc tatcttgaag aagaaggtgt ggctccattc tcgaatacgg
     8701 aaacctattt tagaatacca gtttttataa aaagtcatcg ctggcgccgc gtcccatttg
     8761 tcatcacgtc tggcaaggca cttctcgaat cgcgtgcgac gatcaggatc atttttagac
     8821 ggaccgttga gtttaaatct ctggtcgaca aagaaacgag aaaagccgat atgcttgtct
     8881 tctcaatcca accggagtct cgcgtcgaat tctgtgagcg tggactcaaa agtactaggg
     8941 attcatatac ctgtgccatt tccacgtatg aagaagttgg cgtgccggca agtacgcctg
     9001 tagacgaata cgaacgtatt tacctagatg cgcttgcgga agataagtca atcttcgtca
     9061 gtcgaagcga ggtcaaagcc gcatggaagt acattgatgg tatagttatg gcatggcgag
     9121 aaaaagcgct cgtaatctac aaaaaaggct caaatccaga tgacttgtaa gcaataaaat
     9181 acttaaaaat gggcactatt tcagttaaaa catatcgcag aggcttggaa aaaaaagcgg
     9241 cacaagctgt gtcgatattg cttcataaat acaaaactaa accaatactg ctcatggttt
     9301 cgggcggttc tgcgcttaaa attttaaatc atacaattgc cgacactttc ggatctaaaa
     9361 taacggtgac tgtactcgac gaacgtttca gctctgatcc ggctgtgaac aattttctgc
     9421 aaattgccat ttctgaattt tacgaacagg ccaagaaaaa caacgttaat tttttgatta
     9481 cggttccgct tcccaatgaa cacatacaag acattgctga ccgttggcag tttgatatca
     9541 ggctctggct cgccaatcat ccgggcggga aagtggtggc gcttatggga atgggcgaag
     9601 atggacatac agccggaatc atgccctatc ctgaaaacga atggattttt aatgaacttt
     9661 ttgaagacga gaacaaatgg gttgtggcat atgacgcgct ctcaaaaaac cagcatcccc
     9721 agcgtatcac gacgactact catttcctga agcatgttat cgacgaggcg attttatttg
     9781 tgacaggtga acagaaaaaa gaaatgttgc gccgagcact tgcgcctgac ggcaaggtac
     9841 atgaatatcc ggtgcgcatt attcacgaaa tgaagcaggt cacggttttt acggatatta
     9901 ttttacaaaa ggtggacaat agaaaaaaaa ccgaggttaa tcagattaag gcgaactaat
     9961 ccaatatctg taaatgctaa aggaattttt aacttgtccc gttagagatt tcataaaaat
    10021 atcagtatgg gagtaaaaga ttggcttatc tctagcggga tgaagcagat gttgaatcag
    10081 cagctcaaaa atgtacccga agcagaacgg caaaaattag tttctttggt ggagaaaaat
    10141 ccggagtttt ttatgaagat tgccggagac atcaaagcaa aagtagatgc cggaaccggc
    10201 gagatgactg ccgccatgga agtcatgaaa gaaaatgaag ccgagctctg tgggatgatc
    10261 taaattcgcg aattatgaat tatcttatgc agtattggag ggtttaatca aagaggtctt
    10321 tttcaagctg catattcatt ttctttctta atctaatact atcaaggcag tccaatgcaa
    10381 attttttata cgcggggaaa gttgcgaatt gatcgagaac tatgtttttt ctgcagaatg
    10441 gatttttggt cgcgggatct agaaattcct cccaactgga tttcaccaac ttgggaaccg
    10501 agttcccaag ttggtgaact tcatcgttta aattaacgta agcactcaaa tgcaacaagt
    10561 attcatttga attaacatga acagccttat attttccctg gaatagagac ccggatcttt
    10621 tatgtcgctc gttgaaataa ttggtatatc cgagacctag tttgtgcatg aatttttgta
    10681 tgccggcatc atgttgtggc gttactataa agtgaaaatg atttggatta agacagtaag
    10741 ctgtgaactc tataagtttc ggtttgggaa ccgagttatc aggtttgtcg aatctatgct
    10801 cataaatgct tccaatagaa acgggcgtat tgaattcatt catgcttaga aaaaaacgat
    10861 ttatatcaaa aaaatcagca aagatatttc tcttatccac gcccctgttg taaatgtggt
    10921 aatactcacc taaagcaaat gaaattttcc gcataggtgc aattttatca cttgggaact
    10981 cggttcccaa gtgataaggg gataactaaa taggatttga tcttttttgt ttatataata
    11041 tctacattca ttaaaagatg agcgaaattg gtgttgaatc attatcgagc ccaggatgcc
    11101 acaactgcga ggcatttgaa cgcttctggg acggagtcaa gagccactat cccaacgtaa
    11161 agtatacgaa caaaagtatt ttgacaccgg aagggcagga aatggcgcaa aagttcggca
    11221 tttttgcgtc acctggaatt gtcataaacg gagagctttt ttcaaccgga ggagtaagca
    11281 ctgataaatt cattgcaaag atcaaagaac tctcgtcccg ttagagttta tgaaaataca
    11341 agaatattta ttcataaaaa acgaccagta ttattgattg aacagctttt ttaagacttt
    11401 aaactctaat aggattcata aaatgaccaa atggcttgaa aaaataagtg tcgaatgggt
    11461 tttgcggctt gggctcggcc tgacgcatct ctattcagga ctcgatctcg tgcgacatcc
    11521 ttctgcatgg acgtgggcgc ttcggccttt gcccgaattt atgaggacac ttataggttc
    11581 agtt
//
LOCUS       DATDSU010000165        12613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_1831356, whole genome shotgun sequence.
ACCESSION   DATDSU010000165 DATDSU010000000
VERSION     DATDSU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831356"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1946
                     /locus_tag="VI981_05685"
     CDS             66..1946
                     /locus_tag="VI981_05685"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479809.1"
                     /translation="MFLVNVIPIAKTGGKDELTYFSQNDIDLGSLVYVPLGRRTVPAV
                     VISKKKADSEKASIKSASFSIKKIEKTKSHPFFLPEFIEAVTEIASFFATSPGAVIYA
                     LTSNRVLENIDKIDYTRQERASNMHDLLLMQADDEDRFAQYRSLIREEFAKKRSVFFC
                     LPTIQEIKTSKSRLEKGIEQYTFVLHSGLSKKETLVVYKKILTEPHPVLVVATGNYLH
                     IPRADIGAYIVDRDASRAYRMITRPYIDIRKAVECVAKRMRCRAFFGDMFLPAETVYR
                     YKNHEFHDVVPLKSRLLTTAKLKLIDMKVYKNSARKFKILSDELVALIKETRNENGRL
                     FIFASRRGLSAITACGDCGTIVSCSTCGSSLVLHSSQSGNFFLCHKCGERRSPKELCR
                     NCTSWKLTPLGIGIELVRDAILELDNRPKLFVMDKDEIKSHQEATKRVQKFYDMPGSI
                     LLGTELALPYLTEAIEHTAVATVDALFAVPDYRINEKIIWILLKLRSLAKQTFLIQTR
                     KAGERLFQYAQSGNLADFYREEIELRKQFNYPPFSVLIKISIEASREKVVSEMAIVQK
                     IFADKEFEIFPSFIKGRRGMTLLHGLLRLQTETWPDPSVVSRLRALPPYFSVRIDPDS
                     LL"
     gene            2209..2580
                     /locus_tag="VI981_05690"
     CDS             2209..2580
                     /locus_tag="VI981_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479810.1"
                     /translation="MKKSIKMNTQSRRAQDRILTEFMRLAQEVERRDDGSPVRTVKNT
                     RLLARQLPLVTALREERIVQEIIHLGTRVRLNAAGVPILAEKDSRSKVNLGIMDVQRL
                     LMDLLIEVHARTFRYEPPHAA"
     gene            2665..3219
                     /gene="def"
                     /locus_tag="VI981_05695"
     CDS             2665..3219
                     /gene="def"
                     /locus_tag="VI981_05695"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY4479811.1"
                     /translation="MVNILQKDSPILRQKAKNVPASLFGTKELSRIVADMKTALHAEF
                     DGVGLAAPQIGVSYRIFIVAGRVLDSGDSLSETPALEIEPDWSRDLIFINPVLVKTSK
                     DKKLQEEGCLSVRPLYGTVRRASRAAVRAQNEKGVSFERGASGLLAQVFQHELDHLEG
                     VLFIDKASNIREIPLEETTETNLK"
     gene            3347..4204
                     /gene="fmt"
                     /locus_tag="VI981_05700"
     CDS             3347..4204
                     /gene="fmt"
                     /locus_tag="VI981_05700"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY4479812.1"
                     /translation="MSGRSPIKFAFFGTPAFAVKVLEELEKKGLLPAIVVTNPDRPQG
                     RSLRVLPSPVKSWAQKHKCAILQPETLDEQFIEHMRLAAMDVFVVAAYGKILPEKLIY
                     LPPRRSLNVHPSLLPKYRGATPIESAIIDDEHEIGVTIICLDKDMDHGPIVAQERAEL
                     KVWPMSAPLLEHLLAEQGGRLLARILSDWLFKKIRAVPQHHTLATYTKKIKKDDGLID
                     FNDNPRKNYLKYLAFDPWPGVYYWSSRGDRRKRIIIKKASFSDGEFIIERILPEGGRE
                     IPFSPEFKI"
     gene            complement(4208..4579)
                     /gene="raiA"
                     /locus_tag="VI981_05705"
     CDS             complement(4208..4579)
                     /gene="raiA"
                     /locus_tag="VI981_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY4479813.1"
                     /translation="MPITTNIKASNLTLTPSVADYVDKKLIRHIERFISRDDSSARAD
                     VELAKTSRHHNTSEEVFRAEINLHIGSMHLRAEAVNQDMFGAIDKLKDEILRELTSSK
                     KKRFALFKYGAQQIKKILRRE"
     gene            complement(4946..5158)
                     /locus_tag="VI981_05710"
     CDS             complement(4946..5158)
                     /locus_tag="VI981_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEY4479814.1"
                     /translation="MYIRIRVKTEQKVEKLSMVKNDLYDVSVKEKATQNRANRRIIEL
                     LGQQFKGRRIRIISGHHSPSKIIDIE"
     gene            complement(5189..5644)
                     /locus_tag="VI981_05715"
     CDS             complement(5189..5644)
                     /locus_tag="VI981_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY4479815.1"
                     /translation="MRTLQVTSSSFANTGIIPKLYTYDGKNINPPLRVDNIPSGTQTL
                     AFIMDDPDAPGGTWVHWIEWNMDTAGLESFSILEGRDPVGISGSGTSGHMFYEGPCPP
                     SGTHRYFFKFYALDTILELSAGSGKDALEQAMTGHIIGRGEIIGLYTRS"
     gene            5757..6275
                     /locus_tag="VI981_05720"
     CDS             5757..6275
                     /locus_tag="VI981_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479816.1"
                     /translation="MNAFRRAIFLQWDILLKDPRTRPLRAEELVFDKSLKSGLEFLQR
                     LKVLLFVVTVQEGLTDRSLSRREFEGMKRRLSHTFSVDDILVCPHSKDAHCLCRPPES
                     GLFVETARVCRFELSHSVVVSNRSEDDRAASRCGCTFLPIGDINSSTFVGRKTYLCVG
                     EALEAIRRIISR"
     gene            complement(6387..8558)
                     /locus_tag="VI981_05725"
     CDS             complement(6387..8558)
                     /locus_tag="VI981_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY4479817.1"
                     /translation="MEHTHTAREKEEVIYTCPMHPEIRQDKPGMCPECGMNLLPAKKK
                     KTDMDHSHKPDGQASGEGKHAGHSTAMFLRKFWVSLIFTIPVVLYADVLKTIFKWSLP
                     QFPGIEYMPFVLGSIVFFYGGWIFLAGALREIQGRLPGMMTLIGIAVSAAYVWSVYAV
                     FTGDEALFWELTTLITIMLLGHWLEMRAVSGAQGALKELSKLLPDTAEVIRPDGQANG
                     TGNKKTVTIPLSELVEGDIVLVRPGAKIPSDGKIVDGESDVNESMVTGESRPVSKKDG
                     DAVIAGTINGDGALKMTVTNIGEKTFLAGVMRLVAEAQASKSRLQMLSDRAAFYLTIV
                     AVSGGVITLVAWLLSGADVAFAVARLVAVLVIACPHALGLAVPLVASISTTKAANNGF
                     LVKQRLALEAARTIDTVLFDKTGTLTKGEFGVVEIWNLNGQSKDEILGLAASLDALSE
                     HPIAKAVVSKAKERGLTLSEPRKFEALKGKGVKAFIDEKEIMVGGPALLQSIEVEVPD
                     EISKQVGDAGKKGQTIIFTLKGRMLLGAMALADIIREESREAIKSLKAMGVRTAMITG
                     DSEDVAAWVSKELGIDEYFARVLPHQKSEKVKELQSRNMKVAMVGDGINDAPALTQAD
                     LGIAIGAGTNVAIESAGIILVRNDPRDIVKIINLSKMTYSKMIQNLWWAAGYNIVALP
                     LAAGVLAFKGILLDPAIAAVLMSVSTFVVAVNAVTLKRKQL"
     gene            complement(8567..9013)
                     /locus_tag="VI981_05730"
     CDS             complement(8567..9013)
                     /locus_tag="VI981_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479818.1"
                     /translation="MNKAIAILSVAALIGAFLVFYNPKPTPSAIKDNSSSSDTIQKWE
                     TKTDEQASVTVVVTPIDLSSQSTKWKFNVAMDTHSIELNEDMMESAVLMDEQGREYKP
                     VNWDGPTGGHHLEGVLTFKRVMPSPQSIELKITGVGDVTRSFTWQL"
     gene            complement(9230..9682)
                     /locus_tag="VI981_05735"
     CDS             complement(9230..9682)
                     /locus_tag="VI981_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479819.1"
                     /translation="MTNNIAKSIAYGTLASAVLFGVYFTVLTLISGWSFAQDQFADFW
                     YFIIALVAGFGIQIALYQYIKGLVQSGQGMGKVVGVSATTSTAAMISCCAHYLVNIVP
                     ILGITGLATFVAQYQINIFWVGLVFNLFGILFISNKIRKFRKHLPVPL"
     gene            complement(9685..9885)
                     /locus_tag="VI981_05740"
     CDS             complement(9685..9885)
                     /locus_tag="VI981_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479820.1"
                     /translation="MINIKSVFKFWKDKGGKNQIHVSKDPVCGMRSTEGIDFIHRSVK
                     YSFCSDHCRQRFEKNPEIYITK"
     gene            complement(9898..10530)
                     /locus_tag="VI981_05745"
     CDS             complement(9898..10530)
                     /locus_tag="VI981_05745"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011992951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY4479821.1"
                     /translation="MQNSYGLWPLVIFNSLVFIIFAFSFTKPKTSRDWRSLGAFSAFI
                     VALFTEMYGFPLTIYLFSGWLTKWIPGLDPFTHNSGHLLEDLLGWGGDPHFGPFHIAS
                     YILIIYGFSLLARAWGVLYKAQRNHTLATGGPYERIRHPQYVGFVIIMFGFLLQWPTI
                     LTVVMFPILVWMYTRLARSEERDAQKEFGAMWDEYAKKTPAFVPRTKKVF"
     gene            complement(10542..10820)
                     /locus_tag="VI981_05750"
     CDS             complement(10542..10820)
                     /locus_tag="VI981_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479822.1"
                     /translation="MHNHDKGNGNSTMWMMMICCVVPVLLIALFGLGGKSLGAPTWVV
                     IGGIGVMIIAHYFMMGRSHKHSGGGKSATDGDCDHKDKNDHSDHGCCH"
     gene            complement(10832..11098)
                     /locus_tag="VI981_05755"
     CDS             complement(10832..11098)
                     /locus_tag="VI981_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479823.1"
                     /translation="MEQLSQNKYGWKCVLGAEVVYVLCVLGGFLPLRSAKGIELHHNL
                     FETLPGFTWISFGSFIIGAIYMFVFAWIFASYFVWMHNSSLINK"
     gene            complement(11150..11806)
                     /locus_tag="VI981_05760"
     CDS             complement(11150..11806)
                     /locus_tag="VI981_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479824.1"
                     /translation="MENTTSENKTIREENKVSYVSLKFKKPKPLIMAIGVAVILIVVI
                     LFSAKGLFVAATVNGSPISRLSIINELEKQGGKQVLNTIINKKLIETELNKQKVSITE
                     EEVDEEIKKIEAQVASQGGTLTKELDAQNITMEDLKLQILTQKKIEKLLADKVAVIDT
                     EIDAYIKDSKATPPKNIKAEDFNKQISEQLKQQKFQQEAQKWVSDLTTSAKIKYYVNY
                     "
     gene            complement(11825..12046)
                     /locus_tag="VI981_05765"
     CDS             complement(11825..12046)
                     /locus_tag="VI981_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479825.1"
                     /translation="MKLSRLMHVVSVVAGFAGLVSFAAAIIGSNDNLVFGVTKVDALL
                     CAGVLILIAIWLQIATIHHMMLEKRGEII"
     gene            complement(12097..>12613)
                     /locus_tag="VI981_05770"
     CDS             complement(12097..>12613)
                     /locus_tag="VI981_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4479826.1"
                     /translation="FNPEAKLVSVNPEKRELQFENGKTANFDLLIFIPPHQGPKVVRD
                     SGLGNEAGWIPVDKETLKTKYDKVFAIGDVIAATVASGKPRPKAGVFAHYEAEVVVEN
                     IVRELQGLPADKKYDGRASCFLETGFGKAGFAYGNFYNVPAPAIKMKRPGRMWHWGKI
                     LFEKYWLWKWF"
BASE COUNT         3362 a         2881 c         2791 g         3579 t
ORIGIN      
        1 aaatacacgc aactttgcgt tgggtcgcgc tttatacttg atacctcata ctctatactg
       61 tgtctatgtt tttggttaac gttataccga ttgcaaaaac gggcggtaag gatgaactta
      121 cctatttttc tcaaaatgat atcgatctcg gttcactcgt ttacgtgccg cttggtaggc
      181 gcactgtccc agccgttgtc atttccaaaa aaaaagctga ttccgaaaaa gcttcgatta
      241 aatcagcttc tttttccata aaaaaaatcg agaagacgaa atcgcacccc ttttttcttc
      301 ctgaattcat tgaagcggta accgaaatag ccagcttttt tgcgacgtcg ccgggcgcgg
      361 tcatatatgc gcttacatca aatcgtgtgc ttgaaaatat agataaaatc gactacacga
      421 ggcaggagcg tgcctcaaat atgcatgacc tattgcttat gcaggctgat gacgaagatc
      481 ggttcgcaca atacagaagt ttgattagag aggaatttgc taagaagcga tcagtttttt
      541 tctgtctgcc gacgattcag gaaattaaga catcaaagtc gcggctcgag aaagggattg
      601 agcaatatac attcgtgctt cacagcggcc tttcaaaaaa ggaaacactc gtcgtatata
      661 aaaaaatcct gaccgaaccg cacccggtcc tcgttgtcgc tacaggtaat tatttgcata
      721 ttccgagagc cgatatcggc gcgtacattg tggatcgtga tgcgtcacga gcgtatagaa
      781 tgattaccag gccctatata gatatccgca aagctgttga atgtgttgca aagagaatgc
      841 ggtgtagggc gttttttggt gacatgtttc tgcctgccga gacggtttac cgctacaaaa
      901 atcacgaatt tcacgatgtt gtgccgctga aatctagatt gctaaccaca gccaaactga
      961 agctcataga tatgaaggta tacaaaaatt ctgcgaggaa attcaaaata ctttctgacg
     1021 aacttgtggc tttgatcaaa gagacacgaa atgaaaatgg aagactgttt atatttgcat
     1081 caaggagagg tctttccgcg attactgcct gtggagactg cgggacgata gtgtcctgct
     1141 caacatgtgg atcatcactc gtgctccata gctcacagag cgggaatttt ttcttatgcc
     1201 ataagtgcgg tgaacgacgc tctcccaagg aactctgcag gaactgcacg agctggaagc
     1261 ttacaccgct cggaataggc attgaattgg ttcgcgatgc tattctcgaa ctcgacaacc
     1321 ggcccaagct ttttgttatg gataaagacg agatcaagtc acaccaagag gccactaagc
     1381 gtgttcagaa attttatgat atgcccggta gcatactcct tggcacagag ctcgcacttc
     1441 cctaccttac cgaagcgata gagcacacgg ctgttgccac tgtggatgcg ctctttgcag
     1501 ttcctgatta tcgaatcaac gagaaaataa tttggatatt actcaagctc cgtagtctcg
     1561 ctaaacagac ctttttaatt caaacaagaa aggctggcga gcgccttttt caatatgcgc
     1621 agagcggcaa tttagcagac ttctatcgcg aagagattga actgcgaaag cagtttaact
     1681 atcccccgtt ttccgtactc attaaaattt cgattgaagc atcacgcgag aaagttgttt
     1741 cggaaatggc gatagtacaa aaaatatttg ctgacaagga attcgagatt ttcccatctt
     1801 ttataaaagg gcggcgaggt atgacccttc tccatgggct cttgagattg caaactgaaa
     1861 catggcccga tccctctgtt gtgtcgcgcc tgcgtgcact gccgccttat ttttctgtga
     1921 gaatagatcc tgattcactt ttgtaaaccc cgttagaagc tcacacgggg cattaaaccc
     1981 cgtgttagct aaaatcaaat tgcgttctgc ttctaacggg gtaaacgtat ttgtgcgatg
     2041 atggcttacc ctgaagtggg ctctaatctt acttgcccgc cttgtggggc aggccaaatt
     2101 cgaagaattg agcctaagct ctgcttaggt aagatgagat ctacaggaaa ggaattgaca
     2161 attctgtact gcgaggtagc ataccgaggg ctagaaaacg ggaagaccat gaaaaaaagc
     2221 attaagatga acacgcaaag ccgtagagca caagatagaa ttttgactga atttatgcgg
     2281 ttggcgcagg aagtggaacg aagagacgat ggttctcctg tcaggactgt gaaaaatact
     2341 cgtctgctgg cgagacagct tccgcttgtc acggcgttac gcgaagagcg cattgtgcaa
     2401 gagatcatcc atctcggtac ccgggtcaga ttgaacgctg caggagtccc tattctcgca
     2461 gagaaggatt cacgctcaaa agtaaatctt ggcattatgg acgtccagcg tctgctcatg
     2521 gatctcctga tcgaagttca tgcaagaacc ttcaggtatg aacctccaca cgcggcttag
     2581 aaccctgtac tgacacaatc gccaaaccgg cgatttttta attgatgaat taacaaataa
     2641 cgaacaaatg gtatactgtc aagcatggta aatattcttc agaaagattc gcccatcctt
     2701 cggcagaaag caaaaaatgt gccggcttca ctgtttggca ccaaggaatt gagtcgcatt
     2761 gttgcggaca tgaaaacagc acttcacgcc gagtttgacg gcgtggggct tgccgctcct
     2821 caaattggag tatcctatcg gatatttata gtagccggca gagttctcga cagcggtgac
     2881 tctctaagtg aaacgcccgc gctagaaatt gaacctgact ggtcgcgcga tctaattttt
     2941 attaatccgg ttcttgttaa gacttcgaag gataaaaaat tgcaggaaga gggttgtcta
     3001 tctgtgcggc cgctctatgg aacagtgcgg agggcttccc gggccgccgt tcgagcgcaa
     3061 aatgaaaaag gagtatcatt tgaaagaggt gcaagcggcc ttctagcgca agtctttcaa
     3121 catgaactcg accatctaga gggcgttttg tttatcgata aggcttcgaa tattagagaa
     3181 atcccgctcg aggagacgac cgagaccaac ttaaaataga cgatcggtag aaaataccgt
     3241 ttgagcgcca agaatattcg aatacaatta gtgccatgaa catgtctaca cctcaaaata
     3301 actacggcgg cgacgcgcat ctatatatgc ttttttttac taccccatga gtggacgaag
     3361 tcccattaag tttgcttttt ttggaacgcc ggcttttgcg gtgaaggttt tggaggaact
     3421 tgaaaaaaag gggctgttgc cggctattgt agttaccaat cccgatagac cgcagggacg
     3481 atcacttcgt gtcttgccgt cgcccgttaa atcgtgggcg caaaaacaca aatgcgctat
     3541 cctacagccc gaaacgcttg acgagcagtt tatagaacat atgcgccttg cggcaatgga
     3601 tgtttttgtg gtggcggcat acggaaaaat cttgccggaa aaattgatct atcttccacc
     3661 tcgacggagc ttgaacgttc atccgtcgct actgcctaaa tatcgcggcg caactcccat
     3721 tgaaagcgcc atcatagacg atgaacatga gattggggta acaatcatat gcctcgataa
     3781 agacatggat catggaccta ttgtggccca ggaacgggcc gaacttaagg tttggcccat
     3841 gtcagctccc ctactcgaac atctccttgc cgagcagggc ggcaggctac tcgcgcgaat
     3901 tttatctgac tggttgttta aaaagatccg agctgttcct caacatcaca ctctggcgac
     3961 ctacacaaaa aaaataaaaa aagacgatgg tctcatagat ttcaatgaca atcccagaaa
     4021 aaattatttg aaatatcttg cattcgatcc ttggcccgga gtatattatt ggtcctcgcg
     4081 gggtgataga cgcaaaagga tcattatcaa aaaggcctcg tttagcgacg gagaatttat
     4141 tatcgagcgt attttgcccg agggtggcag agaaattcca ttttctccgg aatttaaaat
     4201 ttagaaacta ttcgcggcgc agtatttttt tgatttgttg agcgccatac ttgaacagcg
     4261 caaaccgctt ttttttactg gacgtcagtt cacggagaat ttcatctttg agcttatcga
     4321 ttgctccgaa catatcttga tttaccgctt ctgcacgcaa gtgcatcgag ccgatgtgca
     4381 aattaatttc cgctcgaaac acctcctctg acgtattatg atgccgagac gttttggcca
     4441 actccacgtc cgctctcgca ctagagtcgt cacgcgaaat aaaacgttca atgtgtcgaa
     4501 ttaatttctt gtctacgtaa tctgcaaccg acggagtcaa cgttaggtta cttgccttaa
     4561 tgtttgttgt tatcggcata tacgagaatt agtaaataaa tctatattta gtatactccg
     4621 aagcaatctc ttcttccata cacatttatc ccagctagcc tttggggttt accccgttag
     4681 aagccgtgat tgctgatcgc gacctgtccc gttagaagtt tacccatatg atttattatc
     4741 actctagtct agtcaaacag gataaagttt gaagtgccac tttggaaata ggcaggactt
     4801 ctaccgggat ctgcttttgc gggatgaatg caatttgatt ttagagaaca cggggtttga
     4861 tgccccgtgt gagcttctaa cggggtttgc ggaaatatcc aaaaacctgg cccactgtag
     4921 ctataacaga gcaggtcaaa ttttgttact cgatgtctat gattttcgag ggtgaatggt
     4981 ggccgctaat gatccgaatg cgcctacctt taaattgttg gcccaaaagc tcaatgattc
     5041 ttctattagc tctgttttgc gtcgcctttt ccttgacgct aacatcatag agatcattct
     5101 tgaccatact taatttctcg actttttgct ctgttttaac gcgtatcctt atatacatag
     5161 ggttacatgt tttcgaatgc ccttttagct atgatcgtgt gtataatcct atgatctctc
     5221 ccctaccaat aatatgtcct gtcatcgcct gctcgagcgc atcttttccg ctacctgcgg
     5281 agagttcaag gatggtatca agtgcataaa atttgaaaaa ataacgatga gttcccgagg
     5341 gcggacaggg gccctcataa aacatgtgcc cacttgtacc gctcccactt atgccgactg
     5401 gatcacgtcc ctcaagaatt gaaaaacttt caagtcctgc agtatccata ttccattcga
     5461 tccagtgaac ccaggttccg ccgggagcat cgggatcatc cattataaag gcaagcgtct
     5521 gagtgccaga gggaatattg tccactctaa gcggcgggtt aatattttta ccatcgtatg
     5581 tatagagctt cgggataatg cccgtatttg caaatgaact gctcgtaact tgtaatgttc
     5641 tcatgtttat atattagcgc actttcgatt actatgtacg ctctatggtt gacattgctt
     5701 tcggttagcc caaaatagtg aaggatttga ataatcggta ctgcagaaca cgccctatga
     5761 acgcgtttag aagagcaatt tttcttcagt gggatatttt gcttaaggat ccgagaacaa
     5821 gaccgctgcg cgccgaggag ttggtattcg acaagagcct caaatcaggt cttgagttct
     5881 tacagagact caaagtcctt ctttttgtag tgacggtgca ggaagggctc actgaccgct
     5941 ccctttcacg acgagagttt gagggtatga agcggcggtt gtcccacacg ttttccgtcg
     6001 atgacattct ggtgtgtcct cactcaaagg atgctcattg cctttgtaga cctccagagt
     6061 ccggcctgtt tgttgaaact gcacgcgtct gtcgattcga gctctcgcat tctgtcgtgg
     6121 tcagcaaccg atcggaggat gatcgggccg caagccgttg cggatgcact tttttgccca
     6181 ttggagatat aaactcctcg acctttgttg gtcgaaaaac gtatttgtgt gttggagagg
     6241 cgcttgaggc cattcggcga ataatttccc gttgaaaacg cgaatccaca tcgcgttttt
     6301 tatttttgct agcgagcggc ggcatgaaag tttacgcaaa aaaaggtacg acgaaccgta
     6361 tggcttcgcg ctctgtaggc acttttttac aattgcttcc gcttgagtgt taccgcattt
     6421 acagccacaa caaaagtact taccgacata agcactgccg caatcgctgg gtccaagagt
     6481 attcccttga aagcgagtac cccggccgcg agaggcagtg cgacaatatt gtagccagcc
     6541 gcccaccaga ggttctgaat catttttgag tatgtcatct tggacaggtt gatgattttc
     6601 acaatatccc gtggatcgtt gcggacaaga ataattccag ccgactcaat agccacattt
     6661 gttccagccc caatggcgat accgagatcg gcctgtgtaa gcgcgggggc gtcattgatg
     6721 ccatcgccaa ccatggcaac cttcatgttg cgactctgaa gctcttttac cttttctgat
     6781 ttttggtgtg gcaatacgcg ggcaaaatat tcgtcaatgc cgagttcttt gcttacccat
     6841 gcggctacat cttcggaatc acccgtaatc atagcggtac gtacgcccat tgcctttaaa
     6901 gatttaattg cttctcttga ttcctcacga ataatgtcag caagggccat agctcccaag
     6961 agcatacgac ccttgagagt gaaaatgata gtttgtcctt tctttcccgc gtcgcctacc
     7021 tgttttgaaa tttcgtctgg aacttctacc tcgatactct gaagaagcgc agggcctcca
     7081 accattattt ctttttcatc aatgaaagct tttacgcctt tccctttcaa ggcctcaaat
     7141 ttcctcggtt cgctaagggt aagaccgcgc tcttttgctt tgcttactac agcttttgcg
     7201 attgggtgtt ccgacaatgc gtccaaagaa gcagcgaggc ccaatatctc gtctttgctt
     7261 tgtccgttta ggttccatat ttccacaact ccaaactcac cctttgtgag agttcctgtt
     7321 ttatcaaaca atacggtgtc aatcgtccgg gcggcttcta gagcaagtct ttgttttacg
     7381 agaaagccgt tgttcgccgc tttagtagtt gatatagacg caacgagagg cactgcaaga
     7441 ccgagagcat gcggacaggc aataacaaga acggcgacaa ggcgcgctac cgcaaacgca
     7501 acatctgctc cggataaaag ccaagctacc agcgtaataa ctcctccgct gaccgccact
     7561 atggtgagat aaaaggctgc gcgatcggaa agcatttgca atctggactt tgaggcttgc
     7621 gcttcggcga cgagacgcat gactccggca agaaaagttt tctctccgat atttgtgact
     7681 gtcatcttaa gtgcgccatc tccgttaatt gttcccgcga tgacggcgtc accatctttt
     7741 tttgacacgg gtcttgattc tccggtgacc atagactcgt tcacatcaga ttccccgtct
     7801 acaatttttc cgtcagaagg aatctttgcg cccggcctta caagcacgat atcgccctca
     7861 accaattcgg agagtggaat ggttactgtc tttttattgc ctgtcccgtt ggcttgtcca
     7921 tcggggcgga taacctctgc ggtatcggga agcagctttg acagttcttt gagcgctcct
     7981 tgtgctccgc ttaccgctct catctcaagc cagtggccga gaagcatgat ggtgataagc
     8041 gtggttagtt cccagaataa tgcttcgtct cctgtaaaaa ctgcataaac actccaaaca
     8101 tatgcagcag agacagcaat accgataagc gtcatcattc cgggtaaacg tccttggatt
     8161 tctctcaagg ccccagcaag aaatatccaa cctccataaa agaagacaat cgaacccagc
     8221 acaaacggca tatactcaat gccggggaat tgcggtaatg accatttgaa aatcgtcttc
     8281 aaaacatcgg cgtagagcac gacaggaata gtaaagatga gactcaccca gaatttccta
     8341 agaaacatcg ccgttgaatg acccgcatgt ttaccctccc cgctggcttg tccatcgggc
     8401 ttgtgcgaat gatccatatc ggtttttttc tttttggcgg gcaatagatt cataccacat
     8461 tcggggcaca tgccgggctt gtcttgcctg atctctggat gcatggggca ggtgtatata
     8521 acttcttctt tttctcgtgc cgtatgagtg tgttccataa ttatatttaa agttgccagg
     8581 taaaacttct tgtgacatca cctacacctg tgattttgag ctcaatagat tgaggagagg
     8641 gcattacacg cttgaaagta agcacgcctt ctaggtggtg gccgcctgtt ggcccatccc
     8701 agttgacggg cttgtattcc ctgccttgct cgtccatgag aacagcggac tccatcatat
     8761 cctcgttaag ttcgattgaa tgagtatcca tggcaacgtt gaatttccac ttggtagatt
     8821 gtgaggaaag atcaatcggt gtaacgacga cggtcacgct tgcttgttca tctgtttttg
     8881 tctcccattt ttgtatcgtg tcagatgagg aagaattatc tttaattgca ctaggagttg
     8941 gtttgggatt ataaaagaca agaaaggctc ctataagggc ggcgactgat aaaattgcta
     9001 ttgctttatt catcccgtta gaaacaggtc gcggtcgttt gaccaagacg ctggaaaatt
     9061 attttattaa atatattcga cattttattt attatgacta tcccacaagc gaccgcggtt
     9121 tctaacggga ttcatcccag ttgtaaaatc ttaattaata tattgtttat ttctcttttg
     9181 tttaattttg aaatacaatt cccgattctt ccttgtccgt acgttaaatt tacaacggga
     9241 caggtagatg ttttctgaac ttacggattt tgttgctgat gaacagtatg ccgaataaat
     9301 tgaaaacgag accgacccaa aaaatgttta tctgatactg ggcaacaaat gtggcgaggc
     9361 ctgtgatccc gagaataggc actatgttca ctaaatagtg agcacaacag gaaatcatgg
     9421 ccgcagtgga cgttgttgct gatacgccga ctactttacc catgccctgc ccgctttgaa
     9481 ccagaccttt aatatattga tagagtgcga tctgaattcc gaagcctgca actagagcga
     9541 taataaaata ccagaagtcc gcgaactgat cttgagcaaa actccatccg gagatcaatg
     9601 tcagtactgt aaaataaact ccgaataaga ctgcgcttgc gagtgttccg tacgcgattg
     9661 attttgcgat gttgtttgtc atgattattt tgtgatatat atttcagggt ttttttcaaa
     9721 tcgctgtctg cagtggtcgg aacaaaagga atatttgaca ctcctgtgaa taaaatcgat
     9781 gccttcagta gatctcattc cacacactgg atctttggac acgtggatct gatttttgcc
     9841 gcctttgtct ttccaaaatt taaaaactga tttgatgttg atcatatagt ttaggtgtta
     9901 gaaaactttt tttgtccggg gaacaaaggc cggagttttc ttagcatatt catcccacat
     9961 cgccccgaac tctttttgag catctctttc ttcacttcgg gcaagtcgtg tgtacatcca
    10021 cacaagaatt gggaacataa ctacggtgag aatggtcggc cattgcaaaa ggaaaccaaa
    10081 cataatgatc acaaaaccca catattgagg atggcgaata cgttcatagg ggcctccagt
    10141 tgcaagagtg tggttacgct gtgccttata cagcacaccc cacgcccttg cgagaagtga
    10201 gaacccgtaa ataattagaa tgtagcttgc aatatgaaac ggtccaaaat gtggatcgcc
    10261 gccccagcct aagagatcct caagaagatg acctgaatta tgagtaaatg gatcaagtcc
    10321 cggaatccat ttagtaagcc aaccagaaaa caaataaatg gtaaggggaa atccgtacat
    10381 ctctgtaaac aaagcaacaa tgaacgcaga gaatgcaccg agcgaccgcc aatcccgcga
    10441 ggtttttggc ttggtgaagc tgaatgcaaa tataatgaat acgagcgaat taaagatgac
    10501 gagcggccag aggccgtaag agttctgcat tgtagtctat attagtggca acacccgtga
    10561 tcagagtgat catttttgtc tttatgatca caatctccat cggtagcgga ttttcctcca
    10621 ccagaatgct tgtgtgatcg acccatcata aagtagtggg ctattatcat gacgccaatt
    10681 ccgccgataa caacccatgt cggagctccc aaggatttgc cgccgagccc aaagagagcg
    10741 atgagcagta cgggcactac acagcaaatc atcatcatcc acatcgtcga gttaccattt
    10801 cccttatcgt gattatgcat aggcgtttta gttatttgtt gataagactt gaattgtgca
    10861 tccatacgaa ataggaggca aaaatccaag cgaagacaaa catgtagatt gcgccgataa
    10921 tgaagctacc gaaactgatc cacgtaaagc cgggcagtgt ctcaaagaga ttgtggtgaa
    10981 gttctatgcc tttggcactg cgaagcggca gaaatccgcc gagtacacaa aggacatata
    11041 ccacctctgc tccgagaaca catttccacc catatttgtt ttgtgataat tgttccataa
    11101 atttattact aattgctgtt acaaattttt ggcggcattt atttagaagt taataattaa
    11161 cgtagtattt tatctttgcg ctcgtcgtga gatcggatac ccacttttgc gcttcttgtt
    11221 ggaatttttg ctgtttaagc tgttcactga tctgtttatt aaaatcttcg gccttaatat
    11281 ttttcggagg cgtggctttt gaatctttga tatacgcatc tatctctgta tcaatgactg
    11341 ccactttgtc ggcgagtaat ttttctattt ttttctgcgt tagaatctgc aatttcaagt
    11401 cttccatggt aatgttttgt gcgtcgagct ccttagtaag cgttccacct tggcttgcga
    11461 cctgcgcttc aattttctta atttcctcat caacttcctc ttccgttatg ctcacctttt
    11521 gtttatttag ttcggtctcg atgagcttct tatttataat cgtattgagg acttgcttgc
    11581 ccccctgctt ttcaagctcg ttaattatcg acagccgact gatcggactg ccattcactg
    11641 ttgctgccac aaatagtcct ttagcggaga acaaaatgac gacaataagt attacagcga
    11701 ctccaatagc cataataagc ggtttaggtt ttttaaattt taagctgaca taacttactt
    11761 tattctcctc tcgaattgtt ttgttttctg atgtcgtatt ttccatatat tttgagttaa
    11821 tctattagat gatctctcca cgcttctcaa gcatcatgtg atggatggtt gcgatttgaa
    11881 gccagattgc aatgaggata aggactccgg cacacaacag agcgtccact tttgtaacac
    11941 cgaacacaag gttatcgttg ctgccgatga ttgccgccgc aaaggaaacc aatccggcaa
    12001 acccggcgac aacactcaca acatgcatta atcttgatag tttcatattt cttagtgagt
    12061 taagttaatt gaataagaga tcgacctttt aatattttaa aaccatttcc acagccagta
    12121 tttctcaaat aatatcttgc cccagtgcca cattcttccc gggcgcttca tttttattgc
    12181 cggagctggt acattataga aattgccgta ggcaaaaccg gccttgccaa agcctgtttc
    12241 cagaaaacag ctggcacggc cgtcatattt tttatcggcg ggcaaccctt gtaattctcg
    12301 gacaatattt tccaccacca cttctgcttc gtaatgcgca aagacgccag ccttggggcg
    12361 aggtttaccg gaggcaacag tcgcggcaat cacgtcgcca atcgcaaaaa ctttatcata
    12421 ctttgttttc aaggtttctt tatcaaccgg gatccagccg gcttcatttc ctagtccgga
    12481 atctcttacc acttttggtc cttgatgggg tggaataaaa atgagcaaat caaaattggc
    12541 ggttttacca ttttcaaatt gaagttctct tttctcggga ttaaccgaga ccagtttcgc
    12601 ttcaggatta aaa
//
LOCUS       DATDSU010000166         6272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_2332673, whole genome shotgun sequence.
ACCESSION   DATDSU010000166 DATDSU010000000
VERSION     DATDSU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332673"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VI981_05775"
     CDS             <1..349
                     /locus_tag="VI981_05775"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY4479827.1"
                     /translation="KKLKKTFRFEIGVHLRTMDQVVKMLNAKPFKDTKITPDTRLYVT
                     FVQDGPKNKIKTPHISPDKSLQILKITGGEVLSILDLEKNGTSDAMNILQKIFGKNIT
                     TRNWNTIVKLAAE"
     gene            403..1362
                     /locus_tag="VI981_05780"
     CDS             403..1362
                     /locus_tag="VI981_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4479828.1"
                     /translation="MQSDFAIEIKGLTKSFKNIRVLKGINLDVKRGTMLALLGPNGAG
                     KTTTVRILSTLLRPDSGDVRVNGFDVVKNPKEVRRTIGLTGQFAAVDEILTGRENLEM
                     MGRLYHLGKSASGRKADELLATFDLVEASNRAVKTYSGGMRRRLDLAASLVATPPIVF
                     LDEPTIGLDPRSRLAMWDIIENLVKQGSTVLLTTQYLEEADKLADRIAVLDRGKIIAE
                     GSADELKSRVGKERIEITIDQNSDFEKAVSLIHGEAVKQKKEDRSISLSTDGSVREVK
                     RILDTLYSASIEIESMSVHKPTLDDVFLHLTGHATEAAELESK"
     gene            1364..2170
                     /locus_tag="VI981_05785"
     CDS             1364..2170
                     /locus_tag="VI981_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4479829.1"
                     /translation="MKVNEIPIENGIFTRLHWGIHDSWILIVRSLKHIFKNPDQLLGL
                     TIQPVMFMLLFGYVFGGAIETVGVSYINFLVAGILVQSSAFGATTTSLSVATDVKLGI
                     IDRLKSLPIVSSAVMTGHVTADLVRNVISSAIMVGVALVMGFRPSASFTEWLLILAIL
                     LLFTFAFSWISAIMGLLAKSIEAVQWMSFFIVFPLTFASAAFVPTESMPLVLRAFAQN
                     QPVTHVIESIRALMNGTPVGDHALLAIVWCTGLIVIAFPIASYLFRSRMR"
     gene            complement(2241..3314)
                     /locus_tag="VI981_05790"
     CDS             complement(2241..3314)
                     /locus_tag="VI981_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479830.1"
                     /translation="MPFKKSPCGTWVPARTYPLGSAFSAGDWNQGPGGGRFVPAMRKE
                     IDMAEKLENLKMVGATHIEFHNTEAPASEAPRLMKLVTDAGLKVGMVTANLFRLPQFV
                     GGNLGHPKNEVRKAAIEATCAYIAAGIEVFDADVYVYWNGSSGVNVPLGKPYRQTYLS
                     IAESLTGIVRWMVKTYGAERALPICVEPKFNEPPGWGMPADAGEVLAIIALMPEDARP
                     FVGTNIETCHSKIGMKRFAMELALSAASGRNFHIHLNDGSGAKFDEDRPFGFDWATAV
                     ETVYTLTEMKYDHLVGIDVQPFPADTGYQQADSIATSVREFRLAQRAIKHIDADILQR
                     LQSRDDDTHILRIFNHAQRMAID"
     gene            3587..4792
                     /locus_tag="VI981_05795"
     CDS             3587..4792
                     /locus_tag="VI981_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY4479831.1"
                     /translation="MIENSIVYRNFKNKYQYFPLGKAEGSLIWNREGKRFIDFTSAWN
                     VTNLGWNYPEVNEALIAQAKKNAQGLLWGSDPIQEAYAAVLTGALPCELDACVKETSG
                     TESIEASIKVARTYTNRKKIIGFSSGYHGQLFASLALAYYPEELKKLVPLVGEISPLL
                     FPHQDIGEEEYQKFLHDLENLLQKNDVAAIVTEPCIITVGGSTLVAYPGFLKSLRDLT
                     KKYGTLLIVDEVGTGFSRTGKLFGIEHEAVVPDMVVFAKAISNGVAAIGTVVGKKDIL
                     ESAFNDAVLISTFGWTPVACAAALKTLQVYMREKTWKMAAEKGVYIIKRLEKMIGGAI
                     IGVRGKGLEIGIQFRDALTCHEVQRKAYADGLHVVVGSRNNMQLMPPLTIPMELLDEG
                     LGILIKSFS"
     gene            4833..>6272
                     /locus_tag="VI981_05800"
     CDS             4833..>6272
                     /locus_tag="VI981_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY4479832.1"
                     /translation="MKKSFFSKTRTWVIGLILLAILAFVAVSFFRGGEEIEVLEVSEG
                     HFIQQISVSGKVEATDDVDLGFTQGGRVSHVYIRVGDQARAGALLAEIENGELRASVL
                     QKEAVVLVRQAKLKSLLAGTRPEQIAITKSKVESDRQAYTQSKEVLVDELRNAYTVSD
                     DAVRNQVDQFISNPRVSPKLNFVANDQGLQNRIQETRLVVERSFEAWLQNLTAIKDSI
                     DLRPYVSEAEKNLELTASLLRDASIVLQDPSRPASFSEYVADVSIARTKVNAAITSLT
                     SAITAQKNADTALETSKRNLALEEAGATEADVEAERAEVKAAEADLLNAQAQLLKTRV
                     IAPFTGIVTAVDAKVGQTIATGARAVSMMGSGAFQIEVFVPEINISVLEIGDKATVTL
                     DAYGENTPFGARVISIDPAETIRDGVSMYRSILEFIKQDSRIKAGMTANILITTEEKV
                     NVISVPQGVVKEKKGKKYIDVIEEGTITER"
BASE COUNT         1699 a         1400 c         1577 g         1596 t
ORIGIN      
        1 aaaaaaatta aaaaaaacat tccgatttga aattggcgtg catctgcgga cgatggacca
       61 agttgtgaag atgctaaacg ctaaaccatt taaagatacg aaaattactc cggatacacg
      121 tttgtacgtc acgtttgtac aagacggacc aaaaaataaa attaaaacac cgcatatttc
      181 ccctgataaa tcattgcaaa ttttaaagat caccggtggg gaagtgttga gcatattgga
      241 tcttgaaaaa aacggcacta gtgacgcgat gaatatactt caaaagatat tcggcaaaaa
      301 tatcacgacg cgaaattgga atactattgt taaattagcc gcagagtagc agaagatgta
      361 tgatcgagta gccttctcga taaattttag tatactcaaa ctatgcagag tgactttgcg
      421 atagaaataa aaggcctgac taaatctttc aaaaatatca gagtccttaa aggaataaat
      481 ctggacgtca aacgcgggac gatgctcgcg cttctcgggc cgaatggggc cggaaagaca
      541 acgacggtgc ggattttaag tactctcctt agacctgaca gtggagatgt gcgtgttaat
      601 ggttttgacg tcgtcaagaa tcctaaagaa gtgcgtcgaa cgatagggct caccggtcag
      661 tttgcggcag tggacgagat tttaaccggc agggaaaatc ttgaaatgat gggaaggctt
      721 tatcatttgg gaaaatcggc aagcgggaga aaggcagatg aattacttgc aacatttgac
      781 ttagttgaag cctcaaaccg cgccgtaaaa acctattccg gcggcatgcg ccgccgtctt
      841 gaccttgccg ccagtctcgt tgccactccg cccatcgttt ttctcgacga gccgaccata
      901 gggcttgatc cgcgaagccg tcttgccatg tgggatatta tcgaaaatct ggtgaagcag
      961 ggatcaaccg tgcttttgac gacgcaatat cttgaagagg cagacaaact ggccgaccgg
     1021 attgccgtgc tggatcgtgg gaaaattatt gctgaaggaa gcgcagatga gttgaagtca
     1081 cgggtcggaa aagagaggat tgaaatcacc atagatcaga acagcgattt tgaaaaagcg
     1141 gtaagcctca tccacgggga ggcagtcaag cagaaaaaag aagatcgaag catcagtctt
     1201 tcgaccgacg gaagcgtgcg tgaggtcaag cgtatactcg acacgctcta cagcgccagt
     1261 attgaaattg aaagtatgtc cgtccacaaa ccgacacttg acgatgtgtt cctccatctt
     1321 accggacacg caacagaagc ggccgagcta gaaagtaaat aacatgaaag taaacgagat
     1381 acctatagaa aatggaattt tcacgagact gcactgggga attcatgatt catggattct
     1441 gattgtccgc agtttgaaac atatatttaa aaaccccgat cagcttttgg gtcttactat
     1501 tcaacctgtg atgttcatgc tcctgttcgg atatgttttt gggggagcga ttgaaacagt
     1561 cggcgtaagc tacatcaact ttcttgttgc aggaattttg gttcagtcat cggcatttgg
     1621 agccaccacg acgtcgctct ccgttgcaac cgacgtaaaa ctgggaataa tagataggct
     1681 taaatctctg ccaatcgtaa gttcggctgt catgacagga cacgtaacgg ccgatcttgt
     1741 caggaatgtg atttcgtcgg ccataatggt cggagtcgcc cttgtcatgg gattccggcc
     1801 gtcggcttcg tttacagagt ggctcttgat cctcgcaata ctacttttat ttaccttcgc
     1861 attttcttgg atctcggcaa ttatgggact cttagcaaaa agtattgaag cggtacagtg
     1921 gatgagtttt ttcatagttt ttccacttac gtttgcaagc gcggcttttg taccgaccga
     1981 aagtatgccg ctcgtgcttc gcgcttttgc ccaaaatcag ccggtcactc acgttattga
     2041 atcaatacgt gctctcatga atggaacacc tgtcggcgat catgcactgc tcgctattgt
     2101 ttggtgcacc ggtctcattg ttatcgcttt tccaattgcc tcctatttat tcagaagccg
     2161 catgagatag tcttcccaaa tttcttaatt ttggtaaaaa aaccacgcag gtgcgtggtg
     2221 gcttagagaa ctatggggcg ttagtcgata gccatacgtt gagcgtgatt gaagatccga
     2281 aggatatgag tgtcgtcgtc gcgtgattgc aagcgctgga gtatgtcggc atcaatgtgc
     2341 ttgattgctc tttgagccaa cctgaactct ctcacgcttg ttgcgatcga atcggcctgt
     2401 tggtagcccg tgtccgccgg aaagggctgt acgtcaatac cgacgagatg atcgtatttc
     2461 atttcagtga gagtgtagac cgtctccacg gccgtcgccc agtcaaagcc gaatggtcgg
     2521 tcctcgtcaa acttggctcc agagccgtcg ttcaggtgaa tgtggaagtt ccgacccgat
     2581 gccgccgaga gcgcaagctc catggcgaaa cgtttcatcc cgatcttcga gtggcatgtc
     2641 tcgatgtttg ttccgacaaa tggccgcgca tcttcgggca tgagagcgat gatggcgagg
     2701 acttcgccag cgtcagccgg cattccccaa ccgggaggct cattgaactt tggttccaca
     2761 caaatgggaa gagctcgctc ggcaccatag gtcttgacca tccagcgtac gattccagtc
     2821 aggctctccg cgatagagag gtacgtttgt cggtatggtt tgccgagggg cacgttaacg
     2881 ccgctcgaac cgttccagta cacatagacg tcggcgtcga aaacctcgat gccagcggca
     2941 atgtaggcgc acgttgcctc gatggcggct ttacgaacct cgtttttcgg atgtcccaaa
     3001 ttgcctccca caaactgtgg caatcggaag agattggctg taaccatacc gaccttgagc
     3061 ccggcatcgg taacaagctt catcagtctt ggtgcctcgc ttgccggagc ctcggtatta
     3121 tggaactcaa tgtgcgtcgc accgaccatt ttgaggttct cgagtttctc ggccatgtct
     3181 atttcctttc gcatagccgg gacgaaacgt ccgcctcccg gtccttggtt ccagtcgccg
     3241 gccgagaagg cacttccgag tgggtaggtt cgagcgggaa cccacgttcc gcatggactc
     3301 ttcttaaagg gcatacgttg cctttcattt gttggcgtgt gtgcggtgaa ctattcacga
     3361 agtacgccga acttcgcgaa ttagactact ggctatacta tctgctttag gataatcgtc
     3421 aagaaaaaca agtatactaa cttcatgcta tggcacgaag ttagtatact tgttttttga
     3481 tttcaaaacg cagaatttga cggctctctc aagaaaatga ttttgagatg ttaaaaaaag
     3541 acgccgcact ctcgcaattt caatatttgt ggattaaaat ttggctatga tcgaaaacag
     3601 tattgtctac agaaatttta aaaataagta tcaatatttt ccgctcggga aagcggaagg
     3661 ttcactcatt tggaatcgtg aaggaaaacg gtttatcgat ttcacttcag cttggaacgt
     3721 tactaatctt ggctggaatt accccgaggt aaacgaggcg ctcatagcgc aggcgaagaa
     3781 gaatgcacag ggcctcctct ggggatcgga tcccatacaa gaagcatatg ctgccgtact
     3841 tacaggcgca cttccctgcg aacttgatgc gtgcgtcaaa gaaacgagcg gcacggagtc
     3901 tattgaagct tcaataaaag tcgcccgcac ctacacaaac agaaagaaaa ttatcggttt
     3961 ttcgagcggc taccacggcc agctttttgc ttcgctagcg cttgcgtatt accccgaaga
     4021 acttaaaaaa cttgtgccgc tcgtgggaga aatatcgcct ttgttgtttc cgcatcaaga
     4081 tatcggggag gaagaatatc agaaatttct gcacgattta gaaaaccttt tgcaaaaaaa
     4141 tgatgtggcg gcaattgtga ccgagccatg cattattaca gtgggtggtt ctacgcttgt
     4201 tgcctatccg ggttttctca aaagtttacg cgatctcacc aaaaagtatg gaacgcttct
     4261 cattgtggac gaagtcggaa cgggtttttc ccgcacagga aaacttttcg ggatcgaaca
     4321 tgaagctgtt gtgcctgaca tggttgtttt tgccaaggca atttcaaatg gagtggcggc
     4381 cattggtacc gtagtcggga aaaaagatat tcttgaatct gcatttaatg atgcggtatt
     4441 gatttccacc tttggatgga ctcctgttgc gtgcgcggcc gccctcaaaa cactgcaagt
     4501 ctatatgcgc gagaagacgt ggaagatggc ggcggagaaa ggtgtgtaca tcatcaaaag
     4561 acttgaaaaa atgatcggcg gcgccattat aggcgtccgc ggcaaaggat tggaaattgg
     4621 tattcagttt cgagacgcgc ttacttgcca tgaagtgcaa agaaaagcct atgccgacgg
     4681 acttcatgtc gtcgttggca gtagaaacaa catgcagctc atgccgccgc tcactatccc
     4741 gatggaattg ctcgacgagg gactcggcat ccttattaaa agtttttcgt aattttctcg
     4801 acgtttaggg cgaatctgct atactctgcc gcatgaaaaa gagctttttc tcgaaaaccc
     4861 gtacgtgggt aattggcctt attttattgg ctattttagc ttttgtagcc gtgtctttct
     4921 tccgaggcgg tgaagaaatc gaagtgctcg aggtgagtga agggcacttt attcagcaga
     4981 tttcagtatc aggaaaagtt gaggccacag acgatgtgga ccttggattt actcaaggcg
     5041 gccgcgtctc ccacgtttat atacgggtag gtgatcaagc acgtgccggc gcacttcttg
     5101 ctgaaattga gaatggagaa cttcgagcat ctgtccttca gaaagaagcg gttgtgctcg
     5161 tgcgtcaggc aaaactcaaa tctcttctag cgggtacgcg tcccgagcaa attgctatta
     5221 cgaaatcgaa agttgagagc gacagacagg cgtacactca atcgaaagag gtgctcgtcg
     5281 atgaattgcg aaacgcgtac acagtgagtg acgatgcggt tcggaatcag gttgatcaat
     5341 ttatcagcaa tccgcgagtc agtccaaaac ttaattttgt cgcgaatgat caagggctgc
     5401 aaaaccgtat ccaagagaca cgacttgtcg tggaaaggag ttttgaagca tggttgcaaa
     5461 acttaacggc gattaaagac tcgattgacc ttcgtcctta tgtctctgaa gcggaaaaaa
     5521 atttggaact taccgcgtct ttacttagag atgcaagtat tgttcttcag gatccttctc
     5581 ggccagcgtc tttttcggaa tatgtcgccg acgtgtcaat tgctaggaca aaagtaaatg
     5641 ccgccattac ctcgcttacg tctgccatca ccgctcaaaa aaacgcagac acagcgctcg
     5701 aaacatcaaa acggaatctc gccctcgaag aagctggggc gactgaagcg gatgttgaag
     5761 ccgaaagggc tgaagtgaaa gcggcagaag cggaccttct aaatgcgcaa gctcaacttt
     5821 tgaaaacgag agttatcgcg ccatttacgg gaatcgttac ggcagtggac gcaaaagtcg
     5881 gccaaacgat tgcgacaggt gcgagagcag tttcaatgat gggcagcggt gcatttcaaa
     5941 tcgaagtctt tgtgccggag ataaacatct ccgtgctcga gataggagat aaagcaaccg
     6001 taacacttga cgcgtatggt gaaaacactc ctttcggagc ccgtgttatc tcgatagatc
     6061 cggcggagac tattcgcgat ggcgtttcta tgtatcgctc aatcctcgaa ttcattaaac
     6121 aagatagtcg gataaaagct ggcatgaccg ctaacattct aattactacc gaagaaaagg
     6181 tgaacgtaat cagtgttccg cagggagttg ttaaagaaaa aaaaggtaaa aaatatattg
     6241 acgttatcga ggaaggaacg atcacggagc gt
//
LOCUS       DATDSU010000167        10179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate SMAG_U8011
            k141_160894, whole genome shotgun sequence.
ACCESSION   DATDSU010000167 DATDSU010000000
VERSION     DATDSU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962775
            Sequence Read Archive: SRR3989338
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (soil metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 22:34:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,171
            CDSs (total)                      :: 1,112
            Genes (coding)                    :: 1,109
            CDSs (with protein)               :: 1,109
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 0 of 3
            Pseudo Genes (internal stop)      :: 3 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160894"
                     /isolate="SMAG_U8011"
                     /isolation_source="soil"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.534762 N 107.782602 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2185
                     /locus_tag="VI981_05805"
     CDS             <1..2185
                     /locus_tag="VI981_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY4479833.1"
                     /translation="IVRAIFVDVTKLQFSQGGSTITQQVIKNALLNKDKKISRKLKEW
                     VLALKLEKVMTKEQILGIYLNEVPYGGNLYGIEEASQAFFGKTAKDVSLAEASYLAAL
                     PQAPTYYSPYGNHREQLEARKNLVLAQMLANKFITQSEYDEAKKEKVTFAPIEERGIK
                     APHFVFYVKEYLEKKYGEEAIEEEGLKVITTLDYELQKKAQESVKKFALQNEKDFKAK
                     NAAMAGVDPKTGQILIMVGSRDYFDKEIEGNFNVTLAHRQPGSAFKPFAYAEAFEKGY
                     LPETVVFDLKTEFSTACTPQGEPRTAGADCYMPVNYDNTYRGPISLRNALAQSINIPA
                     IKVLYLAGLKDTLTLAKNMGIESLDDRERYGLTLVLGGGEVSLLDLTSAYGVFANNGI
                     RNPHQAILRVEDKNGTILEQYEKKEIDVLAPDVAATITDILSDNAARTPAFGAASPLY
                     FPGRAVAVKTGTTNDYHDAWIVGYTPSIALGAWVGNNDNKAMERSIARFIVAPMWNEV
                     MKEILSVMPAEEFEKPLTKDTTSMKPAIRGLWQGAQSYFIDKSSGKLATTYTPLELRE
                     ERVVREIHSILHWVDRENPLGPPPQNPANDPQYTYWETPIRAWAATQGPDESSSVIPN
                     AEDDTHRPEYAPQIKILHPTANQALPKDSATEIRVETGGKNPAARVEYYVNGTYIGFQ
                     DKTPFNFTFTPSEVESLTERNVLKVVVQDTNFMRGEQTLEFAITE"
     gene            complement(2188..2436)
                     /locus_tag="VI981_05810"
     CDS             complement(2188..2436)
                     /locus_tag="VI981_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479834.1"
                     /translation="MFNIESFFDKFRVLEQTLKKSKDVLAREIEKASGVEVKPEYIEV
                     KNQILYIKATPHLKTALLIKKEKILVALRAVAHIEDIR"
     gene            complement(2564..3664)
                     /gene="dnaN"
                     /locus_tag="VI981_05815"
     CDS             complement(2564..3664)
                     /gene="dnaN"
                     /locus_tag="VI981_05815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY4479835.1"
                     /translation="MKLECLKERVRDAVAQAEKITGKHLTLPVLKCVLLEAKNNTLIV
                     RATNLDLGVEFFIPAKVEKVGVNAVSGVVLYNFLQNLQNEKTVSFESKEGILLLEARH
                     NVASIKTAPHEDFPTIPKVETGKLIKMHSNDLLQGLKAVWYSSATTSMKPELSSIYIY
                     SDDVGFVFVATDSFRLAEKRIKQKKSMEVSGILIPHKNIPEIMKILESINGDVDVSLD
                     RTQIAIIHEGVYLTSRVIDGAFPDYKQIIPKDFKTEVVLLKQDLINALKLTNVFSDNF
                     NQVHFKINPTLKLFEISTKNMDIGENRNFVEAAISGEPLEINFNYKYIVDCFQSLTTD
                     SLSLKFNGLARPMVIEGIGDKTFLYLVMPMNK"
     gene            complement(3895..5331)
                     /gene="dnaA"
                     /locus_tag="VI981_05820"
     CDS             complement(3895..5331)
                     /gene="dnaA"
                     /locus_tag="VI981_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY4479836.1"
                     /translation="MNAVKIISLTRVGLKPGDDFLTHYRSRNKLIMIDNKKLWDSVLV
                     EMEMNISKANFNTWFKETSVLKQEEGTIYLSVPNAFVKDWLINKFHKNILKALRTYGD
                     NVRSIEYLVSKEGDKKREIEPKTAITYSSELPLHDFYINKEDNLNPRYTFENFVVGPF
                     NELAHAAAQAVIKKMGVVYNPLFIYGNTGHGKTHLIQAIGNTIKNANPARKIYYVTSE
                     KFTSDLVSALQNGKISQVKEKYRKYDVLIMDDIQFLSNKEKTQEELFHLFNTLYDNNK
                     QIIFSSDKHPNYIPNLEERLKSRFAAGMIVDISAPDHESRIAILRAKAKQSSFILNEE
                     IVDFLAQVVEGNIRELEGTLNSIICQSQLKGKDLNLIEVKNLIKNSAKPKKAISVKDI
                     IKIVADFYNIDEETIYDKTRKKEVVRPRQLAMYILREDCNVSYPSIGQKLGGRDHTTV
                     IHSCEKIKNELKIDHLLEQQLNQIRALM"
     gene            5681..5815
                     /gene="rpmH"
                     /locus_tag="VI981_05825"
     CDS             5681..5815
                     /gene="rpmH"
                     /locus_tag="VI981_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY4479837.1"
                     /translation="MSQTYQPKVRKRARSHGFLKRMSTRGGKNTLLRRRRKGRNRLAV
                     "
     gene            5754..6161
                     /gene="rnpA"
                     /locus_tag="VI981_05830"
     CDS             5754..6161
                     /gene="rnpA"
                     /locus_tag="VI981_05830"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY4479838.1"
                     /translation="MAAKTLYFDVDARAETGSPFSTFMLSRKQRLRREDVIRVISRGD
                     SRHFPSFSLKYERSHRPYHRFGVVVSKKVAKSAVERNKLRRMFYEILSEFAKKDGRGM
                     DAIIQVKINSNTALLKDSAQLVEFFQGLNKLLP"
     gene            6176..6931
                     /locus_tag="VI981_05835"
     CDS             6176..6931
                     /locus_tag="VI981_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEY4479839.1"
                     /translation="MSWLYNAFVYKPLYNGLIFLLNALPLYANAALAIILFTLIVKFI
                     LLPLSKAAIRTQIKMKELEPLMKAVKEKYKNDKNQQSLEIMKLYRENKLNPLSGFFLI
                     LIQIPIIIALYRIFLYGGLPEIHTELLYPFVHAPEPGSVSMHLFGFDILQKSPVFAII
                     AAASQYFQAKFSMPLPASQEKKEGFQHDLGKMMSVQMKFVFPIIVLMISLNLAAAVVL
                     YWITSNLFAVGQEILVRRRMLEEHENSKKLKSP"
     gene            6931..7416
                     /locus_tag="VI981_05840"
     CDS             6931..7416
                     /locus_tag="VI981_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY4479840.1"
                     /translation="MDLEETKKIIETFLHQMSITDATIELTIDDHGDTKCVINTASSG
                     MLIGKDGRNWSALSHLIKKIVAKKAGQEDLRITVDVNNYLAEKMQKIKSSVEMLAERA
                     RSLGAPVEMSPMNAYERLIVHSIFEHRPEFKTESTGIGPERRVVITYVGPGGASDLFT
                     G"
     gene            complement(7402..8667)
                     /locus_tag="VI981_05845"
     CDS             complement(7402..8667)
                     /locus_tag="VI981_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479841.1"
                     /translation="MNGKHATLLIIFILLIVFAGLGIFFFTLMRENKPVDVPTTEEKD
                     ITIDFGGRGTPPIAQPEPQGGQNNDNLSSSPLATLRKISSLPQAGAVAIDIENETEIL
                     FVDQATGHVFRGSTEDPSISRISNTTIPKITEALWLASGTGFIARYLDQDGKTIQSYS
                     ATLIPRATSTIEDTGENSVPFKIEGKFLPRNLKVLIPSESDDLIFYIPEGERSVGYTA
                     KNDETKRTEVFNSDLGEWQIAWPKATHLALTTKPSGTVRGTLYLHNLKTKVTEKIMDG
                     LGLTALINNSLSRVLYANPSEKEFGLKVLSIKDHAVTNLGSGGLPEKCVWSKKEDFVV
                     YCAIPKAIPGALYPDMWYQGIVSFNDTIWQLNLKNGSTIELADPEKETGEMMDAVRFF
                     LNGKEDSLFFTNKRDNTLWSISLKESARK"
     gene            complement(9012..9782)
                     /locus_tag="VI981_05850"
     CDS             complement(9012..9782)
                     /locus_tag="VI981_05850"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY4479842.1"
                     /translation="MDRTEPIKKSILKIVIGMLSMFFAFTHKTAAQADYTPLVDLTDT
                     GFFTSYELVPFLVGAFKLAIAAAGVLAVFRIVWGGFTYMTFDSIEGKKDGKEIISKAI
                     GGLILAMAAWLILYTVNKDVLQFKPLEYPKTDGPISYPVLPLPGASGANPPPSVNTVP
                     DRDLIYKYDEMMRQGYSATTDPALLTRERAKELAIQNGISVPSGADIRYTTFGSVPVN
                     ITVNGREVPYNGRQYTANEAASAYRHSAATGLVNPLSR"
     gene            complement(9769..>10179)
                     /locus_tag="VI981_05855"
     CDS             complement(9769..>10179)
                     /locus_tag="VI981_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4479843.1"
                     /translation="PSTSIQGIVDLDVVTTDNSLRVRDSITIPVTEPLLLLYEDDPIL
                     GIKQNRALIGSFLLKAPEVRFFAAPYWSSPDSSGFAYKWKQNEELLEGDTPFRTFGVE
                     ANSRGESQISVIFSNSSKFLQFSSARIKLLYGSN"
BASE COUNT         2854 a         2176 c         2206 g         2943 t
ORIGIN      
        1 cattgtccgc gcgatttttg ttgatgtaac gaaactgcaa ttcagccagg gaggatcgac
       61 cataacgcaa caggtcataa aaaatgcgct gctcaacaaa gataaaaaaa tatctagaaa
      121 actcaaagaa tgggtcctgg cgctgaagct tgagaaagta atgacaaaag agcaaattct
      181 aggaatttat ctaaacgaag taccctacgg cgggaacctc tacggaatag aggaggcaag
      241 tcaggctttt ttcggaaaaa ccgctaagga cgtatcgctt gcagaagcga gttaccttgc
      301 tgcccttccg caggctccaa catactattc tccgtatggc aatcaccgag aacaacttga
      361 ggctcgaaaa aatctcgtgc tcgcccaaat gctagccaat aaattcatta ctcaaagtga
      421 gtacgacgaa gcaaaaaagg agaaagtgac ttttgctcca atcgaggagc gcggtattaa
      481 ggcaccgcac tttgtctttt atgtgaagga atatctggaa aagaaatatg gcgaagaggc
      541 cattgaagag gaggggttaa aggtaatcac aacactcgac tatgagctac agaaaaaagc
      601 ccaagaaagc gtcaaaaaat ttgcactcca aaatgaaaaa gatttcaaag caaaaaacgc
      661 cgcgatggca ggcgttgacc ccaagaccgg tcaaattctc atcatggttg gctcgcgtga
      721 ttactttgat aaagagattg agggtaattt caatgtcacc ctagcccacc ggcagccggg
      781 ctctgctttt aaaccatttg catacgccga ggccttcgaa aaagggtacc tgccggaaac
      841 tgttgtgttt gacttgaaaa ctgaattttc gacagcgtgt acccctcaag gagagccgag
      901 aaccgctggt gcggactgct atatgccggt taactacgac aacacgtacc gcgggccgat
      961 ctcgctacga aatgccttgg ctcaatcaat aaacatacct gccattaaag tactctatct
     1021 tgctggcttg aaagatacac ttacgcttgc aaaaaacatg ggcatcgaga gcctcgacga
     1081 ccgagagcga tacggactca ccttggtgct tggcggcggc gaagtttcac tgctcgatct
     1141 tacgagcgca tatggagtat tcgccaataa tggcatccga aatcctcacc aggccatatt
     1201 acgagttgaa gacaagaacg gtacgatcct cgagcaatat gaaaaaaaag aaattgacgt
     1261 tctggcgccc gacgtggcag ccacgattac tgacatactc tctgataacg cggccagaac
     1321 gccggccttt ggagcggcga gcccacttta ttttccgggc agagccgtag cagtaaaaac
     1381 gggtactaca aacgattacc acgacgcctg gatcgtcgga tatacgccaa gcatcgcact
     1441 tggggcgtgg gtgggcaaca acgacaacaa agccatggag cgtagtatag cccgctttat
     1501 tgtcgcaccc atgtggaacg aggtcatgaa agaaattctg tcggtcatgc ccgcagaaga
     1561 attcgaaaaa ccgctgacta aagatacaac gtccatgaaa ccggccatcc gcggactctg
     1621 gcagggtgca caatcatatt ttatagataa atcatcaggg aaacttgcaa caacatatac
     1681 gccattagaa ttgagagagg agcgcgtagt acgtgaaata cattcgattc tccattgggt
     1741 tgatagggaa aaccctttag gtccgcctcc gcagaaccca gcaaatgacc cccaatacac
     1801 ctattgggag acgcccatcc gtgcttgggc agcgacacaa gggccagatg agagctcaag
     1861 tgttatacca aatgccgaag atgacaccca tcgtcccgag tatgctccgc aaataaaaat
     1921 actgcacccg acggcgaatc aagcattgcc taaagacagc gccactgaaa tacgggttga
     1981 aacggggggc aaaaatcctg cggcgcgtgt cgaatactat gttaacggaa cgtatatcgg
     2041 ttttcaggac aagacgccat ttaacttcac ttttacaccg agtgaagtcg aatctctaac
     2101 tgaacgtaat gttctcaaag tagtcgtcca agacacaaac tttatgcgcg gagaacaaac
     2161 attggaattt gcaattaccg agtgacacta acggatgtct tcaatgtgcg cgacagcgcg
     2221 aagtgcgact aagatttttt ctttttttat aagtagcgcc gttttgagat gtggtgttgc
     2281 tttaatatat aaaatttgat ttttaacttc aatatattca ggtttgacct ctacgccgct
     2341 agccttttct atttcgcgag ccaacacgtc tttgcttttt tttaacgtct gttcgagcac
     2401 gcgaaattta tcaaagaaag actctatatt aaacatgatg gattttgttg agcgccgcga
     2461 ctacagaata actgccgggc accacttttg attttttaaa atccaaatgg ccaaaagtgg
     2521 tgcccggtaa gaaaattata atcactgcgt tcattaattt tcatcattta ttcataggca
     2581 taacaagata cagaaacgtc ttgtctccta ttccctcaat aaccatcgga cgagcaaggc
     2641 cattgaattt taaagaaaga ctatctgttg ttaaagactg aaaacaatct acgatatatt
     2701 tatagttaaa atttatttca agcggttctc cactaatggc ggcctcaaca aaatttctgt
     2761 tttcgcctat atccatattt ttagtgctta tctcgaaaag cttgagcgtt gggttaattt
     2821 taaagtgaac ttggttgaag ttgtccgaaa atacgttcgt cagtttgaga gcattaataa
     2881 gatcttgttt taaaagcaca acctcggtct tgaaatcttt cggaataatc tgtttataat
     2941 caggaaaagc cccgtcaata acacgcgagg tgaggtaaac tccttcatgt atgatggcaa
     3001 tttgtgtccg atcaagactg acgtcaacgt cgccgttgat tgattcgagt attttcatta
     3061 tttcaggaat atttttatga gggataagaa ttccgctcac ttccatactt ttcttttgtt
     3121 taattctttt ttctgccaac ctaaaggaat ctgtcgctac aaatacgaag ccgacatcat
     3181 ctgaataaat gtagatggaa gaaagttctg gtttcatgct agtggtcgca gagctatacc
     3241 aaacggcctt taatccttga agtaaatcat ttgaatgcat cttaatcagt ttacctgttt
     3301 cgaccttagg aattgtcggg aagtcttcgt gaggggcagt tttgattgag gcaacattat
     3361 gtcgcgcctc aagtagtaag attccttctt tgctttcgaa tgaaacagtt ttttcgtttt
     3421 gaagattctg tagaaaatta tagagaacca cgccagatac agcatttacg ccaactttct
     3481 caactttggc gggaataaaa aattctactc ccaaatcaag attcgtcgcg cgaacgatga
     3541 gtgtgttgtt ttttgcttca agtagcacac acttcaaaac tggaagtgtt aaatgtttac
     3601 ctgtaatttt ttctgcttgc gcaacggcgt ctcgcaccct ctccttcaaa cactccagtt
     3661 tcatataatg ttctttttaa atctattact attgttatat ctgtggatat ctcaaatttt
     3721 ctattataca gccttgaata aggttccccg atttttttta tgttgcggat agtgtgcaaa
     3781 caatttgata agctgtgaaa aataaattga cctgttccaa aatttttaat ttatcaccat
     3841 actgtacaca ctcttttcga aagttctcaa caccaaatcc ccaactttgt tctcctacat
     3901 aagcgccctg atctggttca gttgttgttc aagaaggtgg tcaattttca attcattttt
     3961 tattttttca catgaatgga tcactgtagt atggtcgcgc cccccgagtt tttggccaat
     4021 tgacgggtaa gaaacgttgc aatcttctcg gagtatatac atagccagtt gtcttggccg
     4081 gaccacctct tttttgcgtg tcttatcata gatagtttct tcatcgatat tgtagaagtc
     4141 agcaacgatc ttaatgatat ctttaaccga aattgctttt ttgggtttcg cactgttttt
     4201 tatgagattt ttaacctcaa taaggtttag gtctttgcct ttaagctggc tctggcaaat
     4261 tatcgagttt aaagtgcctt caagctcacg aatgtttccc tcgactacct gtgccaagaa
     4321 atcgacaatt tcttcgttca aaataaaact actttgcttc gctttagcgc gaagaatagc
     4381 gatgcgagat tcgtggtctg gggcacttat gtcgacaatc atacctgcgg caaagcgtga
     4441 tttgaggcgt tcttcgaggt tgggaatata atttgggtgt ttgtcggaag aaaagataat
     4501 ctgtttgtta ttgtcgtaaa gcgtgttaaa tagatggaaa agctcttcct gggtcttttc
     4561 tttgttagat aagaattgga tgtcgtccat aatgagcacg tcatattttc tatatttttc
     4621 tttcacctga ctgatttttc cattctgaag agcagacacg agatcggagg tgaacttttc
     4681 tgatgtaacg taataaattt tccgcgcagg gttggcgttt tttatagtgt tgccaatagc
     4741 ttgtataaga tgagtcttgc catgcccggt atttccataa ataaaaagcg gattgtacac
     4801 gactcccatt ttttttatca ccgcttgggc agctgcatga gcgagttcgt taaacgggcc
     4861 gaccacaaag ttttcaaacg tgtatcgcgg attgaggttg tcttctttgt ttatataaaa
     4921 gtcatgtagc ggcaactcgg aagagtacgt tatcgctgtt tttggctcta tctctcgttt
     4981 tttgtctcct tcttttgaaa caagatactc tatagaccgc acattatcac cgtatgttcg
     5041 cagtgctttt aatatatttt tatggaattt gtttatcagc cagtctttta caaaagcgtt
     5101 tggaaccgaa agataaattg tgccttcttc ttgttttaaa acagaagttt ctttaaacca
     5161 tgtgttaaaa ttagccttcg atatattcat ctccatctcg accaagacac tatcccaaag
     5221 ttttttgttg tcgatcatga ttaatttatt tctcgaacga tagtgtgtga gaaaatcgtc
     5281 gcccggtttc aaaccgacac gggttaagga aattatcttc actgcgttca ttaatttcct
     5341 ctaattatat acacaataga attgagcggt agttgccaaa acaaatcaaa tttgttcata
     5401 agctgttcat aacgtgttga cagtaaattc caaaattttt tttcgatcat gtaaaccccg
     5461 ttagaagctc acgcggggca ttaaaccccg tgttctctaa aatcaaattg cattcatccc
     5521 gttagaagca gatcgtgatc ggcgatcgtg gccttaaacg gggcttatcc ctacggcttg
     5581 gtgcggggcc cctgcttcta acagggtaca gctttagcct gcgtatattt gggatttcac
     5641 agatttggca aactgcttgt catactctat actcgaggtt atgtcacaaa cataccaacc
     5701 caaagtgcga aaacgtgccc gaagccacgg cttcctaaaa cgaatgagta cacgtggcgg
     5761 caaaaacact ctacttcgac gtagacgcaa gggccgaaac aggctcgccg tttagtacgt
     5821 tcatgctttc tcgaaagcag cgacttagac gagaagatgt aatccgtgtt atttctcgtg
     5881 gagatagccg gcatttcccg tcattttcac tcaaatacga gcgttctcat agaccatatc
     5941 accgttttgg tgttgttgtc tccaaaaaag tagccaaaag cgcagtcgaa cgaaacaaac
     6001 ttcgaagaat gttctatgaa attttgtcag aatttgctaa aaaggacggc cgggggatgg
     6061 atgcgattat acaggttaaa attaactcaa ataccgcgct cctaaaagac agcgcgcaac
     6121 tcgtagagtt ttttcaaggg ttgaataaac tattacccta gtctagaatg atgcaatgtc
     6181 ttggctttac aatgcttttg tttataaacc gctctacaac ggtcttattt ttttattgaa
     6241 tgcccttccc ctctatgcaa atgcggctct ggcgataata ctatttacac ttattgttaa
     6301 attcattctt ctgccgctgt caaaggcagc gatccggacg caaatcaaaa tgaaagaact
     6361 cgagcctctg atgaaggctg ttaaagagaa gtataaaaac gataaaaatc agcagtcgct
     6421 cgagattatg aaactctacc gtgagaataa attgaaccca ttgtcaggtt ttttcctaat
     6481 tttgattcag atccccatta ttattgcgtt gtatagaatt tttttatacg gaggcttgcc
     6541 cgagattcat acggaattac tctatccttt tgttcacgcg ccggaaccag gatcagtaag
     6601 tatgcatctt ttcggatttg atatacttca aaaaagtcct gtttttgcta ttatcgctgc
     6661 agccagccaa tattttcagg caaaattttc gatgcctttg cctgcctcgc aggagaagaa
     6721 ggaggggttt cagcatgatt tgggcaaaat gatgagcgta caaatgaaat tcgtgtttcc
     6781 catcattgta cttatgatct cgcttaacct cgctgccgct gttgtgcttt actggattac
     6841 aagcaatctt tttgccgtag gtcaggagat actggtacgc cgccgtatgc ttgaggagca
     6901 tgaaaattct aaaaaactaa aatcacccta atggatttag aagaaacaaa aaaaatcata
     6961 gaaacattct tacatcaaat gtcgattact gacgccacta tcgagcttac gattgatgac
     7021 cacggagaca cgaaatgcgt tatcaataca gcaagttcgg gaatgctcat tggcaaagac
     7081 ggccgaaact ggagcgcctt gagtcacctc attaagaaaa ttgtggcaaa aaaagcaggg
     7141 caagaagacc tgcggattac tgttgatgtg aacaactacc tcgcggaaaa gatgcaaaag
     7201 ataaaatcaa gtgtggaaat gcttgccgag cgtgcacgtt cactcggagc gcccgtcgaa
     7261 atgagtccga tgaacgcata tgaacgtctg atcgttcatt caatatttga acatcgtcca
     7321 gagtttaaaa cggagtcaac cggaatagga cctgaacgcc gtgttgtcat aacatatgtt
     7381 ggcccgggcg gggcaagcga tttatttacg ggctgattcc ttaagtgaaa tagaccataa
     7441 cgtgttgtct cttttgtttg tgaaaaagag agaatcttcc ttgccgttga gaaaaaacct
     7501 tactgcatcc atcatttccc cagtttcttt ttccggatcc gcgagctcaa tggtcgagcc
     7561 gtttttgagg ttcagctgcc aaatggtgtc gttgaatgaa acgatgccct gataccacat
     7621 gtccgggtaa agtgctccag gaattgcttt cggaatcgca cagtaaacca caaagtcttc
     7681 ctttttggac cacacacatt tttcgggcaa gccgccagaa cccaagttgg tgaccgcatg
     7741 atccttgatc gacaaaacct tgagaccgaa ttctttttcg gagggatttg catagagtac
     7801 cctcgaaagt gagttattta tgagtgccgt cagacccaaa ccatccataa ttttttcggt
     7861 cacttttgtc ttaaggttat gaaggtatag cgtacccctc actgttcccg agggttttgt
     7921 cgttaaggcg aggtgtgttg ccttaggcca ggcaatttgc cactccccca aatctgaatt
     7981 aaagacttca gttcgcttgg tttcgtcgtt tttggcagta taaccgactg accgctcccc
     8041 ttcagggata taaaaaatca aatcgtctga ctcgctcgga atcaaaactt ttaaattacg
     8101 aggtagaaac tttccctcaa ttttaaatgg cacgctgttt tctcctgtgt cctcgatggt
     8161 agaggtagct cgcggaataa gggtcgcaga atatgattgt attgtcttcc catcttggtc
     8221 gagatatcgc gctataaaac cggtaccgct cgcgagccac agtgcctctg tgattttagg
     8281 tatggtcgtg tttgaaatac gagagatgct tggatcttcg gtggagccgc gaaaaacgtg
     8341 tccggtcgct tggtccacga aaagtatttc tgtttcgttc tcgatgtcga tggctactgc
     8401 tccagcttgg ggaagtgaag aaatttttcg aagcgtagca agcggtgaac ttgagaggtt
     8461 atcattattc tgcccacctt gcggttcggg ctgtgctata ggtggtgtgc ctcttccacc
     8521 gaaatcaatc gttatatctt tttcctcggt agtaggaacg tcgactggtt tattctctcg
     8581 cattaacgtg aaaaagaata tgccaaggcc ggcgaagaca ataaggagaa tgaagattat
     8641 tagtaatgtt gcgtgttttc cgttcatccc gttagagacc cgcctgccat cgcctgacac
     8701 actgatggga ggacgtgtga ttaattggta atatcatctt cttgtactaa agctactgcg
     8761 tatttcgctc tggctgtggc gggcaggtag gaaatgctcc gcttatttgc ggaacattca
     8821 ctatattcaa ttaacaataa atttgatgca aaatttttta aaacggatag gcatttccgt
     8881 aacatatttt gataatgtga tgttgaacga ttgaatcgtg tagtcactag taagtaatta
     8941 tacataacta tgcaatttct aacgggattc attgattagt tttttatttt aatctttcga
     9001 gaaccaactt tttatcgaga gagtggattt acgagcccgg tagcggcact atgtcggtac
     9061 gcacttgcgg cttcgttagc tgtgtattga cgtccattat aaggaacttc tcggccgttt
     9121 accgtaatat tcacgggtac actgccgaat gttgtatatc taatgtcggc accggacggc
     9181 acggatatac cattttgaat tgcgagttcc ttcgcgcgtt ccctcgtgag cagggccgga
     9241 tcagtcgttg cactgtaacc ctgcctcatc atctcgtcat atttgtaaat caagtctcta
     9301 tcgggtacgg tgtttaccga aggaggcgga ttagctccgc ttgctccggg gagaggtaaa
     9361 actgggtaac ttattgggcc gtctgtttta ggatactcga gtggtttaaa ctgaagcaca
     9421 tccttgttaa ctgtgtagag aataagccaa gctgccatag ccagtataag gccacctatg
     9481 gccttactga ttatttcctt gccgtccttc ttgccttcga tagagtcgaa cgtcatataa
     9541 gtgaacccgc cccagacgat gcgaaatacc gcaagcactc ctgccgcggc aatggcaagt
     9601 ttaaaagcgc cgacaagaaa tggtaccagt tcataacttg tgaagaaacc tgtatccgtc
     9661 aagtcgacga gcggtgtata gtcggcttgc gcagcggttt tgtgagtaaa tgcgaaaaac
     9721 atagatagca ttccgataac aattttcaaa atagattttt ttatgggttc agttcgatcc
     9781 atagagaagt tttatgcgcg cacttgaaaa ctgcaaaaat ttactgctgt ttgaaaatat
     9841 aacgctaatt tgcgactcgc cgcgcgaatt ggcctctacc ccaaatgttc taaatggcgt
     9901 atcaccctcg agtaactctt cgttttgttt ccatttatac gcaaaaccgc ttgagtccgg
     9961 tgaactccaa tatggagcgg cgaaaaaccg tacctcgggt gcttttaaga ggaaacttcc
    10021 aatgagtgcc cgattttgct taatgccgag aatggggtca tcctcataga gtaaaagaag
    10081 cggctctgtc acgggtattg tgatgctgtc tcgaactcga agactgttat cggtagtgac
    10141 cacatcaagg tcgacaattc cctgaatgga ggtcgaagg
//
